ライフサイエンスコーパス: laborious

1 DNA extraction using conventional methods is laborious.

2 ens has historically been time consuming and laborious.

3 rocesses, but studying them is difficult and laborious.

4 diagnosis in patients with PIDs complex and laborious.

5 res are available, mostly time-consuming and laborious.

6 l regulators of gene expression has remained laborious.

7 toring of molecule release are expensive and laborious.

8 which meiotic mapping is significantly more laborious.

9 f data provided makes analysis difficult and laborious.

10 zyme-linked immunosorbent assay (ELISA), are laborious.

11 perimental methods used for this purpose are laborious.

12 powerful approach, but it is currently very laborious.

13 ll populations, but their production remains laborious.

14 responding genes has typically been slow and laborious.

15 ical context is still relatively complex and laborious.

16 ry screens that are relatively expensive and laborious.

17 y gene mutations in cancer are expensive and laborious.

18 ion properly can often be time-consuming and laborious.

19 direct sequencing, which is both costly and laborious.

20 dies because the genotyping procedure can be laborious.

21 studying centromere genomics in biology are laborious.

22 biology, strain engineering remains slow and laborious.

23 methods is becoming increasingly complex and laborious.

24 but functional testing of identified VHHs is laborious.

25 ulture methods, which are time-consuming and laborious.

26 lysts usually involves multiple steps and is laborious.

27 The state-of-the-art methods required laborious adjustments of parameter values to achieve the

28 e without the need for sample preparation or laborious analytical methods.

29 gies is becoming more effective, it is still laborious and carries substantial risk of adverse events

30 dies; however, the labeling processes can be laborious and challenging for use in non-transfectable c

31 However, most genotyping methods are laborious and complex, and involve the use of multiple s

32 Current genotyping methods can be laborious and costly and rely on subjective data interpr

33 lse positives is reported, which can lead to laborious and costly attempts to provide biological vali

34 tterns of TF binding, but typically requires laborious and costly experiments for each TF-cell-type (

35 x matched to prediction samples which can be laborious and costly.

36 directed hPSC differentiation protocols are laborious and costly.

37 y putative reagents is often time-intensive, laborious and costly.

38 Genotyping mice by DNA based methods is both laborious and costly.

39 tion and high temporal resolution, but it is laborious and costly.

40 Ss) in vivo, though the process is extremely laborious and costly.

41 old-standard isotope dilution techniques are laborious and costly; therefore, anthropometric predicti

42 greatly reducing time spent on an otherwise laborious and demanding task.

43 etween a plant and its aerial environment is laborious and destructive with current techniques, makin

44 good agreement with those determined by more laborious and expensive conventional methods.

45 tal or ex vivo imaging approaches, which are laborious and expensive in vertebrate models.

46 olecules, but the standard catalysts require laborious and expensive preparations.

47 However, it is a laborious and expensive process, and by its nature, intr

48 tifs by nucleotide sequencing, however, is a laborious and expensive process.

49 ll molecule on the expansion of HSC/HPC is a laborious and expensive process.

50 fraction of phospholipids, normally involves laborious and expensive protocols.

51 ts, offering an efficient alternative to the laborious and expensive traditional methods.

52 Conventional serotyping methods are laborious and expensive.

53 with many disulfide bridges has proven to be laborious and general methods are lacking.

54 ods to measure its activity are indirect and laborious and have low sensitivity.

55 ophila, this powerful technique has remained laborious and hence seldom implemented.

56 ion mapping of CGRs has traditionally been a laborious and highly skilled process.

57 Yet, producing these substrates remains laborious and inefficient, particularly when using the t

58 has largely relied on methods that were both laborious and inefficient.

59 r microsporidia in clinical laboratories are laborious and insensitive and tend to underestimate thei

60 pulsed-field gel electrophoresis (PFGE), is laborious and insufficient for discriminating between cl

61 tical transparency have been key advantages, laborious and invasive procedures are required for elect

62 he identification of these regulators can be laborious and is usually carried out one factor at a tim

63 phospholipase C treatment, thereby avoiding laborious and long-lasting sample purification.

64 tegies for characterizing rearrangements are laborious and low-throughput and have low sensitivity or

65 However, the laborious and low-throughput methods of current yeast re

66 Yet it remains a laborious and low-throughput technique, making it largel

67 verall process of aptamer generation remains laborious and low-throughput.

68 able for adaptation; however, such review is laborious and may not always uncover useful guidelines.

69 s conventional methods for its diagnosis are laborious and not easily reproducible.

