ライフサイエンスコーパス: jannaschii

1 rthermophilic methanogen, Methanocaldococcus jannaschii.

2 S2P metalloprotease from Methanocaldococcus jannaschii.

3 and syntrophic coculture with Methanococcus jannaschii.

4 mino acid biosynthesis in Methanocaldococcus jannaschii.

5 Clostridium difficile and Methanocaldococcus jannaschii.

6 ponsible for generating 6-deoxyhexoses in M. jannaschii.

7 mologous E/F complex from Methanocaldococcus jannaschii.

8 ed and characterized from Methanocaldococcus jannaschii.

9 NA polymerase (RNAP) from Methanocaldococcus jannaschii.

10 a subunits from the methanogen Methanococcus jannaschii.

11 pathway leading to DHQ in Methanocaldococcus jannaschii.

12 ducts catalyze Cys-tRNA(Cys) synthesis in M. jannaschii.

13 extracts of the archaeon Methanocaldococcus jannaschii.

14 nt with the higher temperature habitat of M. jannaschii.

15 lved in the synthesis of Cys-tRNA(Cys) in M. jannaschii.

16 ures that are physiologically relevant to M. jannaschii.

17 DHNA from the methanogen Methanocaldococcus jannaschii.

18 Methanosarcina thermophila and Methanococcus jannaschii.

19 thermoautotrophicum, M. thermophila, and Mc. jannaschii.

20 hyperthermophilic euryarchaeon Methanococcus jannaschii.

21 case from the hyperthermophile Methanococcus jannaschii.

22 oserine phosphatase (PSP) from Methanococcus jannaschii.

23 ized from the hyperthermophile Methanococcus jannaschii.

24 hii; and (iii) the use of proteomics with M. jannaschii.

25 ource of beta-alanine in cell extracts of M. jannaschii.

26 aeoglobus fulgidus, and Methanocaldocococcus jannaschii.

27 egulatory protein, GlnK1, from Methanococcus jannaschii.

28 the thermophilic archaeon Methanocaldococcus jannaschii.

29 romoters in the genome of Methanocaldococcus jannaschii.

30 the fibrillarin homologue from Methanococcus jannaschii, a hyperthermophile, at 1.6 A resolution.

31 The methanarchaeon, Methanococcus jannaschii, a hyperthermophilic, autotrophic, and strict

32 t organisms except for some Archaea (e.g. M. jannaschii, A. fulgidus) and some pathogens (e.g. Helico

33 studied the Trx system of Methanocaldococcus jannaschii--a deeply rooted hyperthermophilic methanogen

34 ve the transcription of two copies of the M. jannaschii aaRS gene.

35 M. jannaschii AdoMetDC has a Km of 95 microm and the turnov

36 s that alter the metal specificity of the M. jannaschii agmatinase from Mn(II) to Fe(II).

37 richia coli using evolved Methanocaldococcus jannaschii aminoacyl-tRNA synthetase(s) (aaRS)/suppresso

38 Therefore, Fsr provides M. jannaschii an anabolic ability and protection from sulfi

39 MJ1541 gene product from Methanocaldococcus jannaschii, an enzyme that was annotated as a 5'-methylt

40 In contrast, the IMPase from Methanococcus jannaschii, an organism in which DIP does not accumulate

41 Escherichia coli, archaea like Methanococcus jannaschii and animal viruses.

42 hereas ThrRS enzymes from Methanocaldococcus jannaschii and Archaeoglobus fulgidus were not.

43 er I-1-Pases (from the archaea Methanococcus jannaschii and Archaeoglobus fulgidus, and from the bact

44 different cysteinyl-tRNA synthetase from M. jannaschii and Deinococcus radiodurans and its character

45 gonal aaRSes derived from Methanocaldococcus jannaschii and Escherichia coli tyrosyl-tRNA synthetases

46 terium thermoautotrophicum and Methanococcus jannaschii and in Bacillus subtilis.

