ライフサイエンスコーパス: lactalbumin

1 n the degradation of a model substrate (125I-lactalbumin).

2 series of proline variants of [28-111] alpha-lactalbumin.

3 d dynamics of native and the denatured alpha-lactalbumin.

4 plex of alphaB-crystallin and unfolded alpha-lactalbumin.

5 onances of the molten globule state of alpha-lactalbumin.

6 son and Kim for the homologous protein alpha-lactalbumin.

7 containing only residues 101 to 111 of alpha-lactalbumin.

8 the reduced, carboxymethylated form of alpha-lactalbumin.

9 meability of endothelial monolayers to alpha-lactalbumin.

10 evident for 125I-alpha-globin and 125I-alpha-lactalbumin.

11 e native main chain fold of apo bovine alpha-lactalbumin.

12 es derived from beta-lactoglobulin and alpha-lactalbumin.

13 oximately 1.3 times as high as that of alpha-Lactalbumin.

14 uration of the globular protein, human alpha-lactalbumin.

15 rom either high-isoflavone soy or casein and lactalbumin.

16 of bovine Gal-T1-Mn(2+)-UDP-GalNAc-Glc-alpha-lactalbumin.

17 of a single-disulfide variant of human alpha-lactalbumin ([28-111] alpha-LA) and of two mutants, each

18 four proteins, bovine S-carboxymethyl-alpha-lactalbumin (a disordered form of the protein with reduc

19 S-(Carboxymethyl)-alpha-lactalbumin, a disordered form of the protein with three

20 nized SWXJ mice with recombinant mouse alpha-lactalbumin, a lactation-dependent, breast-specific diff

21 Bovine alpha-lactalbumin, a small acidic Ca(2+)-binding milk protein,

22 alpha-Lactalbumin, a small calcium-binding protein, forms an e

23 termine whether the disulfide bonds of alpha-lactalbumin account for the lack of cooperative folding

24 reased from pH 7 toward pH 2, at which alpha-lactalbumin adopts a molten globule state, a small but i

25 ng degree of suppression of insulin or alpha-lactalbumin aggregation correlated with a decreasing Hsp

26 e whey (CW), beta-lactoglobulin (BLG), alpha-lactalbumin (ALA) and bovine serum albumin (BSA).

27 1) and type-3 (without calcium, ALA-3) alpha-lactalbumin (ALA) were examined.

28 nown bovine milk IgE-binding epitopes [alpha-lactalbumin (ALA), beta- and kappa-casein] and the corre

29 ly cationic lysozyme (Lys) and anionic alpha-lactalbumin (aLac), both of which can be found in the ci

30 By using alpha-lactalbumin, alcohol dehydrogenase, and insulin as targe

31 The pathway of oxidative folding of alpha-lactalbumin (alpha LA) (four disulfide bonds) has been c

32 alpha-Lactalbumin (alpha LA) forms a well-populated equilibriu

33 ng cow milk at 80 degrees C for 60min, alpha-lactalbumin (alpha-la) and beta-lactoglobulin (beta-lg)

34 Bovine alpha-lactalbumin (alpha-La) and kappa-casein (CN) displayed t

35 reversible thermal denaturation of apo alpha-lactalbumin (alpha-LA) and lysozyme was measured in the

36 milk at 80 degrees C for 60min, camel alpha-lactalbumin (alpha-la) and peptidoglycan recognition pro

37 e energy of unfolding (DeltaG(D-N)) of alpha-lactalbumin (alpha-LA) as a function of net charge.

38 scence properties of three variants of alpha-lactalbumin (alpha-LA) containing a single tryptophan re

39 The structure of denatured alpha-lactalbumin (alpha-LA) has been characterized using the

40 The molten globule state of alpha-lactalbumin (alpha-LA) has been considered a prototype o

41 Bovine alpha-lactalbumin (alpha-LA) has been shown by intrinsic prote

42 Recently, the molten globule of alpha-lactalbumin (alpha-LA) has been shown to adopt a native-

43 alpha-Lactalbumin (alpha-LA) is a two-domain calcium-binding p

44 Alpha-lactalbumin (alpha-LA) is one of the whey proteins in co

45 15N-1H HSQC NMR spectrum of the human alpha-lactalbumin (alpha-LA) molten globule at pH 2 and 20 deg

46 riant has been used as a model for the alpha-lactalbumin (alpha-LA) molten globule in a number of stu

47 dy the adsorption of Alexa 555 labeled alpha-lactalbumin (alpha-LA) on two chemically identical but m

48 ha), a two- disulfide variant of human alpha-lactalbumin (alpha-LA) that adopts a molten globule conf

49 identified calcium binding residues in alpha-lactalbumin (alpha-LA) was investigated by site-directed

50 ults showed that increased contents of alpha-lactalbumin (alpha-La) were associated with increased se

51 ed whey protein isolate (WPI) and pure alpha-lactalbumin (alpha-La) were investigated.

