ライフサイエンスコーパス: isopeptidase

1 mer of benenodin-1 is cleaved by its cognate isopeptidase.

2 defects of cells lacking the divergent Ulp2 isopeptidase.

3 peptides except for one were cleaved by the isopeptidase.

4 tin binding subunit, and dUCH37, a ubiquitin isopeptidase.

5 is serving as a recognition element for the isopeptidase.

6 identified it as an allele of the ULP2 SUMO isopeptidase.

7 bal), an inhibitor of many ubiquitin-protein isopeptidases.

8 and cleaved by enzymes called lasso peptide isopeptidases.

9 determined at the level of deconjugation by isopeptidases.

10 /RanBP2 that preferentially protects it from isopeptidases.

11 disassembled slowly by proteasome-associated isopeptidases.

12 have both deubiquitinating and deISGylating isopeptidase activities in addition to proteolytic activ

13 All three enzymes were found to display good isopeptidase activities, with Km values of 10(-4) to 10(

14 olog, has SUMO C-terminal hydrolase and SUMO isopeptidase activities.

15 ere detected; notably, in addition to UCH-D, isopeptidase activity [ubiquitin-(epsilonN)-lysine cleav

16 We demonstrate isopeptidase activity against K48-linked tetraubiquitin

17 In vivo, SENP5 showed isopeptidase activity against SUMO-2 and SUMO-3 conjugat

18 terochromatin protein 1 alpha (HP1alpha) and isopeptidase activity against SUMO-modified HP1alpha.

19 ere then assayed for inhibition of ubiquitin isopeptidase activity and antineoplastic effects.

20 The punaglandins were shown to inhibit isopeptidase activity and exhibit antiproliferative effe

21 been proposed to provide the active site for isopeptidase activity associated with the Rpn11/POH1 sub

22 Transgenic inhibition of CSN5 isopeptidase activity blocks breast cancer progression e

23 e other SENP family members, SENP7S has SUMO isopeptidase activity but unlike full-length SENP7L, SEN

24 proNEDD8 precursor to its mature form and an isopeptidase activity deconjugating NEDD8 from substrate

25 y the enzymes, indicating that expression of isopeptidase activity did not require unusual protein co

26 These results highlight CSN5 isopeptidase activity in breast cancer progression, sugg

27 the use of gamma-glutamyltranspeptidase and isopeptidase activity in leech saliva, we could show tha

28 that Uch37 is responsible for the ubiquitin isopeptidase activity in the PA700 (19S) proteasome regu

29 Furthermore, SENP5 showed more limited isopeptidase activity in vitro.

30 Here, we show that CSN5 isopeptidase activity is essential for breast epithelial

31 ssembly of the full COP9 signalosome and the isopeptidase activity of CSN5, which potentiates the tra

32 e discovered that they inhibit the ubiquitin isopeptidase activity of the proteasome pathway.

33 as shown to preferentially inhibit ubiquitin isopeptidase activity of the proteasome pathway.

34 emoval of polyubiquitin moieties through the isopeptidase activity of the subunit Rpn11.

35 physically binds SUMO and displays in vitro isopeptidase activity on poly-SUMO chains.

36 y regulates IFN signaling independent of its isopeptidase activity towards ISG15.

37 ) and removing conjugated SUMO from targets (isopeptidase activity).

38 Whereas CSN-dependent CSN5 displays isopeptidase activity, it is intrinsically inactive in o

39 nsaturated ketone, was a potent inhibitor of isopeptidase activity, whereas PGA(1) and PGA(2) with si

40 n5 subunit was found to underlie CSN's Nedd8 isopeptidase activity.

41 reover, the centrosomes contain an ubiquitin isopeptidase activity.

42 hese peptides is dispensable with regards to isopeptidase activity.

43 ght-subunit protein complex containing Nedd8 isopeptidase activity.

44 Thus, SENP5 also possesses limited SUMO-1 isopeptidase activity.

45 ect of USP13 on Siah2 is not mediated by its isopeptidase activity: mutations in its ubiquitin-bindin

46 f small, ubiquitin-related modifier-specific isopeptidases (also known as sentrin-specific proteases,

47 racts with the endosome-associated ubiquitin isopeptidase AMSH, and the coexpression of AMSH or its C

48 how the opposing actions of a localized SUMO isopeptidase and a STUbL regulate rDNA silencing by cont

49 UBP43 belongs to the family of ubiquitin isopeptidases and specifically cleaves ISG15, a ubiquiti

50 e pharmacophore hypothesis, inhibit cellular isopeptidases, and cause cell death independently of p53

51 ns is challenging because of the activity of isopeptidases, and often only a small fraction of a targ

52 We also use this isopeptidase as a tool to study evolutionary relationshi

53 Furthermore, it identifies isopeptidases as novel targets for the development of an

54 We identified SMT4, which encodes a SUMO isopeptidase, as a high copy suppressor of both the temp

55 The method simplifies the isopeptidase assay and facilitates high-throughput analy

56 cture-activity analysis of the lasso peptide isopeptidase AtxE2 from Asticcacaulis excentricus, we so

