ライフサイエンスコーパス: laminin 5

1 a 3 beta 1 with distinct ligand specificity (laminin-5).

2 biglycan, and the basement membrane protein laminin 5.

3 adhere to collagen but adhere and spread on laminin 5.

4 LAMB3 gene encoding the beta3 polypeptide of laminin 5.

5 , or the alpha3, beta3, and gamma2 chains of laminin 5.

6 ding mediated by integrin alpha(3)beta(1) on laminin 5.

7 binding site inhibited the binding of NC1 to laminin 5.

8 f NC1 was identified as the binding site for laminin 5.

9 ing the constitutive polypeptide subunits of laminin 5.

10 ta4 integrin complex, and the beta3 chain of laminin 5.

11 polypeptides (alpha3, beta3, and gamma2) of laminin 5.

12 biosynthetic processing of lysyl oxidase and laminin 5.

13 ligrin cicatricial pemphigoid sera recognize laminin 5.

14 ted, the RPE cells adhered preferentially to laminin 5.

15 RPE cells rapidly attached and flattened on laminin 5.

16 seen by degradation of type IV collagen and laminin 5.

17 regulates alpha3beta1-dependent migration on laminin 5.

18 gen type IV expression pattern and decreased laminin 5.

19 e a domain (DIII) from the ECM macromolecule laminin-5.

20 pha 3-dependent random CHO cell migration on laminin-5.

21 secrete an extracellular matrix deficient in laminin-5.

22 with most patients shown to have defects in laminin-5.

23 so immunolabeled BMZ of JEB skin that lacked laminin-5.

24 higoid immunoprecipitated both laminin-6 and laminin-5.

25 confirming the interaction of fibulin-2 with laminin-5.

26 levels of the beta4 integrin, which binds to laminin-5.

27 on but not adhesion on substrates other than laminin-5.

28 non-polarized spreading of keratinocytes on laminin-5.

29 n decreased cell adhesion to fibronectin and laminin-5.

30 3beta1 internalization in cells migrating on laminin-5.

31 response to epidermal growth factor (EGF) on laminin-5.

32 of IkappaB in beta4 mutant cells adhering to laminin-5.

33 membrane with upregulation of laminin-8 and laminin-5.

34 et PAK1 were activated following adhesion to laminin-5.

35 iates cellular adhesion to the matrix ligand laminin-5.

36 showed comparable expression and adhesion to laminin-5.

37 otein Shc when the cells are stimulated with laminin-5.

38 T84 cells express primarily laminins 5, 6, and 7 as indicated by immunostaining usin

39 the laminin alpha 3 subunit, a component of laminins 5, 6, and 7, completely inhibited the closure o

40 a2 cleavage is required for formation of the laminin 5-6 complex and that this complex is directly in

41 cally to fibronectin, collagen type I, and a laminin 5/6 complex.

42 udy, we examined the interaction of NC1 with laminin 5 (a component of anchoring filaments).

43 promotes expression of the precursor form of laminin 5, a characteristic of leading keratinocytes in

44 Furthermore, laminin-5, a common ligand for integrins alpha3beta1 and

45 e integrins alpha6beta4 and alpha3beta1 bind laminin-5, a component of basement membranes.

46 inocytes demonstrated decreased secretion of laminin-5, a laminin isoform known to inhibit keratinocy

47 Laminin-5, a major adhesive ligand for epithelial cells,

48 are abundant receptors on keratinocytes for laminin-5, a major component of the basement membrane be

49 In Herlitz junctional epidermolysis bullosa (laminin 5 abnormalities, n = 4) these values were reduce

50 ells displayed markedly impaired motility on laminin-5, accompanied by unusually persistent lateral a

51 more the data are consistent with a role for laminin-5, acting through its alpha3beta1 and/or alpha6b

52 Adhesion to laminin-5 activates extracellular signal-related kinase

53 an BCCs, including decreased BP180/BPAG2 and laminin 5 adhesion proteins and expression of basal epid

54 nogen activation, the globular domain of the laminin-5 alpha(3) subunit, a major pp126 matrix protein

55 the G(1) subdomain of the N terminus of the laminin-5 alpha(3) subunit, with equilibrium dissociatio

56 laminin-5 as a true ligand for alpha(3), the laminin-5/alpha(3)-dependent motility of ldlD/CD9 cells

57 exogenous tPA results in proteolysis of the laminin-5 alpha3 chain from 190 to 160 kD.

