ライフサイエンスコーパス: isoalloxazine

1 s a unique interaction between Lys53 and the isoalloxazine.

2 are adjacent to the quininoidal edge of the isoalloxazine.

3 alysis via a number of different mechanisms, isoalloxazine analogues are valuable for mechanistic stu

4 configuration of the FAD cofactor, where the isoalloxazine and adenine rings are nearly in vdW contac

5 that the unique relative orientation of the isoalloxazine and adenine rings may have resulted from t

6 nalogue binds against the si face of the FAD isoalloxazine and is protected from bulk solvent by heli

7 s stacked parallel to the si face of the FAD isoalloxazine and positions the sulfenyl oxygen atom onl

8 P)H binds with the nicotinamide near the FAD isoalloxazine and the ADP moiety extending toward the mo

9 interactions between coplanar nicotinamide, isoalloxazine, and Phe309 rings; (ii) rearrangement of m

10 in substrate binding is the movement of the isoalloxazine between an "in" conformation and a more ex

11 rs ordering of the peptide loops so only one isoalloxazine can fit.

12 , between coenzyme analog, NAD(P)H4, and the isoalloxazine center in the model flavoenzymes morphinon

13 in the pyrazine and pyrimidine rings of the isoalloxazine core of the cofactor from readily availabl

14 ncluding 20 degrees butterfly bending of the isoalloxazine, crankshaft rotation of the ribityl, shift

15 e pathway for molecular oxygen to access the isoalloxazine group for the oxidative half reaction.

16 dds a fourth non-aromatic ring to the flavin isoalloxazine group.

17 annealing step was necessary to perfect the isoalloxazine helix and expel the C60 moiety away from t

18 i-pi overlap between the graphene sidewalls, isoalloxazine helix, and the C60 cage that facilitates S

19 hat brings the C60 in pi-pi overlap with the isoalloxazine helix.

20 terface which enable specific binding of the isoalloxazine heterocycle of riboflavin and its two cofa

21 sulfate (SDS) overcomes this strong nanotube/isoalloxazine interaction and restores the FMN into exte

22 atural substrate is bound to the enzyme, the isoalloxazine is in the correct position (the "in" confo

23 The isoalloxazine is observed to move between these conforma

24 number of spectral features associated with isoalloxazine modes in this pH range are evidence for a

25 reactant, the rings of the nicotinamide and isoalloxazine moieties are stacked roughly parallel to e

26 onalized flavin (FC60), composed of PCBM and isoalloxazine moieties attached on either ends of a line

27 rs to be due to hydrogen bonding between the isoalloxazine moiety and the protein.

28 avodoxins catalyze redox reactions using the isoalloxazine moiety of the flavin mononucleotide (FMN)

29 otinamide moiety into close contact with the isoalloxazine moiety of the flavin.

30 OH) is described that contains the tricyclic isoalloxazine moiety, the C-4a-hydroperoxide functionali

31 es belonging to certain carbons of the FMN's isoalloxazine moiety.

32 It is therefore concluded that isoalloxazine movement is required for pyridine nucleoti

33 likely reflects the interaction between the isoalloxazine N1 of the orotate-reducing flavin and Lys

34 of the oxidized cofactor can be observed, an isoalloxazine N5-iminium adduct and a N5 secondary ketim

35 aptamers recognize with high specificity the isoalloxazine nucleus of FAD but do not distinguish FAD

36 droxybenzoate (2,4-diOHB) indicated that the isoalloxazine of the artificial flavin adopts the more s

37 -lived charge-transfer complex involving the isoalloxazine of the FAD cofactor.

38 showed that reduction of FAD occurs when the isoalloxazine of the FAD moves to the surface of the pro

39 s of reduction of the enzyme require (a) the isoalloxazine of the flavin be held by the protein in a

40 n of a narrow tunnel leading directly to the isoalloxazine portion of the FAD prosthetic group.

41 o arise from the ring current field from the isoalloxazine portion of the flavin cofactor.

42 Upon blue-light excitation, the isoalloxazine ring (ISO) may undergo an ultrafast reduct

43 cofactor, including a 22 degrees bend of the isoalloxazine ring along the N(5)-N(10) axis, crankshaft

44 ich makes van der Waals contact with the FAD isoalloxazine ring and also hydrogen-bonds to the ribity

45 n bonding and pi-pi interactions between the isoalloxazine ring and either the nicotinamide ring or T

46 The FMN is bound with hydrogen bonds to the isoalloxazine ring and electrostatic interactions with t

47 trix shield the reactive C4a position of the isoalloxazine ring and force the tricycle into an atypic

48 significant overlap between the intercalated isoalloxazine ring and its adjacent base-triple platform

49 gen bond is formed between the N5 of the FAD isoalloxazine ring and the hydroxyl side chain of alpha

50 owing that the environments for the flavin's isoalloxazine ring are not identical in the two phases.

51 exes are consistent with modification of the isoalloxazine ring at position N5.

52 . vulgaris flavodoxin that are necessary for isoalloxazine ring binding.

