ライフサイエンスコーパス: kinetoplast

1 volved in replication and segregation of the kinetoplast.

2 tion and segregation of basal bodies and the kinetoplast.

3 ontaining a single enlarged nucleus plus one kinetoplast.

4 selective localization of acriflavin in the kinetoplast.

5 clusively for maintenance of the trypanosome kinetoplast.

6 a decrease in the average amount of DNA per kinetoplast.

7 and localization of the protein only in the kinetoplast.

8 the mitochondrion, with a preference for the kinetoplast.

9 l cycle by inflicting specific damage on the kinetoplast.

10 organized in a complex structure called the kinetoplast.

11 duced population accumulated cells lacking a kinetoplast.

12 tained in a specialized structure termed the kinetoplast.

13 ssion of PNT1 led to cells having mislocated kinetoplasts.

14 the number of nuclei exceeding the number of kinetoplasts.

15 ning aggregated multiple nuclei and multiple kinetoplasts.

16 entification of the polypeptide synthesis in kinetoplasts.

17 e for a functional translation system in the kinetoplasts.

18 ase H activity, that is enriched in isolated kinetoplasts.

19 he typical pattern is two EGJ in G1 phase (1 kinetoplast/1 nucleus, 1K1N) through S-phase (2K1N), dup

20 that had divided their nucleus but not their kinetoplast (2N1K cells).

21 werful trypanocides that act directly on the kinetoplast, a structure unique to the order Kinetoplast

22 croscopy revealed that PNT1 localizes to the kinetoplast, an organelle containing the mitochondrial g

23 ellular level, 23a blocks duplication of the kinetoplast and arrests cytokinesis, making it a new che

24 longer unattached flagellum and repositioned kinetoplast and basal body, reminiscent of epimastigote-

25 of basal body and its associated organelles kinetoplast and Golgi, as well as its role in cytokinesi

26 codes a 70-kDa protein that localizes to the kinetoplast and is essential for both cell growth and ki

27 e phases and rates of morphological changes; kinetoplast and nucleus S phase, division and position,

28 egation of the organelles i.e. basal bodies, kinetoplast and nucleus was disrupted.

29 ervation indicates an uncoupling between the kinetoplast and the nuclear cycle, resulting in cell div

30 s in localization of the protein to both the kinetoplast and the nucleus.

31 yclic-form T. brucei enriched cells with two kinetoplasts and an enlarged nucleus possessing short me

32 interrupted resulting in cells with multiple kinetoplasts and flagella.

33 The kinetoplasts and nuclei were misaligned in the postmitot

34 tream form, however, enriched cells with two kinetoplasts and two nuclei connected through a microtub

35 ment complex linking the basal bodies to the kinetoplast, and a segment of microtubule quartet linkin

36 tion of organelles, including the flagellum, kinetoplast, and nucleus, but were incapable of completi

37 cell showing an extended posterior end, two kinetoplasts, and an enlarged nucleus, apparently the re

38 vents, occurring in the flagellar pocket and kinetoplast, are critical for host-parasite interactions

39 resulting in cells with multiple nuclei and kinetoplasts, as well as multiple detached flagella.

40 To investigate the role of kinetoplast-associated proteins in organizing and conden

41 ructure, we have cloned three genes encoding kinetoplast-associated proteins.

42 uorescence staining showed a single nucleus, kinetoplast, basal body, and flagellum in the anterior p

43 their organelles, such as the mitochondria, kinetoplasts, basal bodies, and flagella, present as sin

44 ures in the trypanosome--the nucleus and the kinetoplast--begin to fluoresce within 1 min of introduc

45 by RNA interference had profound effects on kinetoplast biogenesis in Trypanosoma brucei procyclic c

46 To further explore kinetoplast biology and drug resistance, we screened a g

47 side mammalian cells replicated their single kinetoplast but failed at mitosis.