58 beta1gamma1), laminin-4 (alpha2beta2gamma1), laminin-5 (alpha3Abeta3gamma2), and laminin-6 (alpha3Abe

59 However, on laminin-5 (alpha3beta1 integrin ligand), A431 cell reagg

60 vates RhoA, slowing cell locomotion, whereas laminin-5-alpha3beta1 integrin interaction inhibits RhoA

61 ntaining low calcium proteolytically process laminin 5 (alpha3beta3gamma2) within the alpha3 and gamm

62 antibasement membrane autoantibodies against laminin 5 (alpha3beta3gamma2).

63 The gene LamC2 encoding the gamma2 chain of laminin 5, an epithelial cell-specific extracellular mat

64 s, and the polarized, linear distribution of laminin 5 and a6 integrin subunit.

65 Human cell tumorigenesis is dependent on laminin 5 and alpha6beta4 integrin.

66 ains showed distruption in the deposition of laminin 5 and an apparent lack of fibronectin at the edg

67 cleaves gamma2 in vitro, both within intact laminin 5 and at the predicted site of a recombinant gam

68 pithelial sheet showed negative staining for laminin 5 and collagen VII, but interrupted linear basal

69 TAT3-dependent manner, which also stabilizes laminin 5 and engages cells to form hemidesmosome-like j

70 lar matrix components including a complex of laminin 5 and laminin 6.

71 a(4) or laminin 5 confirm that deposition of laminin 5 and ligation by alpha(6)beta(4) are required f

72 Deposition of laminin 5 and ligation of alpha(6)beta(4) increases PI3K

73 A3 gene, which encodes the alpha3 subunit of laminin 5 and other isoforms, to examine developmental f

74 ristics in the basal epithelium by degrading laminin 5 and part of collagen IV, and disassembling col

75 ufficient to elevate expression of precursor laminin 5 and RhoGTP, allowing for subsequent collagen a

76 Immunoreactivity for both laminin 5 and Type IV collagen did not show any signific

77 rane formation was evaluated by detection of laminin 5 and Type IV collagen expressions on immunohist

78 this study is to evaluate the expression of laminin 5 and Type IV collagen histologically in regener

79 hMSC isolated from bone synthesize laminin-5 and adhere to exogenous laminin-5 through alph

80 Bead-immobilized laminin-5 and collagen I, two major alpha(3)beta(1) liga

81 ve studied the molecular interaction between laminin-5 and extracellular matrix proteins using recomb

82 ing antibody 9EG7, binding affinity for both laminin-5 and invasin increased by about 10-fold, wherea

83 cal for adhesion to invasin, indicating that laminin-5 and invasin may use different recognition mech

84 soluble alpha 3 beta 1 specifically bound to laminin-5 and laminin-10, but not to laminin-1, laminin-

85 Laminin-5 and other matrix proteins remained associated

86 analyses that demonstrate colocalization of laminin-5 and plasminogen in the extracellular matrix of

87 -cell adhesion and slow down haptotaxis over laminin-5 and point to the alpha6beta4-erbB2 heterodimer

88 s a critical role in tumor cell responses to laminin-5 and reveal promotion of integrin recycling as

89 Laminin-332 (formerly laminin-5) and collagen VII are basement membrane protei

90 rmal keratinocytes (containing laminin-6 and laminin-5) and JEB keratinocytes (containing laminin-6 b

91 blocking adhesion to fibronectin, laminin 1, laminin 5, and collagen IV was 2.4 microg, 1.8 microg, 4

92 blocking adhesion to fibronectin, laminin 1, laminin 5, and collagen IV was 6.9 microg, 5.7 microg, >

93 ncubation for collagen IV, collagen VII, and laminin 5, and through 5 wk for epidermal barrier by sur

94 ted negative immunofluorescence staining for laminin 5, and transmission electron microscopy revealed

95 timulation), 2) the shape of cells spread on laminin-5, and 3) alpha 3-dependent random CHO cell migr

96 did not alter cell adhesion or spreading on laminin-5, and had no effect on reaggregation of cells p

97 mbly are predicted to limit the secretion of laminin-5, and likely to interfere with function.

