ライフサイエンスコーパス: involucrin

1 cteristic of its final state (keratin 14 and involucrin).

2 atinocyte differentiation markers (loricrin, involucrin).

3 g later markers (profilaggrin, loricrin, and involucrin).

4 nnexin 43 (Cx43), cytokeratin K10 (K10), and involucrin.

5 lacement of K10 and increasing expression of involucrin.

6 ed keratins 1 and 6, filaggrin, loricrin and involucrin.

7 nd gain in a terminal differentiation marker involucrin.

8 entiation-associated target genes, including involucrin.

9 pression of late-stage markers, loricrin and involucrin.

10 nified envelope proteins, most abundantly to involucrin.

11 ast cells, or in the epidermal expression of involucrin.

12 d the expression of loricrin, filaggrin, and involucrin.

13 turation were assessed by immunostaining for involucrin.

14 activity of the differentiation marker gene, involucrin.

15 nd DNMT3B and negatively with p16(INK4A) and involucrin.

16 of the cornified envelope precursor protein involucrin.

17 shed CE precursor proteins SPR1A, SPR1B, and involucrin.

18 creased expression of the structural protein involucrin.

19 differentiation, as assayed by expression of involucrin.

20 ssion of the suprabasal markers loricrin and involucrin.

21 fferentiation markers such as keratin 10 and involucrin.

22 e SC and enhanced expression of loricrin and involucrin.

23 of differentiation-associated keratin 10 and involucrin.

24 elium and express the differentiation marker Involucrin.

25 the adult vagina, E(2) induced expression of involucrin, a CCAAT/enhancer-binding protein beta and cy

26 Involucrin, a cornified envelope precursor, and the cros

27 related with a decrease in the expression of involucrin, a differentiation marker.

28 es of keratin 5, a proliferative marker, and involucrin, a differentiative marker, respectively.

29 explore the impact of KLF4 on expression of involucrin, a gene that is specifically expressed in dif

30 Involucrin, a marker of epithelial differentiation, and

31 er activity and endogenous protein levels of involucrin, a marker of keratinocyte terminal differenti

32 ophoretic mobility supershift assay using an involucrin activator protein 1 (AP1) response element se

33 Involucrin also binds to SLV containing 12-18% phosphati

34 f differentiation and in contrast to that of involucrin, an early marker of terminal differentiation,

35 ncestral glutamine-glutamate-rich regions of involucrin, an important CE structural protein.

36 arize the literature regarding regulation of involucrin, an important marker gene that serves as a mo

37 etween specific glutaminyl residues of human involucrin and a synthetic analog of epidermal specific

38 Using involucrin and an epidermal omega-hydroxyceramide analog

39 y of the VDRE promoter and the expression of involucrin and CYP24 mRNA.

40 osition of membranes, transglutaminases, and involucrin and envoplakin in the initiation of CE assemb

41 containing lipid Z, lipid Z was attached to involucrin and formed saponifiable protein-lipid adducts

42 ydrocortisone in vitro induced expression of involucrin and high-molecular-mass CKs that are characte

43 his was featured by decreased E-cadherin and involucrin and increased vimentin and integrin beta(1).

44 20(OH)D3 stimulated involucrin and inhibited cytokeratin 14 expression.

45 ed expression of the differentiation markers involucrin and keratin 10 compared to cells with no cell

46 ssion pattern of the differentiation markers involucrin and keratinocyte transglutaminase.

47 lls at the apical surface; the expression of involucrin and keratins 6, 13, 14, and 19; and the absen

48 melatonin and AFMK-stimulated expression of involucrin and keratins-10 and keratins-14 in the epider

49 rneum junction and a modest decrease in both involucrin and loricrin protein expression, markers of k

50 ged, whereas the cornified envelope proteins involucrin and loricrin were increased in ft/ft epidermi

51 ecreased expression of K1 and K10 but not of involucrin and loricrin.