48 ng anterior flagellum, and either one or two kinetoplasts, but only the anterior kinetoplast was asso

49 ative timing and duration of the nuclear and kinetoplast cell cycle that differs from the current mod

50 m has an increased number of one nucleus-two kinetoplast cells (1N2K) and a small population containi

51 While drug accumulation in the kinetoplast continued after V-ATPase subunit depletion,

52 enes and 3 protein complexes associated with kinetoplast-dependent growth.

53 leate cells (zoids), apparently derived from kinetoplast-dictated cytokinesis and cell division of th

54 ymerase beta at antipodal sites flanking the kinetoplast disc.

55 omplexes positioned on opposite sides of the kinetoplast disc.

56 hows heterogeneous electron densities in the kinetoplast disk.

57 Consistent with kinetoplast dispensability, V-ATPase defective cells wer

58 asizes the role of basal body segregation in kinetoplast division and suggests a possible mechanism f

59 gella in these cells suggested the defect in kinetoplast division arose because of an inhibition of b

60 TbSAS6 at newly forming basal bodies; and 3) kinetoplast division is observed in G2 after completion

61 hesis was relatively unaffected and cell and kinetoplast division proceeded normally for several gene

62 In addition, we identified their role in kinetoplast division which may be also linked to overall

63 NAi in bloodstream trypanosomes also delayed kinetoplast division, and was further observed to inhibi

64 Kinetoplast DNA (kDNA) is a novel form of mitochondrial

65 Kinetoplast DNA (kDNA) is organized into a concatenated

66 ically amplified whole linearized minicircle kinetoplast DNA (kDNA) of the Leishmania subgenus Vianni

67 sitivity of OligoC-TesT with those of nested kinetoplast DNA (kDNA) PCR, nested internal transcribed

68 The multicopy kinetoplast DNA (kDNA) probes were the most sensitive an

69 mitochondrial genome of trypanosomes, termed kinetoplast DNA (kDNA), contains thousands of minicircle

70 Kinetoplast DNA (kDNA), from trypanosomatid mitochondria

71 The trypanosome mitochondrial genome, kinetoplast DNA (kDNA), is a massive network of interloc

72 This genome, known as kinetoplast DNA (kDNA), is organized as a single, massiv

73 escribed a remarkable DNA structure known as kinetoplast DNA (kDNA), isolated from a parasite.

74 otype upon induction of RNAi was the loss of kinetoplast DNA (kDNA), the cell's catenated mitochondri

75 Kinetoplast DNA (kDNA), the form of mitochondrial DNA in

76 Kinetoplast DNA (kDNA), the mitochondrial DNA in kinetop

77 Kinetoplast DNA (kDNA), the mitochondrial DNA of trypano

78 Kinetoplast DNA (kDNA), the trypanosome mitochondrial DN

79 Kinetoplast DNA (kDNA), the trypanosome mitochondrial ge

80 Kinetoplast DNA (kDNA), the unusual mitochondrial DNA of

81 In an RNAi library screen for loss of kinetoplast DNA (kDNA), we identified an uncharacterized

82 usual features, a mitochondrial DNA known as kinetoplast DNA (kDNA).

83 he ability to unknot (decatenate) and cleave kinetoplast DNA (kDNA).

84 he ability to unknot (decatenate) and cleave kinetoplast DNA (kDNA).

85 etwork of minicircles and maxicircles called kinetoplast DNA (kDNA).

86 rlocked circular DNA molecules that form the kinetoplast DNA (kDNA).

87 We evaluated the serum levels of T. cruzi kinetoplast DNA (TckDNA), T. cruzi 18S ribosomal DNA (Tc

88 One striking phenotype was the loss of kinetoplast DNA after interference with expression of a

89 terference (RNAi) of p38 resulted in loss of kinetoplast DNA and accumulation of a novel free minicir

90 than wild type topo II alpha in decatenating kinetoplast DNA and also exhibits a 2-4-fold decrease in

91 approximately equal distribution of parental kinetoplast DNA between daughter kinetoplasts resulted i

92 Nae I-43K decatenated kinetoplast DNA containing nicked circles, implying that

93 y ATP-dependent DNA supercoil relaxation and kinetoplast DNA decatenation assays.

