ライフサイエンスコーパス: keratocyte

1 g motility and crescent morphology of a fish keratocyte.

2 lamellipodium of a migrating fish epidermal keratocyte.

3 o crucial to lamellipodia-based migration of keratocytes.

4 ively, 442% and 431%; P < 0.002) compared to keratocytes.

5 regulation in TGF-beta1- and FGF-2-activated keratocytes.

6 ir mRNAs were downregulated in the activated keratocytes.

7 ement oscillations in motile fish epithelial keratocytes.

8 TGF-beta1 concentrations than did posterior keratocytes.

9 the flat, fan-shaped lamellipodial domain of keratocytes.

10 has a significant role in the activation of keratocytes.

11 ession induced by TGFbeta1 in rabbit corneal keratocytes.

12 ated stroma containing isolated and enlarged keratocytes.

13 symmetry breaking and polarized motility of keratocytes.

14 role for CD18 in mediating PMN contact with keratocytes.

15 pellets of several genes highly expressed by keratocytes.

16 g and motility initiation in fish epithelial keratocytes.

17 g and motility initiation in fish epithelial keratocytes.

18 could be used to express exogenous genes in keratocytes.

19 ck of contamination with epithelial cells or keratocytes.

20 a membrane of rapidly moving fish epithelial keratocytes.

21 xhibit enhanced oxidative stress compared to keratocytes.

22 vectors to treat diseases affecting corneal keratocytes.

23 the cleavage occurred beyond the last row of keratocytes.

24 n myofibroblasts (857 genes), exclusively in keratocytes (409 genes), or in both phenotypes (252 gene

25 ecent studies have shown that rabbit corneal keratocytes abundantly express two water-soluble protein

26 nanostructures, in combination with a model keratocyte actin meshwork.

27 in opposite directions, when traversing the keratocyte actin network.

28 ors, modifies their movement trajectories on keratocyte actin networks.

29 We also observed that the RI drops after keratocyte activation (RI = 1.365 +/- 0.003), leading to

30 on was detected between the thickness of the keratocyte activation zone and the spheroequivalent refr

31 ences between anterior and posterior corneal keratocytes after stimulation with the profibrotic agent

32 Herein, we demonstrated that murine keratocytes also acquired a fibroblastic shape and lost

33 of MMC, especially with regard to decreased keratocyte and corneal endothelial cell populations note

34 rmation of, many proteins that may influence keratocyte and nerve growth cone behavior in the cornea.

35 orneal stroma involves close contact between keratocytes and collagen.

36 the paralimbus developed close contacts with keratocytes and collagen.

37 her cells with broad LP regions, namely fish keratocytes and Drosophila S2 cells.

38 ers expressed T4 receptor alpha (THRA) mRNA; keratocytes and endothelial cells expressed T4 receptor

39 IGF and PDGF BB stimulated elongation of keratocytes and extension of dendritic processes within

40 aracterized by a granular appearance of both keratocytes and extracellular matrix.

41 g on several different cell types, including keratocytes and fibroblasts, we discuss how dynamic cell

42 in intact tissue but was absent in cultured keratocytes and fibroblasts.

43 ful marker for the identification of corneal keratocytes and for documenting their response to enviro

44 s demonstrated that MT1-MMP was expressed in keratocytes and immortalized corneal fibroblast cell lin

45 To determine the role of keratocytes and leukocyte beta(2) (CD18) integrins in ne

46 utes total time), does not cause toxicity to keratocytes and may be used to stiffen corneas thinner t

47 The cell volume of calcein AM-loaded keratocytes and myofibroblasts was determined by using n

48 e NRF2-mediated antioxidant response in both keratocytes and myofibroblasts, and (3) modified the TGF

49 EphrinB1 was expressed in corneal-resident keratocytes and neutrophils.

50 tive levels of ECM components synthesized by keratocytes and secreted into the media were evaluated b

51 In protein-free media, both primary keratocytes and selected clonal cells aggregated to form

52 Embryonic corneal keratocytes and sensory nerve fibers grow and differenti

53 Serum-free cultured rabbit corneal keratocytes and TGFbeta (5 ng/mL) induced myofibroblasts

54 hing of refractive indices between quiescent keratocytes and the surrounding tissue, and that after s

55 tally measured values of the tension in fish keratocytes and their dependence on the number of adhesi

56 of corneal nerves, number of hyperreflective keratocytes, and corneal epithelial, endothelial, and ke

57 3 were each expressed by epithelium, stromal keratocytes, and infiltrated CD11b(+) cells in corneas.

