ライフサイエンスコーパス: lethal mutation

1 t position 102 or 104 of RKLKR resulted in a lethal mutation.

2 ced in cis with the plasmid-encoded dominant lethal mutation.

3 led that Mom2R encodes a recessive embryonic lethal mutation.

4 the viral gene encoding either gM or gN is a lethal mutation.

5 used to molecularly map previously isolated lethal mutations.

6 for studying the maternal effects of zygotic lethal mutations.

7 nd numbers of genes carry similar numbers of lethal mutations.

8 2F alleles among a collection of EMS-induced lethal mutations.

9 ntralized phenotype similar to dpp embryonic lethal mutations.

10 is shown to complement yeast GUK1 recessive lethal mutations.

11 families and obtained 9 recessive embryonic lethal mutations.

12 screens been conducted to isolate embryonic lethal mutations.

13 orates beneficial, neutral, deleterious, and lethal mutations.

14 e's essential genes by temperature-sensitive lethal mutations.

15 ing the maternal effect of recessive zygotic lethal mutations.

16 an adaptive mutant strain are threatened by lethal mutations.

17 for circumventing the effects of potentially lethal mutations.

18 GC transitions, which can eventually lead to lethal mutations.

19 ) by recombinational rescue of conditionally lethal mutations.

20 ific chromosomal translocations can generate lethal mutations.

21 for example synthetic phage genomes carrying lethal mutations.

22 ially leading to the stable incorporation of lethal mutations.

23 intain and analyze stocks carrying recessive lethal mutations.

24 rtion mutations, enabling us to recover 2500 lethal mutations.

25 mate of assumptions, such as the fraction of lethal mutations.

26 Moreover, none are intact; all have lethal mutations [1, 3, 4].

27 We have screened for zygotic embryonic lethal mutations affecting cuticular morphology in Nason

28 to the isolation of two classes of recessive lethal mutations affecting early dorsoventral pattern fo

29 f G --> A excess and predicted indicators of lethal mutation and applied this model to 325 920 unique

30 between accessible functional groups of the lethal mutation and those of the viable mutation and wil

31 anced sensitivity to a temperature-sensitive lethal mutation and to the neurodegenerative effects pro

32 enous S. pombe gamma-tubulin, resulted in 11 lethal mutations and 12 cold-sensitive mutations.

33 fluorescent protein efficiently rescues cac lethal mutations and allows in vivo analysis of calcium

34 t greatly enhances the rate of appearance of lethal mutations and dominant female sterility.

35 initiated with the isolation and mapping of lethal mutations and genome rearrangements in the region

36 With screens for orc2-1 synthetic lethal mutations and Orc2p two-hybrid interactors, a nov

37 n against hepatitis C virus (HCV) by causing lethal mutations and suppressing RNA polymerase in vitro

38 ed to analyse the phenotype of the recessive-lethal mutations and used to show that they blocked cell

39 the presence of nearby proline residues and lethal mutations, and the presence of nearby alanines re

40 osin II heavy chain, and cells carrying this lethal mutation are defective in actomyosin ring assembl

41 Here we describe a recessive lethal mutation, arkadia, generated using gene-trap muta

42 We present a strategy that allows mapping of lethal mutations, as well as viable mutations with visib

43 In a screen for zygotic lethal mutations associated with maternal effects, we ha

44 S7 is a dominant temperature-sensitive (DTS) lethal mutation at 29 degrees that also acts as a recess

45 proximity, a mild mutation at site 901 and a lethal mutation at site 913.

46 In contrast, lethal mutations at the contact with p15/ARPC5 led to un

47 Lethal mutations at the contact with p19/ARPC4 specifica

48 characterization of a temperature-sensitive lethal mutation, bamAE373K, which alters the fifth polyp

49 r identifying and studying recessive zygotic lethal mutations because unfertilized eggs develop as ma

50 ctures, having a viable (C2658U*G2663A) or a lethal mutation (C2658G*G2663C), were determined at 1.75

51 Therefore, the slight deficit of a non-lethal mutation can be detected and characterized.

52 development, demonstrate that X-linked male lethal mutations can be recovered from ENU mutagenesis s

53 Finally, we characterize an embryonic lethal mutation caused by endogenous splicing disruption

54 Lethal mutations clustered in the amino-terminal half of

55 Classes of mutants include conditional lethal mutations, conditional auxotrophs, and conditiona

56 viability due to the uncovering of recessive lethal mutations correlated with an increase in gross ch

