ライフサイエンスコーパス: laminin 1

1 l three, but show decreased proliferation on laminin 1.

2 ndary and do not enter the region containing laminin 1.

3 D82 significantly inhibited cell adhesion on laminin 1.

4 gamma1) partially compensates for the absent laminin 1.

5 olecule in adhesion of a hemopoietic cell to laminin-1.

6 ediated through up-regulation of mesenchymal laminin-1.

7 peptide could inhibit tumor cell adhesion to laminin-1.

8 he pancreatic mesenchyme diffusely expresses laminin-1.

9 ind SV2 binds with high affinity to purified laminin-1.

10 is and is required for lamellae formation on laminin-1.

11 in cells adherent to thrombospondin-1 or to laminin-1.

12 myosin IIA or growth on high substrate-bound laminin-1.

13 eta4 integrin inhibited clone A migration on laminin-1.

14 sion of either subunit increased adhesion to laminin-1.

15 neither polymerized nor co-polymerized with laminin-1.

16 nd exhibits dynamic adhesion and motility on laminin-1.

17 LSIRT) decreases the size of acini formed on laminin-1.

18 also inhibited attachment to collagen IV and laminin-1.

19 dothelial marker, von Willebrand factor, and laminin-1.

20 ned the adhesion of prostate cancer cells to laminin-1.

21 otif sufficient to increase cell adhesion to Laminin-1.

22 ic sites in the extracellular matrix protein laminin-1.

23 growth cones turn on a uniform substrate of laminin-1.

24 ombospondin-2, and the E8 fragment of murine laminin-1.

25 ng alpha6beta1 integrin-mediated adhesion to laminin-1.

26 The disruption of the laminin layer by laminin-1 1) did not require the intact protein because

27 Further study demonstrated that exogenous laminin (1-10 microg/ml) treatment induced microglial ac

28 y of the following: 1) laminin (specifically laminin-1), 2) the "cross-region" of laminin-1, and 3) t

29 y distributed throughout the host, including laminins 1, 2, 4, 8, and 10.

30 h at least three different laminin isoforms (laminins 1, 2/4, and 10/11) in the blastocyst and in the

31 and a close self-assembly relationship among laminins-1, -2, and -4 were demonstrated by: (a) polymer

32 ules with either three full short arms (e.g. laminins-1-4), or molecules containing one (laminins-6-9

33 t could form laminin heterotrimers including laminins 1, 5, 10, and 11.

34 le into at least seven heterotrimers (called laminins 1-7).

35 at the decrease in apoptosis is dependent on laminin 1, a ligand for surface GalT I, suggesting that

36 ation of IGF-II levels increases adhesion to Laminin-1, a basement membrane protein down-regulated in

37 The laminin alpha1 chain is a subunit of laminin-1, a heterotrimeric basement membrane protein.

38 ked for interaction between purified SV2 and laminin-1, a laminin isoform with a similar structure.

39 Laminin-1, a major component of basement membranes, cons

40 Laminin-1, a multifunctional glycoprotein of the basemen

41 n of all the neurites, whereas inhibition of laminin-1 activity reduced the elongation of minor neuri

42 ing both peptide affinity chromatography and laminin-1 affinity chromatography.

43 oA(2-SO4) --> GlcNSO4] were also isolated by laminin-1 affinity chromatography.

44 e oligosaccharides were chromatographed on a laminin-1 affinity column.

