ライフサイエンスコーパス: limiting dilution

1 -derived clonal populations were obtained by limiting dilution.

2 fusion protein, and the cells were cloned by limiting dilution.

3 urrent methods that rely on washing steps or limiting dilution.

4 ity than WT-infected LCLs for regrowth after limiting dilution.

5 T-cell clones were obtained by limiting dilution.

6 itute secondary lethally irradiated hosts in limiting dilution.

7 ultures, and multiple clones were derived by limiting dilution.

8 s, eight CD4+ T-cell clones were obtained by limiting dilution.

9 l origins by cloning using multiple steps of limiting dilution.

10 and cell lines were recovered by cloning at limiting dilution.

11 feeder layers for cloning lymphoid cells by limiting dilution.

12 d shorter tumor-free intervals in vivo after limiting dilution.

13 cells and clonal isolates were generated by limiting dilution.

14 established 18 single MSC-derived clones by limiting dilution.

15 17(+) CD4(+) T-cell clones were generated by limiting dilution.

16 erformed with clonal populations obtained by limiting dilution.

17 TCC were generated from PBMC by limiting dilution against a mixture of eleven 20-mer ove

18 Limiting dilution analyses (LDA) detected no EBV-specifi

19 By limiting dilution analyses and index sorts, we found tha

20 s of responder cell frequency (RCF) based on limiting dilution analyses are laborious, and alternativ

21 Limiting dilution analyses for IL-2-producing cells and

22 Limiting dilution analyses indicated that an oncogenic K

23 Cell mixing and limiting dilution analyses provide evidence that these c

24 Flow cytometric and limiting dilution analyses suggest that the increase in

25 In this study we describe FACS-based limiting dilution analyses using the dilution of the int

26 Using limiting dilution analyses, the frequency of HSV-specifi

27 In limiting dilution analyses, we found that the Abbot assa

28 r relapse, and LSC frequency was assessed by limiting dilution analyses.

29 cell-mediated lympholysis assays (CML), and limiting dilution analyses.

30 as indicated by Ag-induced proliferation and limiting dilution analyses.

31 ity in transwell assays and clonogenicity in limiting dilution analyses.

32 as determined by in vitro proliferation and limiting dilution analysis (approximately equals 1:190,0

33 protein L6, were equivalent as determined by limiting dilution analysis (approximately equals 1:5,000

34 panded in the presence of IL-2 and tested in limiting dilution analysis (LDA) for reactivity to synth

35 Limiting dilution analysis (LDA) of infiltrating cells r

36 Limiting dilution analysis (LDA) of SIC to determine pre

37 Limiting dilution analysis (LDA) results were also nonli

38 per T-lymphocyte proliferation was tested by limiting dilution analysis (LDA).

39 cine endothelial and lymphoblastoid cells by limiting dilution analysis and analyzed the responding T

40 re PR1-specific CTLs than were identified by limiting dilution analysis and correlated better with ly

41 esponses to all 3 viruses were quantified by limiting dilution analysis and staining with HLA-A*0201

42 te with similarly high cloning efficiency in limiting dilution analysis as CD28(+) CD45RO(hi) cells o

43 Furthermore, in vivo limiting dilution analysis assays revealed that LDN19318

44 ontrols; because of low cell numbers, formal limiting dilution analysis could only be performed in 10

45 Limiting dilution analysis demonstrated a 10-fold increa

46 Limiting dilution analysis demonstrated that proliferati

47 36 mo after transplantation were studied in limiting dilution analysis for allopeptide reactivity.

48 chromium release assay for CTL activity and limiting dilution analysis for CTL precursor frequency,

49 Limiting dilution analysis from these experiments showed

50 Limiting dilution analysis identified an expanded popula

51 Limiting dilution analysis identified one predominant CT

52 Limiting dilution analysis indicated that the frequency

53 Limiting dilution analysis indicated that the T cell pre

54 L responses against different virus strains, limiting dilution analysis is particularly appropriate t

55 ecific CD8+ T cells has been accomplished by limiting dilution analysis of cytotoxic precursor cells.