57 R4 use is constrained by relatively frequent lethal mutations, deep fitness valleys, and requirements

58 here on pickwickm171 (pikm171), a recessive lethal mutation discovered in a large-scale genetic scre

59 Previously, a dominant temperature-sensitive lethal mutation, dnaT1, had been isolated in E. coli 15T

60 N-induced cytidine deaminase that introduces lethal mutations during retroviral reverse transcription

61 Three new recessive lethal mutations, E170A, D194A, and R206A, were identifi

62 e yielded five recessive csl (cep1 synthetic lethal) mutations, each defining a unique complementatio

63 Two new recessive lethal mutations, F256A and Y330A, were identified.

64 rosophila through screens of maternal effect lethal mutations for defects in spindle pole behaviour.

65 lethal phenotype and separated the recessive lethal mutation from the P-element by recombination.

66 Larval lethal mutations generated by ENU mutagenesis affect dif

67 thers are homozygous for the maternal effect lethal mutation gnu (GNU embryos) under DNA synthesis bu

68 these, 455 correspond to genes for which no lethal mutation has yet been reported.

69 Exquisite embryonic lethal mutations have been isolated in hundreds of genes

70 in that acted as intragenic suppressors of a lethal mutation (I485N) mapping to the peptide-binding d

71 ntation analysis of P23 RNA that contained a lethal mutation in 2C confirmed these results.

72 In addition, a mutant with a conditional lethal mutation in lpxC, an essential gene required for

73 udy as to how fish tolerate what is an early lethal mutation in mammals could facilitate improvement

74 That the PE deletion is a lethal mutation in normative environments suggests that

75 As predicted by the genetic results, a lethal mutation in region I resulted in loss of Est3p fr

76 In BirA, G154D was a lethal mutation in single copy, and the purified protein

77 naturally occurring, recessive, perinatally lethal mutation in the aggrecan core protein gene, cmd(b

78 From a genetic mosaic screen, we find that a lethal mutation in the Drosophila Down syndrome cell adh

79 r growth mutant orb2-34, which carries a non-lethal mutation in the essential gene shk1(+)/pak1(+)/or

80 ppressors of LG4, a virus with a conditional lethal mutation in the gene encoding ICP27.

81 distant intragenic suppressor of a dominant lethal mutation in the guanine nucleotide-binding region

82 omosome because it can be used to maintain a lethal mutation in the inversion interval as a self-sust

83 f-function, temperature-sensitive, embryonic lethal mutation in the maternally required gene gad-1 (g

84 Analysis of recombinant viruses carrying a lethal mutation in the NES of ICP27 was not accomplished

85 Somatic homozygous clones of an embryonic lethal mutation in this gene (stru1A122) cause wing blis

86 A lethal mutation in trol results in the failure of quiesc

87 We recently characterized a lethal mutation in ytr, a conserved gene that encodes a

88 Not really finished (nrf), a larval-lethal mutation in zebrafish generated by retroviral ins

89 onal alleles of foxi one (foo), an embryonic lethal mutation in zebrafish that displays defects in bo

90 An analysis of lethal mutations in a PS integrin gene showed that the i

91 Two conditionally lethal mutations in bimD arrest with aberrant mitotic sp

92 In screening for embryonic-lethal mutations in Caenorhabditis elegans, we defined a

93 dosage suppressor analysis of 53 conditional lethal mutations in cell division cycle and RNA synthesi

94 ic transmission, we have isolated homozygous lethal mutations in Drosophila importin 13 (imp13).

95 Propagation of viruses bearing those lethal mutations in G completely depended on complementa

96 authentic recombinant VSV particles bearing lethal mutations in G, confirms that the hydrophobic fus

97 Recently, we reported a genetic screen for lethal mutations in gene 2.5 that we are using to identi

98 A screen for lethal mutations in gene 2.5 uncovered a variety of esse

99 complementation assay was used to screen for lethal mutations in gene 2.5.

100 sh have led to the isolation of thousands of lethal mutations in genes that are essential for embryon

101 mutagenesis was used to generate conditional-lethal mutations in hitherto-uncharacterized domains of

102 within conserved motifs and demonstrate that lethal mutations in predicted ATP binding-hydrolysis mot

103 ly and identified second-site suppressors of lethal mutations in SP.