45 d: discontinuous epithelial BM straining for laminin-1 (alpha 1 beta 1 gamma 1), entactin/nidogen and

46 antibodies directed against beta 2 laminin, laminin-1 (alpha 1-beta 1-gamma 1), and von Willebrand f

47 The best characterized laminin (laminin 1 = alpha 1, beta 1, gamma 1) promotes neurite o

48 Laminin (laminin-1; alpha 1-beta 1-gamma 1) is known to promote m

49 ed from the COOH-terminal globular domain of laminin-1 alpha1 chain (laminin G peptides), designated

50 of the laminin alpha2-chain, as well as its laminin-1 alpha1-chain counterpart, to identify candidat

51 s involved in neuronal viability, we infused laminin-1 (alpha1,beta1,gamma1) into the mouse hippocamp

52 inin heterotrimers: they grow freely between laminin 1 (alpha1beta1gamma1) and laminin 2, fail to cro

53 Laminin 1 (alpha1beta1gamma1) is a major laminin found a

54 previously identified 20 angiogenic sites on laminin-1 (alpha1beta1gamma1) by screening 559 overlappi

55 Fibulin-2 was also found to interact with laminin-1 (alpha1beta1gamma1) through a region (residues

56 Laminin-1 (alpha1beta1gamma1), self-assembles through a

57 Laminin 1 also affects cell-cell adhesion through change

58 In contrast to agrin, laminin-1 also does not induce tyrosine phosphorylation

59 twork formation and NIH3T3 cell spreading on laminin-1 (an alpha6beta1 ligand).

60 beta 1 is the major trophoblast receptor for laminin 1 and a functional receptor for laminins 2/4 and

61 ophic factor and its receptor as well as for laminin 1 and alpha6beta1 integrin.

62 a1 was capable of promoting motility on both laminin 1 and laminin 2/4 substrates.

63 Laminin 1 and laminin beta2 expression was maintained in

64 issues also expressed vimentin, collagen IV, laminin 1 and laminin beta2, whereas fibronectin was det

65 ants revealed that alpha7beta1 binds well to laminin 1 and to a mixture of laminin 2 and 4 but not to

66 become single cells or very small groups on laminin 1 and VE-cadherin localization at regions of cel

67 alpha chain, which is alpha1 and alpha2 for laminin-1 and -2, respectively.

68 The effects of laminin-1 and agrin are strictly additive and occur with

69 generation was induced by a chase with mouse laminin-1 and alpha2-macroglobulin.

70 genesis appears to require basement membrane laminin-1 and an alpha6-containing integrin receptor; 2)

71 TAG-1 and the extracellular matrix molecules laminin-1 and chondroitin sulfate proteoglycans (CSPGs)

72 servations suggest that transcripts encoding laminin-1 and collagen (IV) are increased in the colon a

73 a basement membrane, which we show contains laminin-1 and collagen IV.

74 library to identify sequences which bind to laminin-1 and elute with heparan sulfate or peptide 11 (

75 The processes differentially contribute on laminin-1 and fibronectin due to selective actin tetheri

76 ffect was mediated in part by an increase in laminin-1 and fibronectin mRNA levels and in part by the

77 Endothelial cells bind laminin-1 and form capillary-like structures when plated

78 can-1 binding site in the globular domain of laminin-1 and laminin-2.

79 s suggest that fibulin-2 functions to bridge laminin-1 and laminin-5 with other extracellular matrix

80 ivity and functional relationship within the laminin-1 and LG4 as well as the functional relation bet

81 kDa skeletal muscle alpha-dystroglycan, both laminin-1 and merosin binding to 120-kDa brain alpha-dys

82 Dual stimulation with laminin-1 and NRG1beta does not synergistically increase

83 that LG4 has a unique function distinct from laminin-1 and suggest that laminin alpha1 LG4-5 may also

84 which involves the cell-binding domain(s) of laminin-1 and tumor cell surface heparan sulfate (HS).

85 The AAb did not react with EHS laminin-1 and type IV collagen, pepsinized human type IV

86 ation in C2C12 myotubes, which suggests that laminin-1 and V. villosa agglutinin do not compete for t

87 In addition, dynamic patterns of laminins 1 and 10/11 expression in the embryo and the ut

88 We show here that laminins 1 and 10/11 have distinct effects on trophoblas

89 Laminins-1 and -2 are both composed of beta1 and gamma1

90 The present study shows that whereas laminins-1 and -2 are synthesized in the mouse developin

91 upportive if III(7-10), supportive if pFN or laminin-1) and suggest that efficient interaction of fre

92 The ECM proteins fibronectin, laminin 1, and collagen IV supported maximal cell adhesi

93 d laminin 2, fail to cross from laminin 4 to laminin 1, and stop upon contacting laminin 11.

