ライフサイエンスコーパス: knock-in

1 nized mouse model (hCD1d-KI) with human CD1d knocked in.

2 NKX2-5 homeodomain was replicated in mice by knocking in a comparable missense mutation.

3 3ER(TAM) fusion protein was expressed from a knock-in allele (Th-MYCN/Trp53(KI)).

4 Pre-leukemic mice with the Flt3(ITD) knock-in allele manifested an expansion of classical DCs

5 eted NRP1 ablation in RGCs with a Brn3b(Cre) knock-in allele reduced axonal midline crossing at the o

6 thogenesis, we generated a conditional Tor1a knock-in allele that is converted from wild-type to DYT1

7 apply these methods to the generation of GFP knock-in alleles and gene replacements without co-integr

8 alyzed islet function in mice homozygous for knock-in alleles encoding deacetylated FoxO1 (6KR).

9 of Drosophila E-Cadherin (DE-Cad) endogenous knock-in alleles that carry mutations targeting this hig

10 les via nonhomologous end joining (NHEJ) and knock-in alleles via homology-directed repair (HDR).

11 Using newly generated conditional knock-in and knockout mice, we show in this study that l

12 hine analgesia was equivalent when tested in knock-in and wild-type mice.

13 ng two new mouse models: a conditional E401K knock-in, and a conditional knockout animal.

14 Our work presents tools to nuclease-mediated knock-in animal production, and sheds light on improving

15 The SPAK CCT domain defective knock-in animals are viable, and the Leu502Ala mutation

16 Using a knock-in approach, we show that all four genes are expre

17 Furthermore, targeted mutational knock-in at single alleles to model diseases caused by h

18 ved voltage indicator, QuasAr2, via targeted knock-in at the rosa26 locus.

19 Using a mouse model whose knock-in BCR does not functionally engage with immunizin

20 asticity in the CEm neurons of BDNF Val66Met knock-in (BDNF(Met/Met)) mice.

21 We present a method called knock-in blunt ligation for exploiting these breaks to i

22 Mice with a knock-in CaMKIIalpha-E183V mutation have lower total for

23 Foxp3-GFP knock-in Cd39(+/+) and Cd39(-/-) mice were used to sort

24 nerality of this proposal by using a genetic knock-in cell fate mapping strategy in different murine

25 We show here that for our GFP-p65 knock-in cells NF-kappaB behaves as a damped oscillator

26 own cells and FBXL2-insensitive IP3R3 mutant knock-in clones display increased cytosolic Ca(2+) relea

27 We also efficiently obtained biallelic knock-in clones, using single-stranded oligodeoxynucleot

28 muscle, homozygous A302 mice (A/A) and their knock-in controls (S/S) exhibited similar glucose homeos

29 However, transgenic and knock-in Cre drivers used to perform lineage tracing exp

30 We show that the 'knock-in' Crouzon mouse model Fgfr2c(C342Y/C342Y) carryi

31 proteins can be used to maximize knockout or knock-in editing efficiencies or to balance editing effi

32 tion of an HDR enhancer, RS-1, increases the knock-in efficiency by two- to five-fold at different lo

33 air (HDR) will improve the nuclease-mediated knock-in efficiency.

34 effects of Cas9 and increased point mutation knock-in efficiency.

35 Mice with a knock-in F77I mutation in the GABAA receptor gamma2 subu

36 cells at the single-cell level using Bcl11b knock-in fluorescent reporter mice.

37 rget site confirmed that Cas9 RNPs generated knock-in genome modifications with up to approximately 2

38 al abnormalities occur in aged G2019S mutant knock-in (GKI) mice.

39 kb of cis-regulatory genomic space and uses knock-in green fluorescent protein (GFP) reporters to re

40 ropose that further analyses in the paternal knock-in H19(+/hIC1) mice will elucidate the molecular m

41 ssion of marker genes by BAC transgenesis or knock-in has generated useful transgenic mouse lines.

42 ccompanies motivational deficits in the Q175 knock-in HD mouse model.

