ライフサイエンスコーパス: leucine residue

1 mino acid at position 65 (either a serine or leucine residue).

2 s -2 to +6 relative to the primary conserved leucine residue.

3 enzymes at a completely conserved C-terminal leucine residue.

4 RNA(Ser) amber suppressor from a serine to a leucine residue.

5 in SOAR1, which in SOAR2 is substituted by a leucine residue.

6 superantigens should focus on this critical leucine residue.

7 though this sequence preserved only a single leucine residue.

8 t the tryptophan pocket is now occupied by a leucine residue.

9 ivates P-factor by removal of the C-terminal leucine residue.

10 a methionine residue in place of the native leucine residue.

11 butions from conserved aromatic, acidic, and leucine residues.

12 he heptad leucine repeat region, rather than leucine residues.

13 and atypical processing sites C-terminal to leucine residues.

14 the LamB hydrophobic core were converted to leucine residues.

15 x was replaced with isologous asparagine and leucine residues.

16 rs retained the hydrophobic character of the leucine residues.

17 on of the entire sequence through the lysine-leucine residues.

18 lly contain an essential tyrosine or pair of leucine residues.

19 Mutation of leucine residues 1010 and 1011 motif in the EGF receptor

20 d X-ray structures indicate that a conserved leucine, residue 103 in the Themiste zostericola Mhr seq

21 Mutation of leucine residue 238 of this NES motif abolished the inte

22 Leucine residues 357, 358, and 361 of nCLU were necessar

23 monomeric mutant of phospholamban, in which leucine residue 37 was changed to alanine.

24 All four leucine residues (a position) and three valine residues

25 imerization and that mutation of a conserved leucine residue abolishes self-association.

26 in the meprin alphaT domain GXXXG motif with leucine residues, alanine insertions in the meprin alpha

27 ed to substitute a glutamine for a conserved leucine residue and may hinder processing of SP-C precur

28 onversion of a conserved proline (1173) to a leucine residue and the other a deletion of an isoleucin

29 ent NF-kappaB activation are mediated by two leucine residues and a tryptophan-containing motif in th

30 he deduced PICBP protein sequence is rich in leucine residues and contains three classes of repeats.

31 In addition, the two leucine residues and four of the five negatively charged

32 e initially replaced the core region with 12 leucine residues and then constructed recovery-of-functi

33 mally altered tRNA(Ser) exclusively inserted leucine residues and was an excellent in vitro substrate

34 n not as the canonical methionine but as the leucine residue, and its activity was independent of ups

35 One face displays hydrophobic leucine residues, and the opposite face displays hydroph

36 of off-rotamer conformations are largest for leucine residues (approximately 6 % versus 16.6 %).

37 sults suggest that helix 4 and the conserved leucine residue are part of a pRBR-binding interface in

38 not observed in other mutants and all other leucine residues are ordered in this structure, it is li

39 naturally occurring HBV variant containing a leucine residue at amino acid 97 of the core protein was

40 Although HSV-1 and HSV-2 UL52s contain a leucine residue at position 986, most other herpesvirus

41 e residues (605, 617, and 620), and that the leucine residue at the +2 position from the pTyr interac

42 Importantly, the leucine residue at this position is highly conserved amo

43 rowth factor receptor (EGFR) contains two di-leucine residues at 658 (TLRRLLQER) and 679 (NQALLRIL).

44 acing valine residues at each a position and leucine residues at each d position of the heptad repeat

45 Whereas the introduction of CalphaMe-leucine residues at positions 27 and either 18 (11), 37

46 Mamu-A*01 because they contained proline and leucine residues at positions 3 and 9, respectively.

47 were mutated singly to alanines, and the two leucine residues at positions 539 and 548 were mutated d

48 ivity, or ligand-induced degradation, the di-leucine residues at positions 658 or 679 were mutated to

49 the sequence isomer in which the lysine and leucine residues at positions R6 and R7 are swapped has

