ライフサイエンスコーパス: limbal

1 (29%) underwent living-related conjunctival limbal allograft and 10 eyes (71%) underwent cadaver-don

2 ance with impression cytology, and to detect limbal alterations associated with partial or total conj

3 epithelial stem cells, are detected in both limbal and central cornea of mouse eye.

4 The limbal and central corneal epithelial cells of 6-week-ol

5 The rat limbal and central corneal epithelial SAGE libraries con

6 The basal layer of limbal and central corneal epithelium is enriched in ste

7 Side-population (SP) cells isolated from limbal and conjunctival epithelia derive from cells that

8 em cells are present throughout the corneal, limbal and conjunctival epithelia.

9 the stratified but non-keratinized corneal, limbal and conjunctival epithelium, in concert with the

10 Analyses of RNA isolated from 300 limbal and corneal basal cells from eight mice revealed

11 odissection, we were able to isolate resting limbal and corneal basal cells from frozen sections with

12 Within the limbal and corneal basal cells, we detected previously u

13 n of transcriptional coactivator YAP1 in the limbal and corneal basal epithelial cells and its essent

14 Limbal and corneal epithelia from wild-type FIH-1(-/-) a

15 ip between FIH-1 and c-kit as it pertains to limbal and corneal epithelial glycogen stores.

16 ly in CD-1 mice by laser photocoagulation of limbal and episcleral veins 270 degrees to 300 degrees c

17 Laser photocoagulation of limbal and episcleral veins induces transient ocular hyp

18 ly in CD-1 mice by laser photocoagulation of limbal and episcleral veins.

19 sed the numbers of gammadelta T cells in the limbal and peripheral corneal epithelium and in the corn

20 ion-PCR confirmed the up-regulation of three limbal and three corneal genes.

21 cular history, best-corrected visual acuity, limbal anterior chamber depth assessment, frequency-doub

22 <7 months of age by the same surgeon using a limbal approach.

23 Ophthalmologic findings included loss of limbal architecture, a whorl-like epitheliopathy, or an

24 noted in the bulbar conjunctiva and near the limbal area of corneas from NK1R(-/-) mice.

25 eity of preexisting lymphatic vessels in the limbal area significantly correlated with the extent of

26 vessels are not evenly distributed in normal limbal areas.

27 c vessels in the nasal side in normal murine limbal areas.

28 ated with physiological parameters, that the limbal attachment can cause small (<20%) increases in TM

29 inner hair cell stimulation, and reveal the limbal attachment of the TM plays a critical role in thi

30 oading effects of hair bundle stiffness, the limbal attachment of the TM, and viscous damping in the

31 ygium excision compared with conjunctival or limbal autograft or mitomycin C alone.

32 tudies demonstrated that the conjunctival or limbal autograft procedure is more efficacious than amni

33 Conjunctival or limbal autograft was superior to amniotic membrane graft

34 The outcomes of conjunctival or limbal autograft were similar to outcomes for intraopera

35 , where pterygium excision with conjunctival-limbal autograft with fibrin glue application was done.

36 that using a combination of conjunctival or limbal autograft with mitomycin C further reduces the re

37 paring bare sclera excision, conjunctival or limbal autograft, intraoperative mitomycin C, postoperat

38 Use of conjunctival or limbal autografts or mitomycin C during or after pterygi

39 vidence that mitomycin C and conjunctival or limbal autografts reduce the recurrence rate after surgi

40 on markers, that is, K3 and K12 keratins, in limbal basal cells (these markers are expressed, however

41 airlift cultures, p38 expression emerged in limbal basal epithelial cells and gradually extended to

42 lusive location of slow-cycling cells in the limbal basal layer, the superior in vitro proliferative

43 To compare effectiveness of fornix- and limbal-based conjunctival flaps in trabeculectomy surger

44 shallow anterior chamber was observed in the limbal-based group.

45 anterior chamber, which was increased in the limbal-based group.

46 In the fornix-based and limbal-based surgery, mean IOP at 12 months was similar,

47 ich benefits and complications of fornix- vs limbal-based trabeculectomy for glaucoma were compared i

48 nce in effectiveness between fornix-based vs limbal-based trabeculectomy surgery, although with a hig

49 , -gamma1, -gamma2 and -gamma3 chains in the limbal basement membrane, with LN-alpha5 representing a

50 t, common culture approaches, outgrowth from limbal biopsy explants and isolated cell seeded in low c

51 with total limbal stem cell deficiency using limbal biopsy explants cultured on intact amniotic membr

52 mary surgery all patients underwent a repeat limbal biopsy from the unaffected eye.

