ライフサイエンスコーパス: ligation

1 rictions in selenocysteine-mediated chemical ligation.

2 e XLF and Xlf(-/-) mice require PAXX for end-ligation.

3 lems associated with inter-adaptor annealing/ligation.

4 tment to prevent VRB using beta-blockers and ligation.

5 s require ATM/DNA-PK kinase activity for end-ligation.

6 ualized in mice subjected to coronary artery ligation.

7 P-complex spliceosome immediately after exon ligation.

8 t ligation, and 3-fold greater 2 weeks after ligation.

9 sponse to target cell engagement or receptor ligation.

10 s this disulfide bond to allow covalent heme ligation.

11 on, indicating a defect proximal to receptor ligation.

12 -splice site docking, thereby promoting exon ligation.

13 trol group was treated with nadolol+nitrates+ligation.

14 mice subjected to transient coronary artery ligation.

15 n of synthetic liposomes via native chemical ligation.

16 a possible basis for mRNA release after exon ligation.

17 insight into the catalytic mechanism of exon ligation.

18 ignaling events following chemokine receptor ligation.

19 sly subjected to a unilateral femoral vessel ligation.

20 phase oligomer synthesis and the Staudinger ligation.

21 and maturation independently of surface TLR2 ligation.

22 ownstream of target cell engagement or NKp30 ligation.

23 increased significantly following bile-duct ligation.

24 flow restoration in a model of rat hind limb ligation.

25 lassical monocytes required additional notch ligation.

26 oken DNA, thereby stimulating intermolecular ligation.

27 emale and male mice after permanent coronary ligation.

28 subjected to MI by means of coronary artery ligation.

29 o separate murine models: CCl4 and bile duct ligation.

30 in vitro transendothelial migration by CD177 ligation.

31 rotein engineering method, expressed protein ligation.

32 and further enhances signaling following BCR ligation.

33 sesterification reactions-branching and exon ligation.

34 of oligonucleotides into a gene by click-DNA ligation.

35 were subjected to permanent coronary artery ligation.

36 weight 813 [183] grams), 184 (24%) underwent ligation.

37 tion enzyme targeting and two-step proximity ligation.

38 ent left anterior descending coronary artery ligation.

39 natal morbidities arising before exposure to ligation.

40 the 2 MI models (native T1 day 1: permanent ligation, 1280.0+/-162.6 ms; ischemia/reperfusion, 1115.

41 tricular ejection fraction day 21: permanent ligation, 24.5+/-7.0%; ischemia/reperfusion, 33.7+/-11.6

42 Dectin 1 ligation accelerated the progression of PDA in mice, whe

43 In contrast, the protein-protein ligation activity in the original wild-type OaAEP1 enzym

44 ell as structural features important for its ligation activity.

45 of this method, activity-dependent proximity ligation (ADPL), to serine hydrolase and cysteine protea

46 Sortase A-catalysed ligation also obviously improved Tms and product yields

47 ve associated patent ductus arteriosus (PDA) ligation among preterm infants with adverse neonatal out

48 Patent ductus arteriosus ligation among preterm neonates younger than 28 weeks ge

49 e Cu(I)-catalyzed azide-alkyne cycloaddition ligation and by biomimetic formation of a native pattern

50 Proximity ligation and coimmunoprecipitation assays confirmed thro

51 Furthermore, we combined Staudinger ligation and copper catalyzed azide alkyne cycloaddition

52 lic phosphate which protects RNA(3'-rA) from ligation and further deadenylation.

53 r example, distance-dependent random polymer ligation and GC content and mappability bias-and model z

54 y new techniques combining nuclear proximity ligation and high-throughput sequencing, but independent

55 way of activation is initiated by N-cadherin ligation and involves the cadherin coreceptor Cdo with i

56 and assembled using combined native chemical ligation and isonitrile-mediated activation strategies.

57 lineN functions, synthetic aspects of oxime ligation and methodologies for introducing the aminooxy

58 Adult Wistar rats underwent cecal ligation and perforation (CLP), and serum and brain (hip

59 The sepsis group was subjected to a cecal ligation and perforation technique, whereas the control

60 subjected to abdominal surgery without cecal ligation and perforation.