43 nalysis of the endogenous gamma1 locus and a knock-in heterologous promoter in mice identified the pr

44 ociated virus (AAV)-mediated gene editing to knock in HLA-E genes at the B2M locus in human PSCs in a

45 ted and phenotypically characterized a novel knock-in humanized mouse model carrying the severe, MECD

46 (HITI) strategy, which allows for robust DNA knock-in in both dividing and non-dividing cells in vitr

47 Importantly, knock-in in several non-rodent species has been finally

48 mug/mL in MBL2(+/+)Mbl1(-/-)Mbl2(-/-) [MBL2 knock in (KI)] mice.

49 e models of MMAuria using a constitutive Mut knock-in (KI) allele based on the p.Met700Lys patient mu

50 umulation was significantly reduced in APOE2 knock-in (KI) animals and was significantly increased in

51 is issue, we have generated MLL4 enzyme-dead knock-in (KI) embryonic stem (ES) cells and mice, which

52 In this study, we used knock-in (KI) mice (Pnpla3(148M/M) ) to examine the mech

53 n this study, we used TTT/AAA beta2-integrin knock-in (KI) mice and TCR-transgenic (OT-II) KI mice, i

54 Genetically engineered knock-in (KI) mice containing the A8V mutation (heterozy

55 Here, we used knock-in (KI) mice expressing an Itgb3 variant that phen

56 We previously reported that female apoE4 knock-in (KI) mice had an age-dependent decline in hilar

57 We find that hearts of Cys42Ser PKGIalpha knock-in (KI) mice, which are resistant to PKGIalpha oxi

58 (WT) or disulfide-resistant C42S PKG Ialpha knock-in (KI) mice.

59 exosome secretion from astrocytes in HD140Q knock-in (KI) mice.

60 We generated a D477G knock-in (KI) mouse and characterized its phenotypes.

61 we administered low-dose oral rapamycin to a knock-in (KI) mouse model of authentic mtDNA disease, sp

62 yzed the T-cell compartment in a conditional knock-in (KI) mouse model of mutant Idh1.

63 rated a colon-specific inducible Car1(CreER) knock-in (KI) mouse with broad Cre activity in epithelia

64 hanges after TBI in an AD model, the APP/PS1 knock-in (KI) mouse.

65 S tau transgenic mice on either a human ApoE knock-in (KI) or ApoE knockout (KO) background, here we

66 hTau knock-in (KI) proteins were expressed at normal, endogen

67 kLANA knock-in (KLKI) MHV68 was replication competent in vitro

68 erred mechanism for common applications such knock-in, knock-out or precise mutagenesis, but remains

69 t a novel optogenetic approach using a mouse knock-in line with conditional expression of channelrhod

70 fects modulated by exercise in BDNF Val66Met knock-in male mice.

71 P1 knock-in megakaryocyte progenitors have reduced prolifer

72 Disruption of Sall1-NuRD in vivo in knock-in mice (DeltaSRM) resulted in accelerated differe

73 n in the synaptic vesicle cycle, we produced knock-in mice (Otof(Ala515,Ala517/Ala515,Ala517)) with l

74 Because homozygous Gpx4 knock-in mice (Sec46Ala-Gpx4(+/+)) are not viable we cre

75 RASSF1A levels were reduced in PML/RARalpha knock-in mice and APL patient samples.

76 We generated CUGBP1-S302A knock-in mice and found that the reduction of translatio

77 -liposome vaccine in 2F5 bnAb VHDJH and VLJL knock-in mice and rhesus macaques modified KYNU activity

78 Here we investigated astrocytes from HD140Q knock-in mice and uncovered evidence that mHtt decreases

79 H activity in striatal cells derived from HD knock-in mice and YAC128 mice.

80 We generated heterozygous STING N153S knock-in mice as a model of SAVI.

81 Here, we establishRnaseh2b(A174T/A174T)knock-in mice as a subclinical model of disease, identif

82 ioblastoma multiforme (GBM)-prone H-Ras(12V) knock-in mice as well as in glioma cell lines and patien

83 n of EPRS phosphorylation, we generated Eprs knock-in mice bearing phospho-deficient Ser999-to-Ala (S