52 affected in nkt skin, with overexpression of involucrin and profilaggrin/filaggrin along with focal a

53 cornified envelope (CE) precursor proteins (involucrin and small proline-rich [Sprr] -1a, -1b, -2a,

54 quamation of and cornified envelope protein (involucrin and small proline-rich protein [SPRR]-2) expr

55 es for lipid synthesis and the expression of involucrin and small proline-rich proteins, which covale

56 , improved corneal smoothness, and decreased involucrin and SPRR-2 immunoreactivity compared with EDE

57 stress significantly increased expression of involucrin and SPRR-2 in the corneal epithelia.

58 We propose a model in which involucrin and TGase 1 bind to membranes shortly after e

59 However, on SLV carrying both involucrin and TGase 1, only five glutamines serve as do

60 glitazone, a PPAR-gamma activator, increases involucrin and transglutaminase 1 mRNA levels.

61 mRNA and protein levels of involucrin and transglutaminase 1, markers of differenti

62 rotein levels of the differentiation markers involucrin and transglutaminase following administration

63 Levels of involucrin and transglutaminase mRNA and protein were in

64 ation of the calcium-stimulated increases in involucrin and transglutaminase mRNA levels.

65 substantial reduction in calcium-stimulated involucrin and transglutaminase promoter activities.

66 The results showed that involucrin and transglutaminase protein and mRNA levels

67 e also activators of PPARalpha, also induced involucrin and transglutaminase protein and mRNA.

68 of the keratinocyte differentiation markers involucrin and transglutaminase to 1,25-dihydroxyvitamin

69 While induction of the spinous layer markers involucrin and transglutaminase was compatible with late

70 ed expression of the differentiation markers involucrin and transglutaminase were also blocked by the

71 , as demonstrated by increased expression of involucrin and transglutaminase, and inhibited prolifera

72 tion of keratinocyte differentiation markers involucrin and transglutaminase.

73 nly one lysine and two glutamine residues of involucrin and two glutamines of envoplakin were used in

74 inocyte differentiation (cytokeratin K10 and involucrin) and markers of apoptosis (TUNEL and anticasp

75 n epithelial structural integrity (e.g. Ivl (involucrin) and Sbsn (suprabasin)).

76 f epithelial differentiation marker protein (involucrin), and change of cell cycle position.

77 inocyte differentiation (cytokeratin K10 and involucrin), and markers of apoptosis (TdT-mediated dUTP

78 f the squamous differentiation markers Spr1, involucrin, and cytokeratin 1.

79 litazone or troglitazone increases loricrin, involucrin, and filaggrin expression without altering ep

80 rabasal keratin 10, transglutaminase type I, involucrin, and filaggrin.

81 XA7 expression activated transglutaminase 1, involucrin, and keratin 10 message and protein levels, d

82 differentiation markers, such as filaggrin, involucrin, and loricrin, was slightly increased in PPAR

83 ssion of one of these cross-linked proteins, involucrin, and that this effect can be abolished by mut

84 elium is stratified and is positive for K14, involucrin, and TRP63, but negative for keratin 10.

85 Ca2+-sensitive involucrin AP-1 promotor activity was increased, both in

86 Filaggrin (FLG), loricrin (LOR), and involucrin are important epidermal barrier proteins.

87 ed that key differentiation genes, including involucrin, are bound to heavy polysomes during differen

88 entiation proteins, filaggrin, loricrin, and involucrin, became abnormal.

89 Preferential involucrin biotinylation and the increased cornified cel

90 It resembles the involucrin coding region of other non-anthropoid mammals

91 rentiating agent on the promoter activity of involucrin, consistent with promotion of early different

92 fundibula expressed filaggrin, profilaggrin, involucrin, cornifin alpha, and loricrin.

93 GF-2, IFNalpha2, IL-1RA, HSA, keratin-6, and involucrin; cortisol was significantly higher (p < 0.05)

94 wever, to date, direct isolation from CEs of involucrin cross-linked by way of the transglutaminase-i

95 sequencing revealed many peptides involving involucrin cross-linked either to itself or to a variety

96 08 and glutamines 465 and 489 for interchain involucrin cross-links.

97 cies represents an early intermediate in the involucrin crosslinking process.

98 ntiation marker keratin K1 and inhibition of involucrin crosslinking.