50 agonists, we introduced two or more CalphaMe-leucine residues at positions shown in earlier studies t

51 three "classical" heptad repeat motifs with leucine residues at the "d position," and a distinctive

52 quence repeat, abcdefg , with isoleucine and leucine residues at the a and d sites to ensure dimeriza

53 modified these trimer designs by introducing leucine residues at V3 positions 306 and 308 to create h

54 MARTX toxin at other sites, specifically at leucine residues between the effector domains.

55 The major recognition events are the two leucine residues binding in hydrophobic pockets on sigma

56 Substitution of leucine residues by 5,5,5-trifluoroleucine at the d-posi

57 the distal histidine has been replaced by a leucine residue, CcP(H52L), has been investigated as a f

58 entire syndecan-1 transmembrane domain with leucine residues completely blocks the cell spreading.

59 Glycine substitution for selective leucine residues confirmed that MYOC-MYOC interactions o

60 associating protein with a high frequency of leucine residues (Daple) interacts with PCP and cell-int

61 s leptomycin B-sensitive and mutation of the leucine residues decreased or eliminated nuclear export

62 that involved either of the two end heptadic leucine residues did not significantly affect fusion.

63 pairs of closely spaced phenylalanines and a leucine residue distributed throughout the N-terminal 10

64 Nonetheless, mutations of a quartet of leucine residues (either single or multiple mutants) in

65 digitating hexa- and penta-heptad repeats of leucine residues flanked by a very basic region.

66 yl thiol pro-nucleophile, while the two tert-leucine residues flanking a central thiourea hydrogen-bo

67 cterized by an amino-terminal domain rich in leucine residues followed by a carboxy-terminal acidic t

68 putative bZIP-like domains and corresponding leucine residues for interaction and modulation of the t

69 ine-rich region, we targeted all neighboring leucine residues for mutagenesis.

70 3)H]TDBzcholine incorporated into homologous leucine residues, gammaLeu-109 and deltaLeu-111.

71 ble positions of methyl groups in valine and leucine residues have been linked to molecular motions a

72 ion of a proline residue in position 80 to a leucine residue in a small endomembrane protein encoded

73 hyl group dynamics of a key hydrophobic core leucine residue in chicken villin headpiece subdomain pr

74 Mutation of the leucine residue in helix 4 of CaLCuV AL1 reduced binding

75 S2 revealed the importance of its C-terminal leucine residue in infectious particle production.

76 Substitution of the conserved leucine residue in the BH3 domain of BimEL for alanine (

77 This is the first example of a leucine residue in the hinge loop that blocks the ATP bi

78 are cleaved at Site-1, which is located at a leucine residue in the middle of the hydrophobic loop in

79 A conserved leucine residue in the midpoint of the second transmembr

80 fied P35delL mutation leads to the loss of a leucine residue in the protein.

81 s in the substitution of a tryptophan with a leucine residue in the ubiquitination domain.

82 ubstitutions in place of the tyrosine or the leucine residue in the YxxL motif demonstrated small, as

83 study, we substituted a proposed stabilizing leucine residue in the zipper domain with a helix-disrup

84 al shielding tensors of alanine, valine, and leucine residues in a series of crystalline peptides of

85 ther, our observations suggest that the four leucine residues in Ca(V)beta3 form a hydrophobic pocket

86 Furthermore, double mutants in these leucine residues in each of A3F's two CD domains (A3F L3

87 e replacements at any of three isoleucine or leucine residues in either alpha1, beta2 or beta3 comple

88 By contrast, alanine replacements for leucine residues in either beta4 or beta6 destabilize bu

89 Alterations to one of these specific leucine residues in either of the two A3F CD domains (A3

90 Mutational analysis of key leucine residues in helix alpha1 demonstrated a role for

91 ding frame encodes a 7-kDa protein with five leucine residues in heptad repeats followed by a basic r

92 Additionally, we introduced CalphaMe-leucine residues in lieu of leucine at positions 14, 15,

93 m solution NMR data for alanine, valine, and leucine residues in proteins show major differences, wit