84 ed phospholamban (PLN)-deficient S2814D(+/+) knock-in mice by crossing two colonies, S2814D(+/+) and

85 ed phospholamban (PLN)-deficient/S2814D(+/+) knock-in mice by crossing two colonies, S2814D(+/+) and

86 ning this approach with an allelic series of knock-in mice carrying frequent RTT-associated mutations

87 The P56S Vapb knock-in mice could be a valuable tool to gain a better

88 Significantly, the knock-in mice demonstrate accumulation of P56S VAPB prot

89 Here, we show that Rag2(R229Q) knock-in mice developed an inflammatory bowel disease af

90 In naive CD4bs, unmutated common ancestor knock-in mice Env(+)B cell clones develop anergy and par

91 Livers of Nlrp3(A350V) knock-in mice exhibited severe liver inflammatory change

92 We generated Foxn1 knock-in mice expressing a C-terminal hemagglutinin-tagg

93 receptor and Galphaq KO mice, as well as in knock-in mice expressing a mutant Ala(286)-CaMKIIalpha t

94 We found previously that knock-in mice expressing a ubiquitin-binding-defective m

95 ispensable for dystroglycan function because knock-in mice expressing binding-deficient T190M dystrog

96 In contrast, crossing ABIN1[D485N] mice to knock-in mice expressing catalytically inactive mutants

97 Here we used knock-in mice expressing catalytically inactive prostasi

98 and hippocampal axonal sprouting observed in knock-in mice expressing FAD-linked PS1 mutation.

99 to VRC01-class bnAb precursors and immunized knock-in mice expressing germline-reverted VRC01 heavy c

100 Immunization experiments in knock-in mice expressing gl-VRC01 or gl-PGT121 show that

101 To address this question we employed Gpx4 knock-in mice expressing the Sec46Ala-Gpx4 mutant, in wh

102 r drugs) technology by creating ROSA26-based knock-in mice for the conditional expression of a Gs-cou

103 Here we generated and analyzed Pten knock-in mice harboring a C2 domain missense mutation at

104 Furthermore, in knock-in mice harboring constitutively active eNOS, elev

105 nfection with M. smegmatis, macrophages from knock-in mice harboring R753Q TLR2 expressed lower level

106 These knock-in mice have NOP receptors that function both in v

107 We generated Abca4(PV/PV) knock-in mice homozygous for the complex PV allele to in

108 ient for sterol-accelerated degradation, and knock-in mice in which endogenous HMGCR harbors mutation

109 l importance of the CCT domain, we generated knock-in mice in which the critical CCT domain Leu502 re

110 To address these issues, we have created knock-in mice in which the pH-sensitive green fluorescen

111 forts to refine these models, we constructed knock-in mice in which the second extracellular loops of

112 a majority of transcript level changes in HD knock-in mice involve alteration of the rate of mRNA pro

113 "beta3DeltaRGT" knock-in mice lack the 3 C-terminal beta3 tail residues,

114 In anti-thymocyte/Thy-1 autoreactive BCR knock-in mice lacking self-Thy-1 ligand, immunoglobulin

115 ce, double-mutant Kit(V558Delta;Y567F/Y567F) knock-in mice lacking the SRC family kinase-binding site

116 The homozygous knock-in mice mimic the clinical phenotypes of RP, inclu

117 hosphorylated in Atoh1's bHLH domain in vivo Knock-in mice of both sexes bearing a GFP-tagged phospho

118 appropriate tissues, we have generated Vapb knock-in mice replacing wild-type Vapb gene with P56S mu

119 Sustained GSK3 activity in GSK3(S/A) knock-in mice reportedly accelerates peripheral nerve re

120 We conclude that human IL-6 knock-in mice represent a novel and improved model for h

121 photobleaching in slices from VGLUT1(Venus) knock-in mice reveal 75% of VGLUT1-containing vesicles h

122 Heterozygous green fluorescent protein (GFP)-knock-in mice revealed rapid induction of gene expressio

123 ry.In vitroandin vivoanalysis of GSK3 single knock-in mice revealed the unexpected contribution of GS

