ライフサイエンスコーパス: karyopherin beta

1 n via a classical import mechanism utilizing karyopherin beta.

2 diated hydrolysis of GTP on Ran complexed to karyopherin beta.

3 ding was not competed out by the addition of karyopherin beta.

4 eterodimer and, in doing so, associated with karyopherin beta.

5 uanosine diphosphate (GDP)-bound form and to karyopherin beta.

6 th the binding site of karyopherin alpha for karyopherin beta.

7 Ran-GDP bound only weakly to karyopherin beta.

8 GTP displaces karyopherin alpha and binds to karyopherin beta.

9 nstitute karyopherin alpha's binding site to karyopherin beta.

10 heterologous NLS protein in the presence of karyopherin beta.

11 ryopherin alpha bind to overlapping sites on karyopherin beta.

12 e (NLS), whereas its N-terminal region binds karyopherin beta.

13 f karyopherin alpha 2 that no longer bind to karyopherin beta 1 still retain ORF6 binding activity bu

14 rmation by tethering karyopherin alpha 2 and karyopherin beta 1 to the membrane.

15 nding protein 2 (RanBP2, a nucleoporin), and karyopherin beta (a component of the nuclear protein imp

16 RanBP1 also promotes the binding of Ran to karyopherin beta (also called importin beta and p97) and

17 Transport factors in the karyopherin-beta (also called importin-beta) family medi

18 karyopherin alphabeta stimulate recycling of karyopherin beta and Ran in a manner similar to the C te

19 hat stoichiometric complex formation between karyopherin beta and Ran-GTP renders Ran-GTP inaccessibl

20 nalysis of the effects on RanGAP activity by karyopherin beta and RanBP1 revealed a combination of co

21 d exchange assays, with yeast proteins, that karyopherin beta and RanGTP are recycled efficiently in

22 he regions of karyopherin alpha that bind to karyopherin beta and the regions of karyopherin beta tha

23 s, the docking of Vpr+ PICs was dependent on karyopherin beta and was blocked by antibodies to beta.

24 proteins expressed in Escherichia coli, that karyopherin beta binds to Ran-GTP and inhibits GTP hydro

25 ort receptor proteins, karyopherin-alpha and karyopherin-beta, can be robustly measured and that quan

26 As with the yeast protein, mammalian karyopherin beta completely blocked RanGAP activity.

27 function requires dissociation of the RanGTP-karyopherin beta complex.

28 f full-length yeast importin-beta (Kap95p or karyopherin-beta) complexed with RanGTP, which provides

29 rolysis by karyopherin beta was dependent on karyopherin beta concentration relative to Ran-GTP.

30 omplete inhibition of RanGAP was observed at karyopherin beta concentrations that were equimolar to R

31 tor Pax6 is imported into the nucleus by the Karyopherin beta family member Karyopherin 13 (Kap13).

32 structural basis for the specificity of the karyopherin-beta family for the GTP-bound state of Ran,

33 at renders RanGTP inaccessible to RanGAP and karyopherin beta functionally inactive.

34 ptor complex comprised of the Rch1/importin (karyopherin)-beta heterodimer expressed in Jurkat T cell

35 karyopherin alpha first releases RanGTP from karyopherin beta in a reaction that does not require GTP

36 ability of RanBP1 to associate with Ran and karyopherin beta in a ternary complex, and RanBP1 bindin

37 To further define the roles of RanBP1 and karyopherin beta in Ran function, we explored the effect

38 ith karyopherins; each bound 6--10 different karyopherin betas, including importins as well as export

39 Yeast karyopherin beta inhibits the GTP hydrolysis by Ran cata

40 These results demonstrate that RanBP1 and karyopherin beta interact with distinct sites of Ran and

41 human papillomavirus type 11 (HPV11) L2 with karyopherin beta (Kap beta) nuclear import receptors rev

42 ne pSTAT3 translocates to the nucleus by the karyopherin-beta nucleocytoplasmic system and binds DNA.

43 ction, we explored the effects of RanBP1 and karyopherin beta on mammalian proteins known to regulate

44 Like RanBP1, karyopherin beta prevented the release of GTP from Ran s

45 We found that the karyopherin beta-related export receptor CRM1 competes w

46 f most of the cytosolic karyopherin alpha or karyopherin beta, respectively, in complexes containing

47 madillo and Heat repeats of beta-catenin and karyopherin-beta, respectively.

48 sion in yeast of fragments containing either karyopherin beta's binding region for alpha and Ran-GTP

49 several clusters of basic residues, whereas karyopherin beta's binding region for karyopherin alpha

50 Karyopherin beta's binding region for Ran-GTP overlaps w

51 We discovered that karyopherin beta's binding site for Ran-GTP shows a stri

52 Here we report the mapping of karyopherin beta's binding sites both for Ran-GTP and fo

53 p and not with other members of the importin/karyopherin beta superfamily.

54 y soluble nuclear transport receptors of the karyopherin-beta superfamily termed importins and export

55 bind to karyopherin beta and the regions of karyopherin beta that interact with karyopherin alpha an

56 showed that RanGTP forms a 1:1 complex with karyopherin beta that renders RanGTP inaccessible to Ran

57 ighly specific inhibitor of CRM1, a cellular karyopherin-beta that transports nuclear export signal-c

58 We also mapped Ran's binding site for karyopherin beta to a cluster of basic residues analogou

59 filtration experiments, we found Ran-GTP and karyopherin beta to form a stoichiometric complex.

60 Hence, binding of karyopherin beta to karyopherin alpha covers the NLS of

61 nterfering RNA screens previously identified karyopherin beta transportin-3 (TNPO3) and NPC component

62 ition of RanGAP-stimulated GTP hydrolysis by karyopherin beta was dependent on karyopherin beta conce

63 Karyopherin alpha's binding region for karyopherin beta was localized to its N-terminal domain,