58 keratocytes, increased presence of activated keratocytes, and inflammatory cells in the anterior stro

59 opodia, such as can be found in fibroblasts, keratocytes, and neuronal growth cones.

60 density of basal epithelial cells, anterior keratocytes, and posterior keratocytes did not show stat

61 density of basal epithelial cells, anterior keratocytes, and posterior keratocytes did not show stat

62 lantation were used to determine the role of keratocyte apoptosis in the failure of orthotopic alloge

63 Corneal keratocytes are able to differentiate normally and respo

64 Although keratocytes are normally characterized by the persistent

65 that, even at late embryonic stages, corneal keratocytes are not terminally differentiated, but maint

66 Quiescent keratocytes are thought to produce crystallins that matc

67 We developed embryonic zebrafish keratocytes as a model system for investigating establis

68 between corneal epithelial cells and corneal keratocytes as well as vascular endothelial cells.

69 NK2 levels via JNK1/2 DsiRNA transfection of keratocytes before their activation.

70 MSC have successfully differentiated into keratocytes both in vitro and in vivo, and corneal epith

71 pattern followed the distribution of stromal keratocytes but did not stain epithelium or endothelium.

72 y relation, which has been measured for fish keratocytes but remains unexplained.

73 The bonding treatment was not toxic to keratocytes but slightly reduced the migration of cornea

74 ressed in the corneal epithelium and stromal keratocytes, but was more abundant in the epithelium (74

75 cell was similar between myofibroblasts and keratocytes, but was substantially reduced as a percenta

76 ar enhancer of wound healing and fibrosis in keratocytes by augmenting a limited subset of the cellul

77 To this end, we challenged corneal stromal keratocytes by injecting them into a new environment alo

78 ins (ss-catenin and connexin 43), of stromal keratocytes (CD34), of apoptosis (terminal deoxynucleoti

79 es, and corneal epithelial, endothelial, and keratocyte cell densities were evaluated by in vivo slit

80 was also measured in a (donor-type) C57BL/6 keratocyte cell line after stimulation of Fas or via use

81 7 days in culture in all growth factors, but keratocyte cell morphology was only maintained in medium

82 Knowledge of keratocyte cell properties, function, and origin is limi

83 The results show that injected stromal keratocytes change their phenotype, proliferate and migr

84 ly recovered the ability to express a robust keratocyte-characteristic marker, CD34, while still expr

85 e results indicate that low-glucose enhances keratocyte-characteristic phenotype above and beyond est

86 re, low-glucose enhanced their reversal to a keratocyte-characteristic phenotype.

87 Although PMN-keratocyte contacts require CD18 integrins, contact with

88 eatment with TGFbeta1 and TGFbeta2 increased keratocyte contractility, as indicated by stress fiber f

89 hether myofibroblast differentiation altered keratocyte crystallin protein concentration and increase

90 transformed myofibroblasts expressing human keratocyte crystallins was measured by reflectance confo

91 Keratocytes cultured in fetal bovine serum also become f

92 Keratocytes cultured in IGF or PDGF BB maintain a quiesc

93 essed in normal rabbit corneal stroma and in keratocytes cultured in serum-free and insulin-supplemen

94 We previously showed primary keratocytes cultured under serum-free conditions to secr

95 Human keratocytes, cultured in a stable vitamin C derivative,

96 Using primary keratocyte cultures and various experimental approaches,

97 Mouse keratocyte cultures did not express 3G5.

98 Subconfluent human and bovine keratocyte cultures were 3G5 negative.

99 matrix, thus supporting the hypothesis that keratocyte cytoplasm does not scatter light in the norma

100 toxic to the cornea and entered into corneal keratocyte cytoplasm.

