ライフサイエンスコーパス: lactase

1 e lactose in milk more efficiently than free lactase.

2 parison with previous studies of sucrase and lactase.

3 75% for free lactase to<15% for encapsulated lactase.

4 embrane disruption to physically release the lactase.

5 s with genetic hypolactasia, explain the low lactase activities commonly found in malnourished infant

6 e in digestion and absorption was related to lactase activity (P=0.034, R2=0.38).

7 In vitro studies of intestinal lactase activity and breath-hydrogen studies have sugges

8 d yogurts appear to exhibit somewhat reduced lactase activity but are still well tolerated.

9 Lactase activity correlated with gestational age at birt

10 but in most mammals, including most humans, lactase activity declines after the weaning phase.

11 Lactose digestion and absorption and lactase activity doubled between studies (P=0.035 and P=

12 ldhood, but in European-derived populations, lactase activity frequently persists into adulthood.

13 Lactase activity is high and vital during infancy, but i

14 In other healthy humans, lactase activity persists at a high level throughout adu

15 es in lactose absorption relate primarily to lactase activity rather than to mucosal growth.

16 in was found only in adult animals (with low lactase activity), and there was no relationship between

17 elating to lactose digestion and absorption, lactase activity, and small-intestinal mucosal growth.

18 e directly lactose digestion and absorption, lactase activity, and small-intestinal surface area in p

19 dition of sodium caseinate further preserved lactase activity.

20 ourished controls with normal morphology and lactase activity.

21 glutaminase eliminated the detection of free lactase after freeze-drying emulsions and the addition o

22 are not detectable, despite the presence of lactase and phlorizin active sites in the polypeptide ba

23 Lactase and sucrase enzyme proteins and activities were

24 We see strong signals of selection at lactase and the major histocompatibility complex, and in

25 enterocyte integral membrane proteins using lactase as a prototype, and examined the possibility tha

26 There is little difference in the lactase capability of different commercial yogurts, beca

27 The levels of sucrase, maltase, and lactase decreased in wild-type mice p.i. with the GS str

28 Congenital lactase deficiency (CLD) is a rare severe autosomal rece

29 rst genetically confirmed case of congenital lactase deficiency in Central Europe.

30 Congenital lactase deficiency is an extremely rare gastrointestinal

31 This case demonstrates (a) that congenital lactase deficiency should be considered in cases of seve

32 ature termination of translation, congenital lactase deficiency was confirmed and intestinal biopsies

33 used watery diarrhoea, suggesting congenital lactase deficiency.

34 .01) and high IL-10 was correlated with high lactase dehydrogenase (P = .0085) and higher Internation

35 Simultaneously, cytotoxicity was assessed by lactase dehydrogenase leakage assay.

36 We sequenced the lactase enhancer region in 457 individuals from 18 Khois

37 hort tandem repeat (STR) loci that flank the lactase enhancer region.

38 5, which has been previously associated with lactase expression and lactose tolerance, had higher die

39 results from transcriptional suppression of lactase expression.

40 same LCT enhancer region can cause continued lactase expression.

41 s not, however, explain all the variation in lactase expression.

42 n induced a spurious association between the lactase gene (LCT) and tall/short status in a European A

43 n vivo transcriptional gradient of the mouse lactase gene (Lct), which occurs in enterocytes along th

44 Nuclear protein bound to the lactase gene cis element, CE-LPH1, was analyzed by elect

45 tence continue to express high levels of the lactase gene into adulthood.

46 To identify regions of the lactase gene involved in mediating the spatiotemporal ex

47 Transcription of the lactase gene is activated during enterocyte differentiat

48 acts as an enhancer to the expression of the lactase gene LCT is responsible for lactase persistence

49 riants was cloned upstream of the 3.0 kb rat lactase gene promoter in a luciferase reporter construct

50 after exclusion of primary LM by a negative lactase gene test.

51 Lactase gene transcription is spatially restricted to th

52 study concludes that malnutrition suppresses lactase gene transcription or mRNA stability in infants.

53 nal role for the polymorphisms in regulating lactase gene transcription.

54 most closely mimicked that of the endogenous lactase gene with respect to spatiotemporal restriction.