61 ll enzymatic activity for peptide-to-peptide ligation and protein modification, and was successfully

62 CMs exposed to C5a in vitro and after cecal ligation and puncture (CLP) in vivo In both cases, C5a i

63 RP3 in polymicrobial sepsis induced by cecal ligation and puncture (CLP).

64 e at 20 h after induction of sepsis by cecal ligation and puncture (CLP).

65 ubjected to LPS (2 mg/kg) or sepsis by cecal ligation and puncture (CLP).

66 d 2 stringent animal models of sepsis: cecal ligation and puncture as well as intraperitoneal injecti

67 Wild-type mice made septic via cecal ligation and puncture had decreased crypt proliferation

68 l titers compared with vehicle-treated cecal ligation and puncture mice that succumbed at 48 h.

69 -12,255) would improve survival in the cecal ligation and puncture model of sepsis in adult female ou

70 icantly increased survival in a murine cecal ligation and puncture model.

71 oduction during septic shock caused by cecal ligation and puncture or endotoxin-induced shock.

72 Sprague-Dawley rats were randomized to cecal ligation and puncture or sham surgery.

73 demonstrated increased survival after cecal ligation and puncture relative to mice receiving low-fib

74 tiation of sepsis improved survival in cecal ligation and puncture sepsis (neurokinin-1 receptor anta

75 n LTB4 production in vivo and in vitro Cecal ligation and puncture surgery was performed in mice, wit

76 lso exacerbated septic shock caused by cecal ligation and puncture when given after the onset of seps

77 ting WBCs, augment the early (6 hr postcecal ligation and puncture) recruitment of inflammatory cells

78 elevant model of polymicrobial sepsis (cecal ligation and puncture), DJ-1(-/-) mice had improved surv

79 Twenty rodents (10 cecal ligation and puncture, 10 sham) were killed at 24 hours,

80 Mice were also subjected to cecum ligation and puncture, a model used to induce peritoneal

81 In polymicrobial sepsis initiated by cecal ligation and puncture, RvD2 ( approximately 2.7 nmol/mou

82 e exhibited enhanced susceptibility to cecal ligation and puncture- and LPS-induced sepsis, which cor

83 collagen elastic modulus was lower in cecal ligation and puncture-exposed rats at 24 hours (1.37 +/-

84 al neck were significantly reduced for cecal ligation and puncture-exposed rodents at 24 hours (83.39

85 was similar at 24 hours but reduced in cecal ligation and puncture-exposed rodents at 96 hours (75.34

86 lear cells rescued wild-type mice from cecal ligation and puncture-induced mortality.

87 Upon lipopolysaccharide or cecal ligation and puncture-induced peritonitis, these margina

88 of iCRT3 to C57BL/6 mice, subjected to cecal ligation and puncture-induced sepsis, decreases the plas

89 ntra-peritoneal lipopolysaccharide and cecal ligation and puncture.

90 were subjected to 45 min of coronary artery ligation and reperfusion for 12 weeks.

91 inical PAD models (unilateral femoral artery ligation and resection) were conducted to examine the ro

92 n with RNase E, allowing proximity-dependent ligation and sequencing of cognate sRNA-mRNA pairs as ch

93 exes can be recovered using UV-crosslinking, ligation and sequencing of hybrids (CLASH).

94 atient underwent urgent right hepatic artery ligation and splenectomy.

95 l click approach with a combination of oxime ligation and strain promoted alkyne-azide cycloaddition.

96 ha in murine carbon tetrachloride, bile duct ligation, and 0.1% 3,5-diethoxycarbonyl-1,4-dihydrocolli

97 s was 2-fold greater in shams at 1 week post ligation, and 3-fold greater 2 weeks after ligation.

98 acked information on 3'-ss recognition, exon ligation, and exon release.

99 se chain reaction, isothermal amplification, ligation, and hybridization reactions.

100 on after partial hepatectomy and portal vein ligation, and increased the expression of cell cycle-pro

101 which a target sequence templates a chemical ligation, and the product of this reaction is in turn de

102 ins eight triazoles at the sites of chemical ligation, and yet is fully biocompatible; it is replicat

103 s and glycodendrimers obtained through oxime ligation are described in terms of synthetic design and

104 mpatibilities, including products of aborted ligation, are sufficient to disrupt end pairing.

