ライフサイエンスコーパス: laccase

1 ic mechanism of estrogens' transformation by laccase.

2 yl compounds) in the absence and presence of laccase.

3 Rutin and esculin have been polymerised by laccase.

4 produced by the cell wall-associated enzyme laccase.

5 m a mutant defective in the virulence factor laccase.

6 an intermediary transcriptional repressor of laccase.

7 al structural data on rusticyanin and fungal laccase.

8 rmediates analogous to that observed in T1Hg laccase.

9 ltiple-copper oxidases ascorbate oxidase and laccase.

10 und in domain II of ceruloplasmin and fungal laccase.

11 y cryptococcal cells, which is mediated by a laccase.

12 aw compared with the same system lacking the laccase.

13 asensitive detection of polyphenols by using laccase.

14 manifested by flavonoids in the presence of laccase.

15 er reaction, can be efficiently catalyzed by laccases.

16 ion and engineered expression of prokaryotic laccases.

17 which predominantly produce lower-potential laccases.

18 rial quantities of designer, fit-for-purpose laccases.

19 mulation of transcripts for plantacyanin and laccases.

20 Insects injected with dsRNA for the laccase 2 gene failed to tan, were soft-bodied and defor

21 support the hypothesis that two isoforms of laccase 2 generated by alternative splicing catalyze lar

22 phenoloxidases tested, with the exception of laccase 2.

23 In the absence of laccase, a significant portion of the added SMZ formed c

24 eoformans virulence through the induction of laccase, a Th2-skewing and CNS tropic factor.

25 lts showed that EE2 was degraded by isolated laccase (about 90% within 24 h).

26 s study provides fundamental information for laccase-ABTS mediated labetalol reactions and the effect

27 The Laccase/ABTS/C composite was characterised by Fourier Tr

28 fuel cell composed of an enzymatic cathode (Laccase/ABTS/C) and an inorganic anode (AuAg/C) was deve

29 properties showed highly conserved nature of laccases across three cotton species.

30 bited decreased extracellular peroxidase and laccase activities and showed defects in colony pigmenta

31 (r = -0.28, P = 0.05) and exhibited enhanced laccase activity (r = 0.36, P = 0.003).

32 lemented with wild-type CLC-A which restored laccase activity and melanin biosynthesis.

33 in vivo and the effect of this regulation on laccase activity and melanin synthesis.

34 is thaliana resulting in increased secretory laccase activity and the enhanced resistance to trichlor

35 Based on the inhibition of laccase activity and using 4-aminophenol as redox mediat

36 Deletion of the C1 domain repressed laccase activity and, consequently, melanin production.

37 Surprisingly, laccase activity assays revealed that Deltassa1 was not

38 Fungal isolates with greater laccase activity exhibited heightened survival ex vivo i

39 de channel gene CLC-A which is essential for laccase activity in C. neoformans.

40 This accurate deposition ensures that laccase activity is highest where new leaf material ente

41 accase triple mutant, suggesting that lignin laccase activity is necessary and nonredundant with pero

42 w laccase is involved, we tested recombinant laccase activity on the prostaglandin precursors, arachi

43 2)=0.9367, adjusted R(2)=0.8226) under which laccase activity reached 2000 +/- 100 Ug(-1) of beads, w

44 Laccase activity was substantially restored in metabolic

45 Laccase activity, which is mediated through small organi

46 ctron microscopy, despite a complete lack of laccase activity.

47 iferation rate (IPR), capsule induction, and laccase activity.

48 re degradation reached 50% mainly due to the laccase activity; however, after a supplementation with

49 Laccase adsorption to DOM adlayers on amine-terminated S

50 e/air enzymatic fuel cells (EFCs), EFCs with laccase air-breathing cathodes prepared from TBA(+) modi

51 ble mutant functions kinetically better as a laccase, albeit a relatively inefficient one.

52 ensitizer with the multicopper oxidase (MCO) laccase allows to link the oxidation of an organic molec

53 Next we investigated cryptococcal laccase, an enzyme known to bind polyphenols, and found

54 f methanol:buffer of pH 5 using 2300 U/mg of laccase and 5mM of catechin.

55 The overall structure is similar to those of laccase and ascorbate oxidase, but contains an extra 42-

56 hermore, deletion of Ssa1 results in reduced laccase and attenuated virulence using a mouse model.

