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1 tes increases with increasing valency of the isolectin.
2 high specificity for the xenograft antigen, isolectin 1-B(4) from Griffonia simplicifolia (GS-1-B(4)
3 ional lattice is formed from fucose-specific isolectin A from Lotus tetragonolobus cross-linked with
4 ng the gene encoding for the germ agglutinin isolectin A protein (Tri a 18 allergen), using the fluor
8 in the rat forebrain was characterized by B4 isolectin and Fluoro-Jade labeling techniques after 4 do
9 lial markers, as determined by staining with isolectin and for the endothelial-specific protein plate
10 At P17, retinal flatmounts were stained with isolectin and quantified with a standard protocol to mea
11 n Matrigel culture, followed by formation of isolectin and von Willebrand Factor-expressing cells, si
12 eriments carried out using 125I-labeled GS-I isolectins and type A human erythrocytes allowed us to d
15 populations, which were visualized using the isolectin B-4 and thiamine monophosphatase, were severel
16 jugates of cholera toxin B subunit (CTB) and isolectin-B(4) of Bandeiraea simplicifolia (IB(4)), were
18 DRG coexpressed Nav1.8 mRNA (84%) and bound isolectin B4 (72%), relatively few GFP profiles were pos
19 ous injection of the Griffonia simplicifolia isolectin B4 (IB4) at 1-28 days following a moderate tho
20 by cell size, action potential duration and isolectin B4 (IB4) binding] generated distinct responses
21 subunit B (CTB) for myelinated A fibers and isolectin B4 (IB4) for unmyelinated C fibers and both la
25 erve growth factor dependent, binding either isolectin B4 (IB4) or antibodies to calcitonin gene-rela
26 erivascular markers such as Evans blue (EB), isolectin B4 (IB4) or laminin (LN) are used alongside si
27 ified afferents revealed that most CPMs were isolectin B4 (IB4) positive and transient receptor poten
31 xclusively expressed in afferents binding to isolectin B4 (IB4), a neurochemical marker of non-peptid
32 In contrast, preventing sensory input from isolectin B4 (IB4)-binding fibers blocked movement-induc
34 sistant (TTX-R) Na+ channel currents between isolectin B4 (IB4)-positive and IB4-negative small DRG n
35 r calcitonin gene-related protein (CGRP) and isolectin B4 (IB4)-positive neurons in the L4 and L5 ips
40 cluding concanavalin A (conA), Vicia villosa isolectin B4 (VVL-B(4)), and Ricinus communis agglutinin
42 ncta immunopositive for GluR2/4 costain with isolectin B4 after injections of these tracers in the sc
43 rons that appear after inflammation also had isolectin B4 binding, suggesting that some mechanorecept
46 predominantly colabeled with peripherin and isolectin B4 markers of unmyelinated C-fiber neurons; 68
47 in-eosin, Masson trichrome, and biotinylated isolectin B4 stains were used to assess regional vascula
48 ome and Biotinylated Bandeiria simplicifolia Isolectin B4 stains were used to characterize scarred my
52 e number of double-positive cells (beta-gal, isolectin B4) on the luminal surface in carotid arteries
54 bone marrow origin and Tie-2 expression, and isolectin B4, also indicating endothelial lineage, in th
55 The majority of EGFP-positive neurons bound isolectin B4, although a small percentage ( approximatel
56 I (BS-I), the wholly alpha-gal-specific BS-I isolectin B4, and elicited primate anti-pig xenoreactive
57 led with calcitonin gene-related peptide and isolectin B4, and injured unmyelinated afferents labeled
58 with expression of the surface markers LA4, isolectin B4, and LD2, kainate receptors are present on
59 of primary afferent nociceptors, peripherin, isolectin B4, and substance P, and markers of myelinated
61 ained for calcitonin gene-related peptide or isolectin B4, but none of these was immunopositive for s
62 c DRG cells with ADPs stained positively for isolectin B4, but only diabetic cells responded robustly
63 tem examination, tissue samples stained with isolectin B4, Masson trichrome, and hematoxylin-eosin we
66 ing AMPH exposure was quantified by counting isolectin B4-labeled phagocytic microglia and Fluoro-Jad
69 rize a novel subpopulation of capsaicin- and isolectin B4-positive nociceptors that also expresses a
79 d with fluorescein isothiocyanate-conjugated isolectin-B4 for endothelium to demarcate vascular struc
80 graphy (FA), confocal volumetric analysis of isolectin-B4 labeled flatmounts, and histologic examinat
81 vascular patterns consistent with ischemia; isolectin-B4 labeling revealed a capillary-free zone cen
82 rescence microscopy to detect blood vessels (isolectin-B4), angiogenesis [anti-vascular endothelial g
83 scence microscopy to visualize vessels using isolectin-B4, to detect angiogenesis using anti-VEGF and
85 orm cortex of the younger rats, extensive B4 isolectin binding to activated microglia was observed in
87 and (2) the association constant of the GS-I isolectins for human type A erythrocytes increases with
88 the increased affinity displayed by the GS-I isolectins for human type A erythrocytes is dependent on
89 MS sequencing confirmed the presence of two isolectin forms in commercially available WFA differing
90 y means of lectin histochemistry with the B4-isolectin from Griffonia simplicifolia as well as immuno
91 putative markers of nociceptive DRG neurons, isolectin Griffonia simplicifolia (I-B4), identifying sm
92 as were isolated, blood vessels stained with isolectin Griffonia simplicifolia, images of retinal who
94 f astrocytes, (b) griffonia simplicifilia B4-isolectin (GSA-IB4) horse radish peroxidase (HRP)-conjug
98 nst macrophages) and Griffonia simplicifolia isolectin IB(4), and stain intensely for the lysosomal p
99 RPE/choroid flatmounts were labeled with isolectin IB4 to determine CNV lesion volumes using conf
100 -diamino-2-phenylindole), endothelial cells (isolectin IB4), microglia (CD11b), and filamentous actin
101 SP), somatostatin (SOM) immunoreactivity and isolectin IB4, and cholera toxin B (ChTB) binding were e
105 Whole mounts of retinas were prepared for isolectin immunohistochemistry, and preretinal or intrav
108 ser injury, phalloidin-labeled RPE cells and isolectin-labeled endothelial cells increased significan
109 Codistribution of Galphat-S-ir fibers and isolectin-labeled extrabulbar primary olfactory fibers w
110 enesis was performed using barium sulfate or isolectin microangiography and Doppler ultrasonography o
111 ate that (1) the association constant of the isolectin monovalent for alpha-D-GalNAc (AB3) is virtual
112 njugates of choleragenoid (B-HRP) and the B4 isolectin of Bandeiraea simplicifolia (IB4-HRP) on oppos
114 tran; immunohistochemistry with biotinylated isolectin, rabbit anti-NG2, rat anti-CD31, rabbit anti-V
117 on in the mouse OIR model based on images of isolectin-stained retinal wholemounts, we were able to a
118 perfusion and elastin immunohistochemistry, isolectin staining, and confocal fluorescence microscopy
119 th 20 microg/ml Griffonia simplicifolia (GS) isolectin to identify microglia and define their morphol
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