105 t comparable to fluorine and introduce oxime ligation as a tool for the functionalization of syntheti

106 Co-immunoprecipitation and proximity ligation assay (Duolink) indicated that PTPRO directly p

107 Proximity Ligation Assay (PLA) demonstrated that HP1a is in close

108 method, the polyclonal serum-based proximity ligation assay (polyPLA), to identify antigenic variants

109 by a Triton solubility assay and a proximity ligation assay aiming to measure interactions between NM

110 C, G190A, M184 V, or K65R by oligonucleotide ligation assay and Illumina sequencing.

111 A proximity ligation assay confirmed the endogenous Trx1-GC1 complex

112 Proximity ligation assay revealed direct interactions between SNAP

113 ys, immunoblotting, and an in situ proximity ligation assay to characterize the truncated NEMO protei

114 By combining proximity ligation assay with chemical labeling of cysteine residu

115 Using an optimized adaptor ligation assay, we found that most T. brucei telomere G-

116 blotting, immunofluorescence, and proximity ligation assay.

117 pairs of antibodies using in situ proximity ligation assays (PLA).

118 s of these compounds, we performed proximity ligation assays in endothelial cells (ECs) to demonstrat

119 Using co-immunoprecipitation and proximity ligation assays on endogenous proteins, we demonstrate t

120 mistry, co-immunoprecipitation and proximity ligation assays revealed that store depletion activated

121 Proximity ligation assays show that these 2 proteins are physicall

122 lso occurred in cellulo as tested by protein ligation assays; this interaction is inhibited by triflu

123 strate activation by ATP to perform C-C bond ligation at a distant Mn center.

124 Genome-wide proximity ligation based assays like Hi-C have opened a window to

125 Genome-wide proximity ligation based assays such as Hi-C have revealed that eu

126 yperplasia was induced in rats via bile duct ligation (BDL) surgery, and galanin was increased in ser

127 MCs infiltrate the liver following bile duct ligation (BDL), increasing intrahepatic bile duct mass (

128 nesis of liver fibrosis induced by bile duct ligation (BDL).

129 ral cortex using rat models of HE (bile duct ligation [BDL] and induced hyperammonemia) and also eval

130 epetitive switch region elements followed by ligation between distal breaks.

131 mation of a deleterious by-product, reducing ligation bias, and improving read coverage.

132 Slu7 and Prp18, which promote exon ligation, bind together to the Prp8 RNase H-like domain.

133 rt functional recovery after coronary artery ligation both correlated with pre-injury MNDCM content.

134 Pembrolizumab prevents PD-1 ligation by both PD-L1 and PD-L2, preventing the immune

135 ciated with altered CCR7 signaling following ligation by CCL19, a receptor:ligand interaction critica

136 BCR ligation by endogenous or exogenous ligands induced the

137 The ligation by four deprotonated amide nitrogens in macrocy

138 and thermodynamics of single-stranded break ligation by LIG3alpha and LIG3beta and compared this fra

139 ) and show only LAMP-2 is internalized after ligation by specific Abs, including H4B4, and traffics r

140 3-hydroxyl DNA termini that are critical for ligation by the NHEJ DNA ligase, LigIV.

141 B cells required Taok3 to rapidly respond to ligation by the Notch ligand Delta-like 1.

142 tial transesterifications-branching and exon ligation-catalysed at a single catalytic metal site in U

143 ell penetrating peptide, using bioorthogonal ligation chemistry.

144 r genome, scaffolded with in vitro proximity ligation (Chicago) data.

145 mong the apocytochrome c, CcmG, and the heme-ligation components CcmF, CcmH, and CcmI.

146 otgun Illumina reads plus in vitro proximity ligation data to create large superscaffolds; it was val

147 t have a decreased cytotoxic capacity, 4-1BB ligation demonstrates a potent ability to restore effect

148 cells, exploiting a combination of multiplex ligation-dependent probe amplification (MLPA) and electr

149 Multiplex ligation-dependent probe amplification (MLPA) did not re

150 analyses by direct sequencing and multiplex ligation-dependent probe amplification and retrospective

151 ion methods based on principles of multiplex ligation-dependent probe amplification and targeted MS/M

152 es and bacteria were identified by multiplex ligation-dependent probe amplification assay and convent

153 er were studied in a FCGR-specific multiplex ligation-dependent probe amplification assay.

154 ional mutation screening underwent multiplex ligation-dependent probe amplification.

