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1 on (40-50%) in release of a secreted enzyme (invertase).
2 ters and a putative beta-fructofuranosidase (invertase).
3 lation of SbVIN1, a gene encoding a vacuolar invertase.
4 and the high mannose-containing glycoprotein invertase.
5 unoisolated clathrin-coated vesicles contain invertase.
6 r the endodermis and phloem for soluble acid invertase.
7 a suitable support for the immobilisation of invertase.
8 Pex15p was fused to the N-terminus of mature invertase.
9 lix that controls these steps by the Hin DNA invertase.
10 da repressor protein to the secreted protein invertase.
11 nts are shuffled by Rci, a site-specific DNA invertase.
12 reakdown through sucrose synthase instead of invertase.
13 ished their studies of sucrose hydrolysis by invertase.
14 fect on the retained activity of immobilized invertase.
15 aride must first be hydrolysed by the enzyme invertase.
16 d prokaryotic serine site-specific resolvase-invertases.
17 terminal domains compared with the resolvase/invertases.
18 rved for the differentially sugar-responsive invertases.
19 o each other and the FimE and HbiF bacterial invertases.
20 nthetic genes may have evolved from vacuolar invertases.
21 s encoding a SWEET transporter and cell wall invertases.
23 nthase activity and reduced Suc synthase and invertase activities, leading to increased Suc contents.
28 loem girdling on (1)(3)C and (1)(5)N import, invertase activity and polyphenol accumulation in juveni
30 enile tissues was positively correlated with invertase activity at the treatment site and enhanced by
32 carrot lines revealed very low acid-soluble invertase activity in rs/rs roots whereas neutral invert
37 orms a boundary, compartmentalizing apoplast invertase activity to allow different embryo and endospe
43 ucrose and starch hydrolysis (e.g. cell wall invertase, alpha-amylase, and starch phosphorylase) were
45 invertase) of the signal toward its analyte invertase and a negligible nonspecific interaction of th
49 identically to wild type and show delays of invertase and Gas1p ER-to-Golgi transport identical to t
50 e Y trafficking defect, but the secretion of invertase and gp400/hsp150 is not significantly affected
51 temperature; however, other proteins (i.e., invertase and HSP150) in these and other COPI mutants we
52 ulate an internal pool of fully glycosylated invertase and mature alpha-factor, while processing and
55 ptide followed by the coding region of yeast invertase and the transmembrane domain and cytoplasmic t
56 nd rse1-1 mutants accumulate the ER forms of invertase and the vacuolar protease CPY at restrictive t
57 ular C terminus can be processed to secreted invertase and this fraction is constrained to 2-3% by a
58 ncodes an integrase related to the resolvase/invertases and is evolutionarily and mechanistically dis
59 apid change in the regulation and balance of invertases and Suc synthases that could have an immediat
62 ndent fashion, the transcription of vacuolar invertase, and a wak2 mutant alters the normal pectin re
63 y owing to a jasmonic acid-induced cell wall invertase, and is limited by phloem sucrose availability
64 ferent sets of cargoes typified by Bgl2p and invertase are delivered to the plasma membrane for secre
72 invertase-repressor fusions, like wild-type invertase, behave as soluble proteins in the ER lumen.
73 inetics was based on the properties of yeast invertase (beta-fructo-furanosidase, EC 3.2.1.26), whose
75 l studied serine recombinases, the resolvase/invertases, bring two recombination sites together in a
76 stingly, CPY-invertase hybrid proteins, like invertase but unlike CPY, escaped the sec21 ts mutant ER
77 group, are similar in size to the resolvase/invertases but have the DNA binding domain N-terminal to
78 ing an Och1p-invertase fusion do not secrete invertase, but those expressing an Och1p-Kex2p site-inve
79 e at about a 2-fold molar excess inactivated invertase by modifying both of the enzyme's cysteine res
80 Rapid repression of the Ivr1 and Ivr2 maize invertases by low oxygen was evident in root tips within
84 ut activities of neutral invertase, vacuolar invertase, cell wall-bound invertase, fructose kinase, a
85 , released from wheat fructan and sucrose by invertase, compared to maltose is, however, not document
86 is based on target-dependent binding of cDNA-invertase conjugate with the analyte DNA, thereby transf
92 forming the resulting fusion library into an invertase-deficient yeast strain and plating the transfo
93 esults demonstrate that unique resolvase and invertase derivatives can be developed to site-specifica
96 low temperature, defects in glycosylation of invertase, dominant lethality, fluoride sensitivity, and
99 ic isotope fractionation associated with the invertase (EC 3.2.1.26) and glucose isomerase (EC 5.3.1.