98 ng with ZO-1, connexin 43, type IV collagen, laminin-5, and perlecan, and apoptosis was determined by

99 Interleukin (IL)-1beta, laminin-5, and thrombospondin-1, which have been shown t

100 rane components type VII and IV collagen and laminin 5 antibodies.

101 midesmosome-specific alpha6beta4; both share laminin 5 as ligand.

102 ified recombinant NC1 bound to human and rat laminin 5 as measured by enzyme-linked immunosorbant ass

103 he epithelial basement membrane glycoprotein laminin-5 as a model to evaluate molecular events that d

104 Together, these data identify laminin-5 as a substrate for mTLD, suggesting a role for

105 (3)/CD9 interaction by GM3 and the status of laminin-5 as a true ligand for alpha(3), the laminin-5/a

106 In all six JEB patients, the laminin-5 assembly intermediates we observed were as pre

107 These studies demonstrate that human anti-laminin 5 autoantibodies are pathogenic in vivo and desc

108 m patients with alpha subunit-specific, anti-laminin 5 autoantibodies, or normal controls.

109 entrations that do not apparently effect the laminin-5 beta and gamma chains.

110 m mutations in the LAMB3 gene, which encodes laminin 5 beta3 (beta3).

111 pitopes within NC1 that colocalized with the laminin 5 binding site inhibited the binding of NC1 to l

112 k (Tyr-186 and Trp-188) or enhance (Asp-122) laminin-5 binding to alpha 3 beta 1 did not affect lamin

113 n-5 binding to alpha 3 beta 1 did not affect laminin-5 binding under the assay conditions used.

114 ly-163 residues in this predicted loop block laminin-5 binding.

115 When keratinocytes were adhered on laminin 5, both structural (assembly of connexin 43 in g

116 P-2 did not cleave the gamma2 chain of human laminin-5 but processed the rat laminin gamma2 chain to

117 C1 bound predominantly to the beta3 chain of laminin 5, but also to the gamma2 chain when examined by

118 e tail-less alpha6beta4 binds efficiently to laminin 5, but is unable to integrate with the cytoskele

119 Consistently, adhesion of quiescent HFKs to laminin 5, but not collagen, also promotes expression of

120 Chinese hamster ovary (CHO) cell adhesion to laminin-5, but did alter 1) alpha 3-dependent tyrosine p

121 Ligation of laminin 5 by integrin alpha(6)beta(4) activates phosphoi

122 The pp126 cell 190-kD alpha3 chain of laminin-5 can be specifically proteolyzed by plasmin to

123 Laminin 5 chains are not only considerably truncated wit

124 Protein and mRNA expression of all three laminin 5 chains were detected in the basal cells of nor

125 Antibodies against laminin-5 chains served to increase the number of strain

126 his patient and two other patients with anti-laminin-5 cicatricial pemphigoid immunoprecipitated both

127 y and by binding of 125I-radiolabeled NC1 to laminin 5-coated wells, but not to laminin 1 or albumin.

128 iv) Adhesion of HCV29 or YTS-1/CD82 cells to laminin-5-coated plate activated cMet kinase in the abse

129 (iv) Adhesion of YTS1 or YTS1/CD82 cells to laminin-5-coated plates, as compared with noncoated plat

130 Adhesion of keratinocytes on laminin 5, collagen, and fibronectin was found to differ

131 ial cells that could be rescued by exogenous laminin 5, collagen, or an antibody against integrin alp

132 The laminin-5 component of the extracellular matrices of cer

133 ith keratinocytes lacking alpha(6)beta(4) or laminin 5 confirm that deposition of laminin 5 and ligat