57 scopy demonstrate that the deposited enzymes laccase and catalase by means of AC-EPD did not inhibit

58 ncreased reduction potentials of C. cinereus laccase and human ceruloplasmin.

59 Catechin was oligomerized using free laccase and laccase-gum Arabic conjugate.

60 es have been implicated in the regulation of laccase and melanization, including IPC1, GPA1, MET3, an

61 of the HPTS fluorescence in the presence of laccase and on the activating effect of Tb4O7NPs, which

62 whereas the reverse is true for C. cinereus laccase and T. ferrooxidans rusticyanin.

63 nol radical cation generated directly by the laccase and the CF3-radical.

64 ol activates both melanin production through laccase and transcription of antiphagocytic protein, bot

65 Two kinds of phenoloxidases, laccase and tyrosinase, have been proposed to participat

66 three virulence factors (capsule production, laccase and urease expression), as well as a growth defe

67 ulence factors including a copper-containing laccase and was avirulent in a mouse model.

68 will facilitate intramolecular ET in fungal laccases and Fet3p.

69 Experiments with laccases and other catalysts like a Co(salen) type catal

70 position) as in ceruloplasmin, Fet3p, fungal laccases and some plantacyanins (PLTs).

71 We report the cloning of cDNAs for laccases and tyrosinases from the red flour beetle, Trib

72 superoxide dismutase, lactate dehydrogenase, laccase) and damage (thiobarbituric acid reactive substa

73 catechins were incubated with tyrosinase and laccase, and product formation was monitored by RP-UHPLC

74 that they also target superoxide dismutases, laccases, and ATP sulfurylases.

75 Laccases are a class of multi-copper oxidases (MCOs) tha

76 Here, we provide evidence that laccases are also involved in the lignification of Brach

77 Laccases are blue multicopper oxidases that catalyse the

78 In dicots, both peroxidases and laccases are known to participate in this process.

79 These results provide clear evidence that laccases are required for B. distachyon lignification an

80 Although fungal laccases are well known and well characterized, only rec

81 roperties of OTS and simultaneously produced laccase as a beneficial co-product.

82 electrochemical data on Thermus thermophilus laccase as benchmarks to validate our model, which we su

83 ty of the copper-containing virulence factor laccase as well as almost normal growth at 37 degrees C

84 erulate (EF) with Myceliophthora thermophila laccase, as biocatalyst, was performed in aqueous medium

85 or the immobilization of glucose oxidase and laccase at the anode and cathode respectively; no extern

86 developing EFC using AOx based bioanode and laccase based biocathode without applying any toxic free

87 rk was to use of electroanalytical tools and laccase based biosensor on the evaluation of AOC and tot

88 otal phenol content was estimated by using a laccase based biosensor.

89 tential, useful for an ever-growing range of laccase-based applications.

90 t between the conductive support and soluble laccase biocatalyzing oxygen reduction.

91 rate in a fuel cell setup using air breathed laccase biocathode.

92 eption, the bioanode was combined with AuNPs-laccase biocathode.

93 Finally, laccase bioelectrodes were employed within an enzymatic

94 Oxygen-reducing laccase bioelectrodes were found to be inhibited by both

95 in this work was finalized to the setup of a laccase biosensor based on a multilayer material consist

96 The developed laccase biosensor has responded efficiently to caffeic a

97 sent work, we demonstrate the fabrication of laccase biosensor to detect the catechol (CC) using lacc

98 A Laccase biosensor was applied to the selective determina

99 enzymatic activity of oxidoreductases (i.e., laccase) both in vivo and in vitro, which is usually mea

100 By employing tyrosinase and laccase, both from Agaricus bisporus, on green tea catec

101 The reversible inhibition of laccase by arsenite (As(3+)) and arsenate (As(5+)) is re

102 of one of the copper atoms (type 2) of tree laccase by nitric oxide (NO) has been detected.