94 fically laminin-1), 2) the "cross-region" of laminin-1, and 3) the alpha6 moiety of the integrin rece

95 RNA-7 decreased Caco2-BBE cell attachment on laminin-1, and CD98 overexpression recovered this inhibi

96 s were considerably more spread on Matrigel, laminin-1, and collagen I than the mock transfectants an

97 Static cell adhesion to laminin-1, and detachment of beads coated with fibronect

98 f fibronectin, heparan sulfate proteoglycan, laminin-1, and entactin was observed in the GBM of the a

99 local loss of beta1C integrin, of its ligand Laminin-1, and of IGF-II in the tumor microenvironment m

100 hesion to the matrix components fibronectin, laminin-1, and thrombospondin-1 differentially regulates

101 DWS repeats, is involved in cell adhesion to laminin-1, and we specifically implicate the repeat sequ

102 , whereas those spared intimate contact with laminin-1 appeared to organize into islets.

103 ated that MT was increased some 5-10-fold by laminin-1 as early as 6 h after incubation.

104 Laminin-6 failed to co-aggregate with laminin-1 at all concentrations evaluated, evidence for

105 tation identified that SPARC associates with laminin-1 before laminin secretion.

106 idly phosphorylated on S518 by Pak following laminin-1 binding to beta1 integrin, and by protein kina

107 We recently observed that laminin-1 binding to skeletal muscle alpha-dystroglycan

108 solid phase microtiter assay, 125I-laminin (laminin-1) bound to purified alpha-dystroglycan in a spe

109 s exhibit increased migration in response to laminin 1 but not to type-I or type-IV collagen.

110 fect that was overcome by co-adsorbed pFN or laminin-1 but not by soluble FN.

111 These peptides inhibited cell attachment to laminin-1 but not to collagen I, suggesting these active

112 icrog of peptide and inhibited attachment to laminin-1 but not to plastic or fibronectin.

113 the same low concentrations, only Drosophila laminin-1, but neither recalphaL nor recalphaS supported

114 ibited cardiac alpha-dystroglycan binding to laminin-1, but not to merosin.

115 )-neutralizing Ab 6A11 inhibited adhesion to laminin-1 by 98% (p < 0.05), demonstrating a novel role

116 alpha1 and laminin beta1, the remaining two laminin 1 chains.

117 e regeneration occurred within 6 h after the laminin-1 chase by forming a morphologically complete ba

118 agated in 3-dimensional cultures composed of laminin-1, collagen I, or mixtures of the two, and analy

119 es and to E3, an elastase digest fragment of laminin-1 containing AG73, is specific, since other lami

120 The G-domain of laminin-1 contains both integrin and heparin binding sit

121 hypothesis that the increased expression of laminin-1 contributes to the failure of crest-derived ce

122 tained in muscle; however, in pathway cells, laminin 1 declined modestly, and laminin beta2 decreased

123 Here we demonstrate that laminin-1 decreases methylation of the E-cadherin promot

124 trength of alpha6beta1-dependent adhesion to laminin-1, defined forces (0-1.5 nN) were applied to mag

125 in neurite outgrowth and also suggests that laminin-1 degradation by plasmin contributes to the proc

126 ull lens capsules exhibited higher levels of laminin-1 deposition relative to their wild-type counter

127 Laminin-1 derivatized plates were used for biopanning.

128 did not induce capillary morphogenesis, and laminin-1 did not induce activation of Src or Rho.