70 However, OFCs are laborious and not without risk; thus, a test that could

71 roteins in parallel on the chip surface, the laborious and often costly processes of DNA cloning, exp

72 But these standard methods can be laborious and only allow single amino acid substitution,

73 racellular electrode measurements, which are laborious and preclude analysis at the level of an entir

74 on from experimental micrographs thus can be laborious and presents a major practical bottleneck in c

75 or removing these fluctuations, which can be laborious and prone to subjective bias.

76 However, these techniques are laborious and require the use of relatively large amount

77 red phenotypes, these methods are limited to laborious and serial manipulation of single genes and ar

78 lated tuber-bearing potato relatives, but is laborious and slow.

79 Using current methods, however, it is laborious and sometimes difficult to generate antibodies

80 insects in the laboratory but this can be a laborious and sometimes fruitless process.

81 stoStitcher is still manual and hence can be laborious and subjective, especially when doing large co

82 ll culture systems; however, these tests are laborious and suffer decreased sensitivity for advanced-

83 hod is highly discriminatory; however, it is laborious and technically demanding, and data exchange r

84 rformance liquid chromatography not only was laborious and tedious but also could introduce uneven lo

85 o measure cooperativity parameters have been laborious and therefore limited to studying only a few s

86 still bioreactor-based, extremely expensive, laborious and time consuming and, furthermore, not autom

87 This is a laborious and time consuming process.

88 It can substitute the laborious and time-consuming analytical evaluation.

89 yme-linked immunosorbent assays (ELISA), are laborious and time-consuming and lack sensitivity.

90 tools for this purpose, these protocols are laborious and time-consuming and typically require the a

91 eria in food products as alternatives to the laborious and time-consuming culture procedures.

92 ource of internal standards and the need for laborious and time-consuming DNA digestion protocols con

93 gnificant because, in contrast to the common laborious and time-consuming heterogeneous binding appro

94 l performed in multiwell plates and requires laborious and time-consuming manual manipulation of spec

95 the field still needs to rely on a 50-y-old laborious and time-consuming method to assess the lifesp

96 operational costs, low throughput screening, laborious and time-consuming procedures, and ethical con

97 reading, and interpretation of Lyme WBs are laborious and time-consuming procedures.

98 However, this is a laborious and time-consuming process, and mass spectrome

99 ernative to established methods that require laborious and time-consuming sample treatments and expen

100 Raman spectroscopy) is only possible through laborious and time-consuming statistical analysis, which

101 However, these methods are associated with laborious and time-consuming steps, such as the generati

102 ors, because these adducts currently require laborious and time-consuming syntheses and purification.

103 binding sites is a technically challenging, laborious and time-consuming task, while computational m

104 this type of analysis is powerful, it can be laborious and time-consuming to characterize the large n

105 Conventional UDS and RRS assays are laborious and time-consuming, as they measure the incorp

106 repetitive wash steps with a pipet that are laborious and time-consuming, making the procedure ineff

107 electrophoresis-based detection procedure is laborious and time-consuming, often requiring visual ins

108 This approach was successful but highly laborious and time-consuming, precluding general applica

109 ess, the establishment of mutant ES cells is laborious and time-consuming, restricting the study of t

110 sition frequently use toxic solvents and are laborious and time-consuming.

111 ion and/or other molecular tests are usually laborious and time-consuming.

112 with restriction enzyme digestions, which is laborious and time-consuming.

113 f SEs, serological typing and PCR typing are laborious and time-consuming.

114 g T cell-recognized tumor antigens (Ags) are laborious and time-consuming.

115 Many methods employed are laborious and time-consuming; recent advances, translate

116 ucts, and conventional PCR-based methods are laborious and time-intensive.

117 rely on conventional animal models that are laborious and use limited numbers of animals; alternativ

118 ure-derived viral antigen for these tests is laborious and variable from batch to batch, and the resu

119 yet current detection techniques tend to be laborious and/or expensive.

120 n-transposon mediated gene targeting remains laborious, and as a result has not been widely used.

121 tively deuterated PUFAs require rather long, laborious, and expensive syntheses.

122 Serology is cross-reactive, laborious, and frequently difficult to interpret, and se

123 However, these genetic methods are laborious, and limited for quantitative study.

124 This work is laborious, and replication of the technique will be impo

125 usion (SRID) assay, which is time-consuming, laborious, and requires production of large quantities o

126 f animals with phosphopeptides is expensive, laborious, and time-consuming.