103 Here we present for the first time that laccase can catalyze electrooxidation of H2O to molecula

104 or understanding and targeting the Ipc1-Pkc1-laccase cascade as a regulator of virulence of this impo

105 This laccase-catalysed trifluoromethylation proceeds under mi

106 ent between 400 and 500 cm(-1) in spectra of laccase catalytic membranes, demonstrating the potential

107 henol oxidases (PPOs) such as tyrosinase and laccase catalyze the enzymatic oxidation of PCs and thus

108 Laccase catalyzed the formation of covalent bonds by oxi

109 The laccase-catalyzed domino reaction between catechols and

110 btained in yields ranging from 39% to 98% by laccase-catalyzed domino reactions between hydroquinones

111 itosan and its derivatives functionalized by laccase-catalyzed oxidation of ferulic acid (FA) and eth

112 labetalol can be effectively transformed by laccase-catalyzed reaction using 2, 2-Azino-bis-(3-ethyl

113 Moreover, the reaction pathways of laccase-catalyzed transformation of E2 were proposed.

114 s well as the decomposition of E2 into E1 by laccase-catalyzed treatment, has been demonstrated by li

115 In this reaction, laccase catalyzes diferulic acid (diFA) formation to for

116 thod enables the comparative quantitation of laccase characteristics (i.e., profiles of activity at v

117 in Rhus vernicifera and Trametes versicolor laccase, characterized by "normal" type 2 Cu electron pa

118 We identify nine putative laccase-coding genes in the fungal genome of Leucocoprin

119 m a preserved activity of the tyrosinase and laccase combined with the electron transfer activity of

120 ne, varied over 2 orders of magnitude by the laccase concentration in the picomolar range.

121 pper homeostasis and targets a member of the laccase copper protein family.

122 pper protein plantacyanin and members of the laccase copper protein family.

123 The higher enzymatic activity of laccases correlated with higher lignin content at 25 DPA

124 of two decades ranging from 0.5 to 75 ng of laccase (corresponding to enzymatic activities from 62 x

125 Laccase could act as a biocatalyst for oxygen reduction

126 rature, while, in contrast, activity of free laccase declined to 60% of its initial activity.

127 ivity assays revealed that Deltassa1 was not laccase deficient, demonstrating that H99 does not requi

128 of congenic laccase-positive (2E-TUC-4) and laccase-deficient (2E-TU-4) strains, we found that both

129 n of cryptococcal-phagocyte interactions and laccase-dependent melanin pathways to human clinical pre

130 A purified laccase (designated lcc3) was identified by LC-ESI MS/MS

131 he same class as nitrite reductase domain 2, laccase domain 3 and ceruloplasmin domains 2, 4 and 6.

132 and intestinal myeloid-derived cells express laccase domain-containing 1 (LACC1); LACC1 is expressed

133 may reduce effective cell wall targeting of laccase during infection of the lung but not during infe

134 The laccase employed in our study does display reactivity-re

135 nic yeast Cryptococcus neoformans produces a laccase enzyme (CNLAC1), which catalyzes the synthesis o

136 Laccase enzyme can convert polyphenols to yield mono/pol

137 and subsequent modification of a particular laccase enzyme for the detoxification of secondary plant

138 understood, but extracellular peroxidase and laccase enzymes appear to be involved.

139 The multifunctional laccase enzymes play important roles in cell elongation,

140 sented that utilize commercial cellulase and laccase enzymes, which are known to modify major polymer

141 measurements on single-turnover processes in laccase established fast type-1 Cu to trinuclear Cu clus

142 Our initial study of Rhus vernicifera Laccase experimentally established that the native inter

143 Cryptococcus neoformans laccase expression during murine infection was investiga

144 analysis revealed that the basis for absent laccase expression in the clc-a mutant was a laccase tra

145 two important virulence factors, capsule and laccase expression, which are required for virulence of

146 nstrating that H99 does not require Ssa1 for laccase expression, which explains the CNS tropism we st

147 poly (4-vinylpyridine) was used to wire the laccase for electron transfer in the biocathode.

148 rated for 100 h with minimal requirements of laccase for the transformation of estrone (E1), 17beta-e

149 The amount of laccase found in different sites varied as a function of

150 to the cell wall was suggested by removal of laccase from cell wall preparations after they were boil

151 ovalent immobilization of recombinant POXA1b laccase from Pleurotus ostreatus on epoxy activated poly

152 ates between the copper centers of the small laccase from Streptomyces coelicolor at room temperature

153 hloroperoxidase, horseradish peroxidase, and laccase from T. versicolor).

154 in-fused variant of a recently characterized laccase from the aerobic bacterium Thermobifida fusca Th

155 n of cell wall integrity and displacement of laccase from the cell wall.

