ライフサイエンスコーパス: isopeptide

1 is not rate-limiting for hydrolysis of this isopeptide.

2 breaking of Nepsilon-(gamma-glutamyl)lysine isopeptides.

3 g that the proteasome can produce sufficient isopeptide Ag to evoke a T cell response.

4 Finally, we show that isopeptide Ags are immunogenic in vivo.

5 be generally applicable to the synthesis of isopeptide analogues in which an oxirane replaces the sc

6 rked and dose-dependent inhibitory effect on isopeptide and ester bond formation.

7 We conclude that both isopeptide and ester bond ubiquitination regulate protea

8 w that ubiquitin is attached to hD4R through isopeptide and ester bonds.

9 ly catalyses the cleavage of ubiquitin-like (isopeptide and linear) bonds from target proteins.

10 in with the capacity to hydrolyze thioester, isopeptide, and peptide bonds.

11 njugates generated in H. volcanii as well as isopeptide- and linear-linked SAMP1-MoaE in purified for

12 nists of endothelin-1 (ET-1) and its related isopeptides are important tools defining the role of ET

13 Additionally, we show that isopeptides are more stable toward further proteasomal p

14 that catalyze the hydrolysis of peptides and isopeptides are often susceptible to inactivation by ele

15 talytically competent and compatible with di-isopeptide binding, the Ins-1 segment obstructs access t

16 Ub) carboxylate group either by a peptide or isopeptide bond abolishes BUZ-domain interaction.

17 produce PCNA-Ub at high yield with a native isopeptide bond and study its Usp1/UAF1-dependent deconj

18 A mutant lacking the CNA(1) isopeptide bond assembled deformed pilin subunits that f

19 threaded knot structure that is formed by an isopeptide bond attaching the N-terminus of the peptide

20 structure in these peptides derives from an isopeptide bond attaching the N-terminus of the peptide

21 yme forms an epsilon-(gamma-glutamyl) lysine isopeptide bond between a lysine donor from one protein

22 specificity and catalyze the formation of an isopeptide bond between a lysine residue of the substrat

23 on signal, and catalyzes the formation of an isopeptide bond between a substrate (or ubiquitin) lysin

24 ation activity that leads to formation of an isopeptide bond between glutamine and lysine residues fo

25 XIII (FXIIIa) catalyzes the formation of an isopeptide bond between glutamine at position two in alp

26 in FbaB contains a domain with a spontaneous isopeptide bond between Lys and Asp.

27 desulfurization to yield an entirely native isopeptide bond between substrate proteins and ubiquitin

28 polyubiquitin chain, which is defined by an isopeptide bond between the C terminus of one ubiquitin

29 in, monomers are linked to each other via an isopeptide bond between the C-terminal glycine of one Ub

30 pically thought to occur via formation of an isopeptide bond between the C-terminal glycine residue o

31 ovalently attached to a substrate through an isopeptide bond between the ubiquitin carboxy terminus a

32 G15 substrates, as well as in their rates of isopeptide bond cleavage of K48- and K63-linked polyubiq

33 The recent discovery of intramolecular isopeptide bond cross-links, formed autocatalytically, i

34 e active site, which exposes and orients the isopeptide bond for hydrolysis.

35 proposed to contain activities in catalyzing isopeptide bond formation (ubiquitin ligation) and subst

36 linkage of SpyTag shed light on spontaneous isopeptide bond formation and should provide a targetabl

37 In theory, polyubiquitination can occur by isopeptide bond formation at any of the seven lysine res

38 r proteins through a mechanism that involves isopeptide bond formation between Gln and Lys residues a

39 r proteins through a mechanism that involves isopeptide bond formation between Gln and Lys residues.

