ライフサイエンスコーパス: isoelectric focusing

1 yacrylamide could be excellent for capillary isoelectric focusing.

2 uous separation of subcellular organelles by isoelectric focusing.

3 of them are successful for routine capillary isoelectric focusing.

4 M cone opsins by in situ phosphorylation and isoelectric focusing.

5 monoclonal antibody HC-10, and 2-dimensional isoelectric focusing.

6 ed biochemically by CDK2 kinase assay and by isoelectric focusing.

7 dic shift of r-p53 isoforms separated by gel isoelectric focusing.

8 rms of the enzymes were identified by native isoelectric focusing.

9 nd that 16 (1.2%) demonstrated AmpC bands by isoelectric focusing.

10 at least two different isoforms separable by isoelectric focusing.

11 n intermolecular exchange as shown by native isoelectric focusing.

12 n were then purified by SDS-PAGE followed by isoelectric focusing.

13 from the dye-stained blots and subjected to isoelectric focusing.

14 uring polyacrylamide gel electrophoresis and isoelectric focusing.

15 uring polyacrylamide gel electrophoresis and isoelectric focusing.

16 isolates, 83 (9.2%) produced ESBLs based on isoelectric focusing.

17 ession of class I heavy chains as defined by isoelectric focusing.

18 ting after separation by polyacrylamide-urea isoelectric focusing.

19 filtration, or affinity chromatography or by isoelectric focusing.

20 e concentration required for the traditional isoelectric focusing.

21 issues have discouraged its use in capillary isoelectric focusing.

22 High-resolution capillary isoelectric focusing allows isoforms and individual phos

23 sulfate-polyacrylamide gel electrophoresis, isoelectric focusing, amino acid analysis, and mass spec

24 Isoelectric focusing analyses showed that sera contained

25 Isoelectric focusing analysis of eIF2alpha followed by d

26 Isoelectric focusing analysis revealed that the product

27 Moreover, using isoelectric focusing analysis, we show that both pro-FD

28 Isoelectric-focusing analysis detected a HLA-A H chain w

29 semia (D/D) have a characteristic isoform on isoelectric focusing and 50% reduction in galactose-1-ph

30 We used dynamic capillary isoelectric focusing and boronate affinity chromatograph

31 ol against prefractionation of peptides with isoelectric focusing and demonstrate that FAIMS fraction

32 grity of the more thermolabile mTORC2 during isoelectric focusing and detection (<45 min).

33 ctamases were identified by a combination of isoelectric focusing and gene sequencing.

34 We used isoelectric focusing and immunoblotting experiments to d

35 IgM bands were studied by isoelectric focusing and immunodetection.

36 an efficient procedure involving preparative isoelectric focusing and ion exchange FPLC chromatograph

37 different backgrounds using a combination of isoelectric focusing and liquid chromatography coupled w

38 We combined peptide isoelectric focusing and liquid chromatography-tandem ma

39 Isoelectric focusing and mass spectrometry demonstrate t

40 and identified using the above method where isoelectric focusing and restriction enzyme analysis fai

41 using gel permeation chromatography, OFFGEL isoelectric focusing and RP-HPLC.

42 is paper we have applied sequential solution isoelectric focusing and SDS-PAGE for the proteomic char

43 Using high resolution isoelectric focusing and SDS-polyacrylamide gel electrop

44 d demonstrate its feasibility for performing isoelectric focusing and simultaneous fractionation of p

45 mplexes were purified using a combination of isoelectric focusing and sucrose gradient ultracentrifug

46 Embryonic Kidney 293 cells expressing TRPV5, isoelectric focusing and total internal reflection fluor

47 referred to as membrane separated wells for isoelectric focusing and trapping (MSWIFT).

48 ation was demonstrated using high-resolution isoelectric focusing and two-dimensional gel analysis.

49 chromatography, native gel electrophoresis, isoelectric focusing, and analytical sedimentation equil

50 nked immunosorbent assay for IgG and IgM, by isoelectric focusing, and by inhibition studies to deter

51 eomics, HPLC with electrochemical detection, isoelectric focusing, and extraction of acidic and basic

52 ei CYA was then partially purified by liquid isoelectric focusing, and fractions were subjected to so

53 and characterized by Western blot analysis, isoelectric focusing, and kinetic studies.