56 Similarly, as determined by limiting dilution analysis of IL-2-producing cells, T ce

57 Limiting dilution analysis of residual alloantigen-react

58 Limiting dilution analysis of TLR activated, latently in

59 Limiting dilution analysis of transduced Jurkat cells de

60 Limiting dilution analysis of unfractionated blood demon

61 Sequential limiting dilution analysis of virus-specific CD4+ T cell

62 nd multiply spliced viral RNAs combined with limiting dilution analysis provided measurements of tran

63 Similarly, limiting dilution analysis revealed a greater frequency

64 Limiting dilution analysis revealed a high precursor fre

65 Limiting dilution analysis showed a 5- to 6-fold higher

66 Results of limiting dilution analysis suggested that the increase i

67 Limiting dilution analysis suggests that the kidney marr

68 Limiting dilution analysis suggests that these cells can

69 e used monocyte-derived dendritic cells in a limiting dilution analysis to generate Mtb-specific CD8(

70 ral tissues have not been evaluated, we used limiting dilution analysis to measure the concentration

71 d distinguish patients at risk, we performed limiting dilution analysis to quantify anti-EBV cytotoxi

72 equency of BCR-ABL(+) cells as determined by limiting dilution analysis was consistently higher in 38

73 CD1-, and nonclassically restricted cells, a limiting dilution analysis was performed.

74 Limiting dilution analysis was used to assess helper T-l

75 Limiting dilution analysis was used to characterize nume

76 Limiting dilution analysis was used to quantify the loca

77 ells in peripheral blood were quantitated by limiting dilution analysis, and their effect on EBV load

78 Using limiting dilution analysis, CD8(+) T cells isolated duri

79 equencies of 1 in 10(5)-10(6) as measured by limiting dilution analysis, estimates of the T cell freq

80 Using long-term BM cultures and limiting dilution analysis, G0CD34+ cells were found to

81 than, CTL precursor frequencies derived from limiting dilution analysis, indicating that this novel p

82 requencies of helper and cytotoxic cells, by limiting dilution analysis, serially measured in vitro c

83 of keratinocytes into subcutis combined with limiting dilution analysis, to assess the long-term repo

84 Here, by limiting dilution analysis, we anatomically map the deve

85 Using limiting dilution analysis, we demonstrate that the abso

86 Using a limiting dilution analysis, we found the frequency of SR

87 ated cell sorting, cell transplantation, and limiting dilution analysis, we were able to identify the

88 ong term immunologic memory as determined by limiting dilution analysis, with memory T cells displayi

89 Limiting dilution analysis-derived minimal frequency est

90 All recipients had limiting dilution analysis-detectable, graft-reactive cy

91 herwise underestimated with the conventional limiting dilution analysis.

92 e of techniques exploiting the principles of limiting dilution analysis.

93 incorporation of [3H]thymidine into DNA and limiting dilution analysis.

94 xic (CTLf) T cells were enumerated by use of limiting dilution analysis.

95 because few cells score as LCMV specific in limiting dilution analysis.

96 We assessed tumorigenicity using limiting dilution analysis.

97 ), and cytotoxic T cell precursors (pCTL) by limiting dilution analysis.

98 s of the clade B of HIV-1 were determined by limiting dilution analysis.

99 lopeptide reactive T cells was determined by limiting dilution analysis.

100 -lymphocyte precursor (CTLp) frequency using limiting dilution analysis.

101 the HIC and MLR populations, as assessed by limiting dilution analysis.

102 cellular interferon-gamma (IFN-gamma) and by limiting-dilution analysis (LDA) of cytotoxic T-cell pre

103 L reactive to SIV proteins was determined by limiting-dilution analysis and demonstrated that the res

104 Limiting-dilution analysis indicates that Hem-End coloni

105 Limiting-dilution analysis of lymphocytes from animals t

106 M+, IgG+, and RF+ B cells were determined by limiting-dilution analysis of purified peripheral blood

107 l HCMV-specific CTL response, we have used a limiting-dilution analysis system to quantify HCMV-speci

108 antigen-specific memory B cells by means of limiting-dilution analysis, and we compared memory B-cel

109 Using bulk culture analysis and limiting-dilution analysis, we have shown that a replica

110 The DNA material was quantified using limiting-dilution analysis; the mean concentration was d

111 s demonstrated by colony-forming capacity in limiting dilution and by transplantation in Matrigel plu

112 to that of MC progenitors (MCp) measured by limiting dilution and clonal expansion with maturation.

