ライフサイエンスコーパス: knottin

1 in precursor, an arrangement common to other knottins.

2 mice by small animal CT, and both 64Cu-DOTA-knottin 2.5F and FDG were able to differentiate lung nod

3 Collectively, these results show 64Cu-DOTA-knottin 2.5F to be a promising candidate for clinical tr

4 Ex vivo biodistribution showed 64Cu-DOTA-knottin 2.5F to have a fast renal clearance combined wit

5 Uptake and retention of the 64Cu-DOTA-knottin 2.5F tracer in the lung tumors combined with a l

6 We thereby isolated variant knottins against cellulose (differing in sequence from t

7 ulose (differing in sequence from the parent knottin) and also against alkaline phosphatase.

8 Knottins are a group of small, disulphide-bonded protein

9 Similar to other knottins, cystine knot alpha-amylase inhibitors are high

10 The best oxime-conjugated knottin dimer achieved an unprecedented 150-fold increas

11 based dimerization strategy was critical, as knottin dimers created through genetic fusion to a bival

12 cross-linkers of different lengths to create knottin dimers with varying molecular topologies.

13 o contains a region homologous to the TAXI-1 knottin domain; however, a deletion in this domain restr

14 d by dedicated genes or as a domain within a knottin-encoding PA1-albumin-like gene.

15 t the so-called "inhibitor cystine knot" or "knottin" fold stabilized by three disulfide bonds.

16 share the CCK motif with trypsin-inhibitory knottins from a plant in the Cucurbitaceae family.

17 The binding of MB-Knottin(Integrin) and MB-Knottin(Scrambled) to alpha(v)b

18 groups of tumor-bearing mice imaged with MB-Knottin(Integrin) and with MB(cRGD).

19 MB-Knottin(Integrin) attached significantly more to alpha(v

20 maging signal after the administration of MB-Knottin(Integrin) decreased significantly (by 64%).

21 cantly higher after the administration of MB-Knottin(Integrin) than after the administration of MB(al

22 blocking of integrins, the attachment of MB-Knottin(Integrin) to alpha(v)beta(3) integrin-positive c

23 ging contrast agent was created by attaching Knottin(Integrin) to the shell of perfluorocarbon-filled

24 aging signals after the administration of MB-Knottin(Integrin) were not significantly different in th

25 (3) integrins with a low nanomolar affinity (Knottin(Integrin)).

26 l of perfluorocarbon-filled microbubbles (MB-Knottin(Integrin)).

27 egrin-positive cells (0.15 +/- 0.12) than MB-Knottin(Integrin).

28 newly detected homologs, particularly among knottin-like domains.

29 ize dimers of integrin-binding cystine knot (knottin) miniproteins with low-picomolar binding affinit

30 s introduced at different locations within a knottin monomer and reacted with dialdehyde-containing c

31 crease in apparent binding affinity over the knottin monomer.

32 -fold) in tumor cell binding relative to the knottin monomer.

33 t forms the inhibitory cystine knot (ICK) or knottin motif.

34 e/gram (%ID/g), compared with a low-affinity knottin peptide (IC(50), approximately 0.4 mumol/L; 1.48

35 The imaging results obtained with the knottin peptide are compared with standard 18F-fluorodeo

36 decane-N,N',N'',N'''-tetraacetic acid (DOTA)-knottin peptide that targets integrins upregulated durin

37 A knottin peptide with a scrambled sequence was used to cr

38 This integrin-binding knottin peptide, denoted EETI 2.5F, was evaluated as a m

39 We report a knottin peptide-drug conjugate (KDC) and demonstrate tha

40 with a fluorescent, engineered cystine knot (knottin) peptide that binds with high affinity to alphav

41 a small, disulfide-constrained cystine knot (knottin) peptide that bound to alpha(v)beta(3) integrins

42 pared with other engineered integrin-binding knottin peptides and with c(RGDfK), a well-studied integ

43 Integrin-binding knottin peptides can be conjugated to the surface of mic

44 Furthermore, (64)Cu-DOTA-conjugated knottin peptides generated lower levels of nonspecific l

45 Thus, engineered integrin-binding knottin peptides show great potential as clinical diagno

46 usly, we used directed evolution to engineer knottin peptides that bind with high affinity ( approxim

47 is work expands the therapeutic relevance of knottin peptides to include targeted drug delivery, and

48 roximately 20 nmol/L) (64)Cu-DOTA-conjugated knottin peptides was 4.47% +/- 1.21% and 4.56% +/- 0.64%

49 (64)Cu-DOTA-conjugated knottin peptides were stable in mouse serum, and in vivo

50 y plays a strong role in the tumor uptake of knottin peptides.

51 3.0 A) and the antifungal protein Rs-Afp1 [a knottin protein from radish (Raphanus sativus), rmsd of

52 bility to inhibit binding of a biotinylated "knottin"-RGD peptide to surface-immobilized integrins an

53 ers, such as integrin-immobilization levels, knottin-RGD concentration, buffer compositions, type of

54 near GRGDS, (ii) cyclo[RGDfK], and (iii) the knottin-RGD itself for binding to three different integr

55 We introduced the biotinylated knottin-RGD peptide instead of biotinylated cyclo[RGDfK]

56 Binding of knottin-RGD peptide was strongest for alphavbeta3 but al

57 Knottin scaffolds therefore appear to be a promising arc

58 Control MB-Knottin(Scrambled) adhered less to alpha(v)beta(3) integ

59 The binding of MB-Knottin(Integrin) and MB-Knottin(Scrambled) to alpha(v)beta(3) integrin-positive

60 was used to create control microbubbles (MB-Knottin(Scrambled)).

61 inistration of MB(alphavbeta3) or control MB-Knottin(Scrambled).

62 Here, we attempted to create knottins with novel binding activities based on the cell