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1 ts: MTSET+, MTSES-) and an alkylating agent (iodoacetamide).
2 ree thiol molecules have been alkylated with iodoacetamide.
3 lenesulfonic acid and tetramethylrhodamine-5-iodoacetamide.
4 the photocross-linking reagent, benzophenone-iodoacetamide.
5 oxylic acid, N-ethylmaleimide, spermine, and iodoacetamide.
6 modification of cysteine residues with [14C]iodoacetamide.
7 Cys-961, Cys-995) were readily accessible to iodoacetamide.
8 in sequencing after modification with [1-14C]iodoacetamide.
9 ha-p-tosyl-L-lysine chloromethyl ketone) and iodoacetamide.
10 detected after treatment of the enzyme with iodoacetamide.
11 O(-), nitrated complex II was alkylated with iodoacetamide.
12 ble and were eliminated by pretreatment with iodoacetamide.
13 of actin labeled at Cys374 with erythrosine iodoacetamide.
14 s more reactive toward free cysteine than is iodoacetamide.
15 ing ERK2 proteins subjected to alkylation by iodoacetamide.
16 rential alkylation with N-ethylmaleimide and iodoacetamide.
17 e, 1-Cl-2,4-dinitrobenzene, and biotinylated iodoacetamide.
18 yme when thiolated by GSSG or alkylated with iodoacetamide.
19 tol followed by alkylation with radiolabeled iodoacetamide.
20 nd alkylated the free sulfhydryl groups with iodoacetamide.
21 eta-UP was very sensitive to inactivation by iodoacetamide.
22 d increased resistance against alkylation by iodoacetamide.
23 cleophosmin was inhibited in the presence of iodoacetamide (10 mM), free (+)-avrainvillamide (100 mic
26 We labeled alphaA-crystallin with stilbene iodoacetamide (4-acetamido-4'-((iodoacetyl)amino)stilben
27 with different cysteine alkylating reagents: iodoacetamide, 4-vinylpyridine, and (3-acrylamidopropyl)
28 ly alkylated with acrylamide (+71 Da) versus iodoacetamide (+57 Da) have substantial chromatographic
29 wo heterobifunctional photoaffinity reagents iodoacetamide 9 and dansyl derivative 10 were prepared.
30 in the apoenzyme and that the binding of the iodoacetamide (a truncated analogue of the citrulline pr
33 ilar results were obtained on the photolyzed iodoacetamide-alkylated yeast GR, which was evaluated by
34 of the catalytic cysteine (SufS(C361A)) and iodoacetamide alkylation of SufU support the occurrence
35 ver, the measurement of the pH dependence of iodoacetamide alkylation revealed a pK value of 6.6 for
37 nzyme of a thiol-reactive FRET probe, pyrene iodoacetamide, allowed us to monitor the binding by FRET
39 emically modified Cys and His residues using iodoacetamide and diethyl pyrocarbonate, respectively.
40 and Ba(OH)(2), blocking cysteine residues by iodoacetamide and enzymatic O-deglycosylation prior to D
41 ively, were each labeled with benzophenone 4-iodoacetamide and exchanged into myosin in their phospho
42 1 and Grx2 were sensitive to inactivation by iodoacetamide and H(2)O(2) and exhibited similar pH depe
43 n are not accessible to all electrophiles as iodoacetamide and internally applied 2-(trimethylammoniu
46 he implementation of an alkylating step with iodoacetamide and optimization of the incubation tempera
48 ysteine-34 of HSA was permanently blocked by iodoacetamide and the primary amines were temporarily ma
50 The cleavage of DSEB/RF-C140 is prevented by iodoacetamide and the specific caspase-3 inhibitor, tetr
52 sfer was inhibited by treatment of IscU with iodoacetamide, and (35)S was released by reducing reagen
53 induced modification of fibrillarin to 2-ME, iodoacetamide, and hydrogen peroxide suggested interacti
54 nzyme was inactivated when preincubated with iodoacetamide, and protected from inactivation when preQ
55 leimide but not to the thioreactive compound iodoacetamide; and importantly, low concentrations of Tr
56 copy of TnC((E59D,D75Y)) labeled with 2-[(4'-iodoacetamide)-aniline]naphthalene-6-sulfonic acid showe
57 Measurement of the enzymatic inhibition by iodoacetamide as a function of pH showed maximum inhibit
59 ysteine probes from chemical modification by iodoacetamide as a means to obtain a surface map of TIP4
60 e compounds, p-chloromercuribenzoic acid and iodoacetamide, as well as the structure of a cysteine to
62 d FRET in a series of mutants bearing pyrene iodoacetamide at different positions, and we measured th
64 rescence from the triplet probe erythrosin-5-iodoacetamide attached to sulfhydryls in rabbit skeletal
66 etry following chemical modification with an iodoacetamide-based reagent over a range of pH buffers.
