ライフサイエンスコーパス: isobaric

1 se after Kdo(2)-lipid A exposure, whereas an isobaric 50:1-TAG containing 16:1 did not change relativ

2 report the synthesis and characterization of isobaric aldehyde reactive tags (iARTs) for glycan quant

3 vel isobaric reagent, named deuterium ((2)H) isobaric amine-reactive tag (DiART), for quantitative pr

4 des and proteins including discrimination of isobaric amino acid residues and site determination of p

5 In particular, d ions help to identify isobaric amino acid residues present near the N-terminal

6 likely be used to differentiate between the isobaric amino acids Mdha and Dhb in candidate cyanotoxi

7 ents a trapped water molecule rather than an isobaric ammonium ion.

8 oubly sodiated adducts ([M + 2Na - H](+)) of isobaric and coeluting steroid metabolites allowed their

9 f measured sphingolipids by resolving nearly isobaric and isobaric species; this is accomplished by a

10 de liquid chromatography-like separations of isobaric and isomeric compounds in a fraction of the tim

11 ics workflow is its ability to separate many isobaric and isomeric lipids that by standard shotgun li

12 ilitate correct lipid assignments among many isobaric and isomeric species independent of the resolut

13 fy and image both abundant and low-abundance isobaric and isomeric species within each m/z window.

14 NAs separated into three clusters indicating isobaric and isomeric species.

15 hain kinase (MLCK) inhibitor ML-7 under both isobaric and isometric conditions.

16 jor histone marks and discrimination between isobaric and nearly isobaric PTMs such as trimethylation

17 tion is the effective chemical separation of isobaric and polyatomic interferences prior to sample in

18 om hexagonal ice to HDA and then to VHDA via isobaric annealing).

19 a 3.0 performs label-free quantification and isobaric chemical labeling quantification in addition to

20 Multiplexed quantitation via isobaric chemical tags (e.g., tandem mass tags (TMT) and

21 Multiplex quantitation via isobaric chemical tags (e.g., TMT and iTRAQ) provides an

22 s analyses is complicated by the presence of isobaric coeluting peptides, as they generate the same e

23 heir modular designs, an unlimited number of isobaric combinations of CIT reagents are, in principle,

24 strate significant reduction of isomeric and isobaric complexity for Suwannee River fulvic acid (SRFA

25 Isobaric compositions were well-delineated using TSA by

26 formance of neural computing predictions for isobaric compound retention time is also discussed.

27 chemical assignment owing to the presence of isobaric compounds and, depending on mass resolution, io

28 ry (FTMS) allows to distinguish between most isobaric compounds as well as to unambiguously determine

29 dem mass spectrometer distinguishes ACh from isobaric compounds in the R2 neuron and demonstrates the

30 This allowed distinguishing coeluted isobaric compounds without requiring additional separati

31 of interfering compounds (i.e., isomers and isobaric compounds) is also taken into account.

32 in metabolite identification, especially for isobaric compounds.

33 t is limited in its capacity to discriminate isobaric compounds.

34 a valuable tool toward the identification of isobaric compounds.

35 e) was subjected to processing at isothermal-isobaric conditions at temperatures from 30 to 80 degree

36 Online digestion was performed under isobaric conditions with continuous solvent flow, in con

37 This elasticity leads to quasi-isobaric conditions within the confined region.

38 ssure, for both isothermal decompression and isobaric cooling paths.

39 publications, mainly due to the presence of isobaric cross-linked species, an incomplete fragmentati

40 ly in the cationic tag portion, which allows isobaric derivatives to be distinguished by tandem mass

41 iminated between microcystins containing the isobaric [Dhb(7)]- and [Mdha(7)]-groups.

42 TAGs of the same molecular weight that yield isobaric diacylglycerol-like product ions.

43 dy at Leuven University Hospital with graded isobaric distentions before and after a liquid meal.

44 of saccharides and other parameters such as isobaric expansion coefficients, interaction coefficient

45 The isobaric forms occur only during the bleaching step of t

46 ntent and detected, for the first time, some isobaric forms of natural (+)-pinoresinol and (+)-1-acet

47 ble to monitor the exchange progress for two isobaric fragments originating from two or more differen

48 -orbital methods for several of their common isobaric fragments.

49 is and the resolving power needed to resolve isobaric fragments.

