ライフサイエンスコーパス: kiss-and-run

1 cargo discharge and reducing pore closure ('kiss-and-run').

2 connection between the vesicle and surface ('kiss-and-run').

3 sis was shifted from full-collapse fusion to kiss-and-run.

4 n pathway via alternative mechanisms such as kiss-and-run.

5 via two distinct mechanisms: full-fusion and kiss-and-run.

6 s to indicate that synaptic vesicles undergo kiss-and-run.

7 Kiss-and-run and reuse could enable hippocampal nerve te

8 k single vesicles through multiple rounds of kiss-and-run and reuse, without perturbing vesicle cycli

9 vents associated with "full fusion" events, "kiss-and-run" and "kiss-and-stay" exocytosis, confirming

10 ivities, their preference for full fusion or kiss-and-run, and their sensitivity to inhibition by syn

11 action of fusion events has been shown to be kiss-and-run, as determined using cell-attached capacita

12 The increased incidence of kiss-and-run at lower frequencies may ensure that vesicl

13 The only genetic evidence for kiss-and-run at the synapse comes from mutations in the

14 meter, Qdots will not escape vesicles during kiss-and-run but only with full collapse fusion.

15 ible fusion process commonly referred to as 'kiss-and-run', but only rarely.

16 Alternatively, the pore may close (kiss-and-run), but the triggering mechanisms and its end

17 pocampal synapses and that the prevalence of kiss-and-run can be modulated by stimulus frequency.

18 Kiss-and-run dominated at the beginning of stimulus trai

19 The importance of kiss-and-run during efficient neurotransmission has rema

20 cape of FM 4-64, indicating that it is a non-kiss-and-run endocytic event.

21 forms of compensatory endocytosis, including kiss-and-run endocytosis and a mechanism for efficient r

22 Mechanisms, including rapid kiss-and-run endocytosis as well as local, preferential

23 tion of endophilin mutants demonstrates that kiss-and-run endocytosis is a major component of synapti

24 sed including clathrin-mediated endocytosis, kiss-and-run endocytosis, cavicapture, and bulk endocyto

25 his form of recycling is not compatible with kiss-and-run endocytosis; moreover, it is 200-fold faste

26 By extension, clathrin-independent "kiss-and-run" endocytosis does not sustain synaptic tran

27 ely 1 s by the reversal of fusion pores via 'kiss-and-run' endocytosis.

28 ion of openings that close without dilating (kiss-and-run events) enabled us to resolve exocytosis in

29 ) IV increased the frequency and duration of kiss-and-run events, but left their amplitude unchanged.

30 y synaptic vesicle endocytosis including any kiss-and-run events.

31 and run" pathway, and that the fraction of "kiss and run" events can be increased to over 80% by sup

32 "Kiss-and-run" events and tubule connections mediate tran

33 me and revealed to precisely mark organelle "kiss-and-run" events.

34 radox is explained by a fourfold increase in kiss-and-run exocytosis (as determined by single-granule

35 he upregulation of STXBP6 and an increase in kiss-and-run exocytosis at the expense of full fusion.

36 synaptotagmin isoform activated, and because kiss-and-run exocytosis can filter small molecules throu

37 ely slow rate of release of glutamate during kiss-and-run exocytosis shifts the population of AMPA re

38 ion pores and survive intact for future use (kiss-and-run exocytosis).

39 f a fusing vesicle are fates associated with kiss-and-run exocytosis, and we find that these are the

40 An intact cortex favors kiss-and-run exocytosis, whereas disrupting the cortex f

41 dent partial emptying of DCVs, suggestive of kiss-and-run exocytosis.

42 incomplete exocytosis, often referred to as 'kiss and run' exocytosis.

43 ore fleeting mode of vesicle fusion, termed 'kiss-and-run' exocytosis or 'flicker-fusion', indicates

44 tsynaptic consequences, such that so-called 'kiss-and-run' exocytosis results in negligible activatio

45 In contrast, a nonclassical mode known as "kiss-and-run" features fusion by a transient fusion pore

46 ) in Dictyostelium is carried out by a giant kiss-and-run focal exocytic event during which the two m

47 uminescence change allowed us to distinguish kiss-and-run from full-collapse fusion and to track sing

48 selectively release catecholamines through a kiss-and-run fusion event.

49 l cortex plays a key role in stabilizing the kiss-and-run fusion event.

50 rimental evidence supports a predominance of kiss-and-run fusion events and rapid vesicular re-use.

51 Kiss-and-run fusion events were concentrated near the ce

52 cle fusion, we found both full collapse and 'kiss-and-run' fusion at calyx-type synapses.

53 These results show that 'kiss-and-run' fusion occurs at synapses and that it can

54 ' remains controversial, and the ability of 'kiss-and-run' fusion to generate rapid synaptic currents

55 'Kiss-and-run' fusion was seen as a brief capacitance fli

56 es completely, or close rapidly to generate 'kiss-and-run' fusion.

