ライフサイエンスコーパス: isoaspartate

1 , indicated that cruciferin subunits contain isoaspartate.

2 The link between isoaspartate accumulation and the neurological abnormali

3 ut) mice exhibit greatly increased levels of isoaspartate and typically succumb to fatal epileptic se

4 thod can accurately detect 5 pmol or less of isoaspartate and works with tryptic digests as well as i

5 tein, suggesting that it contains a level of isoaspartate at least 50 times greater than that of the

6 Subsequently, the existence and site of isoaspartate can be confirmed by electron transfer disso

7 une antigens, of particular interest because isoaspartate can greatly enhance the antigenicity of sel

8 n neurons, accumulated exceptional levels of isoaspartate: collapsin response mediator protein 2 (CRM

9 Moreover, the distribution of isoaspartate-containing proteins in E. coli differed dra

10 particular allows the affinity enrichment of isoaspartate-containing proteins.

11 lived, has been hindered by large amounts of isoaspartate-containing storage proteins.

12 a substantial and consistent increase in the isoaspartate content of tubulin was observed.

13 The enzyme protein L-isoaspartate (D-aspartate) O-methyltransferase (E.C. 2.1

14 mapping of the gene for the murine protein-L-isoaspartate (D-aspartate) O-methyltransferase (EC 2.1.1

15 ave demonstrated that mice lacking protein L-isoaspartate (D-aspartate) O-methyltransferase (Pcmt1-/-

16 Protein-L-isoaspartate (D-aspartate) O-methyltransferases (EC 2.1.

17 Protein L-isoaspartate (D-aspartate) O-methyltransferases (MTs; EC

18 ly conserved cytosolic enzyme, the protein L-isoaspartate(D-aspartate) O-methyltransferase (EC 2.1.1.

19 The widely distributed protein-L-isoaspartate(D-aspartate) O-methyltransferase (PIMT; EC

20 e embryonic stem cells to generate protein L-isoaspartate(D-aspartate) O-methyltransferase-deficient

21 Protein l-isoaspartate-(d-aspartate) O-methyltransferases (EC ), p

22 ay shed important new light on mechanisms of isoaspartate formation in cells and the molecular pathol

23 s suggests that tubulin constantly undergoes isoaspartate formation in vivo, but that the levels are

24 Isoaspartate formation is a ubiquitous post-translation

25 isoaspartate formation occurred in parallel with, but wa

26 The near stoichiometric levels of isoaspartate in S11, estimated at 0.5 mol of isoaspartat

27 The formation of isoaspartate inserts a methylene group into the protein

28 rtate methyltransferase selectively converts isoaspartates into the corresponding methyl esters.

29 rium between Snn and its hydrolysis products isoaspartate (isoAsp) and aspartate.

30 tion in these MAbs leads to formation of the isoaspartate (IsoAsp) and the cyclic imide (Asu) variant

31 Aspartate-to-isoaspartate isomerization in proteins occurs in cells b

32 OGT, enzymes that may catalyze aspartate to isoaspartate isomerization include PARPs, enzymes known

33 soaspartyl peptide, the relationship between isoaspartate levels and S-adenosyl-l-homocysteine produc

34 the possibility that the rat enzyme reduces isoaspartate levels in E. coli proteins, a result predic

35 sensitive HPLC-based method for quantitating isoaspartate levels in peptides and proteins is describe

36 Isoaspartate levels in synapsin from the knock-out mice

37 expressing rat PIMT had significantly lower isoaspartate levels than control cells, especially in st

38 Isoaspartate levels were estimated by the transfer of ra

39 (At3g48330 and At5g50240) encoding protein-l-isoaspartate methyltransferase (EC 2.1.1.77; PIMT), an e

40 The enzyme protein L-isoaspartate methyltransferase (PIMT), present in many b

41 is first specifically methylated by protein isoaspartate methyltransferase (PIMT, EC 2.1.1.77) to th

42 In the initial step, protein isoaspartate methyltransferase selectively converts isoa

43 dation and by isoAsp detection using protein isoaspartate methyltransferase.

44 m knock-out mice contains 0.9 +/- 0.3 mol of isoaspartate/mol of synapsin, whereas the levels in wild

45 Protein L-isoaspartate O-methyltransferase (PIMT) is postulated to

46 In almost all organisms, protein L-isoaspartate O-methyltransferase (PIMT, EC2.1.1.77) reco

47 Protein l-isoaspartate O-methyltransferase activity, a repair enzy

48 Isomerization of aspartate to isoaspartate occurs spontaneously in proteins, causes ch

49 ematic and comprehensive characterization of isoaspartate, particularly in complex systems.

50 isoaspartate in S11, estimated at 0.5 mol of isoaspartate per mol of S11, suggests that this unusual

51 Further study of the isoaspartate-prone proteins identified here may help elu

52 These isoaspartate-prone proteins represent a wide range of ce

53 t a chemo-enzymatic detection method for the isoaspartate protein, which in particular allows the aff

54 ethyltransferase in neurons and suggest that isoaspartate-related alterations in the function of pres

55 notype results from the cumulative effect of isoaspartate-related damage to a number of the neuron-ri

56 ic-phase cells, nearly all of the detectable isoaspartate resided in a single 14-kDa protein which we

57 ersion of asparagine into both aspartate and isoaspartate residues.

58 duce mature proteins capable of converting l-isoaspartate to l-aspartate in small peptide substrates.

59 ts, and animals, catalyzes the conversion of isoaspartate to normal alpha-linked aspartyl bonds and i

60 lecular mass range of 66 to 14 kDa contained isoaspartate, whereas in logarithmic-phase cells, nearly