101 The mean anterior and posterior stromal keratocyte densities were significantly lower in the man

102 The anterior and posterior stromal keratocyte densities were statistically lower and stroma

103 a significant decrease in the mean anterior keratocyte density 1, 3, and 6 months postoperatively (P

104 al transparency is associated with decreased keratocyte density and quiescence and the expression of

105 A sustained reduction in keratocyte density does not affect vision or optical pro

106 Keratocyte density in the flap decreased by 20% during t

107 LASIK flap does not influence the changes in keratocyte density in the flap.

108 to be pronounced and long lasting as far as keratocyte density of the anterior and mid stroma is con

109 There was no significant change in posterior keratocyte density or endothelial density at any postope

110 The anterior mid-stromal keratocyte density showed a significant decrease (P < 0.

111 eoperative values, and the posterior stromal keratocyte density showed a significant increase at 1 an

112 6 months and 1, 3, and 5 years after LASIK, keratocyte density was measured using confocal microscop

113 Keratocyte density was measured with the Heidelberg Reti

114 in-situ keratomileusis demonstrated that the keratocyte density within the laser in-situ keratomileus

115 density, endothelial cell density, anterior keratocyte density, posterior keratocyte density, sub-ba

116 sity, anterior keratocyte density, posterior keratocyte density, sub-basal nerve density, sub-basal n

117 ll density; mean anterior, mid and posterior keratocyte density; qualitative analysis of stromal back

118 An R124H mutation in primary human corneal keratocytes derived from a GCD2 patient was corrected by

119 bryonic stem (hES) cells could elucidate the keratocyte developmental pathway and open a potential fo

120 l cells, anterior keratocytes, and posterior keratocytes did not show statistical significance betwee

121 l cells, anterior keratocytes, and posterior keratocytes did not show statistical significance betwee

122 ctor (TGF)-beta-dependence of feline corneal keratocyte differentiation into alpha-smooth muscle acti

123 nt cells produce different protrusions, from keratocytes dominated by lamellipodia, to growth cones c

124 PDGF induced keratocyte elongation without inducing stress fiber form

125 Gene expression profiles of the 12 samples (keratocytes +/- EP +/- TGF-beta1 for three preparations)

126 nts show that anterior and posterior corneal keratocytes exhibit different sensitivities to the profi

127 Keratocytes, expressing CD34 in normal human corneas, co

128 To test this model, we apply it to fish keratocytes, fast moving cells that maintain their morph

129 notopographic cues assist in stabilizing the keratocyte/fibroblast phenotype while pathologic microen

130 ng and initiation of actin-based motility in keratocytes (fish epithelial cells).

131 Keratocyte fragments [9, 10] are the simplest motile uni

132 We found that keratocyte fragments are the smallest motile electric fi

133 front and the rear of the cell are found in keratocyte fragments that lack a cell body.

134 We observed that, although keratocytes from 2 d postfertilization (dpf) embryos res

135 resembled canonical fan-shaped keratocytes, keratocytes from 4 dpf embryos often formed multiple pro

136 determine the protein expression pattern of keratocytes from different species and different culture

137 Derivation of keratocytes from human embryonic stem (hES) cells could

138 The keratocytes from three normal and three KC corneas were

139 tin polymerization, switches highly adherent keratocytes from waving to persistent protrusion.

140 and suggests a role for the ZEB1 protein in keratocyte function.

141 tocyte nuclei, suggesting a role for ZEB1 in keratocyte function.

142 folding-free pellet culture of hCSSC induces keratocyte gene expression patterns in these cells and s

143 Transcripts for these keratocyte genes -- FLJ30046, KERA, ALDH3A1, CXADR, PTGD

144 ically (SEM) and were used as substrates for keratocyte growth in vitro.

145 Motile cells - fan-like keratocytes, hand-shaped nerve growth cones, polygonal f

146 of glucose in the behaviour of human corneal keratocytes has been overlooked.

147 Corneal keratocytes have a remarkable ability to heal the cornea

148 orneal stromal cells (PLCSCs), which contain keratocytes, have a complex phenotype.