156 of the screening method with the commercial laccase from the fungus Trametes versicolor.

157 The model enzymes selected included the laccase from Trametes versicolor, the laccase-like enzym

158 quinone glucose dehydrogenase (PQQ-GDH) and laccase functioning as the anodic and cathodic catalyst,

159 In addition, a green fluorescent protein-laccase fusion protein demonstrated aberrant localizatio

160 A laccase gene from cotton was overexpressed in Arabidopsi

161 lant species, suggesting that the monolignol laccase genes diverged after the evolution of seed plant

162 Phylogenetic analysis revealed that lignin laccase genes have no orthologs in lower plant species,

163 abundance of monolignol biosynthetic genes, laccase genes, and certain peroxidase genes, suggesting

164 dii) cotton species identified 84, 44 and 46 laccase genes, respectively.

165 The laccase-gum Arabic conjugate showed lower activity but h

166 chin was oligomerized using free laccase and laccase-gum Arabic conjugate.

167 High potential purified Trametes trogii laccase has been deposited in mono- and multilayer thin

168 etween single and multisubstrate kinetics of laccases has been demonstrated.

169 ors based on phenol oxidases (tyrosinase and laccase) has been developed.

170 Bacillus pumilus, previously identified as a laccase, has been studied and characterized as a new bac

171 In the present study, various laccases have been applied to develop protocols that all

172 Fungal laccases have high activity in degrading various persist

173 Structural analysis of the laccase identified a C-terminal region unique to C. neof

174 mposite also providing compatible matrix for laccase immobilisation.

175 l behavior of laccase was investigated using laccase immobilized different modified SPCEs, such as GR

176 Compared with laccase immobilized GR and CMF modified SPCEs, a well-de

177 of Cu(I)/Cu(II) for laccase was observed at laccase immobilized GR-CMF composite modified SPCE.

178 biosensor to detect the catechol (CC) using laccase immobilized on graphene-cellulose microfibers (G

179 Native and laboratory-evolved laccases immobilized onto electrodes serve as bioelectro

180 eted Deltaclc-a mutant produced undetectable laccase in a liquid assay and produced no melanin on asp

181 H+)-ATPase proton pump in copper assembly of laccase in C. neoformans.

182 These data demonstrate a critical role for laccase in cryptococcal prostaglandin production, and pr

183 ower activity but higher stability than free laccase in methanol.

184 ess the catalytic role of the oxidoreductase laccase in the binding of sulfamethazine (SMZ) to Leonar

185 Addition of NO to native laccase in the presence of oxygen leads to EPR changes c

186 Overall data indicate significant roles of laccases in cotton fiber development, and presents an ex

187 rding expression of high reduction potential laccases in heterologous hosts, and issues regarding enz

188 the blue copper oxidase, Trametes versicolor laccase, in which the rate of change of the SWCNT device

189 s virulence mechanism in H99 is distinct and laccase-independent.

190 ption factor and TATA-binding protein during laccase induction.

191 Laccase inhibitors (azide and fluoride anions), pH optim

192 Laccase is a major virulence factor of the pathogenic fu

193 Laccase is a major virulence factor required for infecti

194 Laccase is a multicopper oxidase that contains four Cu i

195 To determine how laccase is involved, we tested recombinant laccase activ

196 d as a new platform in the immobilization of laccase (LAC) originating from Aspergillus oryzae.

197 genetic deletion of the primary cryptococcal laccase (lac1 Delta) resulted in a loss of cryptococcal

198 thetic genes and a secondary wall-associated laccase (LAC4) gene.

199 Although two recently discovered laccases, LAC4 and LAC17, have been shown to play a role

200 n experiments demonstrated that at least two laccases (LACCASE5 and LACCASE6) are present in lignifyi

201 sent such strategy for obtaining a DET-based laccase (Lc) cathode for O(2) electroreduction at low ov

202 and the multicopper oxidases (MCOs), such as laccase (Lc), and play vital roles in O(2) respiration.

203 One of these laccases (LgLcc1) is highly expressed in the specialized

204 oreover, the intracellular fraction and also laccase-like activity associated with fungal mycelium we

205 idation and oxygen reduction to evaluate the laccase-like catalysis of the materials, among which gam

206 ured manganese oxides, e.g. MnO2, have shown laccase-like catalytic activities, and are thus promisin

207 s, and the results correlate well with their laccase-like catalytic activities.