54 fferential centrifugation and by preparative isoelectric focusing, and purified to homogeneity on two

55 e extraction, anion exchange chromatography, isoelectric focusing, and reverse-phase high performance

56 chromatography, native gel electrophoresis, isoelectric focusing, and sedimentation equilibrium.

57 The results of 2-dimensional isoelectric focusing are consistent in showing P-selecti

58 eight) isoforms of Mcm1 protein, resolved by isoelectric focusing, are present in vivo; two major pho

59 This study establishes free-flow isoelectric focusing as a valuable tool for characterizi

60 The two dimensions involve the use of liquid isoelectric focusing as the first phase and nonporous si

61 -linked oligosaccharides from the individual isoelectric focusing bands of a glycoprotein using recom

62 ies could be resolved into discrete forms by isoelectric focusing based apparently on their arabinan

63 Through the use of capillary isoelectric focusing-based multidimensional peptide sepa

64 in the plasma was estimated by quantitative isoelectric focusing before and after liver transplantat

65 he investigations involved phenotypic tests, isoelectric focusing, beta-lactamase inhibitor studies,

66 and concentrated in a manner that resembles isoelectric focusing but that is applicable to a greater

67 a new technique that is related to capillary isoelectric focusing but uses additional high-voltage po

68 s mixture was fractionated using preparative isoelectric focusing by free-flow electrophoresis, follo

69 Dynamic isoelectric focusing can achieve a peak capacity of over

70 tography, and the isozymes were separated by isoelectric focusing, characterized, and quantitated.

71 nzyme by anion-exchange, gel filtration, and isoelectric focusing chromatography yielded a protein mo

72 ion of the powerful combination of capillary isoelectric focusing (CIEF) and Fourier transform ion cy

73 paration techniques, observable by capillary isoelectric focusing (cIEF) and ion exchange chromatogra

74 ion technology in combination with capillary isoelectric focusing (CIEF) and the imaging plug flow (i

75 By comparing with capillary isoelectric focusing (CIEF) as another electrokinetics-b

76 Nanocapillary isoelectric focusing (cIEF) combines with UV-activated l

77 Capillary isoelectric focusing (CIEF) coupled with electrospray io

78 Capillary isoelectric focusing (CIEF) coupled with reversed-phase

79 lex protein mixtures, separated by capillary isoelectric focusing (CIEF) for detection using on-line

80 d-beta (Abeta) peptide variants by capillary isoelectric focusing (CIEF) immunoassay.

81 te feasibility of a combination of capillary isoelectric focusing (CIEF) in a tapered fused silica (F

82 Capillary isoelectric focusing (cIEF) is normally run under denatu

83 into a single capillary tube where capillary isoelectric focusing (CIEF) is performed.

84 Capillary isoelectric focusing (cIEF) is widely used in the biopha

85 A capillary isoelectric focusing (cIEF) method has been developed as

86 e liquid chromatography (CIEX) and capillary isoelectric focusing (cIEF) methods have been developed

87 In the present work, capillary isoelectric focusing (CIEF) methods were developed for t

88 ivity advantages over conventional capillary isoelectric focusing (CIEF) platforms.

89 k, methods for the high-efficiency capillary isoelectric focusing (CIEF) separation of peptides have

90 a first step toward adaptation of capillary isoelectric focusing (cIEF) to microchannels on a glass

91 of each fraction were separated by capillary isoelectric focusing (cIEF) using a set of amino acids a

92 h involving on-line combination of capillary isoelectric focusing (CIEF) with capillary reversed-phas

93 with tapered geometry suitable for capillary isoelectric focusing (CIEF) with electroosmotic displace

94 On-line combination of capillary isoelectric focusing (CIEF) with electrospray ionization

95 strated for on-line combination of capillary isoelectric focusing (CIEF) with electrospray ionization

96 pillary hybrid device to integrate capillary isoelectric focusing (CIEF) with parallel capillary sodi

97 terface for on-line combination of capillary isoelectric focusing (CIEF) with transient capillary iso

98 (boronate-affinity chromatography, capillary isoelectric focusing (CIEF), and standardized DCA2000+ i

99 ion mass spectrometry (ESI-MS) and capillary isoelectric focusing (cIEF), which showed that 0-8 DM1s

100 On-line capillary isoelectric focusing (CIEF)-electrospray ionization mass

101 ints of individual mitochondria by capillary isoelectric focusing (cIEF).

