ライフサイエンスコーパス: labeling

1 D-CC, while offering the advantages of (18)F labeling.

2 rated previously by Balaban through isotopic labeling.

3 he hyperalgesic priming model, we used c-fos labeling.

4 in the electron microscope without specific labeling.

5 ed unambiguously by (13)C and (15)N isotopic labeling.

6 e reporter protein hence the sparse neuronal labeling.

7 an anti-PD-L1 adnectin after (18)F-fluorine labeling.

8 a similar stereotyped branching pattern DiI-labeling.

9 NOAC doses are often inconsistent with drug labeling.

10 vent (hexane versus toluene) and to isotopic labeling.

11 dase and GABA with post-embedding immunogold labeling.

12 idopsis mutant atg5 correlated with N-BODIPY labeling.

13 n rates directly without a proxy or isotopic labeling.

14 athway from the BC by dual-color anterograde labeling.

15 igments in coatings, cosmetics, and security labeling.

16 t molecular specificity through fluorescence labeling.

17 quantification approach based on aminoxyTMT labeling.

18 combination of mouse genetics and retrograde labeling.

19 aforementioned structures with (13)C-isotope labeling.

20 nd auditory cortex did not reveal any double labeling.

21 s been applied to bioconjugation and peptide labeling.

22 rotein synthesis, as determined by metabolic labeling.

23 ssels and salivary glands contained tdTomato labeling.

24 is modified proportionally to the metabolite labeling.

25 se core granules was confirmed by immunogold labeling.

26 oup reported ignoring precautionary allergen labeling.

27 on isothermal amplification and fluorescence labeling.

28 s and to perfusion images from arterial spin labeling.

29 )Mn, through both remote-loading and surface labeling.

30 h monoallelic green fluorescent protein tags labeling 10 proteins representing major cellular structu

31 ntramolecular side reactions during chemical labeling, allowing efficient chemical tagging with a red

32 Labeling along the antennal nerve, in projections of the

33 e visualized with FITC conjugated BSI-lectin labeling and arteriole diameters were compared before an

34 at this approach is generally applicable for labeling and detection of suitable glycosaminoglycans.

35 racking methods based on thymine analog cell labeling and developed tailored mathematical models to q

36 cytoplasmic surface using site-directed spin labeling and double electron-electron resonance spectros

37 acoustic imaging assisted by smart molecular labeling and dynamic contrast enhancement approaches tha

38 Current interest has focused on how labeling and education influence adherence.

39 Using retrograde labeling and electrophysiological techniques combined wi

40 gh temporal resolution requires high-density labeling and extraordinary fluorophore photostability.

41 escence-based assay, together with substrate labeling and kinetics-based approaches, complementary ex

42 d doxycycline), we demonstrate that covalent labeling and mass spectrometry (MS) can be used to map s

43 t can now be used to conduct various protein labeling and modifications that were extremely challengi

44 Overall, this system allows stable labeling and monitoring of HIV genomic DNA within infect

45 ineering in mice enables the time-controlled labeling and monitoring of lipogenic or myogenic populat

46 e pathologist interpretations for both image-labeling and nuclei-labeling tasks (83% and 87%), as com

47 Proximity labeling and protein interaction network analysis reveal

48 r concentration (0.7 nmol) further increased labeling and quantitative yields to more than 98% within

49 Deuterium-labeling and stoichiometric experiments support a mechan

50 able more accurate measurement of metabolite labeling and thus metabolic flux.

51 We have used isotope labeling and two-dimensional infrared spectroscopy to sp

52 Methods based on site-specific labeling and ultrafast spectroscopic detection of fluore

53 substrate for selective radio-frequency (RF) labeling and, (ii) the process of immobilisation upon bi

54 Using a combination of MS, isotope labeling, and (1)H and (13)C NMR techniques, we establis

55 ton Ca(2+) imaging, triple immunofluorescent labeling, and 3D electron microscopic (EM) reconstructio

56 By combining in situ gas analysis, isotopic labeling, and a surface carbonate titration, we show tha

57 ive to the methods of conjugation, degree of labeling, and contamination with free dyes.