149 study was to determine whether human corneal keratocyte (HCKs) in culture synthesize these chemokines

150 xidative stress levels between human corneal keratocytes (HCKs), fibroblasts (HCFs) and keratoconus c

151 n corneal epithelial cells (HCECs) and human keratocytes (HKs) cultured on the optimal hybrid constru

152 enovirus type 2 (CAV-2) vectors to transduce keratocyte in vivo in mice and nonhuman primates, and ex

153 ium (P < .001) and higher density of stromal keratocytes in anterior and posterior stroma (P < .0001)

154 Corneal keratocytes in basal media within compressed matrices ha

155 ta gene transcription, can be used to expand keratocytes in culture without the use of AM in the futu

156 This separates along the last row of keratocytes in most cases performed with the BB techniqu

157 Cultured corneal fibroblasts and keratocytes in situ express RANKL, OPG, and M-CSF cytoki

158 Keratocytes in the corneal stroma express keratan sulfat

159 al nerve plexus and loss of anterior stromal keratocytes in the early postoperative period, with comp

160 The progressive loss of keratocytes in the flap and anterior portion of the resi

161 oxsackievirus adenovirus receptor-expressing keratocytes in the four species and, compared to mock-in

162 sis of stroma components indicated a loss of keratocytes in the upper stroma of keratoconic corneas a

163 rneal epithelial cells were found to fuse to keratocytes in vitro and to induce myofibroblast transfo

164 Apoptosis was induced in keratocytes in vitro by dual stimulation with agonistic

165 s of this study show that insulin can expand keratocytes in vitro, maintain their phenotype, and prev

166 S cells can be induced to differentiate into keratocytes in vitro.

167 und to be non-toxic to human corneal stromal keratocytes in vitro; however, they did induce productio

168 notypic change associated with activation of keratocytes in vivo and in vitro.

169 corin was retained in the cytoplasm of mouse keratocytes in vivo and of transfected human embryonic k

170 demonstrated cell-cell junctions typical of keratocytes in vivo.

171 thelial genes in the corneal endothelium and keratocytes, including the basement membrane component C

172 sity of the basal membrane; lower density of keratocytes, increased presence of activated keratocytes

173 the transition of quiescent corneal stromal keratocytes into contractile myofibroblasts.

174 g that attenuation of the differentiation of keratocytes into myofibroblast can significantly enhance

175 Keratocytes isolated from rabbit corneal stroma and plat

176 Keratocytes isolated from rabbit corneal stroma by colla

177 Keratocytes, isolated from rabbit corneal stroma, and cu

178 (dpf) embryos resembled canonical fan-shaped keratocytes, keratocytes from 4 dpf embryos often formed

179 ctly determine the polarity of the extracted keratocyte lamellipodium from the cell periphery to the

180 ferentiation of pluripotent hES cells to the keratocyte lineage.

181 On plastic dishes, human, bovine, and rabbit keratocytes lose their characteristic dendritic morpholo

182 Some crawling cells, such as fish keratocytes, maintain a roughly constant shape and veloc

183 The keratocyte markers aldehyde dehydrogenase and keratocan

184 a significant decrease in RNA levels for the keratocyte markers ALDH1A1, lumican, and keratocan and a

185 lls were cultured as substratum-free pellets keratocyte markers AQP1, B3GNT7, PTDGS, and ALDH3A1 were

186 yte proliferation and the maintenance of the keratocyte markers in 7-day cultures in cells plated at

187 Expression of myofibroblast and keratocyte markers was determined by real-time PCR and W

188 They proliferate and lose their keratocyte markers when they become fibroblastic during

189 ical feedbacks underlying wave generation in keratocytes may constitute a general module for establis

190 que extracellular matrix secreted by stromal keratocytes, mesenchymal cells of neural crest lineage.

191 platform for investigating the mechanics of keratocyte migration after exposure to specific wound-he

192 Both 10% FBS and PDGF stimulated significant keratocyte migration through the uncompressed outer matr

193 The keratocyte motif is caused by optimal recycling of the c

194 In fibroblasts and keratocytes, motility is actin dependent, while microtub

195 can inhibit TGFbeta1-induced rabbit corneal keratocyte-myofibroblast transformation.

196 Fundamentally, PMN migration along keratocyte networks constitutes the beginning of a new e

197 smission electron microscopy revealed intact keratocyte networks within the paralimbus that were morp

198 trastructural morphometric analysis of PMNs, keratocyte networks, and collagen.

199 ons from amoeboid cells into cells mimicking keratocytes, neurons, or fibroblasts.

200 Keratocytes normally express high levels of aldehyde deh

201 stoperatively, there was complete absence of keratocyte nuclei in 86% of corneas.