52 chain packing in the molten globule of alpha-lactalbumin (alpha-LA), a highly fluctuating, non-cooper

53 For human alpha-lactalbumin (alpha-LA), the equilibrium molten globule i

54 el of beta-lactoglobulin (beta-LG) and alpha-lactalbumin (alpha-LA).

55 s to a mammary gland-specific protein, alpha-lactalbumin (alpha-LA).

56 sly for the archetypal molten globule, alpha-lactalbumin (alpha-LA); this difference may be due to a

57 Electrostatic interactions between alpha-lactalbumin (alpha-lac) and carboxymethyldextran (CMD) i

58 ures of complexes made of the proteins alpha-Lactalbumin (alphaLA) and myoglobin (Mb) with the biosur

59 in the presence of a thiol initiator, alpha-lactalbumin (alphaLA) denatures by shuffling its four na

60 Four proteins, BSA, alpha-lactalbumin (alphaLA), lysozyme, and beta-lactoglobulin

61 Alpha-lactalbumin (alphaLA)-IIIA is a major kinetic intermedia

62 is a partially folded intermediate of alpha-lactalbumin (alphaLA).

63 s of the molten globule state of human alpha-lactalbumin (alphaLA).

64 olding and oxidative folding of bovine alpha-lactalbumin (alphaLA).

65 a-globin to approximately 85% for 125I-alpha-lactalbumin and 125I-albumin.

66 lobulin, lactoperoxidase, lactoferrin, alpha-lactalbumin and beta-lactoglobulin from sheep cheese swe

67 the bioactivities of proteins, such as alpha-lactalbumin and beta-lactoglobulin in vitro.

68 Immune-reactive alpha-lactalbumin and beta-lactoglobulin were found in the two

69 immunocytochemistry for milk proteins: alpha-lactalbumin and beta-lactoglobulin.

70 rotein isolates with varying ratios of alpha-lactalbumin and beta-lactoglobulin.

71 Proteins in the alpha-lactalbumin and c-type lysozyme family have been studied

72 pha-helical domain is observed in both alpha-lactalbumin and c-type lysozyme; however, the details of

73 nt expression of the milk protein gene alpha-lactalbumin and delayed expression of genes associated w

74 y identify the peak as a fragment from alpha-lactalbumin and eliminate a large number of spurious pro

75 sts of residues 20 through 36 of human alpha-lactalbumin and includes the entire B-helix.

76 Interestingly, lactalbumin and lactoferrin decreased hepatic lipidosis

77 Lactalbumin and lactoferrin decreased plasma leptin and

78 Dietary lactalbumin and lactoferrin improved energy balance and

79 Whey, lactalbumin and lactoferrin improved glucose clearance p

80 tein diets containing whey, or its fractions lactalbumin and lactoferrin, on energy balance and metab

81 ce in kinetic folding pathways between alpha-lactalbumin and lysozyme can be explained by the differe

82 oricidal complex of partially unfolded alpha-lactalbumin and oleic acid (OA).

83 reventing the amorphous aggregation of alpha-lactalbumin and the amyloid fibril formation of alpha-sy

84 tallin became entangled with unfolding alpha-lactalbumin and was a major portion of the resulting ins

85 A levels of endogenous MMTV as well as alpha-lactalbumin and whey acidic protein (WAP) were elevated.

86 The alpha-lactalbumins and c-type lysozymes have virtually identic

87 r proteins (bovine serum albumin, ovalbumin, lactalbumin) and four narrow fractions of Ficoll, were s

88 lactoglobulin A, beta-lactoglobulin B, alpha-lactalbumin, and albumin).

89 e, pyruvate kinase, albumin, lysozyme, alpha-lactalbumin, and beta-lactoglobulin.