58 been made with intact methods for clearing, labeling, and imaging whole organs such as the mature br

59 round, a red split-sfCherry2 for multicolour labeling, and its photoactivatable variant for super-res

60 Analysis by immunoblotting, metabolic labeling, and mass spectrometry demonstrated that the ma

61 mbination of in situ spectroscopic, isotopic labeling, and mass spectroscopic investigations.

62 e preparation (i.e., mutations, paramagnetic labeling, and reconstitution in lipid bilayers) for both

63 th the sample, such as dilution, separation, labeling, and use of special cuvettes.

64 alternative systems for both single- or dual-labeling applications.

65 Chromophore quench-labeling applied to 1-octene polymerization as catalyzed

66 A clear advantage of the sparse labeling approach is demonstrated for the cytosolic muta

67 We report a proximity-based labeling approach that uses an antibody to a target anti

68 Applying this labeling approach to structured illumination microscopy

69 We expect that this combined covalent labeling approach will be applicable to other protein/sm

70 Using a functional obstructive labeling approach, we show that activation of BAK involv

71 have been possible without using the reverse labeling approach.

72 Using multiple labeling approaches to identify pathways and their posts

73 rapid room-temperature two-pot procedure for labeling arylcarboxylic acids and amide derivatives with

74 magnetic resonance scans using arterial spin labeling (ASL) were performed to study the haemodynamic

75 yl transferase-mediated dUTP-biotin nick end labeling assay and Hoechst staining.

76 cleotidyl transferase-mediated dUTP nick-end labeling assay was performed, and intestinal inflammatio

77 luated the sensitivity and specificity of EV labeling assays in a manner that has not been described

78 Metabolic labeling assays showed that targeting these three 3'-UTR

79 ncludes abundant protein depletion, isobaric labeling at the peptide level for multiplexed relative q

80 ent functional groups for post-translational labeling at the specific amino acid positions.

81 e calculated using AAL (Automated Anatomical Labeling atlas) volumes of interest (VOIs) for parietal,

82 Using isobaric labeling-based quantitative proteomics, we simultaneousl

83 rnative to fluorescent probes for biological labeling because of their photostability and multiplexin

84 Stable isotope labeling by amino acids in cell culture, using primary c

85 stion and a possibility for further chemical labeling by introducing reactive spirolactone moieties.

86 The main advantage of only labeling C3 and C4 positions is the absence of (13)C-(13

87 work, a novel strategy integrating isotopic labeling, chemical enrichment and multiplexed proteomics

88 The diDO-IPTL labeling chemistry uses only high-purity, relatively ine

89 -based tracer concept was adapted to (99m)Tc-labeling chemistry.

90 d based on high-performance chemical isotope labeling (CIL) nanoflow liquid chromatography mass spect

91 r-resolution images show that independent of labeling conditions and cell type 50-60 % of all membran

92 ells eliminated lactate-dependent metabolite labeling, confirming tumor-cell-autonomous lactate uptak

93 ed circular dichroism and site-directed spin labeling coupled with electron paramagnetic resonance to

94 tural isotope correction for high-resolution labeling data requires more sophisticated algorithms tha

95 Nuclear labeling decreased dramatically after infection, accompa

96 luoric acid resistance, (31)P-NMR, and (32)P labeling demonstrated the lack of phosphodiester bonds,

97 By labeling different particle sizes with dyes/QDs for LCN1

98 ybridized with sequence specific capture and labeling DNA probes in solution and then the complex is

99 We show that QD labeling does not affect major biophysical properties of

100 The mdDiLeu labeling does not suffer from precursor interference and

101 entified by sequencing DNA barcodes uniquely labeling each of the tested cytokines.

102 sparse labeling of the protein (reducing the labeling efficiency f) and reducing the excitation proba

103 iation in tagged-ZnT8 expression and surface labeling enabled sorting of heterogeneous beta cells to

104 sing SPARCLE: Subfamily Protein Architecture Labeling Engine.

105 (Glu) and glutamine (Gln) and arterial spin labeling evaluation for rCBF.

106 d in vivo using a pulse-chase stable isotope labeling experiment.

