ライフサイエンスコーパス: kDNA

1 kDNA decatenation assay indicated that XWL-1-48 signific

2 kDNA synthesis involves release of individual minicircle

3 kDNA, the mitochondrial genome of trypanosomatids, is a

4 Database searching for a kDNA replicative polymerase (pol) revealed no mitochondr

5 RNAi depletion of PNT1 in a kDNA independent cell line resulted in kinetoplast loss

6 binding domain of AEP-1 results in aberrant kDNA structure and reduced cell growth, indicating that

7 poisomerase II interaction (DNA cleavage and kDNA decatenation assays), alongside cytotoxicity tests

8 rsal minicircle sequence-binding protein and kDNA loss.

9 measured by real-time qPCR: OligoC-TesT and kDNA PCR detected 100% and 99% of positive samples when

10 d their knockdown by RNA interference caused kDNA network shrinkage.

11 This overexpression caused kDNA loss by oxidation/inactivation of the universal min

12 ecombinant proteins is capable of compacting kDNA networks in vitro and was shown to bind preferentia

13 ciated proteins in organizing and condensing kDNA networks into this disc structure, we have cloned t

14 lear extracts containing Metnase decatenated kDNA more rapidly than those without Metnase, and neutra

15 At later times following TbLOK1 depletion, kDNA was lost and a more drastic alteration in mitochond

16 PCR detected kDNA more frequently in biopsies (detection level, 83.9%

17 ts localization patterns vary with different kDNA replication stages.

18 Kinetoplast DNA (kDNA) is a novel form of mitochondrial DNA consisting of

19 Kinetoplast DNA (kDNA) is organized into a concatenated network of mini a

20 whole linearized minicircle kinetoplast DNA (kDNA) of the Leishmania subgenus Viannia from biopsy lys

21 C-TesT with those of nested kinetoplast DNA (kDNA) PCR, nested internal transcribed spacer 1 (ITS-1)

22 The multicopy kinetoplast DNA (kDNA) probes were the most sensitive and useful for quan

23 ome of trypanosomes, termed kinetoplast DNA (kDNA), contains thousands of minicircles and dozens of m

24 Kinetoplast DNA (kDNA), from trypanosomatid mitochondria, is a network co

25 osome mitochondrial genome, kinetoplast DNA (kDNA), is a massive network of interlocked DNA rings, in

26 This genome, known as kinetoplast DNA (kDNA), is organized as a single, massive DNA network for

27 able DNA structure known as kinetoplast DNA (kDNA), isolated from a parasite.

28 ion of RNAi was the loss of kinetoplast DNA (kDNA), the cell's catenated mitochondrial DNA network.

29 Kinetoplast DNA (kDNA), the form of mitochondrial DNA in trypanosomatids,

30 Kinetoplast DNA (kDNA), the mitochondrial DNA in kinetoplastids, is a net

31 Kinetoplast DNA (kDNA), the mitochondrial DNA of trypanosomatids, consist

32 Kinetoplast DNA (kDNA), the trypanosome mitochondrial DNA, contains thous

33 Kinetoplast DNA (kDNA), the trypanosome mitochondrial genome, is a giant

34 Kinetoplast DNA (kDNA), the unusual mitochondrial DNA of Trypanosoma bruc

35 library screen for loss of kinetoplast DNA (kDNA), we identified an uncharacterized Trypanosoma bruc

36 cles and maxicircles called kinetoplast DNA (kDNA).

37 DNA molecules that form the kinetoplast DNA (kDNA).

38 mitochondrial DNA known as kinetoplast DNA (kDNA).

39 not (decatenate) and cleave kinetoplast DNA (kDNA).

40 not (decatenate) and cleave kinetoplast DNA (kDNA).

41 ns a kinetoplast with the mitochondrial DNA (kDNA), comprising of >70% AT base pairs.

42 For example, kDNA replication without segregation causes the networks

43 dy in intact cells and in isolated flagellum-kDNA complexes.

44 Following kDNA replication, the TAC mediates network segregation,

45 the molecular mechanism of compensation for kDNA loss by showing FO-independent generation of the mi

46 ce HMG box-containing proteins essential for kDNA function from their kDNA binding sites.

47 ergetically demanding apparatus required for kDNA maintenance and expression serves the production of

48 Additionally, a defect in kDNA replication was observed in the 2N1K cells.

49 F8 arrests cell growth and causes defects in kDNA segregation.

50 mitochondrial architecture that function in kDNA replication.

51 seven DNA polymerases (pols) are involved in kDNA transactions, including three essential proteins re

52 f type II topoisomerases that participate in kDNA metabolism (we term the T. brucei brucei gene TbTOP

53 ycle suggests that other processes involving kDNA occur in this domain.