55 e 2, the most extreme signal is found in the lactase gene, which previously has been shown to be unde

56 variant in the cis-regulatory element of the lactase gene.

57 ide polymorphisms covering 3.2 Mb around the lactase gene.

58 d by a polymorphic element cis-acting to the lactase gene.

59 ome Diversity Project, and the analysis of a lactase-height dataset, we show that our method can corr

60 upstream from the start of transcription of lactase in an intron of the adjacent gene MCM6.

61 emulsions as delivery systems with retained lactase in milk and controlled release during in vitro d

62 re potential delivery systems to incorporate lactase in milk products.

63 Persistence of intestinal lactase into adulthood allows humans to use milk from ot

64 The persistent expression of lactase into adulthood in humans is a recent genetic ada

65 Synthesis of lactase is not affected by any of the cytokines.

66 Lactase is the intestinal disaccharidase responsible for

67 omization was applied in a subsample via the lactase LCT-13910 C/T single nucleotide polymorphism tha

68 A SNP in the gene encoding lactase (LCT) (C/T-13910) is associated with the ability

69 association between Bifidobacterium and the lactase (LCT) gene locus and identify an association bet

70 [polymorphism (rs4988235) upstream from the lactase (LCT) gene], where TT and TC genotypes are assoc

71 In malnourished infants, lactase messenger RNA (mRNA) was reduced to 32% and sucr

72 promoter and results in increased endogenous lactase messenger RNA.

73 The reductions of lactase mRNA, distinct from those found in adults with g

74 adequate to explain the greater reduction of lactase mRNA, this study concludes that malnutrition sup

75 nding the C_(13910) variant, associated with lactase non-persistence, results in a 2.2-fold increase

76 mL) per day produced negligible symptoms in lactase-nonpersistent (LNP) individuals self-described a

77 Bifidobacteria are associated with the human lactase nonpersister genotype, which typically confers l

78 Eastern Africa harbors distinctive lactase persistence (LP) alleles, and therefore LP allel

79 We genetically defined lactase persistence (LP) in 31 720 individuals from eigh

80 </= 20,089) evaluated associations between a lactase persistence (LP) SNP, the minichromosome mainten

81 intake maps closely onto the distribution of lactase persistence (LP), a genetic trait that allows mi

82 ery residues, faunal mortality profiles, and lactase persistence allele frequencies, provide a partia

83 n of the lactase gene LCT is responsible for lactase persistence and appears to have been under stron

84 here TT and TC genotypes are associated with lactase persistence and CC with nonpersistence.

85 3915 and C/G-13907) that are associated with lactase persistence and that have derived alleles that s

86 However, individuals with lactase persistence continue to express high levels of t

87 veness of our method on HapMap-simulated and lactase persistence datasets, where we significantly out

88 Lactase persistence has been strongly correlated with si

89 an evolutionary context for the emergence of lactase persistence in Africa.

90 ence) in Europeans, but the genetic basis of lactase persistence in Africans was previously unknown.

91 T, -13915T>G, -14010G>C) are associated with lactase persistence in different populations.

92 0,000 years, consistent with an advantage to lactase persistence in the setting of dairy farming; the

93 Lactase persistence is a heritable, autosomal dominant,

94 hat is frequent in Northern Europeans, where lactase persistence is frequent.

95 two alleles that are tightly associated with lactase persistence uniquely mark a common (~77%) haplot

96 ed in some cases, such as lactose tolerance (lactase persistence) in adults, but is less well underst

97 d with the ability to digest milk as adults (lactase persistence) in Europeans, but the genetic basis

98 er positive selection, including skin color, lactase persistence, and resistance to malaria.

99 These include those coding for lactase persistence, blue eye color, Y chromosome R1b ha

100 e, -14009*G, has borderline association with lactase persistence, but loses significance after correc

101 The T_(13910) variant, associated with lactase persistence, results in an even greater 2.8-fold

102 -obesity, observationally or genetically via lactase persistence.

103 n and surprisingly, no Neolithic presence of lactase persistence.

104 date) are each significantly associated with lactase persistence.

105 minantly inherited genetic trait is known as lactase persistence.

106 usative role in the mechanism specifying the lactase persistence/non-persistence phenotypes in humans

107 The DNA region of the C/T_(13910) lactase persistence/non-persistence variant functions in

108 enetic polymorphisms closely associated with lactase persistence/nonpersistence have been identified.