107 We carried out metabolic labeling experiments and studies of mice with human-like

108 Cell-surface labeling experiments indicate that the dominant form of

109 Previous dye-labeling experiments showed that a single CE cell could

110 -Iododeoxyuridine/5-chlorodeoxyuridine pulse-labeling experiments showed that RAD6 is necessary for o

111 Kinetic study with extensive deuterium labeling experiments were performed to support the propo

112 Energy-resolved mass spectrometry, deuterium labeling experiments, and theoretical calculations (B3LY

113 The kinetics data, coupled with deuterium-labeling experiments, support a mechanism involving diss

114 nger ligations, setting the stage for tandem labeling experiments.

115 R, UV-vis spectroscopy, and isotopic ((18)O) labeling experiments.

116 Moreover, utilizing the TC-labeling FlAsH-EDT2 biarsenical reagent to visualize VFs

117 The results demonstrated that covalent labeling followed by MS markedly increased the predictiv

118 sing light and electron microscopy, we found labeling for all three Nav subtypes on dendrites, dendri

119 etric analysis of basophil responses implied labeling for CD63, CD203c, and intracellular histamine.

120 Here we use bio-orthogonal proteome labeling for detecting in vivo proteins present only in

121 but the proportion of pre- and post-synaptic labeling for each subtype varied within and between subr

122 Immunogold labeling for GABA confirmed that the transplanted cells

123 require either genetic or exogenous contrast labeling for light microscopy.

124 and ionization, and utilizes stable isotope labeling for MS1 level identification of hydrophobic pep

125 demonstrated by Gclc targeted single-neuron labeling from the inducible Cre-mediated knockout (SLICK

126 lthy individuals (n=23) during arterial spin labeling functional magnetic resonance imaging (fMRI).

127 We also survey the methodologies for labeling GPCRs with biophysical probes, particularly flu

128 which can be distinguished through isotopic labeling, have not been considered in past syntheses.

129 o the online ordering system (targeting menu labeling, healthy food availability, placement, and prom

130 eful in rapid carboxylic acid carbon isotope labeling, however development toward its application as

131 gami nanostructures to quantitatively assess labeling, imaging, and washing efficiency.

132 quantitative polymerase chain reaction, dual-labeling immunofluorescent staining, and immunoassays.

133 This study used multiple labeling immunohistochemistry, confocal maging and analy

134 beling was observed in 36% and exclusive YFP labeling in 9%.

135 ative gel electrophoresis and stable isotope labeling in cell culture proteomics that the TbSTT3A and

136 Here we present Breaks Labeling In Situ and Sequencing (BLISS), featuring the f

137 The retention of GFP labeling in slowly dividing cells allowed for localizati

138 The isotopic labeling increases the vibrational lifetime about 2-fold

139 tion of the two results in an attenuation of labeling, indicating a shift toward outward-facing confo

140 Nutrition labeling is a prominent policy to promote healthy eating

141 Our study shows that (15)N and (18)O isotope labeling is a useful approach for direct quantification

142 Specific labeling is achieved by presenting a tethered ligand at

143 spectrum of the substance in which the (13)C labeling is known a priori.

144 The combined effects of continuous labeling (label pumping), dynamic reversible binding, an

145 These results show that (12)C-/(13)C-DnsHz labeling LC-MS is a useful tool for profiling the carbon

146 ells after gemcitabine treatment using iTRAQ labeling LC-MS/MS, because it was featured with the adva

147 However, when labeling live cells to monitor surface biomarker express

148 The methodology combines covalent labeling, mass spectrometry, and kinetic analyses in a s

149 dimensions simultaneously by using proximity labeling mediated by engineered ascorbic acid peroxidase

150 rein, we demonstrated that the fluorescamine labeling method developed by our group not only could re

151 article provides an overview of recent (18)F-labeling methodologies, specifically for B-(18)F, Si-(18

152 available for most organisms and alternative labeling methods are very limited.