102 Capillary isoelectric focusing combined with nano-reversed-phase l

103 Metabolic labeling or separation by isoelectric focusing confirmed the presence of phosphory

104 f compounds is presented, based on capillary isoelectric focusing coupled on-line with electrospray i

105 Western blot analysis and isoelectric focusing demonstrate that, although migratin

106 lyacrylamide-gel electrophoresis and also by isoelectric focusing demonstrate the presence of an acid

107 eight complexes and Blue Native/SDS-PAGE and isoelectric focusing demonstrated that different isoform

108 When analyzed after folding by isoelectric focusing, each chain yielded only one band,

109 iquots which were analyzed in parallel by 1D isoelectric focusing electrophoresis (IEF)-laser ablatio

110 The protein was purified to homogeneity by isoelectric focusing electrophoresis (pI 5.8), and it ap

111 d by charge-based analytical methods such as isoelectric focusing electrophoresis and cation or anion

112 Isoelectric focusing electrophoresis and mass spectromet

113 were purified and subsequently separated by isoelectric focusing electrophoresis.

114 On-line capillary isoelectric focusing-electrospray ionization mass spectr

115 cyanine CyDye DIGE Fluor minimal dyes before isoelectric focusing, enabling the labeling of 2-3 sampl

116 IDS is analogous to isoelectric focusing, except instead of separating ampho

117 nic matter fraction ("water extract") via an isoelectric focusing experiment (IEF).

118 Isoelectric focusing experiments demonstrate that the DU

119 Subzero isoelectric focusing experiments suggest that rHb Oscar

120 Isoelectric focusing experiments were carried out on all

121 caded stages for a microfabricated free-flow isoelectric focusing (FF-IEF) device.

122 nslational modifications and a change in the isoelectric focusing fingerprints relative to the parent

123 Apo E phenotyping was done with isoelectric focusing followed by immunostaining; Lp(a) w

124 whole saliva, and pellicle were subjected to isoelectric focusing followed by SDS-PAGE.

125 c proteomic immunoassay (NIA), consisting of isoelectric focusing followed by sensitive chemiluminesc

126 Isoelectric focusing followed by Western blot analysis o

127 Moreover, CSF isoelectric focusing followed by western blot assays rev

128 gradient centrifugation as an alternative to isoelectric focusing for protein separation in the first

129 We report the application of capillary isoelectric focusing for quantitative analysis of a comp

130 I, and separated from the native species by isoelectric focusing for use in solid phase binding assa

131 roduce an online multiple-junction capillary isoelectric focusing fractionator (OMJ-CIEF) for separat

132 Subunits were purified by isoelectric focusing from extracts of cells that overpro

133 By using both nondenaturing isoelectric focusing gel electrophoresis and fast-perfor

134 ified using immunoprecipitation, followed by isoelectric focusing gel electrophoresis and specific TR

135 Genome sequencing and isoelectric focusing gel electrophoresis showed that the

136 s therefore a good choice to couple directly isoelectric focusing gel electrophoresis with mass spect

137 In addition, as judged by isoelectric focusing gel electrophoresis, the relative l

138 ve protein on both native polyacrylamide and isoelectric focusing gel electrophoresis.

139 Mass spectrometric surface analysis of isoelectric focusing gels provides an ultrasensitive app

140 Activity staining of isoelectric focusing gels showed that cytosolic ALDH1 co

141 sis of Triton x-100 insoluble fractions, 2-D isoelectric focusing gels, and confocal microscopy.

142 When eluted from isoelectric focusing gels, the acidic matrix glycoprotei

143 ndividual bands following electroblotting of isoelectric focusing gels.

144 to which the nuclear Elf-1 form separated in isoelectric focusing gels.

145 high peak capacity demonstrates that dynamic isoelectric focusing has great potential as a first dime

146 crease of basic variants in imaged capillary isoelectric focusing (iCIEF) analysis.

