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1 mployed as an alternative or supplement with isoelectric adjustment to refine the biophysical charact
7 nt shifts were more commonly associated with isoelectric depolarizations (median 2 min 36 s), whereas
9 N-acetylcysteine is associated with delay of isoelectric EEG, depletion of GRODs, and subjective bene
10 assessed every 6-12 months until they had an isoelectric electroencephalogram (EEG, attesting to a ve
11 poor neurological outcome of low-voltage and isoelectric electroencephalogram patterns 24 hrs after r
12 ve value for poor outcome of low-voltage and isoelectric electroencephalogram patterns was 68% (confi
13 Seizure control without suppression-burst or isoelectric electroencephalogram predicted good function
15 of polymorphic delta, burst suppression, or isoelectric electroencephalography), attempts to use sta
16 Mass-spectrometric proteomic analysis of isoelectric focused (IEF) cerebrospinal fluid (CSF) immu
17 paration techniques, observable by capillary isoelectric focusing (cIEF) and ion exchange chromatogra
18 ion technology in combination with capillary isoelectric focusing (CIEF) and the imaging plug flow (i
23 te feasibility of a combination of capillary isoelectric focusing (CIEF) in a tapered fused silica (F
29 of each fraction were separated by capillary isoelectric focusing (cIEF) using a set of amino acids a
30 with tapered geometry suitable for capillary isoelectric focusing (CIEF) with electroosmotic displace
31 pillary hybrid device to integrate capillary isoelectric focusing (CIEF) with parallel capillary sodi
32 (boronate-affinity chromatography, capillary isoelectric focusing (CIEF), and standardized DCA2000+ i
33 ion mass spectrometry (ESI-MS) and capillary isoelectric focusing (cIEF), which showed that 0-8 DM1s
37 A combination of time-resolved microfluidic isoelectric focusing (IEF) and in situ antibody blotting
38 analyzed the proteome of T. pallidum by the isoelectric focusing (IEF) and nonequilibrating pH gel e
39 WB) milk/dairy products based on concomitant isoelectric focusing (IEF) detection of B gamma2- and ga
42 ase related amyloid beta (Abeta) peptides by isoelectric focusing (IEF) in 75 nL microchannels combin
44 s paradoxical result, we applied a capillary isoelectric focusing (IEF) method to determine the patte
46 ingle strand conformation polymorphism), and isoelectric focusing (IEF) of water-soluble proteins of
47 surface and deep seawater by two-dimensional isoelectric focusing (IEF) OFFGEL-lab-on-chip (LOC) elec
48 lected glycoproteomes were then subjected to isoelectric focusing (IEF) separation by a digital Prote
49 l (2D) chromatography platform that includes isoelectric focusing (IEF) through immobilized pH gradie
52 cs, use of peptide markers, electrophoresis, isoelectric focusing (IEF)-double immunoblotting, aptame
57 pproaches [native, urea, acid urea PAGEs and isoelectric focusing (IEF)] as the first-dimension (1-DE
58 inator device to our multijunction capillary isoelectric focusing (MJ-CIEF) instrument and perform di
59 ulting in glass-based microfluidic free-flow isoelectric focusing (muFFIEF) chips with integrated pH
60 introduced an online multijunction capillary isoelectric focusing (OMJ-CIEF) fractionator to fraction
61 lian cells, we report single-cell resolution isoelectric focusing (scIEF) and high-selectivity immuno
65 ol against prefractionation of peptides with isoelectric focusing and demonstrate that FAIMS fraction
68 different backgrounds using a combination of isoelectric focusing and liquid chromatography coupled w
72 d demonstrate its feasibility for performing isoelectric focusing and simultaneous fractionation of p
73 Embryonic Kidney 293 cells expressing TRPV5, isoelectric focusing and total internal reflection fluor
76 a new technique that is related to capillary isoelectric focusing but uses additional high-voltage po
78 eight complexes and Blue Native/SDS-PAGE and isoelectric focusing demonstrated that different isoform
79 iquots which were analyzed in parallel by 1D isoelectric focusing electrophoresis (IEF)-laser ablatio
80 d by charge-based analytical methods such as isoelectric focusing electrophoresis and cation or anion
83 c proteomic immunoassay (NIA), consisting of isoelectric focusing followed by sensitive chemiluminesc