155 xquisite site-selectivity of the aza-Michael ligation described herein allows the construction of sta

156 ith this, combining BCR signalling with CD40 ligation did not reduce IL-10 secretion as was observed

157 olecule FRET (smFRET), we show that prior to ligation, differences in end chemistry strongly modulate

158 mediated ligation (PBSL), which improves the ligation efficiency to over 95 % by linking the target p

159 alogous complex with a pincer-type mer-C,N,S ligation, emphasizing the importance of the fac-C,N,S-ir

160 Correspondingly, CD177 ligation enhanced its interaction with beta2 integrins,

161 developed using sortase A (SrtA), a peptide ligation enzyme, as a generic processor.

162 This serves as an example where protonation-ligation events enhance protein-protein interactions fun

163 m-operated mice, mice with HF (8 weeks after ligation) exhibited the following features: (1) signific

164 Additional proximity ligation experiments and enrichment for Sr0161 targets i

165 model of sterile inflammation utilizing TLR4 ligation followed by ATP or nigericin treatment, inflamm

166 dial PBS injections (control group), and LAD ligation followed by NP12 administration (NP12 group).

167 re subjected to in vivo left coronary artery ligation for 30 minutes followed by 72 hours reperfusion

168 extended partial hepatectomy and portal vein ligation for multiple bilobar CRLM were applied to asses

169 Associating Liver Partition and Portal Vein Ligation for Staged Hepatectomy (ALPPS) occurred over ti

170 their target genomic sequences by proximity ligation, forming RNA-DNA chimeric sequences, which are

171 sing standard DNA assembly methods including ligation, Gateway, Gibson, and Golden Gate.

172 c glycan engineering (MGE) and bioorthogonal ligation have enabled study of biological roles of glyca

173 e real-time (SMRT) sequencing, and proximity ligation (Hi-C) sequencing-we show that HapCUT2 rapidly

174 oci reside, as measured by DNA-DNA proximity ligation (Hi-C).

175 s have focused on examining DNA cleavage and ligation; however, the dynamic opening and closing of th

176 ymph nodes and respond to toll-like receptor ligation; however, they differ markedly in their respons

177 estored in 2 to 3 weeks after femoral artery ligation in all groups of mice fed a normal diet.

178 plex and sometimes unexpected outcome of CLR ligation in bacterial and fungal infection.

179 eeds via intermediates that have carboxylate ligation in common, and a secondary cycle involving a bi

180 od volume flow in the carotid arteries after ligation in FVB mice.

181 binding could contribute up to 96% of VEGFR2 ligation in healthy conditions and in cancer.

182 Our data show that TLR4 ligation in LPS-primed DCs, induced higher levels of bot

183 izer) was administered after coronary artery ligation in mice.

184 hypertension was established using bile duct ligation in rats.

185 cribe a broadly applicable technique called 'Ligation in situ Hybridization' ('LISH'), which is an al

186 sting that Mg(2+) availability regulates DNA ligation in vivo, because Mg(2+) levels are higher in th

187 a unifying strategy for chemoselective CuAAC ligations in multialkyne/azide systems.

188 mune responses, and antibodies blocking PD-1 ligation, including pembrolizumab, have recently receive

189 ands are capable of tissue repair after duct ligation-induced injury, likely involving resident stem/

190 odel with lacrimal gland main excretory duct ligation-induced injury.

191 The finding that metal ligation inhibits this energy wasting reaction is of dir

192 We show that CR3 ligation initiates a complex mechanism of integrin cross

193 s preferentially bound by E-selectin and, on ligation, initiates secretion of MRP8/14 that binds TLR4

194 The ligation-injured lacrimal glands temporarily decreased i

195 s attenuated in mouse carotid arteries after ligation injury and in failed human arteriovenous fistul

196 ted cell-level computational model of VEGFR2 ligation, intracellular trafficking, and site-specific p

197 IIalpha-XRCC1-nucleosome complex decays when ligation is complete, allowing the host nucleosome to re

198 he necessary transitions from end pairing to ligation is not known.

199 Selenocysteine-mediated ligation is noteworthy for its high yield and efficiency

200 iels-Alder cycloaddition, that is, tetrazine ligation, is reported.

201 48 hours following IVC ligation, IVC thrombosis was evident in 60% of WT mice a

202 Furthermore, using telomere oligo ligation-mediated PCR, we showed for the first time that

203 xtended the use of the in vivo RNA proximity ligation method toward probing global sRNA interactions

204 t ventricular ejection fraction in permanent ligation mice.

