ライフサイエンスコーパス: karyopherin

1 ase of Ran from nuclear transport receptors (karyopherins).

2 acterized yeast protein Nmd5p functions as a karyopherin.

3 calization and interaction with its relevant karyopherins.

4 taining Nups that serve as docking sites for karyopherins.

5 nucleoporins, general transport factors, and karyopherins.

6 ted by cognate nuclear transport factors, or karyopherins.

7 he adaptor nucleoporins arose from ancestral karyopherins.

8 ing different sequence motifs and dissimilar karyopherins.

9 asis of limited sequence similarity to known karyopherins.

10 complex, the small GTPase Ran, and cellular karyopherins.

11 and dissociation of ribosomal proteins from karyopherins.

12 the majority of binding energy for all five karyopherins.

13 to FG-repeat-containing nucleoporins through karyopherins.

14 blocks nuclear import mediated by different karyopherins.

15 he nucleus using the cellular alpha and beta karyopherins.

16 ucleus by the Karyopherin beta family member Karyopherin 13 (Kap13).

17 IFN) signaling by binding to NPI-1 subfamily karyopherin alpha (KPNA) nuclear import proteins, preven

18 its interaction with the NPI-1 subfamily of karyopherin alpha (KPNA) nuclear transporters.

19 AT1 (PY-STAT1), which occurs via a subset of karyopherin alpha (KPNA) nuclear transporters.

20 of Ebola virus (EBOV) VP24 protein with host karyopherin alpha (KPNA) proteins blocks type I interfer

21 We analyzed the role of karyopherin alpha (KPNA), a key classical nuclear import

22 ng sites/regions in Saccharomyces cerevisiae karyopherin alpha (SRP1).

23 own assays indicate interaction of APE1 with karyopherin alpha 1 and 2, which requires the 20 N-termi

24 uclear import complex formation by tethering karyopherin alpha 2 and karyopherin beta 1 to the membra

25 t mutations in the C terminus no longer bind karyopherin alpha 2 or block the nuclear import of STAT1

26 We also show that N-terminal deletions of karyopherin alpha 2 that no longer bind to karyopherin b

27 mbinant SARS-CoV lacking ORF6 did not tether karyopherin alpha 2 to the ER/Golgi membrane and allowed

28 We mapped the region of ORF6, which binds karyopherin alpha 2, to the C terminus of ORF6 and show

29 ns that interact with the NLS and identified karyopherin alpha 3 (KPNA3 or Kap-alpha3) and karyopheri

30 aryopherin alpha 3 (KPNA3 or Kap-alpha3) and karyopherin alpha 4 (KPNA4 or Kap-alpha4) as key binding

31 g as an 'adapter' molecule between VirE2 and karyopherin alpha and 'piggy-backing' VirE2 into the hos

32 Interaction of karyopherin alpha and a classical nuclear localization s

33 heterodimeric receptor comprised of importin/karyopherin alpha and beta.

34 dimeric import receptor composed of importin/karyopherin alpha and beta.

35 nteraction with all six members of the human karyopherin alpha family.

36 This association increased the affinity of karyopherin alpha for basic-type NLSs, including that of

37 nal significance of specific residues within karyopherin alpha for NLS cargo binding.

38 monstration that a glutathione S-transferase-karyopherin alpha fusion interacts with ORF29p, but not

39 We have also expressed each karyopherin alpha mutant in vivo as the only cellular co

40 We established that five karyopherin alpha paralogs are expressed by primary mous

41 the Bag6 nuclear localization sequence from karyopherin alpha to retain Bag6 in the cytosol but also

42 A karyopherin alpha variant with a mutation in the major N

43 However, we also find that a karyopherin alpha variant with a mutation in the minor N

44 ed to the creation of conditional alleles of karyopherin alpha with well characterized defects in NLS

45 factor Srp1 (also known as importin alpha or karyopherin alpha) is required for ubiquitin-independent

46 egulatory role in this process by binding to karyopherin alpha, a cellular receptor for nuclear local

47 ions as a molecular bridge between VirE2 and karyopherin alpha, allowing VirE2 to utilize the host ce

48 ure of a 50 kDa fragment of the 60 kDa yeast karyopherin alpha, in the absence and presence of a mono

49 In addition to binding plant karyopherin alpha, VirE3 interacts with VirE2, a major b

50 p is transported into the nucleus by a Ran-, karyopherin alpha- and beta-dependent mechanism.

