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1 key properties (aromaticity, hydropathy and isoelectric point).
2 ibuted over these sites can provide the same isoelectric point.
3 roteins was a substantially higher predicted isoelectric point.
4 separate bacteria by their surface charge or isoelectric point.
5 ntration and separation according to peptide isoelectric point.
6 eir primary structure, molecular weight, and isoelectric point.
7 e interfacial layer also increased below the isoelectric point.
8 culated molecular mass of 33 kDa and a basic isoelectric point.
9 acid of proteins, which causes the change in isoelectric point.
10 fer energy, (iv) beta turn frequency and (v) isoelectric point.
11 same monoisotopic masses but with differing isoelectric points.
12 tGPAT(-/-) liver mitochondria have different isoelectric points.
13 isoforms of AUF1 which migrated at multiple isoelectric points.
14 different phosphorylated forms with distinct isoelectric points.
15 progressively greater pH values exceed their isoelectric points.
16 hip between their order of elution and their isoelectric points.
17 having a wide range of molecular weights and isoelectric points.
18 forms, several of which differ only in their isoelectric points.
19 agnitude and separated on the basis of their isoelectric points.
20 , exist as two distinct forms with different isoelectric points.
24 (TREN)-ferritin adducts include an increased isoelectric point, a shift in the Cr(TREN) UV/vis spectr
25 remains bound to a peptide of 17 kDa with an isoelectric point above 9, demonstrating that His(176) i
26 hat contains the predicted molecular weight, isoelectric point, accession number, and putative functi
27 ry peak of expression of genes encoding high-isoelectric point alpha-amylase during grain development
28 s association is of sufficient power to make isoelectric point an excellent predictor of spliceosomal
29 lex peptide mixtures on the basis of peptide isoelectric point and (2) the introduction of peptide pI
31 ptide mixtures based on their differences in isoelectric point and hydrophobicity but also eliminates
33 liana were purified based on native mass and isoelectric point and identified by mass spectrometry.
34 ne amelogenin ("rM179") was minimum near its isoelectric point and increased rapidly below and above,
36 de gel electrophoresis (2-D PAGE) in similar isoelectric point and molecular mass ranges as studied b
37 ing of species-specific proteins, as well as isoelectric point and molecular weight sorting to facili
38 curated proteomes differ strongly in average isoelectric point and protein size, as well as transmemb
39 easing pH and strong agglomeration above the isoelectric point and settling in the presence of Ca.
42 emonstrated with amidation to modify protein isoelectric point and through derivatization with quater
43 ite a difference of 5 pH units between their isoelectric points and despite displaying only 16% seque
44 ncated 25- to 27-kDa isoforms having various isoelectric points and electrophoretic mobilities were e
45 utralization breadth have relatively neutral isoelectric points and preferentially bind to envelope m
46 are consistently distinguished by more basic isoelectric points and specificity for epitopes shared b
47 ire devices can be used to determine rapidly isoelectric points and variations in receptor-virus bind
48 erms or motifs, or by limiting the range for isoelectric points and/or molecular weights and links to
49 structural equivalence with respect to mass, isoelectric point, and recognition by conformation-sensi
50 imilar to synaptopodin in terms of its size, isoelectric point, and sequence although synaptopodin is
51 tion of the high net charge, due to the high isoelectric point, and the relatively low intrinsic hydr
53 explains the surprising observation that the isoelectric point appeared at approximately 3.0 when HCl
55 anogen bromide fragmentation, and calculated isoelectric point are all consistent with those determin
56 emistry and proteomics methods depend on the isoelectric point as a principal feature for protein and
58 se of solution pH from 5.0 to 6.0 toward the isoelectric point as well as more rapid dissociation of
60 Subsequently, we added estimates of protein isoelectric points as a feature to ANNs that classify sp
62 th information on predicted protein size and isoelectric point, as well as any repeats, motifs or dom
70 a 26-kDa polypeptide that differed in their isoelectric points between the C57BL/6J and the DBA/2J i
72 Moreover, NTHi P5 has a high theoretical isoelectric point, calculated as 9.58, which is not in a
73 ne adjustment of the buffer pH, close to the isoelectric point, can be used to slow down the transloc
74 c system-for example protein, DNA or RNA-the isoelectric point-commonly referred to as the pI-can be
75 d is likely facilitated by its difference in isoelectric point compared to the other VP sequences ass
76 eptides provided much faster measurements of isoelectric points compared with conventional isoelectri
78 ted on the basis of close proximity of their isoelectric points: D. chrysanthemi, D. chrysanthemi bv.
