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   1 nsitive phenotype of the Arabidopsis mtp11-3 knockout mutant.                                        
     2 ein import-defective phenotypes of an hsp93V knockout mutant.                                        
     3 faced core gp120 probe and its cognate CD4bs knockout mutant.                                        
     4 revealed that this strain is not a true prrA knockout mutant.                                        
     5 al surface of the wild type but not the OppA knockout mutant.                                        
     6 plete absence of tassel branches in the bif2 knockout mutant.                                        
     7 in five times faster than into the fcbT1T2T3 knockout mutant.                                        
     8 ignaling might be affected in the Lpp double-knockout mutant.                                        
     9 n system were not affected in the lpp double-knockout mutant.                                        
    10 upernatant of cells infected with a cmvIL-10-knockout mutant.                                        
    11 s and T84 cells infected with the lpp double-knockout mutant.                                        
    12 e susceptible to infection by the lpp double-knockout mutant.                                        
    13 mitochondrial calcium uptake capacity in the knockout mutant.                                        
    14 opied an ACTIN DEPOLYMERIZING FACTOR4 (ADF4) knockout mutant.                                        
    15  Physcomitrella patens, we generated a pgrl1 knockout mutant.                                        
    16 and root epidermal protoplasts of the Atann1 knockout mutant.                                        
    17 oth responses are largely diminished in shyg knockout mutants.                                       
    18  were determined via SMRT sequencing of gene knockout mutants.                                       
    19  core proteins and their corresponding CD4bs knockout mutants.                                       
    20 pendent of target size, exclusively recovers knockout mutants.                                       
    21 y particle bombardment to engineer 1201 gene knockout mutants.                                       
    22 ploid-convertible heterozygous diploid yeast knockout mutants.                                       
    23  and XI-2/XI-B, using single and double gene-knockout mutants.                                       
    24 o molybdoenzymes in Arabidopsis thaliana PCD knockout mutants.                                       
    25 were studied by growth phenotype analysis of knockout mutants.                                       
    26 absence of LIP8 expression in the homozygous knockout mutants.                                       
    27 athogenicity island (PAI) mutants, or double knockout mutants.                                       
    28 ignal transduction pathway is present in the knockout mutants.                                       
    29 was slower than that of wild type and single knockout mutants.                                       
    30 he phenotype of Fz5/Fz8 compound conditional knockout mutants.                                       
    31 an explain some phenotypes observed in ife-4 knockout mutants.                                       
    32 nalyzed by using Tn5 transposon-mediated spu knockout mutants.                                       
    33 mizygous Bax +/- controls, and three Bax -/- knockout mutants.                                       
    34 hemizygous Bax +/- controls, and six Bax -/- knockout mutants.                                       
    35 p in resistance, we created double NspA/FHbp knockout mutants.                                       
    36 tor degeneration than that in the RPGR null (knockout) mutant.                                       
    37   Expression of VviCCC in an Arabidopsis ccc knockout mutant abolished the mutant's stunted growth ph
    38 tein accumulation by systematically stacking knockout mutant alleles of all four members (alphaVPE, b
  
    40 gregation and molecular analyses of the NPG1 knockout mutant and a cross with a male sterile mutant i
    41 lso observed in a DeltapauA2DeltaphoU double knockout mutant and complemented by the wild-type phoU g
    42 organisms in acid was absent in the alpha-CA knockout mutant and in the presence of acetazolamide, al
  
    44 ng molecular and biological responses of the knockout mutant and overexpression lines of AtRAP upon b
  
  
  
    48 ological studies on Drosophila atm and mre11 knockout mutants and discovered a telomere defect that w
    49  PLATZ1, PLATZ2, and AGL67 were confirmed by knockout mutants and overexpression in a desiccation-int
    50 udy system, we employ reverse genetic tools (knockout mutants and RNA interference) to demonstrate th
  
  
    53 d transporter1 and ferric chelate reductase2 knockout mutants and was prioritized over immunity, as h
    54 ies using gp120 core protein, single-residue knockout mutants, and chimeric viruses revealed that G37
    55 n, we generated dcl2drb4 and dcl4drb4 double knockout mutants, and subjected them to infections with 
  
    57 ae and that several single and multiple gene-knockout mutants are computationally predicted to improv
  
  
  
  
  
    63 emarkably, in some plant species, homozygous knockout mutants can be produced in a single generation.
  
