ライフサイエンスコーパス: isotope labeling

1 of the aforementioned structures with (13)C-isotope labeling.

2 one-dimensional (1)H NMR spectra without any isotope labeling.

3 ned with experiments that incorporate stable isotope labeling.

4 liminating the need for either heteroatom or isotope labeling.

5 on or near the surface, without the need for isotope labeling.

6 quantification of the pattern and extent of isotope labeling.

7 nt of small molecules in plants using stable isotope labeling.

8 is probed with 2D IR spectroscopy and (13)C isotope labeling.

9 of LC-MS data, including in the presence of isotope labeling.

10 pids in monolayers and bilayers using stable isotope labeling.

11 icus rubellus were investigated using stable isotope labeling.

12 3-cyanobenzofurans with site specific (13)C-isotope labeling.

13 We have used stable isotope labeling ((15)N) of E. coli RNA in conjunction w

14 chlorophyll and proteins, a combined stable isotope labeling (15N)/mass spectrometry method was used

15 Through strategic isotope labeling, all nonhydrogen atoms were distinct fr

16 fatty acids and branched-chain amino acids), isotope labeling analyses supported the transformation o

17 icrobial mineralization using reverse stable isotope labeling analysis (RIL) of dissolved inorganic c

18 T, which was further confirmed by the stable isotope labeling analysis using deuterated acetate.

19 Subsequent stable isotope labeling analysis using deuterated ethanol deter

20 te whole-plant metabolic profiling by stable isotope labeling and combustion isotope-ratio mass spect

21 e one-carbon metabolic pathway, using stable-isotope labeling and detection of lysine methylation sig

22 ron diffraction in combination with hydrogen isotope labeling and empirical potential structure refin

23 Mechanistic studies including isotope labeling and Hammett correlation suggest that de

24 By combining selective isotope labeling and high-resolution solid-state Li NMR,

25 Here we used stable isotope labeling and isotopomer analysis to trace sterol

26 tion are typically conducted by using stable isotope labeling and label-free quantitation approaches.

27 of Met sulfoxide in proteins accurately, an isotope labeling and LC-MS peptide mapping method was de

28 ormational changes and dynamics using stable-isotope labeling and mass spectrometry (CDSiL-MS), which

29 Using metabolic stable isotope labeling and mass spectrometry, we demonstrate i

30 microbial nitrate-respiring communities with isotope labeling and metagenomics to unravel how specifi

31 The combination of stable isotope labeling and molecular network generation was sh

32 y combining microdialysis assays with simple isotope labeling and NMR experiments.

33 Whole-cell metabolic isotope labeling and quantitative MS analyses suggest th

34 d on whole cells and cell walls using stable-isotope labeling and rotational-echo double-resonance NM

35 e PDH-deficient parasites using rapid stable-isotope labeling and show that PDH does not appreciably

36 m of the present study was to combine stable isotope labeling and tandem mass spectrometry for the au

37 Using stable isotope labeling and tandem mass spectrometry, we quanti

38 Using site-specific isotope labeling and two-dimensional infrared (2D IR) sp

39 We have used isotope labeling and two-dimensional infrared spectrosco

40 k assignments were additionally supported by isotope-labeling and energy-resolved collision induced d

41 Recent breakthroughs in isotope-labeling and pulse sequence techniques have enab

42 Using a combination of MS, isotope labeling, and (1)H and (13)C NMR techniques, we

43 Kinetic studies, isotope labeling, and in situ high-resolution mass spect

44 f ovispirin using 2D IR line shape analysis, isotope labeling, and molecular dynamics simulations.

45 molecular homology modeling, in vivo stable isotope labeling, and transient expression in petunia fl

46 s monkeys in conjunction with in vivo stable-isotope-labeling, and dose-dependently reduced newly gen

47 A novel stable isotope labeling approach using (15)N and (18)O was appl

48 exchange a convenient and affordable stable isotope labeling approach.

49 quantification methods, specifically stable isotope labeling approaches such as isobaric tags and st

50 )C-MFA advanced methods for measuring stable-isotope labeling are needed.

51 p53 gene-derived DNA duplexes using a novel isotope labeling-based approach.