50 GlcCer and GlcSph from the far more abundant isobaric galactosyl epimers naturally occurring in white

51 ere chromatographically separated from their isobaric galactosylceramide (GalCer) counterparts using

52 A/X ions), thus affording differentiation of isobaric gangliosides.

53 Using isobaric gas-tight samplers, we collected discrete sampl

54 Moreover, we show here that isobaric glycans could be distinguished by structure spe

55 iquid water's isothermal compressibility and isobaric heat capacity, and the magnitude of its thermal

56 We designed and created isobaric hypoxic chambers able to maintain pregnant shee

57 e transition to air-breathing at birth using isobaric hypoxic chambers without alterations to materna

58 Isobaric interference from O(2)(+) is minimized by emplo

59 nanodiscs in a controllable way, eliminating isobaric interference from the lipids.

60 hes using ICP methods for chlorine isotopes, isobaric interference of the (36)ArH dimer with (37)Cl w

61 e is ensuring removal of barium to eliminate isobaric interferences arising from (135)Ba and (137)Ba.

62 Major isobaric interferences for the analyzed elements were re

63 curate detection is the effective removal of isobaric interferences from (135)Ba and (137)Ba prior to

64 n unspiked urine (>250%) by the reduction of isobaric interferences from the matrix.

65 abundances below 1 ppm and is not limited by isobaric interferences such as (235)UH(+) during measure

66 ion accurate mass analysis by elimination of isobaric interferences, a fundamentally different concep

67 electron energy of 35 V for Ne to eliminate isobaric interferences, and a higher electron energy for

68 13%, n = 117) with minimal ion suppression, isobaric interferences, and sample carry-over (<1%).

69 After correction for isobaric interferences, in particular (176)Yb, we were a

70 tiple charge state distributions can lead to isobaric interferences, mass spectral congestion, and am

71 atrix interferences, spectral interferences, isobaric interferences, polyatomic interferences, and ab

72 stage was employed to selectively remove the isobaric interferences.

73 logs, as proxies to these radioisotopes, and isobaric interferents were introduced to DMS.

74 ccuracy adequate for separating the ion from isobaric interfering ions.

75 challenges (i.e., competitive ionization and isobaric ion overlap) were explored and analyzed with LC

76 f each element, which requires deconvoluting isobaric ion signals at 4 m/z (O(4+) and C(3+)) and at 8

77 This demonstrates that isobaric ions are present during MALDI-LIT-MS analyses o

78 monly used positive ion spectra that contain isobaric ions formed by losses from different regions of

79 rmation (Delta(f)H) obtained by QCC for some isobaric ions from the electron ionization (EI)-MS mass

80 Due to the large number of isobaric ions in the precursor isolation ranges, large n

81 id atomic analyses especially in the case of isobaric ions that cannot be distinguished by MS.

82 in these data are the removal of interfering isobaric ions within the images of m/z 811.4 and also of

83 effects, difficulty in separating isomer and isobaric ions, and long analysis times.

84 pancies are explained by the presence of two isobaric ions, one due to (13)C and the other due to the

85 matically resolving overlapping isotopes and isobaric ions.

86 tion of the ROMIAC facilitates separation of isobaric isomeric peptides.

87 and L enantiomers, a methylated amino acid, isobaric isomers and short peptides.

88 a-branch or meromycolate chain, and up to 10 isobaric isomers were identified for some minor ions.

89 in laboratory studies and the occurrence of isobaric isomers, a complete assignment for individual M

90 using molecular dynamics simulations in the isobaric-isothermal ensemble, confirming our thermodynam

91 Using isobaric-isothermal replica-exchange molecular dynamics

92 Given that isobaric isotopomers cannot be separated chromatographic

93 a method that purposely uses high amounts of isobaric label (levels that induce denaturation) to enha

94 , namely, QMIX (Quantitation of Multiplexed, Isobaric-labeled cross (X)-linked peptides) by integrati

95 blocking peptide amino groups from isotopic/isobaric labeling and changing peptide charge states, re

96 Combinatorial Isobaric Mass Tags (CMTs), an isobaric labeling architecture with the unique ability t

97 process includes abundant protein depletion, isobaric labeling at the peptide level for multiplexed r

98 of the collection, we have used multiplexed isobaric labeling for quantitative proteomics to derive

99 proteome and phosphoproteome via multiplexed isobaric labeling proteomics technology, computational p

100 CMT with respect to a commercially available isobaric labeling reagent with preserved quantitative ac

101 integrating MS-cleavable cross-linkers with isobaric labeling reagents.