99 neage-tracing studies in combination with an involucrin-driven Cre/lox reporter system confirmed that

100 Involucrin, envoplakin, and periplakin form the protein

101 In CEs of 3-d cultured cells, involucrin, envoplakin, and small proline-rich proteins

102 Together, these data indicate that involucrin, envoplakin, periplakin, and possibly other s

103 barrier proteins-envoplakin, periplakin, and involucrin (EPI-/- mice)-have a defective cornified laye

104 barrier proteins-envoplakin, periplakin and involucrin-(EPI-/- mice) have a defective cornified laye

105 on microscopy, this exposed large amounts of involucrin epitopes as well as of desmoplakin, a desmoso

106 ts keratinocyte differentiation, we assessed involucrin expression in HHD keratinocytes.

107 Involucrin expression is tightly linked to the onset of

108 orbol-13-acetate-dependent increase in human involucrin expression, and PRMT5 dimethylates proteins i

109 ed for appropriate differentiation-dependent involucrin expression, and that the mechanism of regulat

110 ng the action of 1,25-dihydroxyvitamin D3 on involucrin expression, but the vitamin D response elemen

111 alcium or vitamin D-induced up-regulation of involucrin expression, suggesting that the enzymatic act

112 to the timing and requirements for Aire and involucrin expression, the latter a marker of terminally

113 augments the PKCdelta-dependent increase in involucrin expression, whereas KLF4 knockdown attenuates

114 tosidase staining follows that of endogenous involucrin expression.

115 region of the promoter required for in vivo involucrin expression.

116 scriptionally inactive forms do not increase involucrin expression.

117 y inhibition of calcium or vitamin D-induced involucrin expression.

118 tern-regulated cell-cell contact to modulate involucrin expression.

119 by pattern size, did not alter keratinocyte involucrin expression.

120 including decreased keratin-14 and increased involucrin expression.

121 anscription factors known to be required for involucrin expression.

122 ent of the epidermis revealed a reduction in involucrin, filaggrin, and loricrin-markers of different

123 P63, C/EBPdelta, CK10 and involucrin fluorescence combined with morphology observa

124 The vitamin D response element from the involucrin gene bound the vitamin D receptor and the ret

125 Regulation of human involucrin gene expression and promoter activity was ass

126 factors and signaling cascades in regulating involucrin gene expression are presented.

127 y region AP1-5 element, in the regulation of involucrin gene expression during corneal epithelial cel

128 ut is absolutely necessary for activation of involucrin gene expression in the differentiating cornea

129 Involucrin gene expression is initiated early in the dif

130 In conclusion, calcium-regulated involucrin gene expression is mediated at least in part

131 urcumin and EGCG produce opposing effects on involucrin gene expression via regulation of C/EBP facto

132 Regulation of involucrin gene expression was monitored in cultures of

133 n promoter or prevent calcium stimulation of involucrin gene expression, but blocked 1,25-dihydroxyvi

134 the impact of specific promoter mutations on involucrin gene expression.

135 and proximal-regulatory regions, to regulate involucrin gene expression.

136 regulation of human corneal epithelial cell involucrin gene expression.

137 omplete differentiation-dependent program of involucrin gene expression.

138 ducer of keratinocyte differentiation and of involucrin gene expression.

139 The coding region of the involucrin gene of Tupaia glis has been cloned and seque

140 Previous studies show that the human involucrin gene promoter has two distinct regulatory reg

141 ining HSEs and STREs that are present in the involucrin gene promoter.

142 In the present study, the involucrin gene was used as a model to study this regula

143 In the present study, the involucrin gene was used as a model to study this regula

144 within the distal 5'-flanking region of the involucrin gene which contributes to differentiation-dep

145 ncreased AP-1-dependent transcription of the involucrin gene, an effect that may be mediated by liver

146 al promoter activity and TPA response of the involucrin gene.

147 ssion, and transcriptional regulation of the involucrin gene.