104 ned the phenotypes of strong and weak larval lethal mutations in spaghetti squash (sqh), which encode

105 h to identify 11 DHR3 mutants from a pool of lethal mutations in the 46F region on the second chromos

106 Moreover, phenotypically lethal mutations in the carboxyl-terminal WD-40 repeats

107 rformed genetic screens to isolate recessive lethal mutations in the chromosomal region that includes

108 ribe new myo2 alleles containing conditional lethal mutations in the COOH-terminal tail domain.

109 sites in the genome, allowing the rescue of lethal mutations in the corresponding genes.

110 n's structure and function, we identified 13 lethal mutations in the Drosophila kinesin heavy chain m

111 have used previously characterized recessive lethal mutations in the dynein heavy chain gene, Dhc64C,

112 Lethal mutations in the extended arm drastically reduced

113 uthentic recombinant viral particles bearing lethal mutations in the G gene.

114 We show here that maternal-effect lethal mutations in the gene mex-3 cause descendants of

115 A binding, we isolated an assortment of heat-lethal mutations in the genes encoding RPA2 and RPA3.

116 tions of HSF in Drosophila, we have isolated lethal mutations in the hsf gene.

117 Lethal mutations in the region are suppressed by Cus1-54

118 carried out a large-scale genetic screen for lethal mutations in the region.

119 We have studied lethal mutations in the single calmodulin gene (Cam) of

120 the utility of the method by applying it to lethal mutations in the synaptic transmission genes syna

121 large-scale mutagenesis screen for embryonic lethal mutations in zebrafish Danio rerio we have found

122 genetic screen for zygotic effect, embryonic lethal mutations in zebrafish we have identified 25 muta

123 Whereas mutations in Dm-myb are lethal, mutations in mip130 are viable.

124 Lethal mutations, in particular those that perturbed par

125 expressed in mast cells, including embryonic lethal mutations, in vitro or in vivo.

126 tion groups were defined by seven prenatally lethal mutations, including a group (l7R3) comprised of

127 we report that two independent, postnatally lethal mutations induced by N-ethyl-N-nitrosourea and ma

128 Conditional lethal mutations inserted into mosquito genomes allow fo

129 nt bacterial strains do not evolve because a lethal mutation is required to gain immunity.

130 Surprisingly, five lethal mutations lie outside all polymerase homology in

131 Cx26-G45E is a lethal mutation linked to KIDS that forms constitutively

132 oids, the presence of PRR alone may confer a lethal mutation load or, alternatively, PRR alone may be

133 mutations may be useful for balancing other lethal mutations located in the distal region of LG 12.

134 We describe a lethal mutation mouse strain generated using promoter-tr

135 We have isolated a new conditional-lethal mutation, ndc10-2, in the NDC10/CBF2/CTF14 gene t

136 mutagenesis of lambda's A gene and found ten lethal mutations, nine of which cause post-cleavage pack

137 sociated with nucleotide binding sites where lethal mutations occur.

138 A non-lethal mutation of CFD1 (cfd1-1) reduced c-aconitase spe

139 d DNA strand-exchange and rescuing the ssb-1 lethal mutation of E. coli respectively.

140 A recently isolated, lethal mutation of the homeotic Abdominal gene of the re

141 The NtPSA1 cDNA was used to complement a lethal mutation of the yeast PRC1 alpha subunit gene ind

142 Biochemical analysis showed that lethal mutations of Asp425, Arg463, Arg511 and His515 in

143 Screening for suppressors of dominant lethal mutations of essential genes is challenging as th

144 ted the ability of wild-type SCNM1 to rescue lethal mutations of I-mfa and Brunol4.

145 Genetic analyses reveal that lethal mutations of MAGUKs often occur in the guanylate

146 actor-independent peptide synthesis; nor did lethal mutations of nucleotides that abolish the binding

147 Recessive lethal mutations of the lethal(2)giant discs (l(2)gd) an

148 e use temperature-sensitive (ts) conditional lethal mutations of the S. cerevisiae POL2 and POL3 gene

149 diated by cytidine deamination, which causes lethal mutations of the viral genome.

150 regulate cell movement in vivo, we screened lethal mutations on chromosome 3R for defects in border

151 We conducted a mosaic genetic screen of lethal mutations on the Drosophila X chromosome to ident

152 e lines are segregating or are now fixed for lethal mutations on the mutagenized chromosome.