102 Isobaric labeling strategies allow mass spectrometry-bas

103 Isobaric labeling strategies for mass spectrometry-based

104 Isobaric labeling strategies, such as isobaric tags for

105 The use of tandem mass tags (TMT) as an isobaric labeling strategy is a powerful method for quan

106 re of human and yeast proteins) in a sixplex isobaric labeling system to fully document the interfere

107 y on-resin protein digestion and multiplexed isobaric labeling to facilitate liquid chromatography-ta

108 Here, protein-level isobaric labeling was explored as an alternative strateg

109 s spectrometry, relative quantification with isobaric labeling, iTRAQ(TM), was used to compare the re

110 New features for analysis of isobaric labeling, such as Tandem Mass Tag (TMT) or Isob

111 Using isobaric labeling-based quantitative proteomics, we simu

112 gnitive decline was analyzed using multiplex isobaric labeling.

113 Here we use isobaric-labeling multiplexed proteomics to analyze prot

114 ned by quantitative comparison using TMT(TM) isobaric labelling.

115 The service supports queries where isobaric leucine and isoleucine are treated equivalent,

116 The detection of new isobaric lignans is suggested to reveal frauds in commer

117 t prevalent glycans were a mixture of novel, isobaric, linear, and branched forms of a glucuronyl cor

118 by separating vinblastine from an endogenous isobaric lipid.

119 d us to generate ion images of low-abundance isobaric lipids.

120 ctrometry is particularly challenging due to isobaric mass between the isomeric forms.

121 are rarely available or suffer from various isobaric mass interferences.

122 ent was used to eliminate the possibility of isobaric mass overlap of these species with the precurso

123 se species to be readily differentiated from isobaric mass plasmanyl (alkyl-ether) containing lipids.

124 Isobaric mass tag-based quantitative proteomics strategi

125 Isobaric mass tag-based quantitative proteomics strategi

126 Using isobaric mass tagging experiments, mass spectrometry-bas

127 We present Combinatorial Isobaric Mass Tags (CMTs), an isobaric labeling architec

128 bottom-up, shotgun proteomics technologies (isobaric mass tags for relative and absolute quantificat

129 In view of the success of isobaric mass tags in quantitative bottom-up proteomics,

130 rk we evaluated the performance of the TMT10 isobaric mass tags on the Q Exactive Orbitrap mass spect

131 chromatin, extended multiplexing capacity of isobaric mass tags, state-of-the-art mass spectrometry m

132 itative, bottom-up proteomics analysis using isobaric mass tags.

133 were compared with wild-type cells using an isobaric mass-tag quantitative proteomics approach to id

134 00 also aided in the differentiation of near isobaric metabolites, but higher MS resolution reduced t

135 ing power for polar molecules including many isobaric metabolites.

136 rement (HR/AM) capabilities to differentiate isobaric metabolites.

137 se lipids are complicated by the presence of isobaric molecular ions shared by these lipid classes an

138 ied for quantitative identification of exact isobaric molecules in their mixtures, which is one of th

139 t range, but cannot differentiate isomers or isobaric molecules, using the conventional quadrupole ma

140 a by exhaustively enumerating all single non-isobaric mutations for every residue in a protein databa

141 enges to mass spectral analysis due to their isobaric nature and their lability in the gas phase, as

142 The isobaric nature of trimethylation and acetylation (42.04

143 e subjected to HPP (130-530 MPa) under quasi-isobaric non-isothermal conditions (15 min; 0, 4.6 and 9

144 paration inlet for MS, capable of separating isobaric oligosaccharide isomers prior to MS detection a

145 (IMS) has been shown to effectively resolve isobaric oligosaccharides, but the utility of IMS to obt

146 Isobaric or near-isobaric precursor ions with different

147 gnal-to-noise ratio due to the separation of isobaric or other interfering ionic species.

148 educing the cost and effort for synthesis of isobaric pairs.

149 arent specific volumes (varphiVsp), standard isobaric partial molar expansibility (varphiE(0)) and it

150 distinct reactivity to separate isomeric and isobaric PC and PE cations present in mass spectra of li

151 e cleanup method which aimed to remove CTTs, isobaric PCBs, and sample matrix.

