ライフサイエンスコーパス: joining of

1 pies of its enantiomer through the templated joining of 11 component oligonucleotides.

2 Additional mechanisms can restrict joining of 12/23 RS matched segments beyond the 12/23 ru

3 We examined the joining of 3'-protruding single strand (PSS) ends, which

4 a 60S ribosomal subunit protein required for joining of 40S and 60S subunits.

5 omal subunit in 48S initiation complexes and joining of 40S and 60S subunits.

6 Joining of 48S complexes to 60S subunits to form 80S rib

7 the NHEJ-dependent and the NHEJ-independent joining of a signal end to a coding end.

8 s mutation has a dominant-negative effect on joining of a subset of DNA ends in an in vitro NHEJ assa

9 events of joining of exons, and 76 events of joining of adjacent genes as a single gene.

10 The synthesis involves convergent joining of an Eastern half and a Western half.

11 ic parabiosis, a surgical technique in which joining of animals of different ages leads to a shared c

12 ar efficiency, but addition of Ku suppresses joining of blunt ends and homologous ends with 3' overha

13 The liquid-solid joining of BMGs can shed more insights on overcoming the

14 the transpososome actively promotes coupled joining of both ends of the element DNA into the same ta

15 XLF (Cernunnos) stimulates the joining of both incompatible DNA ends and compatible DNA

16 Here we show that joining of both matched and mismatched DNA ends occurs e

17 y > 50 microM 3-aminobenzamide prevented the joining of both matched and non-homologous DNA ends.

18 e sites is coupled such that the coordinated joining of both Mu DNA ends is favored during recombinat

19 is more stable, consistent with the covalent joining of both strands of the duplex and a lower overal

20 has been suggested to occur by nonhomologous joining of broken DNA ends.

21 into two basic operations: DNA cleavage and joining of broken ends.

22 efect in Brca1 mutant cells but prevents the joining of chromatid breaks into chromosome rearrangemen

23 NHEJ repair, thus preventing deletional end-joining of chromosomal DSBs during G1.

24 ce of any known NHEJ factor severely impairs joining of cleaved V, D, and J segments.

25 Unexpectedly, trans joining of coding ends is severely impaired (100-to 1000

26 gements are initiated normally, but impaired joining of coding ends prevents assembly of functional r

27 HEJ-dependent, and not the NHEJ-independent, joining of coding ends to signal ends.

28 also needed in the later steps for efficient joining of coding ends.

29 ll extracts for the ability to stimulate the joining of cohesive DNA ends in a complementation assay

30 ally complementary overhangs, and stimulated joining of cohesive ends more than twentyfold.

31 ese proteins alone did not promote efficient joining of cohesive-ended DNA fragments in a cell-free a

32 itol hexakisphosphate (IP(6)) stimulates the joining of complementary DNA ends in a cell free system.

33 generation sequencing, de novo assembly, and joining of contigs by Sanger sequencing.

34 erly degenerate, which can physically hinder joining of contralateral heart cells leading to a broken

35 P act in the same pathway as LIG3 to promote joining of de-protected telomeres by A-NHEJ.

36 The joining of different genomes in allotetraploids played a

37 re consistent with a mechanism of cutting or joining of distal DNA lesions initiated by activation-in

38 nd breaks promotes non-homologous end-to-end joining of DNA [3].

39 he end-joining reaction was in progress, end-joining of DNA already present in the reaction continued

40 mology-directed repair and nonhomologous end joining of DNA breaks is impaired in KDM4D-deficient cel

41 ntaining genomic stability during long-range joining of DNA breaks.

42 luding translocations, require formation and joining of DNA double strand breaks (DSBs).

43 genes are also involved in nonhomologous end joining of DNA double-strand break repair, the largest s

44 sponsible for PG removal during in vitro end joining of DNA double-strand breaks (DSBs), whole-cell e

45 The accurate joining of DNA double-strand breaks by homologous recomb

46 nation mediated by Cre or non-homologous end joining of DNA double-strand breaks induced by I-Sce1, r

47 s) that is involved in the nonhomologous end joining of DNA double-strand breaks.

48 etic drugs are thought to arise by incorrect joining of DNA double-strand breaks.

49 counteracting error-prone non-homologous end joining of DNA double-strand breaks.

50 ir proteins that catalyze non-homologous end-joining of DNA double-strand breaks.

51 We conclude that joining of DNA ends during CSR does not require DNA-PKcs

52 The joining of DNA ends during Ig class-switch recombination

53 e translocations frequently form through the joining of DNA ends from double-strand breaks (DSBs) gen

54 onal chromosomal locations to facilitate the joining of DNA ends generated by other types of DSBs.

55 0 are known to be required for nonhomologous joining of DNA ends in vivo.

56 These extracts effected little joining of DNA ends with cohesive 5' or 3' overhangs, an

57 us DNA by opening chromatin and facilitating joining of DNA ends.