103 Man10GlcNAc and Man11GlcNAc species from invertase expressed in Pichia pastoris showed three and
104 Finally, we note that glucose repression of invertase expression in wild-type cells produces a strat
106 ugh incomplete cell separation, 10 increased invertase expression, none imported sucrose, and 11 incr
109 catalytic domain derived from the resolvase/invertase family of serine recombinases and a custom-des
110 Hyperactivated variants of the resolvase/invertase family of serine recombinases function without
111 catalytic domains derived from the resolvase/invertase family of serine recombinases fused to Cys2-Hi
116 od is based on the target-induced release of invertase from a functional-DNA-invertase conjugate.
119 ion and characterization of an intracellular invertase from Trichoderma virens (TvInv) important for
120 es and involves at least three conserved DNA invertases from two evolutionarily distinct families.
121 vertase, vacuolar invertase, cell wall-bound invertase, fructose kinase, and hexokinase were unaffect
124 se, but those expressing an Och1p-Kex2p site-invertase fusion protein secrete high levels of invertas
125 ll surface protein can synthesize a secreted invertase fusion protein that can rescue the mutant, and
126 ansformed with the promoter of an apoplastic invertase gene (invGE) linked to a reporter gene also re
127 1, 2 and 3) showed that sequences for maize invertase gene and for events MON810 and TC1507 were eas
130 ic fruit expressing a constitutive antisense invertase gene had increased sucrose and decreased hexos
131 no effect on the expression of the cell wall invertase gene in fluorescent carrot cells containing ar
132 cDNA cloning vector which carries a modified invertase gene lacking its leader sequence is used in co
133 rate that silencing the potato vacuolar acid invertase gene VInv prevents reducing sugar accumulation
135 ealed asymmetric induction of one maize acid invertase gene, Ivr2, consistent with transcriptional re
137 m the phloem to the apoplast where cell wall invertases generate monosaccharides for uptake and utili
139 ed together with multiple copies of vacuolar invertase genes and a transposable element on two barley
141 rtase genes in potato, like most other plant invertase genes, include a very short second exon of 9 b
143 hich differs from previously described plant invertase genes; while intron locations are conserved be
145 mming of the DNA sequence specificity of the invertase Gin from bacteriophage Mu and Tn3 resolvase fr
146 ed activated mutant (M114V) of the G-segment invertase (Gin) in which one dimer half is rotated by 26
148 lta gal83Delta strain is unable to derepress invertase, grows poorly on alternative carbon sources an
157 Transgenic plants were generated with yeast invertase in the cell walls to prevent Suc loading by th
159 stolons revealed a marked decline in soluble invertase in the subapical region of swelling stolons, c
161 itation led to a down-regulation of vacuolar invertases in all plants, which resulted in an augmentat
163 ic analysis of the fructosyltransferases and invertases in the Poaceae showed that the fructan biosyn
164 belongs to a small gene family of apoplastic invertases in tomato (Lycopersicon esculentum), is a qua
166 which includes transposon resolvases and DNA invertases, in that they utilize two simple but differen
167 Miniature1 (Mn1) locus encodes the cell wall invertase INCW2, which is localized predominantly in the
168 on at the Mn1 locus that encodes a cell wall invertase (INCW2) that localizes exclusively to the basa
169 ed in a 3, 000-fold reduction in the kcat of invertase indicating that Glu-204 plays a major role in
171 nsported by dense exocytic vesicles, such as invertase, into light exocytic vesicles, whereas transpo
172 sucrose in cells in which the only source of invertase is a C-terminal fusion to a transmembrane prot
174 mutant cheater strain that does not produce invertase is able to take advantage of and invade a popu
176 ged fusion proteins was constructed in which invertase is appended to the Golgi-luminal carboxy termi
177 roteomic approach demonstrates that vacuolar invertase is glycosylated at all twelve potential N-glyc
180 Piv, a unique prokaryotic site-specific DNA invertase, is related to transposases of the insertion e
181 e in Saccharomyces cerevisiae, which encodes invertase, is repressed about 200-fold by high levels of
183 s not occur in the Rs wild-type acid-soluble invertase isozyme II allele, it does occur elsewhere in
188 44% of the sugars consumed were generated by invertase-mediated degradation of fructan, raffinose and
189 sess glucose flux differences between cells, invertase-mediated sucrose hydrolysis in vivo, delivery
191 TB2 reduced inclusion/splicing of the potato invertase mini-exon splicing reporter, indicating that t
193 a Kex2p cleavage site between the Och1p and invertase moieties to monitor transit to the Kex2p-conta
194 to distal compartments is not induced by the invertase moiety, since noninvertase fusion constructs e
200 ensor was only 6.1% (probed with an oxidized invertase) of the signal toward its analyte invertase an
201 uces the expression and activity of vacuolar invertase, often a key factor in turgor and expansion.