40 on-lysine transferase EC 2.3.2.13) catalyzes isopeptide bond formation between glutamine and lysine r

41 enzymatic protein cross-linking activity via isopeptide bond formation between glutamine and lysine r

42 antibody labeling approach which is based on isopeptide bond formation between two recognition peptid

43 Spontaneous isopeptide bond formation, a stabilizing posttranslation

44 or catalysis of E2- and E2/RING E3-dependent isopeptide bond formation, but dispensable for upstream

45 asparagine are dispensable for catalysis of isopeptide bond formation.

46 hat utilize distinct mechanisms in promoting isopeptide bond formation.

47 , that locks itself together via spontaneous isopeptide bond formation.

48 ilin monomer, which joins two pilins with an isopeptide bond formed between threonine and lysine.

49 the modification by specific cleavage of the isopeptide bond formed with Pup.

50 inked by way of the transglutaminase-induced isopeptide bond has not been reported.

51 ubunit is found approximately 2.4 A from the isopeptide bond in the partly hydrophobic pocket that co

52 TAM directed AMSH cleavage toward the distal isopeptide bond in tri-ubiquitin chains.

53 upport that (1) the cleavage of the proximal isopeptide bond is not a prerequisite for proteasomal de

54 onstrate that the Nepsilon-Gly-L-homothiaLys isopeptide bond is processed to a similar extent by deub

55 our finding that BRISC could not cleave the isopeptide bond joining a ubiquitin to a non-ubiquitin s

56 s an enzyme that specifically hydrolyzes the isopeptide bond of lasso peptides, rendering these pepti

57 demonstrated by isolating and sequencing the isopeptide bond of polyubiquitin.

58 ss, we systematically examined the effect of isopeptide bond position and molecular sizes of auxiliar

59 abilized by factor XIIIa through an amide or isopeptide bond that ligates adjacent fibrin monomers.

60 -Thr-Glu-Arg cross-linked via an Lys3-Gln102 isopeptide bond to Ala-Val-Pro-Ser-Gln102-Lys.

61 pathways that attach the polypeptides via an isopeptide bond to epsilon-lysyl amino group(s) in the t

62 s a peptide engineered to form a spontaneous isopeptide bond to its protein partner SpyCatcher.

63 n specificity and the mechanism to cleave an isopeptide bond to release m-DAP.

64 NF4 in complex with E2 (UbcH5A) linked by an isopeptide bond to ubiquitin.

65 r an enzyme that specifically hydrolyzes the isopeptide bond to yield the linear peptide.

66 g an aspartyl catalyst, did not generate the isopeptide bond within the jelly-roll structure of XNA.

67 ntermolecular epsilon-(gamma-glutamyl)lysine isopeptide bond(s) and the formation of high molecular m

68 rtners which lock together via a spontaneous isopeptide bond).

69 ns, each of which harbours an intramolecular isopeptide bond, previously described in several Gram-po

70 DUBs) as that of a native Nepsilon-Gly-L-Lys isopeptide bond, thereby establishing the utility of TEC

71 hrough mechanical unfolding and refolding of isopeptide bond-delimited polypeptide loops present in I

72 tide bond-linked polyubiquitin, but not with isopeptide bond-linked polyubiquitin, indicating that th

73 ine side chains of the target protein via an isopeptide bond.

74 sine side chain of a substrate protein by an isopeptide bond.

75 uorine substitution adjacent to the scissile isopeptide bond.

76 an electrophilic replacement of the scissile isopeptide bond.

77 most cases, a lysine of the substrate via an isopeptide bond.

78 proteases that specifically cleave only the isopeptide bond.

79 he Ub that donates the C-terminal Gly to the isopeptide bond.

80 n site" binds the Ub that donates Lys to the isopeptide bond.

81 developmentally downregulated gene 8 (Nedd8)isopeptide bond.

82 so remodel ubiquitin chains to hydrolyse the isopeptide bond.

83 strands are linked through an intramolecular isopeptide bond.

84 cceptor lysine, resulting in formation of an isopeptide bond.