202 Staining of keratocyte nuclei with 4',6-diaminido-2-phenylindole dih

203 ZEB1 is present in keratocyte nuclei, suggesting a role for ZEB1 in keratoc

204 Scattered, presumed fragmented keratocyte nuclei, were observed at 1 and 3 months, but

205 stochemistry demonstrated ZEB1 expression in keratocyte nuclei.

206 and lumican expression in activated corneal keratocytes observed during corneal stromal wound healin

207 The results show that corneal keratocytes occupy a significantly greater tissue volume

208 e isolated from corneal epithelial cells and keratocytes of several species, including human (Hu), mo

209 Keratocytes of the corneal stroma produce a transparent

210 Keratocytes of the corneal stroma produce transparent ex

211 d of 5 to 11 collagen lamellae that revealed keratocytes on their anterior surface and in between.

212 sked which of the proteins that may regulate keratocytes or corneal nerve growth cone immigration int

213 ed by induction of neural cells, adipocytes, keratocytes, or TM cells.

214 We present a physical description of keratocyte oscillation in which periodic retraction of t

215 in the corneal stroma capable of assuming a keratocyte phenotype after extensive proliferation.

216 human corneal stromal stem cells to assume a keratocyte phenotype and to organize extracellular matri

217 The normal monkey keratocyte phenotype can be maintained in a low-calcium,

218 roma that maintain the potential to assume a keratocyte phenotype even after extensive replication.

219 The stability of the keratocyte phenotype was examined by switching KSFM to D

220 Keratocyte phenotype was induced by substratum-independe

221 ase 3A1, and keratocan, molecular markers of keratocyte phenotype.

222 Different rabbit keratocyte phenotypes also showed different levels of ex

223 ferent species and different cultured rabbit keratocyte phenotypes and to assess differences in light

224 ), and pig (P) and different cultured rabbit keratocyte phenotypes.

225 lings; however, they continued expression of keratocyte phenotypic markers throughout their replicati

226 e proteoglycans were degraded in cultures of keratocytes plated at low density and cultured in the ab

227 ver, JNK inhibition during the activation of keratocytes, pretreated with the JNK inhibitor, suppress

228 During wound-healing and in vitro, keratocytes proliferate, becoming fibroblastic, and lose

229 In KSFM, monkey keratocytes proliferated while maintaining the expressio

230 n, and platelet-derived growth factor-BB, on keratocyte proliferation and the maintenance of the kera

231 te coatings caused comparable enhancement of keratocyte proliferation compared with unmodified PMMA s

232 Keratocyte proliferation was measured by [3H]thymidine i

233 s also show abnormal corneal endothelial and keratocyte proliferation, corneal thickening, and corneo

234 In 1% FBS, AM-expanded keratocytes rapidly became alpha-SMA-expressing myofibro

235 Rabbit corneal keratocytes (RCKs) were treated with EGF, TGF-beta1, or

236 generation of the sub-basal nerve plexus and keratocyte repopulation by 12 months postoperatively.

237 observed at 1 and 3 months, but by 6 months, keratocyte repopulation of the anterior stroma was appar

238 This heterogeneity of keratocyte response may impact wound closure after mecha

239 The RI of quiescent keratocytes (RI = 1.381 +/- 0.004) matched the surroundi

240 Keratocytes sense electric fields and migrate to the cat

241 The keratocytes showed intracytoplasmatic vesicles, whereas

242 Mechanics of motility is simplest in fish keratocytes, so we turned to keratocytes to investigate

243 rum-free media upregulated the expression of keratocyte-specific genes and secreted substantial ECM c

244 rs neurofilament, beta-tubulin III, GFAP; or keratocyte-specific markers keratan sulfate and keratoca

245 pes and movements that are characteristic of keratocytes, suggesting that these mechanisms may serve

246 The very rapid induction of HA expression in keratocytes suggests a functional role of this molecule

247 % of the PMN surface was in contact with the keratocyte surface, and this value decreased to 10% in C

248 hanges in the light-scattering properties of keratocytes that are possibly linked to the abundant exp

249 ssion is a characteristic feature of corneal keratocytes that is lost when cells are phenotypically m

250 In stationary keratocytes, the actin network flow was inwards and radi

251 In nonactivated keratocytes, the expression of alpha3(IV) collagen was d

252 These results show HA secretion by keratocytes to be initiated by a rapid transient increas

253 and interindividually variable distances of keratocytes to Descemet's membrane.