148 of the consensus sequence of four mammalian involucrin genes comprises four pairs of complementary o

149 popeptides, the ester linkage formation used involucrin glutamine residues 107, 118, 122, 133, and 49

150 This cascade activates transcription of involucrin (hINV) and other genes associated with differ

151 delta (PKCdelta) is a key regulator of human involucrin (hINV) gene expression and is regulated by ty

152 ) isoforms are important regulators of human involucrin (hINV) gene expression during keratinocyte di

153 ein kinase C (PKC), Ras, and MEKK1 regulates involucrin (hINV) gene expression in epidermal keratinoc

154 ggest that a PKC/Ras/MEKK1 cascade regulates involucrin (hINV) gene expression in human epidermal ker

155 te differentiation as measured by effects on involucrin (hINV) gene expression.

156 In the present study, the human involucrin (hINV) gene was used as a model to study gene

157 expression of the AP1 factor-regulated human involucrin (hINV) gene.

158 Human involucrin (hINV) is a keratinocyte protein that is expr

159 Involucrin (hINV) is a marker of keratinocyte differenti

160 Human involucrin (hINV) is a precursor of the keratinocyte cor

161 Human involucrin (hINV) is a structural protein that is select

162 Human involucrin (hINV) is a structural protein that is select

163 Involucrin (hINV) is an important structural component o

164 Human involucrin (hINV) mRNA level and promoter activity incre

165 The KLF4 induction of human involucrin (hINV) promoter activity is mediated via KLF4

166 s, increased binding of these factors to the involucrin (hINV) promoter, and increased expression.

167 factors suppress transcription of the human involucrin (hINV) promoter.

168 Expression of involucrin (hINV), a marker of keratinocyte differentiat

169 Human involucrin (hINV), first appears in the cytosol of kerat

170 of the keratinocyte differentiation marker, involucrin (hINV), via a Ras, MEKK1, MEK3, p38delta sign

171 skin keratinocytes releases several discrete involucrin-immunoreactive peptides.

172 Ca2+(o)-induced expression of keratin 1 and involucrin in HEKs.

173 Ca2+(o)-induced expression of keratin 1 and involucrin in HEKs.

174 ed decreased expression of Krt86, Krt6b, and involucrin in the epidermal portion of the claw field in

175 mRNA levels for loricrin, profilaggrin, and involucrin in the outer epidermis, but protein levels di

176 ne expression driven by a cellular promoter (involucrin) inserted in an internal position in the retr

177 s required for cornified envelope formation, involucrin (INV) and transglutaminase, increased 2- to 3

178 otein levels of transglutaminase (TGase) and involucrin (INV) over time in culture.

179 pression of Cx26 from the epidermis-specific involucrin (INV) promoter (INV-Cx26) demonstrated that d

180 y to basal, proliferating keratinocytes; the involucrin (Inv) promoter targeted the receptor to supra

181 Involucrin is a major protein of the cornified envelope

182 Involucrin is a major protein of the cornified envelope

183 Involucrin is a marker of human keratinocyte differentia

184 Involucrin is a marker of keratinocyte terminal differen

185 Involucrin is a protein that makes up the cornified enve

186 ucrin provide experimental confirmation that involucrin is an important early scaffold protein in the

187 Involucrin is an integral component of the cornified env

188 Involucrin is expressed in the differentiated suprabasal

189 Although the function and evolution of involucrin is known, the regulation of its gene expressi

190 ggest that when the central segment of human involucrin is predominantly alpha-helical, accompanied b

191 S5 and early differentiation markers such as involucrin (IVL) and cytokeratin CK13 in a CSL-dependent

192 This included genes such as involucrin (IVL), keratinocyte differentiation-associate

193 Thus, involucrin joins the ranks of a small set of genes that

194 ile of the epidermal differentiation markers involucrin, keratin 10, and filaggrin during tissue reco

195 Both SPRR2 and involucrin levels accumulated in the presence of MDC.

196 were detected in the cytosolic fraction, and involucrin levels increased after UVB.

197 The expressions of involucrin, loricrin, and cathepsin L is initially incre

198 necessary for cornified envelope formation, involucrin, loricrin, and filaggrin, and the activity of

199 pression of terminal differentiation markers involucrin, loricrin, and filaggrin.