134 Epithelial cell-specific laminin-5, consisting of three chains, alpha3, beta3, an

135 We isolated the laminin 5 contained within the basement membrane of huma

136 Laminin-5 containing the 160-kDa alpha(3) subunit effici

137 in this report suggest that the decrease in laminin-5 content in hypoxic matrix, relative to matrix

138 esion of p300-overexpressing cells is due to laminin-5-deficient extracellular matrix and not due to

139 read poorly compared with wild-type cells on laminin-5, demonstrating a postattachment requirement fo

140 NC1 (FNC1) promoted tumor cell invasion in a laminin 5-dependent manner and were required for tumorig

141 ion and signaling from collagen-dependent to laminin 5-dependent.

142 Laminin-5-dependent Met activation was minimal in HCV29

143 Conversely, blocking laminin 5-deposition with brefeldin A, an inhibitor of v

144 , laminin-1 inhibited induction of MMP-1 but laminin-5 did not.

145 in), basement membrane assembly (Collagen 7, Laminin 5), differentiation (K13, K3), proliferation (Ki

146 ins found in the basement membrane including laminin-5, entactin, and perlecan.

147 on and immunohistochemical staining revealed laminin 5 expression mostly in the subcapsular region of

148 Resynthesis of BM began with laminin-5 followed by other components.

149 Cells plated on laminin-5 for 16 d express increased levels of osteogeni

150 Invasin completely displaced laminin-5 from the alpha 3 beta 1 integrin, suggesting s

151 In the absence of laminin 5 function, we were able to detect a new ligand

152 wt) alpha3 or point mutants unable to engage laminin 5 (G163A) or epithelial cadherin (E-cadherin; H2

153 2, MMP-2, membrane type 1-MMP (MT1-MMP), and laminin 5 gamma 2 (LN 5 gamma 2) in tumor cells correlat

154 perative interactions of MMP-2, MT1-MMP, and laminin 5 gamma 2 chain and, thus, the remodeling of the

155 antigen, vascular endothelial-cadherin, and laminin 5 gamma 2 chain domain III fragment in lymph nod

156 structures through a novel relationship with laminin 5 gamma 2 chain domain III fragments.

157 , inhibition of PI3K blocked the cleavage of laminin 5 gamma 2 chain, resulting in decreased levels o

158 like 2 (mTLL-2), inhibited the processing of laminin-5 gamma2 and alpha3 chains in keratinocyte cultu

159 survey, all BMP-1 isoenzymes processed human laminin-5 gamma2 and alpha3 chains to 105- and 165-kDa f

160 reater elastin degradation and proangiogenic laminin-5 gamma2 peptide production, which may account f

161 Mutations within laminin-5 genes were diversely located, with the most se

162 These results indicate that laminin 5 has an important role in regulating tissue org

163 Herlitz-JEB) of one of the chains needed for laminin-5 heterotrimer assembly.

164 on levels, and protein chain assembly of the laminin-5 heterotrimer in six JEB patients to determine

165 encoding the alpha3 and gamma2 chains of the laminin-5 heterotrimer in the cells that overexpress p30

166 hese regions coexpressed the gamma2 chain of laminin 5, identified previously as a marker of invasion

167 Anti-laminin 5 IgG also induced subepidermal blisters in: adu

168 dult BALB/C mice challenged with rabbit anti-laminin 5 IgG developed, in a concentration-related fash

169 hat received patient autoantibodies had anti-laminin 5 IgG in their circulation, deposits of human Ig

170 Although passive transfer of rabbit anti-laminin 5 IgG to neonatal mice has been shown to induce

171 and provides novel insight into the role of laminin 5 in keratinocyte biology.

172 suggest that adhesion of epithelial cells to laminin 5 in the basement membrane via alpha3beta1 promo

173 regulation of matrix metalloproteinase 1 and laminin 5 in the tumors.

174 s plasminogen to plasmin, codistributes with laminin-5 in MCF-10A matrix, tPA is not present in pp126

175 role in hemidesmosome assembly by binding to laminin-5 in the basement membrane zone of epithelial ti

176 In addition, plasminogen and tPA bind laminin-5 in vitro.

177 inin-332 (alpha3beta3gamma2; formerly called laminin-5) in some cysts, as also observed in human PKD.