153 nk patterning and morphogenesis; we screened lethal mutations on the second chromosome for those that

154 infection in at least two ways: by inducing lethal mutations on the viral cDNA; and by blocking step

155 However, when we subsequently mapped the lethal mutations onto a model of the structure of the mu

156 se of an increased probability of generating lethal mutations or inducing apoptosis.

157 most resistant replicon was able to rescue a lethal mutation (P540A) in NS5B that disrupts its intera

158 s type of insertional mutagen is 1 embryonic lethal mutation per 70-100 proviral insertions, screenin

159 associate with each other, while one of the lethal mutations qkI(kt4) with a single amino acid chang

160 e viability may not be much greater than the lethal mutation rate (0.01 in these lines), but the resu

161 ent-dependent dominant lethal, and recessive lethal mutation rates.

162 s the inbreeding load of both lethal and non-lethal mutations, reducing the amount of inbreeding depr

163 Finally, heterozygous DmORC2 recessive lethal mutations resulted in a suppression of PEV.

164 Zebrafish embryos with the recessive lethal mutations santa (san) and valentine (vtn) do not

165 We have isolated an embryonic lethal mutation, SBU2, that causes somite formation defe

166 L63-specific lethal mutations showed that L63 is required not only fo

167 d in these pairs show decreased frequency of lethal mutations, suggesting their specific role in male

168 alyronz12 (aln) is a recessive lethal mutation that affects early stages of neural cres

169 is plant carries a semidominant, conditional lethal mutation that confers constitutive expression of

170 amn) mouse, which has a recessive, embryonic lethal mutation that interferes specifically with the fo

171 In addition, recessive-lethal mutations that affect residues highly conserved o

172 een of >4000 mutagenized chromosomes bearing lethal mutations that affected multiple aspects of larva

173 Early screens for conditional lethal mutations that affected rRNA expression in Escher

174 Screens for zygotic lethal mutations that are associated with specific mater

175 o tolerate mutations rather than having more lethal mutations that are not observed.

176 tisubunit IN-viral DNA complex, we found the lethal mutations that caused virus morphogenesis defects

177 In previous work with phage lambda, lethal mutations that changed ATP-reactive residues 46 a

178 y chain gene was used to isolate EMS-induced lethal mutations that define at least eight essential ge

179 1 that uncouple metamorphosis, and embryonic-lethal mutations that map to common sequences encoding t

180 of visceral leishmaniasis, are conditionally lethal mutations that render the insect vector form of t

181 gene in a genetic screen to recover X-linked lethal mutations that require this transgene for viabili

182 However, many of these are perinatal lethal mutations that result in death from respiratory d

183 on in A. nidulans, we searched for synthetic lethal mutations that significantly reduced growth in th

184 ous mutagenesis studies identified recessive lethal mutations that were rescued by a genomic fragment

185 applicable strategy for transferring zygotic lethal mutations through the zebrafish germ line.

186 e an obligate diploid that carries recessive lethal mutations throughout the genome.

187 that influence the effect of early embryonic lethal mutations, thus furthering knowledge of genetic i

188 domain are severely affected by the class II lethal mutations; thus, the mutant sequences should be v

189 viously mapped these X-linked dominant, male-lethal mutations to an overlapping region of 600 kb that

190 Embryonic lethal mutations (total of 34) were overwhelmingly the l

191 L5Jcs1 is a perinatal lethal mutation uncovered in a screen for ENU-induced mu

192 Further, cells with proviruses containing lethal mutations upstream of CTL epitopes can also be re

193 A recessive embryonic lethal mutation was obtained.

194 A screen for synthetic lethal mutations was carried out with an rtf1 deletion m

195 other vector that is suitable for generating lethal mutations was constructed in a plasmid containing

196 ells that carry a temperature-sensitive cell-lethal mutation, we conditionally ablate patches of tiss

197 genetic approach to isolate lin-35 synthetic-lethal mutations, we have identified redundant roles for

198 ctions, a series of absolute and conditional lethal mutations were generated.

199 A total of 79 lethal mutations were isolated, representing at least 17

200 sociated with the lethal spotted and piebald lethal mutations which manifest only in the large intest

201 is similar to the lethal spotted and piebald lethal mutations, which are due to defects in endothelin

202 Maternal-effect lethal mutations with a partitioning defective phenotype

203 dentifying Mu insertion sites linked to seed-lethal mutations with a preliminary success rate of near

204 Therefore, an efficient approach to study lethal mutations would be useful.