ライフサイエンスコーパス: ligation reaction

1 opies of itself through an RNA-catalyzed RNA ligation reaction.

2 circularized in a strictly target dependent ligation reaction.

3 Lys251 and Asp253 at different steps of the ligation reaction.

4 random sequences, performs an efficient RNA ligation reaction.

5 ne functional groups to the hairpin ribozyme ligation reaction.

6 t concentrations as high as 50 microM in the ligation reaction.

7 required for the strand closure step of the ligation reaction.

8 ing, at the end distal to the join, slow the ligation reaction.

9 -mRNA splicing before the first cleavage and ligation reaction.

10 mes in which decarboxylation is coupled to a ligation reaction.

11 emical evidence has been established for the ligation reaction.

12 oach that enabled the reconstitution of this ligation reaction.

13 -5'-AMP), the obligatory intermediate of the ligation reaction.

14 ucidation of the mechanism of WaaL-catalyzed ligation reaction.

15 ereo-specificity of the heme-apocytochrome c ligation reaction.

16 ynthesized peptide using the native chemical ligation reaction.

17 and catalytic mechanism of the kinetoplastid ligation reaction.

18 riction-enzyme-digested vectors prior to the ligation reaction.

19 sion through the three chemical steps of the ligation reaction.

20 ing RNAs because the cP inhibits the adapter ligation reaction.

21 ined by Watson-Crick base pairing during the ligation reaction.

22 inhibition of the hairpin-catalyzed RNA-RNA ligation reaction.

23 ion or phosphodiester synthesis steps of the ligation reaction.

24 nd 3'-ends of the RNA into proximity for the ligation reaction.

25 yme catalyzes site-specific RNA cleavage and ligation reactions.

26 ctive groups on protein sequences for use in ligation reactions.

27 ure have been examined in the BrCN activated ligation reactions.

28 xhibited quite different effects on the same ligation reactions.

29 omains is required for both the cleavage and ligation reactions.

30 late groups from DNA that arise from aborted ligation reactions.

31 e labeling using in vitro selective chemical ligation reactions.

32 e regioselectivity of ribozyme-catalyzed RNA ligation reactions.

33 icks or breaks that result from abortive DNA ligation reactions.

34 ithout the need for restriction digestion or ligation reactions.

35 f miRNA-target chimeras formed by endogenous ligation reactions.

36 ions has classically focused on bond-forming ligation reactions.

37 DNA sites and catalysis of DNA cleavage and ligation reactions.

38 e for sequence-specific primer extension and ligation reactions.

39 In the ligation reaction, a 2'-5' RNA phosphodiester linkage is

40 Unexpectedly, ligation reactions also occurred in the absence of the e

41 f this reaction limits the efficiency of the ligation reaction and has become a significant constrain

42 t compared to their effects on the composite ligation reaction and individual upstream steps.

43 uorescence protein (GFP) through an in vitro ligation reaction and the 17.8-kb-long X-inactive-specif

44 ibuting to the remarkable specificity of the ligation reaction and the physiological reaction conditi

45 al domain that executes the DNA cleavage and ligation reactions and a smaller amino-terminal domain t

46 uential cleavage, U addition or removal, and ligation reactions and is directed by complementary guid

47 luded the standard enzyme digestion-mediated ligation reactions and the subsequent isolation of plasm

48 DNA fragments in ligation reactions are capable of combining to produce n

49 The tyrosine ligation reactions are shown to be compatible with the l

50 ere extended by further amino acid residues, ligation reactions became slower.

51 addition to oligosaccharide sequencing, the ligation reaction between an oligosaccharide and an amin

52 s model is based on the L-21 Sca I catalyzed ligation reaction between exogenously added oligomers: c

53 Monitoring probe hybridization and ligation reactions by electrophoretic mobility retardati

54 limitations of the canonical native peptide ligation reaction catalyzed by sortase A.

55 porated into the cell wall through a peptide ligation reaction catalyzed by transpeptidase sortase.