86 roduce an online multiple-junction capillary isoelectric focusing fractionator (OMJ-CIEF) for separat
88 s therefore a good choice to couple directly isoelectric focusing gel electrophoresis with mass spect
89 high peak capacity demonstrates that dynamic isoelectric focusing has great potential as a first dime
93 noprobed isoelectric focusing is composed of isoelectric focusing in a large pore-size polyacrylamide
95 ecting protein phosphorylation that involves isoelectric focusing in a vertical mini-gel format follo
102 n emerging separation method for preparative isoelectric focusing of complex peptide mixtures, as a t
103 imensional peptide separation methods (here, isoelectric focusing of peptides) are incorporated into
107 cence difference labeling followed by liquid isoelectric focusing prefractionation/two-dimensional ge
110 absolute quantification approach with OFFGEL isoelectric focusing separation and liquid chromatograph
111 system, we could visualize a high resolution isoelectric focusing signature of PKCdelta upon stimulat
112 modifications of BSA were investigated by 2D isoelectric focusing sodium dodecyl sulfate polyacrylami
114 diac myofibrils separated by two-dimensional isoelectric focusing subjected to a Western blot with a
115 egrated and rapid multispectral immunoprobed isoelectric focusing technique for identifying specific
116 utation prompted us to develop an analytical isoelectric focusing technique that resolves endogenous
117 a rapid and efficient procedure utilizing an isoelectric focusing technique to obtain vaccines from t
120 uestion, we used affinity chromatography and isoelectric focusing to fractionate plasma immunoglobuli
121 ese DNA-assisted RCA techniques in capillary isoelectric focusing to resolve endogenous signaling tra
126 chromatography, native gel electrophoresis, isoelectric focusing, and analytical sedimentation equil
127 eomics, HPLC with electrochemical detection, isoelectric focusing, and extraction of acidic and basic
128 chromatography, native gel electrophoresis, isoelectric focusing, and sedimentation equilibrium.
129 he investigations involved phenotypic tests, isoelectric focusing, beta-lactamase inhibitor studies,
130 cyanine CyDye DIGE Fluor minimal dyes before isoelectric focusing, enabling the labeling of 2-3 sampl
132 , and with a spatial resolution matching the isoelectric focusing, it is therefore a good choice to c
133 ophagus cell line and separated by capillary isoelectric focusing, reproducibly generating dozens of
134 s documented by mass spectrometry, SDS-PAGE, isoelectric focusing, size-exclusion chromatography with
135 mass without resorting to ampholytes and/or isoelectric focusing, using a single- and two-step free-
137 sisting of a single 3.75 cm long channel for isoelectric focusing, which is sampled in parallel by 20
142 lysis detected a HLA-A H chain with a unique isoelectric-focusing pattern, which does not associate w
145 The NanoPro is a multiplexed capillary-based isoelectric immunoassay with whole-column imaging detect
148 after cardiac arrest, the combined group of isoelectric, low voltage, and "burst-suppression with id
151 seed flour, followed by precipitation at the isoelectric pH, yielded a 92% protein isolate with a hig
152 asic protein (MBP1) is an exceedingly basic (isoelectric point >11) 14-kDa protein, comprising the co
155 re unstable to flocculation near the protein isoelectric point (pH 5.0), at high ionic strength (>100
158 proteins was affected by differences in the isoelectric point (pI) and the ion exchange properties o
160 f integrated microfluidic platforms for fast isoelectric point (pI) determinations via free-flow elec
161 otoheterotrophic life style and a low median isoelectric point (pI) for all predicted proteins, sugge
163 ble minor structural protein (ORF3), with an isoelectric point (pI) of 10.0 and a calculated molecula
165 f the buffer is merely one pH unit below the isoelectric point (pI) of the recombinant proteins, most
166 contribution was ruled out by comparing the isoelectric point (pI) values to the adsorption of prote
167 ome key parameters, such as applied voltage, isoelectric point (pI), bulk pH, and bulk conductivity,
172 ry peak of expression of genes encoding high-isoelectric point alpha-amylase during grain development
176 easing pH and strong agglomeration above the isoelectric point and settling in the presence of Ca.