94 In addition, two leucine residues in the C-terminal leucine-rich repeat r

95 ovel allele of ZIP1, zip1-4LA, in which four leucine residues in the central coiled-coil domain have

96 Mutation of the leucine residues in the common motif of MBP-1 also abrog

97 ch mutations by randomizing three contiguous leucine residues in the DNA-binding domain, and then by

98 er ovary cells frequently alter a cluster of leucine residues in the H6-H7 loop region of beta-tubuli

99 ggested to form intermolecular contacts with leucine residues in the hydrophobic groove (L155, L158,

100 Leucine residues in the leucine-rich region of RFX5(N) (

101 in the repressor domains indicated that the leucine residues in the LXVXL motif are required for tra

102 Moreover, we confirm that some leucine residues in the M domain participate in the in-r

103 exception of the substitution of serine and leucine residues in the W3A1 ETF protein for the human r

104 The leucine residues in this motif are required for the down

105 The leucine residues in this motif were required for the int

106 g preference of the protease for proline and leucine residues into a cathepsin L-like preference for

107 the hydrophobic core comprises six layers of leucine residues introduced at the "a" and "d" positions

108 The hydrophobic nature of the leucine residue is a primary determinant of its function

109 A conserved leucine residue is found at the P1 position of all four

110 of V3L, electron density corresponding to a leucine residue is present in a position that stabilizes

111 In the second series, a leucine residue is replaced with five-, six-, and seven-

112 Deuterating the individual leucine residues (isopropyl-d(7)) permits the use of sol

113 Furthermore, mutation of leucine residues (L-->A) within a nuclear exclusion sign

114 rectifier by substitution of a proline for a leucine residue (L147P).

115 ophobic sequence stretches (with the central leucine residues L20 and L66) in the first and second TM

116 system-mediated degradation, among which two leucine residues (L268 and L269) were critical for the p

117 tations reveals that double mutations of the leucine residues (L317D+L335D) may disrupt the interacti

118 sidues 38 to 55 and contains three conserved leucine residues, L38, L45, and L52, conforming to the h

119 r single-site substitutions at the conserved leucine residues (L45 and L52), caused a significant dec

120 utational analyses revealed that a conserved leucine residue (Leu-64) on the third helix that would n

121 ning mutagenesis of this region identified a leucine residue (Leu-678), which is required for hAhR ac

122 A leucine residue, Leu-98, lines the O(2)-binding pocket i

123 analyses, we examined the importance of two leucine residues, Leu-112 and Leu-156, in the ECD of the

124 A leucine residue, Leu278, further stabilizes nonphospho b

125 n the peptide helix, onto which are appended leucine residue-like substituents.

126 Point mutation at three conserved leucine residues located on the predicted dimer interfac

127 r all conditions examined, indicating that a leucine residue may effectively replace tyrosine for int

128 of the monohalogenated phenols, including a leucine residue near the back edge of the heme (L100).

129 ts with beta-branched residues and conserved leucine residues occupying the a and d positions of the

130 this pocket more deeply than the equivalent leucine residue of pKID.

131 that although the cytoplasmic domain and the leucine residue of the "LSXWS" box are not critical for

132 ckground levels by global replacement of the leucine residues of GFPm by 5,5,5-trifluoroleucine.

133 e studies presented here we demonstrate that leucine residues of helix I (alphaI) regulate tyrosine a

134 which Leu 9, Leu 13, and all four intrinsic leucine residues of melittin were replaced by 5,5,5-trif

135 ckets in the MIT domain bind three conserved leucine residues of the CHMP motif, and mutations that i

136 Individual mutation of the two leucine residues of the endocytic dileucine motif in the

137 role of this motif for channel function, the leucine residues of the zipper were individually mutated

138 oducing point mutations in either one or two leucine residues of this NES sequence abolished the nucl

139 by the ligand binding domains, and the three leucine residues on helix 10 of TR2 are critical for thi

140 lass II reporter gene, confirming that these leucine residues play an essential role in RFX activity

141 ixth membrane-spanning domains have multiple leucine residues potentially similar to leucine-heptad m

142 -directed mutation of Leu-219, the conserved leucine residue present in all BH3 domains, markedly sup

143 Pases replacement of phenylalanine 28 with a leucine residue produces a "fast cycling" mutant that ca

144 aaX motif is altered to contain a C-terminal leucine residue (Ras-CVLL).