51 l and then imported into its nucleus via the karyopherin alpha-dependent pathway.

52 beta2 or transportin, and does not require a karyopherin alpha-like adapter protein.

53 ing is also involved in the association with karyopherin alpha.

54 nt of the cellular nuclear import machinery, karyopherin alpha.

55 S cargo that is imported into the nucleus by karyopherin alpha.

56 x and is modulated through interactions with karyopherin alpha.

57 which have been termed importin alpha/beta, karyopherin alpha/beta, or PTAC 58/ 97.

58 We found that yeast karyopherin alpha/Srp1p and Crm1p are required for the n

59 phorylated and imported into the nucleus via karyopherin alpha/Srp1p.

60 a cytoplasmic receptor such as the importin (karyopherin)-alpha subunit.

61 lves of the ring are structurally related to karyopherin-alpha (Kap-alpha) and beta-karyopherin famil

62 e preferential interaction of W protein with karyopherin-alpha 3 and karyopherin-alpha 4.

63 on of W protein with karyopherin-alpha 3 and karyopherin-alpha 4.

64 nsportation of DP rcDNA via interaction with karyopherin-alpha and -beta.

65 ed with cellular nuclear transport receptors karyopherin-alpha and -beta.

66 hese sequences can mediate direct binding to karyopherin-alpha and are essential for the passage of i

67 cargoes and their import receptor proteins, karyopherin-alpha and karyopherin-beta, can be robustly

68 Furthermore, karyopherin-alpha, karyopherin-beta1 and the Ran GTPase

69 (pendulin/Rch1/alpha-P1/hSrp1alpha and Srp1/karyopherin-alpha/alpha-S1/NPI-1) which function in nucl

70 x 10(-)(3)), and the nuclear import protein karyopherin alpha1 (KPNA [rs6810306]; P = 4.91 x 10(-)(2

71 es, that PY-STAT1 can interact not only with karyopherin alpha1 but also with karyopherins alpha5 and

72 -STAT1 interaction, indicating that the VP24-karyopherin alpha1 interaction contributes to the block

73 VP24 is found to specifically interact with karyopherin alpha1, the nuclear localization signal rece

74 Overexpression of VP24 results in a loss of karyopherin alpha1-PY-STAT1 interaction, indicating that

75 with overexpressed but also with endogenous karyopherin alpha1.

76 binding to both overexpressed and endogenous karyopherin alpha1.

77 binding region located in the C terminus of karyopherin alpha1.

78 r localization signal receptor for PY-STAT1, karyopherin alpha1.

79 ound and inhibited PY-STAT1 interaction with karyopherins alpha1, alpha5, and alpha6.

80 t VP24 inhibits interaction of PY-STAT1 with karyopherins alpha1, alpha5, or alpha6 by binding within

81 apable of binding its nuclear import adaptor karyopherin-alpha1 (KPNA1).

82 red that Nsp1beta induced the degradation of karyopherin-alpha1 (KPNA1, also called importin-alpha5),

83 y interacted with the nuclear import protein karyopherin-alpha1 but not with karyopherin-alpha2, -3,

84 ryopherin-alpha2, -3, or -4, suggesting that karyopherin-alpha1 transports nsP2 to the nucleus during

85 We found that BPV1 L2 bound to the karyopherin alpha2 (Kap alpha2) adapter and formed a com

86 VP1 and L1 capsomeres could bind both karyopherin alpha2 and DNA simultaneously.

87 n signal receptor for PY-STAT1, but not with karyopherin alpha2, alpha3, or alpha4.