124 The SPAK CCT domain knock-in mice showed typical features of Gitelman Syndro

125 HCV entry factor knock-in mice take up HCV with an efficiency similar to

126 igible expression of truncated Pol iota, and knock-in mice that express full-length Pol iota that is

127 ells or lungs from Prdx6-null or Prdx6-D140A-knock-in mice that lack the phospholipase A2 activity (P

128 is hypothesis, gain-of-function Nlrp3(A350V) knock-in mice were bred onto il17a and Tnf knockout back

129 racterize receptor location and trafficking, knock-in mice were created by inserting the gene encodin

130 Knock-in mice were susceptible to passive and active ana

131 n of receptor localization, we have produced knock-in mice with a fluorescent-tagged NOP receptor in

132 Treatment of SBMA knock-in mice with clenbuterol, which was started at dis

133 CE1 S-palmitoylation through the analysis of knock-in mice with cysteine-to-alanine substitution at t

134 e (HD), we generated an allelic series of HD knock-in mice with graded levels of phenotypic severity

135 brain and peripheral tissue samples from HD knock-in mice with increasing CAG repeat lengths.

136 Adult transgenic GSK3alpha(S/A)/beta(S/A) knock-in mice with sustained GSK3 activity show markedly

137 iated gene editing was used to produce Reep6 knock-in mice with the p.Leu135Pro RP-associated variant

138 We have now generated knock-in mice with two different point mutations in Nefl

139 In Foxp3DTR knock-in mice, about 99% Treg depletion was achieved, re

140 ML/RARalpha oncogene in cells and transgenic knock-in mice, an observation confirmed and extended by

141 Here, we constructed Pol beta R137Q knock-in mice, and found that homozygous knock-in mouse

142 he myeloproliferative phenotypes in FLT3-ITD knock-in mice, and significantly prolonged the survival

143 rst reports of Mecp2[R133C] and Mecp2[T158M] knock-in mice, in addition to Mecp2[R306C] mutant mice.

144 of scopolamine were assessed in BDNF Val/Met knock-in mice, in which BDNF processing and release are

145 yocytic maturation is not affected in the P1 knock-in mice, suggesting that RUNX1B can regulate endom

146 Using our newly generated CD11a-mYFP knock-in mice, we discovered that naive CD8(+) T cells r

147 hose isolated from genome edited mTOR S2159A knock-in mice, we show that mTOR S2159 phosphorylation p

148 in the peripheral lymph nodes of transgenic knock-in mice, where the IL-15Ralpha intracellular signa

149 om young hetero- and homozygous R349P desmin knock-in mice, which carry the orthologue of the most fr

150 AOC3-knock-in mice, which express a catalytically inactive fo

151 uced OVA-specific B cells only in human IL-6 knock-in mice.

152 ation was significantly compromised in these knock-in mice.

153 eactive astrocytes in the striatum of HD140Q knock-in mice.

154 t increased the platelet counts in Jak2V617F knock-in mice.

155 mislocalization and cone degeneration in the knock-in mice.

156 tion with CX3CR1(GFP/WT);CCR2(RFP/WT) double knock-in mice.

157 ls, and gene-targeting for the generation of knock-in mice.

158 This effect is lost in S1928A knock-in mice.

159 cal neurons in wild-type and parvalbumin-Cre knock-in mice.

160 d ALDH2-deficient, ALDH2*1/*2, heterozygotic knock-in mice.

161 oblasts derived from pathogenic LRRK2-R1441G knock-in mice.

162 (LVH), which is reproduced in Raf1(L613V/+) knock-in mice.

163 cells derived from mice and patients, and in knock-in mice.

164 tions on hematopoiesis and leukemogenesis in knock-in mice.

165 ured in B cells from heterozygous TACI A144E knock-in mice.

166 A mouse knock-in model expressing the patient mutation N1768D re

167 mine 101-positive astrocytes of Q175 mice, a knock-in model of Huntington's disease (HD).