67 he facts that H(2)O(2) and biotin-conjugated iodoacetamide (BIAM) selectively and competitively react
70 aglandin J(2), 2,4-dinitrochlorobenzene, and iodoacetamide] correlates with the selective modificatio
71 anisotropy of the triplet probe erythrosin-5-iodoacetamide covalently attached to cysteine 374 of act
72 ropy (rFA) of the triplet probe erythrosin-5-iodoacetamide covalently bound to Cys-374 of skeletal mu
75 mutants are hexa-alkylated upon addition of iodoacetamide, demonstrating accessibility and full reac
78 abeled at Cys-84 with tetramethylrhodamine-5-iodoacetamide dihydroiodide was passively exchanged for
79 30 minutes, the inhibition of CK activity by iodoacetamide does not limit left ventricular chamber co
80 ersible, time-dependent decrease in stilbene iodoacetamide emission intensity and a concomitant incre
84 8C, and S59C) were prepared, and fluorescein iodoacetamide (FI) was selectively conjugated to the sid
86 photoactivatable cross-linker benzophenone-4-iodoacetamide, followed by reconstitution and UV irradia
88 ure or metabolic inhibitors, sodium azide or iodoacetamide, have little influence on the translocatio
89 teine moiety is blocked by pretreatment with iodoacetamide, hemin binding is not affected, whereas dr
90 unrelated cysteine-modifying agents such as iodoacetamide (IA) and (2-aminoethyl)methanethiosulphona
91 Ten-day-old male rats received 0.2 mL 0.1% iodoacetamide (IA) in 2% sucrose daily by oral gavages f
92 ations by mPEG(5000)-maleimide (PEG-mal) and iodoacetamide (IA) under denatured condition, using extr
94 eta93Cys is modified by sulfhydryl reagents, iodoacetamide (IAA) and N-ethylmaleimide (NEM), respecti
95 ethyl]1-sulfo-5-naphthylamine (IAEDANS), and iodoacetamide (IAA) were used to selectively modify the
97 insensitive to iodoacetic acid (IAAcid) and iodoacetamide (IAAmide), though IAAmide-treated receptor
100 the effectiveness of N-ethylmaleimide (NEM), iodoacetamide (IAM), and iodoacetic acid (IAA) in alkyla
101 first block reduced thiols in extracts with iodoacetamide (IAM), and then we sequentially reduce and
102 on, the sulfhydryls are usually reacted with iodoacetamide (IAM), iodoacetic acid (IAA), or another e
103 comparison of three derivatization reagents: iodoacetamide (IAM), N,N-dimethyl-2-chloro-ethylamine (D
106 reagents such as N-ethylmaleimide (NEM) and iodoacetamide in a dose-dependent and time-dependent man
108 ammonium acetate (AmAc) in rat chow or 0.1% iodoacetamide in drinking water for 2-3 weeks underwent
109 and E2 cysteinyl residues were labeled with iodoacetamide in the native virus particle and analyzed
110 mutant enzymes, as shown by pH profiles for iodoacetamide inactivation of the respective enzymes, cl
112 Sp1 can be inhibited by N-ethylmaleimide and iodoacetamide, indicating that a cysteine protease media
113 ve, the pH-dependent incorporation of [(14)C]iodoacetamide into this mutant exhibits a pK(a) = 7.62.