47 the genomes of Archae such as Methanococcus jannaschii and Methanobacterium thermoautotrophicum has

48 he transcription factor IIB of Methanococcus jannaschii and Methanocaldococcus vulcanius, revealing a

49 ophicus, and hyperthermophiles Methanococcus jannaschii and Methanococcus igneus were studied to dete

50 also confirmed by using cell extracts of M. jannaschii and Methanosarcina thermophila.

51 The methanogenic archaea Methanocaldococcus jannaschii and Methanothermobacter thermautotrophicus co

52 that mimicked the RPAs in Methanocaldococcus jannaschii and Methanothermobacter thermautotrophicus th

53 enzymes from the archaea Methanocaldococcus jannaschii and Methanothermobacter thermautotrophicus, f

54 , its operon organization with Methanococcus jannaschii and Methanothermobacter thermoautotrophicum.

55 homologues were found in Methanocaldococcus jannaschii and Methanothermobacter thermoautotrophicus,

56 genes, MJ1425 and MJ0490, from Methanococcus jannaschii and one from Methanothermus fervidus were clo

57 hown that Methanocaldococcus (Methanococcus) jannaschii and other anaerobic archaea synthesize cataly

58 hospho-D-ribose-1-pyrophosphate (PRPP) in M. jannaschii and other methanogenic archaea.

59 aeolicus,47 Bacillus subtilis, Methanococcus jannaschii and Pseudomonas aeruginosa that provide stron

60 GC-rich regions in the AT-rich Methanococcus jannaschii and Pyrococcus furiosus genomes efficiently d

61 erthermophilic marine archaeon Methanococcus jannaschii and shown to catalyze the final reactions in

62 haea, including the methanogen Methanococcus jannaschii and the sulfate-reducing archaeon Archaeoglob

63 s of the F and P subunits from Methanococcus jannaschii and used them in in vitro and in vivo protein

64 ethanococcus maripaludis, Methanocaldococcus jannaschii, and Sulfolobus solfataricus.

65 (ii) experimental functional genomics for M. jannaschii; and (iii) the use of proteomics with M. jann

66 omes and whole-cell lysates of Methanococcus jannaschii (approximately 1,800 genes) using a 2D protei

67 ombe yeast, the E. coli bacterium and the M. jannaschii archaebacterium.

68 ort, it is shown not only that Methanococcus jannaschii, Archaeoglobus fulgidus, Methanosarcina aceti

69 , have been identified in Methanocaldococcus jannaschii as the enzymes involved in the synthesis of D

70 the methanogenic archaeon Methanocaldococcus jannaschii as well as most methanogens, none of the expe

71 of a specificity subunit from Methanococcus jannaschii at 2.4-A resolution.

72 structure of the complex from Methanococcus jannaschii at a resolution of 3.2 A.

73 the sequenced genomes of Methanocaldococcus jannaschii, Bacillus cereus ATCC 10987 and Methylococcus

74 We propose that ORF MJ1117 of M. jannaschii be annotated as cobY to reflect its involveme

75 EM to elucidate the sRNA orientation in a M. jannaschii box C/D di-sRNP.

76 and circular forms) using Methanocaldococcus jannaschii box C/D RNP core proteins.

77 /D sRNP from the archaeon Methanocaldococcus jannaschii by single-particle electron microscopy.

78 the related MjNhaP1 from Methanocaldococcus jannaschii can be attributed to an additional negatively

79 the tyrosyl-tRNA synthetase of Methanococcus jannaschii can be used to genetically encode unnatural a

80 chaeal Sulfolobus shibatae and Methanococcus jannaschii CCA-adding enzymes, are also capable of poly(

81 tography-mass spectrometry analysis of an M. jannaschii cell extract showed the presence of free form

82 al and NADH, NADPH, F 420H 2, or DTT to a M. jannaschii cell extract stimulated the production of bot

83 y incorporating 13C into the formate when M. jannaschii cell extracts were incubated with H13CO3- and

84 ed into dehydroshikimate and shikimate in M. jannaschii cell extracts, consistent with the remaining

85 in reactions catalyzed by A. fulgidus and M. jannaschii cell extracts.

