ライフサイエンスコーパス: katanin

1 ubunits of the microtubule-severing complex, katanin.

2 ule severing enzymes, Spastin, Fidgetin, and Katanin.

3 axon lose their characteristic resistance to katanin.

4 related to the microtubule-severing protein katanin.

5 in vitro; thus, it is an ortholog of animal katanin.

6 centrosome-associated WD repeat protein p80-katanin.

7 y subunit of the microtubule-severing enzyme Katanin.

8 subunits of the microtubule-severing ATPase katanin.

9 altered with regard to their sensitivity to katanin.

10 5, and the microtubule-severing protein, p60/katanin.

11 which results in ubiquitin laddering of p60/katanin.

12 that regulate the severing properties of P60-katanin.

13 interacts with ciliary proteins and p60/p80 katanins.

14 Shotgun) and the microtubule-linked proteins Katanin-60, EB1, Milton and aPKC.

15 The microtubule-severing protein katanin, a heterodimer of 60 and 80 kDa subunits, was pr

16 as been observed in vitro to be catalyzed by katanin, a heterodimeric adenosine triphosphatase that c

17 Katanin, a member of the AAA adenosine triphosphatase (A

18 equired after meiosis to negatively regulate katanin, a microtubule-severing complex, permitting the

19 Katanin action is required both for normal alignment and

20 Here, we demonstrate that katanin activity depends upon the behavior of the microt

21 plant cell wall since mutants with decreased katanin activity have been shown to have defective walls

22 zymes and suggest a scheme for regulation of katanin activity in cells dependent on free tubulin conc

23 We show that katanin activity is essential.

24 d in part by its absolute levels, given that katanin activity is high during mitosis.

25 any biological function, serve as sites for katanin activity.

26 Models that assumed that katanin acts on a uniform microtubule lattice were incom

27 itro data, whereas a model that assumed that katanin acts preferentially on spatially infrequent micr

28 p60 katanin, an AAA protein that severs and depolymerizes mi

29 function of spastin, as well as potentially katanin and fidgetin, are highly coordinated.

30 characterized family members, Vps4, spastin, katanin and fidgetin.

31 Compound 5a directly targeted katanin and regulated the severing activity of katanin,

32 Two related enzymes, katanin and spastin, use the energy from ATP hydrolysis

33 urons with bFGF heightens expression of both katanin and spastin, which are proteins that sever micro

34 nt microtubule-severing proteins, namely P60-katanin and spastin.

35 lating the microtubule response to stress in katanin and spiral2 mutant made sepal shape dependent on

36 d this method to investigate the function of KATANIN and WRINKLED1 in cotton plant development.

37 KATNB1 with KATNA1, the catalytic subunit of Katanin, and other microtubule-associated proteins.

38 reviously identified MT destabilizers (Op18, katanin, and XKCM1/KinI).

39 protein that is recognized by an anti-human katanin antibody and that this protein is localized, at

40 s of neurons that had been injected with the katanin antibody compared with controls.

41 mplexes is significantly blocked by the anti-katanin antibody.

42 In contrast, Katanin appears to function primarily on anaphase chromo

43 tions in hereditary spastic paraplegias, and katanin are related microtubule-severing AAA ATPases inv

44 Spastin and P60-katanin are two distinct microtubule-severing proteins.

45 e identified the microtubule-severing enzyme katanin as a central player in controlling the organizat

46 table protection of the microtubules against katanin-based loss.

47 ules through both a spastin-based mode and a katanin-based mode.

48 vide correspondingly less protection against katanin-based severing.

49 New evidence suggests that katanin - best known for severing microtubules in their

50 ntify the effect of katanin concentration on katanin binding and severing activity.

51 nhibit severing activity by interfering with katanin binding to microtubules.

52 The mechanism by which spastin and katanin break and destabilize microtubules is unknown, i

53 nal growth is sensitive to the levels of P60-katanin, but that other factors contribute to modulating

54 We also find that katanin can remove tubulin dimers from the ends of MTs,

55 tion mutations in the Caenorhabditis elegans katanin catalytic subunit, MEI-1, cause specific defects

56 e characterized the N-terminal domain of the katanin catalytic subunit.

57 s the noncatalytic regulatory p80 subunit of katanin, cause severe microlissencephaly.

58 MEI-1 may ensure temporal activation of the katanin complex during meiosis, whereas CRL3(MEL-26)-med

59 We quantify the effect of katanin concentration on katanin binding and severing ac

60 final levels of severing, and sensitivity to katanin concentration over the range 6-300 nM.

61 We find that severing activity depends on katanin concentration.

62 Expression of a dominant-negative P60-katanin construct in cultured neurons inhibits microtubu

63 Furthermore, katanin deficiencies phenocopy a mutation of beta-tubuli

64 We found that katanin-dependent MT severing was increased in X. tropic

65 increasing microtubule density and a second, katanin-dependent phase that occurs after microtubule de

66 We show that D-spastin, like katanin, displays ATPase activity and uses energy from A

67 n of branches, whereas overexpression of P60-katanin does not.