145 d that substitution of isoleucine-100 with a leucine residue resulted in trimethoprim resistance.

146 stitutions in the single and double heptadic leucine residue(s) of melittin, respectively, whereas th

147 retch of basic residues followed by a run of leucine residues spaced seven amino acids apart.

148 56A, where in the P. denitrificans oxidase a leucine residue substituted for the corresponding residu

149 gineered mutations to generate proteins with leucine residues substituted individually for each of th

150 r, S(2), was 0.83-0.96 for all (15)N-labeled leucine residues, suggesting a restricted internal motio

151 ), was unaffected by mutation of the REG/MTS leucine residues, suggesting that activity loss was not

152 binding and bending, and have mutagenized a leucine residue that contacts an adenine nucleotide at t

153 ed protein sequences to identify a conserved leucine residue that is present in nearly all known PCI

154 ly, we investigated the methyl groups of two leucine residues that belong to the hydrophobic core (L1

155 Two conserved leucine residues that contact each face of the adenine r

156 in (DBD) and a mutant lacking two C-terminal leucine residues that form part of the hydrophobic core

157 as altered by site-directed mutagenesis to a leucine residue, the mutant enzyme showed increased subs

158 Mutations at the two conserved leucine residues to alanine at the NES motif (L98A,L100A

159 he tertiary structure from three nonadjacent leucine residues to form a motif reminiscent of establis

160 At higher temperatures, all three labeled leucine residues undergo rapid reorientation about the C

161 This novel NES does not contain multiple leucine residues unlike other CRM1-interacting NES, is c

162 l deuteration of the isopropyl group in each leucine residue was used to probe the orientation and dy

163 zed order parameters for 11 methyl groups of leucine residues were also determined.

164 These leucine residues were also required for optimal viral in

165 ositive rate of only 6%, when isoleucine and leucine residues were not distinguished.

166 ed the hydrogen bonds, disulfide bond, and a leucine residue which may restrict solvent access, by mu

167 A is corroborated by mutagenesis of selected leucine residues, which leads to the abrogation of oligo

168 se factor 1, the UAGA quadruplet specifies a leucine residue with an efficiency of 13 to 26 %.

169 based molecular bridge joins the methyl of a leucine residue with the methyl of its closest lactic ac

170 this region, replacement of any of the three leucine residues with alanine destroyed the function of

171 Replacing six hydrophobic core leucine residues with alanines results in a destabilizat

172 se residues are replaced with isoleucine and leucine residues with branched side chains that are intr

173 s for Fmoc-selenomethionine and substituting leucine residues with selenomethionine.

174 family have been synthesized, replacing core leucine residues with smaller and less hydrophobic resid

175 e mutation, L125V, which affects a conserved leucine residue within the bHLH region.

176 eus localized, and a mutation in a conserved leucine residue within the first helix of the protein in

177 Mutagenesis of two leucine residues within a conventional di-leucine motif

178 Mutation of the conserved tyrosine or leucine residues within the FcepsilonRI beta or gamma IT

179 The conserved leucine residues within the L(D/E)XLLXXL motif were esse

180 genesis was used to selectively mutate eight leucine residues within the leucine zipper motif.

181 Point mutations of specific leucine residues within the NES disrupt the normal subce

182 parin or the receptor, but rather the lysine-leucine residues within the sequence indirectly stabiliz

183 cally, the introduction of helix-stabilizing leucine residues within the TMD region spanning the vesi

184 ther elucidate the role of this region, four leucine residues within this region of Tat were mutated.