94 ocalized in the antipodal sites flanking the kinetoplast DNA disk, as previously shown in C. fascicul

95 ctivity as judged by an inability to convert kinetoplast DNA from the catenated to the decatenated fo

96 Kinetoplast DNA in African trypanosomes contains a novel

97 The loss of kinetoplast DNA in Leishmania tarentolae, which occurs i

98 kinetoplast to photooxidize selectively the kinetoplast DNA is suggested.

99 fit the working hypothesis that the loss of kinetoplast DNA leads to a respiratory defect which then

100 In one cloned cell line with inducible kinetoplast DNA loss, we found that the RNA interference

101 (RNAi) of TbPIF1 causes a growth defect and kinetoplast DNA loss.

102 l genomic complements from both parents, but kinetoplast DNA maxicircles from one parent.

103 mania parasite contains approximately 10,000 kinetoplast DNA minicircles, which are unequally distrib

104 ing the 75 C-terminal amino acids can rescue kinetoplast DNA missegregation but not the lack of ATOM

105 ultimately led to shrinkage and loss of the kinetoplast DNA network and cessation of growth of the c

106 ity found associated with the mitochondrial, kinetoplast DNA network in trypanosomatid protozoa.

107 ed guide RNAs upon segregation of the single kinetoplast DNA network into daughter cells at cell divi

108 tions with respect to the mitochondrial DNA (kinetoplast DNA network) in this organism are strikingly

109 tenation of plasmid DNA, and decatenation of kinetoplast DNA networks.

110 The final disappearance of the stainable kinetoplast DNA occurred at a cell division in which all

111 ween the complex restriction patterns of the kinetoplast DNA of any of the parasites from Timargara c

112 of these DNAs has not been possible since a kinetoplast DNA primase has not been available.

113 these results suggest a point of control for kinetoplast DNA replication through the regulation of th

114 tor with genes involved in processes such as kinetoplast DNA replication, mitochondrial mRNA synthesi

115 ial protein, which we term p38, functions in kinetoplast DNA replication.

116 ignificant implications for the mechanism of kinetoplast DNA replication.

117 st and is essential for both cell growth and kinetoplast DNA replication.

118 u were tested with a DNA probe directed at a kinetoplast DNA segment of Trypanosoma cruzi.

119 circles to the network and caused a delay in kinetoplast DNA segregation.

120 The kinetoplast DNA sequence of parasitic microorganisms, fo

121 Kinetoplast DNA synthesis involves release of minicircle

122 Kinetoplast DNA synthesis involves release of minicircle

123 The selective inhibition of kinetoplast DNA synthesis was caused by a selective loca

124 During kinetoplast DNA synthesis, minicircles are released from

125 ed by proteolysis of a helicase; the complex kinetoplast DNA system yields a clear view of how mitoch

126 reatly enhanced Topo IIalpha decatenation of kinetoplast DNA to relaxed circular forms.

127 g region of the TOP2 gene, which encodes the kinetoplast DNA topoisomerase, and have carried out dele

128 two kinetoplast ribosomal proteins with the kinetoplast DNA was observed by immunofluorescence, sugg

129 division in which all the remaining visible kinetoplast DNA was retained by one of the daughter cell

130 The mitochondrial DNA (kinetoplast DNA) of the trypanosomatid Crithidia fascicu

131 The mitochondrial DNA (kinetoplast DNA) of the trypanosomatid Crithidia fascicu

132 A time-course of DNA synthesis (nuclear and kinetoplast DNA), duplication of organelles (basal body,

133 ondrial genome of Trypanosoma brucei, called kinetoplast DNA, is a network of topologically interlock

134 have an unusual mitochondrial genome, called kinetoplast DNA, that is a giant network containing thou

135 Kinetoplast DNA, the mitochondrial DNA of Crithidia fasc

136 Kinetoplast DNA, the mitochondrial DNA of trypanosomatid

137 Kinetoplast DNA, the trypanosome mitochondrial genome, i

138 parasitic heterocyclic dications can have on kinetoplast DNA, we have constructed ligation ladders, w

139 ce-based genomic library for cells that lose kinetoplast DNA.