86 n of the aspartate transcarbamoylase from M. jannaschii cell-free extract revealed that the enzyme ha

87 rmophiles Aquifex aeolicus and Methanococcus jannaschii complement enteric amtB mutants for growth at

88 of FAICAR synthetase from Methanocaldococcus jannaschii complexed with various ligands, including the

89 e hyperthermophilic methanogen Methanococcus jannaschii contains homologs of most genes required for

90 y class V aspartate aminotransferase from M. jannaschii converted the phosphohydroxypyruvate product

91 Kinetic studies show that M. jannaschii DHNA possesses a catalytic capability with a

92 totypical NBD MJ0796 from Methanocaldococcus jannaschii dimerizes in response to ATP binding and diss

93 at of non-nitrogen fixing Methanocaldococcus jannaschii DSM 2661.

94 esponding fragment of the Methanocaldococcus jannaschii Elp3 protein.

95 ic, methanogenic archaeon Methanocaldococcus jannaschii encodes a CobY protein (Mj CobY) that transfe

96 that the MJ0438 gene from Methanocaldococcus jannaschii encodes a novel S-adenosylmethionine-dependen

97 yperthermophilic archaeon Methanocaldococcus jannaschii encodes a potent transcriptional activator, P

98 The predicted ORF MJ1140 in the genome of M. jannaschii encodes ComB, a Mg2+-dependent acid phosphata

99 The genome sequence of M. jannaschii encodes two homologs of each large and small

100 The hyperthermophilic archaeon Methanococcus jannaschii encodes two putative transcription regulators

101 The hyperthermophilic archaeon Methanococcus jannaschii encodes two putative transcription regulators

102 the C-terminal domain of Methanocaldococcus jannaschii endonuclease.

103 Here we report that the Methanocaldococcus jannaschii enzyme derived from the MJ0309 gene is an Fe(

104 The bifunctional activity of this M. jannaschii enzyme illustrates the evolution of a suprafa

105 me places a stronger emphasis on G35, the M. jannaschii enzyme places a stronger emphasis on G36, a f

106 (67), and Cys(272) in the Methanocaldococcus jannaschii enzyme) are essential for the sulfhydrylation

107 an end product of the purified recombinant M.jannaschii enzyme.

108 he overall structural conservation of the M. jannaschii F subunit, although not readily recognizable

109 , as well as direct enzymatic assays with M. jannaschii, failed to reveal the presence of PRK.

110 teins from the methanogen Methanocaldococcus jannaschii formed the first homoaconitase shown to catal

111 The Methanococcus jannaschii gene MJ0671 was cloned and overexpressed in E

112 Methanocaldococcus jannaschii gene MJ1179 encodes a protein that is classif

113 +C)% screening alone, a 1% fraction of the M.jannaschii genome containing all 44 known transfer RNAs,

114 d archaeal protein in the Methanocaldococcus jannaschii genome, MJ0887, which could be involved in th

115 SAM) enzymes account for nearly 2% of the M. jannaschii genome, where the major SAM derived products

116 r anticipated pathway could produce DKFP, M. jannaschii glucose-6-P metabolism was studied in detail

117 Yet, we observed that M. jannaschii grows and produces methane with sulfite as th

118 enzymes, the hyperthermophile Methanococcus jannaschii has an enzyme, DCD-DUT, that harbors both dCT

119 yde dehydrogenase (ASADH) from Methanococcus jannaschii has been determined to 2.3 angstroms resoluti

120 The riboflavin kinase in Methanocaldococcus jannaschii has been identified as the product of the MJ0

121 hyperthermophilic euryarchaeon Methanococcus jannaschii has no recognizable homologues of the canonic

122 The hyperthermophilic archaeon Methanococcus jannaschii has two members of this gene family.

123 yde and hydroxyacetone in Methanocaldococcus jannaschii have been established.