81 assay was applied to several mAbs of varying isoelectric points, demonstrating the suitability of Nan
82 ch is not in agreement with the experimental isoelectric point, determined as 6.3-6.8, or with its pr
83 ain complementarity determining region (CDR) isoelectric points did not correlate with silencing acti
84 o proteins with low molecular weight and low isoelectric points, distinguished fresh fillets from fro
85 kDa, WGA-reactive glycoprotein with a basic isoelectric point, distinguishing it from the acidic gly
86 y of the sample protein is reduced above the isoelectric point due to the decreased electrostatic att
87 on electrostatic attraction between the low-isoelectric-point enzyme and the microstructure, fabrica
88 of each protein such as molecular weight and isoelectric point, experimentally determined properties
89 somes have unique features (e.g., very basic isoelectric points, expressing the mutated tumor antigen
90 gical properties of MTases: structural fold, isoelectric point, expression pattern and cellular local
92 pproximately 8.5 was found correspond to the isoelectric point for the prepared sol-gel ODS coatings.
93 , we found inducible oligomers and shifts in isoelectric points for peroxiredoxin 1 (Prdx-1), Prdx-3,
94 ds; P22 Xis, 116 amino acids) and have basic isoelectric points (for P22 Xis, 9.42; for lambda Xis, 1
95 asic protein (MBP1) is an exceedingly basic (isoelectric point >11) 14-kDa protein, comprising the co
96 parameters, such as crosslinking density and isoelectric point, have been tuned in order to optimize
97 fold more "extreme" proteins (low mass, high isoelectric point, hydrophobic) than previous mitochondr
100 edicted molecular weight, (ii) the predicted isoelectric point, (iii) the accession number, and (iv)
102 and also the effectiveness of using peptide isoelectric point in conjunction with peptide match prob
103 resumably normosialylated based on a neutral isoelectric point) in other glomerular diseases as well.
104 ial dilatational rheology was highest at the isoelectric point, indicating the dominant role of the c
105 y extracting peptides, on the basis of their isoelectric points, into an immiscible organic phase fro
108 he overcharging of the complex away from its isoelectric point is caused by changes of the bulk struc
110 ature ZP3, with respect to both mol. wt. and isoelectric point, is partly a consequence of the N-link
111 aphid proteins were expressed as heritable, isoelectric point isoform pairs, one derived from each p
113 t to purification yields, thermal stability, isoelectric point, molar absorption coefficient, and eff
114 o acids in different physicochemical groups, isoelectric point, molecular weight, and length of prote
115 istribution of protein properties, including isoelectric point, molecular weight, number of exons, In
117 cognized a Mr 98,000 wall peroxidase with an isoelectric point near 4.2, and mWP19 recognized a Mr 58
119 -A, with a molecular mass of 69.4 kDa and an isoelectric point of 3.5 was purified from papaya latex.
