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1 the Escherichia coli lactose uptake network (lac operon).
2 observed in autocatalytic systems (e.g. the lac operon).
3 highest affinity for the lac PI site in the lac operon.
4 hanism might contribute to regulation of the lac operon.
5 els of Th-lymphocyte differentiation and the lac operon.
6 ase expression that trigger induction of the lac operon.
7 ng in the regulation of the Escherichia coli lac operon.
8 genetic systems, including the prototypical lac operon.
9 positive feedback architecture, such as the lac operon.
10 protein that regulates transcription of the lac operon.
11 m, whether polymorphic or monomorphic at the lac operon.
12 expression that was first documented in the lac operon.
13 d for adaptive point mutation in the E. coli lac operon.
14 se analogues as measured by induction of the lac operon.
15 ct in a fully additive manner in vivo at the lac operon.
16 We show that this approach applied to the lac operon accurately predicts the system behavior for a
17 is under the transcriptional control of the lac operon and is inducible with isopropyl-1-thio-beta-D
19 nsights into the detailed functioning of the lac operon and reveal an efficient avenue to incorporate
20 ors that allow regulated expression from the lac operon and T7 promoters whose biology is well unders
21 er the past half-century, the details of the lac operon and the allosteric model have been tested usi
23 One sequence was derived from the bacterial lac operon and the other was created by re-encoding the
24 oli B/r were used; one has wild-type spc and lac operons and the other has a lac operon deletion, a w
25 lex environments, population dynamics of the lac operon, and the synchronization of genetic oscillato
27 ting environments using the Escherichia coli lac operon as a model system for genotype-environment in
29 ffect was investigated by placing the mutant lac operon at many sites in the genome of Salmonella ent
30 , which is analogous to the induction of the lac operon at subsaturating inducer concentrations in la
32 ated CCR when lactose was used to induce the lac operon, but resulted in reduced lac gene expression
33 ion of transcription of the Escherichia coli Lac operon by the Lac repressor (LacR) is accompanied by
35 recombinant expression systems that utilize lac operon control elements to modulate gene expression
36 hage T7 RNA polymerase gene is expressed via lac operon control elements, exhibits leaky expression t
37 We have constructed a series of monocopy lac operon control region constructs in which the primar
39 type spc and lac operons and the other has a lac operon deletion, a wild-type spc operon, and a Pspc-
41 scriptional and translational fusions to the lac operon demonstrated that while FljA inhibits fliC tr
46 larities to and differences from that of the lac operon first described 50 years ago by Novick and We
49 cay of the rplN section of mRNA from the spc-lac operon fusion was coupled to the decay of the downst
52 cripts by RNase T2 protection assays and for lac operon fusions to either the flgAMN or flgMN promote
53 e response to heme starvation of a number of lac operon fusions to the hemA promoters of both E. coli
54 twofold) increase in the expression of hemA-lac operon fusions; thus HemA regulation is mediated eit
55 coli protein abundance upon induction of the lac operon genes using isopropyl beta-D-thiogalactopyran
56 p a reaction network for the dynamics of the lac operon genetic switch and derive corresponding deter
61 been used to describe the regulation of the lac operon in E. coli and the lysis/lysogeny switch of p
63 ) efficiently represses transcription of the lac operon in Escherichia coli by binding to two distant
66 ystems, and suggest that derepression of the lac operon in the absence of inducer may be part of a ge
67 nd operator (DNA) and its interplay with the lac operon inducer isopropyl beta-D-1-thiogalactopyranos
73 e to produce allolactose (the inducer of the lac operon) is slower with the mutant than with the nati
74 why allolactose, the natural inducer for the lac operon, is the preferred product of transglycosylati
75 y than the naturally occurring switch of the lac operon; it is less leaky and can be induced more eff
76 on of bacterial cells with a mutation in the lac operon (lac-) accumulates Lac+ revertants during pro
83 the fhuA polymorphism, and consequently the lac operon polymorphism, was lost between 86 and 219 gen
88 dence of looping-dependent repression of the lac operon provides insight into DNA deformation energet
89 of 13 chemostat cultures monomorphic for the lac operon retained the neutral fhuA polymorphism for 45
91 IBCG, an inducer of genes controlled by the lac operon system in bacterial cells, was achieved in 5
92 cellular processes, an inducible p21 vector (lac operon system) was transfected into the rat pheochro
93 mework is applied to the extensively studied lac operon system, the SOS response system and the araBA
95 These findings explain control points in the lac operon that minimize the cost of lac permease activi
97 ping with components of the Escherichia coli lac operon to monitor DNA flexibility in living cells.
99 l inducer/repressor motifs homologous to the lac operon to the recently discovered interfering RNAs.
100 hat would restore function to an inactivated lac operon unexpectedly yielded fusions in which lac was
101 technology derived from the Escherichia coli Lac operon, uninduced transgenes are kept in a repressed
102 scriptional and translational fusions to the lac operon using FLP mediated site-specific recombinatio
104 During exponential growth, lacZ mRNA of the lac operon was translated about twice as frequently as l
105 by chance, I generated strains in which the lac operon was transposed from its normal position on th
106 Within a simple mathematical model for the lac operon, we show that regulation based on DNA looping
107 niversary of the landmark publication of the lac operon with a review that provides a current view of
108 been known that gratuitous induction of the lac operon with non-metabolizable lactose analogues gene
109 oring stochastic epigenetic switching in the lac operon (with and without an error-prone transcriptio
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