127 he process of analysis being time-consuming, laborious, and ultimately allowing only serial analysis

128 ore may aid the replacement of expensive and laborious animal models.

129 gation, effectively eliminating the need for laborious antigen purification from cell-culture express

130 n is transforming genetic engineering from a laborious art to an information-driven discipline.

131 These studies often involve laborious assays of multiple individual variants that ar

132 egulators is challenging and often relies on laborious assays or indirect reporter read-outs.

133 e NMR experiments and easier to compile than laborious assignments of non-solvent-exchangeable proton

134 A-origami structure through AGE is typically laborious because of the post-electrophoresis extraction

135 red for new or altered function, however, is laborious because the engineering must be replicated for

136 overy of common light chains, or complex and laborious biochemical processing.

137 apped bead, without the need of embarking on laborious calculations.

138 rgeting large-insert clones using costly and laborious capillary-based sequencing approaches.

139 abels, which involves rather complicated and laborious chemical synthesis.

140 Hence, it is necessary to carry out laborious chromatographic purification steps or to make

141 L2) learning is often claimed to be slow and laborious compared to native language (L1) acquisition,

142 e previously required, but the procedure was laborious, complex, and technically challenging.

143 essential proteins are usually expensive and laborious, computational methods, which utilize biologic

144 egulatory regions traditionally involves the laborious construction of promoter deletion series which

145 operties unavailable by other means, but the laborious deposition process and multiple dipping cycles

146 Using this feature, the conventional and laborious dry-down step prior to reaction is circumvente

147 ntly, mRNA-based reprogramming is relatively laborious due to the need to transfect daily for ~2 week

148 ent (IRE) without the need for polishing and laborious edge preparation.

149 n to be in excellent agreement with the more laborious electrophoretic approaches that are widely use

150 nsert transfer process is time consuming and laborious especially if many inserts are to be subcloned

151 , which allows estimating the feasibility of laborious experimental assays and aids in their design.

152 uming, trial-and-error process that requires laborious experimentation.

153 limited by a dependence on haplotyping from laborious experiments or extra genome/family trio data.

154 t determining disease-related genes requires laborious experiments.

155 of the sensor without need of extensive and laborious extraction processes are demonstrated in water

156 igned by a process that involves tedious and laborious filtering of potential candidates against a se

157 special case of image segmentation, which is laborious for humans and would ideally be performed by c

158 IGS but this method has limitations as it is laborious for large-scale screening and some plants are

159 a trans-blocking strategy, which requires a laborious gel separation and extraction step.

160 t be targeted to a specific gene and require laborious gene mapping and sequencing.

161 lian system in vivo, we sought to bypass the laborious generation of a transgenic animal by exploitin

162 While previous studies have relied on laborious genetic modification of cells and extensive gr

163 y metabolic disease models still depend upon laborious germline targeting, and are further complicate

164 Often the process is laborious, goes unrecorded and may be biased.

165 and engineer stable, active enzymes without laborious high-throughput activity screens.

166 However, these are largely dependent on laborious histological analysis and immunohistochemical

167 d robust system has the potential to replace laborious identification schemes in field environments.

168 ic craniofacial procedures mainly due to the laborious implantation requirements.

169 nial (SSG) susceptibility of Leishmania is a laborious in vitro assay of which the result has little

170 ounding each genome edit, which necessitates laborious in vitro DNA splicing.

171 novo genome assemblies without the need for laborious in vivo cloning steps.

172 ods for detecting DNA rearrangements require laborious indirect assays.

173 aged proteins is nontrivial, with subsequent laborious introduction into living cells, potentially pr

174 icability in standard quality control due to laborious isolation and purification steps.

175 ntly available research tools, which involve laborious isolation, separation and characterization ste

176 tivity with metal ion binding, necessitating laborious iterative substrate optimization.

177 As such, it offers important advantages over laborious layer-by-layer methods for solar cells and pho

178 onal assays will eventually replace the more laborious, less physiologic, and less informative approa

179 ut constructing conditional alleles is often laborious, limited by target gene structure, and at time

180 A protocol-by-protocol approach, though laborious, makes sense for both investigators and IRBs a

181 and it has been carried out in a similar and laborious manner over many decades.

182 Compared to laborious manual analysis of the MS/MS spectra, the pres

183 which would otherwise require extensive and laborious manual assessment.

184 tinuously separated from their progeny via a laborious manual microdissection procedure.