58 t the final steps of V(D)J recombination and joining of DNA ends.

59 op a ligase-free technology for the covalent joining of DNA fragments to suitable plasmid vectors.

60 gene that is essential for nonhomologous end joining of DNA in double-strand break repair.

61 DNA ligases catalyse the joining of DNA single- and double-strand breaks.

62 topoisomerase catalyzes the cleavage and re-joining of DNA strands through a DNA-(3'-phosphotyrosyl)

63 Notably, the joining of DNA substrates that require the combined acti

64 nd with its known role in non-homologous end joining of double strand breaks, perhaps including those

65 n is initiated by microhomology-mediated end joining of double strand breaks.

66 End joining of double-strand breaks (DSBs) requires Ku prote

67 surveyed the role of NHEJ or SSA factors in joining of double-strand breaks (DSBs) with no complemen

68 Thus the joining of double-strand breaks by mammalian extracts is

69 se Ku is required for accurate and efficient joining of double-strand breaks while similar DNA repair

70 es in V(D)J recombination, nonhomologous end-joining of double-strand breaks, and regulation of the D

71 ficiently participates in non-homologous end joining of double-strand DNA breaks.

72 is also demonstrated that Zn(2+) blocks end-joining of double-stranded (ds) DNA fragments with 3' ov

73 s no significant effect on nonhomologous end-joining of DSB ends, but it causes a significant reducti

74 DNA, has been observed in the non-homologous joining of DSB ends.

75 nduced cytidine deaminase (AID) and involves joining of DSB intermediates by end joining.

76 r DNA-PK, the initiator of nonhomologous end-joining of DSB, was involved in repair of CNDAC-induced

77 (ATM) kinase, which suppresses illegitimate joining of DSBs and activates cell-cycle checkpoints.

78 clear cells from FLT3/ITD knock-in mice, end-joining of DSBs occurs at microhomologous sequences resu

79 fecting either IgH germline transcription or joining of DSBs within S regions by classical nonhomolog

80 class switching that involves generation and joining of DSBs within two different downstream S region

81 uces phosphorylation of H2A, processing, and joining of DSBs.

82 mpairs Ku80 removal after non-homologous end joining of DSBs.

83 , we show that BS cells display aberrant end-joining of DSBs.

84 over, the Zn-f stimulates intermolecular end joining of duplexes that harbour these structures up to

85 ctions that involve distant sites, including joining of dysfunctional telomeres and AID (also known a

86 at the excursions promote non-homologous end joining of dysfunctional telomeres and implicated Nespri

87 is required for efficient non-homologous end-joining of dysfunctional telomeres.

88 RtcB catalyzes the GTP and Mn(II)-dependent joining of either 2',3'-cyclic phosphate or 3'-phosphate

89 anner identical to other Ku's and stimulated joining of ends by the host NHEJ DNA ligase (LigD).

90 Here we find that the joining of ends by XRCC4-ligase IV is markedly influence

91 Joining of ends requiring gap filling prior to ligation

92 Consistent with a mechanism involving joining of exchanged double-strand break ends, there was

93 nformation to the physical map and result in joining of existing physical map contigs into 84 cluster

94 recting the physical excision of introns and joining of exons have been elucidated in recent years, t

95 19 novel translational frames, 28 events of joining of exons, and 76 events of joining of adjacent g

96 mechanisms must exist to prevent the random joining of exons.

97 The joining of exposed 3'transposon ends to the target DNA c

98 e-specific recombinase involves breakage and joining of four DNA strands between two target substrate

99 cer-prone BALB/c mice showed inefficient end joining of gamma ray-induced DSBs as compared with cells

100 ents, random nucleotide incorporation during joining of gene segments into a complete transcript, and

101 air, RecA family proteins must promote rapid joining of homologous DNA.

102 to IgH V(D)J rearrangement to facilitate the joining of Ig variable, diversity, and joining segments.

103 Reduction of PALF in vivo reduces the joining of incompatible DNA ends.

104 also participate in imprecise rejoining and joining of incompatible ends, important mutagenic events

105 f radiation-induced cell killing, reflecting joining of incongruent DNA-ends that alter the genome.

106 53BP1 facilitates joining of intrachromosomal DSBs but only at distances c

107 consisting of 83 nucleotides, catalyses the joining of L-mono- or oligonucleotide substrates on a co

108 translation factor eIF5B, a GTPase, promotes joining of large (60S) subunits with pre-40S subunits to

109 bosomal subunit protein that is required for joining of large and small ribosomal subunits.

110 uses a highly conserved sequence (CS) in the joining of leader and body RNAs.