    65 iae yeast strain, and Mn sensitivity of mtp8 knockout mutants characterized the protein as a vacuolar
    66     Leaf senescence is delayed in the SAG113 knockout mutant compared with that in the wild type, sto
    67 sed significantly by over 30% in the Bax -/- knockout mutants compared with wild type and hemizygote 
  
  
    70 tenoid hydroxylase activities in vivo, T-DNA knockout mutants corresponding to beta-hydroxylases 1 an
  
  
    73 tored in the wild-type strain, as well as in knockout mutants defective in a variety of methylotrophy
    74 accine strain measles virus (MV) or isogenic knockout mutants deficient for either V (V(ko)) or C (C(
    75 -rbt1), its control (DAY286), homozygous rbt knockout mutants deficient in rbt1 (BCa7-4) or rbt4 (BCa
    76 t mutant, DeltaaebG V. harveyi, and the NRPS knockout mutant, DeltaaebF V. harveyi, do not produce am
  
  
    79 The construction of nonpolar markerless phpP knockout mutants (DeltaphpP) in two pathogenic strains, 
    80 n from Taiwan, together with an isogenic PVL-knockout mutant (Deltapvl) and complemented PVL-positive
    81  wild-type S. aureus cell but not the double knockout mutant DeltatarM/S, which lacks both alpha- and
    82 ve cell-wall analytical assays of the double knockout mutant demonstrated reduced levels of pectin me
    83 t construction and examination of single ccm knockout mutants demonstrated that membrane insertion an
  
  
  
    87 e role of LjNPF8.6 on nodule functioning, as knockout mutants display N-fixation deficiency (25%) and
  
    89  Consistently with this observation, an npr3 knockout mutant displayed enhanced resistance to Pseudom
  
  
  
  
    94 xpressing plants stayed green and the sgr2-1 knockout mutant exhibited early leaf yellowing under age
    95 not observed in slice cultures from Munc13-1 knockout mutants exhibiting defective vesicle priming.  
  
  
    98  that of wild-type A. hydrophila; the triple-knockout mutant failed to induce any detectable level of
  
   100  Physiological analysis of a transferred DNA knockout mutant for AtALMT1 as well as electrophysiologi
  
   102    This indicates the possible usage of TTM2 knockout mutants for agricultural applications to genera
  
  
  
  
   107 gene switch status, as well as isogenic gene knockout mutants for cagE, oipA, babA2, hopZ, cagE/oipA,
  
   109 o, we conducted a comparative study of T-DNA knockout mutants for each Tic gene, and for the most abu
  
   111  of Arabidopsis, including single and double knockout mutants for the high-affinity transporter, AtHA
   112 d cytokinin degradation as well as different knockout mutants for the negative AUX/IAA regulators shy
   113 lished a comprehensive study covering 269 TF knockout mutants for the yeast Saccharomyces cerevisiae.
   114  of lignified tissues of various Arabidopsis knockout mutants (for AtCAD5, 6, and 9) at different sta
  
  
  
  
  
   120 nd glucan binding protein C (GbpC) in a tarC knockout mutant (GMS900), thereby supporting the notion 
  
  
  
  
  
  
  
   128 fer is specific to the donor strain; an lpqM knockout mutant in the recipient is still proficient in 
  
  
  
  
  
  
   135 -Bar_gosGFP to produce activation-tagged and knockout mutants in the processing tomato cultivar M82. 
   136 lmonella enterica serovar Typhimurium double-knockout mutants in which either the lipoprotein A (lppA
   137 y, we describe a method for rapidly creating knockout mutants in which we make use of yeast recombina
  
  
  
   141 , the resulting nonfeasibility of creating a knockout mutant is a major limiting factor in studying i
  
  
   144 igin of replication and the M. smegmatis glf knockout mutant is unable to grow at the higher temperat
   145 wever, a striking characteristic of elt-2(0) knockout mutants is that while they die shortly after ha
   146 rate that the backcrossing of wild mice with knockout mutant laboratory mice retrieves behavioural tr
  
  
  
   150  and early seedling development, we utilized knockout mutants lacking one or more of these components
  
  
  
  
   155     Chloroplasts isolated from the atHsp93-V knockout mutant line are still able to import a variety 
   156 cal, and pollen germination studies with the knockout mutant line indicate that NPG1 is not necessary
   157 owth conditions, two independent Arabidopsis knockout mutant lines displayed significantly reduced le
  
  
   160 rotein import into chloroplasts, we isolated knockout mutant lines that contain T-DNA disruptions in 
  
  
  
   164 types to those found in the respective Kcnq2 knockout mutant mice, suggesting that Kcnq2 haploinsuffi
  
  
  
  
  
  
   171 uppressor screen of a transfer DNA insertion knockout mutant of AtCNGC2 in order to identify downstre
  
  
  
  
   176 ar preparations of an isogenic lst insertion knockout mutant of N. gonorrhoeae F62 (strain ST01) expr
   177 nti-VT2 antibodies, while a VT1 and VT2 gene knockout mutant of PM601 was unable to stimulate JNK act
   178 M NaCl treatment in wild-type rice (WT); GR3 knockout mutant of rice (gr3); and the functional gr3-co
   179 lonized more efficiently on the sgc protease knockout mutant of S. gordonii than on the parent biofil
   180 uced by transfection of a nonreplicative NS1 knockout mutant of the MVC infectious clone, as well as 
   181 t findings of embryo death in the homozygous knockout mutant of the plastid LPAAT contrasts with earl
   182  to the identification of a grazer-resistant knockout mutant of the wzm ABC O-antigen transporter gen
  
  
  
   186  next isolated and characterized insertional knockout mutants of all three isoforms confirming a comp
  