200 oxysterol treatment increased the levels of involucrin, loricrin, and profilaggrin protein and mRNA

201 erexpression and knockdown studies show that involucrin mRNA and protein level correlates directly wi

202 A, in turn, was caused by increased rates of involucrin mRNA degradation.

203 f cornified envelope formation, reduction of involucrin mRNA expression, and transcriptional regulati

204 Our previous studies show that involucrin mRNA levels are increased by the keratinocyte

205 volucrin protein levels were caused by lower involucrin mRNA levels in HHD keratinocytes.

206 t PPARalpha activators induce an increase in involucrin mRNA levels.

207 n of Kdap mRNA expression similar to that of involucrin mRNA, but with differing kinetics.

208 Decreased involucrin mRNA, in turn, was caused by increased rates

209 ntrolled--demonstrates that the emergence of involucrin(+) mTECs critically depends upon the presence

210 Here we demonstrate that both involucrin-negative and involucrin-positive cells are ab

211 6 microm per h) of the mean rate achieved by involucrin-negative cells (46.5 microm per h).

212 in wounds, it is likely that both the basal, involucrin-negative cells and the involucrin-positive su

213 e specific AP-1 proteins, thereby activating involucrin, one of the genes required for epidermal diff

214 holipase C-gamma1 construct showed decreased involucrin or transglutaminase promoter activity in resp

215 Cotransfection of keratinocytes with the involucrin or transglutaminase promoter construct and th

216 erase reporter vectors containing either the involucrin or transglutaminase promoter, the antisense C

217 with a luciferase reporter vector containing involucrin or transglutaminase promoters led to a substa

218 2 had no effect on protein or mRNA levels of involucrin or transglutaminase.

219 Involucrin plays an important role in the lipid and prot

220 Despite their decreased migration rates, involucrin-positive cells appear to possess an intact me

221 emonstrate that both involucrin-negative and involucrin-positive cells are able to respond to a direc

222 The new observation that involucrin-positive cells can indeed migrate suggests th

223 The involucrin-positive cells, however, display mean migrati

224 ltures increased the number of MUC5AC(+) and involucrin-positive cells, which were blocked with the D

225 hannel that was found only in differentiated involucrin-positive cells.

226 the basal, involucrin-negative cells and the involucrin-positive suprabasilar cells respond to this c

227 the basal layer, or the more differentiated, involucrin-positive suprabasilar cells.

228 Epidermal differentiation markers, including involucrin, profilaggrin, and loricrin, detected by immu

229 epidermis there was decreased expression of involucrin, profilaggrin-filaggrin, and loricrin as assa

230 red with the epidermis of PPARalpha+/+ mice, involucrin, profilaggrin-filaggrin, and loricrin express

231 oteins of the upper spinous/granular layers (involucrin, profilaggrin-filaggrin, loricrin) increased

232 ion and distribution of cytokeratin K1, K14, involucrin, proliferating cell nuclear antigen, and p21c

233 knockdown of PKC-eta inhibits TPA-dependent involucrin promoter activation.

234 cholesterol increased transglutaminase 1 and involucrin promoter activity 2- to 3-fold.

235 tentiated the calcium-stimulated increase in involucrin promoter activity unlike NPS S-467 or vehicle

236 PKC-delta knockdown reduces TPA-activated involucrin promoter activity, nuclear activator protein-

237 cribed AP1 sites mediated Whn suppression of involucrin promoter activity.

238 We have sequenced the upstream region of the involucrin promoter and localized a calcium response ele

239 scription factor-binding site present in the involucrin promoter distal regulatory region is required

240 ad epidermis by showing that the full-length involucrin promoter drives differentiation-appropriate e

241 Within the distal regulatory region of the involucrin promoter lies an AP-1 site and an element hom

242 ement did not reduce basal expression of the involucrin promoter or prevent calcium stimulation of in

243 te and the vitamin D response element in the involucrin promoter play important roles in mediating th

244 scription factor DNA binding to two discrete involucrin promoter regions, the distal- and proximal-re

245 e regions in vivo, we have constructed human involucrin promoter transgenic mice and monitored the im

246 The effect of calcium on the involucrin promoter was enhanced synergistically by phor

247 epeat (LTR), the keratin 14 promoter, or the involucrin promoter was not altered, nor was expression

248 on of the -2452 bp to -1880 bp region of the involucrin promoter, or mutation of the AP-1 site within

249 ctivity and block calcium stimulation of the involucrin promoter, whereas the vitamin D response elem

250 The transgenic animals express whn from the involucrin promoter, which is active in keratinocytes un

251 ransfected with either transglutaminase 1 or involucrin promoter-luciferase constructs.