85 ses (MTGs) catalyze the formation of Gln-Lys isopeptide bonds and are widely used for the cross-linki

86 ical studies demonstrate that although these isopeptide bonds are dispensable for fimbrial assembly,

87 These covalent isopeptide bonds are of great physiological significance

88 In a mixed chain, not all the isopeptide bonds are restricted to a specific lysine of

89 by incorporating site-specific interhelical isopeptide bonds as the redox-sensitive disulfide surrog

90 otein substrate occurred exclusively through isopeptide bonds at a lysine epsilon-amino group within

91 n which the 8.6-kDa polypeptide is linked by isopeptide bonds between carboxyl termini and Lys-48 res

92 Poly-Ub chains, linked through isopeptide bonds between internal Lys residues and the C

93 be ligated together through the formation of isopeptide bonds between Lys48 and Gly76 of successive u

94 Factor XIII catalyzes the formation of isopeptide bonds between noncovalently associated fibrin

95 s an autocatalytic reaction that creates 420 isopeptide bonds between proteins.

96 formation of epsilon-(gamma-glutamyl)lysine isopeptide bonds between specific Gln and Lys residues.

97 collision-induced dissociation demonstrated isopeptide bonds between the C-terminal glycine of SAMP2

98 moiety or a chain of Ub molecules joined by isopeptide bonds between the C-terminus of one Ub with o

99 for fiber formation as they create covalent isopeptide bonds between the sortase recognition motif a

100 et share a common beta-grasp fold, also form isopeptide bonds by a mechanism that appears streamlined

101 Removal of the isopeptide bonds by mutagenesis readily allowed Spy0128

102 s) are defined as enzymes capable of forming isopeptide bonds by transfer of an amine onto glutaminyl

103 ture of these reduction-insensitive bonds is isopeptide bonds formed between side chains of lysine an

104 of cross-linking of constituent proteins by isopeptide bonds formed by transglutaminases.

105 ach acquire intramolecular lysine-asparagine isopeptide bonds formed via catalytic glutamic acid or a

106 at mutants unable to form the intramolecular isopeptide bonds in the CNA(2) or CNA(3) domains retain

107 or studies have demonstrated the presence of isopeptide bonds in the sheath and cuticle of filarial p

108 HK97 subunits cross-link via isopeptide bonds into oligomers that are closed rings of

109 Cyclization via domains forming spontaneous isopeptide bonds is a general strategy to generate resil

110 dification where ubiquitin chains containing isopeptide bonds linking one of seven ubiquitin lysines

111 Isopeptidase T (IPaseT) can hydrolyze isopeptide bonds of polyubiquitin (polyUb) chains, simpl

112 Prokaryotes form ubiquitin (Ub)-like isopeptide bonds on the lysine residues of proteins by a

113 The chemically identical isopeptide bonds recently documented in bacterial pili h

114 rom the strategically located intramolecular isopeptide bonds recently identified in the x-ray struct

115 tin (Ub) chains linked through Lys-48-Gly-76 isopeptide bonds represent the principal signal by which

116 olypeptides covalently linked via peptide or isopeptide bonds to the C-terminal glycine of ubiquitin.

117 nds its protein partner SpyCatcher and forms isopeptide bonds under physiological conditions.

118 n chains linked internally via epsilon-amino isopeptide bonds using Lys48 and can process some, but n

119 cy-thiolactone underlies its ability to form isopeptide bonds with protein lysine residues (N-Hcy-pro

120 Hcy-thiolactone has the ability to form isopeptide bonds with protein lysine residues, which gen

121 Hcy-thiolactone has the ability to form isopeptide bonds with protein lysine residues, which gen

122 Hcy-thiolactone has the ability to form isopeptide bonds with protein lysine residues, which gen

123 oil proteins, in contrast to those linked by isopeptide bonds, are dispersed by treatment with chemic

124 olyubiquitin chain assembled through K48-G76 isopeptide bonds, rather than by ligation of monoubiquit

125 of substrates was thought to occur only via isopeptide bonds, typically to lysine residues.

