ライフサイエンスコーパス: limited proteolysis

1 gen and for heparin and by susceptibility to limited proteolysis.

2 negatively modulated by proteasome-mediated limited proteolysis.

3 and bell-shaped peak intensity profiles, and limited proteolysis.

4 dant local conformational change detected by limited proteolysis.

5 n folding, thermostability, and tolerance to limited proteolysis.

6 and high structural flexibility as probed by limited proteolysis.

7 truncated protein was slightly diminished by limited proteolysis.

8 nal modifications and regions susceptible to limited proteolysis.

9 rminates at a domain boundary, as defined by limited proteolysis.

10 ses confirmed that HP21 activates proPAP3 by limited proteolysis.

11 ivating procoagulant factors Va and VIIIa by limited proteolysis.

12 ein in the presence and absence of DNA using limited proteolysis.

13 en defined the minimal structured core using limited proteolysis.

14 ntification of a putative domain of xNAP1 by limited proteolysis.

15 g, and enhance the A1 domain's resistance to limited proteolysis.

16 footprinting, fluorescence spectroscopy, and limited proteolysis.

17 ations of Hop and Hop mutants were probed by limited proteolysis.

18 scade by degrading cofactors Va and VIIIa by limited proteolysis.

19 Similar folded regions were detected by limited proteolysis.

20 , P81L and V126D, on the structure of p16 by limited proteolysis.

21 Domain architecture was further studied by limited proteolysis.

22 IEbeta (TFIIEbetac) has been identified by a limited proteolysis.

23 through transmission electron microscopy or limited proteolysis.

24 ts and a structural perturbation revealed by limited proteolysis.

25 r52Pro TTR was generated readily in vitro by limited proteolysis.

26 rsors, or zymogens, that become activated by limited proteolysis.

27 terminal ends of fibronectin, we prepared by limited proteolysis a dimeric 140 kDa (Fib-2)2 fragment

28 ed N-terminal region of IL-1ra is removed by limited proteolysis, a 2-fold increase in the time cours

29 ng of the loop conformation as elucidated by limited proteolysis, accompanied by the impaired binding

30 Limited proteolysis, accomplished by endopeptidases, is

31 actin (Ybt) gene cluster of Yersinia pestis, limited proteolysis afforded a key 11 kDa peptide from t

32 Limited proteolysis also reveals that phosphorylation in

33 Circular dichroism and limited proteolysis analysis indicate that magnesium ind

34 ing nucleotide analogue could be detected by limited proteolysis analysis, a comparison of the stabil

35 Here, through limited proteolysis analysis, we demonstrate that the ev

36 The purified N-terminal domain prepared by limited proteolysis and anion exchange chromatography wa

37 shadowing electron microscopy, combined with limited proteolysis and antibody labeling, demonstrates

38 Limited proteolysis and biochemical experiments indicate

39 Limited proteolysis and chemical cross-linking suggest u

40 Here we used time-resolved limited proteolysis and chemical cross-linking to examin

41 Previous work using limited proteolysis and chemical modification revealed t

42 Limited proteolysis and deletion analyses show that both

43 We have used limited proteolysis and deletion analysis to probe the c

44 Using limited proteolysis and deletion mutagenesis we show tha

45 etence in diabetes; this was confirmed using limited proteolysis and denaturation/refolding.

46 Small-angle X-ray scattering, limited proteolysis and differential scanning fluorimetr

47 However, limited proteolysis and electron microscopy observations

48 ytoplasmic domain of EpsL was identified via limited proteolysis and facilitated the crystallization

49 tting and then examined the mechanisms using limited proteolysis and fluorescence resonance energy tr

50 the intron-maturase complex, and results of limited proteolysis and fluorescence spectroscopy experi

51 Here, we have harnessed the power of limited proteolysis and heterologous protein expression

52 The combination of limited proteolysis and MALDI-TOF mass spectrometry has

53 tion in Ku structure, which was confirmed by limited proteolysis and mass spectrometry analyses.