88 La cells using the recombinant human factors karyopherin alpha2, karyopherin beta1, Ran, and p10.

89 nor a mixture of recombinant import factors (karyopherin alpha2, karyopherin beta1, Ran, and p10/NTF2

90 ation, and its interaction with the importin karyopherin alpha2.

91 port protein karyopherin-alpha1 but not with karyopherin-alpha2, -3, or -4, suggesting that karyopher

92 Capsomeres of L1, but not VP1, bound by karyopherin alpha2beta1 or beta1 alone were unable to bi

93 Both VP1 and L1 capsomeres bound by karyopherin alpha2beta1 were unable to assemble into cap

94 we found that only one of the NLS receptors, karyopherin alpha3 (Kapalpha3/Qip), would support signif

95 Mutating the nuclear importer karyopherin alpha3 also leads to reduced toxicity from p

96 ng of a Drosophila nuclear transport protein karyopherin-alpha3 (dKap-alpha3).

97 Here we report a novel correlation between karyopherin alpha4 (KPNA4) and PCa pathological stages.

98 ) are likely to impair VP24 binding to human karyopherin alpha5 (KPNA5) and therefore inhibition of i

99 ations located in the protein interface with karyopherin alpha5 may enable VP24 to inhibit karyopheri

100 t only with karyopherin alpha1 but also with karyopherins alpha5 and alpha6, which together comprise

101 termined that importin alpha7, also known as karyopherin alpha6 (KPNA6), directly interacts with the

102 imported into the plant cell nucleus via the karyopherin alphadependent pathway and that elevated int

103 ich together comprise the NPI-1 subfamily of karyopherin alphaS.

104 ransferase pull-down assays, TDP-43 bound to karyopherin-alphas, thereby confirming the classical nuc

105 Sxm1p and Lhp1p represent the karyopherin and a cognate substrate of a unique nuclear

106 domain, we examined the relationship between karyopherin and DNA binding of both mPy VP1 and HPV11 L1

107 Knowledge of the cargoes carried by each karyopherin and insight into the mechanisms of transport

108 t from the competition by importin-beta/beta-karyopherin and may be involved in the physiological reg

109 nucleoporin mutants as well as a few of the karyopherin and transport factor mutants also mislocaliz

110 FG domain may have the capacity to bind both karyopherins and an mRNA export factor simultaneously.

111 we show that Bcp1 dissociates Rpl23 from the karyopherins and associates with Rpl23 afterward.

112 L2 interacts via its NLSs with a network of karyopherins and can enter the nucleus via several impor

113 potent modifiers of DPR toxicity, including karyopherins and effectors of Ran-mediated nucleocytopla

114 importin-beta alone or in complex with other karyopherins and enters the nucleus via the NPC.

115 nteracts via its C-terminal NLS with several karyopherins and exploits these interactions to enter th

116 lalanine-glycine repeats (FG Nups) that bind karyopherins and facilitate the transport of karyopherin

117 s identified by mass spectrometry, most were karyopherins and nucleoporins.

118 aryopherin alpha5 may enable VP24 to inhibit karyopherins and subsequently the host interferon respon

119 ng within the PY-STAT1 binding region of the karyopherins and that this function is conserved among t

120 Thus, HPV11 L2 can interact with several karyopherins and the viral DNA and may enter the nucleus

121 s were used to investigate the role of other karyopherins, and the results suggested that rAAV2 may u

122 opose that, in the absence of Kap123p, these karyopherins are able to supplant Kap123p's role in impo

123 These results support a role for karyopherins as chaperones in the in vivo regulation of

124 human papillomavirus type 11 (HPV11) L2 with karyopherin beta (Kap beta) nuclear import receptors rev

125 f karyopherin alpha 2 that no longer bind to karyopherin beta 1 still retain ORF6 binding activity bu

126 rmation by tethering karyopherin alpha 2 and karyopherin beta 1 to the membrane.

127 tor Pax6 is imported into the nucleus by the Karyopherin beta family member Karyopherin 13 (Kap13).

128 p and not with other members of the importin/karyopherin beta superfamily.