140 necropsy material, using probes specific for kinetoplast DNA.

141 mitochondrion, above and below the condensed kinetoplast DNA.

142 oci, and uniparental retention of maxicircle kinetoplast DNA.

143 c properties that target the minor groove of kinetoplast DNA.

144 t a time corresponding to duplication of the kinetoplast DNA.

145 eplication, we now report that the T. brucei kinetoplast does move relative to the antipodal sites.

146 In this study, we define the complete kinetoplast duplication cycle in T. brucei based on thre

147 axicircle segregation is a late event in the kinetoplast duplication cycle.

148 This new view of the complexities of kinetoplast duplication emphasizes the dependencies betw

149 Kinetoplast duplication was uninterrupted resulting in c

150 ined about the earliest and latest stages of kinetoplast duplication.

151 m for driving the rotational movement of the kinetoplast during minicircle replication.

152 ubunit requirement; this subunit is the only kinetoplast-encoded product ultimately required for viab

153 We identified a kinetoplast-encoded protein, apocytochrome b, whose mRNA

154 With the aim of identification of kinetoplast-encoded proteins we investigated the subunit

155 These results suggest that the kinetoplast enzyme results from processing of the full-l

156 se H activity gel analysis of whole cell and kinetoplast extracts shows that the enzyme is enriched i

157 in and a 45 kDa protein which is enriched in kinetoplast extracts.

158 , growth arrested cells accumulated multiple kinetoplasts, flagella and nucleoli, similar to the effe

159 setse flies and survive without a functional kinetoplast for reasons that have remained unclear.

160 xtracts shows that the enzyme is enriched in kinetoplast fractions.

161 ycle as judged by replication of nuclear and kinetoplast genomes.

162 nied by repositioning of the basal body, the kinetoplast, Golgi, and flagellar pocket, reflecting an

163 cient cells contained multiple basal bodies, kinetoplasts, Golgi, and nuclei.

164 idence for translation of these mRNAs in the kinetoplast has been missing for decades.

165 key veterinary drug that accumulates in the kinetoplast in African trypanosomes.

166 an aggregate of multiple nuclei and multiple kinetoplasts in an undivided cell.

167 Kinetoplast independence and isometamidium resistance ar

168 ccumulation of cells with two nuclei and two kinetoplasts, indicating that cytokinesis was specifical

169 Segregation of the kinetoplast is hampered in trypanosomes treated with the

170 The kinetoplast itself contains the mitochondrial genome, co

171 depends on maintenance and expression of its kinetoplast (kDNA), the mitochondrial genome of this par

172 ted daughter cells, each containing a single kinetoplast known as a zoid.

173 f anucleated cells, each containing a single kinetoplast known as the zoids (0N1K).

174 re-positioned to a location posterior to the kinetoplast, leading to defects in cytokinesis and the g

175 in a kDNA independent cell line resulted in kinetoplast loss but was viable, indicating that PNT1 is

176 TPase subunit depletion, acriflavine-induced kinetoplast loss was specifically tolerated in these cel

177 ll division, including mispositioning of the kinetoplast, loss of flagellar connection, and preventio

178 cating that PNT1 is required exclusively for kinetoplast maintenance.

179 ecombination, and uniparental inheritance of kinetoplast maxicircle DNA.

180 ides were identified by fractionation of the kinetoplast membranes, labeled with [(35)S]methionine an

181 le for PLK kinase activity in basal body and kinetoplast migration.

182 The screening primers recognize kinetoplast minicircle DNA of all Leishmania species.

183 eloped to amplify the variable region of the kinetoplast minicircles of all Leishmania species which

184 rome c oxidase (respiratory complex IV) from kinetoplast mitochondria of the trypanosomatid protozoan

185 ts a unified model for RNA processing in the kinetoplast mitochondria.

