ライフサイエンスコーパス: isochorismate

1 etabolite chorismate into salicylic acid via isochorismate.

2 l to allow formation of a hydrogen bond with isochorismate.

3 njugate addition of alpha-ketoglutarate with isochorismate.

4 cular Arg106 are critical for recognition of isochorismate.

5 om chorismate and water via the intermediate isochorismate.

6 of the inactive D38A variant in complex with isochorismate.

7 e enzymatic hydroxymutation of chorismate to isochorismate.

8 tic data indicate that for the production of isochorismate, a high magnesium ion concentration suppre

9 initiated by the conversion of chorismate to isochorismate, a reaction that is catalyzed by the menaq

10 previously, that might be important for the isochorismate activity of the EntC.

11 se two residues are important for binding of isochorismate and for stabilizing the cofactor position.

12 he crystal structure of EntC in complex with isochorismate and Mg(2+)at 2.3 A resolution, the first s

13 s to establish the kinetic mechanisms of the isochorismate and salicylate synthase enzymes of siderop

14 The proximity of aspartate 38 to isochorismate and the complete loss of activity resultin

15 residues in the conversion of chorismate to isochorismate and to obtaining clues about the pyruvate

16 The structures reveal that isochorismate binds to the PhzD active site in a trans-d

17 lyzes hydrolysis of the related vinyl ethers isochorismate, chorismate, and 4-amino-4-deoxychorismate

18 orders of magnitude higher affinity for the isochorismate complex relative to the chorismate complex

19 lyzed activities, as well as the uncatalyzed isochorismate decomposition, are reported from temperatu

20 ismate synthase (ICS) converts chorismate to isochorismate for the biosynthesis of phylloquinone, an

21 ically produces salicylate and pyruvate from isochorismate for ultimate incorporation of the salicyla

22 , catalyzing the conversion of chorismate to isochorismate (IC) in a reaction that operates near equi

23 SgcD joins isochorismate (IC) synthase and 4-amino-4-deoxychorismat

24 te to salicylate without the accumulation of isochorismate in solution.

25 the irreversible conversion of chorismate to isochorismate in the presence of Mg2+.

26 In Escherichia coli, isochorismate is a common precursor for the biosynthesis

27 btI at 2.5 A resolution and demonstrate that isochorismate is a kinetically competent intermediate in

28 dicates that the methylene pyruvyl carbon of isochorismate is adjacent to the side chain carboxylate

29 Moreover, we show that isochorismate is channeled through the synthase reaction

30 Isochorismate is formed by the shikimate pathway from ch

31 At pH values below 7.5 isochorismate is the dominant product while above this p

32 encoded by angB and designated AngB, has an isochorismate lyase activity necessary for the synthesis

33 The N-terminal 187 amino acids of EntB (isochorismate lyase domain) are not needed for reaction

34 show that EntB, previously described as the isochorismate lyase required for production of 2,3-DHB,

35 monophosphate ligase, VibB is a bifunctional isochorismate lyase-aryl carrier protein (ArCP), and Vib

36 the enzymes of phylloquinone synthesis from isochorismate may form a complex in the chloroplast stro

37 ntC, MenF and Irp9 all convert chorismate to isochorismate, only Irp9 subsequently exhibits isochoris

38 The isomerase enzymes release isochorismate or aminodeoxychorismate as the product, wh

39 lant Arabidopsis, it was discovered that the isochorismate pathway is the major source of SA during S

40 -ketoglutarate and with the second substrate isochorismate positioned to accept nucleophilic attack h

41 ochorismate, only Irp9 subsequently exhibits isochorismate pyruvate lyase activity resulting in the f

42 o this class is the enzyme PchB, an 11.4-kDa isochorismate pyruvate lyase from Pseudomonas aeruginosa

43 smate mutase activity similar to that of the isochorismate pyruvate lyase, PchB, from Pseudomonas aer

44 PchB is an isochorismate-pyruvate lyase from Pseudomonas aeruginosa

45 The isochorismate-pyruvate lyase from Pseudomonas aeruginosa

46 The isochorismate-pyruvate lyase from Pseudomonas aeruginosa

47 An isochorismate-pyruvate lyase with adventitious chorismat

48 pathogen-induced accumulation of SA requires isochorismate synthase (AtICS1).