53 omes are similar irrespective of whether the limbal biopsy is taken from the healthy part of the ipsi

54 A 1-clock hour limbal biopsy sample was obtained from the unaffected ey

55 After a limbal biopsy specimen obtained from a healthy area of t

56 The limbal biopsy was taken either from the healthy part of

57 In 34 eyes the limbal biopsy was taken from the ipsilateral eye and in

58 appeared to be involved in the formation of limbal blood vessels and corneal nerve fibers.

59 um and in the corneal stroma adjacent to the limbal blood vessels.

60 corneal limbus and became distributed around limbal blood vessels.

61 In all corneas, limbal BM contained laminin gamma3 chain.

62 2 and beta2 chains were present in the adult limbal BM where epithelial stem cells are located.

63 pha1-alpha2 chains were retained only in the limbal BM.

64 3 years of age, beta2 chain appeared in the limbal BM.

65 and hyperproliferation only happened in the limbal, but not the corneal, epithelium in airlift, but

66 Limbal cells obtained from the superior limbus, isolated

67 0 genes that are differentially expressed in limbal cells versus corneal epithelial basal cells.

68 The limbal cells were expanded ex vivo on a human amniotic m

69 Limbal cells were harvested from healthy or less affecte

70 imbal epithelial cells, and the transplanted limbal cells' ability to reconstitute corneal epithelium

71 istorical controls, including mean number of limbal clock hours affected by OSSN (6 vs 4; P = .12), m

72 single tumor >/=15 mm basal diameter or >/=6 limbal clock-hours) was managed with topical IFNalpha2b

73 Limbal conjunctival autograft was more effective than in

74 eyes) with sutures (74 eyes) for closure of limbal conjunctival incisions in patients undergoing str

75 at the off-label use of fibrin glue to close limbal conjunctival incisions in strabismus surgery resu

76 autograft group and 1 patient (1.0%) in the limbal-conjunctival autograft group developed recurrence

77 ctival autograft group and 105 eyes from the limbal-conjunctival autograft group).

78 junctival autograft transplant (112 eyes) or limbal-conjunctival autograft transplant (112 eyes).

79 Limbal-conjunctival autografts could be a favored option

80 Limbal-conjunctival transplant is safe and more effectiv

81 BM, new BM formed by ex vivo expanded human limbal corneal epithelial cells on iAM deposits much fas

82 for his description of the eponymously named limbal corneal pits as a sign of trachoma.

83 Peripheral and limbal corneal stromal cells (PLCSCs), which contain ker

84 Limbal damage was seen in some eyes with CLAU.

85 Main diagnoses were corneal scar (22.9%), limbal dermoid (21.9%), anterior segment dysgenesis (15.

86 Other associated ophthalmic features were limbal dermoids (2 cases), lateral canthal coloboma (3 c

87 describe the in vivo confocal morphology of limbal dermoids in Goldenhar syndrome and (ii) compare t

88 that IVCM may be a useful technique to study limbal dermoids, given its ability to detect typical mic

89 121 children with VKC, 119 (98.4%) had only limbal disease.

90 Corneoscleral limbal dissection of >/=6 clock hours during wide excisi

91 mean number of clock hours of corneoscleral limbal dissection owing to wide tumor excision (8 vs 7;

92 D in cases requiring extensive corneoscleral limbal dissection.

93 Both corneal and limbal epithelia become progressively thinner in LSCD.

94 ed in all layers of the corneal and anterior limbal epithelia.

95 ls were present in the central and posterior limbal epithelia.

96 rental strain RN6390 to apical human corneal-limbal epithelial (HCLE) cells and to biotinylated cell

97 cytotoxicity in coculture with human corneal limbal epithelial (HCLE) cells, primary human corneal fi

98 the antiadhesive character of human corneal-limbal epithelial (HCLE) cells.

99 A interference in immortalized human corneal-limbal epithelial (HCLE) cells.

100 lium-enriched genes, was associated with the limbal epithelial basal cells.