129 In sharp contrast, laminin-1 did not induce capillary morphogenesis, and la

130 Laminin-1 does not cause tyrosine phosphorylation of MuS

131 ainst alpha6 integrin, beta1 integrin or the laminin-1/E8 domain recognized by alpha6beta1 integrin,

132 Moreover, pre-exposure to anti-laminin-1/E8 function-blocking antibody prevented reorie

133 thin 1 day of plating P19 embryoid bodies on laminin-1 (EHS laminin).

134 nents (type IV, collagen, perlecan, bamacan, laminin-1, entactin-nidogen, fibronectin) in SEF areas a

135 It binds collagen IV, laminin-1, entactin/nidogen-1, fibronectin and vitronect

136 n critical concentration, co-aggregated with laminin-1, evidence for co-polymerization.

137 The pattern of laminin-1 expression in the embryonic pancreas supports

138 Since intact laminin-1 failed to trigger these events, we hypothesize

139 cells cultured with and without TGF-beta1 to laminin-1, fibronectin, and vitronectin along with expre

140 e ability of an LCMV isolate to compete with laminin-1 for receptor binding is determined by its bind

141 Elastase-generated laminin-1 fragments were fractionated by heparin-Sepharo

142 Here we report that, in Xenopus, laminin-1 from the extracellular matrix (ECM), converts

143 east partially due to the interaction of the laminin-1 gamma1 chain with endogenous laminin-10, becau

144 protein because infusion of plasmin-digested laminin-1 gave similar results; 2) was posttranscription

145 ere we demonstrate that elastase cleavage of laminin-1 generates fragments, which stimulate proteinas

146 1-chain (SRARKQAASIKVAVSADR), whereas intact laminin-1 has no effect on proteinase expression by macr

147 sequences that are necessary for binding to laminin-1 have not been characterized.

148 and hence other isoforms, act redundantly to laminin 1 in posterior notochord and ISV development.

149 These effects, and the localization of laminin 1 in Reichert's membrane and laminin 10/11 in th

150 lular beta1 integrin ligands fibronectin and laminin-1 in a cohort of 249 breast cancer patients who

151 Furthermore, increased laminin-1 in lens capsule promoted the attachment of len

152 This study determines the role of laminin-1 in promoting metastatic colonization during br

153 nces between the functions of collagen I and laminin-1 in regulating endothelial cell morphogenesis.

154 We first defined the ontogeny of laminin-1 in the developing mouse pancreas.

155 Because abnormal deposition of laminin-1 in the lens BM could influence lens epithelial

156 xtracellular matrix proteins fibronectin and laminin-1 in these cells.

157 larger acinar-like structures on exposure to laminin-1 in vitro and smaller, more differentiated tumo

158 E3 or laminin-1 increase Grb2-binding and Rac1 activation.

159 ion of PMA-stimulated cultured mast cells to laminin-1 increased from 5.3 +/- 3.6% (mean +/- SEM) in

160 Here, microarray analysis showed that laminin-1 induced mainly one gene family of proteins, th

161 src and fyn and found that neural agrin and laminin-1 induced normal clustering of AChRs and that ag

162 Rather, laminin-1 induced persistent activation of the GTPase Ra

163 Most importantly, laminin- 1-induced clustering does not require MuSK, a r

164 nhibited agrin-, V. villosa agglutinin-, and laminin-1-induced acetylcholine receptor clustering in C

165 Moreover, laminin-1 induces AChR clustering by a pathway that is i

166 of human submandibular gland (HSG) cells on laminin-1 induces acinar differentiation.

167 In the presence of collagen, laminin-1 inhibited induction of MMP-1 but laminin-5 did

168 To control for specificity of laminin-1 interactions, cells were also cultured on 2-di

169 Infusion of plasmin-digested laminin-1 into the hippocampus of lamgamma1 or tPA KO mi

170 In the implanting blastocyst, laminin 1 is strongly expressed in the trophectoderm bas

171 Laminin-1 is a basement membrane glycoprotein implicated

172 -dimensional model, we present evidence that laminin-1 is capable of inducing epigenetic change by in

173 Laminin-1 is composed of 3 chains, alpha1, beta1, and ga

174 , where axons turn sharply to leave the eye, laminin-1 is confined to the retinal surface.