252 , MycERTAM, is targeted to epidermis via the involucrin promoter.

253 vator protein (AP)-1 response element in the involucrin promoter.

254 al keratinocytes of murine epidermis via the involucrin promoter.

255 ne transactivator), expressed from the human involucrin promoter.

256 1,25-dihydroxyvitamin D3 stimulation of the involucrin promoter.

257 1,25-dihydroxyvitamin D3 stimulation of the involucrin promoter.

258 e with desmoglein 3 under the control of the involucrin promoter.

259 press human factor VIII under control of the involucrin promoter.

260 igand-binding domain (ODCER) is driven by an involucrin promoter.

261 cornea tissue, SPRR1, SPRR2, filaggrin, and involucrin protein expression were detected in the centr

262 in mRNA levels, and filaggrin, loricrin, and involucrin protein levels all increased with air exposur

263 +, we found that these cells expressed lower involucrin protein levels at both low and high extracell

264 Decreased involucrin protein levels were caused by lower involucri

265 SPRR2 and involucrin protein levels were studied by immunofluoresc

266 In HCECs, SPRR2 and involucrin proteins were detected in the cytosolic fract

267 The multiple cross-linked partners of involucrin provide experimental confirmation that involu

268 Moreover, cotransfection of the human involucrin reporter plasmid with C/EBPalpha increases pr

269 mmunostaining for keratins 10 and 16 and for involucrin revealed an initial pattern of epithelial imm

270 ing of tryptic peptides from TGase 1-reacted involucrin showed a large increase in deamidation of sub

271 l known or novel barrier proteins, including involucrin, small proline-rich proteins, repetin, and ep

272 Nine genes including involucrin, SPRR (types 1A, 1B, 2A, 2B, and 3), late env

273 Levels of involucrin; Sprr-1a, -1b, -2a, -2b, -2f, and -2g; and Tg

274 signaling, continued mTEC development to the involucrin(+) stage maps to activation of the LTalpha-LT

275 g, including keratin 1, loricrin, filaggrin, involucrin, TGK, and SPR-1.

276 ad-to-tail and head-to-head cross-linking of involucrin to itself and to envoplakin and perhaps perip

277 teraction of TGase 1 with substrates such as involucrin to permit specific cross-linking for initiati

278 These results suggest that activation of involucrin transcription involves a pathway that include

279 the promoter for the differentiation marker involucrin, transgenic mice that ectopically express whn

280 le in hair shaft formation (trichohyalin and involucrin, ultra-high sulfur matrix proteins, and trans

281 cytokeratin K1 transglutaminase type I, and involucrin was increased in the absence of exogenous ret

282 The physiological transglutaminase substrate involucrin was preferentially biotinylated in situ, dete

283 d, expression of the EDC genes filaggrin and involucrin was strongly decreased directly by IL-13.

284 line-rich proteins (SPRRs) and filaggrin and involucrin was studied in human cornea sections by immun

285 Involucrin was the first protein to be identified as a l

286 tic digestion after saponification, of which involucrin was the most abundant.

287 n of the differentiation markers K1, K6, and involucrin were abnormal.

288 eratinization-related proteins filaggrin and involucrin were not expressed in normal conjunctival epi

289 When recombinant human TGase 1 and involucrin were reacted on the surface of synthetic lipi

290 By this time >15 residues of involucrin were used for cross-linking.

291 Specific glutamines or lysines of involucrin were used to cross-link the different protein

292 specific cytokeratins (CKs), filaggrin, and involucrin were used to define distinct stages of TE cel

293 EGQLEH, found in the central region of human involucrin, were studied by circular dichroism spectrosc

294 In reactions of involucrin with TGase 1 enzyme in solution, 80 of its 15