120 l primary sequence of MARCKS with an overall isoelectric point of 4.2 yet a very basic PSD (overall c
122 It has a molecular weight of 63,000 and an isoelectric point of 4.6, and its hemolytic activity is
133 a 116-amino acid peptide of 13.5 kDa with an isoelectric point of 5.3 (residues 6-121), and His(176)
134 as an apparent molecular weight of 46,500 an isoelectric point of 5.5, and its pH optimum lies betwee
146 predicted molecular weight of 24,444 and an isoelectric point of 7.7 and lacked the ability to form
151 otease IV has a molecular mass of 26 kDa, an isoelectric point of 8.70, and optimum enzymatic activit
154 ed 12 transmembrane helices and predicted an isoelectric point of 9.4, both of which are characterist
155 , a predicted molecular mass of 9,647 Da, an isoelectric point of 9.81, and 90% to 95% sequence ident
156 mL) was treated with ammonium sulfate at the isoelectric point of alpha-Cbtx, and the pellet was diss
157 ein expression but did induce changes in the isoelectric point of Bim(EL) and its reactivity with an
158 H 7 as bulk water), a value that matches the isoelectric point of bubbles and oil droplets in indepen
163 s, TPT could change the molecular weight and isoelectric point of effector proteins, induce their met
164 O2 deprivation resulted in a decrease in the isoelectric point of eIF4E, consistent with additional p
167 significant shift toward lower values of the isoelectric point of maghemite upon selenium(IV) uptake,
169 ecific 50-kilodalton nuclear protein with an isoelectric point of pH 6.0 detected in 92% (59 of 64) o
170 n purified approximately 16,000-fold, has an isoelectric point of pH 6.1 and yields three catalytical
173 of the GPI-anchor to the molecular mass and isoelectric point of PrP quasispecies under two-dimensio
174 astase also reduced the size and changed the isoelectric point of the A(2) peptide, as determined by
177 evices also allowed for determination of the isoelectric point of the minute amounts of proteins immo
178 on procedure based on the molecular size and isoelectric point of the photoradiolabeled binding prote
179 ble to aggregation at pH values close to the isoelectric point of the protein (pH 4 and 5), at high i
180 e in charge density on the bead surface, the isoelectric point of the protein then dominates the mobi
181 oint for folding and only slightly below the isoelectric point of the protein, both the single domain
188 lution isoelectric focusing to determine the isoelectric point of Wnt3A protein in conditioned medium
190 t of native Hs11S was about 150-300 kDa with isoelectric points of 5.0-5.3, composed by four monomers
193 molecular weight of around 7.3 kDa, a basic isoelectric points of 8.5 and the presence of typical CX
194 ifications contribute to the measured acidic isoelectric points of Cx26 and Cx32, whereas their low m
195 ints of the homomeric channels bracketed the isoelectric points of heteromeric Cx26/Cx32 channels.
196 the first report of the determination of the isoelectric points of individual mitochondria by capilla
198 In this study, size, zeta-potential, and the isoelectric points of nanoparticles of the recombinant f
199 embly that correlated with a decrease in the isoelectric points of NCs, suggesting that the basic nat
200 rus transmission-competent F2 genotypes, the isoelectric points of the Buchnera proteins did not matc
204 t the variable amino terminus determined the isoelectric points of the TnT isoforms expressed, and th
205 at are in good agreement with the respective isoelectric points of Tim9 and Tim10 were determined.
207 re stable, reproducibly displayed a shift in isoelectric point, or changed in relative abundance at s
208 eptides of both hydrolysates showed that low isoelectric point peptides had antioxidant activity; how
211 re unstable to flocculation near the protein isoelectric point (pH 5.0), at high ionic strength (>100
215 eting CD25 [anti-Tac(scFv)-PE38] lowered its isoelectric point (pI) and decreased its toxicity in mic
216 ranslational modifications (PTMs) on protein isoelectric point (pI) and molecular weight and displays
217 proteins was affected by differences in the isoelectric point (pI) and the ion exchange properties o
218 employs the use of accurate mass and peptide isoelectric point (pI) as identification criteria, and r
220 f integrated microfluidic platforms for fast isoelectric point (pI) determinations via free-flow elec
222 otoheterotrophic life style and a low median isoelectric point (pI) for all predicted proteins, sugge
224 se/direct IAP binding protein with low pH of isoelectric point (pI) from the mitochondria, and the ac
226 ble minor structural protein (ORF3), with an isoelectric point (pI) of 10.0 and a calculated molecula
232 rameters in the Protein Data Bank (PDB), the isoelectric point (pI) of a protein has been explored as
233 The experimental amino acid composition and isoelectric point (pI) of Fap1 were similar to that pred
235 value of pH approximately one unit below the isoelectric point (pI) of the protein, at a concentratio
236 f the buffer is merely one pH unit below the isoelectric point (pI) of the recombinant proteins, most
238 contribution was ruled out by comparing the isoelectric point (pI) values to the adsorption of prote
239 ntibodies, to determine sCD44 concentration, isoelectric point (pI), and phosphorylation, respectivel
240 ome key parameters, such as applied voltage, isoelectric point (pI), bulk pH, and bulk conductivity,
247 hree fractions: one containing proteins with isoelectric points (pI values) higher than the pI of alb
248 lted in a cluster of ill-resolved peaks with isoelectric points (pI values) in the range 7.4 to 8.5.