111 Covalent joining of leader RNA exons to pre-mRNAs by trans-splici

112 ereas polymerization is achieved by covalent joining of lysine side chains within distinct alanine-ri

113 he context of deterministic transferring and joining of materials at the microscale where surface adh

114 : filament nucleation, growth of microvilli, joining of microvillar bundles into modules, assembly of

115 sequence analysis of junctions derived from joining of mismatched DNA ends in XRCC4-deficient cells

116 complex is necessary for resection-based end joining of mismatched DNA ends.

117 n through double strand breaks (DSB) and the joining of newly excised transposon ends with target DNA

118 sphorylation provides one route by which end-joining of non-homologous DNA can be regulated.

119 ase's endonuclease activity in promoting the joining of noncompatible ends.

120 nction with a DNA ligase, Mre11 promotes the joining of noncomplementary ends in vitro by utilizing s

121 and Xenopus eggs possess activities for the joining of nonhomologous DNA ends, and such activities m

122 The synthesis, processing, and joining of Okazaki fragments during DNA replication is c

123 iding clamp, co-ordinates the processing and joining of Okazaki fragments during eukaryotic DNA repli

124 gase I/PCNA interaction and suggest that the joining of Okazaki fragments is coordinated by pairwise

125 gase I to replication foci and the efficient joining of Okazaki fragments is dependent on the interac

126 Flap endonuclease 1 (FEN1), involved in the joining of Okazaki fragments, has been proposed to restr

127 NA ligase I that complete the processing and joining of Okazaki fragments, respectively.

128 in-protein interface that may coordinate the joining of Okazaki fragments.

129 ts that other factors may be involved in the joining of Okazaki fragments.

130 e one active site promotes both cleavage and joining of one Mu DNA end.

131 sposon end: cleavage of the two strands plus joining of one strand to target DNA.

132 We report here that end joining of P-element breaks in the absence of DmRad51 do

133 rnunnos protein restored gap filling and end joining of partially complementary overhangs, and stimul

134 olves separate transcription of gene pieces, joining of precursor RNAs via trans-splicing, and RNA ed

135 tive forms that lead to correct or incorrect joining of products.

136 nation, MRN deficiency leads to the aberrant joining of Rag DSBs and to the accumulation of unrepaire

137 proach relies on the ordering, trimming, and joining of randomly cleaved parental DNA fragments annea

138 such as iterative recalibration and neighbor joining of reads to identify enriched regions of any len

139 st 2 decades have revealed that the aberrant joining of replication-associated breaks leads to catast

140 omosome stability by promoting efficient end joining of replication-derived breaks, as well as TLS an

141 ation of these pathways using as a model end joining of restriction endonuclease linearized plasmid D

142 vitro, extracts of 180BR cells supported end joining of restriction endonuclease-digested plasmid to

143 RNA enzyme that catalyzes the RNA-templated joining of RNA was converted to a format whereby two enz

144 These proteins are important for faithful joining of S regions, and in their absence aberrant reco

145 bulins and T-cell receptors by combinatorial joining of segments of coding DNA.

146 Here, we show that both trans cleavage and joining of signal ends occur efficiently in vivo.

147 nd Dnl4-Lif1 is much more efficient than the joining of similar DNA substrates that require only liga

148 DNA ligases catalyse the joining of single and double-strand DNA breaks, which is

149 ein in vitro have supported mainly uncoupled joining of single cDNA ends.

150 Moreover, joining of SmaI-generated blunt ends is generally imprec

151 alysis of the switch fragments showed direct joining of Smu and Sgamma3 without deletions or duplicat

152 ands A or G and that disulfide bond-mediated joining of strand A to the core strand B cooperatively s

153 DNA ligases catalyze the joining of strand breaks in the phosphodiester backbone

154 This may include non-homologous end-joining of strand breaks resulting from DNA damage, beca

155 iation factor IF2 (re and, like it, mediates joining of subunits and has a ribosome-dependent GTPase

156 reaks and, consistent with this result, that joining of such breaks is specifically decreased in cell

157 acting NHEJ and a slow-acting alterative end joining of switch region breaks during CSR.

158 Cloning DNA typically involves the joining of target DNAs with vector constructs by enzymat

159 shorten telomeric DNA, and nonhomologous end-joining of telomeric DNA, can lead to loss of telomere f

160 ' end of the viral DNA; strand transfer, the joining of the 3' ends of viral DNA to host DNA; and 5'-

161 ve site in TnsB also mediates the subsequent joining of the 3' ends to the target.

162 DNA; and 5'-end joining (or gap repair), the joining of the 5' ends of viral DNA to host DNA.

163 hydrolysis of bound GTP with the concomitant joining of the 60 S ribosomal subunit to the 40 S initia

164 ctor 5B (eIF5B) is a GTPase that facilitates joining of the 60 S ribosomal subunit to the 40 S riboso

165 f the 48S preinitiation complex, followed by joining of the 60S ribosomal subunit and formation of th

166 A second GTPase, eIF5B, catalyzes the joining of the 60S subunit to produce an 80S initiation

167 ion to the site for editing does not require joining of the amino acid to the nucleic acid.