153 Although advances in protein labeling methods have made it possible to measure the pr

154 Metabolic and bioorthogonal labeling methods have previously enabled the enrichment

155 However, each site-specific labeling modification might affect rates of folding of n

156 cadre of new and powerful tools for in vivo labeling, monitoring, and manipulation of neurons were a

157 Here we use isobaric-labeling multiplexed proteomics to analyze protein co-re

158 The aim of this work was to optimize labeling of (68)Ga-PSMA(HBED) using the efficient cation

159 Hence, exploiting the (15)N labeling of a cellular sample accelerates subsequent ana

160 ted AAT-AMS program was effective in both de-labeling of AALs and promotion of improved antibiotic us

161 Moreover, acridinium-labeling of AtRALF1 indicated that the binding of AtRALF

162 of this conjugation chemistry enables facile labeling of bacteria.

163 mart probes greatly facilitate the selective labeling of biomolecules in living system.

164 Selective labeling of blood monocyte subsets indicates that non-cl

165 Fluorescent labeling of budding yeast nucleoli with CDC14-GFP reveal

166 In vivo labeling of central nervous system proteins with stable

167 We couple isotopic labeling of cyanobacterially derived dissolved organic m

168 ally after infection, accompanied by intense labeling of cytoplasmic foci.

169 Retrograde fluorescent labeling of dental primary afferent neurons (DPANs) has

170 (89)Zr-labeling of Df-ALT-836 was achieved in high yield and go

171 , a cellular pre-extraction protocol and the labeling of DNA-Pt by means of click chemistry in cells.

172 lary tufts by combining in vivo fluorescence labeling of endothelial cells, a novel tissue-clearing t

173 Although labeling of Escherichia coli with the chemical reporter

174 CD44 in breast cancer cells, we demonstrate labeling of fixed cells with high specificity that corre

175 directed nickase system directs the specific labeling of human (TTAGGG)n DNA tracts in genomes that h

176 Therefore, the rate of metabolic (15)N labeling of individual lipid species could be determined

177 Subsequent labeling of intermediate and nonclassical monocytes is c

178 of such probes was demonstrated by selective labeling of lipid and hCAII in Hela cells.

179 Purpose To determine whether endogenous labeling of macrophages with clinically applicable nanop

180 tion nanoscopy, we imaged secondary antibody labeling of monoclonal AQP4-IgGs with differing epitope

181 epresentative aryl boron reagent, and on the labeling of more than 50 (hetero)aryl boronic esters.

182 ugh several markers have been identified for labeling of MSPCs, these labeled cells still contain non

183 s review, we describe recent advances in the labeling of nanomedicines, how imaging studies are guidi

184 etase (L274G) enables the cell-type-specific labeling of nascent proteins with a non-canonical amino-

185 This step was also devised to dual-labeling of PCR products with biotin and 6-FAM, which ar

186 ) but statistically significant reduction in labeling of PD-L1 expression in tumor cells.

187 Double-transgenic mice showed 55% double labeling of periurethral neuroendocrine cells expressing

188 e first time a way for direct, site-specific labeling of proteins with fluorescent-dyes in live cells

189 The labeling of proteins with ubiquitin/ubiquitin-like (Ubl)

190 Labeling of PSMA tracers with (18)F offers numerous adva

191 In particular, the dynamic (13)C-labeling of pyruvate and lactate formed from (13)C-gluco

192 de expansion and site-specific bioorthogonal labeling of rhodopsin with Alexa488 to enable, to our kn

193 Cell-specific labeling of RNA can be profiled and imaged using bioorth

194 f-flight mass spectrometry combined with the labeling of specific ligands with mass tags enables dete

195 thin living cells, with the highly efficient labeling of subcellular components and the activation of

196 tients with (13)C-lactate revealed extensive labeling of TCA cycle metabolites.

197 Control experiments showed no labeling of TG2 knock-out mice.

198 demonstrated through the effect of isotopic labeling of the methylammonium (MA) component on the die

199 tained is through imaging, and this requires labeling of the nanomedicine to enable detection outside

200 ctivation mechanism concluding with covalent labeling of the PLP cofactor.