57 only the distal end of (poly)Ub chains, this isopeptidase can selectively rescue poorly ubiquitinated

58 inhibitors, and that inhibitors of ubiquitin isopeptidases cause cell death in vitro independently of

59 ession of SENP2, but not other SUMO-specific isopeptidases, causes a defect in chromosome congression

60 tivity was intrinsic to PA700 and the Brcc36 isopeptidase complex (BRISC), but that the CSN-associate

61 A1-RAP80 complex, and the cytoplasmic BRCC36 isopeptidase complex (BRISC).

62 BRISC (Brcc36-containing isopeptidase complex) is a four-subunit deubiquitinating

63 Potentially, an isopeptidase could influence degradation by 'editing' (p

64 8 and Ile44 in the distal-end Ub contact the isopeptidase directly.

65 PA700 isopeptidase disassembles Lys 48-linked polyubiquitin sp

66 and by a mutation in a proteasome-associated isopeptidase (doa4).

67 or Nup358/RanBP2 binding, or by manipulating isopeptidase expression levels, paralog-selective modifi

68 The isopeptidase gene can be used to facilitate genome minin

69 peptide biosynthetic enzymes, lasso peptide isopeptidase has broad substrate specificity.

70 2) SUMO isopeptidases have little substrate specificity.

71 ates; and (iv) distinct ubiquitin C-terminal isopeptidase/hydrolase activities, including potent ubiq

72 The specificity of the ubiquitin (Ub) isopeptidase in the PA700 regulatory complex of the bovi

73 ates, validating that USP18 is a major ISG15 isopeptidase in vivo.

74 ively little is known about the role of SUMO isopeptidases in genome maintenance and their role in co

75 that specificity determinants for the PA700 isopeptidase include Leu8, Ile44, and Lys48 on the dista

76 shown to exert its regulatory function in an isopeptidase-independent manner.

77 It is theorized that isopeptidase inhibition and general cytotoxicity of pros

78 nes structurally related to the nonselective isopeptidase inhibitor G5 were synthesized and tested fo

79 However, G5, a non-selective isopeptidase inhibitor, triggers a distinct necrotic pat

80 ression vector after which ubiquitin-protein isopeptidase inhibitor, Ubal, and expression vector were

81 nfers activity among this class of ubiquitin isopeptidases inhibitors, and that inhibitors of ubiquit

82 Lasso peptide isopeptidase is an enzyme that specifically hydrolyzes t

83 his latter possibility we find that the WSS1 isopeptidase is required for suppression by ulp2Delta.

84 identity and 54.8% amino acid identity to n Isopeptidase (ISOT-1).

85 nthreaded astexin-2, demonstrating that this isopeptidase must recognize a knotted structure in order

86 a high copy suppressor selection with a SUMO isopeptidase mutant, and tandem mass spectrometry to def

87 d electrophilic beta-carbons, do not inhibit isopeptidases or cause significant cell death.

88 Generic inhibition of SUMO isopeptidases or depletion of the SUMO isopeptidase SENP

89 ubiquitin recycling by proteasome-associated isopeptidases, our results indicate that ubiquitin is re

90 We propose that JAMM isopeptidases play important roles in a variety of physi

91 Ub aldehyde (Ubal), a synthetic inhibitor of isopeptidases (proteases that hydrolyze the bond between

92 .4 A resolution structure of a lasso peptide isopeptidase revealing a topologically novel didomain ar

93 and also occurs if Ub aldehyde occupies this isopeptidase's active site.

94 es, it can be disassembled to Ub monomers by isopeptidase(s) in a red blood cell extract.

95 lpha-subunit proteolysis by inhibition of an isopeptidase(s) that deubiquitinates, or "edits," Ub-3H-

96 by the 26S proteasome; and disassembly by Ub isopeptidase(s) to regenerate the protein substrate.

97 High Ubal concentrations inhibit an isopeptidase(s) which normally disassembles "unanchored"

98 SUMO-specific isopeptidase SENP-1 further enhances the synergy between

99 ng GluR6 SUMOylation using the SUMO-specific isopeptidase SENP-1 prevents kainate-evoked endocytosis

100 d, mutating these lysines or adding the SUMO isopeptidase SENP1 dramatically increased both native an

101 SUMO isopeptidases or depletion of the SUMO isopeptidase SENP1 enhances sumoylation of Ran in semi-p

102 l ubiquitin-related modifier (SUMO)-specific isopeptidases SENP1 and SENP2 are targeted to kinetochor

103 y regulate SAC proteostasis through the SUMO-isopeptidases SENP1 and SENP2 at NPCs.

104 in excised PM patches are activated by SUMO isopeptidase (SENP1) and resilenced by SUMO1.