178 nd spreading via integrin alpha(3)beta(1) on laminin 5 independent of RhoGTPase, a regulator of actin

179 isplay parallel changes in the expression of laminin 5, integrin alpha3beta1, E-cadherin, and the gap

180 al or inhibition of any of these components (laminin-5, integrin, CD151, FAK) markedly sensitizes cel

181 Thus, perturbation of the NC1-laminin 5 interaction may contribute to the pathogenesis

182 In contrast, the NC1-laminin 5 interaction was not affected by a monoclonal a

183 ate steps in the pathway linking alpha3beta1-laminin 5 interactions to GJIC indicated that protein tr

184 migratory keratinocytes wherein alpha3beta1-laminin-5 interactions regulate src kinase signaling thr

185 adhesion-blocking mAb to the alpha3 chain of laminin 5 interrupted T cell development.

186 become activated, deposit newly synthesized laminin-5 into the extracellular matrix, and migrate int

187 Laminin 5 is a pivotal hemidesmosomal protein involved i

188 Since laminin 5 is lost in prostate carcinoma, the mechanism o

189 In summary, we provide evidence that laminin-5 is a multifunctional protein that can act unde

190 6F12 (laminin beta 3 subunit) revealed that laminin-5 is deposited in a basal matrix that extends in

191 interaction between integrin alpha3beta1 and laminin-5 is essential for establishment of a stable, le

192 We report here that laminin-5 is found in bone and expressed by hMSC.

193 Laminin-5 is markedly down-regulated in breast cancer ce

194 a4Q155L fail to associate with Shc even when laminin-5 is present, thus impacting downstream signalin

195 We conclude that contact with laminin-5 is sufficient to activate ERK and to stimulate

196 Laminin-5 is the major extracellular matrix component pr

197 labeling indicate that laminin-6, as well as laminin-5, is identified by the AAb from a subset of cic

198 substantially greater than its affinity for laminin-5 (Kd > 600 nM).

199 collagen gene (COL7A1) or the genes encoding laminin 5 (LAMA3, LAMB3, or LAMC2) usually result in cli

200 mutations found primarily in the b3 chain of laminin 5 (LAMB3).

201 amounts of the extracellular matrix protein laminin 5 (LM-5) and frequently express alpha6beta4 inte

202 tate gland, basal epithelial cells adhere to laminin 5 (LM5) via alpha3beta1 and alpha6beta4 integrin

203 d significant increases in the expression of laminin 5 (Ln-5, gamma2 chain) and matrix metalloprotein

204 he recombinant LG3 (rLG3) module (26 kDa) of laminin-5 (Ln-5) alpha(3) chain replicated key Ln-5 acti

205 art to metalloproteinase-induced cleavage of laminin-5 (Ln-5) and enhanced motility of the cancer cel

206 Specific cleavage of laminin-5 (Ln-5) by matrix metalloprotease-2 (MMP2) was

207 Laminin-5 (Ln-5) is an extracellular matrix substrate fo

208 The extracellular matrix macromolecule laminin-5 (Ln-5) is converted by matrix metalloproteinas

209 that matrix metalloprotease-2 (MMP2) cleaves laminin-5 (Ln-5), a basement membrane component, generat

210 membrane (BM) extracellular matrix component laminin-5 (Ln-5), integrins alpha 3 beta 1 and alpha 6 b

211 adhesion to the extracellular matrix protein laminin-5 (Ln-5).