90 ammaII-crystallin, myoglobin, barnase, alpha-lactalbumin, and cytochrome c the foldons and some nonco

91 f a single disulfide variant, [28-111] alpha-lactalbumin, and of a series of proline variants of [28-

92 f c-type lysozyme and one subfamily of alpha-lactalbumin are defined from 78 sequences, and their fol

93 Using insulin and alpha-lactalbumin as model target proteins, we also show that

94 We selected alpha-lactalbumin as our target vaccine autoantigen because it

95 ne the effects of two diets, one with casein-lactalbumin as the main protein source and the other wit

96 re fed diets that contained either 1) casein-lactalbumin as the source of protein (casein), 2) soy pr

97 t the levels of beta-lactoglobulin and alpha-lactalbumin associated with the casein micelles increase

98 ten globule state from measurements on alpha-lactalbumin at pH 2.0 over the temperature range 2 to 45

99 discovered for chordin-like protein 2, alpha-lactalbumin, beta-1,4-galactosyl transferase, and poly-I

100 imits of detection for model proteins, alpha-lactalbumin, beta-lactoglobulin A, and beta-lactoglobuli

101 ful OIT outcome, as high IgE levels to alpha-lactalbumin, beta-lactoglobulin and casein are associate

102 sing' the model formulas by increasing alpha-lactalbumin:beta-lactoglobulin enhanced heat stability a

103 In low alpha-lactalbumin:beta-lactoglobulin formulas, protein-protein

104 between proteins were minimal in high alpha-lactalbumin:beta-lactoglobulin formulas.

105 n-protein interactions with increasing alpha-lactalbumin:beta-lactoglobulin has important implication

106 5% protein) were formulated to contain alpha-lactalbumin:beta-lactoglobulin ratios of 0.1, 0.5, 1.3,

107 that increased heat stability in high alpha-lactalbumin:beta-lactoglobulin samples was due to decrea

108 nts indicate that reduced, carboxymethylated lactalbumin binds Hip with a Kd of 5 microM.

109 The internal dynamics of bovine alpha-lactalbumin (BLA) and its molten globules (MBLA) have be

110 Prolonged heating of holo bovine alpha-lactalbumin (BLA) at 80 degrees C in pH 7 phosphate buff

111 The refolding of bovine alpha-lactalbumin (BLA) from its chemically denatured state in

112 of the molten globule state of bovine alpha-lactalbumin (BLA) has been obtained using NMR spectrosco

113 +)-free apo form of recombinant bovine alpha-lactalbumin (BLA) is sufficiently stabilised in its nati

114 25I-labeled human alpha-globin, bovine alpha-lactalbumin, bovine serum albumin, or chicken lysozyme w

115 onsists of residues 72-100 from bovine alpha-lactalbumin, but with Cys 73, Cys 77, and Cys 91 replace

116 lower than the limit of detection and alpha-lactalbumin by approximately 34%, and was reduced furthe

117 hus, molten globule formation in human alpha-lactalbumin can be driven by the isolated alpha-subdomai

118 s as substrates (insulin-like growth factor, lactalbumin, casein, and alkaline phosphatase, whose len

119 f ribosomal protein L9 (CTL9), FKBP12, alpha-lactalbumin, colicin E7 immunity protein 7 (IM7), colici

120 alpha-Lactalbumin consists of two subdomains, and the alpha-su

121 ss structured and less stable than the alpha-lactalbumin construct.

122 ived from the alpha-subdomain of human alpha-lactalbumin containing the A, B, D, and 3(10) helices ar

123 of the low-pH molten globule of human alpha-lactalbumin, containing all four disulfides, has been ex

124 as its complex with the substrates and alpha-lactalbumin, crystallizes in a conformation (2.3A resolu

125 -two-state folding proteins, including alpha-lactalbumin, cytochrome c, intestinal fatty acid binding

126 Lactalbumin decreased weight and fat gain.

127 r to the molten globule state of human alpha-lactalbumin, demonstrates a marginal increase in the amo

128 ws that the heat capacity increment of alpha-lactalbumin denaturation highly correlates with the degr

129 sociation of an unassigned peak in the alpha-lactalbumin digest provided sufficient data to unambiguo

130 f R120G alphaB-crystallin to unfolding alpha-lactalbumin enhanced the kinetics and extent of its aggr

131 conditions than beta-lactoglobulin and alpha-lactalbumin, especially at longer drying times.

132 rom one another; the stronger one, apo-alpha-lactalbumin, exhibited a Kd of 35 microM.