124 Cell extracts of Methanococcus jannaschii have been shown to readily convert L-ornithin

125 yses of the F(420)s present in Methanococcus jannaschii have shown that these cells contain a series

126 se encoded by the ComA gene in Methanococcus jannaschii have suggested that ComA, which catalyzes the

127 Using the M. jannaschii high-temperature in vitro transcription syste

128 combination of the homoaconitase with the M. jannaschii homoisocitrate dehydrogenase catalyzed all of

129 The M. jannaschii homolog of XecG, MJ0255, is located next to a

130 syn conformation reported for Methanococcus jannaschii homoserine kinase.

131 high-resolution structures of Methanococcus jannaschii HSK ternary complexes with its amino acid sub

132 nduced the ordered oligomer of Methanococcus jannaschii Hsp16.5 to transition to either expanded symm

133 an engineered variant of Methanocaldococcus jannaschii Hsp16.5 wherein a 14 amino acid peptide from

134 from that of zinc-loaded Methanocaldococcus jannaschii HypB as well as subtle changes to the protein

135 of the A. fulgidus enzyme and not in the M. jannaschii IMPase, the disruption (e.g., A. fulgidus IMP

136 he structure of KsgA from Methanocaldococcus jannaschii in complex with several ligands, including th

137 from the archaebacterium Methanocaldococcus jannaschii in its free form (2.2 A resolution) and bound

138 ere observed in (G+C)% and, in Methanococcus jannaschii, in the frequency of the dinucleotide 'CG'.

139 with the related organism Methanocaldococcus jannaschii included the absence of inteins, even though

140 rresponding activity from Methanocaldococcus jannaschii indicated that tRNA(Cys) becomes acylated wit

141 known structure, MJ0796, from Methanococcus jannaschii indicates that at least two binding sites par

142 Methanocaldococcus jannaschii is a hypertheromphilic, strictly hydrogenotro

143 AdoMetDC from the Archaea Methanococcus jannaschii is a prototype for a recently discovered clas

144 rtate decarboxylase (PanD), the enzyme in M. jannaschii is a pyridoxal phosphate (PLP)-dependent l-as

145 lts indicate that proline biosynthesis in M. jannaschii is accomplished by a previously unrecognized

146 sals that aminoacylation with cysteine in M. jannaschii is an auxiliary function of a canonical proly

147 The enzyme from Methanocaldococcus jannaschii is designated MptA to indicate that it cataly

148 19 gene in the methanogen Methanocaldococcus jannaschii is likely this missing methylase.

149 inary studies had shown that L-lactate in M. jannaschii is not derived from pyruvate, and thus an alt

150 us putative gene (MJ1604) from Methanococcus jannaschii is now annotated as a phosphofructokinase, wh

151 Whereas ProRS from Methanococcus jannaschii is similar to E. coli in its ability to hydro

152 zyme encoded by Mj0883 of Methanocaldococcus jannaschii is the archaeal counterpart of the bacterial

153 the hyperthermophilic Archaea Methanococcus jannaschii, is a member of the small heat-shock protein

154 th isoprene biosynthesis, Methanocaldococcus jannaschii isopentenyl phosphate kinase is predicted to

155 iae, Escherichia coli and Methanocaldococcus jannaschii It presents the following data: (i) hierarchi

156 s demonstrate that recombinant Methanoccocus jannaschii L7 protein binds the box C/D snoRNA core moti

157 The crystal structure of Methanocaldococcus jannaschii L7Ae has been determined to 1.45 A, and L7Ae'

158 Notably, the free M. jannaschii L7Ae structure is essentially identical to th

159 ir highest Blastp hits in Methanocaldococcus jannaschii, lateral gene transfer or gene loss has appar