101 An immortalized human corneal-limbal epithelial cell line (HCLE) expressing the same M

102 An immortalized human corneal limbal epithelial cell line was treated in the presence

103 Human limbal epithelial cells (HLE) and corneal stromal stem c

104 iated corneal epithelial cells, and SSEA4(-) limbal epithelial cells contain a higher proportion of l

105 The limbal epithelial cells of FIH-1 null mice had an increa

106 Primary cultures of pig limbal epithelial cells stained with Hoechst 33342 were

107 ained from a healthy area of the limbus, the limbal epithelial cells were cultured on a denuded human

108 thelial cells and immortalized human corneal limbal epithelial cells were cultured on the SF and denu

109 superior in vitro proliferative potential of limbal epithelial cells, and the transplanted limbal cel

110 nces in glycogen content between corneal and limbal epithelial cells.

111 accounted for an average of 40% of the total limbal epithelial cells.

112 face (OS) epithelial tissue samples from the Limbal Epithelial Crypt (LEC), limbus, cornea and conjun

113 ate angiogenesis, we demonstrated that human limbal epithelial keratinocytes (HLEKs) engineered to ov

114 more mature, resulting in regeneration of a limbal epithelial phenotype.

115 However, the ultimate fate of limbal epithelial progenitor cells in the explant remain

116 During ex vivo expansion on iAM, some limbal epithelial progenitor cells indeed migrate onto i

117 ssessing the success of ex vivo expansion of limbal epithelial progenitor cells on AM.

118 er, on the growth and differentiation of the limbal epithelial progenitor cells when these cells are

119 embrane (iAM) relies on ex vivo expansion of limbal epithelial progenitor cells.

120 ifferentiation and maintain clonal growth of limbal epithelial progenitors.

121 epidermal differentiation without affecting limbal epithelial proliferation.

122 Transplantation of limbal epithelial sheets from explant cultures is one of

123 Destruction of the limbal epithelial stem cell (LESC) population in the cor

124 However, their roles in normal and diseased limbal epithelial stem cells (LESC) remain unknown.

125 Limbal epithelial stem cells repopulate the donor site a

126 Limbal epithelial stem cells were isolated, and cellular

127 was associated with a transient increase of limbal epithelial stem cells.

128 Optimization of culture conditions for human limbal epithelial stem/progenitor cells (LEPC) that inco

129 ional biopsy with and without primary simple limbal epithelial transplantation (p-SLET).

130 -term clinical outcomes of autologous simple limbal epithelial transplantation (SLET), a relatively n

131 treatment (1 eye) underwent modified simple limbal epithelial transplantation at Bascom Palmer Eye I

132 ial LSCD who underwent autologous cultivated limbal epithelial transplantation between 2001 and 2011.

133 Simple limbal epithelial transplantation is probably preferable

134 ement of limbal stem cell deficiency, simple limbal epithelial transplantation seems to be a safe, re

135 Repeat autologous cultivated limbal epithelial transplantation successfully restores

136 Success of cultivated limbal epithelial transplantation, defined as a complete

137 gentle scraping of the surface, leaving the limbal epithelium intact, and epithelium was collected a

138 able on the use of conventional and cultured limbal epithelium using various substrates in the treatm

139 In the present study, T cells in the limbal epithelium were found to predominantly express th

140 abundant, highly enriched transcripts in the limbal epithelium were in general agreement with the SAG

141 laser-capture microdissected populations of limbal epithelium were subjected to real-time quantitati

142 everal genes preferentially expressed in the limbal epithelium with cellular proliferation and migrat

143 IH-1 in vivo increased Notch activity in the limbal epithelium, resulting in a more differentiated ph

144 e mice confirmed that epiregulin, one of the limbal epithelium-enriched genes, was associated with th

145 wed by mechanical debridement of corneal and limbal epithelium.

146 ed in the central and peripheral corneal and limbal epithelium.

147 pact on stem/progenitor cell preservation in limbal explant cultures.

148 imary HCECs were cultured from donor corneal limbal explants and grown to subconfluence.

149 was successfully created by culturing human limbal explants at an air-liquid interface (airlift) for

150 be regenerated in cultures from central and limbal explants of murine cornea.