254 In culture, TGFbeta caused cat corneal keratocytes to differentiate into alphaSMA-positive myof

255 F-beta-induced activation of corneal stromal keratocytes to fibroblast- or myofibroblast-phenotype, r

256 s study found HA secretion by primary bovine keratocytes to increase rapidly in response to TGFbeta,

257 implest in fish keratocytes, so we turned to keratocytes to investigate their migration in EFs.

258 aling is an important mechanism for cultured keratocytes to maintain a normal phenotype while continu

259 modified the TGF-beta1-driven transition of keratocytes to myofibroblasts by inhibiting the upregula

260 er, enhancing specific fibrotic responses of keratocytes to TGFbeta.

261 of this molecule in the fibrotic response of keratocytes to wound healing.

262 atively simple cell type-the fish epithelial keratocyte-to define a set of mechanochemical feedback l

263 d to enhanced inflammatory response, stromal keratocyte transactivation, fibrosis, increased p38 mito

264 eal stroma appeared as a region where normal keratocytes transitioned into elongated, hyper-reflectiv

265 ely expressed on cultured rabbit and porcine keratocytes under all conditions examined.

266 Primary human corneal keratocytes under serum-free conditions were used as a m

267 In fish keratocytes undergoing motility, myosin II is concentrat

268 abundant in the corneal basal epithelium and keratocytes until P11.

269 healing corneal wounds, and in vitro corneal keratocytes up-regulate expression of several fibrosis-r

270 tween Descemet's membrane and most posterior keratocytes varied from 2 to 10 and the diameter of coll

271 ation in GCD patient-derived primary corneal keratocytes via homology-directed repair (HDR).

272 thelial cell migration on treated amnion and keratocyte viability after bonding were also measured.

273 RGX did not decrease keratocyte viability.

274 decreased and the number of hyperreflective keratocytes was significantly increased in NK eyes compa

275 ure model of freshly isolated rabbit corneal keratocytes was used.

276 Viable keratocytes were counted on H&E-stained sections 24 hour

277 Primary isolated rabbit corneal keratocytes were cultured in serum-free medium.

278 This degradation was prevented when keratocytes were cultured in the presence of the growth

279 When keratocytes were cultured on AM, the promoter activity o

280 Collagenase-isolated keratocytes were cultured with or without insulin with o

281 ts on the synthesis of collagen and KSPGs by keratocytes were determined.

282 nd ECM production by primary cultured bovine keratocytes were evaluated.

283 Human corneal stromal keratocytes were expanded in transwell culture in the pr

284 When rabbit keratocytes were induced to express the myofibroblast ma

285 Rhesus monkey keratocytes were isolated from central corneal buttons b

286 Stromal keratocytes were observed at variable distances from Des

287 n the presence of the growth factors or when keratocytes were plated at high density.

288 Rabbit corneal keratocytes were plated within standard bovine or rat ta

289 transcription of alpha-SMA mRNA in activated keratocytes were reduced by Rho or ROCK inhibition durin

290 Collagenase-isolated keratocytes were seeded and subcultured on plastic or am

291 Rabbit corneal keratocytes were seeded within collagen matrices that we

292 In another set of experiments, keratocytes were transfected with JNK1/2 Dicer-substrate

293 On the TGF-beta2 background, the keratocytes were treated with IGF-1, and suberoylanilide

294 e independent preparations of cultured human keratocytes were treated with TGF-beta1, to elicit a phe

295 d basal epithelial cells, as well as stromal keratocytes, were assessed by 2 masked observers.

296 We focus on fragments from fish epithelial keratocytes, which are essentially stand-alone motile la

297 pregulated in the absence of beta-catenin in keratocytes, which further triggers ERK1/2 (Mapk3/1) and

298 , in part, by specialized fibroblasts called keratocytes, which reside in the fibrous lamellae of the

299 microscopy showed the presence of activated keratocytes, while the nerve pattern was normal.

300 Similarly, brief treatment of keratocytes with hyaluronidase reduced TGFbeta responses