109 lactose into adulthood (i.e., they have the lactase-persistence [LP] trait).

110 However, a number of lactase persistent individuals carry none of these allel

111 the enhancer is significantly higher in the lactase persistent members of this and a second cohort c

112 o denied lactose intolerance (A-LNP), and 10 lactase-persistent individuals who believed they were la

113 The higher risk of type 2 diabetes in lactase-persistent individuals without milk intake likel

114 The distribution of these different lactase phenotypes in human populations is highly variab

115 r beta-glucosidase in the hydrolysis of PNG, lactase phlorizin hydrolase (LPH) was purified from rat

116 ture stop codons in the coding region of the lactase-phlorizin hydrolase (LPH) gene.

117 Lactase-phlorizin hydrolase (LPH) is an absorptive enter

118 liver fatty acid binding protein (Fabp1) and lactase-phlorizin hydrolase (LPH), and a surprising indu

119 ecline in production of the digestive enzyme lactase-phlorizin hydrolase during maturation.

120 PH1-binding proteins do not seem to regulate lactase-phlorizin hydrolase expression during this perio

121 Sucrase-isomaltase and lactase-phlorizin hydrolase expressions change remarkabl

122 e nuclear proteins to sucrase-isomaltase and lactase-phlorizin hydrolase gene expression in rats duri

123 to the CE-LPH1 cis-regulatory element of the lactase-phlorizin hydrolase gene, in this regulation.

124 hip between enzymatic activity and levels of lactase-phlorizin hydrolase mRNA.

125 r interruption of HNF-1-binding sites in the lactase-phlorizin hydrolase promoter resulted in a compl

126 necessary for cooperative activation of the lactase-phlorizin hydrolase promoter.

127 g because of decreasing levels of the enzyme lactase-phlorizin hydrolase, encoded by LCT.

128 Spray-dried lactase powder was suspended in anhydrous milk fat/Span(

129 Lactase promoter activities were assayed in cells transf

130 rsistence, results in a 2.2-fold increase in lactase promoter activity.

131 ve characterized the interaction between the lactase promoter and Cdx2, a homeodomain protein involve

132 homeodomain protein Cdx2 interacts with the lactase promoter and is capable of activating transcript

133 a previous report, Cdx2 interaction with the lactase promoter correlates with enterocyte differentiat

134 Thus, a distinct 5'-region of the lactase promoter directs intestine-specific expression i

135 erns of a luciferase reporter gene driven by lactase promoter regions in transgenic mice.

136 Two independent, 1.3- and 2.0-kb, lactase promoter-reporter transgenic lines expressed app

137 fferential transcriptional activation of the lactase promoter.

138 Many malnourished infants have reduced lactase specific activity in the small intestine.

139 Lactose digestion and absorption, lactase-specific activity, and lumen-to-mucosa water flu

140 8), located 13.9 and 22 kb upstream from the lactase structural gene.

141 Activities of lactase, sucrase, maltase, and palatinase consistently e

142 presence of functional brush-border enzymes (lactase, sucrase-isomaltase and dipeptidyl peptidase 4)

143 The bacterial lactase survives the acidic conditions of the stomach, a

144 shed children was associated with much lower lactase than sucrase mRNA abundance and because the epig

145 The enzyme lactase that is located in the villus enterocytes of the

146 ointestinal transit time allow the bacterial lactase to be active, digesting lactose from yogurt suff

147 ter 3-week storage reduced from>75% for free lactase to<15% for encapsulated lactase.

148 on of a neighboring gene (MCM6) and modulate lactase transcription in vitro.

149 localized to a 1.2-kb region upstream of the lactase transcription start site.

150 milk intake was 5 glasses/wk (IQR: 0-10) for lactase TT/TC persistence and 3 (0-7) for CC nonpersiste

151 Genetically for lactase TT/TC persistence compared with CC nonpersistenc

152 tinal Caco-2 cells were transfected with the lactase variant/promoter-reporter constructs and assayed

153 gion of the C/T_(13910) or G/A_(22018) human lactase variants was cloned upstream of the 3.0 kb rat l

154 The encapsulated lactase was released gradually during the simulated dige