  
  
  
  
  
  
   194 , pollen fitness, and pollen tube growth for knockout mutants of five of the six myosin XI genes expr
  
  
   197 e analyses of overexpressing hairy roots and knockout mutants of MtMYB5 and MtMYB14 indicate that MtM
  
  
   200 o indicated by frequently subdued effects of knockout mutants of regulators, their evolutionary losse
   201 fter 24 h of focal cerebral ischemia in both knockout mutants of SOD1 (Sod1 -/+ and Sod1 -/-) and wil
   202      In this study, we attempted to generate knockout mutants of the ortholog of yeast HOG1 MAP kinas
  
  
   205 sition system to generate single- and double-knockout mutants of two tandemly duplicated cytochrome P
  
   207 ve heat shock response of mycobacterial sigE knockout mutants only in the presence of a functional my
  
  
  
  
  
  
  
   215  overexpress BEE1 with bee1 bee2 bee3 triple-knockout mutant plants suggests that BEE1, BEE2, and BEE
   216 fication and characterization of two atToc34 knockout mutants, plastid protein import 3-1 (ppi3-1) an
   217 s (Arabidopsis thaliana) and phospholipase D knockout mutants pld zeta1, pld zeta2, and pld zeta1 pld
   218 n FH domains 6 and 7 fused to Fc than double knockout mutants prepared from two sensitive meningococc
  
  
   221 on is abrogated in double and quadruple pp2c knockout mutants, provoking, similarly to SnRK1 overexpr
   222 in mice of 2,578 barcoded Plasmodium berghei knockout mutants, representing >50% of the genome, and c
   223  SXD1 in a Synechocystis sp. PCC6803 slr1737 knockout mutant restored tocopherol synthesis, indicatin
  
   225 ariants produced by a M. tuberculosis Rv2181 knockout mutant revealed the presence of but a single Ma
   226 ual module knockout mutant and single module knockout mutants revealed that 4-nitrotryptophan is an i
  
   228 nerated the recombinant rGLS virus and an NS knockout mutant, rGLSNSdelta virus, using reverse geneti
  
   230 lowed Arabidopsis wild type but not AtHSP101 knockout mutant seedlings to survive otherwise lethal te
   231 s transposition in calli derived from dng701 knockout mutant seeds compared with that in wild-type ca
  
  
  
  
  
  
  
   239  in different organs of wild-type and a ggt3 knockout mutant showed that GGT3 was a major contributor
  
   241 lysaccharide preparations from RGP1 and RGP2 knockout mutants showed a significant reduction in total
  
  
  
  
   246 ota/1145/2007 (H3N2) (SIV 1145-WT), a PB1-F2 knockout mutant (SIV 1145-KO), and its N66S variant (SIV
  
   248      Plate-killing assays indicate that a PE-knockout mutant strain of Escherichia coli drastically o
  
  
  
  
   253 RNA blots and analysis of enzyme activity in knockout mutants suggest that GGT1 is expressed most str
  
  
  
   257  and actin-interacting protein 1, as well as knockout mutants that lack Coronin and actin-interacting
  
  
  
  
  
   263     The increased sensitivity of atpollambda knockout mutants toward high salinity and MMC treatments
  
   265 ative analysis of seedling growth of snrk2.4 knockout mutants versus wild-type Arabidopsis suggests t
   266 ally induced apoptosis indicated that the NS knockout mutant virus induces earlier and increased DNA 
   267 Compared to the wild-type virus, the ToV-PLP knockout mutant virus showed impaired growth and induced
   268 tosis indicated that the NV-deficient and NV knockout mutant viruses induce apoptosis earlier in cell
   269 ed earlier, amylovoran production in an amyR knockout mutant was about eight-fold higher than that in
   270 t Dicarboxylate Transporter (tDT) in the tdt knockout mutant was associated previously with an impair
  
  
  
  
  
   276 role of LytSR in biofilm development, a lytS knockout mutant was generated from a clinical S. aureus 
  
   278 t in reduced virulence; however, an mltE(EA) knockout mutant was reduced in virulence and growth in i
  
   280 rimary roots in PLDzeta1 and PLDzeta2 double knockout mutants was slower than that of wild type and s
   281 y comparative metabolomics of overexpression/knockout mutants, we identified a tryptophan-derived iro
   282 ugh construction and analysis of a series of knockout mutants, we identified the genes necessary for 
  
  
  
  
  
  
  
   290 lated in As-treated Arabidopsis, and ggct2;1 knockout mutants were more tolerant to As and cadmium th
  
   292 ssing the ppi3 mutants with ppi1, an atToc33 knockout mutant) were unsuccessful, indicating that the 
  
  
   295 h the investigation of independent complex I knockout mutants, which were found to have corresponding
   296 t, and (iii) act and ast genes; and a triple-knockout mutant with truncation in all three genes, act,
   297  the number of Purkinje and granule cells in knockout mutants with a deletion in the proapoptotic gen
  
   299 e markedly opposite phenotypes to the double knockout mutant, with increased cell-wall methylesterifi
  
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