68 Consistent with this finding, GFP-tagged katanin driven by its native promoter localizes at sites

69 ediate the spindle-pole assembly activity of katanin during female meiosis.

70 a mutation in the p80 regulatory subunit of katanin, encoded by the PF15 gene in Chlamydomonas, alte

71 Cotton plants with suppressed KATANIN expression produced shorter fibers and elevated

72 mology domain protein encoded by aspm-1, the katanin family member mei-1, and the kinesin-12 family m

73 ase and Katanin p60-like 1 (Kat-60L1) of the Katanin family of microtubule severing proteins are requ

74 t of an E3 ubiquitin ligase complex, targets katanin for degradation during the transition from meios

75 Oligomerization increased the affinity of katanin for microtubules and stimulated its ATPase activ

76 Overexpression of katanin for short periods of time produced breaks prefer

77 How katanin fulfills its controlling role, however, remains

78 tubule-severing proteins, p56, EF1alpha, and katanin, has only confused the issue because none of the

79 to encode a protein with high similarity to katanin (hence FRA2 was renamed AtKTN1), a protein shown

80 In Caenorhabditis elegans, the katanin homologue MEI-1 is required for meiosis, but mus

81 on with antibodies specific for a vertebrate katanin homologue to demonstrate that katanin is respons

82 roblasts to be more resistant to severing by katanin in a manner that was not dependent on the acetyl

83 and activity of the MT-severing protein p60-katanin in interneurons to promote the rapid remodeling

84 -1 and its activator PPFR-1 ensure efficient katanin inactivation in the transition to mitosis.

85 was degraded after meiosis, contributing to katanin inactivation.

86 spindle shortening proceeds through an early katanin-independent phase marked by increasing microtubu

87 We found that purified katanin induced an ATP-dependent severing of the Chlamyd

88 Katanin inhibition lengthened spindles in both species.

89 Here we show that p60/katanin interacts with a complex consisting of Cul3 and

90 Katanin is a conserved AAA ATPase with the ability to se

91 Katanin is a heterodimeric enzyme that severs microtubul

92 Katanin is a heterodimeric microtubule-severing protein

93 Katanin is a microtubule-severing complex whose catalyti

94 Katanin is a microtubule-severing enzyme that is concent

95 To determine whether katanin is also required for spindle maintenance, we mon

96 Katanin is an evolutionarily conserved microtubule (MT)-

97 Katanin is broadly distributed in the neuron, and theref

98 mmunofluorescence analysis demonstrated that katanin is concentrated at a microtubule-dependent struc

99 The microtubule-severing enzyme katanin is essential for plants to form aligned microtub

100 esults indicate that microtubule-severing by katanin is essential for releasing microtubules from the

101 rotubules at the centrosome, indicating that katanin is indeed required for microtubule release from

102 P60-katanin is more highly expressed in the neuron, but spas

103 When katanin is overexpressed in fibroblasts, the microtubule

104 es on cultured sympathetic neurons show that katanin is present at the centrosome, but is also widely

105 that sensitivity to microtubule severing by katanin is regulated by a balance of factors, including

106 e sensitivity of microtubules to severing by katanin is regulated by acetylation of the microtubules.

107 This suggests that katanin is regulated in part by its absolute levels, giv

108 Previous work showed that katanin is required for severing at points where two mic

109 This result suggests that katanin is responsible for changes in microtubules occur

110 ebrate katanin homologue to demonstrate that katanin is responsible for the majority of M-phase sever

111 We conclude that katanin is solely responsible for severing at CMT crosso

112 Similarly, in neuronal cultures, katanin levels are high when axons are allowed to grow a

113 Using various rat tissues, we found that P60-katanin levels are much higher than spastin levels durin

114 In the adult, P60-katanin levels plunge dramatically but spastin levels de

115 rodent brain, neurons vary significantly in katanin levels, depending on their developmental stage.

116 HDC5-deficient cells show an increase in p60/katanin levels, indicating that Cul3/Ctb9/KLHDC5 is requ

117 Taken together, these data suggest that a katanin-like mechanism may mediate the severing of the o

118 These results indicate that the katanin-like protein is essential for oriented cellulose

119 We further demonstrated that the Arabidopsis katanin-like protein possessed MT-severing activity in v

120 gether, these results suggest that AtKTN1, a katanin-like protein, is essential not only for normal c

121 responsible for the fra2 mutation encodes a katanin-like protein.

122 Strikingly, katanin localizes and severs at the interface of GMPCPP-

123 found in adult mouse brain, indicating that katanin may have other functions distinct from its mitot

124 crotubules for a fixed pool of tubulin, with katanin-mediated severing allowing easier access to this

125 loped to enable the real-time observation of katanin-mediated severing of individual, mechanically un

126 ed that phototropin photoreceptors stimulate katanin-mediated severing specifically at microtubule in

127 er the microtubule-severing protein known as katanin mediates microtubule release from the neuronal c

128 n complex is the microtubule-severing enzyme katanin (MEI-1).

129 In Caenorhabditis elegans, the MEI-1-katanin microtubule-severing complex is required for mei

130 microtubules and then dissociated into free katanin monomers.