49 ase (MenF) is distinct from the entC-encoded isochorismate synthase (EntC) involved in enterobactin b

50 s Pseudomonas aeruginosa synthesize SA using isochorismate synthase (ICS) and pyruvate lyase.

51 the phenylalanine ammonia lyase (PAL) or the isochorismate synthase (ICS) catalyzed steps.

52 Isochorismate synthase (ICS) converts chorismate to isoc

53 induced SA biosynthesis proceeds through the isochorismate synthase (ICS) pathway, with cold inductio

54 AtICS1 acts as a monofunctional isochorismate synthase (ICS), catalyzing the conversion

55 ing that SA synthesized via PAL, and not via isochorismate synthase (ICS), mediates lesion developmen

56 The pathogen-inducible isochorismate synthase (ICS1) promoter was fused to fire

57 ed in chloroplasts from chorismic acid by an isochorismate synthase (ICS1); SA biosynthesis is negati

58 so inhibit two other enzymes in this family, isochorismate synthase (IS) and anthranilate synthase (A

59 hsis and shares a common core mechanism with isochorismate synthase (IS) and anthranilate synthase (A

60 amino-4-deoxychorismate synthase (ADCS), and isochorismate synthase (IS) are homologous enzymes that

61 (AS), aminodeoxychorismate synthase (ADCS), isochorismate synthase (IS), and salicylate synthase (SS

62 This isochorismate synthase (MenF) is distinct from the entC-

63 enhanced disease susceptibility 1 (EDS1) and isochorismate synthase 1 (ICS1) was identified.

64 Silencing of isochorismate synthase 1 (ICS1), required for salicylic

65 tional induction of the key synthetic enzyme isochorismate synthase 1 (ICS1).

66 The salicylate and isochorismate synthase activities of MbtI are Mg2+-depen

67 kimate pathway from chorismate by the enzyme isochorismate synthase encoded by the entC gene.

68 te to isochorismate, which is mediated by an isochorismate synthase encoded by the menF gene.

69 he role of magnesium ions, which inhibit the isochorismate synthase enzymes but not the salicylate sy

70 is of SA and Ybt by DC3000 requires pchA, an isochorismate synthase gene in the Ybt genomic cluster,

71 Bacillus subtilis has duplicate isochorismate synthase genes, menF and dhbC.

72 Isochorismate synthase is involved in the biosynthesis o

73 lts led to the demonstration of an alternate isochorismate synthase specifically involved in menaquin

74 Silencing of ICS (isochorismate synthase), NPR1 (nonexpresser of pathogene

75 35% identical to the Bs menaquinone-specific isochorismate synthase, MenF, illustrating an example of

76 hat is catalyzed by the menaquinone-specific isochorismate synthase, MenF.

77 ther chorismate-utilizing enzymes, including isochorismate synthase, suggests that they too may bind

78 ing for wild-type and SA biosynthetic mutant isochorismate synthase1 (ics1) Arabidopsis from 0 to 7 d

79 mologue AtrbohD and the SA biosynthesis gene ISOCHORISMATE SYNTHASE1 (ICS1).

80 ancestor (the MST family), that includes the isochorismate synthases and anthranilate synthases.

81 EntC, one of two isochorismate synthases in Escherichia coli, is specific

82 njugate addition of alpha-ketoglutarate with isochorismate to release 2-succinyl-5-enolpyruvyl-6-hydr

83 tamin K2) is the conversion of chorismate to isochorismate, which is mediated by an isochorismate syn