56 repair pathway, namely the DNA synthesis and ligation reactions catalyzed by E. coli DNA polymerase a

57 We have found that the rate of the trans ligation reaction depends on pH, corresponding to the pr

58 ible; the pyrophosphate leaving group in the ligation reaction does not induce 2',5'-cleavage, and py

59 ults revealed that IntDOT is able to perform ligation reactions even when all the bases within the cr

60 e C-C cross-link does not interfere with the ligation reaction, even when it is located only two base

61 ternal" equilibrium between the cleavage and ligation reactions for the circular hammerheads was shif

62 ntacts and conformational changes propel the ligation reaction forward.

63 The native chemical ligation reaction has been used extensively for the synt

64 A template-free click-ligation reaction has been used for the intramolecular c

65 This ligation reaction has similarities to the reaction catal

66 It is based on the use of a specific gap ligation reaction, horseradish peroxidase (HRP) for sign

67 DNA ligases catalyze a NAD(+)-dependent DNA ligation reaction, i.e., the formation of a phosphodiest

68 pendent conversion of two rapid photoinduced ligation reactions, i.e., the light activation of o-meth

69 d to a larger total surface area for the RNA ligation reaction; (ii) the SiNPs enhance the diffusion

70 s that theoretically may both participate in ligation reactions, implying that potentially not only p

71 L), combines the PCR and the oligonucleotide ligation reaction in a two-stage thermal cycling sequenc

72 ormations that may help us to understand the ligation reaction in FPGS and influence the design of me

73 is catalyzed by the ribozyme catalyzing the ligation reaction in its deprotonated state (rate 1.05 m

74 orming a truncated helix 7 that promotes the ligation reaction in vitro.

75 Upon transformation of the ligation reaction into Escherichia coli, infectious phag

76 alf of the selected deoxyribozymes mediate a ligation reaction involving the natural branch-point ade

77 7, the equilibrium constant (K(eq)) for the ligation reaction is 3.89 x 10(4) m.

78 The Mn2+-dependent branch-forming ligation reaction is between an internal branch-site 2'-

79 hioalkyl esters are rather unreactive so the ligation reaction is catalyzed by in situ transthioester

80 The ligation reaction is effectively irreversible; the pyrop

81 A self-catalyzed mechanism for this cleavage-ligation reaction is presented, based on mutagenesis dat

82 The requirement of 3' complementarity for a ligation reaction is reaffirmed by results from 1 nt ins

83 The SrtA-mediated ligation reaction is reversible, so most labeling protoc

84 as the conserved peptide, whose role in the ligation reaction is unknown.

85 a rapid, copper-free, tetrazine-cyclopropene ligation reaction (k2 > 5 M(-1) s(-1)).

86 ntly attached to the surface by an enzymatic ligation reaction (leaving the anti-sense strand dissoci

87 In this method, DNA hybridization and ligation reactions led to the attachment of ATRP initiat

88 m and the tight coupling of the cleavage and ligation reactions make it difficult to characterize the

89 of uncatalyzed RNA backbone cleavage, their ligation reactions may be of direct relevance to the RNA

90 catalytic determinants for the cleavage and ligation reactions mediated by the hairpin ribozyme are

91 Treatment of the ligation reaction mixture with exonuclease prior to ampl

92 3' A overhang by including 0.5 M NaCl in the ligation reaction mixture.

93 of peptide or protein products directly from ligation reaction mixtures by Ni-NTA affinity column pur

94 nous nucleobase rescue for both cleavage and ligation reactions now allow us to refine models of the

95 functionalized sialic acid derivative with a ligation reaction of a fluorogenic tetrazine, allowing f

96 +)-dependent deoxyribozymes that mediate the ligation reaction of an RNA 5'-hydroxyl group with a 2',

97 The DNA cleavage-ligation reaction of DNA topoisomerase I was investigate

98 A bis-ligation reaction of S-nitrosothiols using triaryl subst

99 ificant roles in modulating the DNA cleavage/ligation reaction of the enzyme and its response to anti

100 Optimum pH for the ligation reaction of the human telomere sequence ranges

101 familiarize the reader with the DNA cleavage/ligation reaction of topoisomerase II and other aspects

