ライフサイエンスコーパス: leguminosarum

1 l-(beta1-->4)-glucuronosyl transferase in R. leguminosarum.

2 rom the homologous cpn60-1 gene of Rhizobium leguminosarum.

3 s been cloned and characterized in Rhizobium leguminosarum.

4 oth membranes and a cytosolic factor from R. leguminosarum.

5 id A precursor common to both E. coli and R. leguminosarum.

6 with a partially sequenced gene (orf*) of R. leguminosarum.

7 itrogen-fixing pea root nodules by Rhizobium leguminosarum.

8 inducible acyl carrier protein (NodF) of R. leguminosarum.

9 responses and the symbiotic efficiency of R. leguminosarum.

10 as brassicacearum and rhizospheric Rhizobium leguminosarum.

11 re made by the symbiotic bacterium Rhizobium leguminosarum.

12 from Burkholderia caryophylli and Rhizobium leguminosarum.

13 C, (15)N-labeled NodF protein from Rhizobium leguminosarum.

14 ng signalling molecule produced by Rhizobium leguminosarum.

15 d the absence of laurate and myristate in R. leguminosarum.

16 r the nodulation-defective mutant 24AR of R. leguminosarum.

17 A Rhizobium leguminosarum 3841 acpXL mutant (named here Rlv22) lacki

18 phosphate-GalA as a minor novel lipid of R. leguminosarum 3841 and S. meliloti.

19 hybrid cosmid (pMJK-1) containing a 25-kb R. leguminosarum 3841 DNA insert that directs the overexpre

20 1800 lysates of individual colonies of a R. leguminosarum 3841 genomic DNA library in the host strai

21 4000 lysates of individual colonies of an R. leguminosarum 3841 genomic DNA library in the host strai

22 the E. coli system when total lipids from R. leguminosarum 3841 or S. meliloti 1021 were added.

23 de (OPS) isolated from free living Rhizobium leguminosarum 3841, a symbiont of Pisum sativum, using c

24 gions of the lipopolysaccharide in Rhizobium leguminosarum, a nitrogen-fixing plant endosymbiont, are

25 Lipid A of Rhizobium leguminosarum, a nitrogen-fixing plant endosymbiont, dis

26 uch dual-host strains were tested: Rhizobium leguminosarum A34 in peas and beans and Bradyrhizobium s

27 n overproduction in vitro of the expected R. leguminosarum acyltransferase, which is C28-AcpXL-depend

28 ning precursor is present in membranes of R. leguminosarum and R. etli but not in S. meliloti or Esch

29 We now demonstrate that membranes of R. leguminosarum and R. etli can convert B to D-1 in a reac

30 previously been identified in extracts of R. leguminosarum and Rhizobium etli but not Sinorhizobium m

31 Rhizobium leguminosarum and Rhizobium etli contain functional lpxF

32 The structures of Rhizobium leguminosarum and Rhizobium etli lipid A are distinct fr

33 ase in transcriptional activation in both R. leguminosarum and S. meliloti.

34 embrane-bound phosphatase found in Rhizobium leguminosarum and some other Gram-negative bacteria, sel

35 chain amino acid permease (Bra) of Rhizobium leguminosarum and the histidine permease (His) of Salmon

36 The lpcC gene of Rhizobium leguminosarum and the lpsB gene of Sinorhizobium melilot

37 e to Zn was associated predominantly with R. leguminosarum and was likely due to the coordination of

38 inhibits the growth of several strains of R. leguminosarum and was previously known as 'small bacteri

39 s, a 12.5 kDa protein was identified from R. leguminosarum as a putative homolog of IHF subunit beta

40 ated exclusively in the outer membrane of R. leguminosarum as judged by sucrose gradient analysis.

41 y acid (VLCFA) is found in the lipid A of R. leguminosarum as well as in the lipid A of the medically

42 We have identified an R. leguminosarum autoinducer that, together with RhiR, is r

43 The pyrE gene of Rhizobium leguminosarum biovar trifolii (Rl) was subcloned and its

44 Rhizobium leguminosarum biovar viciae strain 3841 is a motile alph

45 logy NodC in the inner membrane of Rhizobium leguminosarum biovar viciae was analysed using a series

46 The homologous interaction between R. leguminosarum bv viciae and its host, pea, was examined

47 genesis, and common nod genes from Rhizobium leguminosarum bv viciae and Rhizobium meliloti, required

48 roots were even found to be colonized by R. leguminosarum bv viciae expressing S. meliloti nod genes

49 bioreporters has been developed in Rhizobium leguminosarum bv viciae strain 3841, and these detect me

50 oots following inoculation with an Exo(-) R. leguminosarum bv viciae strain that produced S. meliloti

51 infection thread formation in response to R. leguminosarum bv viciae, but only when the bacteria expr

52 d with Bradyrhizobium japonicum or Rhizobium leguminosarum bv viciae, respectively, and their respons

53 duction (ini) in response to signals from R. leguminosarum bv viciae.