151 Human limbal explants were cultured on iAM and dAM.

152 Human limbal explants were cultured on iAM for 2 weeks and the

153 Human limbal explants were cultured under airlift with or with

154 Corneal limbal explants were obtained from 2 sites, the harveste

155 human corneal epithelial cells (HCECs) from limbal explants were used in cell culture experiments.

156 eal epithelial cells, established from donor limbal explants, were treated with 11 microbial ligands

157 signs and symptoms, 48% were affected by the limbal form, 33% were affected by the tarsal form, and 1

158 ed pannus formation at the donor site of the limbal graft was noted in 5 eyes (12.8%), with the appea

159 over-the-counter pain medication (P = 0.02), limbal injection (P = 0.03), and increased tear film osm

160 Initial median limbal involvement was 12 clock hours (range, 3 to 12 cl

161 ty, epithelial defect area, conjunctival and limbal involvement, and injury-related complications wer

162 reduced to 759 transcripts specific for the limbal library and 844 transcripts specific for the cent

163 Embryonic chick corneal nerves reach limbal mesenchyme by embryonic day (E)5, encircle the co

164 har Syndrome showed a left, infero-temporal, limbal neoformation, with extension to the left orbital

165 VEGF antibodies and TA blocked IL-6-mediated limbal neovascularization.

166 olySia removal caused defasciculation of the limbal nerve trunk in vivo from E7 to E10.

167 LN) isoforms preferentially expressed in the limbal niche as culture matrices for epithelial tissue e

168 livery of multiple cell types to restore the limbal niche following ocular surface injury or disease.

169 xpression patterns of LN chains in the human limbal niche provided evidence for enrichment of LN-alph

170 Limbal or cornea-specific transcripts were identified th

171 However, whether limbal or corneal vessels are evenly distributed under n

172 creased corneal thickness and degradation of limbal palisade architecture.

173 Normal limbal palisade morphology was absent in aniridia but pr

174 degree of alterations, including loss of the limbal palisades and of the normal epithelial mosaic, cy

175 OCT successfully identified the limbal palisades of Vogt that constitute the corneal epi

176 rneal conjunctivalization and anatomy of the limbal palisades of Vogt.

177 Limbal post-lens tear film gaps were present in 42% of t

178 duction, ocular surface staining, bulbar and limbal redness, tear volume, anterior blepharitis, meibo

179 red the mechanical properties of the complex limbal region effectively.

180 t immunohistochemistry were similar near the limbal region, in the central cornea the subbasal nerve

181 and four Wnt inhibitors were specific to the limbal region, whereas Wnt3, Wnt7a, Wnt7b, and Wnt10a we

182 ted miRNA expression profiles in central and limbal regions of normal and diabetic human corneas.

183 The effect of different limbal regions, enzymatic dissociation methods, and cult

184 through the full thickness of the cornea and limbal regions.

185 s also detected in the clinically unaffected limbal regions.

186 ise capsulotomy formation, clear corneal and limbal relaxing incision construction, lens fragmentatio

187 Refractive surgical technologies such as limbal relaxing incisions, laser-assisted in-situ kerato

188 larly attractive option in those cases where limbal-relaxing incisions are not powerful or predictabl

189 Human limbal rims were acquired and imaged with OCT and confoc

190 Microarray analysis of pig limbal SP cells yielded a molecular signature underscori

191 ithelial stem cells were isolated from human limbal specimens and clonally expanded on a 3T3 feeder l

192 Plk3 in hypoxic stress-induced primary human limbal stem (HLS) and corneal epithelial (HCE) cells, re

193 e system supports enrichment and survival of limbal stem and progenitor cells during the entire culti

194 lls (Muc5AC(-)/CK19(-)) and replenishment of limbal stem cell (DeltaNp63alpha(+)/ABCG2(+)) reserve.

195 clonogenic assay, and expression of putative limbal stem cell (LSC) and corneal epithelial differenti

196 Four eyes of 2 patients developed limbal stem cell compromise confirmed with in vivo confo

197 CESCs is different from human cornea, where limbal stem cell concept has been well established and a

198 We are aware that some new evidence supports limbal stem cell concept in mouse recently.

199 6), corneal keloid (3), chemical injury with limbal stem cell deficiency (2), and dermoid (1).