113 ccuracy and sensitivity of this platform for limiting dilution and digital PCR applications.

114 of Rare EpiAlleles by Melt), which uses semi-limiting dilution and precise melt curve analysis to dis

115 lations at a single cell level by cloning at limiting dilution and propagating the resulting CTL clon

116 verified its role as a gastric CSC marker by limiting dilution and serial transplantation assays.

117 Limiting dilution and single-cell experiments show that

118 We demonstrate by limiting dilution and single-cell transplantations that

119 sed seronegative persons were then cloned at limiting dilution and tested for HCV-specific CTL activi

120 levels of cell-surface CD95 were isolated by limiting dilution, and analyzed for sensitivity to CD95-

121 f shorter tumor-free intervals in vivo after limiting dilution, and enhanced migration in invasion as

122 e cells were then restimulated and cloned by limiting dilution, and the clones were analyzed.

123 Using a limiting dilution approach, we found that many individua

124 ed from unseparated bone marrow (BM) through limiting dilution approaches.

125 transplant patients were investigated using limiting dilution assay (LDA) and ELISPOT for interferon

126 NK precursors, NK-LTBMC stroma was used in a limiting dilution assay (LDA) to quantitate the precurso

127 cytokine staining, and precursor frequency (limiting dilution assay [LDA]) cytotoxicity assays.

128 lloreactive cytotoxic T lymphocyte clones by limiting dilution assay at 120 days.

129 D34(+) label-retaining cells, and an in vivo limiting dilution assay for keratinocyte stem cells with

130 We have used a limiting dilution assay of mafosfamide-resistant progeni

131 er transplant.For subject 2, a pretransplant limiting dilution assay revealed T helper cells recogniz

132 Finally, we developed an in vivo limiting dilution assay to compare individual T cell pro

133 Limiting dilution assay was used to calculate the freque

134 bility of HTLV-2 to transform lymphocytes, a limiting dilution assay was used to generate clonal, tra

135 s is particularly relevant in the setting of limiting dilution assay where cells are transplanted in

136 f repopulating cells, as assessed by extreme limiting dilution assay.

137 nfected beta TCR KO mice, as demonstrated by limiting dilution assay.

138 R-incompatible individuals in bulk MLC or in limiting dilution assay.

139 did not initiate or propagate leukemia in a limiting dilution assay.

140 , as demonstrated by both cytotoxicity and a limiting-dilution assay for the estimation of CTL precur

141 particles was assayed for active vector by a limiting-dilution assay to evaluate transduction efficie

142 mphocyte (CTL) and helper T lymphocyte (HTL) limiting dilution assays (LDA) were performed 10 days af

143 Using a variety of techniques, including limiting dilution assays (LDA), intracellular IFNgamma a

144 hypothesis of clonal deletion vs anergy, CTL limiting dilution assays cultures were restimulated with

145 methylcellulose colony-forming units and by limiting dilution assays in vitro.

146 In limiting dilution assays of CD34+Lin- cells, TPO was fou

147 In vivo limiting dilution assays reveal hundreds more precursor

148 Limiting dilution assays revealed that AID expression wa

149 Limiting dilution assays showed a high frequency of anti

150 CD34+ cells cultured in limiting dilution assays showed a sixfold decrease in NK

151 CTL limiting dilution assays showed that CTL precursor frequ

152 Limiting dilution assays suggest the likely mechanism of

153 The Cw3 transfectants were used in limiting dilution assays to estimate the CTL precursor f

154 sented which permits an extended analysis of limiting dilution assays under conditions in which eithe

155 olated peripheral blood mononuclear cells in limiting dilution assays were used to measure HIV-1 Gag-

156 es representing these forms were isolated by limiting dilution assays, and each replicated in cell cu

157 In limiting dilution assays, approximately 25% of unselecte

158 lts in a 10-fold expansion of HSCs in serial limiting dilution assays, indicating that early lymphoid

159 In limiting dilution assays, subcutaneous tumor formation w

160 As determined by limiting dilution assays, the pCTL frequency (p/f) per C

161 + T cells, as detected by IL-2 production in limiting dilution assays, to each of two class II peptid

162 in the number of BCCs, as measured by colony-limiting dilution assays, was observed.