53 owever, the FADH- structure reveals that the isoalloxazine ring buckles in the opposite sense, this a

54 y bound to RNA through interactions with the isoalloxazine ring chromophore and direct and Mg(2+)-med

55 tional rearrangements of the protein and the isoalloxazine ring during catalysis.

56 ormational rearrangements of the protein and isoalloxazine ring during catalysis.

57 elix and to the active-site entrance; an FAD isoalloxazine ring exposed to solvent; and a large and a

58 from solution is bound, the insertion of the isoalloxazine ring first.

59 r-401 and Phe-485 in phiLOV sandwich the FMN isoalloxazine ring from both sides, whereas Ser-390 anch

60 The isoalloxazine ring has a butterfly angle of 25 degrees ,

61 Phe(1395) stacks parallel to the FAD isoalloxazine ring in neuronal nitric-oxide synthase (nN

62 ent and placement with respect to the flavin isoalloxazine ring in the active site of rhNQO1; a quali

63 alpha beta is due to solvent exposure of the isoalloxazine ring in the beta 2 active site.

64 , are achieved by moving the position of the isoalloxazine ring in the protein structure.

65 The isoalloxazine ring is coordinated by an unusual cis-Ala-

66 In this model, the binding of the flavin isoalloxazine ring is dependent on the presence of a pho

67 The Raman data show that the isoalloxazine ring is predominantly "out" for Tyr222Phe.

68 The flavin isoalloxazine ring is sandwiched between two tryptophan

69 Its His-289 residue in the re-side of the isoalloxazine ring is within hydrogen bonding distance w

70 The spectra consist of a rich assortment of isoalloxazine ring modes whose normal mode origin can be

71 gen at C6 of DHO is transferred to N5 of the isoalloxazine ring of an enzyme-bound FMN prosthetic gro

72 isting of the substrate (cyclohexanone), the isoalloxazine ring of C4a-peroxyflavin, the side chain o

73 residue in the FNR module of NOS shields the isoalloxazine ring of FAD and is known to regulate NADPH

74 e at reaction distance to the N5 atom of the isoalloxazine ring of FAD and the hydroxyl group of Tyr(

75 droxyl group of serine or threonine with the isoalloxazine ring of FAD and with the amino acids in it

76 In the model, Pdx is docked above the isoalloxazine ring of FAD of Pdr with the distance betwe

77 de ring of NADPH, which is juxtaposed to the isoalloxazine ring of FAD to facilitate hydride transfer

78 t that is in contact with the re face of the isoalloxazine ring of FAD when the structure of PchF is

79 oned directly above and in parallel with the isoalloxazine ring of FAD, and mass spectrometry extende

80 zolyl ring of 3FMTDZ was positioned over the isoalloxazine ring of FAD, whereas that of HETDZ had the

81 charge and 4-5 masculine bend in the reduced isoalloxazine ring of FAD, which resulted in a new mode

82 1 forms pi-pi stacking interactions with the isoalloxazine ring of FAD.

83 n selected because of their proximity to the isoalloxazine ring of FAD.

84 n a conserved phenylalanine, Phe223, and the isoalloxazine ring of FAD.

85 at resveratrol molecule in parallel with the isoalloxazine ring of FAD.

86 difference in active site residues near the isoalloxazine ring of FAD: Val402 in EcPutA is substitut

87 active site and would appear to require the isoalloxazine ring of FADH- to buckle in a particular wa

88 stabilizes developing negative charge on the isoalloxazine ring of flavin mononucleotide during hydri

89 a base-triple on complex formation with the isoalloxazine ring of FMN intercalating into the helix b

90 rogen bonding of the uracil like edge of the isoalloxazine ring of FMN to the Hoogsteen edge of an ad

91 The isoalloxazine ring of FMN was shown buried within a narr

92 the peptide Gly57-Asp58, in a bend near the isoalloxazine ring of FMN, is correlated with the oxidat

93 The isoalloxazine ring of the bound FAD is more buried in th

94 Since the isoalloxazine ring of the chromophore is unable to under

95 th the structure of rat CPR, is close to the isoalloxazine ring of the enzyme-bound FAD.

96 irpin conformation and is wedged between the isoalloxazine ring of the FAD and the side chain of Phe2

97 beta bond of the thioester substrate and the isoalloxazine ring of the FAD are located, is larger in

98 utilizing a pi-stacking interaction with the isoalloxazine ring of the FAD cofactor.

99 tion of FADH(-) in photolyases, in which the isoalloxazine ring of the flavin and the adenine are in

100 gen at C6 of DHO is transferred to N5 of the isoalloxazine ring of the flavin as a hydride.

101 jority of the critical interactions with the isoalloxazine ring of the flavin mononucleotide (FMN) co

102 in favorable pi-sigma interactions with the isoalloxazine ring of the flavin to help stabilize forma

103 77), which stacks against the re-face of the isoalloxazine ring of the flavin.

104 at presents a CxxC disulfide proximal to the isoalloxazine ring of the flavin.

105 ect contact with the re or inner face of the isoalloxazine ring of the FMN cofactor.

106 relative position of a peptide loop and the isoalloxazine ring of the FMN is slightly different in t

107 matic substitution, diradical formation, and isoalloxazine ring opening have been proposed.