54 Using limited proteolysis and mass spectrometry, we found that

55 Using limited proteolysis and mass spectrometry, we show that

56 most of the PRLR ECD, a result confirmed by limited proteolysis and mass spectrometry.

57 esonance (SPR) and an approach that combines limited proteolysis and mass spectrometry.

58 Domain mapping of the 98-kDa enzyme by limited proteolysis and NaBH(4) cross-linking with a BER

59 Limited proteolysis and peptide mass mapping of intact p

60 This was confirmed by limited proteolysis and protein sequencing, which showed

61 n partially inhibited, proteasomes carry out limited proteolysis and release the processed Nrf1 (lack

62 kB coiled coil, obtained by a combination of limited proteolysis and site-directed cross-linking appr

63 Limited proteolysis and surface-topology mapping of prot

64 We confirmed these findings using limited proteolysis and tryptophan fluorescence experime

65 have characterized its structure further by limited proteolysis and Western blotting with the use of

66 Each of these toxins undergoes limited proteolysis and/or disulfide bond reduction to f

67 kened zinc binding, increased sensitivity to limited proteolysis, and altered circular dichroism spec

68 tryptophan fluorescence, circular dichroism, limited proteolysis, and cross-linking.

69 erscored by the results of alanine-scanning, limited proteolysis, and deletion analysis, which show t

70 mpetition assays, cross-linking experiments, limited proteolysis, and mass spectrometry revealed that

71 We used immunoblotting, limited proteolysis, and mass spectrometry to localize g

72 In addition, via chemical cross-linking, limited proteolysis, and mass spectrometry, we identifie

73 Infectivity, limited proteolysis, and matrix-assisted laser desorptio

74 Intrinsic fluorescence, limited proteolysis, and molecular dynamics studies sugg

75 imensional nuclear magnetic resonance (NMR), limited proteolysis, and small-angle X-ray scattering (S

76 orbance and circular dichroism spectroscopy, limited proteolysis, and solution NMR.

77 s of CBS indicate that activation by AdoMet, limited proteolysis, and thermal denaturation share a co

78 y Ca(2+), as shown by Cibacron blue binding, limited proteolysis, and tryptophan fluorescence.

79 Intracellular peptides generated by limited proteolysis are likely to function inside and ou

80 We devised an in vitro limited proteolysis assay of Hmg2p in its native membran

81 elevance of this model behavior, we used the limited proteolysis assay to examine the effects of chan

82 nucleotide binding, kinase measurements, and limited proteolysis assays on this semisynthetic ATP-lin

83 We developed limited proteolysis assays using trypsin and V8 protease

84 Limited proteolysis assays using trypsin in the absence

85 Using limited proteolysis assays, nucleotide-binding assays, a

86 determined by UIC2 antibody shift assays and limited proteolysis assays.

87 Limited proteolysis at pH 8.0 shows that the C-terminal

88 polymerization of the large multimers is the limited proteolysis by a vWF-cleaving protease present i

89 istently, DBD-B became more resistant to the limited proteolysis by chymotrypsin after RPA hyperphosp

90 After limited proteolysis by proteinase K, the most abundant f

91 m assumes that FV activation is initiated by limited proteolysis by traces of (meizo) thrombin.

92 and studied changes in Tm susceptibility to limited proteolysis by trypsin and changes in regulation

93 Limited proteolysis by trypsin results in the production

94 Limited proteolysis by trypsin shows that binding of nuc

95 drogen-deuterium exchange mass spectrometry, limited proteolysis, circular dichroism spectroscopy, an

96 Limited proteolysis, circular dichroism, and fluorescenc

97 TRCF mutants are dramatically sensitized to limited proteolysis compared with repressed TRCF, pointi

98 Analysis by limited proteolysis confirmed the identity of Gbeta(5) i

99 Limited proteolysis confirms assembly of stargazin with

100 he isoforms, bRPGRIP1 and bRPGRIP1b, undergo limited proteolysis constitutively in vivo in the cytopl