185 MS (LC-MS/MS) runs remains a challenging and laborious manual process.

186 To eliminate laborious manual processing of samples, and to minimize

187 hallenges that are often only addressable by laborious manual validation, including data set error, d

188 equent verification of iPSC pluripotency are laborious, manual processes limiting the scale and repro

189 applicable to many systems, does not rely on laborious marker development or genotyping, does not req

190 have evolved during the past few years from laborious measurements on discrete samples to continuous

191 vity, obviating the need for purification or laborious membrane reconstitution procedures.

192 tabolic products is hindered by the slow and laborious methods available for evaluating product conce

193 tion of binding sites requires expensive and laborious methods such as mutagenesis and binding essays

194 ization of polymer brush formation relies on laborious methods that use a quartz crystal microbalance

195 They require complex and laborious methods to locate, and many still lack methods

196 by sophisticated and expensive equipment and laborious methods.

197 is of similar sensitivity to existing, more laborious methods.

198 an replace, in routine analysis, complex and laborious MS methods in the study of lignin composition.

199 s has been impeded by equipment cost and the laborious nature of making measurements one molecule at

200 ple size, as they were when mapping required laborious on-ground survey.

201 lent labeling of the enzyme to the antibody, laborious operating procedures, and extensive washing st

202 metals require expensive instrumentation and laborious operation, which can only be accomplished in c

203 roaches require bisulfite treatments and are laborious or costly to perform.

204 xistence of reference datasets, which may be laborious or expensive to collect.

205 f the four polymorphisms are either slow and laborious or extremely expensive.

206 can HILIC analyses have however tended to be laborious or require compromises in sensitivity.

207 lonies by PCR, due to being too lengthy, too laborious or yielding poor quality DNA and inconsistent

208 o replace the reliance on more expensive and laborious PCR assays.

209 It will make laborious PCR based technique less obligatory as it allo

210 ongation complexes using EcoRIE111Q requires laborious PCR using many different oligonucleotides for

211 recruit altered reading circuits and rely on laborious phonology-based "sounding out" strategies into

212 samples, (ii) circumventing the traditional laborious picking and functional testing of individual p

213 circuits control which behaviors has been a laborious piecemeal process, usually for one observer-de

214 Many recent techniques still involve laborious pipetting steps for spheroid manipulation such

215 (RNA-seq), we tested whether we could avoid laborious positional cloning for the isolation of TALE-i

216 of phosmet in olive oil samples without any laborious pre-treatment.

217 is advantageous since it avoids the need for laborious prederivatization and cleanup steps.

218 A lengthy and laborious procedure is an additional challenge of protei

219 itu humanization), but such efforts involved laborious procedures and were limited in size and precis

220 ic ion channels is contrasted with existing, laborious procedures that require membrane protein extra

221 Gap filling is a laborious process because it relies on analysis of rando

222 specifically recognize protein targets is a laborious process if conducted in a one protein-one comp

223 s of AT are limited, however, because of the laborious process of collecting and analyzing samples.

224 y automated HPLC-MS methods, followed by the laborious process of identifying the structure of the me

225 rmation is limited in databases owing to the laborious process of literature-based curation.

226 nowledge or upon a somewhat unsystematic and laborious process of manually searching through the lite

227 NA fragments, and are tedious because of the laborious process of multiple plaque purifications, whic

228 way ligation followed by gel purification, a laborious process that often leads to low yield both in

229 affairs and to cut labor costs and expedite laborious processes in mining, tunneling, construction,

230 nerships, bring about revision of costly and laborious processes of licensing and regulation of new a

231 strategy we employ bypasses the traditional laborious processes to elicit gene cluster expression an

232 sue culture, involving complicated, long and laborious processes.

233 because of difficult material synthesis and laborious processing.

234 Ziehl-Neelsen is laborious, prone to human error and infection risk, with

235 it mapping using electron microscopy demands laborious proofreading or reconciliation of multiple ind

236 ving as compared to conventional LC-MS where laborious protein precipitation or other sample pretreat

237 zyme- introduced labeling and sequence bias, laborious protocols, high experiment-to-experiment varia

238 rs is usually based on libraries produced by laborious protocols.

239 ies require specialised bacterial strains or laborious protocols.

240 roved techniques for humanizing mice without laborious purification of HSC.