133 structure, whereas the intermediate of alpha-lactalbumin exhibits the characteristics of a molten glo

134 ic for non-native domains since native alpha-lactalbumin fails to interact with Hip.

135 al features characteristic of a native alpha-lactalbumin fold.

136 The calcium binding protein alpha-lactalbumin folds via a molten globule intermediate.

137 was used to study the refolding of apo alpha-lactalbumin following dilution from guanidinium chloride

138 isplayed a strong immunoreactivity for alpha-lactalbumin for 2-96 h.

139 All alpha-lactalbumins form a well populated molten globule state,

140 sult, the denaturational transition of alpha-lactalbumin from the native to a highly ordered compact

141 Two different alpha-lactalbumin genetic constructs are studied: [28-111], wh

142 Although immunization with alpha-lactalbumin had no effect on fertility and birth numbers

143 s in stabilizing the molten globule of alpha-lactalbumin has been prepared.

144 cs of the molten globule (MG) state of alpha-lactalbumin have been characterized using (15)N transver

145 Structures of human alpha-lactalbumin have been generated with significantly diffe

146 rsus flat) of fibrils of lysozyme, apo-alpha-lactalbumin, HET-s (218-289) prion, and a short polypept

147 reement with previous studies on human alpha-lactalbumin (HLA) the unfolding process for BLA has been

148 oth cytochrome c and disulfide-reduced alpha-lactalbumin homodimers dissociate by a symmetrical charg

149 Similar results are observed for alpha-lactalbumin homodimers.

150 E3alpha-catalyzed conjugation to human alpha-lactalbumin, HsUbc2b-ubiquitin thiolester exhibits a K(i

151 non-native states of the protein human alpha-lactalbumin (human alpha-LA) with a detailed atomistic m

152 2, spectrin R16 domain, Arc repressor, alpha-lactalbumin, IM7, IM9, and spectrin R17 domain.

153 lity and birth numbers, pups nursed by alpha-lactalbumin-immunized mice showed significantly disrupte

154 The change in structure of bovine alpha-lactalbumin in environments of decreasing pH from pH 7 t

155 rin decreased plasma leptin and insulin, and lactalbumin increased peptide YY.

156 -crystallin in preventing the aggregation of lactalbumin induced by dithiothreitol.

157 ein mixture consisting of transferrin, alpha-lactalbumin, insulin, and alpha-2-macroglobulin.

158 Because alpha-lactalbumin is conditionally expressed only during lacta

159 nonnative structure for the folding of alpha-lactalbumin is discussed.

160 ing to residues 101-111 of the protein alpha-lactalbumin is remarkably structured in isolation in aqu

161 man carbonic anhydrase I, enolase, and alpha-lactalbumin) is achieved at 50- to 100-fold excess of th

162 n of a fusion protein comprising human alpha lactalbumin joined by an enterokinase cleavable linker t

163 using six proline variants of [28-111] alpha-lactalbumin, L11P, L12P, M30P, I95P, K108P and Q117P.

164 In addition, alpha-lactalbumin (LA) enhances this Glc-T activity more than

165 Under reducing conditions where alpha-lactalbumin (LA) unfolds and aggregates, XaHspA 36mers f

166 ta4Gal-T1) and a regulatory component, alpha-lactalbumin (LA), a mammary gland-specific protein.

167 Aromatic cluster 1 of alpha-lactalbumin (LA), a substructure adjacent to the cleft,

168 ubstrates and to the modifier protein, alpha-lactalbumin (LA), by over 99%.

169 In the presence of alpha-lactalbumin (LA), it transfers Gal to Glc, which is its

170 In the presence of alpha-lactalbumin (LA), the Gal acceptor specificity is altere

171 ndomized to isocaloric diets: Control, Whey, Lactalbumin, Lactoferrin, or pair-fed to lactoferrin.

172 cribes some of these proteins, such as alpha-lactalbumin, lactoferrin, osteopontin, and milk fat glob

173 ripts, encoding beta-casein (CSN2) and alpha-lactalbumin (LALBA), make up 45% of the total pool of mR

174 o the isolated alpha-helical domain of alpha-lactalbumin, Lyso-alpha with two native disulfide bonds

175 of hen lysozyme and a set of chimeric alpha-lactalbumin--lysozyme constructs.

176 HAMLET (human alpha-lactalbumin made lethal to tumor cells) is a tumoricidal

177 ordinary manner in which HAMLET (Human Alpha-lactalbumin Made LEthal to Tumor cells) kills a wide ran

178 in determining the overall fold of the alpha-lactalbumin molten globule.