54 ype superoxide dismutase (SodA) of Rhizobium leguminosarum bv.

55 an spectra (SCRS) to differentiate Rhizobium leguminosarum bv.

56 The mutation was created in R. leguminosarum bv. phaseoli strain 8002, which forms symb

57 zobium NGR234 and R. meliloti, and Psi of R. leguminosarum bv. phaseoli.

58 Rhizobium leguminosarum bv. trifolii 4S has a lipopolysaccharide O

59 saccharides (CLOSs) from wild-type Rhizobium leguminosarum bv. trifolii on development of white clove

60 we have shown that tfxABCDEFG from Rhizobium leguminosarum bv. trifolii T24 is sufficient to confer T

61 -kb fragment was used to transform Rhizobium leguminosarum bv. trifolii TA-1JH, a strain which normal

62 on of CLOS also enabled a NodC- mutant of R. leguminosarum bv. trifolii to progress further in the in

63 We hypothesize that R. leguminosarum bv. viciae 3841 contains an alternate mech

64 The genome of R. leguminosarum bv. viciae 3841, a pea-nodulating endosymb

65 ronmental cue(s) triggering chemotaxis of R. leguminosarum bv. viciae cells towards the roots of pea

66 hizobium caulinodans may be secreted from R. leguminosarum bv. viciae in a prsD-dependent manner.

67 roduct is highly homologous to the Rhizobium leguminosarum bv. viciae RhiR protein and a number of ot

68 NodO is a secreted protein from Rhizobium leguminosarum bv. viciae with a role in signalling durin

69 In Rhizobium leguminosarum bv. viciae, quorum-sensing is regulated by

70 a Cpn60 protein from the bacterium Rhizobium leguminosarum can function to allow E. coli growth at 37

71 rs modulate the motility swimming bias of R. leguminosarum cells and that the che1 cluster is the maj

72 lipid A isolated by pH 4.5 hydrolysis of R. leguminosarum cells is more heterogeneous than previousl

73 amounts of AHLs synthesized over time by R. leguminosarum cells with and without the symbiosis plasm

74 Membranes of Rhizobium leguminosarum contain a 3-deoxy-D-manno-octulosonic acid

75 eptose (heptose), while the inner core of R. leguminosarum contains 2-keto-3-deoxy-D-manno-octulosoni

76 The endosymbiotic bacterium Rhizobium leguminosarum contains a single hydrogenase system that

77 -negative bacteria, and the inner core of R. leguminosarum contains mannose and galactose in place of

78 oposed to represent a key early enzyme in R. leguminosarum core assembly.

79 The inner portion of the R. leguminosarum core contains mannose, galactose, and thre

80 iated transcriptional activation from the R. leguminosarum dctA promoter both in vivo and in vitro.

81 iated transcriptional activation from the R. leguminosarum dctA promoter.

82 IHF binding sites adjacent to the Rhizobium leguminosarum dctA promoter.

83 In contrast to R. leguminosarum dctA, the Sinorhizobium meliloti dctA prom

84 S. meliloti lpsB complements a mutant of R. leguminosarum defective in lpcC, but the converse does n

85 e of the nitrogen-fixing bacterium Rhizobium leguminosarum differs from that of Escherichia coli in s

86 The lipopolysaccharide of Rhizobium leguminosarum differs from that of other Gram-negative o

87 from the nitrogen-fixing bacterium Rhizobium leguminosarum displays many structural differences compa

88 ch contains at least 20 kilobase pairs of R. leguminosarum DNA.

89 s introduced into the IHF binding site of R. leguminosarum dtA that reduced the affinity of the promo

90 ugh PtdIns is not detected in cultures of R. leguminosarum/etli (CE3), PtdIns may be synthesized duri

91 Aap from R. leguminosarum expressed in E. coli also promoted efflux

92 mbrane-associated glycosyl transferase in R. leguminosarum extracts that incorporates mannose into na

93 e any of the unique reactions detected in R. leguminosarum extracts.

94 annose and/or UDP-galactose, membranes of R. leguminosarum first transferred mannose and then galacto

95 hree novel GalA transferases from a 22-kb R. leguminosarum genomic DNA insert-containing cosmid (pSGA

96 romoter results in the production of each R. leguminosarum glycosyltransferase in E. coli membranes i

97 detoxification in plants inoculated with R. leguminosarum has particular relevance to PGPB enhanced

98 Rhizobium leguminosarum has two high-affinity Mn(2+) transport sys

99 dic exopolysaccharides (EPSs) produced by R. leguminosarum in a calcium-dependent manner, sustaining

100 and other methods that RapA2 from Rhizobium leguminosarum indeed exhibits a cadherin-like beta-sheet

101 Rhizobium leguminosarum is a Gram-negative bacterium that forms ni

102 Rhizobium leguminosarum is a soil bacterium that infects root hair

103 ore of the Gram-negative bacterium Rhizobium leguminosarum is more amenable to enzymatic study than t