52 ibutions, are suitable for quantification of isotope-labeling-based studies, and provide additional i

53 oaches-microarray gene expression and stable isotope labeling by amino acids in cell culture (SILAC)

54 ate (ATP) affinity probe coupled with stable isotope labeling by amino acids in cell culture (SILAC)

55 Additionally, by comparing the stable isotope labeling by amino acids in cell culture (SILAC)

56 oal of the present study was to use a stable-isotope labeling by amino acids in cell culture (SILAC)

57 ication can be achieved by performing stable isotope labeling by amino acids in cell culture (SILAC)

58 Using stable isotope labeling by amino acids in cell culture (SILAC)

59 We combined stable isotope labeling by amino acids in cell culture (SILAC)

60 In this study, we adapted stable isotope labeling by amino acids in cell culture (SILAC)

61 Using Stable Isotope Labeling by Amino acids in Cell culture (SILAC)

62 quantified by using the differential stable isotope labeling by amino acids in cell culture (SILAC)

63 A two-state stable isotope labeling by amino acids in cell culture (SILAC)

64 hoproteomics that incorporates triple stable isotope labeling by amino acids in cell culture (SILAC)

65 ulture ((1)N/(1)N metabolic labeling, stable isotope labeling by amino acids in cell culture (SILAC))

66 A structure, we used a combination of stable isotope labeling by amino acids in cell culture (SILAC),

67 ation methods of biomolecules such as stable isotope labeling by amino acids in cell culture (SILAC),

68 We developed a stable isotope labeling by amino acids in cell culture (SILAC)-

69 Through comparative stable isotope labeling by amino acids in cell culture (SILAC)-

70 By using stable isotope labeling by amino acids in cell culture (SILAC)-

71 Super-stable isotope labeling by amino acids in cell culture (Super-S

72 Stable isotope labeling by amino acids in cell culture and quan

73 eomics analysis was carried out using stable isotope labeling by amino acids in cell culture combined

74 Stable isotope labeling by amino acids in cell culture experime

75 Stable isotope labeling by amino acids in cell culture followed

76 ed in vivo by mass spectrometry using stable isotope labeling by amino acids in cell culture mouse he

77 , we describe systematic quantitative stable isotope labeling by amino acids in cell culture proteomi

78 Quantitative proteomics based on stable isotope labeling by amino acids in cell culture showed t

79 We further performed stable isotope labeling by amino acids in cell culture to analy

80 clarify these issues, we used dynamic stable isotope labeling by amino acids in cell culture to defin

81 expression was revealed using pulsed stable isotope labeling by amino acids in cell culture to ident

82 We used a modified version of stable isotope labeling by amino acids in cell culture to ident

83 odifying the widely used technique of stable isotope labeling by amino acids in cell culture to inclu

84 Proteins, extracted from a SILAC (stable isotope labeling by amino acids in cell culture) labeled

85 SILAC (stable isotope labeling by amino acids in cell culture)-based m

86 ns, we conducted a family-wide SILAC (stable isotope labeling by amino acids in cell culture)-based p

87 etermined using a quantitative SILAC (stable isotope labeling by amino acids in cell culture)-based p

88 Stable isotope labeling by amino acids in cell culture, using p

89 s spectrometry-based technologies and stable isotope labeling by amino acids in cell culture, we ques

90 Using stable isotope labeling by amino acids in cell culture-based ma

91 exes and analyzed their components by stable isotope labeling by amino acids in cell culture-based ma

92 In a stable isotope labeling by amino acids in cell culture-based pr

93 In combination with the use of the stable isotope labeling by amino acids in cell culture-based qu

94 Furthermore, using stable isotope labeling by amino acids in culture (SILAC), we s

95 ic analyses of deletion strains using stable isotope labeling by amino acids in culture identified ot

96 iological light-dark conditions using stable isotope labeling by amino acids quantitative MS.

97 Stable isotope labeling by essential nutrients in cell culture

98 This methodology of stable isotope labeling by essential nutrients in cell culture

99 e life span estimates on the basis of stable isotope labeling can vary up to 10-fold among laboratori

100 etic beads, in conjunction with (18)O stable isotope labeling catalyzed by both trypsin and PNGaseF,

101 ing of the milk metabolome based on chemical isotope labeling (CIL) and liquid chromatography mass sp

102 thod was combined with differential chemical isotope labeling (CIL) LC-MS for mapping the metabolome

103 of filling the missing values in a chemical isotope labeling (CIL) LC-MS metabolomics platform.