105 ligases (UBA2 and Ubc9) or the sumo specific isopeptidases (SenP1-3).

106 ysiologically significant constraint on SUMO isopeptidase specificity.

107 at is sufficient to impose on CSN5 an active isopeptidase state.

108 Isopeptidase T (IPaseT) can hydrolyze isopeptide bonds o

109 abbit reticulocyte deubiquitinating enzymes: isopeptidase T (IPaseT), a member of the gene family of

110 Unlike the BUZ domain from isopeptidase T (isoT) that contains a single zinc finger

111 ubstrate preferences of two DUBs, UCH-L3 and isopeptidase T (IsoT), were profiled using a positional

112 subunit of G proteins (GNB3), and ubiquitin isopeptidase T (ISOT), with known functions, and two new

113 n (ZnF UBP) from the deubiquitinating enzyme isopeptidase T (IsoT, or USP5) alone and in complex with

114 Isopeptidase T (IsoT/USP5) is a deubiquitinating enzyme

115 s stable and tightly bound recombinant human Isopeptidase T (USP5), a deubiquitinating enzyme known t

116 ion, e.g., the deubiquitinating enzymes USP5/isopeptidase T and USP7/HAUSP and the ubiquitin ligases

117 Finally, Ubp14 and human isopeptidase T are shown to be functional homologs by co

118 TTN6 is related to the isopeptidase T class of deubiquitinating enzymes that re

119 ic tri-Ub resists hydrolysis by the PA700 or isopeptidase T deubiquitinating enzymes, it can be disas

120 We propose that Ubp14 and isopeptidase T facilitate proteolysis in vivo by prevent

121 A novel Isopeptidase T gene (ISOT-3) has been identified on huma

122 iculocyte lysate are specific substrates for isopeptidase T in lysates.

123 In the presence of 0.5 microM ubiquitin, isopeptidase T was inhibited by several of the dimer ana

124 In the absence of ubiquitin, isopeptidase T was inhibited only by the dimer linked th

125 Isopeptidase T was shown to specifically disassemble two

126 ockdown of the deubiquitinating enzyme USP5 (isopeptidase T) results in an increase in the level and

127 e identified as Ubp14, the yeast ortholog of Isopeptidase T, and Ufd3, a member of the ubiquitin-fusi

128 binds to ubiquitin-conjugating enzyme-9 and isopeptidase T-3, enzymes involved in small ubiquitin-re

129 conjugates was similar to that observed for isopeptidase T.

130 in vitro, a specificity shared by mammalian isopeptidase T.

131 The Ub-specific protease isopeptidase T/USP5 is shown to react with ISG15-VS.

132 The ZnF UBP domain of isopeptidase-T showed no linkage specificity for poly-Ub

133 eas SENP2 and -5-7 have substantially higher isopeptidase than endopeptidase activities.

134 esults suggest that Wss1 is a SUMO-dependent isopeptidase that acts on sumoylated substrates as they

135 Here we describe a bovine isopeptidase that is well suited to such an editing func

136 phogenesis 9 (COP9) signalosome 5 (CSN5), an isopeptidase that removes neural precursor cell-expresse

137 that this enzyme, AtxE2, is a lasso peptide isopeptidase that specifically hydrolyzes astexins-2 and

138 NAi analysis identified three NEDD8-specific isopeptidases that, when knocked down, suppress apoptosi

139 Ubp43 is an ISG15-specific isopeptidase, the expression of which is activated by IF

140 Isopeptidases, the enzymes that reverse the actions of C

141 Thus, protection from isopeptidases, through interactions with SUMO-binding pr

142 dopeptidase to process the pre-SUMO or as an isopeptidase to deconjugate SUMO from its substrate.

143 ouse model that is lacking an ISG15-specific isopeptidase to study the biological role of the protein

144 pposing activities of Rsp5 and the ubiquitin isopeptidase Ubp2, which are known to assemble and disas

145 However, mutants lacking the SUMO-specific isopeptidase Ulp2 accumulated high molecular weight SUMO

146 Here we show the SUMO isopeptidase Ulp2 in Saccharomyces cerevisiae does not p

147 irement was observed for mutants of the SUMO isopeptidase Ulp2/Smt4.

148 ng actions of a STUbL (Slx5:Slx8) and a SUMO isopeptidase (Ulp2).

149 ISG15 modification is counteracted by the isopeptidase USP18, a major negative regulator of IFN si

150 The determinants recognized by the isopeptidase were probed by the use of mutant ubiquitins

151 ounteracted by de-ubiquitinating enzymes (or isopeptidases) which selectively remove the polyubiquiti

152 Unp is a human isopeptidase with still poorly understood biological fun

153 -conjugating enzyme UBE2E3 and the ubiquitin isopeptidase Y (UBPY).

154 ucing adaptor molecule)) and UBPY (ubiquitin isopeptidase Y) have opposite effects on epidermal growt