147 evice that sequentially couples liquid-phase isoelectric focusing (IEF) and free solution capillary e

148 A combination of time-resolved microfluidic isoelectric focusing (IEF) and in situ antibody blotting

149 analyzed the proteome of T. pallidum by the isoelectric focusing (IEF) and nonequilibrating pH gel e

150 WB) milk/dairy products based on concomitant isoelectric focusing (IEF) detection of B gamma2- and ga

151 fied through a series of ultrafiltration and isoelectric focusing (IEF) electrophoresis steps.

152 e CSL was isolated from whole sporozoites by isoelectric focusing (IEF) following observations that t

153 eparate the precolumn labeled proteins using isoelectric focusing (IEF) have been made.

154 ase related amyloid beta (Abeta) peptides by isoelectric focusing (IEF) in 75 nL microchannels combin

155 rotocol that improves the resolving power of isoelectric focusing (IEF) in a polymeric microfluidic c

156 rt of an ongoing investigation of the use of isoelectric focusing (IEF) in microfluidic devices, pH g

157 e of gold or palladium to perform transverse isoelectric focusing (IEF) is presented as a means for c

158 Isoelectric focusing (IEF) is the first step for two-dim

159 s paradoxical result, we applied a capillary isoelectric focusing (IEF) method to determine the patte

160 After isoelectric focusing (IEF) of a methylated tryptic diges

161 Isoelectric focusing (IEF) of Cx43 in HuH-7 cells indica

162 ingle strand conformation polymorphism), and isoelectric focusing (IEF) of water-soluble proteins of

163 surface and deep seawater by two-dimensional isoelectric focusing (IEF) OFFGEL-lab-on-chip (LOC) elec

164 After separation by isoelectric focusing (IEF) or non-equilibrium pH gradien

165 t forms of CDGs can be recognized by altered isoelectric focusing (IEF) patterns of serum transferrin

166 lected glycoproteomes were then subjected to isoelectric focusing (IEF) separation by a digital Prote

167 Analysis of C/EBPbeta phosphorylation by isoelectric focusing (IEF) shows that LAP is present in

168 nes bla(TEM), bla(SHV), and bla(OXA), and in isoelectric focusing (IEF) tests, all isolates demonstra

169 l (2D) chromatography platform that includes isoelectric focusing (IEF) through immobilized pH gradie

170 Sub-zero isoelectric focusing (IEF) was employed to investigate t

171 The new methods couple isoelectric focusing (IEF) with high ionic strength elec

172 tion/separation system, combining non-native isoelectric focusing (IEF) with sodium dodecyl sulfate (

173 By combining isoelectric focusing (IEF) with subsequent gel electroph

174 By isoelectric focusing (IEF), 7 of the 23 isolates contain

175 Compared to isoelectric focusing (IEF), the CZE method provides more

176 cs, use of peptide markers, electrophoresis, isoelectric focusing (IEF)-double immunoblotting, aptame

177 per-based test were compared to conventional isoelectric focusing (IEF).

178 oteins is their natural incompatibility with isoelectric focusing (IEF).

179 etermined, and heterogeneity was analyzed by isoelectric focusing (IEF).

180 ration and cup-loading methods were used for isoelectric focusing (IEF).

181 Protein analyses by two-dimensional isoelectric focusing (IEF)/SDS-PAGE not only revealed di

182 pproaches [native, urea, acid urea PAGEs and isoelectric focusing (IEF)] as the first-dimension (1-DE

183 We conclude that the capillary isoelectric focusing immunoassay system may provide a wi

184 Using a capillary isoelectric focusing immunoassay system, we could visual

185 Protein complexes were separated using isoelectric focusing in a capillary under native conditi

186 The samples can be first separated by isoelectric focusing in a gel and then quickly in situ d

187 noprobed isoelectric focusing is composed of isoelectric focusing in a large pore-size polyacrylamide

188 Separation of proteins is achieved by isoelectric focusing in a short length of fused-silica c

189 ecting protein phosphorylation that involves isoelectric focusing in a vertical mini-gel format follo

190 26) and connexin32 (Cx32) were determined by isoelectric focusing in free fluids.