126 including native ISG15 conjugates linked via isopeptide bonds.

127 was active in vivo based on the detection of isopeptide bonds.

128 establishing epsilon-(gamma-glutamyl)lysine isopeptide bonds.

129 in chains linked exclusively through K48-G76 isopeptide bonds.

130 omain molecule stabilized by two intradomain isopeptide bonds.

131 tin molecules are connected through specific isopeptide bonds.

132 We recently discovered that crosslinking isopeptide bridges within the de novo helical trimers ad

133 one of two minor components of the isolated isopeptides, but neither the major isopeptide nor the ot

134 are normal peptides, and we demonstrate that isopeptides can bind to HLA-A2.1 and HLA-A3 with high af

135 ly on the catalytic cysteine, abrogating the isopeptide-cleaving activity without affecting these enz

136 ns, it efficiently deubiquitinates a Ub-SUMO isopeptide conjugate and a Ub-SUMO fusion protein.

137 perties of the derivative bearing an A11-B10 isopeptide cross-link suggests that the disulfide has a

138 Microscopic localization and isopeptide cross-linking studies suggest that TIG3 and t

139 mmunohistochemical analysis, indicating that isopeptide cross-links are important for the stabilizati

140 This enzyme catalyzes the formation of isopeptide cross-links between fibrin molecules in nasce

141 Isopeptide cross-links form between asparagine and lysin

142 Furthermore, isopeptide cross-links were reduced in both uninjured sk

143 acellular matrix proteins by introduction of isopeptide cross-links.

144 (TGases) are enzymes that catalyze covalent isopeptide crosslinks between reactive lysine and glutam

145 om human epidermal cell envelopes containing isopeptide crosslinks inserted by transglutaminases in v

146 Because the U-II isopeptide family is highly conserved across species, bo

147 that recognizes diglycine (diGly)-containing isopeptides following trypsin digestion.

148 at specifically eliminates RING E3-catalyzed isopeptide formation but not HECT E3 transthiolation (N7

149 wo epoxide-containing peptidomimetics of the isopeptide, glutamyl-gamma-glutamate, have been synthesi

150 yme-product complex is rate-limiting in this isopeptide hydrolysis reaction.

151 nation and elicits greater ubiquitin-protein isopeptide ligase (E3) activity toward p53, thereby incr

152 owever, the mRNA levels of ubiquitin-protein isopeptide ligase (E3) carboxyl terminus of Hsp70-intera

153 the identification of the ubiquitin-protein isopeptide ligase (E3) EFP (estrogen-responsive finger p

154 We previously described a ubiquitin-protein isopeptide ligase (E3) from erythroid cells that assembl

155 in) and RING domains) is a ubiquitin-protein isopeptide ligase (E3) that is important for the control

156 The ubiquitin-protein isopeptide ligase (E3) that ubiquitinated IRF-8 was like

157 with Hsp40, Hsp70, and the ubiquitin-protein isopeptide ligase (E3) ubiquitin ligase carboxyl terminu

158 The SCF-ROC1 ubiquitin-protein isopeptide ligase (E3) ubiquitin ligase complex targets

159 ergy in lymphocytes) is an ubiquitin-protein isopeptide ligase (E3) ubiquitin ligase necessary for th

160 or for the SCFHOS/betaTrCP ubiquitin-protein isopeptide ligase (E3) via one canonical and one cryptic

161 Mutations of parkin, a protein-ubiquitin isopeptide ligase (E3), appear to be the most frequent c

162 unctions like an anti-SUMO ubiquitin-protein isopeptide ligase (E3), interacting both with HSF2 and t

163 CF(Skp2/Cks1) complex, the ubiquitin-protein isopeptide ligase (E3).

164 by the dipeptide Leu-Ala, an inhibitor of Ub isopeptide ligase (E3).