212 eratinocytes on the gamma2 precursor form of laminin 5 (LN5') immediately induced directional hypermo

213 sent evidence that HPV binds specifically to laminin 5 (LN5), a component of the extracellular matrix

214 A (p16) and of the basement membrane protein laminin 5 (LN5).

215 Laminin-5 (LN5) anchors epithelial cells to the underlyi

216 Laminin-5 (LN5) is a matrix component of epithelial tiss

217 and motility of these cells are mediated by laminin-5 ((LN5) and fibronectin (FN) through alpha3beta

218 ction between double negative thymoctyes and laminin 5 made by subcapsular epithelial cells is requir

219 ransfected endothelial cells are plated onto laminin-5 matrix, GFP-beta4WT and GFP-beta4V284E localiz

220 hypothesize that these laminins, along with laminin 5, may play roles in photoreceptor production, s

221 The basement membrane protein laminin-332 (laminin-5) mediates both stable cell adhesion and rapid

222 y, the induction of various genes (including laminin-5, MMPs, TGF beta, zyxin, terminal deoxynucleoti

223 The laminin 5 molecule (previously termed kalinin/nicein/epi

224 embrane components are lacking in the mature laminin 5 molecule.

225 inhibitor of vesicle transport, or with anti-laminin 5 monoclonal antibodies abolishes the PI3K-depen

226 In sequencing the beta3 chain of laminin 5 mRNA from LNCaP cells, we observed three diffe

227 In the non-Herlitz group (laminin 5 mutations, n = 3) the counts were 66.7% +/- 7.

228 After plasmin treatment, pp126 cell laminin-5 not only impedes cell motility but also become

229 Laminin-5, on the other hand, neither polymerized nor co

230 We report that deposition of laminin 5 onto collagen switches adhesion and signaling

231 We suggest that deposition of laminin 5 onto the collagen substratum, as in wound repa

232 ted NC1, as well as a polyclonal antibody to laminin 5 or a monoclonal antibody to the beta3 chain.

233 ha3 or when alpha3beta1 is mainly engaged on laminin 5 or E-cadherin in adherens junctions, leading t

234 Deposition of laminin 5 over exposed dermal collagen in epidermal woun

235 6 and beta4 integrins and the beta3 chain of laminin 5; p < 0.01 for BPAG1; and p < 0.05 for BPAG2.

236 tigated cause-and-effect relationships among laminin 5, p16, hypermotility, and growth arrest.

237 esting that cell-matrix adhesion provided by laminin 5 plays a role in cell survival in vivo.

238 emaining stroma showed negative staining for laminin 5, positive linear staining for collagen IV in t

239 o identify the enzyme(s) responsible for the laminin 5 processing and the sites of proteolytic cleava

240 To characterize the nature of laminin 5 processing, we determined the N-terminal amino

241 This study investigated the mechanism of laminin-5 processing by keratinocytes.

242 a substrate for mTLD, suggesting a role for laminin-5 processing by mTLD in the skin.

243 BMP-1 null mice were shown to have deficient laminin-5 processing.

244 pression of p300 leads to down-regulation of laminin-5 production in breast epithelial cells, resulti

245 t hypoxic extracellular matrix contains less laminin-5 protein than normoxic matrix.

246 B2+ breast cancer cells to basement membrane laminin-5 provides substantial resistance to trastuzumab

247 The alpha6beta4 integrin-a laminin-5 receptor-mediates assembly of hemidesmosomes a

248 ell surface complexes with both of the major laminin-5 receptors, integrins alpha3beta1 and alpha6bet

249 extracellular matrix and not due to loss of laminin-5 receptors.

250 Laminin 5 regulates anchorage and motility of epithelial

251 In contrast, adhesion and spreading on laminin 5 requires integrins alpha(3)beta(1) and alpha(6

252 ssociated tetraspanin protein CD151 reversed laminin-5 resistance and sensitized ErbB2+ cells to tras

253 oactive pro-angiogenic gamma2 fragments from laminin-5, revealing a functional role for cathepsin S i

254 mbly, but not hemidesmosome assembly, in the laminin 5-rich dermal-epidermal junction basement membra

255 Treatment of pp126 laminin-5-rich extracellular matrix with exogenous tPA r

256 In contrast, cells plated onto the laminin-5-rich matrices of pp126 epithelial cells fail t

257 enhanced MMP-dependent cellular migration on laminin-5-rich matrix using an in vitro colony dispersio

258 sed dermal collagen and fibronectin and over laminin 5 secreted into the provisional basement membran

259 we have compared the forms of heterotrimeric laminin-5 secreted by 804G and MCF-10A cells with those

260 The alpha3 subunit of laminin-5 secreted by pp126 cells migrates at 190 kD, wh

261 cules containing one (laminins-6-9) or more (laminin-5) short arm truncations.