168 that these phenomena may reflect defects in joining of the broken DNA ends or in protection of the e

169 The later steps involve processing and joining of the cleaved DNA ends, and until now have been

170 Joining of the donor DNA to the acceptor DNA is detected

171 trong promoter for expression of mooV by the joining of the ends of the insertion sequence element at

172 end of Tn7 and target insertion results from joining of the exposed 3' Tn7 tips to the target DNA.

173 s/Delta)(TM) splice variant results from the joining of the first and third exons with skipping of th

174 om a protein, accompanied by the concomitant joining of the flanking polypeptide sequences, the extei

175 eir excision from precursor proteins and the joining of the flanking protein sequences (exteins).

176 on from a precursor protein with concomitant joining of the flanking sequences.

177 Joining of the fragments by DNA ligase I to generate the

178 the mouse SCID mutation in DNA-PKCS disrupts joining of the hairpin coding ends but spares joining of

179 generated in large part by the combinatorial joining of the Ig gene segments, VH, D, and JH, that tog

180 Upon joining of the large and small ribosomal subunits, a 100

181 ryotic translational initiation involves the joining of the large and small subunits of the ribosome,

182 Joining of the large, 50S, ribosomal subunit to the smal

183 oining of the hairpin coding ends but spares joining of the open signal ends.

184 Subsequent joining of the overhand knot end groups by ring-closing

185 ends of viral DNA, and sequence-independent joining of the recessed viral ends to staggered phosphat

186 Spatially coherent break-up leads to the joining of the spheres into a bispherical silicon 'p-n m

187 nitiator RNA primer that is removed prior to joining of the strands.

188 We characterized the abnormal joining of the telencephalon to the diencephalon and def

189 inked by a protein bridge, since coordinated joining of the two ends is not disrupted by cleaving the

190 egration of retroviral cDNA involves coupled joining of the two ends of the viral genome at precisely

191 egion suggests a key role in stabilizing the joining of the two helical domains, and in maintaining t

192 e-strand break could be healed by (i) direct joining of the two partial genomes resulting from the br

193 both adenylation of the 5'-probe strand and joining of the two probe strands.

194 The joining of the two viral DNA ends to target DNA occurs s

195 The final joining of these blunt ends followed the same kinetics a

196 end of the element genome and the subsequent joining of these cleaved ends to a new target DNA site.

197 The joining of these regions with neighboring segments, wher

198 d junctions that are typically formed by the joining of three to four of the linear segments.

199 However, direct fusion joining of Ti6Al4V to SS316 can cause brittle Ti-Fe inte

200 The covalent joining of topoisomerases to DNA is normally a transient

201 RNL and Bf PNK/CPDase are sufficient for the joining of tRNA splicing intermediates in vitro, and for

202 A method is described for the joining of two alpha-lithiated C(sp(3)) stereocenters ef

203 mutants and most aberrations reflect either joining of two broken chromosomes in a "half crossover"

204 A critical overview of the catalytic joining of two different electrophiles, cross-electrophi

205 egitimate recombination characterized by the joining of two DNA ends that lack homology.

206 s an intermolecular beta-sheet formed by the joining of two individual GFC beta-sheets and a large bu

207 RP biogenesis culminates with the joining of two large subcomplexes, the lid and base.

208 on, we used parabiotic pairing, ie, surgical joining of two mice, to create a shared circulation betw

209 s linked to an RNA enzyme that catalyzes the joining of two oligonucleotide substrates.

210 important phenomenon involving the physical joining of two plants to generate a chimeric organism.

211 orphyrin pentamer + porphyrin monomer or the joining of two porphyrin trimers.

212 otides, which catalyse the template-directed joining of two RNA molecules, one bearing a 5'-triphosph

213 h acting in end resection, also promotes end-joining of uncapped telomeres.

214 ni from DNA double-strand break ends and the joining of unmodified ends.

215 e successful deletion and non-homologous end joining of up to 725 kb reframed the DMD gene.

216 0-deficient cells were severely impaired for joining of V(D)J coding and recombination signal sequenc

217 Direct joining of Vbeta segments to nonrearranged Dbeta or Jbet

218 eta to Jbeta rearrangements occurring before joining of Vbetas to a DJbeta complex.

219 Adhesive joining of veneers to cores offers potential simplicity

220 dings, we suggest that (a) nonhomologous end joining of Vlambda1 and Jlambda1 coding ends in fetal B

221 Such frequent joining of widely separated CSR DSBs could be promoted b

222 t a general DNA repair process that promotes joining of widely separated DSBs within a chromosome.

223 nerating chimeric animals through parabiotic joining of wild-type (wt) and diabetic (db/db) mice.