104 We report a high-performance chemical isotope labeling (CIL) LC-MS method for profiling the ca

105 a metabolomic data set generated by chemical isotope labeling (CIL) liquid chromatography mass spectr

106 human sweat submetabolome based on chemical isotope labeling (CIL) liquid chromatography-mass spectr

107 High-performance chemical isotope labeling (CIL) liquid chromatography-mass spectr

108 a trap column for high-performance chemical isotope labeling (CIL) metabolomic profiling with deep c

109 a method based on high-performance chemical isotope labeling (CIL) nanoflow liquid chromatography ma

110 We used differential isotope labeling combined with mass spectrometric analys

111 The application of stable isotope labeling combined with SRM can overcome many of

112 gate this disparity, we generated new stable isotope labeling data in healthy adult subjects using bo

113 Dynamic isotope labeling data provides crucial information about

114 (13)C isotope labeling demonstrates that the reactive electrop

115 temperature dependence is perturbed by heavy isotope labeling, demonstrating a direct link between (p

116 An isotope labeling experiment is used to identify the oxyg

117 measured in vivo using a pulse-chase stable isotope labeling experiment.

118 Isotope labeling experiments and detailed DFT calculatio

119 nection between data obtained from elemental isotope labeling experiments and the well-known compartm

120 ynaMet for fully automated investigations of isotope labeling experiments from LC-high-resolution MS

121 Using genetic engineering and isotope labeling experiments in combination with infrare

122 he FFC algorithm is able to integrate stable isotope labeling experiments into the analysis and can a

123 trinsic reaction coordinate calculations and isotope labeling experiments of the reactions of D8-cycl

124 Isotope labeling experiments revealed a novel gamma-CH a

125 Stable isotope labeling experiments show that, after MeJA elici

126 Stable isotope labeling experiments showed that ADT1 suppressio

127 Isotope labeling experiments showed that the unsaturated

128 Through 13C-labeled isotope labeling experiments we elucidate that exosomes

129 Isotope labeling experiments with (18)O(2), (15)NO and N

130 Together with isotope labeling experiments, further support is provide

131 erent analytical platforms in the context of isotope labeling experiments.

132 Furthermore, isotope-labeling experiments demonstrate that the S-meth

133 Here, we describe the use of stable isotope-labeling experiments for studying metabolism und

134 Finally, isotope-labeling experiments rule out the alternative hy

135 A series of isotope-labeling experiments shed light on the bond reor

136 ns (MID) is of great significance for stable isotope-labeling experiments.

137 analyzing untargeted LC/MS data from stable isotope-labeling experiments.

138 ted and shown to resolve past mysteries from isotope-labeling experiments.

139 d database searching, which we enhance using isotope labeling for mixed NDE1-NDEL1.

140 lubility and ionization, and utilizes stable isotope labeling for MS1 level identification of hydroph

141 tein synthesis, optimized combinatorial dual-isotope labeling for nearly instant resonance assignment

142 ew glycomics method, termed glycan reductive isotope labeling (GRIL), where free glycans are derivati

143 Mass spectrometry-based stable isotope labeling has become a key technology for protein

144 ially useful in rapid carboxylic acid carbon isotope labeling, however development toward its applica

145 h the complementary application of oxygen-18 isotope-labeling, HPLC combined with electrospray ioniza

146 measure carbohydrate composition and stable-isotope labeling in algal biomass using gas chromatograp

147 s a photo-cross-linking strategy with stable isotope labeling in cell culture (SILAC)-based quantitat

148 y blue-native gel electrophoresis and stable isotope labeling in cell culture proteomics that the TbS

149 rotein mass spectrometry with dynamic stable isotope labeling in cell culture to achieve a proteome-w

150 AACT/SILAC (amino acid-coded tagging/stable isotope labeling in cell culture)-based quantitation met

151 eatly propelled by the development of stable isotope labeling in cell cultures (SILAC), a set of stan