208 ed the laccase from Trametes versicolor, the laccase-like enzyme isolated from Bacillus subtilis, Cue

209 echanisms of and the factors controlling the laccase-like reactivity of different manganese oxides na

210 We have systematically compared the laccase-like reactivity of manganese oxide nanomaterials

211 Laccase mainly formed insoluble complexes.

212 ed to non detectable values of 7.9% for free laccase, manganese peroxidase (MnP), lignin peroxidase (

213 roup was the microcapsules cross-linked with laccase (MCL), the second group was the microcapsules cr

214 e hydrogel polymeric network is formed via a laccase-mediated cross-linking reaction.

215 ydrogel networks via oxygen consumption in a laccase-mediated reaction.

216 nto Polyacrylamide/pectin, 94%, 98%, 88% for laccase, MnP and LiP encapsulated respectively into poly

217 se (LiP), respectively; to 94%, 97%, 93% for laccase, MnP and LiP entrapped into Polyacrylamide/pecti

218 crylamide/ gelatine and to 87%, 91%, 87% for laccase, MnP and LiP entrapped, respectively into polyac

219 ally eat, and we confirm that these ingested laccase molecules pass through the ant guts and remain a

220 iltrating leukocytes than did infection with laccase-negative cells.

221 For laccase of T. versicolor (E(e)(0) = 0.82), the optimum m

222 reducing enzyme electrodes are prepared from laccase of Trametes versicolor and a series of osmium-ba

223 The cathode consisted of immobilized laccase on functionalized graphite electrode with 4-(2-a

224 direct immobilization of Trametes versicolor laccase on graphene doped carbon paste electrode functio

225 Immobilized laccase on the surface of a modified graphite electrode

226 w type of biocatalyst by immobilizing fungal laccase on the surface of yeast cells using synthetic bi

227 lbenzothiazoline-6-sulfonic acid) (ABTS) and Laccase on Vulcan XC-72, which act as a redox mediator,

228 ygen reduced in biocathode using immobilized laccase or bilirubin oxidase in order to generate suffic

229 he sensing system has a biocathode made from laccase or bilirubin oxidase, and the anode is made from

230 acid can be polymerized by biocatalysis with laccase or horseradish peroxidase.

231 effect of the shell cross-linking ability of laccase, or CaCl2, on microcapsules.

232 Secreted basidiomycete white-rot fungal laccases orchestrate this with high thermodynamic effici

233 te constant for the simultaneously occurring laccase-oxygen reaction is found to be 2.4 x 10(5) s(-1)

234 Using a set of congenic laccase-positive (2E-TUC-4) and laccase-deficient (2E-TU

235 Infection with laccase-positive (melanotic) C. neoformans cells also el

236 Free laccase produced cross linked water-insoluble oligomer,

237 water-insoluble oligomer, whereas conjugated laccase produced linear water-soluble oligomer.

238 ction pathways (GPA1, PKA1, PKR1, and RAS1), laccase production (LAC1), and the alpha mating type.

239 Virus-mediated suppression of laccase production was not affected by p40 deletion.

240 nd protein expression were somewhat reduced, laccase protein was found to be successfully translated

241 Treatment of RB221 with immobilized laccase reduced its toxicity up to 5.2%.

242 e novel molecular mechanisms addressing Pkc1-laccase regulation by the sphingolipid pathway of C. neo

243 involved in the regulation and expression of laccase remain largely unknown in C. neoformans.

244 Apo-laccase restoration by copper was found to be facilitate

245 The type 1 site of a plant laccase (Rhus vernicifera) is reduced moderately slowly

246 biocatalyst, referred to as surface display laccase (SDL), had an enzyme activity of 104 +/- 3 mU/g

247 Of the total 44, 40 laccases showed expression during different stages of fi

248 stinguish a fungal ferroxidase from a fungal laccase since the specificity that Fet3p has for Fe(II)

249 delignification of woody biomass by a small laccase (sLac) from Amycolatopsis sp.

250 meostasis in Escherichia coli, and the small laccase (SLAC) from Streptomyces coelicolor.

251 domains fused to a polyphenol oxidase, small laccase (SLAC).