168 In this study, using a murine knock-in model, we investigated the mechanisms underlyin

169 In this study, we employed two knock-in models to directly compare the mutability of co

170 Using reconstitution and knock-in models, we report that mutation of key amino ac

171 raction, we generated an EPCR point mutation knock-in mouse (EPCR(R84A/R84A)) which lacks the ability

172 BDNF release and early neuropathology in HD knock-in mouse brain.

173 ted the repeat expansion in the variant SCA3 knock-in mouse by cell-type specific Cre/LoxP recombinat

174 thways downstream of NOD2, we created a Nod2 knock-in mouse carrying the most common mutation seen in

175 A "redox-dead" knock-in mouse containing a C43S mutation exhibits pheno

176 37Q knock-in mice, and found that homozygous knock-in mouse embryos were typically small in size and

177 In R137Q knock-in mouse embryos, the BER efficiency was severely

178 after antigenic stimulation, we generated a knock-in mouse expressing a modified form of the Cameleo

179 We subsequently generated an HAQ knock-in mouse expressing a mouse equivalent of the HAQ

180 ossing Pten conditional knockout mice with a knock-in mouse expressing the Cre recombinase in the CD4

181 y macrophages from a Zfp36-V5 epitope tagged knock-in mouse generated by CRISPR/Cas9-mediated genome

182 We generated the first patient-mimicking knock-in mouse harbouring the most common disease-causin

183 We generated a knock-in mouse in which the endogenous H19/Igf2 ICR (mIC

184 Using a PTEN knock-in mouse lacking the PDZ motif, and a cell-permeab

185 or Exclusively Activated by a Designer Drug) knock-in mouse line to manipulate intracellular Ca(2+) l

186 Here we characterized a Sox2-CreER knock-in mouse line with two independent reporter mouse

187 rs and defects in spindle orientation in our knock-in mouse line, which are absent in knockout animal

188 ic changes in other organs, we generated two knock-in mouse lines on the C57BL/6N background harborin

189 Furthermore, we created a FIP200-4A mutant knock-in mouse model and found that specifically blockin

190 A knock-in mouse model carrying the homologous p.Tyr97Cys

191 A knock-in mouse model carrying the patient mutation p.Asn

192 p53 was further validated in vivo by using a knock-in mouse model expressing an acetylation-mimicking

193 We used a knock-in mouse model expressing the ACTA1 His40Tyr actin

194 We generated a knock-in mouse model harboring the c.365 G>C Fhl1 mutati

195 Here we demonstrate, using a knock-in mouse model in which GFRalpha1 is no longer loc

196 To address this, we generated a Brca2 knock-in mouse model lacking exons 4-7 and demonstrated

197 ies CAG length-dependent phenotypes in a Htt-knock-in mouse model of HD.

198 these limitations, we generated an inducible knock-in mouse model of iRFP713.

199 ter understand these defects, we generated a knock-in mouse model of l-DOPA-responsive dystonia (DRD)

200 and early cone degeneration in the mutation knock-in mouse model of LCA.

201 We have developed a knock-in mouse model of OPMD (Pabpn1+/A17) that contains

202 ore, EndMT is an early event in a JAK2-V617F knock-in mouse model of primary myelofibrosis.

203 Using a knock-in mouse model of the FAAH polymorphism that contr

204 Here, we characterized a novel knock-in mouse model that expresses mouse HD gene homolo

205 n this study, we used the gp130(F/F) (Il6st) knock-in mouse model to examine the pathogenic contribut

206 ze et al use a tetracycline-inducible Dnmt3b knock-in mouse model to investigate how DNMT3B-mediated

207 A LGMD2I knock-in mouse model was generated to express the most f

208 independent effects of SSRIs, we developed a knock-in mouse model whereby high-affinity interactions

209 We created a knock-in mouse model with an RNase H2 AGS mutation in a

210 role in disease pathogenesis, we generated a knock-in mouse model with NB disruption mediated by 2 po

211 We generated a novel Shank3 conditional knock-in mouse model, and show that re-expression of the

212 sorders and profound deafness, and studied a knock-in mouse model, Ush1c c.216G>A, for Usher syndrome

213 thylation levels in a tetracycline-inducible knock-in mouse model.

214 vector to express IVS-AAA in the brain of a knock-in mouse model.