204 he major pilin subunits (tfpQ/I) and the DNA invertase (piv), which determines pilin type expression.
205 designed and highly convergent resolvase and invertase populations in the context of engineered zinc-
206 ne fetuin, bovine thyroglobulin, and several invertase preparations from wild-type and mutant yeast s
207 n be imported into the cell, serving to make invertase production and secretion a cooperative behavio
209 sed appropriately upstream of this defective invertase provide the necessary signals to restore secre
211 stream partially prevented the abortion, but invertase regulated the synthesis of ovary starch and pa
212 is adjacent to mpi, encoding the global DNA invertase regulating capsular polysaccharide biosynthesi
214 terest to a yeast (Saccharomyces cerevisiae) invertase reporter gene, transforming the resulting fusi
216 amily of site-specific recombinases--the DNA invertase/resolvase family--that catalyze inversion or d
217 cysteines with alanines revealed that C108A invertase retained full activity whereas C205A was reduc
218 ) released with peptide-N-glycosidase F from invertase secreted by Deltaalg9 yeast showed its structu
220 effects of ste24- and spf1-null mutations on invertase secretion are additive, cell generation time i
221 rictive temperature and exhibit a pattern of invertase secretion comparable with sec14(ts) strains.
226 xtensively glycosylated, indicating that the invertase segment inserted at these Akr1p sites is lumin
229 mportant physiological role of Saccharomyces invertase (SInv) and the historical relevance of this en
232 ere motivated by the potential for a reduced invertase/Suc synthase balance to alter the impact of re
234 tase activity in rs/rs roots whereas neutral invertase, sucrose synthase and sucrose phosphate syntha
235 here that invertase gene expression and the invertase-sucrose (Suc) synthase ratio decrease abruptly
236 we report that FimX, an ExPEC-associated DNA invertase that regulates the major virulence factor type
238 ructural groups represented by the resolvase/invertases, the large serine recombinases and relatives
240 where sucrose is hydrolysed by an apoplasmic invertase to produce a mixture of sucrose, glucose and f
241 own to rapidly encounter the TGN glycosylate invertase to the same extent as wild-type cells, indicat
242 ) plants expressing a constitutive antisense invertase transgene grew identically to wild-type plants
243 sis of a deletion mutant of one of these DNA invertases, tsr15 (aapI), which resulted in the promoter
244 ression is regulated at one level by the DNA invertase Tsr19, which is encoded by a gene immediately
246 in silenced fruit, but activities of neutral invertase, vacuolar invertase, cell wall-bound invertase
248 mal amplification method with a thermostable invertase, we can directly transduce Middle-East respira
250 gamma delta resolvases and the phage Mu Gin invertase, we used substrates that provided some but not
251 ranscripts for sucrose synthase and vacuolar invertase were both observed in the same cortical cells
253 he structure and the catalytic properties of invertase were preserved, while Km values were slightly
254 dified secretory pathway cargos, Hsp150p and invertase, whereas stt4(ts) cells exhibit no detectable
255 ypeptidase Y, as well as in the secretion of invertase, which accumulates as a core-glycosylated form
257 the association of transcripts for vacuolar invertase with polyribosomes did not change over this pe
258 orresponded to genes encoding a soluble acid invertase with potential vacuolar targeting, which we ge
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