205 hifted from acetylide formation to the azide ligation/migratory insertion event allowing chemoselecti

206 erior communicating artery aneurysms by clip ligation (n = 14) or coiling (n = 9).

207 ent left anterior descending coronary artery ligation (n=14) or 50 minutes ischemia/reperfusion (n=13

208 operation of the machine via native chemical ligation (NCL) demonstrates that even challenging 15- an

209 sequence in order to perform native chemical ligation (NCL) of two peptide fragments.

210 Three days after ligation, neutrophil infiltration into ischemic limbs of

211 of chronic liver injury, including bile duct ligation, nonalcoholic steatohepatitis, and obese mice,

212 rotein XRCC1 enhances the ligation; that the ligation occurs within a complex that ligase IIIalpha-XR

213 oseryl-tRNA synthetase (SepRS) catalyzes the ligation of a mismatching O-phosphoserine (Sep) to tRNA(

214 acrocycle with substrate atoms and the axial ligation of adducts.

215 1(low) phenotype and a defective response to ligation of BCR and Toll-like receptor 9 (TLR9).

216 Specific ligation of C5aR2 in neutrophils from tdTomato-C5aR2 mic

217 Ligation of Dectin-1 by fungal glucans elicits a Th17 re

218 Da CD4 miniprotein mimetic, serving to block ligation of gp140 on CD4(+) T cells while preserving CD4

219 cross-linking immunoprecipitation (CLIP) and ligation of miRNA-target chimeras on the Argonaute (AGO)

220 ir shortcomings; enzymatic amplification and ligation of oligonucleotides into genes and genomes make

221 Ligation of OX40 L and JAG1 with their cognate-receptors

222 Ligation of programmed cell death-1 (PD-1) in the tumor

223 reaction environment to achieve an efficient ligation of protein domains independently from their str

224 al biology due to their ability to drive the ligation of separately expressed polypeptides through a

225 icrobial pathogens leads to the simultaneous ligation of several CLRs and TLRs, the signals emanating

226 Co-ligation of SIRPalpha with CD47 abrogates nanocluster se

227 (sham group), left anterior descending (LAD) ligation of the coronary artery followed by intramyocard

228 the pathway necessary for the synthesis and ligation of the modification is unknown.

229 Rats subjected to unilateral partial ligation of the sciatic nerve developed significant mech

230 We determined that ligation of the synergy site of FN permits tumor cells t

231 We demonstrate that upon ligation of the T-cell antigen receptor (TCR), the TCR a

232 the specific RBP using immunoprecipitation; ligation of the two arms of RNA duplexes via a linker; r

233 Ligation of these death receptors can also induce necrop

234 We demonstrate that ligation of TLR3, TLR4, and TLR9 induces murine DC produ

235 strain, we found that B cell receptor (BCR) ligation of Ubqln1(-/-) B cells led to a defect in cell

236 eters (9.6% vs 3.9%; P = .03), repair and/or ligation of vascular injury (15.1% vs 5.4%; P = .001), c

237 ters (16.1% vs 5.5%; P = .02), repair and/or ligation of vascular injury (54.8% vs 25.3%; P < .001),

238 h multivariable analysis, only repair and/or ligation of vascular injury (OR, 3.32; 95% CI, 1.37-8.03

239 a functional handle required for Staudinger ligation on solid support.

240 4-1BB ligation on T cells triggers a signaling cascade that re

241 wley dams underwent bilateral uterine artery ligation or anesthesia (control) at e19.5.

242 as administered to rats with coronary artery ligation over a period of six weeks.

243 cholestatic liver injury, partial bile duct ligation (pBDL), with a novel longitudinal bioimaging me

244 developing proximity-based sortase-mediated ligation (PBSL), which improves the ligation efficiency

245 re we used long-term partial pancreatic duct ligation (PDL) as a model to study CPRD.

246 molecule sequencing, can be used to analyze ligation products from complex panels of LISH probes ('L

247 reatment with RNase H releases RNA-templated ligation products into solution for downstream analysis.

248 ELA-treated cecal ligation puncture rats were the only group to 1) display

249 ed cardiac dysfunction was produced by cecal ligation puncture to assess hemodynamic efficacy, cardio

250 linergic agonist infusion counteracted cecal ligation puncture-induced myocardial dysfunction by impr

251 ns, the two enzymes had very similar maximal ligation rates.