163 Using limiting dilution assays, we examined the CD4+ T cell pr

164 T lymphocytes than traditional proliferative limiting dilution assays.

165 d clonogenicity of LTC-ICs was determined by limiting dilution assays.

166 ed reduction of tumor-initiating capacity in limiting dilution assays.

167 es have been examined only by using in vitro limiting-dilution assays (LDAs).

168 aneous autoreactive T cells, as estimated by limiting-dilution assays.

169 e of growth factors at 200 cells/mL or after limiting dilution at 2 cells/well.

170 resistance selection as well as a regime of limiting dilution at early stages of drug treatment to p

171 ling and computer simulation were applied to limiting-dilution, autologous-transplantation studies in

172 mal cells, sorted cells were transplanted at limiting dilution before and after being cultured for 2

173 ntiviral delivery of MELK-specific shRNA and limiting dilution cell transplantations, we showed that

174 Limiting dilution clones were generated from three bulk

175 Limiting dilution cloning analysis revealed that a major

176 Limiting dilution cloning and analysis of coexpressed TC

177 We used limiting dilution cloning and synthetic peptide Ags to c

178 for 72 h with 5-aza-dC and then subjected to limiting dilution cloning.

179 tb39-specific T cell clones were obtained by limiting dilution cloning.

180 by treatment with 5-aza-2'-deoxycytidine and limiting dilution cloning.

181 rvet subspecies (Chlorocebus pygerythrus) by limiting-dilution coculture, quantitative PCR for viral

182 ing HSCs present in murine bone marrow after limiting dilution competitive transplantation.

183 We used a limiting-dilution competitive repopulation assay with ce

184 one marrow (BM) cells were injected, using a limiting-dilution, competitive transplantation method, i

185 The results of previous limiting-dilution, competitive-repopulation studies in 4

186 , and then examined for tumorigenicity under limiting dilution conditions and clonogenic activity in

187 Under limiting dilution conditions, patients' cells also showe

188 ere cultured on stromal cells and IL-7 under limiting dilution conditions.

189 Limiting dilution CTL assays determined that B6 splenocy

190 The results of a limiting dilution CTL frequency assay were not informati

191 hing T cell clones derived from the joint by limiting dilution culture confirmed coexpression of high

192 ells before and after augmented treatment by limiting-dilution culture of resting CD4+ T cells after

193 omplex class I-derived synthetic peptides in limiting dilution cultures and then compared with contro

194 Limiting dilution data suggest that at least 1 in 65 000

195 Here, we characterized 300 limiting dilution-derived virus isolates from the plasma

196 At limiting dilutions, donor T cell reconstitution of the s

197 otoxic activity as measured in both bulk and limiting dilution effector frequency assays.

198 pervariable region 1 (HVR1), using end-point limiting-dilution (EPLD) technique, from 127 cases invol

199 Limiting dilution ex vivo cultures of resting CD4(+) T c

200 urgdorferi infection and healthy controls in limiting dilution experiments and assessed proliferation

201 Limiting dilution experiments using MPeMSCs and endothel

202 here and holoclone assays as well as in vivo limiting dilution experiments, in 13 patient-derived sam

203 Limiting-dilution experiments demonstrated that the freq

204 Limiting-dilution experiments demonstrated that the limi

205 and HIV-1 IIIB were serially measured using limiting dilution followed by in vitro stimulation with

206 erformed by laser-capture microdissection or limiting dilution, followed by careful exponential cDNA

207 HIV-1-specific CTL lines were derived by limiting dilution following Ag-specific stimulation of P

208 we generated 346 short term T cell lines at limiting dilutions from six normal individuals to tetanu

209 ic T cells will proliferate extensively at a limiting dilution in response to infections.

210 A limiting dilution in vivo transplantation assay was used

211 CTL frequency was measured by limiting dilutions in five patients.

212 easure of infectious HIV-1 titers involves a limiting dilution-infection assay and a calculation of t

213 3 patients, who received T cells produced by limiting dilution methods, but persisted 5 to 9 weeks in

214 t HSC expansion in culture, as determined by limiting dilution mouse reconstitution analyses.