108 es flank the flavin, which is bound with its isoalloxazine ring perpendicular to a five-stranded beta

109 cated in the antigen-combining site with its isoalloxazine ring stacked between the parallel aromatic

110 hat the spin density distribution within the isoalloxazine ring system depends critically on the natu

111 te hydroxylase (PHBH) have revealed that the isoalloxazine ring system of FAD is capable of adopting

112 The isoalloxazine ring system of one conformation (the "out"

113 , Tyr-466, and Ser-468) in a pocket near the isoalloxazine ring system of the FAD co-factor.

114 the nicotinamide base stacks directly on the isoalloxazine ring system of the FAD.

115 ble loop (loop III) above the si-face of the isoalloxazine ring that changes position depending on th

116 antiomer cannot approach close enough to the isoalloxazine ring to form a flavin adduct, but can be f

117 The binding of the flavin isoalloxazine ring to its subsite is dependent on the pr

118 either adduct formation or reduction of the isoalloxazine ring to the neutral semiquinone, both of w

119 The 8-position of the FAD isoalloxazine ring was chosen for modification because i

120 The 8-position of the FAD isoalloxazine ring was chosen for modifications, because

121 dithiol substrates of these oxidases to the isoalloxazine ring where the reaction with molecular oxy

122 amide moiety of NADP(+) lies against the FAD isoalloxazine ring with a tilt of approximately 30 degre

123 through conformational rearrangements of the isoalloxazine ring within the protein structure.

124 yl of the ribityl chain of FAD and N1 of the isoalloxazine ring, and between alpha H286 and the C2-ca

125 evere (35 degrees ) butterfly bending of the isoalloxazine ring, and disruption of an electrostatic n

126 hat stacks against the si-face of the flavin isoalloxazine ring, and P92, the second residue in the m

127 the methyl group of Thr-394 "crowds" the FMN isoalloxazine ring, Leu-470 triggers side chain "flippin

128 alpha H286 and the C2-carbonyl oxygen of the isoalloxazine ring, may play a role in the stabilization

129 the plane of the FMN via pi-overlap with the isoalloxazine ring, penetrating deep into the groove, wi

130 ension concurrent with a 5 A movement of the isoalloxazine ring, positioning the flavin ring adjacent

131 ing into the sulfur-reducing side of the FAD isoalloxazine ring, suggesting how this enzyme class may

132 d provides several interactions with the FMN isoalloxazine ring, was targeted in this study.

133 vement of the side chain of Trp60 out of the isoalloxazine ring-binding site and other associated con

134 ing subsite or the loops that constitute the isoalloxazine ring-binding site.

135 ate-induced conformational change within the isoalloxazine ring-binding subsite.

136 apoflavodoxin that is capable of binding the isoalloxazine ring.

137 re believed to bind to it mainly through the isoalloxazine ring.

138 ormational change in the vicinity of the FAD isoalloxazine ring.

139 ition to directly attack the C4a atom of the isoalloxazine ring.

140 degrees along the N5-N10 axis of the flavin isoalloxazine ring.

141 thyl group beta-protons at position 8 of the isoalloxazine ring.

142 e dynamics and the planarity of their flavin isoalloxazine ring.

143 otates to become almost perpendicular to the isoalloxazine ring.

144 in terms of the difference at C8alpha of the isoalloxazine ring.

145 and BrdUMP in a closed conformation near the isoalloxazine ring.

146 epting a hydrogen bond from the H(N5) of the isoalloxazine ring.

147 synthase, Phe1395 is positioned over the FAD isoalloxazine ring.

148 conformational changes are apparent for the isoalloxazine ring; the three-ring system exhibits more

149 -FPR place their respective nicotinamide and isoalloxazine rings 15 A apart and separated by residues

150 Two stacked isoalloxazine rings and nicotinamide/isoalloxazine rings

151 The two flavin isoalloxazine rings are juxtaposed, with the closest dis

152 The stacked nicotinamide:isoalloxazine rings in TftC and sequential reaction kine

153 The distance between the two isoalloxazine rings is 18 A.

154 d be necessary to place the nicotinamide and isoalloxazine rings parallel and adjacent to one another

155 based on the interaction of the adenine and isoalloxazine rings that can be tailored by the "solvati

156 Moreover, the known ability of isoalloxazine rings to act as metal chelators, along wit

157 stacked isoalloxazine rings and nicotinamide/isoalloxazine rings were at a proper distance for hydrid

158 ide (FMN) and apo-flavodoxin is dominated by isoalloxazine-stacking interactions and 5'-phosphate hyd

159 S-C1(=O)-C2 plane of the substrate with the isoalloxazine substantially alter rates of the reductive

160 te to provide multiple interactions with the isoalloxazine system of FMN that are usually provided by

161 ove into position to form a complex with the isoalloxazine that is competent for hydride transfer and

162 to the stretch of the 4C&dbd;O group of the isoalloxazine; the relatively narrow profile of this fea

163 actopyranose binds at the re face of the FAD isoalloxazine with the anomeric carbon atom poised for n

164 early parallel to the middle ring of the FAD isoalloxazine, with the inhibitor C5 atom 3.3 A from the