252 Following the ligation reaction, SpyTag is cleaved off, rendering PBSL

253 pendent conversion of two rapid photoinduced ligation reactions, i.e., the light activation of o-meth

254 yme catalyzes site-specific RNA cleavage and ligation reactions.

255 depletion in HF mice (starting 4 weeks after ligation) reduced cardiac infiltration of CD4(+) T cells

256 ter femoral artery or middle cerebral artery ligation, respectively.

257 py performed two hours following partial IVC ligation revealed that leukocyte firm attachment was inc

258 CD177 ligation selectively impaired migration of CD177(pos) ne

259 Ligation sessions were repeated until response was achie

260 n were also amenable to traceless Staudinger ligations, setting the stage for tandem labeling experim

261 e, selenocysteine-mediated expressed protein ligation simplifies incorporation of post-translational

262 zed/carbonylated protein has a vacant distal ligation site, and it represents the true peroxidase-act

263 athic pain behaviors in the rat spinal nerve ligation (SNL) model.

264 rons is downregulated following spinal nerve ligation (SNL).

265 Sortase-mediated ligation ("sortagging") of LPXTG-containing substrates a

266 In a mouse model, DVT was induced by partial ligation (stenosis) of the inferior vena cava.

267 Here we report that the DNA ligation step of BER is compromised after pol beta inser

268 lified chromatin fragmentation and proximity ligation step that eliminates bead purification and wash

269 s often require poly(A) enrichment, 3 linker ligation steps, and RNA fragmentation, which can necessi

270 To address this, and to develop new chemical ligation strategies for assembly of biocompatible modifi

271 We present a simple single tube proximity ligation technique, targeted chromatin ligation, that ca

272 d explain why LIG3 is less prone to abortive ligation than LIG1.

273 imity ligation technique, targeted chromatin ligation, that captures histone modification patterns wi

274 t the scaffolding protein XRCC1 enhances the ligation; that the ligation occurs within a complex that

275 With TLR ligation, the deubiquitinase enzyme, STAM-binding protei

276 Following ligation, the selenocysteine can be deselenized into an

277 tality was lower among infants who underwent ligation, though residual survival bias could not be exc

278 of effector cell activation requires direct ligation to an activating FcepsilonRI receptor.

279 s underwent partial inferior vena cava (IVC) ligation to induce venous thrombosis.

280 We used native chemical ligation to synthesize the grafted cyclic peptide.

281 rying a T-tail at the 3' end), for efficient ligation to target DNA and subsequent PCR amplification

282 In contrast, selective ligation to the other sulfur-containing amino acid, meth

283 (with or without ischemia produced by iliac ligation) to therapeutic ultrasound (1.3 MHz, mechanical

284 es that even challenging 15- and 19-membered ligation transition states are suitable for information

285 ound that in mice with partial sciatic nerve ligation, TRPA1 silencing in nociceptors attenuated mech

286 Evaluate the association between PDA ligation vs medical management and neonatal and neurodev

287 Ligation was associated with lower odds of mortality (aO

288 DC processing in the absence of surface TLR2 ligation was defined using synthetic virus-like particle

289 Recovery of blood flow after femoral artery ligation was impaired (>80%) in AMPKalpha2(-/-) versus w

290 METHODS AND Permanent coronary ligation was performed in adult C57BL/6 mice.

291 The products of the cyclopropenone ligation were also amenable to traceless Staudinger liga

292 t synthetic analogue with biomimetic {SN/S2} ligation which could be structurally characterized in it

293 ic pain was induced by partial sciatic nerve ligation (which induces hypersensitivity to mechanical,

294 o ligate two protein fragments, but multiple ligations, which are necessary to segmentally label prot

295 fragmentation by sonication, ChIP, proximity ligation with a bridge linker, Tn5 tagmentation, PCR amp

296 Disruption of integrin ligation with an RGD-containing peptide abrogated IL-13-

297 lanced reactivity and stability in tetrazine ligation with the protein substrates.

298 Most current approaches rely on multi-step ligations with low yields and incomplete products.

299 ng the conjugation site in expressed protein ligations with no influence on native cysteines.

300 These components undergo rapid tetrazine ligation within the plasma membrane to generate the HIDE