262 There was undetectable laminin 5 staining at the dermal-epidermal junction usin

263 On laminin-5 substrates, the epithelial cells rapidly sprea

264 nclude LAMA3, LAMB3, and LAMC2, which encode laminin 5 subunit polypeptides, the alpha3-, beta3-, and

265 se secreted by pp126 cells, using a panel of laminin-5 subunit-specific antibodies.

266 min may play an important role in processing laminin-5 subunits is supported by immunofluorescence an

267 The basement membrane protein laminin-5 supports tumor cell adhesion and motility and

268 ur of these chains, along with components of laminin 5 (the alpha3, beta3, and gamma2 chains) are als

269 n of collagenase and decreased expression of laminin-5, the locomotion brake for keratinocytes.

270 Laminin-5, the major extracellular matrix protein produc

271 synthesize laminin-5 and adhere to exogenous laminin-5 through alpha3beta1 integrin.

272 s from alpha3beta1-deficient skin adhered to laminin-5 through alpha6 integrins.

273 activity was suppressed in cells attached to laminin-5 through the alpha3 integrin receptor.

274 nt anchorage on endogenous basement membrane laminin 5 to migration on exposed dermal collagen.

275 fied type VII collagen bound to fibronectin, laminin-5, type I collagen, and type IV collagen and als

276 ified minicollagen VII bound to fibronectin, laminin-5, type I collagen, and type IV collagen.

277 Expression of BM components, including laminin-5, type IV collagen, type VII collagen, perlecan

278 e wound edge on endogenous basement membrane laminin 5 via alpha(3)beta(1) and alpha(6)beta(4) in a R

279 Spreading on endogenous laminin 5 via alpha(3)beta(1) is necessary but not suffi

280 Adhesion of quiescent HFKs to laminin 5 via integrin alpha(3)beta(1) and alpha(6)beta(

281 The binding of 125I-NC1 to laminin 5 was inhibited by a 50-fold excess of unlabeled

282 the interaction of integrin alpha3beta1 with laminin 5 was sufficient to promote GJIC.

283 the presence of antibodies directed against laminin-5 was also measured.

284 ection of circulating autoantibodies against laminin-5 was developed.

285 ocused our studies on the laminin family, as laminin-5 was identified as an autoantigen in cicatricia

286 mal keratinocyte medium even though abundant laminin-5 was present.

287 t in keratinocytes, haptotactic migration on laminin-5 was stimulated by anti-beta1 integrin-activati

288 PAK1 activation induced by laminin-5 was suppressed by expression of a dominant-neg

289 3 mRNA, encoding the third or beta3 chain of laminin-5, was not markedly reduced.

290 the role of CD151 in tumor cell responses to laminin-5, we used retroviral RNA interference to effici

291 (laminin-6) intensely and a 400-kDa protein (laminin-5) weakly in normal keratinocyte medium even tho

292 n of the alpha3, beta3, and gamma2 chains of laminin 5 were investigated in normal and invasive prost

293 - and alpha6beta4-dependent cell adhesion to laminin-5 were also impaired in CD151-silenced cells.

294 keratinocytes (containing laminin-6 but not laminin-5) were studied by western blotting.

295 binding to laminin 332 (LN332; also known as laminin 5), whereas antibodies that block alpha6beta4 bi

296 GFP-beta4WT and GFP-beta4V284E localize with laminin-5, whereas GFP-beta4K150A and GFP-beta4Q155L do

297 in in the cytoplasm in beta4 mutant cells on laminin-5, whereas they enter effectively into the nucle

298 is a cell adhesion receptor for laminin-332/laminin-5 with important roles in the survival and motil

299 fibulin-2 functions to bridge laminin-1 and laminin-5 with other extracellular matrix proteins, prov

300 is promoted by integrin-mediated adhesion to laminin-5, with strong support by CD151, leading to sign