54 intenance and expression of its kinetoplast (kDNA), the mitochondrial genome of this parasite and a p

55 RNAi of TbPIF8 causes only limited kDNA shrinkage but the networks become disorganized.

56 Within the mitochondrion, kDNA is condensed into a disk-shaped structure positione

57 Unlike all other DNA in nature, kDNA comprises a giant network of interlocked DNA rings

58 erence leads to pleomorphic nuclear (but not kDNA) abnormalities and early growth arrest.

59 by RNAi there is a striking accumulation of kDNA late theta structure replication intermediates, wit

60 The level of decatenation activity of kDNA was comparable in nuclear extracts from control or

61 The unusual complexity of kDNA would indicate that numerous proteins must be invol

62 The detection of kDNA in biopsies from chronic lesions was enhanced by a

63 TbPIF8 is positioned on the distal face of kDNA disk and its localization patterns vary with differ

64 cle-dependent changes in the localization of kDNA replication enzymes by combining immunofluorescence

65 can compensate for complete physical loss of kDNA in these parasites.

66 ation intermediates, with subsequent loss of kDNA networks and halt in cell growth.

67 in growth inhibition and progressive loss of kDNA networks.

68 The loss of kDNA was preceded by gradual shrinkage of the network an

69 iability and is important for maintenance of kDNA.

70 their mode of action with diverse methods of kDNA decatenation, DNA-Topo cleavage complex, comet, DNA

71 ania life stages retained similar numbers of kDNA maxi- or minicircles.

72 ected in the antipodal sites at the onset of kDNA replication.

73 olved in the organization and segregation of kDNA networks in trypanosomatids.

74 , and its discovery will facilitate study of kDNA segregation machinery at the molecular level.

75 o complete the topologically complex task of kDNA replication is unknown.

76 nterval [CI], 63 to 78%), similar to that of kDNA PCR (72%; 95% CI, 65 to 80%; P = 0.69) but signific

77 ts on replication enzymes, how the timing of kDNA synthesis is controlled during the cell cycle, and

78 I decided to take a chance on kDNA.

79 RNAi of p166 has only small effects on kDNA replication, but it causes profound defects in netw

80 In this review, we discuss recent studies on kDNA structure and replication, emphasizing recent devel

81 intricate mitochondrial DNA (kinetoplast or kDNA) in the form of a network of thousands of interlock

82 intricate mitochondrial DNA (kinetoplast or kDNA) of Trypanosoma brucei brucei and related kinetopla

83 ng the mitochondrial genome of the parasite (kDNA), with an accumulation of the protein at or near th

84 To test their validity for quantification, kDNA copy numbers were compared between Leishmania speci

85 the next forty years of my life to studying kDNA replication.

86 the inner unilateral filaments adjoining the kDNA disc.

87 TbPOLIB and TbPOLIC localized beside the kDNA where replication occurs, and their knockdown by RN

88 vered p166, a protein localizing between the kDNA and basal body in intact cells and in isolated flag

89 alizes to the region of the cell between the kDNA and the flagellum and purifies with the tripartite

90 Most genes encoded by the kDNA require a posttranscriptional modification process

91 en localized to antipodal sites flanking the kDNA disk along with nascent DNA minicircles.

92 localize in two antipodal sites flanking the kDNA during replication, they behave differently at othe

93 e enzyme in two antipodal sites flanking the kDNA, show that a function of this topoisomerase II is t

94 a structure believed to physically link the kDNA and flagellar basal bodies.

95 ucture rich in basic proteins that links the kDNA discs during their segregation and is maintained be

96 a link between the unusual structure of the kDNA and RNA editing in trypanosome mitochondria.

97 itochondrial matrix at opposite edges of the kDNA disc.

98 In Crithidia fasciculata, rotation of the kDNA disk relative to the antipodal attachment sites res

99 nicircles accumulate on opposite ends of the kDNA disk, a pattern that did not suggest kinetoplast mo

100 podal sites and also at the two faces of the kDNA disk.

101 The minicircles replicate free of the kDNA network but nicks and gaps in the newly synthesized

102 The replication of the kDNA network is more complex than previously thought, an

103 Based on fluorescence microscopy of the kDNA network undergoing replication, we now report that

104 per, I found the electron micrographs of the kDNA networks to be rather beautiful.

105 AEP-1 is involved in the maintenance of the kDNA.

106 hods and appear to be present throughout the kDNA.

107 alizes within the mitochondrion close to the kDNA disk in patterns that vary with the cell cycle.

108 ds remain at the time of reattachment to the kDNA network.

109 16 shows that p16 is present both within the kDNA disc and in the mitochondrial matrix at opposite ed

110 teins essential for kDNA function from their kDNA binding sites.

111 important when I learned that parasites with kDNA threaten huge populations in underdeveloped tropica

112 I had no doubt that working with kDNA would be a challenge, as I would be exploring uncha