152 g the optimized AP-DTIMS-Orbitrap MS system, isobaric peptide and sugar isomers were successfully res

153 ic fine structure information of a number of isobaric peptide pairs, including a pair of peptides ori

154 diDO-IPTL approach combines two advances in isobaric peptide terminal labeling (IPTL) methodology: f

155 Isobaric peptide termini labeling (IPTL) is based on lab

156 mine our data set and quantify 16 additional isobaric peptides commonly not targeted during DDA exper

157 data remining to later identify and quantify isobaric peptides unknown at the time of the LC-MS exper

158 h complementary isotopic labels resulting in isobaric peptides.

159 d targeted MS/MS scans, the latter targeting isobaric peptides.

160 eparation and identification of isomeric and isobaric phospholipids that are difficult to separate in

161 ing isobaric phosphopeptides, with up to two isobaric phosphopeptides sequenced from a single mass sp

162 spectra allow facile sequencing of coeluting isobaric phosphopeptides, with up to two isobaric phosph

163 ision cross sections), we further resolved 5 isobaric pigments, not resolved by UHPLC-UV-MS(E) alone.

164 PLTX, in 25% of them only OVTX-a, -d, -e and isobaric PLTX were present, while 4% produced only OVTX-

165 strains contained OVTX-a to -e, OVTX-g, and isobaric PLTX, in 25% of them only OVTX-a, -d, -e and is

166 er allowed for the identification of complex isobaric polar lipids such as NeuAc- and NeuGc-containin

167 Given the high column efficiency, isobaric positional isomers of a single sialic acid on b

168 Isobaric or near-isobaric precursor ions with different chromatographic p

169 sitional isomers from spectra of mixtures of isobaric protein forms.

170 Isobaric protein-level labeling gave comparable identifi

171 The compatibility of isobaric protein-level labeling with lectin-based glycop

172 d discrimination between isobaric and nearly isobaric PTMs such as trimethylation and acetylation.

173 96 Da, which enables the distinction between isobaric pyro- and diphosphorylated peptides.

174 per demonstrates the applications of a novel isobaric reagent, named deuterium ((2)H) isobaric amine-

175 ediately expanding the throughput of the TMT isobaric reagents from 6-plex to 8-plex.

176 m for increasing the multiplexing density of isobaric reagents.

177 tely measure the relative intensity of these isobaric reporter ions, we demonstrate that we are able

178 sequencing of mAbs is the discrimination of isobaric residues (Xle): leucine (Leu) and isoleucine (I

179 gned to facilitate unequivocal assignment of isobaric residues by MALDI-TOF MS analysis.

180 When compositions of an isobaric series of molecular formulae consisting of C, H

181 rters by intervals of 6 mDa yields a 12-plex isobaric set that preserves the synthetic simplicity and

182 Specifically, isobaric silver ion adducts of C29 alkane (m/z 515.3741)

183 2, enabling separation and identification of isobaric species (compounds with the same nominal mass b

184 f chemical noise and separation of coeluting isobaric species across the entire analytical space.

185 of a multifaceted approach for the study of isobaric species and elucidates why previous MDA studies

186 -MS workflow and maximized the separation of isobaric species and the signal-to-noise ratio in direct

187 It was possible to identify isobaric species differing by their fatty acid chains th

188 slead conclusions, especially in cases where isobaric species exhibit different distributions within

189 andem MS is necessary to distinguish between isobaric species for selective imaging of carbohydrates

190 nd previous investigations revealed multiple isobaric species having molecular weights at virtually e

191 but are limited by the inability to resolve isobaric species such as linkage and positional isomers.

192 minor lipid A species consisted of multiple isobaric species with acyl chains of various lengths.

193 PE and modified PC species that would yield isobaric species with unlabeled diazomethane.

194 differentiate between structural isomers and isobaric species.

195 feature detection in part by time-resolving isobaric species.

196 onvolution of mass spectra from isomeric and isobaric species.

197 enerate images of specific product ions from isobaric species.

198 e (BMP) lipids, and to distinguish them from isobaric species.

199 id tumor sample with reduced interference of isobaric species.