191 soelectric points compared with conventional isoelectric focusing in gels.

192 r reports the application of ampholyte-based isoelectric focusing in poly(dimethylsiloxane) (PDMS) us

193 The proteins are separated by pI using isoelectric focusing in the first dimension and by hydro

194 dimensional gel electrophoresis studies with isoelectric focusing in the first dimension identified n

195 When high-resolution isoelectric focusing, in-gel CNBr cleavage, and mass spe

196 However, analysis by isoelectric focusing indicates two major bands (pI 4.7 a

197 e high resolution of immobilized pH gradient isoelectric focusing (IPG-IEF) for the analysis of these

198 Dynamic isoelectric focusing is a new technique that is related

199 We show that native capillary isoelectric focusing is a powerful method for the diffic

200 Immunoprobed isoelectric focusing is composed of isoelectric focusing

201 , and with a spatial resolution matching the isoelectric focusing, it is therefore a good choice to c

202 ry positioning also allows on-line capillary isoelectric focusing mass spectrometry with in-probe foc

203 We utilized a capillary-based isoelectric focusing method to study dynamics of Akt pho

204 inator device to our multijunction capillary isoelectric focusing (MJ-CIEF) instrument and perform di

205 Since in an isoelectric focusing mode the CA species are present in

206 ulting in glass-based microfluidic free-flow isoelectric focusing (muFFIEF) chips with integrated pH

207 ll with experimental results of microfluidic isoelectric focusing of a single protein species.

208 Isoelectric focusing of cerebrospinal fluid reveals band

209 n emerging separation method for preparative isoelectric focusing of complex peptide mixtures, as a t

210 As an application, isoelectric focusing of green fluorescence protein on th

211 Isoelectric focusing of immunoprecipitated MT from trans

212 Simulations of isoelectric focusing of mitochondria in microchannels ar

213 rate the use of VICAT reagents together with isoelectric focusing of peptides on an immobilized gel s

214 imensional peptide separation methods (here, isoelectric focusing of peptides) are incorporated into

215 all amounts of nucleic acids; more recently, isoelectric focusing of proteins also has been adapted t

216 Isoelectric focusing of proteins in a silica nanofluidic

217 age both DNA electrophoresis separations and isoelectric focusing of proteins.

218 Isoelectric focusing of sample biological analytes, bovi

219 Rapid (<20 min) isoelectric focusing of several fluorescent pI markers a

220 f U87-MG cells or fractions from preparative isoelectric focusing of the lysates.

221 Isoelectric focusing of the proteins in a nondenaturing

222 introduced an online multijunction capillary isoelectric focusing (OMJ-CIEF) fractionator to fraction

223 The thin-layer polyacrylamide gel isoelectric focusing pattern of factor IX purified from

224 e as determined by PCR (with a corresponding isoelectric focusing pattern).

225 lysis detected a HLA-A H chain with a unique isoelectric-focusing pattern, which does not associate w

226 Isoelectric focusing performed with photobleached pH 3-1

227 proteins and by sodium dodecyl sulfate- and isoelectric focusing polyacrylamide gel electrophoresis,

228 MLC-Pi, measured using isoelectric focusing-polyacrylamide gel electrophoresis

229 d anion exchange chromatography, preparative isoelectric focusing, polymer-based C18-like, and analyt

230 cence difference labeling followed by liquid isoelectric focusing prefractionation/two-dimensional ge

231 Glycoproteins often display a complex isoelectric focusing profile because of the presence of

232 ese charged carbohydrates in determining the isoelectric focusing profile has been limited to observi

233 rophoresis: (i) nonreducing/reducing or (ii) isoelectric focusing/reducing SDS/PAGE.

234 ophagus cell line and separated by capillary isoelectric focusing, reproducibly generating dozens of

235 arated by agarose gel electrophoresis and by isoelectric focusing, respectively.