115 cted agents, N-chlorosuccinimide, elastinal, iodoacetamide, iodoacetic acid, and phenylglyoxal gave s
116 , the pH-inactivation profile of 3CLpro with iodoacetamide is indicative of an ion-pair mechanism.
118 e monoethylene ketal in five simple steps to iodoacetamide ketone 10, which was cyclized in good yiel
119 icrosecond rotational dynamics of erythrosin-iodoacetamide-labeled actin strongly bound to single-hea
120 icrosecond rotational dynamics of erythrosin-iodoacetamide-labeled actin with strongly bound myosin V
122 lation caused a 48% decrease in biotinylated iodoacetamide-labeled Ras that was reversed by dithiothr
123 ications reflected by decreased biotinylated iodoacetamide labeling and evidence of specific irrevers
125 itrobenzoic acid) (Nbs2), and lucifer yellow iodoacetamide (LYI), suggests that sulfhydryl groups mig
126 on ionization time-of-flight spectroscopy of iodoacetamide-modified DmGCLM as well as examination of
128 plete mapping of total tryptic digest of the iodoacetamide-modified oat phytochrome A (phyA), the mol
129 cysteines cannot be distinguished with [14C]iodoacetamide, N-[(14)C]ethylmaleimide, or IAEDANS ([5-(
130 ity of the active-site Cys161 residue toward iodoacetamide nor the rate of unidirectional transfer of
131 D1s with TCEP increased the accessibility to iodoacetamide of cysteine residues that normally partici
132 in V-117 mutant was labeled with fluorescein iodoacetamide on its single N-terminal cysteine residue;
133 er induction of mild diffuse gastritis using iodoacetamide or creating gastric ulcers by injecting 60
134 al buffers, sulfinic acids do not react with iodoacetamide or disulfides, enabling selective alkylati
135 The demonstration that either treatment with iodoacetamide or replacement of the motif cysteine with
137 on of hydroxylamine-treated ASGP-R with [14C]iodoacetamide resulted in the specific incorporation of
138 t, the pH dependence of SrtA inactivation by iodoacetamide revealed a single ionization for inactivat
139 ed proteins treated with N-ethylmaleimide or iodoacetamide revealed that all cysteines form covalent
140 Derivatization of gammaS-crystallin with iodoacetamide showed reaction at only six of the seven c
141 luoroalkyl tag, N-[(3-perfluorooctyl)propyl] iodoacetamide, significantly increased their hydrophobic
144 group-specific protease inhibitors, such as iodoacetamide, suggest an active-site cysteine or histid
145 ed and solubilized in the presence of 100 mM iodoacetamide, suggesting that it is not unique to rat l
146 presence of the thiolate scavenging reagent, iodoacetamide, suggesting that the role of O2 is in oxid
148 of apoptosis, is blocked by the addition of iodoacetamide to cells prior to the onset of apoptosis,
149 67C was then treated with bromoethylamine or iodoacetamide to insert aminoethylcysteine (AEC) or carb
150 eta tubulin were alkylated with an excess of iodoacetamide to prevent artifactual generation of disul
151 This was achieved by applying a low dose of iodoacetamide to selectively inhibit the creatine kinase
153 be readily alkylated in the native SPL by an iodoacetamide treatment, suggesting that it is accessibl
154 d by reduction with NaBH(4), alkylation with iodoacetamide, trypsin digestion, and HPLC separation of
156 The fluorescent probe tetramethylrhodamine iodoacetamide was attached to cysteine residues substitu
158 te synthetase (FPGS) that were reactive with iodoacetamide were located in peptides that were highly
159 enzyme, we labeled SERCA with erythrosin 5'-iodoacetamide, which binds to the phosphorylation domain
161 cence lifetime of the actin-bound erythrosin iodoacetamide: while muscle S1 increases the lifetime, s
162 t not the oxidized enzyme, is inactivated by iodoacetamide with alkylation of 2.7 cysteine residues/s
163 ne serum albumin (BSA) was modified by (14)C-iodoacetamide, with and without glutathione present, pro
164 ce all 22 half-cystines can be modified with iodoacetamide without reduction of the chromoprotein, it
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