101 Using limited proteolysis coupled with mass spectrometric anal

102 fied experimentally, with techniques such as limited proteolysis coupled with mass spectrometry, or c

103 ering, high-resolution NMR spectroscopy, and limited proteolysis coupled with mass spectrometry, we s

104 eosome binding ATPase and remodeling assays, limited proteolysis, cross-linking, and tandem mass spec

105 lity of the domain 3-4 linker, inferred from limited proteolysis data and from the relaxation behavio

106 Limited proteolysis data conducted on the MA-CaM complex

107 NMR and limited proteolysis data suggest that approximately 30 a

108 This shape, together with limited proteolysis data, suggests that the S1-RNA bindi

109 Limited proteolysis defined an independently folded C-te

110 Binding of the hydrophobic probe bis-ANS and limited proteolysis demonstrate CII proteins undergo sig

111 Limited proteolysis demonstrates that a structural core

112 as the minimal coiled-coil domain defined by limited proteolysis, did not retain the ability to inter

113 was assayed by biochemical methods including limited proteolysis, dynamic light scattering, CD, and N

114 Limited proteolysis experiments combined with mass spect

115 Cosedimentation and limited proteolysis experiments indicated a similar acti

116 Limited proteolysis experiments reveal redox- and mutati

117 Conversely, limited proteolysis experiments show a reduced protease

118 In addition, limited proteolysis experiments showed a reduced stabili

119 This conclusion is supported by limited proteolysis experiments showing an increase in t

120 Sedimentation velocity and limited proteolysis experiments suggest that heme bindin

121 ns disrupt the STAS domain directly, whereas limited proteolysis experiments suggest that the DeltaY5

122 Limited proteolysis experiments suggest the optimal reac

123 Electrophoretic analysis and limited proteolysis experiments suggested that refolded

124 These observations are confirmed by limited proteolysis experiments that reveal an intrinsic

125 DnaJ using in vitro cross-linking assays and limited proteolysis experiments that show that this inte

126 Also, by systematic truncation and limited proteolysis experiments we identified a portion

127 Limited proteolysis experiments with epitope-tagged copi

128 Circular dichroism and limited proteolysis experiments with F-virosomes indicat

129 ulated ATPase activity and with results from limited proteolysis experiments, which indicated that th

130 In this report, we have used limited proteolysis, filter binding, and NMR spectroscop

131 nsoluble SOD1 amyloid fibrils, we first used limited proteolysis followed by mass spectrometric analy

132 thione S-transferase (GST) pull-down assays, limited proteolysis followed by mass spectrometry, and c

133 62 StAR but were structured as determined by limited proteolysis followed by mass spectrometry.

134 We found limited proteolysis followed by RP-HPLC-MS to be less ti

135 ening, and autoinactivation, consistent with limited proteolysis fragmentation patterns.

136 Limited proteolysis further defines regions of primary s

137 Limited proteolysis, hydrodynamic, and kinetic measureme

138 However, limited proteolysis identifies a sequence flanking the C

139 buted, calcium-dependent enzymes that induce limited proteolysis in a wide range of substrates.

140 te antigens (HLA) bind peptides generated by limited proteolysis in cells and present them at the cel

141 on of GC, fluorescence emission spectra, and limited proteolysis in the absence and presence of Ca2+.

142 Activity is generated following limited proteolysis indicating that the conversion of fa

143 These results suggest that limited proteolysis is another post-translational mechan

144 Cleavage pattern of SMC2 by limited proteolysis is changed upon its binding to ATP o

145 protein fragments in vivo, whereas in vitro limited proteolysis is often employed to probe different

146 we show that a C-terminal domain isolated by limited proteolysis is tetrameric in solution, like the

147 Using circular dichroism spectroscopy, limited proteolysis, kinase assays, and isothermal titra

148 Limited proteolysis, kinetic simulations, and MS analyse

149 We use an array of approaches (limited proteolysis, magic angle spinning NMR, Fourier t

150 Limited proteolysis, mass spectrometry and deletion anal

151 Limited proteolysis, mass spectrometry and N-terminal se

152 nitrificans were supported by cross-linking, limited proteolysis, mass spectrometry, and functional d