215 al neurogenesis in Huntington's disease (HD) knock-in mouse models and associated embryonic stem cell

216 Here we show, using homozygous knock-in mouse models in which we replace murine Mc3r wi

217 e STN was studied in BAC transgenic and Q175 knock-in mouse models of HD.

218 l to CAG repeat length and is present in all knock-in mouse models of Huntington's disease (HD) with

219 r phenotypes in the striatum of six distinct knock-in mouse models of the HD mutation.

220 ion sites in IDH1 and IDH2 using conditional knock-in mouse models.

221 We include two SCA3 knock-in mouse models: our previously published model th

222 By using primary CTLs derived from a knock-in mouse of the CG membrane protein Synaptobrevin2

223 uman signal regulatory protein alpha (SIRPA) knock-in mouse on a Rag2(-/-) Il2rg(-/-) background (SRG

224 We believe that the G37S knock-in mouse provides an excellent animal model for st

225 ion-dependent proteasomal degradation in the knock-in mouse RPE due to misfolding.

226 We recently described a knock-in mouse strain in which the substitution of 2 ami

227 oped a Flag- and hemagglutinin-tagged Fancd2 knock-in mouse strain that allowed a high throughput mas

228 A FMF-knock-in mouse strain that expresses chimeric pyrin prot

229 For example, recent knock-in mouse studies have shown that MDM2 heterooligom

230 We developed a knock-in mouse that biologically recapitulates a common

231 nerated a floxed tandem dye (td)Tomato-C5aR2 knock-in mouse that we used to track C5aR2 expression in

232 A knock-in mouse was produced that replaced GFRalpha1 with

233 The C5aR2 knock-in mouse will help to reliably track and condition

234 The floxed C3aR knock-in mouse will help to reliably track and condition

235 Herein, we generated a knock-in mouse with a targeted mutation in the immunorec

236 d tandem-dye Tomato (tdTomato)-C3aR reporter knock-in mouse, which we used to monitor C3aR expression

237 alpha loss-of-function phenotype of the C43S knock-in mouse.

238 pileptic encephalopathy, the Gabrg2(+/Q390X) knock-in mouse.

239 he generation of a floxed GFP-C5aR1 reporter knock-in mouse.

240 he circadian clock using PERIOD2 (PER2)::LUC knock-in mouse.

241 activity were normal in the whole lung of a "knock-in" mouse model carrying an S32T mutation in the P

242 hea from MYPT1(SM+/+) , MYPT1(SM-/-) and the knock-in mutant mice containing nonphosphorylatable MYPT

243 we generated mice with an editing-deficient knock-in mutation (Adar1(E861A), where E861A denotes Glu

244 This knock-in mutation also impairs DNA repair, increases gen

245 was preferentially utilized even though the knock-in mutation could theoretically have been converte

246 Consistent with those findings, mice with a knock-in mutation encoding IFIH1(T946) displayed enhance

247 ding in mouse embryo fibroblasts (MEFs) by a knock-in mutation in Cul9 (Deltap53) increases S-phase c

248 Mice with an inactive knock-in mutation in the p110delta gene (p110delta(D910A

249 A knock-in mutation of the ATM phosphorylation site on E2F

250 of zinc finger function by a C36S/C42S Egln1 knock-in mutation results in upregulation of the erythro

251 The conditional knockout of MYPT1 or the knock-in mutation T853A in mice had no effect on muscari

252 ass) similar to mice globally expressing the knock-in mutation, consistent with the paradigm of innat

253 g a knock-out mutation and the other a L444P knock-in mutation.

254 crine resistance, demonstrate the utility of knock-in mutational models for investigating alternative

255 s in Bap1, including two models in which the knock-in mutations are identical to those reported in hu

256 mice was successfully generated with precise knock-in mutations to incapacitate NLS function.

257 ected immortalized heterozygous R349P desmin knock-in myoblasts to magnetic tweezer experiments that

258 characterized a new mouse model containing a knock-in nonsense mutation (p.R255X) in the Mecp2 locus

259 phorylation by S6K in vitro, through genetic knock-in of a serine-to-alanine mutation (A302).