249 n capabilities, using compounds with varying isoelectric points (pI) and pK(a) values over a range of
250 population was observed to exhibit distinct isoelectric points (pI) and, thus, distinct formal elect
251 wed differently charged 3C isoforms that had isoelectric points (pI) of 8.3 (55%) and 9.0 (45%), with
253 mn imaging detection (CIEF-WCID) method, the isoelectric points (pI) of complete intact polioviruses
254 ly discretized into fractions based on their isoelectric points (pI) without the use of carrier ampho
255 ion of acidic peptides (i.e., those with low isoelectric points (pI)) by matrix-assisted laser desorp
256 rogeneous organelles that likely have unique isoelectric points (pI), which are related to their surf
257 Analysis of hybrid CPs predicted that the isoelectric points (pI):charge value was 5.31:-2 for wil
258 ins (molecular mass [M(r)] 56 and 59 kDa and isoelectric point [pI] 5.2 and 5.3) in some, but not all
259 roteins in surface seawater exhibit alkaline isoelectric points (pIs of 8.10 and 8.37) and also acid
260 de mixtures based on the difference in their isoelectric points (pIs) after methyl esterification.
262 aganeite can be explained both by the higher isoelectric point/point-of-zero charge (9.6-10) of this
266 laminosum and found to be identical in mass, isoelectric point, redox midpoint potential and (for pla
268 rley and wheat were found to differ in their isoelectric points, resistance to proteolysis, and conta
270 PEG at a pH that was close to the protein's isoelectric point resulted in rapid nucleation and growt
271 is shows that individual parameters, such as isoelectric point, sequence length, average hydropathy,
272 iently phosphorylated by PKR in vitro and an isoelectric point shift in B56alpha was detected in extr
273 AhRY9F mutant and wild-type AhR, but a basic isoelectric point shift was detected by two-dimensional
274 ed for polymers, by linking photolysis to an isoelectric point shift, which itself is linked to a sol
275 rizes RA-binding proteins according to their isoelectric point showed both CRABP I and CRABP II to be
277 of apoptosis (IAP)-binding protein with low isoelectric point (Smac/DIABLO), and apoptosis-initiatin
278 to have an ER retention signal and an acidic isoelectric point, suggesting that it may be localized t
280 surface varies, and if it is lower than the isoelectric point, that factor increases (rectification
284 nding protein distinguishable by its neutral isoelectric point; the same binding protein was also det
285 L-FABP) is composed of isoforms differing in isoelectric point, their comparative structure and funct
286 and outer membrane proteins that have a high isoelectric point, thereby reducing their proton permeab
287 e of molecular mass, hydrodynamic radii, and isoelectric points through a nanofiber hydrogel scaffold
288 ated characteristics of biomolecules such as isoelectric points, titration curves and energies of pro
289 s of a sequence (e.g. surface composition or isoelectric point) to whole-genome data (e.g. absolute m
290 del peptides differing in hydrophobicity and isoelectric point using a combination of ion-exchange an
291 n of tubulin isotypes having a difference in isoelectric point values of 0.01, without the need for t
292 predicted molecular mass was 84 kDa, and the isoelectric point was 6.69, in agreement with rat brain
295 olyacrylamide gel electrophoresis, and their isoelectric points were determined to be greater than pH
299 roteins with different molecular weights and isoelectric points when a low-femtomole amount was loade
300 log, and stationary growth phases differ in isoelectric point, with values ranging from 5.2 to 6.4.
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