178 emonstrated with amidation to modify protein isoelectric point and through derivatization with quater
179 explains the surprising observation that the isoelectric point appeared at approximately 3.0 when HCl
180 emistry and proteomics methods depend on the isoelectric point as a principal feature for protein and
182 se of solution pH from 5.0 to 6.0 toward the isoelectric point as well as more rapid dissociation of
183 d is likely facilitated by its difference in isoelectric point compared to the other VP sequences ass
185 y of the sample protein is reduced above the isoelectric point due to the decreased electrostatic att
187 aphid proteins were expressed as heritable, isoelectric point isoform pairs, one derived from each p
190 -A, with a molecular mass of 69.4 kDa and an isoelectric point of 3.5 was purified from papaya latex.
193 ed 12 transmembrane helices and predicted an isoelectric point of 9.4, both of which are characterist
194 mL) was treated with ammonium sulfate at the isoelectric point of alpha-Cbtx, and the pellet was diss
195 ein expression but did induce changes in the isoelectric point of Bim(EL) and its reactivity with an
196 H 7 as bulk water), a value that matches the isoelectric point of bubbles and oil droplets in indepen
200 s, TPT could change the molecular weight and isoelectric point of effector proteins, induce their met
201 significant shift toward lower values of the isoelectric point of maghemite upon selenium(IV) uptake,
204 of the GPI-anchor to the molecular mass and isoelectric point of PrP quasispecies under two-dimensio
205 evices also allowed for determination of the isoelectric point of the minute amounts of proteins immo
206 on procedure based on the molecular size and isoelectric point of the photoradiolabeled binding prote
207 ble to aggregation at pH values close to the isoelectric point of the protein (pH 4 and 5), at high i
208 e in charge density on the bead surface, the isoelectric point of the protein then dominates the mobi
211 lution isoelectric focusing to determine the isoelectric point of Wnt3A protein in conditioned medium
213 eptides of both hydrolysates showed that low isoelectric point peptides had antioxidant activity; how
216 ed for polymers, by linking photolysis to an isoelectric point shift, which itself is linked to a sol
217 del peptides differing in hydrophobicity and isoelectric point using a combination of ion-exchange an
218 resumably normosialylated based on a neutral isoelectric point) in other glomerular diseases as well.
220 hat contains the predicted molecular weight, isoelectric point, accession number, and putative functi
222 Moreover, NTHi P5 has a high theoretical isoelectric point, calculated as 9.58, which is not in a
223 ne adjustment of the buffer pH, close to the isoelectric point, can be used to slow down the transloc
224 ch is not in agreement with the experimental isoelectric point, determined as 6.3-6.8, or with its pr
225 gical properties of MTases: structural fold, isoelectric point, expression pattern and cellular local
226 parameters, such as crosslinking density and isoelectric point, have been tuned in order to optimize
227 fold more "extreme" proteins (low mass, high isoelectric point, hydrophobic) than previous mitochondr
228 ial dilatational rheology was highest at the isoelectric point, indicating the dominant role of the c
229 o acids in different physicochemical groups, isoelectric point, molecular weight, and length of prote
230 re stable, reproducibly displayed a shift in isoelectric point, or changed in relative abundance at s
231 surface varies, and if it is lower than the isoelectric point, that factor increases (rectification
235 c system-for example protein, DNA or RNA-the isoelectric point-commonly referred to as the pI-can be
239 aganeite can be explained both by the higher isoelectric point/point-of-zero charge (9.6-10) of this
240 on electrostatic attraction between the low-isoelectric-point enzyme and the microstructure, fabrica
242 ds; P22 Xis, 116 amino acids) and have basic isoelectric points (for P22 Xis, 9.42; for lambda Xis, 1
243 hree fractions: one containing proteins with isoelectric points (pI values) higher than the pI of alb
244 lted in a cluster of ill-resolved peaks with isoelectric points (pI values) in the range 7.4 to 8.5.