165 Parkin is a ubiquitin-protein isopeptide ligase able to translocate to the mitochondri

166 dTopors protein possesses ubiquitin-protein isopeptide ligase activity in vitro and that dTopors med

167 strate that San1 possesses ubiquitin-protein isopeptide ligase activity in vitro, and the ubiquitin-p

168 p90 substrates through the ubiquitin-protein isopeptide ligase activity of CHIP.

169 activity in vitro, and the ubiquitin-protein isopeptide ligase activity of San1 is required for its f

170 cting proteins through the ubiquitin-protein isopeptide ligase activity of their RING domain.

171 an IAPs by promoting their ubiquitin-protein isopeptide ligase activity.

172 ine phosphorylation of the ubiquitin-protein isopeptide ligase c-Cbl and promoted its interaction wit

173 function while binding the ubiquitin-protein isopeptide ligase Cbl.

174 the ElonginB/C-Cullin2-CIS ubiquitin-protein isopeptide ligase complex.

175 Ste6p* is dependent on the ubiquitin-protein isopeptide ligase Doa10p and is largely independent of t

176 The ubiquitin-protein isopeptide ligase E6-associated protein (E6AP) associate

177 Sina protein, function as ubiquitin-protein isopeptide ligase enzymes to target a wide range of cell

178 The Ras effector and ubiquitin-protein isopeptide ligase family member IMP acts as a steady-sta

179 e on MDM2 and inhibits its ubiquitin-protein isopeptide ligase function, resulting in the stabilizati

180 largely independent of the ubiquitin-protein isopeptide ligase Hrd1p/Der3p, whereas the opposite is t

181 gene, pVHL, functions as a ubiquitin-protein isopeptide ligase in regulating HIF-1 protein turnover,

182 PIASxbeta/Siz2, which is a ubiquitin-protein isopeptide ligase in the SUMO pathway, as the potential

183 pression of beta-TrCP1, an ubiquitin-protein isopeptide ligase interacting with ATF4 by RNA interfere

184 The ubiquitin-protein isopeptide ligase RNF8 was critical for 53BP1 focus targ

185 eport that the RING finger ubiquitin-protein isopeptide ligase Siah2 binds to and targets OGDHC-E2 fo

186 , a chaperone-dependent E3 ubiquitin-protein isopeptide ligase that is known to ubiquitylate other hs

187 in homologue Nrdp1/FLRF, a ubiquitin-protein isopeptide ligase that ubiquitinates ErbB3 and ErbB4, wa

188 Ps also possess E3 ligase (ubiquitin-protein isopeptide ligase) activities implicated in both caspase

189 ive form of the E3 ligase (ubiquitin-protein isopeptide ligase) that targets Runx2 for degradation (S

190 PMEPA1 protein is a NEDD4 (ubiquitin-protein isopeptide ligase)-binding protein, which negatively reg

191 As a ubiquitin-protein isopeptide ligase, cIAP1 ubiquitinated caspase-3 and -7,

192 hat inactivation of Cbl, a ubiquitin-protein isopeptide ligase, partially rescues T cell development

193 Nedd4-2, a ubiquitin-protein isopeptide ligase, tags ENaC with ubiquitin for internal

194 l ubiquitin exerts its effect at the step of isopeptide ligase-catalyzed (E3) ubiquitin conjugation s

195 culocyte extract demonstrated RAD6 supported isopeptide ligase-dependent degradation only through Lys

196 (PIAS) family members are ubiquitin-protein isopeptide ligase-small ubiquitin-like modifier ligases

197 n of Trim32, a RING domain ubiquitin-protein isopeptide ligase-ubiquitin ligase mutated in human limb

198 to both A3G and a Cullin 5 ubiquitin-protein isopeptide ligase.

199 Parkin is a ubiquitin-protein isopeptide ligase.

200 aining protein (beta-TrCP) ubiquitin-protein isopeptide ligase.

201 s on their catalytic context with respect to isopeptide ligase.

202 ts sumoylation, thus serving as SUMO-protein isopeptide ligases (E3) for SnoN sumoylation.