131 eric protein phosphatase 4 complex, enhanced katanin MT-severing activity during C. elegans meiosis.

132 owed that CMTs fail to become ordered in the katanin mutant.

133 was completely abolished in the Arabidopsis katanin mutant.

134 titative imaging experiments and analysis of katanin mutants showed that the longitudinal arrays are

135 r assay demonstrated that the p60 subunit of katanin oligomerized in an adenosine triphosphate (ATP)-

136 After hydrolysis of ATP, microtubule-bound katanin oligomers disassembled microtubules and then dis

137 The net effect of katanin on the polymer mass depends on the microtubule t

138 Depletion of either katanin or spastin with siRNA diminished but did not eli

139 required for transport) pathway and spastin, katanin p60 and fidgetin affecting multiple aspects of c

140 ks an inhibitory phosphorylation site in the katanin p60 catalytic subunit.

141 nown regarding the role of the family member Katanin p60 subunit A-like 1, KATNAL1, in central nervou

142 IS1 homology (LisH) motif, including several katanin p60 subunits, muskelin, tonneau, LEUNIG, Nopp140

143 Katanin p60 was the first ATPase associated with microtu

144 irement for the microtubule-severing protein katanin p60-like 1 (Kat-60L1) in regulating the elaborat

145 Here, we show that both the Ik2 kinase and Katanin p60-like 1 (Kat-60L1) of the Katanin family of m

146 rogression through ubiquitination of phospho-katanin p60.

147 In this work, we investigate how katanin (p60), believed to be the first discovered sever

148 red that microtubule severing by the protein katanin plays a crucial and unexpected role in the reori

149 We propose that katanin preferentially severs older, post-translationall

150 bules renders them notably less sensitive to katanin, prompting us to posit that microtubule disinteg

151 ERH3 encodes a p60 katanin protein that is expressed throughout the plant.

152 These results indicated that katanin protein, but not katanin's microtubule-severing

153 Katanin proteins are known to sever microtubules, and ha

154 881) show that microtubule severing by katanin provides a means for increasing microtubule dens

155 We also show that katanin reduces the levels of several types of post-tran

156 Although katanin reduces the polymer mass and destabilizes the in

157 The katanin regulatory subunit in turn caused a dramatic cha

158 necessary and sufficient for binding to the katanin regulatory subunit.

159 rt a model in which MBK-2 down-regulates the katanin-related protein MEI-1 to control spindle positio

160 l3/Ctb9/KLHDC5 is required for efficient p60/katanin removal.

161 croinjection of an antibody that inactivates katanin results in a dramatic accumulation of microtubul

162 Overexpression of wild-type P60-katanin results in excess microtubule severing and is al

163 f short microtubules, whereas the excess P60-katanin results in short microtubules intermingled with

164 ant MT orientation caused by the mutation of katanin results in the distorted deposition of cellulose

165 These results indicate katanin's activities are segregated into a subunit (p60)

166 To determine the relationship between katanin's microtubule-severing activity and its role in

167 ults indicated that katanin protein, but not katanin's microtubule-severing activity, is required for

168 Here, we investigate the activity of katanin severing using a GFP-labeled human version.

169 It may be that katanin severs microtubules throughout the cell body to

170 We show that inhibition of katanin slows the rate of spindle shortening in nocodazo

171 udy using electron tomography has found that katanin stimulates the production of microtubules in the

172 veral oocyte proteins, including the meiotic katanin subunit MEI-1 and the oocyte maturation protein

173 In mbk-2 mutants, the meiosis-specific katanin subunits MEI-1 and MEI-2 persist during mitosis

174 e report the sequences and activities of the katanin subunits.

175 rongly shield them from being severed by P60-katanin than by spastin.

176 n the axon are more resistant to severing by katanin than microtubules elsewhere in the neuron.

177 nstrate a novel regulatory mechanism for p60/katanin that occurs at the level of targeted proteolysis

178 In dividing cells, the levels of P60-katanin (the subunit with severing properties) increase

179 e severing protein that shares homology with katanin, the microtubule severing activity of which prom

180 Katanin, the microtubule-severing protein, consists of a

181 s of the microtubule-severing protein termed katanin to completely break down the axonal microtubule

182 the sufficient and necessary conditions for katanin to promote array alignment, stresses the critica

183 mportant because tau regulates the access of katanin to the microtubule.

184 onal microtubules against excess severing by katanin, under conditions of tau depletion.

185 The microtubule-severing enzyme katanin uses ATP hydrolysis to disrupt noncovalent bonds

186 Surprisingly, katanin was also found in adult mouse brain, indicating

187 The microtubule-severing subunit p60 of katanin was identified as a candidate substrate for MAB1

188 nsitivity of the microtubules to severing by katanin was increased or decreased, respectively.

189 To understand the nonsevering activities of katanin, we characterized the N-terminal domain of the k

190 nd functional similarity between spastin and katanin, we hypothesized that spastin promotes the dynam

191 tanin and regulated the severing activity of katanin, which cut the cellular microtubules into short