153 For this study, we use a combination of limited proteolysis, mass spectrometry, and N-terminal s

154 We used surface plasmon resonance, limited proteolysis, mass spectrometry, and NMR spectros

155 protein to the postfusion conformation using limited proteolysis, mass spectrometry, and single-parti

156 To test this hypothesis, limited proteolysis, mass spectrometry, and solution-sta

157 y from the current model and correlates with limited proteolysis, matrix-assisted laser desorption/io

158 Limited proteolysis measurements confirm the expected do

159 uncated form is generated intracellularly by limited proteolysis mediated by proprotein convertase(s)

160 high-throughput alternative to the classical limited proteolysis method for the isolation of stable c

161 Limited proteolysis, microsequencing, and sedimentation

162 g a combination of top-down and middle-down (limited proteolysis) MS approach, which ensures full seq

163 Limited proteolysis, NMR spectroscopy and chromophoric c

164 s approach, we also performed membrane-based limited proteolysis of a large Arabidopsis GTPase, Root

165 Presenilins have been shown to influence limited proteolysis of amyloid beta protein precursor (A

166 Limited proteolysis of an N-terminal construct from resi

167 Here we test the validity of this model by limited proteolysis of B17 and the characterization of i

168 ells with anti-Fas induced caspase-dependent limited proteolysis of beta-catenin N- and C-terminal re

169 co-regulates the metalloproteinase-mediated limited proteolysis of beta1AR.

170 Limited proteolysis of betaB1 produced betaB1DeltaN47, w

171 Limited proteolysis of BjPutA under reducing conditions

172 Limited proteolysis of BPL with trypsin and chymotrypsin

173 Limited proteolysis of BRCA1/BARD1 complexes, monitored

174 Limited proteolysis of calcineurin in the presence of EG

175 ted by a specific stimulus, caspases execute limited proteolysis of downstream substrates to trigger

176 The structure of the T.acidophilum LplA and limited proteolysis of E.coli LplA together highlighted

177 Limited proteolysis of epitope-tagged c" (Vma16p) indica

178 FV as an inactive procofactor and reveal how limited proteolysis of FV progressively destabilizes key

179 domain in Gis1 and is essential for both the limited proteolysis of Gis1 and its full activity.

180 ied several different aggregating sites from limited proteolysis of harvested aggregates and effects

181 e use for these purposes is the induction of limited proteolysis of host proteins, which is the main

182 We have defined by limited proteolysis of human capping enzyme residues 229

183 Limited proteolysis of IPK1 revealed that N-lobe stabili

184 Limited proteolysis of Lef4 and ensuing COOH-terminal de

185 Employing the method of limited proteolysis of lipid droplet surface proteins, w

186 Limited proteolysis of MAB007 with Lys-C led to a single

187 Limited proteolysis of native and recombinant Cdc37, in

188 solated the N-terminal domain in vitro using limited proteolysis of native OCP.

189 in of approximately 15 kDa was detected upon limited proteolysis of oxidized but not reduced Yap1p.

190 as a protease involved in the degradation or limited proteolysis of parasite proteins involved in inv

191 ere reduced by the PfKelch13 mutation, which limited proteolysis of PfPI3K and thus increased levels

192 Limited proteolysis of PR-B demonstrated that the hydrod

193 uding PC3 (also known as PC1), that catalyze limited proteolysis of precursor proteins.

194 the appearance of a 119-kDa fragment during limited proteolysis of proline-reduced PutA.

195 Limited proteolysis of purified S. aureus DNA ligase by

196 Previously, limited proteolysis of PutA indicated that the conformat

197 einforces the biofilm structure and leads to limited proteolysis of RbmA to RbmA*.

198 Limited proteolysis of RNase-A yields a short N-terminal

199 s, which induce neuronal death by catalyzing limited proteolysis of specific cellular proteins.