104 te that the synthesis of multiple AHLs in R. leguminosarum is regulated by complex mechanisms that op

105 t endosymbionts Rhizobium etli and Rhizobium leguminosarum is the presence of a proximal sugar unit c

106 ght-binding kinetics) of LpxC from Rhizobium leguminosarum (Ki = 340 nM), a Gram-negative plant endos

107 ylated at positions 1 and 4', R. etli and R. leguminosarum lipid A consists of a mixture of structura

108 R. leguminosarum lipid A is esterified with a peculiar long

109 te these differences, the biosynthesis of R. leguminosarum lipid A is initiated by the same seven enz

110 An especially striking feature of R. leguminosarum lipid A is that it lacks both the 1- and 4

111 Rhizobium leguminosarum lipid A lacks both phosphates, but contain

112 R. leguminosarum lipid A lacks phosphate groups, but it con

113 R. leguminosarum lipid A lacks the usual 1- and 4'-phosphat

114 l structural features shared with R. etli/R. leguminosarum lipid A may be essential for symbiosis.

115 R. leguminosarum lipid A often contains an aminogluconic ac

116 Coexpression of FnLpxE and the Rhizobium leguminosarum lipid A oxidase RlLpxQ in E. coli converts

117 ry acyl chains attached to E. coli versus R. leguminosarum lipid A, specifically the presence of 27-h

118 lipid A and inner core regions of Rhizobium leguminosarum lipopolysaccharide contain four galacturon

119 In R. leguminosarum lipopolysaccharide, the inner core is modi

120 he lipid A from Rhizobium etli and Rhizobium leguminosarum lipopolysaccharides (LPSs) lacks phosphate

121 waaC-waaF deletion mutant expressing the R. leguminosarum lpcC gene likewise generates a hybrid LPS

122 the chromosomal lpxC gene is replaced by R. leguminosarum lpxC is resistant to CHIR-090 up to 100 mi

123 R. leguminosarum LpxC therefore provides a useful control f

124 KM (4.8 microM) and the kcat (1.7 s-1) of R. leguminosarum LpxC with UDP-3-O-[(R)-3-hydroxymyristoyl]

125 the purification and characterization of R. leguminosarum LpxE.

126 cation and characterization of the Rhizobium leguminosarum mannosyl transferase LpcC, which adds a ma

127 The GDP-mannose-dependent enzyme of R. leguminosarum may represent a functional equivalent of E

128 li generates the same products as seen in R. leguminosarum membranes.

129 In the N2-fixing bacterium Rhizobium leguminosarum, mutations in a homologue of tonB (tonB(Rl

130 r hydrolase/phosphodiesterase from Rhizobium leguminosarum (R/PMH) both structurally and kinetically.

131 The nitrogen-fixing symbiont Rhizobium leguminosarum reportedly produces at least six different

132 quorum-sensing (QS) regulation in Rhizobium leguminosarum revealed an unusual type of gene regulatio

133 yl chain is present in extracts of Rhizobium leguminosarum, Rhizobium etli, and Sinorhizobium melilot

134 ily Rhizobiaceae, being present in Rhizobium leguminosarum, Rhizobium fredii, Rhizobium meliloti, Rhi

135 or is more widespread, cell extracts from R. leguminosarum, Rhizobium sp. strain NGR234, Rhizobium fr

136 EI(Ntr) of the PTS(Ntr) system in Rhizobium leguminosarum strain Rlv3841 caused a pleiotropic phenot

137 To this end, Rhizobium leguminosarum strains nodulating sympatric species of na

138 sment of the role of the 1-phosphatase in R. leguminosarum symbiosis with plants.

139 describe a membrane-bound deacylase from R. leguminosarum that removes a single ester-linked beta-hy

140 ne enzyme and a cytosolic acyl donor from R. leguminosarum, that transfers 27-hydroxyoctacosanoic aci

141 In contrast to Rhizobium etli and Rhizobium leguminosarum, the NGR234 lipid A contains a bisphosphor

142 the rarely used heterologous host Rhizobium leguminosarum to invoke the activities of two cobalamin-

143 s of heptose in E. coli and of mannose in R. leguminosarum to Kdo are both alpha1-5.

144 Purified CinS bound to the R. leguminosarum transcriptional regulator PraR, which repr

145 ic acyl donor was purified from wild-type R. leguminosarum using the acylation of (Kdo)2-[4'-32P]-lip

146 In this paper, a mutant of R. leguminosarum was created by placing a kanamycin resista

147 The double sitA mntH mutant of R. leguminosarum was unable to fix nitrogen (Fix(-) ) with

148 ibution of chemotaxis to the lifestyle of R. leguminosarum, we have characterized the function of two

149 strain S88 and the pssDE genes of Rhizobium leguminosarum were identified as encoding glucuronosyl-(