200 hingolipids by resolving nearly isobaric and isobaric species; this is accomplished by a use of (i) a

201 r difficult to separate structurally related isobaric species; thus, it provides additional selectivi

202 eloped leaves using stable isotope labeling (isobaric stable isotope labeling and isotope-coded affin

203 Even mass isobaric structures that differ only in the branching po

204 porter ions from quaternary amine containing isobaric tag for glycan (QUANTITY) isobaric tags are use

205 resent QUANTITY (Quaternary Amine Containing Isobaric Tag for Glycan), a quantitative approach that c

206 Analyses using isobaric tag for relative and absolute quantification (i

207 Isobaric tag for relative and absolute quantification (i

208 we combined (i) the power of unbiased iTRAQ (isobaric tag for relative and absolute quantification) m

209 on-centric (e.g. isotope coded affinity tag, isobaric tag for relative and absolute quantification, e

210 Isobaric tag for relative and absolute quantitation (iTR

211 An isobaric tag for relative and absolute quantitation (iTR

212 Proteomic isobaric tag for relative and absolute quantitation (iTR

213 e quantitative phosphoproteomic methodology, isobaric tag for relative and absolute quantitation (iTR

214 LC MALDI MS and isobaric tag for relative and absolute quantitation tech

215 frozen and OCT-embedded tissues using iTRAQ (isobaric tag for relative and absolute quantitation) lab

216 treating cocaine addition, an 8-plex iTRAQ (isobaric tag for relative and absolute quantitation) pro

217 Digested samples were treated with iTRAQ (isobaric tag for relative and absolute quantitation).

218 ntitative proteome analysis was performed by isobaric tag for relative and absolute quantitation-labe

219 In particular, isobaric tag labeled glycopeptides after C18 desalting c

220 While peptide-level labeling using isobaric tag reagents has been widely applied for quanti

221 ethod, QuantMode, which improves accuracy of isobaric tag-based quantification by alleviating the per

222 Here we used isobaric tag-based quantitative mass spectrometry to det

223 We report here an isobaric-tagged isotope dilution mass spectrometry metho

224 large-scale analysis of both unmodified and isobaric-tagged peptides.

225 hat IRMPD is compatible with the analysis of isobaric-tagged peptides.

226 ses combined precursor isotopic labeling and isobaric tagging (cPILOT) to increase the multiplexing c

227 oach combining tandem mass-spectrometry with isobaric tagging (iTRAQ) and multiple database searching

228 We used quantitative isobaric tagging (iTRAQ) proteomics to determine the L.

229 yzed proximity labeling can be combined with isobaric tagging and mass spectrometry to enable quantit

230 bining high-mass-accuracy mass spectrometry, isobaric tagging and software for multiplexed, large-sca

231 Isobaric tagging enables the acquisition of highly multi

232 Here, we describe a biotin switch isobaric tagging for relative and absolute quantificatio

233 We performed isobaric tagging for relative and absolute quantificatio

234 An isobaric tagging for relative and absolute quantificatio

235 ts was subjected to proteomic analysis using isobaric tagging for relative quantification.

236 monstrated which employ isotopic labeling or isobaric tagging in order to quantify differences in the

237 Amine-reactive isobaric tagging reagents such as iTRAQ (isobaric tags f

238 standard peptides and digested proteins with isobaric tagging reagents were studied using a mixture o

239 fication was carried out using eight channel isobaric tagging with iTRAQ reagent, and proteins were i

240 nd the multiplexing approach which relies on isobaric tagging.

241 quantitative proteomics experiments that use isobaric tagging.

242 e, multiplexed quantitative proteomics using isobaric tagging.

243 otein quantification, referred to as Caltech isobaric tags (CITs), which offer several advantages in

244 several advantages in comparison with other isobaric tags (e.g., iTRAQ and TMT).

245 Multiplex isobaric tags (e.g., tandem mass tags (TMT) and isobaric

246 vances in multiplexed quantitation utilizing isobaric tags (e.g., TMT and iTRAQ) have the potential t

247 sed, tryptically digested, and labeled using isobaric tags (iTRAQ).

248 ometry-based approach using a combination of isobaric tags and heavy-labelled standard peptides, to c

249 y stable isotope labeling approaches such as isobaric tags and stable isotope labeling of amino acids

250 ontaining isobaric tag for glycan (QUANTITY) isobaric tags are used for quantification of the relativ

251 Isobaric tags for absolute and relative quantitation (iT

252 using novel 10-plex reporter ion-containing isobaric tags for multiplexed amine metabolite analysis.