200 Limited proteolysis of spheroplasts revealed that the C-

201 a(2+)-activated proteases that result in the limited proteolysis of substrate proteins and subsequent

202 We demonstrated previously that limited proteolysis of talin1 by the calcium-dependent p

203 Limited proteolysis of TbCet1 suggests that the hydrophi

204 competes with FXa for binding to FV(a), and limited proteolysis of the B-domain, specifically at Arg

205 Full or limited proteolysis of the collagen fibril is known to b

206 or the monovalent Fab fragments derived from limited proteolysis of the covalently modified 8A11 were

207 We show that limited proteolysis of the detergent-solubilized and pur

208 Limited proteolysis of the Escherichia coli cell divisio

209 idue domain was identified experimentally by limited proteolysis of the full-length protein and was s

210 Although limited proteolysis of the histone H3 N-terminal tail (H

211 We have used limited proteolysis of the in vitro-translated mouse AhR

212 Limited proteolysis of the NLD revealed characteristic f

213 Limited proteolysis of the pyruvate decarboxylase (E1, a

214 Limited proteolysis of the recombinant A6 protein identi

215 we identified a putative hrg binding site by limited proteolysis of the recombinant extracellular dom

216 Limited proteolysis of the recombinant protein with alph

217 ajor structural transformation, triggered by limited proteolysis of the scaffolding proteins.

218 ent from BPAG1, residues 226-448, defined by limited proteolysis of the whole plakin domain.

219 Circular dichroism spectroscopy and limited proteolysis of these reconstituted particles ind

220 Consistent with this proposal, limited proteolysis of wild-type and hybrid modules show

221 sucrose gradient centrifugation experiments, limited proteolysis, one-dimensional NMR, and beta-lacta

222 rgoing conformational alterations induced by limited proteolysis or elevated temperature.

223 ormed into a potent antiangiogenic factor by limited proteolysis or mild heating.

224 ition to circular dichroism spectroscopy and limited proteolysis, our strategy incorporates nanogold-

225 milar with regard to cellular concentration, limited proteolysis patterns, rate of autophosphorylatio

226 erized the microtubule-binding properties of limited proteolysis products and subcomplexes of DASH, t

227 -ray scattering (SAXS) data-based models and limited proteolysis profiles of some IgG1 mAbs known to

228 Three approaches, ATP hydrolysis assays, limited proteolysis protection, and equilibrium dialysis

229 Finally, limited proteolysis provided direct evidence that the pr

230 Limited proteolysis rapidly degrades MPs to a stable C-t

231 f exposed sites on the fibril as a result of limited proteolysis, regulate these processes and that o

232 d alpha-synuclein very efficiently, and that limited proteolysis resulted in the generation of carbox

233 Experiments on limited proteolysis reveal that a long range conformatio

234 Limited proteolysis revealed that the linker between the

235 Limited proteolysis reveals a bipartite organization con

236 Limited proteolysis reveals that eIF3 binding to the 40S

237 pproach, well-established techniques such as limited proteolysis, reversed-phase (RP) high-performanc

238 Limited proteolysis separates ER from mitochondria, wher

239 Structural analysis and limited proteolysis show that sterol binding closes the

240 Structure probing by limited proteolysis showed that AmEPV ligase is punctuat

241 ourier transformed infrared spectroscopy and limited proteolysis showed that fibrillar PABPN1 has a s

242 Although HspBP1 is encoded by seven exons, limited proteolysis shows that it has only two structura

243 Limited proteolysis, small-angle X-ray scattering, and f

244 Computer-assisted sequence analysis and limited proteolysis studies identified an N-terminal fra

245 Limited proteolysis studies of MutLalpha demonstrated th

246 we used trypsin and chymotrypsin to conduct limited proteolysis studies on synthetic amyloid fibrils

247 Limited proteolysis studies suggest that PR-A asymmetry

248 Limited proteolysis studies suggested that the R445L mut

249 sis on Asp134, and chemical modification and limited proteolysis studies, we propose a catalytic mech

250 mains within core RAG1 were identified using limited proteolysis studies.