201 pressing multispin effects in PELDOR, sparse labeling of the protein (reducing the labeling efficienc

202 ctrometry-based proteomics, and the chemical labeling of the resulting peptides is often used for pep

203 mics, transcriptomics, proteomics, and (13)C-labeling of type VI glandular trichomes and leaves from

204 was measured across the brain during gender labeling of varying intensities of angry, happy, or fear

205 Here we use combinatorial labeling of zebrafish cranial neural crest-derived cells

206 ehydes is selectively labeled, and a one-pot labeling/olefination method is also described.

207 By fluorescently labeling only a small fraction of cellular Pil1p, the ma

208 irst direct LC-MS/MS study (without isotopic labeling or hydrolysis) of primary oxidation sites of p5

209 d GalNAz-labeled surface cells, causing DAPI labeling (permeabilization) of underlying cells.

210 Measurements at two independent labeling positions on opposite sides of the ring showed

211 ent is promising as a convenient synthon for labeling potential PET radiotracers.

212 Using solution NMR and a labeling procedure in which a series of mixed proteasome

213 A robust and reliable kit-labeling procedure was established, allowing the prepara

214 ed a pressing need for efficient fluorescent labeling procedures to visualize and detect EVs.

215 d cell-surface analysis without the need for labeling processes.

216 de expansion provides an attractive tool for labeling proteins with a minimal, bright tag in quantita

217 This improved SPM and labeling protocol is an important step toward a useful P

218 Genetic code expansion and bioorthogonal labeling provide for the first time a way for direct, si

219 sis, kinetic isotope effects, stereochemical labeling, radical clock, and transient absorption studie

220 nected to the lymphatic system, with LYVE(+) labeling reaching the lens along the suspensory ligament

221 Labeling reaction conditions (100mM borate buffer at pH

222 n conditions (100mM borate buffer at pH 8.5, labeling reaction time 60min, temperature 70 degrees C a

223 nt chemical tagging with a reduced excess of labeling reagent without intramolecular side reactions.

224 Specifically, three different covalent labeling reagents, namely diethylpyrocarbonate, 2,3-buta

225 barcoding approaches require time intensive labeling, reduce the number of biologically meaningful p

226 showed prominent filamentous actin (F-actin) labeling reflecting cells in a contracted state.

227 and Drug Administration's updated nutrition labeling requirements will include added sugars starting

228 cording to U.S. Food and Drug Administration labeling requirements.

229 The labeling results reveal binding sites that are consisten

230 Immunogold labeling revealed abundant alphaS intimately associated

231 ions mainly through layer 2b cells, and dual-labeling revealed many cells extending collaterals to bo

232 ytometry analysis after fluorescent antibody labeling revealed strong correlations among the levels o

233 BioID proximity labeling revealed that BCL9 and B9L, like PYGO2, are con

234 erometry (XSI) is based on site-specifically labeling RNA with pairs of heavy atom probes, and precis

235 we combined specific and segmental isotopic labeling schemes with dynamic nuclear polarization (DNP)

236 Transgenic cell labeling showed Tac1-Pet1 soma resident largely in the c

237 Stable isotope labeling (SIL) techniques have the potential to enhance

238 ermore, a transgenic mouse line specifically labeling SL cells showed that they send profuse axonal p

239 ling; this resulted in >100-fold increase in labeling speed in both mice and Drosophila, at the expen

240 cleotidyl transferase-mediated dUTP nick-end labeling staining.

241 n readily incorporate complementary chemical labeling strategies and we anticipate that it will be ap

242 insights into these processes, but new spin-labeling strategies are needed.

243 k therefore extends the use of bioorthogonal labeling strategies to problems of clinical relevance.

244 Here, we used a double-labeling strategy (varying both the distance between and

245 Here we designed a labeling strategy based on the bifunctional mCherry-2xCL

246 In summary, our new labeling strategy offers several advantages compared wit

247 , where the observed dynamics depends on the labeling strategy.

248 Quench-labeling studies in the presence of ZnEt2 reveal that Zn

249 Lastly, deuterium-labeling studies indicate that deuterium scrambling does

250 Immunohistochemical double labeling studies with YFP and serotonin antisera combine

251 faster substrate was excluded through (18)O-labeling studies.

252 ces mobaraensis, a successful bio-orthogonal labeling system was demonstrated.