160 talytic residue from a related Methanococcus jannaschii LonB, also faces the solvent and does not int

161 In the archaebacterium Methanocaldococcus jannaschii (M. jannaschii), the proteasomal regulatory p

162 Furthermore, recombinant M. jannaschii, M. acetivorans, and A. fulgidus RubisCO poss

163 and efficient comparison of histones from M. jannaschii, Methanosarcina acetivorans (largest Archaeal

164 ic and non-halophilic Archaea (Methanococcus jannaschii, Methanosarcina mazei, Methanobrevibacter smi

165 However, the genomes of Methanocaldococcus jannaschii, Methanothermobacter thermautotrophicus, and

166 ve not been identified in Methanocaldococcus jannaschii, Methanothermobacter thermautotrophicus, or M

167 heat-shock protein (sHSP) from Methanococcus jannaschii (Mj HSP16.5) forms a homomeric complex of 24

168 e complex of the archaeal Methanocaldococcus jannaschii (Mj) Ago.

169 The interaction of Methanocaldococcus jannaschii (Mj) Nop56/58 with the methyltransferase fibr

170 opentenyl kinase (IPK) in Methanocaldococcus jannaschii (MJ) suggests a new variation of the MVA path

171 RNA splicing endonuclease from Methanococcus jannaschii (MJ), indicating its role in catalysis.

172 tuted-nucleotides by FtsZ from Methanococcus jannaschii (Mj-FtsZ) and Bacillus subtilis (Bs-FtsZ).

173 reaction analogous to that of Methanococcus jannaschii MJ0044).

174 previously solved enzyme from Methanococcus jannaschii (MJ0109).

175 The Methanocaldococcus jannaschii MJ0116 gene was cloned and heterologously exp

176 the corresponding protein from Methanococcus jannaschii, MJ0158.

177 t-stable DmrX analog from Methanocaldococcus jannaschii (MJ0208).

178 The protein product of the M. jannaschii MJ0400 gene catalyzes the transaldolase react

179 ve been proposed: one based on Methanococcus jannaschii MJ0577 (1MJH) that binds ATP, and the other b

180 ersion of PRPP to RuBP were identified in M. jannaschii (Mj0601) and Methanosarcina acetivorans (Ma28

181 The protein product of the Methanococcus jannaschii MJ0768 gene has been expressed in Escherichia

182 ed a prototypical ABC NBD, the Methanococcus jannaschii MJ0796, using spectroscopic techniques.

183 by Bacillus subtilis YqeV and Methanococcus jannaschii Mj0867, and we predict that RimO is unique am

184 Methanococcus jannaschii MJ0936 is a hypothetical protein of unknown f

185 e corresponding gene from Methanocaldococcus jannaschii (mj1022) and characterized the purified recom

186 d that the protein was the product of the M. jannaschii MJ1025 gene.

187 The Methanococcus jannaschii MJ109 gene product, the sequence of which is

188 The protein product of the Methanococcus jannaschii MJ1256 gene has been expressed in Escherichia

189 oding the DapL homolog in Methanocaldococcus jannaschii (MJ1391) was cloned and expressed in Escheric

190 jannaschii prolyl-tRNA synthetase or the M. jannaschii MJ1477 protein provides the "missing" CysRS a

191 Using four of the Methanocaldococcus jannaschii (Mja) histones, we have examined activation o

192 Methanocaldococcus jannaschii (Mja) Ptr2, a homologue of the Lrp/AsnC famil

193 conjugate [pre-tRNA(Tyr)-Methanocaldococcus jannaschii (Mja) RPR] to investigate the functional role

194 the C-terminal stirrup of Methanocaldococcus jannaschii (Mja) TBP do not completely abrogate basal tr

195 as that from the archaeon Methanocaldococcus jannaschii (Mja), to catalyze precursor tRNA (ptRNA) pro

196 rom the archaeal organism Methanocaldococcus jannaschii (MjAgo) possesses two modes of action: the ca

197 enzyme from the archaeon Methanocaldococcus jannaschii (MjCobY) in complex with GTP is reported.