203 Siah proteins are ubiquitin-protein isopeptide ligases (E3) that have been implicated in a v

204 in, a motif often found in ubiquitin-protein isopeptide ligases (E3).

205 , which are believed to be ubiquitin-protein isopeptide ligases (type E3).

206 hat cIAP1 and cIAP2 are E3 ubiquitin-protein isopeptide ligases (ubiquitin ligases) for Smac.

207 tors for a large family of ubiquitin-protein isopeptide ligases to regulate certain signaling pathway

208 known as an adaptor in SCF ubiquitin-protein isopeptide ligases.

209 We provide the first demonstration that isopeptide ligation, a noncanonical activity of the enzy

210 s represent the first examples of successful isopeptide ligations starting from O-acyl Tyr-peptides.

211 ROC1 and APC11 immunocomplexes can catalyze isopeptide ligations to form polyubiquitin chains in an

212 x of these proteins stabilized by a covalent isopeptide linkage between Glu 112 and Lys beta400 of th

213 To determine the isopeptide linkage formed by BRCA1/BARD1-dependent polyu

214 but is instead dictated by how the substrate isopeptide linkage is oriented within the enzyme active

215 The isopeptide linkage of a polyubiquitin chain is a particu

216 Among seven possible isopeptide linkage sites in ubiquitin, K48 and K63 occur

217 s by human UCH-L3 of a 13-residue peptide in isopeptide linkage with ubiquitin, consistent with consi

218 ubiquitin chains containing a single type of isopeptide linkage, and that chains composed of linkages

219 to the SpyCatcher protein via a spontaneous isopeptide linkage, thereby offering a genetically encod

220 ss spectrometry (ESI-MS) method to determine isopeptide linkage-selectivity and affinity of poly-Ub.U

221 at 3.2 A resolution confirms the site of the isopeptide linkage.

222 t whose Lys-48 side chain is involved in the isopeptide linkage.

223 g that potentially not only peptide but also isopeptide linkages could be formed.

224 allow hOtu1 to discriminate among different isopeptide linkages in polyubiquitin substrates.

225 SpyCatcher, that spontaneously form covalent isopeptide linkages under physiological conditions.

226 -coil interactions or through intermolecular isopeptide linkages.

227 orks without affecting synthesis of standard isopeptide linkages.

228 x, which cleaves K11, K48, and K63 ubiquitin isopeptide linkages.

229 directly to lysine residues on Ubc9, forming isopeptide linkages.

230 ic ubiquitin-like protein) is deamidated and isopeptide linked to proteins by a mechanism distinct fr

231 uitins are added onto a substrate to form an isopeptide-linked 'polyubiquitin' chain, which targets s

232 ognizes K63-linked polyUb, but not any other isopeptide-linked (K6, K11, K27, K29, K33, or K48) polyU

233 tein fusion; UBICEP52) and representative of isopeptide-linked ubiquitin-protein conjugates [ubiquiti

234 BapA1 possesses nine putative pilin isopeptide linker domains which are crucial for pilus as

235 We show how site-specific isopeptide (Nepsilon-Gly-L-homothiaLys) bonds are forged

236 isolated isopeptides, but neither the major isopeptide nor the other minor isoform was found within

237 ases, enzymes that catalyze the formation of isopeptide protein-protein cross-links, are key enzymes

238 We confirmed that this isopeptide replacement is resistant to DUBs and to shavi

239 Exposure of these cells to NmU isopeptides resulted in an increase in intracellular Ca(

240 led equipotent, high affinity binding of NmU isopeptides to membranes prepared from D10.G4.1 cells.

241 echniques, hydrolysis of the non-fluorinated isopeptide was characterized by a burst phase followed b

242 The beta-lysyl-lysine isopeptide was identified in the exhaustive Pronase dige

243 eptides represented over 95% of the isolated isopeptides, which, at 2.5 nm concentration, induced the