253 c labeling, such as Tandem Mass Tag (TMT) or Isobaric Tags for Relative and Absolute Quantification (

254 ested with trypsin, labeled using eight-plex isobaric tags for relative and absolute quantification (

255 ercially available tandem mass tags (TMT) or isobaric tags for relative and absolute quantification (

256 ILAC), isotope-coded affinity tag (ICAT), or isobaric tags for relative and absolute quantification (

257 n vivo incubation with in vitro growth using isobaric tags for relative and absolute quantification (

258 baric tags (e.g., tandem mass tags (TMT) and isobaric tags for relative and absolute quantification (

259 itative proteomics analyses by combining the isobaric tags for relative and absolute quantification a

260 By isobaric tags for relative and absolute quantification c

261 The use of isobaric tags for relative and absolute quantification e

262 We confirmed our isobaric tags for relative and absolute quantification f

263 pletion of the most abundant serum proteins, isobaric tags for relative and absolute quantification w

264 (AD) or PDC and their matched controls using isobaric tags for relative and absolute quantification.

265 mical tags (e.g., tandem mass tags (TMT) and isobaric tags for relative and absolute quantitation (iT

266 allotetraploids and their progenitors using isobaric tags for relative and absolute quantitation (iT

267 s response of L. plantarum strains using the isobaric tags for relative and absolute quantitation (iT

268 Isobaric labeling strategies, such as isobaric tags for relative and absolute quantitation (iT

269 eagents serve as attractive alternatives for isobaric tags for relative and absolute quantitation (iT

270 Using a quantitative proteomics method, isobaric tags for relative and absolute quantitation (iT

271 Using isobaric tags for relative and absolute quantitation (iT

272 teins were identified in tomato leaves using isobaric tags for relative and absolute quantitation (iT

273 In this study, we use isobaric tags for relative and absolute quantitation (iT

274 with isotopic labeling strategies employing isobaric tags for relative and absolute quantitation (iT

275 synaptic hippocampal protein content (iTRAQ (Isobaric Tags for Relative and Absolute Quantitation) an

276 ive isobaric tagging reagents such as iTRAQ (isobaric tags for relative and absolute quantitation) ha

277 modifications on the accuracy of the iTRAQ (isobaric tags for relative and absolute quantitation) me

278 In this paper, we describe the use of iTRAQ (isobaric Tags for Relative and Absolute Quantitation) ta

279 quid chromatography-mass spectrometry iTRAQ (isobaric tags for relative and absolute quantitation) te

280 ts association with membranes, an iTRAQ (for isobaric tags for relative and absolute quantitation)- b

281 Isobaric tags for relative and absolute quantitation-bas

282 Isobaric tags for relative and absolute quantitation-lab

283 sed the reproducibility of the AutoTip using isobaric tags for relative N-linked glycan quantificatio

284 of multiple Yn fragment ions that carry the isobaric tags from the inclusion list in the MS/MS/MS sc

285 e target peptide were labeled with the three isobaric tags giving nine 6-plex reactions for 54-plex q

286 Two novel 6-plex isobaric tags were added for a total of three 6-plex exp

287 oxyTMT) reagents are recently commercialized isobaric tags which enable relative quantitation of up t

288 s was achieved by combining amine standards, isobaric tags, and capillary liquid chromatography (LC)

289 We have developed our own multiplex isobaric tags, DiLeu, that feature quantitative performa

290 methods for analysis of mixtures of several isobaric TAGs.

291 ment in protein quantitation precision using isobaric tags.

292 of novel N,N-dimethyl leucine (DiLeu) 4-plex isobaric tandem mass (MS(2)) tagging reagents with high

293 The application of multiplexed isobaric tandem mass tag (TMT) labeling and an LTQ Orbit

294 odology using cerium oxide nanoparticles and isobaric tandem mass tag (TMT) labeling to assess absolu

295 ome of tankyrase double knockout cells using isobaric tandem mass tags reveals targets of degradation

296 t methods: in the bottle (Champenoise) or in isobaric tanks (Charmat method).

297 As the tags are isobaric, the intensity of each peak is the sum of the i

298 In contrast, isobaric TMT labeling with quantification of reporter io

299 hosphorylation and sulfation of tyrosine are isobaric, we used alkaline phosphatase treatment to dist

300 ted to the disulfide scrambled forms and was isobaric with the correctly folded product.