198 domain-containing channel from Methanococcus jannaschii, MjK2, by testing its general functional beha

199 the hyperthermophilic archaeon Methanococcus jannaschii (MjMAT) is a prototype of the newly discovere

200 (+) exchanger, NhaP1 from Methanocaldococcus jannaschii (MjNhaP1), a close homologue of the medically

201 Here,Methanococcus jannaschii MR-ATPgammaS-DNA structure reveals that the p

202 del based on the structures of Methanococcus jannaschii Mre11 (MjMre11) bound to longer DNA molecules

203 The archaeal NCX_Mj (Methanococcus jannaschii NCX) system was used to resolve the backbone

204 change function of an NCX from Methanococcus jannaschii (NCX_Mj) and report its 1.9 angstrom crystal

205 We then tested a hypothetical M. jannaschii O-phosphoseryl-tRNA(Sec) kinase and demonstra

206 of this transformation in Methanocaldococcus jannaschii occurs by the reaction of 4-hydroxybenzoic ac

207 otted protein MJ0366 from Methanocaldococcus jannaschii on the operation of the ClpXP protease from E

208 el complexes derived from Methanocaldococcus jannaschii or Escherichia coli show the channel in its c

209 TAG, together with orthogonal Methanococcus jannaschii or Escherichia coli tRNA/synthetase pairs hav

210 Methanococcus jannaschii partially retains the superoperon, but lacks

211 f a multidomain protein, as in Methanococcus jannaschii peptidyl-prolyl cis/trans isomerase FKBP26.

212 otein translation apparatus of Methanococcus jannaschii possesses the unusual enzyme prolyl-cysteinyl

213 m5 enzyme of the archaeon Methanocaldococcus jannaschii (previously MJ0883) as an example, we have cr

214 anococcus maripaludis and Methanocaldococcus jannaschii produce cysteine for protein synthesis using

215 us abyssi proabylysin and Methanocaldococcus jannaschii projannalysin), which are soluble and, with a

216 Methanocaldococcus jannaschii prolyl-tRNA synthetase (ProRS) was previously

217 It was reported that M. jannaschii prolyl-tRNA synthetase or the M. jannaschii M

218 se results on the in vivo activity of the M. jannaschii ProRS and on the nature of the enzyme involve

219 Although M. jannaschii ProRS catalyzes the synthesis of Cys-tRNA(Pro

220 The archaeal Methanococcus jannaschii ProRS is a member of the eukaryotic-like grou

221 cent biochemical experiments showing that M. jannaschii ProRS misacylates tRNA(Pro) with cysteine, an

222 pure heterologously expressed recombinant M. jannaschii ProRS misaminoacylates M. jannaschii tRNA(Pro

223 ome respects, recognition of tRNA(Pro) by M. jannaschii ProRS parallels that of human, with a strong

224 at resolutions between 2.6 and 3.2 A: apo M. jannaschii ProRS, and M. thermautotrophicus ProRS in apo

225 Studies with human and Methanococcus jannaschii ProRS, which lack a post-transfer editing dom

226 ovide evidence of divergent adaptation by M. jannaschii ProRS; recognition of the tRNA acceptor end i

227 tion NMR study of a model ABC, Methanococcus jannaschii protein MJ1267, reveals that ADP-Mg binding a

228 rted the structure of the Methanocaldococcus jannaschii PSTK (MjPSTK) complexed with AMPPNP.