241 ly of donor blood or bone marrow samples and laborious purification of mature myeloid cells or progen

242 low-yielding, requiring long reaction times, laborious purification steps and often extreme condition

243 The method generally involves laborious radioactive gel electrophoresis and is not con

244 However, this approach requires laborious reagent generation procedures and entails unpr

245 their use in medical research is hampered by laborious reprogramming procedures that yield low number

246 These additional techniques are either laborious, require specialized equipment, or are not qua

247 Rather than the laborious review of individual slides and issues of assa

248 Traditional methods utilize harsh and laborious sample dehydration procedures, often leading t

249 es) often require multiple dilution steps or laborious sample preparation prior to spectroscopic anal

250 pathogenic microorganism detection without a laborious sample preparation process and without the use

251 to many types of protein drugs but with the laborious sample preparation steps (SDS-PAGE and the sub

252 commercial formulations without the need for laborious sample preparation steps.

253 ailable but can suffer from low selectivity, laborious sample preparation, and/or the need for comple

254 paration, requiring metabolism quenching and laborious sample preparation, the objective of the study

255 ain rely on extensive purification requiring laborious sample preparation.

256 ure facility emissions, without conventional laborious sample pretreatment.

257 or laboratory science, but a drawback is the laborious sample processing required before MS analysis.

258 wever, the assay's low sample throughput and laborious sample workup procedure are limiting factors t

259 scribed because their discovery required the laborious screening of patient tumour-infiltrating lymph

260 The scope of DNA microarrays allows for less laborious screening on a much larger scale than possible

261 This method, however, requires the laborious selection of aptamers with high target specifi

262 ts in quantitative traits that often rely on laborious selection of rare naturally occurring mutation

263 egy for the four porphyrin species without a laborious separation process.

264 however, these analyses are time-consuming, laborious, subjective and lack sensitivity and specifici

265 informative of underlying pathology as more laborious subtype diagnoses.

266 ic coupling via reactive esters requiring no laborious surface pretreatment.

267 parable results, use of the cheaper and less laborious swab technique is acceptable and may be prefer

268 interactions, but a limitation has been the laborious synthesis of glycan structures by enzymatic an

269 onventional genotoxicity testing methods are laborious, take time and are expensive.

270 arallelizable approach that does not require laborious target-specific purification to generate dual-

271 but identifying the causative gene can be a laborious task.

272 cells in culture and alleviate the need for laborious testicular transfers in initial cell culture s

273 ficulty validating their predictions without laborious testing in 'wet' labs.

274 etabolic engineering in plants relied on the laborious testing of ad hoc modifications to achieve des

275 ment course is substantially more costly and laborious than for drug-susceptible tuberculosis, with h

276 t was then analysed by RPLC-GC, avoiding the laborious thin layer chromatography step used in the Off

277 atic mutations in cancer tissue is extremely laborious, time-consuming and costly.

278 tractable, plant trait engineering remains a laborious, time-consuming and unpredictable undertaking.

279 ification for proteomic study, side stepping laborious, time-consuming sample preparation steps such

280 apeutic drugs in biofluids typically require laborious, time-consuming, and expensive analytical work

281 lity and automation compared with common but laborious titration approaches, and greatly improves scr

282 low, subject to user bias, and sometimes too laborious to achieve sample sizes large enough to detect

283 typing techniques are too time-consuming and laborious to be carried out within the limited time cons

284 ssays either have technical drawbacks or are laborious to carry out.

285 is a valuable protein characteristic but is laborious to improve experimentally.

286 ore potent therapeutically and they are more laborious to prepare, future studies should focus on uns

287 onal Ab formats (IgG, IgM) are expensive and laborious to produce; however, the introduction of new r

288 l and pathological processes; however, it is laborious to study sialylated glycoproteins due to the l

289 tablishing epigenetic landscape without more laborious transcriptional regulations.

290 lar interactions without the requirement for laborious transfection, cell culture, or protein purific

291 nd commonly employed, but generally requires laborious trial-and-error approaches.

292 lly materials discovery has been driven by a laborious trial-and-error process, knowledge-driven mate

293 milar to those for the conventional but more laborious tube-based extraction.

294 Could (or should) this be the laborious, uncertain, and high-risk trajectory of disrup

295 nt of the property of interest, oftentimes a laborious undertaking.

296 enomic loci in living cells has proven to be laborious until the recent development of CRISPR-Cas9-ba

297 vs intercellular CO2 concentration, Ci ) is laborious, which limits availability of Vcmax data.

298 ructures are technologically challenging and laborious, which makes the development of computational

299 er's curse biases, which are reduced only by laborious winner's curse adjustments (WCAs).

300 g technologies have been shown to reduce the laborious work involved in organizing the vast amount of