252 The present experiments using laccase strongly support this view and suggest this reac

253 lethylamine, and a variety of peroxidase and laccase substrates, as well as carcinogenic benzidines,

254 ed covalent bond formation in the absence of laccase, suggesting a higher reactivity of their quinone

255 osa azurin, poplar plastocyanin, C. cinereus laccase, T. ferrooxidans rusticyanin, and human cerulopl

256 rotected phenols by employing a biocatalyst (laccase), tBuOOH, and either the Langlois' reagent or Ba

257 In contrast, oxidation by laccase tended to decrease the antioxidant capacity of r

258 analytical usefulness of the combined use of laccase, terbium oxide nanoparticles (Tb4O7NPs) and 8-hy

259 e (NBAD), a substrate for the phenol oxidase laccase that catalyzes the synthesis of cuticle protein

260 Furthermore, the expression of other laccases that are not predicted targets for known microR

261 For some of these laccases, the regulation was disrupted in a microRNA mat

262 escribe the recent burgeoning of prokaryotic laccases, their catalytic properties, structural feature

263 Unlike laccases, these BODs are stable in physiological conditi

264 However, in the case of C. cinereus laccase, this increase is attenuated by the presence of

265 conducted with type 1-substituted Hg (T1Hg) laccase to Fet3p and a mutant of Fet3p in which the trin

266 The presence of a hydrolyzable bond linking laccase to the cell wall was suggested by removal of lac

267 Special focus is given to the application of laccases to the emerging cellulosic biofuel industry.

268 In the present studies, we characterized laccase transcription and protein production to obtain i

269 laccase expression in the clc-a mutant was a laccase transcriptional defect that could be restored by

270 Laccase transcriptional induction by copper was found to

271 Furthermore, laccase treated juice displays an improved sensory profi

272 osinase increased their antioxidant activity laccase treatment resulted in a decreased activity and a

273 essed at normal levels or even higher in the laccase triple mutant, suggesting that lignin laccase ac

274 n AuNPs, fullerenols and Trametes versicolor Laccase (TvL) assembled layer by layer onto a gold (Au)

275 ic coupling and average distance between the laccase type-1 active site and the cathode substrate.

276 sed biosensor, a sentinel platinum sensor, a laccase/tyrosinase-based biosensor and a sentinel carbon

277 attenuated in virulence phenotypes including laccase, urease and growth under oxidative/nitrosative s

278 ssion of a number of virulence factors, i.e. laccase, urease and phospholipase.

279 e in a number of virulence factors including laccase, urease as well as soluble polysaccharide and de

280 co-activator of the fungal virulence factor, laccase, via binding to a GC-rich element within the 5'-

281 Laccase was added to the double-layer emulsions to coval

282 Laccase was detected in the fungal cell cytoplasm, cell

283 In the present study, laccase was expressed in C. neoformans lac1Delta cells a

284 ls at 0 degrees C, suggesting that cell wall laccase was expressed in the mutant as an apo-enzyme.

285 The direct electrochemical behavior of laccase was investigated using laccase immobilized diffe

286 ell-defined redox couple of Cu(I)/Cu(II) for laccase was observed at laccase immobilized GR-CMF compo

287 lectron-transfer from an N-hydroxy moiety to laccase was significantly affected by the redox potentia

288 First, the commercial laccase was ultrafiltrated allowing for the elimination

289 In addition, a Mexican endemic Laccase was used as the biocathode electrode and evaluat

290 Then, kinetic parameters of this laccase were determined for both substrates (FA, EF), in

291 lanin biosynthesis gene, LAC1, which encodes laccase were identified, and the T-DNA was shown to have

292 reeze-quench technique from Rhus vernicifera laccase when the fully reduced form reacts with dioxygen

293 actions is then extended to Rhus vernicifera laccase where a number of well-defined species including

294 es and molecular evolution, vis-a-vis fungal laccases where possible.

295 ins at least seven genes predicted to encode laccases, whereas the P. chrysosporium genome contains n

296 By combining laccase with catalase enzymes electrophoretically deposi

297 support for the cross-linking of the enzyme laccase with glutaraldehyde to construct a voltammperome

298 is and EPR spectroscopy during turnover of a laccase with quercetin; this species is assigned as a qu

299 sp., that can produce extracellular forms of laccases with an activity of approximately 58 300 U/L.

300 ciated with a partial mislocalization of GFP-laccase within cytosolic vesicles.

301 modification and their collective impact on laccase yields.