102 The DNA ligation reaction of topoisomerase II is essential for g

103 The DNA cleavage/ligation reaction of topoisomerase II is the target for

104 e found that nonenzymatic, template-directed ligation reactions of oligoribonucleotides display high

105 were investigated by comparing cleavage and ligation reactions of ribozyme variants with A38 modific

106 However, simple RNA self-cleavage and ligation reactions offer a unique opportunity to measure

107 However, optimizing such multi-parametric ligation reactions often involves extensive trial and er

108 In principle, performing the ligation reactions on a solid support would eliminate th

109 ing (SPRI) measurements of surface enzymatic ligation reactions on DNA microarrays is demonstrated.

110 method has been used to analyze the multiple ligation reactions onto radiolabeled DDAH 4PteGlu 1 cata

111 hairpin ribozyme-mediated self-cleavage and ligation reactions participate in processing RNA replica

112 lization and a general base may catalyze the ligation reaction required for prebiotic RNA assembly.

113 Following the ligation reaction, SpyTag is cleaved off, rendering PBSL

114 Analysis of directionality of the ligations reactions suggests that for each of the Thermu

115 Cl(2)/H(2)O medium proved to be best for the ligation reactions, suppressing the undesired azide redu

116 activity was found to be required for an end-ligation reaction that circularizes a portion of the uni

117 zation, an efficient one-step chemical sugar ligation reaction that does not require prior sugar prot

118 ation of exogenous lipoate is catalyzed by a ligation reaction that proceeds via a lipoyl-adenylate i

119 to highly specific self-cleavage and protein ligation reactions that are useful protein engineering t

120 ck" is often applied inaccurately to polymer ligation reactions that fail to respect the criteria tha

121 res are two sequential pairs of DNA cleavage/ligation reactions that proceed via a 3' phosphotyrosine

122 moval of adenylates that arise from abortive ligation reactions that take place at incised abasic sit

123 During ligation reactions, the donor fluorophore europium (Eu(3

124 g ligated products in template-promoted self-ligation reactions, thus yielding multiple signals per t

125 adily by camptothecin than the corresponding ligation reaction to form a fully complementary duplex;

126 the acceptor strand was not required for the ligation reaction to proceed, but duplex formation to pr

127 ites coupled with simultaneous digestion and ligation reactions to create just one product, by conver

128 Despite the use of these thiol catalysts, ligation reactions typically take 24-48 h.

129 aryotic genomes is ensured by three-step DNA ligation reactions used by ATP-dependent DNA ligases.

130 the bisphosphate and carried out successive ligation reactions using T4 RNA ligase and T4 DNA ligase

131 The relative observed rate of the ligation reaction was a minimum of 35 times faster on th

132 The ligation reaction was carried out using an N-terminal re

133 hat the activity of human Cdc34 in the Ub-Ub ligation reaction was enhanced dramatically by SCF's cor

134 omerase I-mediated mismatch formation in the ligation reaction was inhibited more readily by camptoth

135 The ligation reaction was rapid for a broad range of substra

136 nt base ionization events in the VS ribozyme ligation reaction, we performed nucleotide analogue inte

137 bstitution, the facility of the cleavage and ligation reactions were altered.

138 nkages accompanying all 16 stepwise cyanomet ligation reactions were experimentally resolved, only tw

139 Both ligation reactions were performed in aqueous buffered so

140 s for ATP and DNA substrates, in the overall ligation reaction, were 0.4 microM and 30 nM, respective

141 The nonenzymatic ligation reactions, which are characteristic of base pai

142 This efficient DNA ligation reaction will facilitate development of robust

143 ctrodes apparently because of a slow nitrite ligation reaction with Rh(III); however, a significant i

144 lycosylated residue were shown to facilitate ligation reactions with peptide thioesters, and these pr

145 Comparison of oligonucleotide ligation reactions with previously characterized single

146 the polymer film and their strength of axial ligation reactions, with a selectivity pattern of 1-buty

147 Arg-54 and Lys-119 abolished the overall RNA ligation reaction without affecting steps 1 and 3.

148 This mild aqueous tyrosine ligation reaction works over a broad pH range and expand