129 nterfering RNA screens previously identified karyopherin beta transportin-3 (TNPO3) and NPC component

130 We found that the karyopherin beta-related export receptor CRM1 competes w

131 n via a classical import mechanism utilizing karyopherin beta.

132 ptor complex comprised of the Rch1/importin (karyopherin)-beta heterodimer expressed in Jurkat T cell

133 Transport factors in the karyopherin-beta (also called importin-beta) family medi

134 structural basis for the specificity of the karyopherin-beta family for the GTP-bound state of Ran,

135 ne pSTAT3 translocates to the nucleus by the karyopherin-beta nucleocytoplasmic system and binds DNA.

136 y soluble nuclear transport receptors of the karyopherin-beta superfamily termed importins and export

137 ighly specific inhibitor of CRM1, a cellular karyopherin-beta that transports nuclear export signal-c

138 f full-length yeast importin-beta (Kap95p or karyopherin-beta) complexed with RanGTP, which provides

139 ort receptor proteins, karyopherin-alpha and karyopherin-beta, can be robustly measured and that quan

140 madillo and Heat repeats of beta-catenin and karyopherin-beta, respectively.

141 ts show that FG domain collapse is caused by karyopherin beta1 (Kapbeta1) binding at low concentratio

142 Binding of GTP-bound Ran (RanGTP) to karyopherin beta1 (Kapbeta1) releases import cargo into

143 nuclear import of pA-RCC1 also required both karyopherin beta1 and Ran.

144 also capable of import through the importin/karyopherin beta1 pathway but was not functional in all

145 ecombinant human factors karyopherin alpha2, karyopherin beta1, Ran, and p10.

146 ombinant import factors (karyopherin alpha2, karyopherin beta1, Ran, and p10/NTF2) were able to suppo

147 e M9 NLS binds a transport factor related to karyopherin beta1, termed karyopherin beta2 or transport

148 that the transport of the classical receptor karyopherin-beta1 (Kapbeta1) is regulated so as to produ

149 clear transport and found that knockdowns of karyopherin-beta1 and cellular apoptosis susceptibility

150 Furthermore, karyopherin-alpha, karyopherin-beta1 and the Ran GTPase cycle are required

151 a1 mRNA or expression of a dominant-negative karyopherin-beta1 in a stable cell line supporting HBV r

152 nsfection of small interfering RNA targeting karyopherin-beta1 mRNA or expression of a dominant-negat

153 Karyopherin beta2 (Kapbeta2, transportin) binds the M9 s

154 Karyopherin beta2 or transportin recognizes a proline-ty

155 factor related to karyopherin beta1, termed karyopherin beta2 or transportin, and does not require a

156 uman NXF1 can be imported via importin beta, karyopherin beta2, importin 4, importin 11, and importin

157 hromatography, we show that huntingtin has a karyopherin beta2-dependent proline-tyrosine (PY)-NLS in

158 oxidative stress-dependent interaction with karyopherin beta2.

159 the PY-NLS, that is recognized by the human karyopherin beta2/transportin (Kapbeta2) receptor.

160 nown pathways of protein nuclear import, the karyopherin beta2/transportin pathway is only the second

161 Karyopherin-beta2 (transportin) binds a cognate import s

162 In the nucleus, Ran x GTP binds karyopherin-beta2 and dissociates the substrate.

163 Karyopherin-beta2 contains eighteen HEAT repeats arrange

164 an GTPase with a Ran-binding domain and with karyopherin-beta2 have revealed unusually tight embraces

165 rt receptor transportin 1 (Trn1; also called karyopherin-beta2) in an RNA-sensitive manner.

166 Here we present the 3.0 A structure of the karyopherin-beta2-Ran x GppNHp complex where GppNHp is a

167 , compared to Ran GDP, in regions contacting karyopherin-beta2.

168 ith karyopherins; each bound 6--10 different karyopherin betas, including importins as well as export

169 of the RanGTP gradient and redistribution of karyopherins between the nucleus and the cytoplasm.

