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1 eta's binding sites both for Ran-GTP and for karyopherin alpha.
2 ing is also involved in the association with karyopherin alpha.
3 nt of the cellular nuclear import machinery, karyopherin alpha.
4 S cargo that is imported into the nucleus by karyopherin alpha.
5 b2p NLS substrate in vitro in the absence of karyopherin alpha.
6  inhibited import, did not inhibit export of karyopherin alpha.
7 rt assays, we have mapped binding regions of karyopherin alpha.
8 x and is modulated through interactions with karyopherin alpha.
9  beta to karyopherin alpha covers the NLS of karyopherin alpha.
10 ich together comprise the NPI-1 subfamily of karyopherin alphaS.
11 own assays indicate interaction of APE1 with karyopherin alpha 1 and 2, which requires the 20 N-termi
12 uclear import complex formation by tethering karyopherin alpha 2 and karyopherin beta 1 to the membra
13 t mutations in the C terminus no longer bind karyopherin alpha 2 or block the nuclear import of STAT1
14    We also show that N-terminal deletions of karyopherin alpha 2 that no longer bind to karyopherin b
15 mbinant SARS-CoV lacking ORF6 did not tether karyopherin alpha 2 to the ER/Golgi membrane and allowed
16    We mapped the region of ORF6, which binds karyopherin alpha 2, to the C terminus of ORF6 and show
17 ns that interact with the NLS and identified karyopherin alpha 3 (KPNA3 or Kap-alpha3) and karyopheri
18 e preferential interaction of W protein with karyopherin-alpha 3 and karyopherin-alpha 4.
19 aryopherin alpha 3 (KPNA3 or Kap-alpha3) and karyopherin alpha 4 (KPNA4 or Kap-alpha4) as key binding
20 on of W protein with karyopherin-alpha 3 and karyopherin-alpha 4.
21  solution binding assay, Vpr associated with karyopherin alpha, a cellular receptor for NLSs.
22 egulatory role in this process by binding to karyopherin alpha, a cellular receptor for nuclear local
23 ions as a molecular bridge between VirE2 and karyopherin alpha, allowing VirE2 to utilize the host ce
24  (pendulin/Rch1/alpha-P1/hSrp1alpha and Srp1/karyopherin-alpha/alpha-S1/NPI-1) which function in nucl
25                                Surprisingly, karyopherin alpha also contains an NLS.
26 g as an 'adapter' molecule between VirE2 and karyopherin alpha and 'piggy-backing' VirE2 into the hos
27                               Interaction of karyopherin alpha and a classical nuclear localization s
28 heterodimeric receptor comprised of importin/karyopherin alpha and beta.
29 dimeric import receptor composed of importin/karyopherin alpha and beta.
30 y to the overlapping site, Ran-GTP displaces karyopherin alpha and binds to karyopherin beta.
31 ng region for Ran-GTP overlaps with that for karyopherin alpha and comprises at least one of the two
32 karyopherin beta1/importin beta, which binds karyopherin alpha and mediates the nuclear import of the
33 gions of karyopherin beta that interact with karyopherin alpha and with Ran-GTP.
34 nsportation of DP rcDNA via interaction with karyopherin-alpha and -beta.
35 ed with cellular nuclear transport receptors karyopherin-alpha and -beta.
36 hese sequences can mediate direct binding to karyopherin-alpha and are essential for the passage of i
37  cargoes and their import receptor proteins, karyopherin-alpha and karyopherin-beta, can be robustly
38   Using truncated forms of recombinant yeast karyopherins alpha and beta in in vitro binding assays,
39 n shown to act as general transport factors (karyopherin alpha) and nuclear pore-docking proteins to
40 p is transported into the nucleus by a Ran-, karyopherin alpha- and beta-dependent mechanism.
41 red by indirect immunofluorescence with anti-karyopherin alpha antibodies, we found that karyopherin
42                                        Thus, karyopherin alpha belongs to a group of proteins that co
43 t T cells to target members of the importin (karyopherin)-alpha beta NLS receptor complex.
44  which have been termed importin alpha/beta, karyopherin alpha/beta, or PTAC 58/ 97.
45 ion sequence-bearing proteins is mediated by karyopherin alpha/beta1 heterodimers.
46 binding results, karyopherin beta2 inhibited karyopherin alpha/beta1-mediated import of a classical N
47          Moreover, we found that Ran-GTP and karyopherin alpha bind to overlapping sites on karyopher
48  one of the two acidic clusters required for karyopherin alpha binding in addition to further downstr
49 her downstream determinants not required for karyopherin alpha binding.
50        Hence, binding of karyopherin beta to karyopherin alpha covers the NLS of karyopherin alpha.
51 l and then imported into its nucleus via the karyopherin alpha-dependent pathway.