175 Laminin-1 is essential for early embryonic basement memb

176 ryonic pancreas supports the conclusion that laminin-1 is important in the induction of exocrine (duc

177 Because laminin-1 is known to contain multiple biologically acti

178 Basement membrane laminin (laminin-1) is a multidomain glycoprotein that interacts

179 Previous studies demonstrated that laminin 1 (L1) is critical for intact salivary cell clus

180 on proteoglycans and was more effective than laminin-1, L1-Fc, or intact tenascin-C, thus demonstrati

181 eptide for blocking adhesion to fibronectin, laminin 1, laminin 5, and collagen IV was 2.4 microg, 1.

182 eptide for blocking adhesion to fibronectin, laminin 1, laminin 5, and collagen IV was 6.9 microg, 5.

183 ound to laminin-5 and laminin-10, but not to laminin-1, laminin-2, fibronectin, various collagens, ni

184 ttach to and extend neurites on substrata of laminin-1 (LN-1) during late embryogenesis, in a time fr

185 minins' functions, we examined the motion of laminin-1 (Ln-1) in physiological buffers using atomic f

186 urons lose the ability to extend neurites on laminin-1 (LN-1) with increasing developmental age yet s

187 eurites on the extracellular matrix molecule laminin-1 (LN-1), despite the fact that they retain expr

188 f activated Rac1, or the inclusion of excess laminin-1 (LN-1).

189 ases the adhesive strength of these cells on laminin-1 matrices, although it does not increase their

190 Thus, laminins (but not laminin 1) may promote peripheral axonal outgrowth.

191 Adhesion to laminin-1 mediated by the alpha6Abeta1 integrin triggere

192 he most potent gamma1 chain peptide, blocked laminin-1-mediated adhesion and was the only gamma1 chai

193 In contrast, laminin-1-mediated fibroblast attachment and spreading w

194 aring the distribution of agrin with that of laminin-1, merosin (laminin-2), neurofilament, and neura

195 (KQNCLSSRASFRGCVRNLRLSR) in the G domain of laminin-1, modeled as a right-handed alpha-helix, carrie

196 In contrast to collagen I, laminin-1 neither suppressed cAMP nor protein kinase A a

197 hR clustering in C2 myotubes is specific for laminin-1; neither laminin-2 (merosin) nor laminin-11 (a

198 e blockage of duct morphogenesis by the anti-laminin-1 neutralizing antibodies.

199 On laminin-1, NGF induced greater vinculin-dependent adhesi

200 lamina proteins from chick embryos, such as laminin-1, nidogen-1, collagens IV and XVIII, perlecan,

201 In contrast, neither intact laminin-1 nor the beta1-chain peptide CDPGYIGSR stimulat

202 ed NC1 to laminin 5-coated wells, but not to laminin 1 or albumin.

203 isruption of the endogenous laminin layer by laminin-1 or anti-laminin gamma1 antibody renders the tP

204 forces exerted through alpha6beta4 on either laminin-1 or on an anti-alpha6 antibody, demonstrating t

205 -mediated adherence to a preformed matrix of laminin-1 or pFN to support assembly of FN.