170 ing that beta-catenin and importin-beta/beta-karyopherin both interact with common nuclear pore compo

171 h the ability to form ternary complexes with karyopherins, but with the capacity to potentiate RanGAP

172 Indeed, each karyopherin can interact directly with ELYS.

173 s bearing nuclear transport receptors called karyopherins can exhibit behaviour that varies from high

174 de interactions that resemble those found in karyopherin*cargo complexes and support the proposal tha

175 karyopherins and facilitate the transport of karyopherin-cargo complexes.

176 occurs in seconds and involves assembly of a karyopherin.cargo complex and docking to the NPC, transl

177 en the ectopic expression of Dalpha1 and the karyopherins CAS and importin beta1 suggest that high nu

178 s a Brownian ratchet model, in which a cargo-karyopherin complex remains bound to the same FG-Nups fo

179 ed hydrophobic residues that bind the export karyopherin CRM1.

180 pression attenuates the activity of numerous karyopherin-dependent host transcription factors (VDR, C

181 hin nuclear pore complexes (NPCs) to disrupt karyopherin-dependent nuclear-cytoplasmic transport and

182 leoporin that binds import- and export-bound karyopherins, dynamically associates with the NPC in a R

183 Nup116p exhibited generic interactions with karyopherins; each bound 6--10 different karyopherin bet

184 Transportin-SR2 (Tnpo3, TRN-SR2), a human karyopherin encoded by the TNPO3 gene, has been identifi

185 thways and readily saturated, is the nuclear karyopherin exportin-5.

186 ed to karyopherin-alpha (Kap-alpha) and beta-karyopherin family members.

187 We have identified the karyopherin family of nuclear import factors as possible

188 Crm1 is a member of the karyopherin family of nucleocytoplasmic transport recept

189 e that one of importin beta's relatives, the karyopherin family of proteins, manages this checkpoint.

190 establish importin-11 as a new member of the karyopherin family of transport receptors, and identify

191 ansport factors that are members of the beta-karyopherin family, which can bind cargo directly (e.g.,

192 oluble receptors of the importin/exportin or karyopherin family.

193 The flexible beta-karyopherin fold of Tnpo3 embraces the RNA recognition m

194 Srp1, Kap95, and Sxm1 as the most important karyopherins for Rrp6 nuclear import and the nuclear loc

195 irus type 11 (HPV11) L1 capsomeres bound the karyopherin heterodimer alpha2beta1 in vitro in a nuclea

196 The yeast karyopherin heterodimer Kap60p.Kap95p facilitates nuclea

197 TRN-SR2, Transportin-3, TNPO3) is a cellular karyopherin implicated in nuclear import of HIV-1.

198 meric import receptor consisting of the beta-karyopherin importin beta, which mediates interactions w

199 ld-type, passive export rates of a classical karyopherin/importin (Kap) Kap60p/Kap95p-targeted NLS-GF

200 calization signal (NLS) to the NLS receptor, karyopherin/importin alpha, is the most well studied nuc

201 ere we demonstrate that Kap114p, the primary karyopherin/importin responsible for the nuclear import

202 1p can bind directly to Kap114p, the primary karyopherin/importin responsible for the nuclear import

203 ross the nuclear envelope most frequently by karyopherin/importin-beta superfamily members that are c

204 of CD40 into the nucleus through the classic karyopherins (importins-alpha/beta) pathway.

205 rins) and soluble nuclear transport factors (karyopherins, importins, and exportins).

206 ulation is enhanced by addition of exogenous karyopherins/importins or RCC1, both of which also enhan

207 stones H3 and H4 is mediated by at least two karyopherins/importins, Kap123p and Kap121p.

208 , the functional relevance and regulation of karyopherins in hepatocellular carcinoma (HCC) is poorly

209 is controlled by varying the amount of free karyopherins in solution, which modulates the multivalen

210 play a role in the release of histones from karyopherins in the nucleus.