236 Isoelectric focusing results suggested that a predominan

237 Analysis by SDS-PAGE and isoelectric focusing revealed no structural difference b

238 imately 17.5 and 18.5 kDa on SDS-PAGE, while isoelectric focusing revealed seven to eight proteins wi

239 Isoelectric focusing revealed that all of the phosphates

240 lian cells, we report single-cell resolution isoelectric focusing (scIEF) and high-selectivity immuno

241 itro, the modified proteins were analyzed by isoelectric focusing, SDS polyacrylamide electrophoresis

242 analysis, immunoprecipitation, 2-dimensional isoelectric focusing/SDS PAGE and immunoblot analysis sh

243 Analysis by two-dimensional isoelectric focusing/SDS-PAGE suggests that several diff

244 Two-dimensional isoelectric focusing/SDS-polyacrylamide gels were used t

245 Dynamic capillary isoelectric focusing separated hemoglobin A1c into two s

246 Isoelectric focusing separated the 55 kDa band into mult

247 The high efficiency capillary isoelectric focusing separation allowed for resolution o

248 absolute quantification approach with OFFGEL isoelectric focusing separation and liquid chromatograph

249 High-resolution capillary isoelectric focusing separations of complex protein mixt

250 Analysis of the purified 3C protein by isoelectric focusing showed differently charged 3C isofo

251 henotype of adult HPFH transgenic animals by isoelectric focusing showed the presence of hybrid mouse

252 system, we could visualize a high resolution isoelectric focusing signature of PKCdelta upon stimulat

253 s documented by mass spectrometry, SDS-PAGE, isoelectric focusing, size-exclusion chromatography with

254 modifications of BSA were investigated by 2D isoelectric focusing sodium dodecyl sulfate polyacrylami

255 , as judged by separation on two-dimensional isoelectric focusing/sodium dodecyl sulfate-polyacrylami

256 Isoelectric focusing studies revealed that LAMP2 has a m

257 An isoelectric focusing study suggests that some IgG antibo

258 diac myofibrils separated by two-dimensional isoelectric focusing subjected to a Western blot with a

259 We have utilized a free-solution isoelectric focusing technique (FS-IEF) to obtain chaper

260 egrated and rapid multispectral immunoprobed isoelectric focusing technique for identifying specific

261 A new isoelectric focusing technique has been developed that i

262 utation prompted us to develop an analytical isoelectric focusing technique that resolves endogenous

263 a rapid and efficient procedure utilizing an isoelectric focusing technique to obtain vaccines from t

264 By using isoelectric focusing techniques to distinguish RsbV from

265 A previously undescribed isoelectric focusing technology allows cell signaling to

266 mass spectrometry, the expected mobility on isoelectric focusing, the calculated amino acid composit

267 We then used in-solution isoelectric focusing to determine the isoelectric point

268 uestion, we used affinity chromatography and isoelectric focusing to fractionate plasma immunoglobuli

269 ese DNA-assisted RCA techniques in capillary isoelectric focusing to resolve endogenous signaling tra

270 Another approach uses capillary isoelectric focusing to separate bacteria by their surfa

271 zyme forms were separated from each other by isoelectric focusing to show that heterotetramers were f

272 , and can be used, together with transferrin isoelectric focusing, to diagnose patients with CDG-Ia a

273 Capillary isoelectric focusing together with antibody-based detect

274 Here we report the use of capillary isoelectric focusing under native conditions for the sep

275 amide gel electrophoresis (PAGE) and agarose isoelectric focusing under nondenaturing conditions.

276 mass without resorting to ampholytes and/or isoelectric focusing, using a single- and two-step free-

277 In particular, nondenaturing gel isoelectric focusing was combined with alkaline 40% poly

278 Preparative isoelectric focusing was conducted to purify basic prote

279 Haemoglobin electrophoresis by isoelectric focusing was done on all samples to identify

280 Isoelectric focusing was used to evaluate the phosphoryl

281 Using isoelectric focusing, we detected SPAG16-specific oligoc

282 spectrometry (and, for comparative purposes, isoelectric focusing) were used to characterize venoms f

283 In contrast to conventional isoelectric focusing where the fluid does not move, this

284 sisting of a single 3.75 cm long channel for isoelectric focusing, which is sampled in parallel by 20

285 Using a capillary isoelectric focusing-whole column imaging detection (CIE

286 se blood were determined after separation by isoelectric focusing with an octyl-bonded capillary.

287 ormed on a special holder designed to couple isoelectric focusing with SDS-PAGE.