200 ransplantation (OSST) in patients with total limbal stem cell deficiency (LSCD) owing to various etio

201 Limbal stem cell deficiency (LSCD) was diagnosed clinica

202 using various substrates in the treatment of limbal stem cell deficiency (LSCD), the patient populati

203 Limbal stem cell deficiency (LSCD).

204 iously underwent LAT for unilateral acquired limbal stem cell deficiency after chemical burn.

205 2 years) presenting with clinical suspect of limbal stem cell deficiency and 10 eyes of 10 healthy co

206 If extensive corneal scarring and/or limbal stem cell deficiency are present, techniques such

207 atopathy, medication-related keratopathy, or limbal stem cell deficiency characterized by conjunctiva

208 of age, with clinically diagnosed unilateral limbal stem cell deficiency following ocular surface bur

209 management of soft contact lens wear-related limbal stem cell deficiency in young healthy patients.

210 to 12 clock hours), resulting in a residual limbal stem cell deficiency of 6 clock hours (range, 0 t

211 Severe limbal stem cell deficiency related to CL wear is a clin

212 Database search of patients with severe limbal stem cell deficiency related to CL wear was condu

213 ilateral partial (2 eyes) and total (2 eyes) limbal stem cell deficiency secondary to ocular surface

214 nical success of treating corneas with total limbal stem cell deficiency using limbal biopsy explants

215 Limbal stem cell deficiency was related to advancing age

216 No signs of limbal stem cell deficiency were observed during follow-

217 7 cases, just conjunctival epithelium (total limbal stem cell deficiency) in 5 cases, and mixed epith

218 es, and mixed epithelium in 6 cases (partial limbal stem cell deficiency).

219 r wear, symptoms, location and laterality of limbal stem cell deficiency, coexisting ocular disease,

220 d improves vision in eyes with recurrence of limbal stem cell deficiency, following failed primary su

221 y used surgical techniques for management of limbal stem cell deficiency, simple limbal epithelial tr

222 in the AMT group demonstrated corneal haze, limbal stem cell deficiency, symblepharon, ankyloblephar

223 There was no limbal stem cell deficiency, symblepharon, or diplopia n

224 ful for the noninvasive in vivo diagnosis of limbal stem cell deficiency, with a high degree of conco

225 Some patients also showed signs of limbal stem cell deficiency.

226 is, in the eyes affected by partial or total limbal stem cell deficiency.

227 OMECs) is a promising treatment strategy for limbal stem cell deficiency.

228 ultures is one of the standard treatments of limbal stem cell deficiency.

229 conjunctival mitomycin C injection may cause limbal stem cell deficiency.

230 ence are the most promising for treatment of limbal stem cell deficiency.

231 c keratitis scarring, lipid keratopathy, and limbal stem cell deficiency.

232 atients mimicking interstitial keratitis and limbal stem cell deficiency.

233 ppression for soft contact lens wear-related limbal stem cell deficiency.

234 ing to regulate c-Jun activity, resulting in limbal stem cell differentiation and center epithelial a

235 There was no relationship of limbal stem cell failure with the severity of EEC syndro

236 weeks; corneal opacification, 11%, 4 months; limbal stem cell failure, 8%, 7 months; and corneal vasc

237 ve the evaluation of outcomes after cultured limbal stem cell graft.

238 l deficiency are present, techniques such as limbal stem cell grafting, amniotic membrane transplanta

239 ess in the culture conditions also regulates limbal stem cell growth and fate.

240 e, vascularization, conjunctivalization, and limbal stem cell involvement.

241 thelial cell proliferation, differentiation, limbal stem cell maintenance, and expansion were studied

242 ontribute to the identification of potential limbal stem cell niche factors that are promising target

243 As CHRDL1 is preferentially expressed in the limbal stem cell niche of adult human cornea, we assume

244 de in close proximity in vivo in the corneal limbal stem cell niche.

245 ing (n = 78), symblepharon release (n = 56), limbal stem cell transplantation (n = 26), and lamellar

246 ), conjunctival replacement surgery (COMET), limbal stem cell transplantation and kerotoprostheses.