206 ivation of integrin receptors by adhesion to laminin-1 or Semaphorin 7A also promotes neurite branchi

207 ue for carcinoma cells migrating randomly on laminin-1 or stimulated to migrate on collagen I with ly

208 ophin is increased by the addition of either laminin-1 or the isolated laminin alpha1 globular domain

209 amount of cAMP decreases in the presence of laminin-1 or YIGSR, suggesting a possible mechanism for

210 nhibited adhesion of MDA-MB-435 cells to the laminin-1 peptide GD6, which contains a potential integr

211 High substrate-bound laminin-1 prevents or reverses these changes.

212 Laminin 1 promotes random migration and decreases spread

213 is revealed increased sinusoidal PECAM-1 and laminin-1 protein expression, suggesting gain of adheren

214 PARC affects the secretion and deposition of laminin-1 protein in lens epithelial cells.

215 An uneven and aggregated distribution of laminin-1 protein was apparent in the anterior region of

216 nt membrane and, in particular, contact with laminin-1 provides a potent signal to down-regulate MMP-

217 , the chemotactic migration of A431 cells on laminin-1 requires not only the formation of F-actin-ric

218 trimers exert distinct effects on subsets of laminin-1-responsive cells, suggesting that isoform dive

219 me-lapse videomicroscopy of clone A cells on laminin-1 revealed that their migration is characterized

220 m capillary-like structures when plated on a laminin-1-rich basement membrane matrix, Matrigel.

221 rentiate into capillary-like structures on a laminin-1-rich matrix (Matrigel).

222 ses, epithelial cells in direct contact with laminin-1 seem to differentiate into ducts and acini, wh

223 all three proteins was inhibited by EDTA and laminin-1 short arm fragments, (b) polymerization of lam

224 either chain results in the dramatic loss of laminin 1 staining throughout the embryo and prevents fo

225 ion and migration of alpha7 transfectants on laminin 1 substrates.

226 adhered with high efficiency and migrated on laminin 1 substrates.

227 differences in cell adhesion or motility on laminin 1 substrates.

228 In the presence of E3 or laminin-1, syntrophin is phosphorylated on a tyrosine re

229 cell attachment and inhibiting attachment to laminin-1 than either C16 or C16Y.

230 components (e.g., growth factors) other than laminin-1 that can sustain both carbonic anhydrase II ex

231 trate that plasminogen specifically bound to laminin-1, the interaction resulted in increased plasmin

232 integrin functions in carcinoma migration on laminin-1 through its ability to promote the formation a

233 Laminin-1 thus acts by a mechanism that is independent o

234 th alpha1:SRARKQAASIKVAVSADR, but not intact laminin-1, triggers protein kinase C-dependent activatio

235 , a peptide derived from the alpha1-chain of laminin-1, triggers protein kinase C-dependent activatio

236 espite such differences in binding, LCMV and laminin-1 use, in part, an overlapping binding site on a

237 anism that locally supports cell adhesion to Laminin-1 via IGF-II is selectively regulated by the bet

238 ion and survival of HPPL in culture, whereas laminin-1 was a crucial component of Matrigel for induci

239 Increased adhesion of MMC homologues to laminin-1 was also stimulated by FcepsilonRI cross-linki

240 Moreover, during NGF-induced neuritogenesis, laminin-1 was degraded, and this cleavage was catalyzed

241 The laminin-1 was essential in reconstituting a new basal la

242 Laminin-1 was found previously to promote the morphologi

243 1 short arm fragments, (b) polymerization of laminin-1 was inhibited by fragments of laminins-2 and -

244 ng at the marginal edges of clone A cells on laminin-1 was resistant to solubilization with Triton X-

245 SPARC and laminin-1 were expressed abundantly in the endoplasmic r

246 e alpha-, beta-, and gamma-chain subunits of laminin-1 were expressed in all combinations, transientl

247 concentrations of V. villosa agglutinin and laminin-1 were strictly additive with respect to acetylc

248 ast carcinoma cells, normally nonadherent to laminin 1, were stably transfected with cDNA for mouse a

249 ng the alpha 7-X2 variant adhered rapidly to laminin 1, whereas those expressing alpha 7-X1 failed to

250 a7X1 coupled with beta1A failed to adhere to laminin-1, whereas cotransfectants expressing alpha7X1 a

251 Intact laminin-1, which was unable to induce macrophage protein

252 A soluble peptide fragment of laminin-1 (YIGSR) mimics this laminin-induced conversion