52 -karyopherin alpha antibodies, we found that karyopherin alpha export was stimulated by added GTPase
53 nteraction with all six members of the human karyopherin alpha family.
54 ion signal-binding proteins belonging to the karyopherin alpha family.
55 model in which Rch1 or another member of the karyopherin-alpha family, through the recognition of the
56 zation signal (NLS) by Rch1, a member of the karyopherin-alpha family.
57                                 We find that karyopherin alpha first releases RanGTP from karyopherin
58   This association increased the affinity of karyopherin alpha for basic-type NLSs, including that of
59 erin alpha overlaps with the binding site of karyopherin alpha for karyopherin beta.
60 nal significance of specific residues within karyopherin alpha for NLS cargo binding.
61  assay in which export of endogenous nuclear karyopherin alpha from nuclei of digitonin-permeabilized
62 monstration that a glutathione S-transferase-karyopherin alpha fusion interacts with ORF29p, but not
63                                       Hence, karyopherin alpha import into and export from nuclei are
64 ar localization signal (NLS) is dependent on karyopherin alpha/importin alpha, which acts as the NLS
65 ure of a 50 kDa fragment of the 60 kDa yeast karyopherin alpha, in the absence and presence of a mono
66 factor Srp1 (also known as importin alpha or karyopherin alpha) is required for ubiquitin-independent
67 lves of the ring are structurally related to karyopherin-alpha (Kap-alpha) and beta-karyopherin famil
68                                 Furthermore, karyopherin-alpha, karyopherin-beta1 and the Ran GTPase
69 IFN) signaling by binding to NPI-1 subfamily karyopherin alpha (KPNA) nuclear import proteins, preven
70  its interaction with the NPI-1 subfamily of karyopherin alpha (KPNA) nuclear transporters.
71 AT1 (PY-STAT1), which occurs via a subset of karyopherin alpha (KPNA) nuclear transporters.
72 of Ebola virus (EBOV) VP24 protein with host karyopherin alpha (KPNA) proteins blocks type I interfer
73                      We analyzed the role of karyopherin alpha (KPNA), a key classical nuclear import
74 beta2 or transportin, and does not require a karyopherin alpha-like adapter protein.
75                  We have also expressed each karyopherin alpha mutant in vivo as the only cellular co
76 of hRch1/hSrp1alpha/importin-alpha and hSrp1/karyopherin alpha/NPI-1, respectively, and show consider
77 ed in sequestration of most of the cytosolic karyopherin alpha or karyopherin beta, respectively, in
78                        Recently, a number of karyopherins alpha, or NLS-binding proteins, have been i
79                                   The NLS of karyopherin alpha overlaps with the binding site of kary
80                     We established that five karyopherin alpha paralogs are expressed by primary mous
81 cled efficiently in a reaction that involves karyopherin alpha, RanBP1, RanGAP, and the C terminus of
82                                              Karyopherin alpha recognizes "classical" monopartite and
83       We found that the C-terminal region of karyopherin alpha recognizes the nuclear localization se
84 ta's binding region for alpha and Ran-GTP or karyopherin alpha's binding region for beta resulted in
85                                              Karyopherin alpha's binding region for karyopherin beta
86 gous to those previously shown to constitute karyopherin alpha's binding site to karyopherin beta.
87 ng sites/regions in Saccharomyces cerevisiae karyopherin alpha (SRP1).
88                          We found that yeast karyopherin alpha/Srp1p and Crm1p are required for the n
89 phorylated and imported into the nucleus via karyopherin alpha/Srp1p.
90 a cytoplasmic receptor such as the importin (karyopherin)-alpha subunit.
91 tro binding assays, we mapped the regions of karyopherin alpha that bind to karyopherin beta and the
92 of matrix antigen and prevent its binding to karyopherin alpha, the cellular receptor for nuclear loc
93 ransferase pull-down assays, TDP-43 bound to karyopherin-alphas, thereby confirming the classical nuc
94 explains the observed cooperative binding of karyopherin alpha to a heterologous NLS protein in the p
95  the Bag6 nuclear localization sequence from karyopherin alpha to retain Bag6 in the cytosol but also
96   There was a correlation between binding of karyopherins alpha to different NLSs and their ability t
97                                            A karyopherin alpha variant with a mutation in the major N
98                 However, we also find that a karyopherin alpha variant with a mutation in the minor N
99                 In addition to binding plant karyopherin alpha, VirE3 interacts with VirE2, a major b
100                    RanGTP-mediated export of karyopherin alpha was inhibited by peptides representing
101 hereas karyopherin beta's binding region for karyopherin alpha was localized to an internal region co
102 ed to the creation of conditional alleles of karyopherin alpha with well characterized defects in NLS

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