152 ly, PAF-C purifications combined with stable isotope labeling in cells (SILAC) quantitation for PAF-C

153 We conclude that stable isotope labeling in healthy humans is consistent with a

154 describe use of quantitative in vivo stable isotope labeling in mammals to accurately compare serum

155 resolution orbital trap was used to quantify isotope labeling in peptides that were obtained from unl

156 f the protein dynamic was studied by protein isotope labeling in the framework of the Variational Tra

157 We made use of stable isotope labeling in tissue culture (SILAC) to identify I

158 h a range of gas-phase techniques, including isotope labeling, infrared photodissociation spectroscop

159 Isotope labeling is a powerful technique to probe detail

160 Our study shows that (15)N and (18)O isotope labeling is a useful approach for direct quantif

161 Stable isotope labeling is central to NMR studies of nucleic ac

162 sing sparse NOE data combined with selective isotope labeling is presented.

163 Stable isotope labeling is the state of the art technique for i

164 Isotope labeling is used for the study of TTR by NMR, ne

165 at this approach, which does not require any isotope labeling, is applicable to ligand-target systems

166 Assisted by the isotope labeling, it was possible to determine the seque

167 A stable isotope labeling kinetics experiment in NHPs was perform

168 the translation of the human in vivo stable-isotope-labeling kinetics (SILK) method to a rhesus monk

169 ells with (13)C methionine and measuring the isotope-labeling kinetics of both intracellular and extr

170 . coli strains using a differential chemical isotope labeling LC-MS platform.

171 We present a high-performance chemical isotope labeling liquid chromatography mass spectrometry

172 d (UMS) method, in conjunction with chemical isotope labeling liquid chromatography-mass spectrometry

173 c metabolite standards via the use of stable isotope labeling, liquid chromatography mass spectrometr

174 , global untargeted metabolomics, and stable isotope labeling mass spectrometry to identify metabolic

175 Stable-isotope-labeling mass spectrometry involves the addition

176 ral parameters for biomembrane systems where isotope labeling may be prohibitive.

177 To address this issue, a new stable isotope labeling method that targets tyrosine residues b

178 membranes, but it is challenging to use the isotope labeling method to study interfacial biomolecule

179 ddition to the Ile, Leu, Val (ILV) selective isotope labeling methodology adopted for NMR studies of

180 idues for modification by existing selective isotope labeling methods and use in relative quantitatio

181 Stable isotope-labeling methods, coupled with novel techniques

182 Stable isotope labeling-multiple reaction monitoring mass spect

183 Using stable isotope labeling of amino acids in a cell culture phosph

184 lmonary origin using the technique of stable isotope labeling of amino acids in cell culture (SILAC)

185 By coupling our methodology with stable-isotope labeling of amino acids in cell culture (SILAC),

186 hed fractions, which were compared by stable isotope labeling of amino acids in cell culture (SILAC)-

187 -inducible RTA expression and applied stable isotope labeling of amino acids in cell culture (SILAC)-

188 approaches such as isobaric tags and stable isotope labeling of amino acids in cell culture (SILAC).

189 nical amino acid tagging (BONCAT) and stable-isotope labeling of amino acids in cell culture (SILAC).

190 r SII stimulation using a strategy of stable isotope labeling of amino acids in cell culture (SILAC).

191 ce in combination with in vivo pulsed stable isotope labeling of amino acids in cell culture proteomi

192 We used the stable isotope labeling of amino acids in cell culture proteomi

193 were screened by quantitative SILAC (stable isotope labeling of amino acids in cell culture) co-immu

194 Using a SILAC (stable isotope labeling of amino acids in cell culture)-based p

195 ing)-based transcriptomics and SILAC (stable isotope labeling of amino acids in cell culture)-based q

196 Using stable isotope labeling of amino acids in culture (SILAC) and M

197 ll-wall architecture based on time-dependent isotope labeling of bacterial cells quantified by liquid

198 Stable isotope labeling of CNS proteins can be utilized to asse

199 w is straightforward, including differential isotope labeling of individual samples and a pooled samp

200 Surprisingly, results obtained with stable isotope labeling of mammals revealed that, in vivo, the

201 Isotope labeling of mESCs revealed that threonine provid

202 antifying metabolic fluxes based on tracking isotope labeling of metabolite within cells.