211 specifically competed by importin-beta/beta-karyopherin, indicating that beta-catenin and importin-b

212 portin-beta family and found that all tested karyopherins invert their subcellular distributions upon

213 The structural flexibility of beta-karyopherins is critical to mediate the interaction with

214 t nuclear import of TBP is mediated by a new karyopherin (Kap) (importin) family member, Kap114p.

215 We found that HPV16 E6 interacted with the karyopherin (Kap) alpha2 adapter and could enter the nuc

216 We have identified a novel human karyopherin (Kap) beta family member that is related to

217 ction by modulating protein interactions for karyopherin (Kap) beta family members.

218 e MSN5 was previously shown to function as a karyopherin (Kap) for nuclear export of various proteins

219 This gene product functions as a karyopherin (Kap) for nuclear import.

220 imported into the nucleus by members of the karyopherin (Kap)/importin family.

221 nd H2B is mediated by several members of the karyopherin (Kap; importin) family.

222 The yeast karyopherin Kap114p has previously been shown to import

223 f the H2A and H2B NLSs specifically with the karyopherin Kap114p.

224 , deletion of the previously uncharacterized karyopherin KAP120 caused accumulation of Rpl11b-GFP in

225 w that Nup116 mediates nuclear import of the karyopherin Kap121, and each protein is required for mit

226 s of phenotypes associated with mutations in karyopherin Kap121p.

227 t Spo12p is imported into the nucleus by the karyopherin Kap121p.

228 import cargoes for the essential yeast beta-karyopherin, Kap121p.

229 sembly factor 1 (CAF-1), as well as upon the karyopherin Kap123p, but was independent of Cac2p, anoth

230 published results our data indicate that the karyopherin Kap142p is able to mediate nuclear import of

231 is impaired in nup82-3 and in mutants of the karyopherin KAP95, but is not affected by the loss of MS

232 yeast extracts by affinity chromatography on karyopherin Kap95p-coated beads.

233 (cNLS)-mediated nuclear import supported by karyopherins (Kaps) alpha and beta1.

234 Nucleocytoplasmic transport is sustained by karyopherins (Kaps) and a Ran guanosine triphosphate (Ra

235 Karyopherins (Kaps) transport cargo across the nuclear p

236 alent interactions with transport receptors (Karyopherins (Kaps)) that orchestrate nucleocytoplasmic

237 The alpha- and beta-karyopherins (Kaps), also called importins, mediate the

238 nuclear transport receptors (NTRs), such as karyopherins (Kaps), that mediate the trafficking of nuc

239 and is mediated by soluble carriers known as karyopherins (Kaps), transportins, importins, or exporti

240 localization signals that are recognized by karyopherins (Kaps).

241 e we show that Yap1p is a target of the beta-karyopherin-like nuclear exporter, Crm1p.

242 y regulates the subcellular distributions of karyopherins likely due to alteration of the RanGTP grad

243 isplacement of the C-terminal tail of Ran by karyopherins may be a general mechanism to facilitate Ra

244 The importin-beta family members (karyopherins) mediate the majority of nucleocytoplasmic

245 ansport of various substrates that use other karyopherin-mediated import or export pathways was not a

246 antagonizes interferon signaling by blocking karyopherin-mediated nuclear import processes.

247 d cellular state without the requirement for karyopherin-mediated nuclear import.

248 e proposed to function as stepping stones in karyopherin-mediated transport pathways.

249 ession of GSP1, the small GTPase that powers karyopherin-mediated transport, rescued mitochondrial an

250 ntify a novel link between E2 regulation and karyopherin-mediated transport.

251 f both NLS epitopes abolishes binding to the karyopherins, mislocalized NXF1 to the cytoplasm, and si

252 tic genetic interactions with mutants of the karyopherin MSN5.

253 We report here that the karyopherin Mtr10p is required for the normal accumulati

254 bic residues for activity, and show that the karyopherin Nmd5p is required for Crz1p nuclear import.

255 the main transport receptor in the importin/karyopherin nuclear import pathway.

256 us are recognized by members of the importin-karyopherin nuclear transport receptor family.