229 A(Sec) to investigate how Methanocaldococcus jannaschii PSTK distinguishes tRNA(Sec) from tRNA(Ser).

230 mical characterization of Methanocaldococcus jannaschii PSTK, including kinetics of Sep-tRNA(Sec) for

231 ontrast, the effector domain of Ptr1, the M. jannaschii Ptr2 paralogue, yields only very weak activat

232 Methanocaldococcus jannaschii Ptr2, a member of the Lrp/AsnC family of bact

233 rP, is the product of the Methanocaldococcus jannaschii purP gene (MJ0136), which is a signature gene

234 riosus PurP is structurally homologous to M. jannaschii PurP.

235 gel patterns for Homo sapiens, Methanococcus jannaschii, Pyro coccus furiosus, Shewanella oneidensis,

236 sis of coenzyme F(420) in Methanocaldococcus jannaschii requires generation of 2-phospho-L-lactate, w

237 philes Thermotoga maritima and Methanococcus jannaschii resulted in fivefold higher T. maritima cell

238 rystal structure of the SecY channel from M. jannaschii revealed a plug domain that appears to seal t

239 loped a fluorescently labeled recombinant M. jannaschii RNAP system to probe the archaeal transcripti

240 By studying the archaeal Methanocaldococcus jannaschii RPR's cis cleavage of precursor tRNA(Gln) (pr

241 vir and its analogs inhibit human homolog M. jannaschii S2P cleavage of an artificial protein substra

242 solved X-ray structure of the Methanococcus jannaschii SecY complex, is a matter of contention.

243 d affinity for E. coli seryl-tRNA(Sec) or M. jannaschii seryl-tRNA(Sec), and neither substrate was di

244 s of equivalent plug deletions in SecY of M. jannaschii show that, although the overall structures ar

245 jR31K mutant of PurO from Methanocaldococcus jannaschii showed 76% decreased activity and the MjE102Q

246 us strains from Axial and Methanocaldococcus jannaschii showed similar Monod growth kinetics when gro

247 antiporter NCX_Mj protein from Methanococcus jannaschii shows an outward-facing conformation suggesti

248 ve solved the structure of the Methanococcus jannaschii Spt4/5 complex by X-ray crystallography, and

249 A second dual-guide box C/D sRNA from M. jannaschii, sR6, also exhibited RNA remodeling during te

250 rarchical assembly of the Methanocaldococcus jannaschii sR8 box C/D sRNP is a temperature-dependent p

251 Surprisingly, M.jannaschii SRP RNA bound to human SRP54M quantitatively

252 r ability to bind to human and Methanococcus jannaschii SRP RNA were determined in vitro.

253 e mechanism of Cys-tRNA(Cys) formation in M. jannaschii still remains to be discovered.

254 hich is different from that of Methanococcus jannaschii, strongly supports an active role in the cata

255 ncy screening was used, 43 of the 44 known M.jannaschii structural ncRNAs were again identified, whil

256 ns, Methanopyrus kandleri, and Methanococcus jannaschii, suggesting a conservation of its function ac

257 phosphate pyrophosphatase from Methanococcus jannaschii that shows a preference for purine base analo

258 protein from the archaeon Methanocaldococcus jannaschii that was copurified with prolyl-tRNA syntheta

259 ebacterium Methanocaldococcus jannaschii (M. jannaschii), the proteasomal regulatory particle (RP), a

260 In the archaea Methanocaldococcus jannaschii, the RP is a homohexameric complex of proteas

261 ide of 645 amino acids, as for Methanococcus jannaschii, the Sulfolobus solfataricus SSB protein (Sso

262 yclodeaminase is present in the genome of M. jannaschii, these results indicate that proline biosynth

263 ial genomes ranged from 8% for Methanococcus jannaschii to 37% for Mycoplasma pneumoniae.