203 Differential (18)O/(16)O stable isotope labeling of peptides that relies on enzyme-catal

204 We report an enzymatic strategy for "stable isotope labeling of phosphonates in extract" (SILPE) tha

205 method is based on differential (12)C-/(13)C-isotope labeling of polyphenols through derivatization w

206 ntified by isotope shifts, but site-specific isotope labeling of proteins is today possible only for

207 The method was extended to the synthesis and isotope labeling of quinoline and 1,2,3,4-tetrahydroquin

208 re, this chemistry could be adapted to (13)C-isotope labeling of six pharmaceutically relevant compou

209 omatic ring, and it can be exploited for the isotope labeling of the aldehyde group.

210 By taking advantage of selective isotope labeling of the chains, we studied the role of i

211 Selective (15)N isotope labeling of the cytochrome bo(3) ubiquinol oxida

212 Analysis of constructs with selective isotope labeling of the delta1 methyl groups of isoleuci

213 etic information without the need for stable isotope labeling of the molecules of interest.

214 live cells using Raman microscopy with heavy isotope labeling of the peptide.

215 tive measurements can be performed by stable-isotope labeling of the peptides in the reductive dimeth

216 ts which unambiguously show PET and, through isotope labeling of the protein and the chromophore, are

217 es) has been achieved using stable (1)H/(2)D isotope labeling of the pyridyl donors and electrospray

218 Isotope labeling of the terminal oxo group with (18)O re

219 oped system was applied in the synthesis and isotope labeling of two pharmaceuticals, nordazepam and

220 A chemical isotope labeling or isotope coded derivatization (ICD) m

221 that minute spectral shifts induced by metal isotope labeling or temperature changes are detected usi

222 The isotope labeling patterns of 40 metabolites were obtaine

223 irect evidence for the Fl(N5[O]) species via isotope labeling, proteolytic digestion, and high-resolu

224 In this work an improved stable isotope labeling protocol for nucleic acids is introduce

225 We present a (13)C-based isotope labeling protocol for RNA.

226 e between bound and free ligand or on stable isotope labeling, relying instead on a tert-butyl group

227 fication protocol allowed for cost-effective isotope labeling required for a detailed NMR structural

228 Stable-isotope labeling reveals that even severely aggregated e

229 es, along with a judiciously designed stable isotope labeling scheme, to measure atomistic-resolution

230 agnetic resonance combined with an efficient isotope labeling scheme.

231 ve to the chemical, metabolic, and enzymatic isotope-labeling schemes currently used in quantitative

232 We describe specific isotope-labeling schemes required for working with large

233 onstrates the feasibility and power of using isotope labeling SFG to probe molecular structures of in

234 Peptide quantitation based on stable isotope labeling showed that the surfactant induced 1.5-

235 odeled by the parasite cytoplasm, and stable isotope labeling shows some apicoplast lipids are genera

236 Stable isotope labeling (SIL) techniques have the potential to

237 The use of stable isotope labeling strategies that are adaptable to proteo

238 An isotope labeling strategy is described for efficient ide

239 As an isotope-labeling strategy for NMR studies, reductive met

240 Isotope labeling studies confirmed that the oxygen atom

241 Isotope labeling studies confirmed the origin of the met

242 We employ a series of kinetic and isotope labeling studies made largely possible by electr

243 Isotope labeling studies rule out a direct four-electron

244 Isotope labeling studies show that a hydrogen is transfe

245 Isotope labeling studies show that O(2) is the sole sour

246 Preliminary kinetics and isotope labeling studies suggest epoxide ring opening as

247 This dogma was recently challenged by stable isotope labeling studies with heavy water, which yielded

248 Isotope labeling studies, coupled with FTIR and CSI-MS,

249 Isotope-labeling studies suggest a mechanism proceeding

250 h the in situ spectroscopic measurements and isotope-labeling studies, support this mode of operation

251 An isotope-labeling study supports an oxidative cross-coupl

252 Degradation-reconstruction approaches for isotope labeling synthesis have been known for their rem

253 ein isoform analysis method utilizing stable isotope labeling tandem mass spectrometry (SILT MS).