247 Limbal stem cell transplantation is a viable option for

248 Fourteen eyes (78%) underwent limbal stem cell transplantation with systemic immunosup

249 ve measures may not reverse the disease, and limbal stem cell transplantation with systemic immunosup

250 revealed 9 patients (14 eyes) who underwent limbal stem cell transplantation with systemic immunosup

251 clude bioengineered amniotic membrane graft, limbal stem cell transplantation, conjunctival and extra

252 s probably preferable to other techniques of limbal stem cell transplantation, particularly where cel

253 At final follow-up after limbal stem cell transplantation, there was a stable ocu

254 tation (SLET), a relatively new technique of limbal stem cell transplantation.

255 Limbal stem cells (LSCs) are affected globally and basal

256 Healthy eyes contain a population of limbal stem cells (LSCs) that continuously renew the cor

257 omeostasis and regeneration are sustained by limbal stem cells (LSCs), and LSC deficiency is a major

258 c stress suppresses Plk3 activity to protect limbal stem cells from death and to allow the process of

259 aling are mainly maintained by the activated limbal stem cells originating form limbus, but not from

260 s are consistent with the notion that clonal limbal stem cells randomly activate Krt12 alleles in the

261 althy corneal epithelium showing survival of limbal stem cells was observed in 14 eyes (70%) during c

262 cells (CECs) undergo continuous renewal from limbal stem or progenitor cells (LSCs), and deficiency i

263 of the EEC syndrome include skin defects and limbal stem-cell deficiency.

264 thelial cells contain a higher proportion of limbal stem/progenitor cells.

265 ntact lens sensor (CLS) to measure change in limbal strain associated with placing one side of the fa

266 position was associated with an increase in limbal strain in glaucoma eyes (mean [SE], 44.1 [20.4] m

267 The mean limbal strain increase among patients with glaucoma in F

268 Magnitudes of measured changes in limbal strain were greater in glaucoma eyes with past vi

269 Measured changes in limbal strain were related to estimated changes in IOP a

270 e eye shield prevents sleep position-induced limbal strains during a mean 8-hour sleep period.

271 Furthermore, this study has identified the limbal stroma as yet another MSC niche and presents a ne

272 tion, groups of epithelial cells invaded the limbal stroma of the explants from P1 to P3; p63(+)/panc

273 erved from cells isolated from the remaining limbal stroma when seeded on 3T3 fibroblast feeder layer

274 xplant surface, whereas some also invade the limbal stroma, very likely undergoing epithelial-mesench

275 3(+)/vimentin(+) cells also presented in the limbal stroma.

276 Limbal stromal niche cells expressing SC markers can be

277 Limbal stromal niche cells heterogeneously express embry

278 the organ of Corti, but is detached from the limbal surface.

279 Attempts to screen limbal tissues for suitable implants using molecular ste

280 riented flat-mount corneas together with the limbal tissues were used for immunofluorescence microsco

281 Limbal transplant survival rates at the final follow-up

282 s process does not depend on a breech in the limbal vascular endothelium.

283 for acquiring OCTA images of the cornea and limbal vasculature with substantial consistency.

284 lial corneal opacities and adjacent abnormal limbal vasculature, with or without pseudopterygia in 9

285 exhibit milder phenotypes, such as disrupted limbal vasculature.

286 rats by injecting hypertonic saline into the limbal venous system.

287 rats by injecting hypertonic saline into the limbal venous system.

288 Monoclonal anti-CEP antibody neutralized limbal vessel growth stimulated by CEP-HSA, whereas anti

289 thelial abrasion, platelets localized in the limbal vessels and neutrophils emigrated from the limbal

290 helium induces neutrophil emigration through limbal vessels into the avascular corneal stroma, peakin

291 epithelium that did not directly injure the limbal vessels or the avascular stroma of the cornea.

292 Platelets localize in the limbal vessels throughout the same time course as neutro

293 l vessels and neutrophils emigrated from the limbal vessels to the region of the epithelial wound.

294 Wild-type (WT) leukocytes extravasated from limbal vessels, angiogenic stalks, and growing tip vesse

295 nificantly restored platelet localization in limbal vessels, neutrophil emigration, epithelial cell d

296 ics that extends beyond the limits of normal limbal vessels.

297 bal vitrectomy, and eyes undergoing anterior limbal vitrectomy (P = .543).

298 Two-port anterior limbal vitrectomy was carried out after posterior capsul

299 al posterior capsulorrhexis without anterior limbal vitrectomy, and eyes undergoing anterior limbal v

300 redominately at the periphery of the cornea (limbal zone).