251 Sequence comparisons and limited proteolysis suggest that their folds are conserv

252 Limited proteolysis suggested further conformational dif

253 Limited proteolysis supported a structural role for this

254 , but when partially inhibited, they perform limited proteolysis that generates the active form of Nr

255 ay ionisation mass spectrometry (ESI-MS) and limited proteolysis that homocysteine attacks intramolec

256 Using cross-linking and limited proteolysis, the alpha-gamma intersubunit contac

257 DSDO rendered the kinase very susceptible to limited proteolysis; the former preferentially caused th

258 amide peptide using experimental techniques (limited proteolysis, thermal denaturation, urea denatura

259 We have used NMR spectroscopy and limited proteolysis to characterize the structural prope

260 loped a method to produce NS5A and performed limited proteolysis to confirm the domain organization m

261 Here we apply limited proteolysis to cystatin B amyloid fibrils and sh

262 the disease-causing PrP isoform (PrPSc) used limited proteolysis to digest the precursor cellular PrP

263 scence spectroscopy, molecular modeling, and limited proteolysis to examine structural dynamics of th

264 We used NMR spectroscopy together with limited proteolysis to examine the dynamics of the lambd

265 We have used limited proteolysis to generate a fragment of RGS9-1 tha

266 events in the self-assembly of IAPP, we used limited proteolysis to identify an exposed and flexible

267 on NMR spectroscopy, circular dichroism, and limited proteolysis to investigate the effects of these

268 Here, we use limited proteolysis to investigate the role of the N and

269 terium exchange-mass spectrometry (DXMS) and limited proteolysis to probe the folded regions of D-AKA

270 tionally encoded, we used amide exchange and limited proteolysis to probe the structures of these two

271 Here we use limited proteolysis to show that 1) newly synthesized pr

272 We used limited proteolysis to study the organization of the sub

273 st prion protein (PrP), which is assessed by limited proteolysis to yield resistant PrP bands (PrP-re

274 The 88-kDa secreted VacA protein can undergo limited proteolysis to yield two domains, designated p33

275 uctural analyses of tRNase Z(L) performed by limited proteolysis, two dimensional gel electrophoresis

276 Applying limited proteolysis using endoproteinase Lys-C resulted

277 The cysteinylation observed after limited proteolysis was confirmed by peptide mapping cou

278 Limited proteolysis was used to identify a stable and fu

279 Limited proteolysis was used to locate involvement in th

280 Using limited proteolysis we have also shown that HspBP1 assoc

281 Using limited proteolysis, we also identified modifications re

282 Using NMR spectroscopy and limited proteolysis, we demonstrate that several of thes

283 Using in situ limited proteolysis, we demonstrated a clear change in t

284 Here, using limited proteolysis, we map the site of disorder in unli

285 Sequence alignment and limited proteolysis were used to identify and eliminate

286 of dysferlin, we have applied the method of limited proteolysis, which allows nonspecific digestion

287 o interdomain boundaries were identified via limited proteolysis, which led to the production of a 90

288 The p48/p58 dimer was subjected to limited proteolysis, which produced two stable structura

289 e titration, tryptophan emission spectra, or limited proteolysis, which suggests that the E240K mutat

290 The limited proteolysis with Glu-C or Lys-C proteases shows

291 Limited proteolysis with MMP-2 and -9 degraded PEDF in a

292 Limited proteolysis with pepsin was used to determine se

293 Limited proteolysis with trypsin and V8 proteases was us

294 Limited proteolysis with trypsin was employed to demonst

295 lubilized from porcine thyroid microsomes by limited proteolysis with trypsin.

296 dney) or endothelial (human brain) cells, by limited proteolysis with trypsin.

297 mains, with generation of activity requiring limited proteolysis within a region that separates the l

298 Here, we show that RbmA undergoes limited proteolysis within the biofilm.

299 ross-link bond between actin protomers using limited proteolysis, X-ray crystallography, and mass spe

300 type IX collagen (CIX) from cartilage using limited proteolysis yielded partially degraded material.