254 Here, we used a stable isotope labeling technique ((18)O and (2)H) to determine

255 We also employed a stable-isotope labeling technique to illuminate high-priority m

256 Here, we applied a stable isotope-labeling technique in combination with mass spec

257 Stable isotope labeling techniques for quantitative top-down pr

258 Like popular bottom-up isotope labeling techniques, most top-down labeling meth

259 were analyzed by ESI/qTOF/MS using MS/MS and isotope labeling techniques.

260 roughs during the past decade, especially in isotope-labeling techniques, have enabled NMR characteri

261 Finally, we show using stable isotope labeling that in an embryonic mouse cell line, g

262 currently tenable due to the requirements of isotope labeling, the large size of the proteins, and th

263 en made thanks to the introduction of stable isotope labeling, the state-of-the-art technique for in

264 With the help of site-specific isotope labeling, the topologies of these two structures

265 t relies on phosphatase treatment and stable-isotope labeling to determine absolute stoichiometries o

266 We use 2D infrared spectroscopy and isotope labeling to monitor the kinetics of fibril forma

267 The approach was enhanced with stable isotope labeling to overcome ambiguities in determining

268 We further used stable-isotope labeling to trace the metabolic dynamics of fatt

269 rometry with metabolic [(2)H3]-leucine heavy isotope labeling under divergent conditions.

270 gh sensitivity, we developed cysteine-stable isotope labeling using amino acids in cell culture (SILA

271 ribe an integrated approach combining stable isotope labeling, various protein enrichment and extract

272 The (13)C-isotope labeling was achieved applying a Pd-catalyzed me

273 Using a metabolomics approach with isotope labeling, we found that in some cancer cells a r

274 mensional infrared spectra and site-specific isotope labeling, we have measured the development of se

275 c solvent, and gradient slope) and different isotope labelings were addressed by multiple-factor scre

276 include unpredictable mass shifts following isotope labeling, which impedes analysis of unknown prot

277 ethodology is also highly suitable for (13)C isotope labeling, which was demonstrated through the syn

278 We utilized stable isotope labeling with amino acids (SILAC) in PTECs to co

279 affinity purification), coupled with stable isotope labeling with amino acids in cell culture (SILAC

280 irus (IBV) N protein was mapped using stable isotope labeling with amino acids in cell culture (SILAC

281 technique, NeuCode (neutron encoding) stable isotope labeling with amino acids in cell culture (SILAC

282 ss spectrometry and quantification by Stable Isotope Labeling with Amino Acids in Cell Culture (SILAC

283 ic acid (17-ODYA) in combination with stable-isotope labeling with amino acids in cell culture (SILAC

284 We adapted stable-isotope labeling with amino acids in cell culture (SILAC

285 sine profiling method with 'spike-in' stable isotope labeling with amino acids in cell culture (SILAC

286 ent and non-adherent conditions using stable isotope labeling with amino acids in cell culture (SILAC

287 We coupled cell fractionation with stable isotope labeling with amino acids in cell culture (SILAC

288 Using stable isotope labeling with amino acids in cell culture and ma

289 Using stable isotope labeling with amino acids in cell culture and re

290 us proteins in a given sample (e.g., stabile isotope labeling with amino acids in cell culture is not

291 this study was generated using SILAC (Stable Isotope Labeling with Amino acids in Cell culture) techn

292 's composition in samples prepared by stable isotope labeling with amino acids in cell culture, using

293 sis of the TIM23 interactome based on stable isotope labeling with amino acids in cell culture.

294 Using stable isotope labeling with amino acids in culture (SILAC) cou

295 ity-labeled proteins were analyzed by stable isotope labeling with amino acids in culture (SILAC)-LC/

296 In this paper, we describe a stable isotope labeling with amino acids in culture-based quant

297 Stable isotope labeling with multiple reaction monitoring-mass

298 Our approach in combining stable isotope labeling with NanoSIMS and TEM imaging can be ex

299 relative quantification via enzyme-mediated isotope labeling with this signature digestion approach

300 quency spectroscopic approaches or selective isotope labeling would be desirable for unambiguous assi