245 n capabilities, using compounds with varying isoelectric points (pI) and pK(a) values over a range of
246 population was observed to exhibit distinct isoelectric points (pI) and, thus, distinct formal elect
247 mn imaging detection (CIEF-WCID) method, the isoelectric points (pI) of complete intact polioviruses
248 ly discretized into fractions based on their isoelectric points (pI) without the use of carrier ampho
249 ion of acidic peptides (i.e., those with low isoelectric points (pI)) by matrix-assisted laser desorp
250 rogeneous organelles that likely have unique isoelectric points (pI), which are related to their surf
251 roteins in surface seawater exhibit alkaline isoelectric points (pIs of 8.10 and 8.37) and also acid
252 de mixtures based on the difference in their isoelectric points (pIs) after methyl esterification.
253 ncated 25- to 27-kDa isoforms having various isoelectric points and electrophoretic mobilities were e
254 utralization breadth have relatively neutral isoelectric points and preferentially bind to envelope m
255 are consistently distinguished by more basic isoelectric points and specificity for epitopes shared b
256 Subsequently, we added estimates of protein isoelectric points as a feature to ANNs that classify sp
257 , we found inducible oligomers and shifts in isoelectric points for peroxiredoxin 1 (Prdx-1), Prdx-3,
258 t of native Hs11S was about 150-300 kDa with isoelectric points of 5.0-5.3, composed by four monomers
259 molecular weight of around 7.3 kDa, a basic isoelectric points of 8.5 and the presence of typical CX
260 ifications contribute to the measured acidic isoelectric points of Cx26 and Cx32, whereas their low m
261 ints of the homomeric channels bracketed the isoelectric points of heteromeric Cx26/Cx32 channels.
262 the first report of the determination of the isoelectric points of individual mitochondria by capilla
264 In this study, size, zeta-potential, and the isoelectric points of nanoparticles of the recombinant f
265 rus transmission-competent F2 genotypes, the isoelectric points of the Buchnera proteins did not matc
268 at are in good agreement with the respective isoelectric points of Tim9 and Tim10 were determined.
271 e of molecular mass, hydrodynamic radii, and isoelectric points through a nanofiber hydrogel scaffold
275 roteins with different molecular weights and isoelectric points when a low-femtomole amount was loade
276 assay was applied to several mAbs of varying isoelectric points, demonstrating the suitability of Nan
277 o proteins with low molecular weight and low isoelectric points, distinguished fresh fillets from fro
278 somes have unique features (e.g., very basic isoelectric points, expressing the mutated tumor antigen
279 y extracting peptides, on the basis of their isoelectric points, into an immiscible organic phase fro
280 rley and wheat were found to differ in their isoelectric points, resistance to proteolysis, and conta
284 ted on the basis of close proximity of their isoelectric points: D. chrysanthemi, D. chrysanthemi bv.
286 er in the presence of sucrose or by applying isoelectric precipitation at pH 5.0 and then diluting th
287 fast extraction method allowed to obtain an isoelectric precipitation protein concentrate (IPPC) wit
288 late extracted from Arthrospira platensis by isoelectric precipitation was evaluated for its function
295 k place in spontaneously active cortex or as isoelectric spreading depolarisation (ISD) if they took
297 ain activity levels from light anesthesia to isoelectric state, to examine whether the in vivo (31)P
299 ists of a quasi-periodic alternation between isoelectric 'suppressions' lasting seconds or minutes, a
300 ate that the behavior of Mb, which is nearly isoelectric under the conditions used, is well described
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