253 By using a retrograde labeling system, we defined the requirement of CSNs in t

254 retations for both image-labeling and nuclei-labeling tasks (83% and 87%), as compared to the patholo

255 Here, we used a stable isotope labeling technique ((18)O and (2)H) to determine the ori

256 Our approach employs a novel hapten-specific labeling technique of hybridoma cells.

257 lyzed by ESI/qTOF/MS using MS/MS and isotope labeling techniques.

258 Current labeling technologies cannot distinguish between excitot

259 Labeling the entire cell membrane allows one to demonstr

260 t in ganglion and amacrine cells on P1, also labeling the horizontal cells by P10.

261 After labeling the hybridized biotin-miRNA with streptavidin-H

262 azoline-based imaging tools by fluorescently labeling the scaffold at this position.

263 ative analysis of glycans can be achieved by labeling their reducing end using fluorescent tags.

264 al tags CLIP, SNAP, Halo, and TMP for tissue labeling; this resulted in >100-fold increase in labelin

265 IME (PRobe Incorporation Mediated by Enzyme) labeling to couple surface DAT directly to fluorophore,

266 ed electron microscopy combined with genetic labeling to define the three-dimensional arrangement of

267 We used glycan TMT-labeling to improve electrophoretic migration and enable

268 photon Ca(2+) imaging and activity-dependent labeling to recruit the relevant neurons, was found capa

269 ies and demonstrate the utility of proximity labeling to study the regulation of LD proteomes.

270 rom MyD88(-/-) mice displayed similar GalNAz labeling to wild-type corneas, but labeling was reduced

271 teomic technique, isotope-coded affinity tag labeling, to quantify the extent of NO2(-)-dependent S-n

272 l deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) and phospho-histone H3 (PH3) staining t

273 l deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) staining, circulating levels of alanine

274 By combining trans-synaptic labeling, ultrastructural analysis, calcium imaging, opt

275 Here we apply photoaffinity labeling using a propofol derivative, meta-azipropofol,

276 tivity, we developed cysteine-stable isotope labeling using amino acids in cell culture (SILAC), a me

277 lowed for site-specific and ratio-controlled labeling using low label excess.

278 sma lumican FSR were measured based on (2) H labeling using tandem mass spectrometry.

279 ormed using velocity-selective arterial spin labeling (VSASL) and 3D image acquisition with whole-pla

280 Labeling was accomplished by maleimide coupling of NOTA

281 lel, and a kit procedure for routine (99m)Tc labeling was developed.

282 serotonin and YFP, whereas single serotonin labeling was observed in 36% and exclusive YFP labeling

283 Tyramine-like labeling was observed in AmTAR1-IR positive structures,

284 ar GalNAz labeling to wild-type corneas, but labeling was reduced and patchy on IL-1 receptor (IL-1R)

285 Here, using human in vivo deuterium labeling, we demonstrate that classical monocytes emerge

286 xpression and in vivo and in vitro metabolic labeling, we found that TbPSS2 (i) is necessary for norm

287 Using double-immunofluorescence labeling, we observed the potential colocalization of Tn

288 scopy in combination with site-directed spin labeling, we show that familial PD-associated variants a

289 and perfusion images by using arterial spin labeling were obtained for comparison.

290 this method inherently requires fluorescent labeling which has several drawbacks.

291 Labeling with a fluorescent beta2AR ligand shows the rec

292 By combining padlock probe RNA labeling with a single-cell analysis, a new approach eff

293 y purification), coupled with stable isotope labeling with amino acids in cell culture (SILAC)-based

294 In vivo tumor labeling with fluorescent agents may assist endoscopic a

295 sential for activation, followed by specific labeling with fluorescent moieties, we generated a novel

296 Stable isotope labeling with multiple reaction monitoring-mass spectrom

297 Double labeling with other molecular markers confirmed that the

298 lted in immediate enhancement of parenchymal labeling with the fluorescent dye Hoechst 33342.

299 erstanding of complex processes, fluorescent labeling with visible light fluorescent proteins such as

300 tly labeled GCGR and GLP-1R ECL mutants with labeling yield as high as 68%.