260 Targeted knock-in of catalytically inactive SUV39H1 in wild-type

261 se activity either by chemical inhibition or knock-in of demethylase-dead UTX resulted in defective m

262 ental transcription factor genes; (3) simple knock-in of epitope tags and fluorescent reporters (e.g.

263 diomyocyte-specific deletion of hepcidin, or knock-in of hepcidin-resistant ferroportin.

264 hanges in metabolism following a single copy knock-in of mutant PIK3CA (H1047R) in the MCF10A cell li

265 We generated a Runx1 P1 knock-in of RUNX1B, termed P1-MRIPV This mouse line lack

266 anded oligo DNA nucleotide (ssODN), allowing knock-in of specific mutations.

267 fusion of an anti-BDNF nAb or in mice with a knock-in of the BDNF Val66Met allele, which blocks the p

268 -Cas9 genome editing to make a single allele knock-in of the most commonly mutated amino acid residue

269 Cells with Nlk deletion or knock-in of the Raptor S863 phosphorylation mutants are

270 in-B3 mutant mice are rescued by conditional knock-in of wild-type ephrin-B3 during the critical peri

271 t require either insertion of exogenous DNA (knock-in) or replacement of genomic segments is very poo

272 We found a lower generation of DYT1 knock-in/p58 knock-out mice than expected from this cros

273 levant model systems with defined natural or knock-in PIK3CA mutations and/or PI3K hyperactivation, w

274 P1 knock-in premegakaryocyte/erythroid progenitors demonstr

275 n, proapoptotic pathways are activated in P1 knock-in premegakaryocyte/erythroid progenitors, presuma

276 the pathological expression profile in Q175 knock-in (Q175 KI) vs Q25 WT mouse models.

277 using CRISPR/Cas9 and produced two lines of knock-in rabbits (rbRosa26-EGFP, and rbRosa26-Cre-report

278 d have achieved multifold improvement on the knock-in rates as well.

279 ted on a screening platform with a T-2A-EGFP knock-in reporter engineered by CRISPR/Cas9.

280 A knock-in reporter for the mouse miR-290 cluster is expre

281 on of wild-type skin grafted onto Fgfbp1 GFP-knock-in reporter hosts and bone marrow transplants from

282 herefore, this study was aimed to generate a knock-in reporter human iPS cell line for MYF5, as an ea

283 ovides valuable guidelines for generation of knock-in reporter human iPS cell lines for myogenic gene

284 cytometry as well as the use of Cldn14-lacZ knock-in reporter mice confirmed increased Cldn14 expres

285 By using a knock-in reporter mouse line expressing tagged SUMO1, we

286 Here, we describe a Dlk1 knock-in reporter mouse that enables single-cell visuali

287 ity of mouse models used in research involve knock-in (reporters or recombinases) or gene replacement

288 In this study, we used mice bearing a knock-in sarcomeric mutation, which is exhibited in huma

289 We have used a knock-out/knock-in strategy in Drosophila to generate a strain wit

290 ntidepressants at SERT have been ablated via knock-in substitution (SERT Met172) without disrupting 5

291 Homologous recombination was used to knock in the most common GBE1 mutation p.Y329S c.986A >

292 in which a tag for affinity purification was knocked in the gene coding for PAG to determine the comp

293 ds, as well as the viability of knockout and knock-in transformants.

294 the human disease, our data demonstrate that knock-in transgenic mice on the NOD background can test

295 cRn) tissue expression in Tg32, a human FcRn knock-in transgenic mouse model, for which a strong corr

296 A CRISPR/Cas9-mediated GFP knock-in visualized the temporal regulation of endogenou

297 mosome (BAC) recombineering and a transgenic knock-in, we have generated a spatiotemporal expressiona

298 e in which the alkaline phosphatase gene was knocked-in, we studied the general effects after gene re

299 To test this hypothesis, we crossed DYT1 knock-in with p58(IPK)-null mice.

300 with an ERalpha DNA-binding domain mutation knocked in (WT/KI) to produce WT, ERalpha KO, or ERalpha