179 However, in the case of alpha-lactalbumin more mass values were needed, and low mass e

180 y acidic protein mRNA was reduced, and alpha-lactalbumin mRNA was undetectable.

181 pha-catalyzed conjugation of the human alpha-lactalbumin N-end rule substrate shows Ubc2bS120D is 20-

182 The alpha-lactalbumins of members of the Camelidae family are the

183 oferrin (P < 0.01) and reduced that of alpha-lactalbumin (only at 90 min) (P < 0.05).

184 -lysozyme turnover, and decreased 125I-alpha-lactalbumin or 125I-albumin degradation by approximately

185 The ATP-dependent degradation of 125I-alpha-lactalbumin or 125I-albumin is probably mediated almost

186 in an 18-h ELISpot assay with casein, alpha-lactalbumin, or beta-lactoglobulin.

187 ad a larger increase in IgG4 levels to alpha-lactalbumin (P = 0.034), beta-lactoglobulin (P = 0.010),

188 IgE levels towards the milk allergens alpha-lactalbumin (P = 0.048), beta-lactoglobulin (P = 0.006)

189 ss structured and less stable than the alpha-lactalbumin peptide.

190 tructured than the corresponding human alpha-lactalbumin peptide.

191 ceiving soy diet premenopausally then casein/lactalbumin post-ovariectomy had higher relative hippoca

192 not fibrillate, although Zn(2+)-loaded alpha-lactalbumin precipitated out of solution as amorphous ag

193 Whey and lactalbumin produced transient hypophagia, whereas lacto

194 whey proteins, beta-lactoglobulin and alpha-lactalbumin, promoted colour change from blue to red for

195 n, the immobilized derivate hydrolyzed alpha-lactalbumin protein with a higher affinity than beta-lac

196 an milk but did affect lactoferrin and alpha-lactalbumin proteolysis and emulsion disintegration.

197 We found that immunoreactivity against alpha-lactalbumin provides substantial protection and therapy

198 f MCF7 cells with TDF led to production of a lactalbumin-related protein.

199 CKbeta4GT-I and CKbeta4GT-II encode an alpha-lactalbumin-responsive, UDP-galactose:N-acetylglucosamin

200 levels of MMTV, beta-casein, WAP, and alpha-lactalbumin RNA in virgin mammary glands compared to tho

201 Mice immunized with alpha-lactalbumin showed extensive T-cell-mediated inflammatio

202 mixture of bovine carbonic anhydrase, alpha-lactalbumin, soybean trypsin inhibitor, and ovalbumin wa

203 lidae family are the only of described alpha-lactalbumins that carry two AsnGly sequences.

204 lpy and entropy of the denaturation of alpha-lactalbumin to compact denatured state are always greate

205 Thus, alpha-lactalbumin vaccination may provide safe and effective p

206 , the percentage of positive cells for alpha-lactalbumin was significantly higher when compared with

207 e most active chaperone of insulin and alpha-lactalbumin was the Hsp27 (elongated) dimer, the smalles

208 A specific substrate of this pathway, alpha-lactalbumin, was ubiquitinated faster in the diabetic ex

209 lial marker CK14, and lactocyte marker alpha-lactalbumin were amongst the genes most highly expressed

210 The trypsin-digested spots of alpha-lactalbumin were analysed by MALDI-TOF mass spectrometry

211 rees C, whereas 218+/-43 mg/L residual alpha-lactalbumin were found at 130 degrees C.

212 ions with the highest concentration of alpha-lactalbumin were most stable to oxidation during storage

213 ding sites for the oligosaccharide and alpha-lactalbumin, when compared to the ligand-free structure.

214 e calcium-depleted form of the protein alpha-lactalbumin, which displays a cluster of acidic amino ac

215 The denatured states of alpha-lactalbumin, which have features of a molten globule sta

216 e very few spurious masses, and bovine alpha-lactalbumin, which under the conditions used, gave many

217 h norbixin than beta-lactoglobulin and alpha-lactalbumin, while kappa-casein bound with norbixin bett

218 the aggregation of reduced insulin or alpha-lactalbumin, with Hsp27 self-association as monitored by

219 dients, whey protein concentrate (WPC), whey lactalbumin (WLAC) and skim milk powder (SMP) on oat sta