57 Fusion pores seen during microvesicle kiss-and-run have a conductance of 19 pS, 11 times small

58 t evidence argues against the occurrence of 'kiss-and-run' in hippocampal synapses.

59 ne invagination and vesicle reformation; (b) kiss-and-run, in which the fusion pore opens and closes;

60 Transient "kiss and run" interactions between endosomes containing

61 Further work is needed to determine whether kiss-and-run is a major mode of fusion and has a major r

62 Kiss-and-run is a mode of membrane fusion and retrieval

63 Our data also provide further evidence that kiss-and-run is able to maintain neurotransmitter releas

64 echnique to provide compelling evidence that kiss-and-run is the dominant mode of vesicle fusion at h

65 onclusions dispute previous assertions that "kiss-and-run" is a major mechanism of vesicle recycling

66 Transient fusion ("kiss-and-run") is accepted as a mode of transmitter rele

67 tion of flickering and closing fusion pores (kiss-and-run) is very well explained by the observed beh

68 as that proposed to support the presence of kiss-and-run, is likely explained by the stochastic natu

69 Kiss-and-run (KR) is an unconventional fusion between se

70 egulation between full fusion and reversible kiss-and-run-like events.

71 We propose that kiss-and-run maintains neurotransmission at active zones

72 ese data suggest the presence of a selective kiss and run mechanism of insulin release.

73 thin the readily releasable pool (RRP) via a kiss-and-run mechanism that involves rapid opening and c

74 RE is associated with the transient fusion ("kiss-and-run") mechanism of transmitter release and is t

75 ypothesis that vesicle secretion involves a 'kiss-and-run' mechanism.

76 Basal sympathetic firing elicits a transient kiss-and-run mode of exocytosis and modest catecholamine

77 pore dilation and maintains the granule in a kiss-and-run mode of exocytosis.

78 th low release probability primarily use the kiss-and-run mode, whereas high release probability term

79 ween exocytosis and endocytosis, inducing a "kiss and run" mode of exocytosis and endocytosis.

80 These experiments have revealed a "kiss and run" mode of exocytosis in which synaptic vesic

81 The extent to which a "kiss-and-run" mode of endocytosis contributes to synapti

82 tch from a full fusion mode of release to a "kiss-and-run" mode of release through the transient open

83 s of vesicle retrieval: a fast (400-860 ms) 'kiss-and-run' mode that has a selective fusion pore; a s

84 Work supporting the kiss-and-run model of transient exocytosis implies that

85 Consistent with a kiss-and-run or cavicapture mode of secretion, biotinyla

86 hormones are released through a transient ('kiss and run') or an irreversibly dilating pore (full fu

87 ucture (Omega-profile), followed by closure (kiss-and-run) or merging of the Omega-profile into the p

88 about 20% of the vesicles normally use this "kiss and run" pathway, and that the fraction of "kiss an

89 they released their contents, indicating a "kiss-and-run" pathway.

90 frequent cycles of fusion and fission in a 'kiss and run' pattern.

91 cles, with long-dwelling vesicles preferring kiss-and-run rather than full-collapse fusion.

92 , including bulk retrieval and the so-called kiss-and-run recycling.

93 rom the reserve pool, placing constraints on kiss-and-run recycling.

94 ve terminals but do not preclude concurrent "kiss-and-run" recycling.

95 However, the existence of kiss-and-run remains highly controversial, as revealed b

96 es, the size of the fusion pore is unclear, 'kiss-and-run' remains controversial, and the ability of

97 parison with FM dye destaining revealed that kiss-and-run strongly prevailed over full-collapse fusio

98 During kiss-and-run, syt IV increased the conductance and durat

99 -fold higher with Syt I than Syt IV, but for kiss-and-run the Ca2+ sensitivities differed by a factor

100 During "kiss and run," the vesicle interior may be exposed very

101 st 'local cycling' near release sites (e.g. 'kiss and run' transmitter release) at low stimulus frequ

102 otein receptor (SNARE) complex that promotes kiss-and-run vesicle fusion.

103 Quickening of kiss-and-run vesicle reuse was also observed at higher f

104 tention of membrane marker, consistent with 'kiss-and-run' vesicle cycling.

105 Such 'kiss-and-run' vesicle fusion can in principle result in

106 Alternatively, in what is termed kiss-and-run, vesicles can release transmitter during tr

107 of a Ca2+ ligand in the C2A domain of Syt I; kiss-and-run was inhibited by mutation of a homologous C

108 retory vesicle collapses into the PM; or by "kiss-and-run," where the fusion pore does not dilate and

109 cycled by a second, faster mechanism called 'kiss-and-run', which operates in 1 s or less to retrieve

110 pore, the activation of isoforms that favor kiss-and-run will select smaller molecules over larger m

111 id opening and closing of a fusion pore (or "kiss-and-run") with a median opening time of 2.6 s, whic

112 regulates the choice between full fusion and kiss-and-run, with Ca2+ binding to the C2A and C2B domai

113 Nonclassical fusion retrieval by kiss-and-run would be kinetically advantageous but remai