264 ome-wide occupancy of the Methanocaldococcus jannaschii transcription machinery and its transcriptome

265 CAU and CAC, by an engineered orthogonal M. jannaschii tRNA with an AUG anticodon: tRNA(Opt) We susp

266 the identity elements of Methanocaldococcus jannaschii tRNA(Cys) in the aminoacylation reaction for

267 is unable to aminoacylate purified mature M. jannaschii tRNA(Cys) with cysteine in contrast to facile

268 ow here that the unmodified transcript of M. jannaschii tRNA(Pro) is indeed mis-acylated with cystein

269 nant M. jannaschii ProRS misaminoacylates M. jannaschii tRNA(Pro) with cysteine.

270 For Methanococcus jannaschii tRNA(Pro), accuracy is difficult because the

271 We introduced a Methanocaldococcus jannaschii tRNA:aminoacyl-tRNA synthetase pair into the

272 uctures of two substrate-bound Methanococcus jannaschii tyrosyl aminoacyl-tRNA synthetases that charg

273 and the genetic code, only the Methanococcus jannaschii tyrosyl tRNA synthetase and tRNA have been us

274 The Methanococcus jannaschii tyrosyl-transfer-RNA synthetase-tRNA(CUA) (Mj

275 essor tRNA derived from a Methanocaldococcus jannaschii tyrosyl-tRNA (MjtRNATyrCUA) are expressed und

276 ply it to the design of mutant Methanococcus jannaschii tyrosyl-tRNA synthetase (M.jann-TyrRS).

277 r, and a gene encoding the desired mutant M. jannaschii tyrosyl-tRNA synthetase (MjTyrRS) is expresse

278 We synthesized 1 and evolved a Methanococcus jannaschii tyrosyl-tRNA synthetase/tRNA(CUA) pair to gen

279 hyperthermophilic euryarchaeon Methanococcus jannaschii uses coenzyme M (2-mercaptoethanesulfonic aci

280 te that the Thi4 ortholog from Methanococcus jannaschii uses exogenous sulfide and is catalytic.

281 The archaeon Methanocaldococcus jannaschii uses three different 2-oxoacid elongation pat

282 MVP, a Methanococcus jannaschii voltage-gated potassium channel, was cloned a

283 presented here, aIF2beta from Methanococcus jannaschii was expressed in Escherichia coli, purified,

284 IMP cyclohydrolase activity in Methanococcus jannaschii was purified and sequenced: its genetic locus

285 Although Methanocaldococcus (Methanococcus) jannaschii was the first archaeon to have its genome seq

286 from the hyperthermophile Methanocaldococcus jannaschii, we describe a functional dissection of this

287 r Typhimurium and the archaeon Methanococcus jannaschii were purified and shown to retain structure.

288 ridium difficile, and Mj2 from Methanococcus jannaschii) were overproduced in Escherichia coli.

289 lyl-tRNA synthetase (ProRS) of Methanococcus jannaschii, which activates both proline and cysteine, t

290 ohydrolase (GCH) III from Methanocaldococcus jannaschii, which catalyzes the conversion of GTP to 2-a

291 -1.9 A) structures of PSP from Methanococcus jannaschii, which define the open state prior to substra

292 siae, and archaebacterium Methanocaldococcus jannaschii, which encodes a protein with no homology to

293 nal tyrosine pair derived from Methanococcus jannaschii, which has been used to selectively incorpora

294 ydrolase III protein from Methanocaldococcus jannaschii, which has no amino acid sequence homology to

295 of the archaeal CorA from Methanocaldococcus jannaschii, which is a unique complete structure of a Co

296 ified cell extracts of M. thermophila or Mc. jannaschii with 7,8-didemethyl-8-hydroxy-5-deazariboflav

297 cell extracts of both M. thermophila and Mc. jannaschii with [hydroxy-(18)O, carboxyl-(18)O(2)]lactat

298 erthermophilic methanogen Methanocaldococcus jannaschii with bound substrate dihydroxyacetone phospha

299 on of cell extracts of M. thermophila or Mc. jannaschii with either LPPG or LPPA and Fo generated F(4

300 eductase (RNR) using the recently evolved M. jannaschii Y-tRNA synthetase/tRNA pair.