186 nd apocytochrome b (Cyb) was investigated in kinetoplast-mitochondria of Leishmania.

187 The type of RNA editing found in the kinetoplast-mitochondria of trypanosomes and related pro

188 i, the first trans-acting factor involved in kinetoplast mitochondrial transcription to be identified

189 88 treatment inhibits DNA replication in the kinetoplast (mitochondrial nucleoid) and nucleus.

190 A editing systems have been described in the kinetoplast-mitochondrion of trypanosomatid protists.

191 Kinetoplast motion of either type must facilitate orderl

192 he kDNA disk, a pattern that did not suggest kinetoplast motion.

193 The two kinetoplasts moved apart from each other but stopped jus

194 cleus, apparently the result of an inhibited kinetoplast multiplication and a failed mitosis.

195 plication of organelles (basal body, bilobe, kinetoplast, nucleus), and cytokinesis was obtained.

196 ells with abnormal complements of nuclei and kinetoplasts, often with the number of nuclei exceeding

197 a topologically intricate mitochondrial DNA (kinetoplast or kDNA) in the form of a network of thousan

198 e topologically intricate mitochondrial DNA (kinetoplast or kDNA) of Trypanosoma brucei brucei and re

199 cked at the local level, even as the overall kinetoplast organization is dramatically altered.

200 muM), inducing a typical multiple nuclei and kinetoplast phenotype without being generally cytotoxic.

201 ptide repeat-containing RNA binding protein, kinetoplast polyadenylation factor 3 (KPAF3), and demons

202 ide repeat-containing (PPR) proteins, termed kinetoplast polyadenylation/uridylation factors (KPAFs)

203 han 30 years, and although a large number of kinetoplast replication genes and proteins have been ide

204 rent triple function in coupling mitosis and kinetoplast replication with cytokinesis in T. brucei.

205 tified protein that couples both mitosis and kinetoplast replication with cytokinesis in the trypanos

206 le, possibly through an inhibitory effect on kinetoplast replication.

207 the antipodal sites is only observed during kinetoplast replication.

208 of parental kinetoplast DNA between daughter kinetoplasts resulted in a decrease in the average amoun

209 and a colocalization of REH1, REL1, and two kinetoplast ribosomal proteins with the kinetoplast DNA

210 Whereas the C. fasciculata kinetoplast rotates, that from T. brucei oscillates.

211 We show that kinetoplast S phase occurs concurrently with the reposit

212 including a cell-cycle arrest due in part to kinetoplast segregation defects.

213 cycle, resulting in cell division driven by kinetoplast segregation with neither a priori S phase no

214 driven into cytokinesis and cell division by kinetoplast segregation without a completed mitosis, the

215 unosorbent assay [ELISA], Crithidia luciliae kinetoplast staining).

216 +/+, but not -/-, mice by Crithidia luciliae kinetoplast staining.

217 s encoded by both the nuclear genome and the kinetoplast, the unusual mitochondrial genome of these p

218 ponsible for positioning and segregating the kinetoplast--the mitochondrial genome.

219 selective localization of acriflavin in the kinetoplast to photooxidize selectively the kinetoplast

220 nit of nuclear replication protein A and the kinetoplast topoisomerase II, accumulate periodically du

221 ence, thus indicating that the corresponding kinetoplast unedited mRNA is translated into a functiona

222 e or two kinetoplasts, but only the anterior kinetoplast was associated with the flagellum.

223 ei, while in cells overexpressing PLKty, the kinetoplast was mainly found at the posterior end of the

224 Following PLK depletion, the single kinetoplast was predominantly located between the two di

225 early half of the TbCen3 depleted cells, the kinetoplasts were enlarged and undivided.

226 ivalent of DNA per nucleus and three or four kinetoplasts were produced.

227 an African Trypanosomiasis, HAT), contains a kinetoplast with the mitochondrial DNA (kDNA), comprisin