257 s demonstrate a global role for Gsp1p-GTP on karyopherin-nucleoporin interactions and provide a rudim

258 s light on the importance of finely adjusted karyopherin-nucleoporin interactions for efficient cargo

259 of the nuclear transport machinery including karyopherins, nucleoporins, and the Ran guanine-nucleoti

260 CC3 binds directly to the karyopherins of the importin beta family in a RanGTP-ins

261 Soluble karyopherins of the importin-beta (impbeta) family use R

262 cerevisiae gene YDR395w/SXM1 as a potential karyopherin on the basis of limited sequence similarity

263 ore machinery, similar to importin-beta/beta-karyopherin or other importin-beta-like import factors,

264 the nucleus by multiple transport receptors (karyopherins or importins).

265 at are recognized by import receptors termed karyopherins or importins.

266 NLSs) and cognate transport factors known as karyopherins or importins.

267 We find that most nucleoporins and karyopherins preferentially associate with a subset of h

268 a model where cytosolic histones bind import karyopherins prior to acetylation.

269 s underlies how transport receptors known as karyopherins proceed through a tethered layer of intrins

270 in B inhibits nuclear export mediated by the karyopherin protein chromosomal region maintenance 1 (CR

271 st there are at least 14 members of the beta-karyopherin protein family that govern the movement of a

272 also known as KAP114, is one of 14 importin/karyopherin proteins in yeast.

273 this analysis we have identified three other karyopherins, Pse1p/Kap121p, Sxm1p/Kap108p, and Nmd5p/Ka

274 Consistent with their ability to inhibit the karyopherin-PY-STAT1 interaction, Zaire, mouse-adapted Z

275 well as a rationale for interactions of the karyopherin-Ran complex with the regulatory proteins ran

276 Since each type of karyopherin receptor prefers particular Nups or uses a l

277 hree putative NLSs potentially recognized by karyopherin receptors is involved in nuclear localizatio

278 The understanding of how different karyopherins recognize human NXF1, the examination of NX

279 ved family of soluble transport factors, the karyopherins (referred to as importins and exportins).

280 exportin complex Cse1p.Gsp1p.GTP function as karyopherin release factors (KaRFs) because they can acc

281 p1p, Nup2p, Cse1p, and Gsp1p may function as karyopherin release factors (or KaRFs) in the nuclear ba

282 ctation, we show here that cells lacking the karyopherin required for Hog1 nuclear import or in which

283 It has been shown that chaperones or karyopherins responsible for import can maintain the sta

284 interactions of NXF1 homologues with various karyopherins reveals the evolutionary development of red

285 slocation of HDAC3 via the phoshorylation of karyopherin subunit alpha2 and alpha6.

286 Proteins of the karyopherin superfamily including importins and exportin

287 provide a rudimentary map of the routes that karyopherins take as they cross the nuclear pore complex

288 Kap123p is a yeast beta-karyopherin that imports ribosomal proteins into the nuc

289 Indeed, alpha-karyopherins that mediate nuclear import bound to alphaK

290 members of a family of transport receptors (karyopherins) that mediate the nucleocytoplasmic transpo

291 The soluble transport factor, or karyopherin, that mediates the import of Lhp1p is Kap108

292 proteins (Rab6 and Vps53) in viral entry, a karyopherin (TNPO3) in viral integration, and the Mediat

293 ied proteins by mass spectrometry, and found karyopherins to be one of the major groups of proteins e

294 tion may be a general mechanism used by beta-karyopherins to recognize transport substrates.

295 al proteins and transport them together with karyopherins to their nuclear destination.

296 ons of Nup116p and Nup100p directly bind the karyopherin transport factor Kap95p during nuclear prote

297 The karyopherin transportin SR2 (TRN-SR2, TNPO3) is responsi

298 We now find that the distantly related karyopherin, transportin, negatively regulates nuclear e

299 iation of Ran x GTP with transport carriers (karyopherins) triggers the loading/unloading of export o

300 In addition to the karyopherins, we identified Rai1p, a protein previously

301 p153, are recognized by transport receptors (karyopherins) when trafficking large molecular cargos th