215 hese separate populations were quantified by limiting-dilution nested polymerase chain reaction.

216 Digital PCR (dPCR) exploits limiting dilution of a template into an array of PCR rea

217 lones isolated from 4 vaccinated patients by limiting dilution of bulk tumor-reactive T cells solely

218 fusivities approximately 10(-3) m(2) s(-1)), limiting dilution of FIB and other runoff-associated pol

219 The pattern of cytokines produced from limiting dilution of naive T cells demonstrated that the

220 ) offers absolute quantification through the limiting dilution of template nucleic acid molecules and

221 m xenograft tumors efficiently in vivo using limiting dilutions of cells.

222 Limiting dilutions of CMV-seropositive or -seronegative

223 d from seminiferous tubules, were mixed with limiting dilutions of dispersed mammary epithelial cells

224 s (including the NK precursors), seeded with limiting dilutions of the cells to be quantitated, cultu

225 ecific T cells in the expanded population by limiting dilution or by using fluorescently labeled tetr

226 rowell array platform, combining sampling by limiting dilution or FACS, with imaging and high through

227 munized with spores and were cloned twice by limiting dilution or growth on semisolid medium.

228 ized RBCs or parasite cloning is achieved by limiting dilution or micromanipulation.

229 Limiting-dilution orthotopic xenografts of these BCSCs r

230 es were compared to sequences recovered from limiting-dilution outgrowth assays of resting CD4(+) T c

231 f cells reactivating gammaHV68 in vitro) and limiting dilution PCR (frequency of cells carrying gamma

232 HIV was detected and quantified by limiting dilution PCR and genetically characterized in t

233 Limiting dilution PCR has become an increasingly useful

234 We also describe a discriminatory limiting dilution PCR method that can be used to optimiz

235 mice carry latent gammaHV68, we developed a limiting-dilution PCR assay to quantitate the frequency

236 liminary study of 7 lung recipients, we used limiting-dilution PCR to quantify peripheral blood micro

237 qualitative-, quantitative-, real-time- and limiting dilution-PCR methods, are discussed.

238 heavy chain variable region patient-specific limiting-dilution polymerase chain reaction (PCR).

239 Limiting dilution precursor frequency assays demonstrate

240 r than that for B6 anti-BALB/c determined by limiting dilution proliferation assays.

241 n techniques (NAATs) in digital format using limiting dilution provides potential advantages that hav

242 n- and mixed-phase hybridization formats and limiting dilution quantitative PCR.

243 fied resting memory cell subpopulations by a limiting-dilution, quantitative viral outgrowth assay (Q

244 We used limiting dilution reactivation (frequency of cells react

245 Limiting dilution reconstitution of lethally irradiated

246 bent spot-forming cell (ELISPOT) assay and a limiting dilution responder cell frequency (RCF) assay.

247 population of B. burgdorferi, and cloning by limiting dilution resulted in the identification of two

248 mployed array-based transcript profiling and limiting-dilution reverse transcription-PCR (RT-PCR) met

249 Limiting-dilution reverse transcription-PCR confirmed th

250 Limiting-dilution reverse-transcribed PCRs confirmed dow

251 enus-specific 16S rRNA was quantified with a limiting dilution RT-PCR assay.

252 po(-/-) recipients or controls, we performed limiting-dilution secondary transplants using donor cell

253 transduced with a lacZ retrovirus, cloned by limiting dilution, seeded onto a physiologic dermal subs

254 present in <3% of genomes were confirmed by limiting dilution sequencing.

255 nalysis of CTL precursor (pCTL) frequency by limiting dilution showed that the frequency of pCTL to t

256 Results from limiting dilution studies and cell-mixing experiments su

257 Limiting dilution studies indicated that mutations prese

258 Limiting dilution studies with cell cultures derived fro

259 Limiting-dilution subisolates (LDSIs) of each strain wer

260 nes, and cloned tumor cells were obtained by limiting dilution techniques.

261 not require external calibration, end-point limiting dilution technologies, collectively termed "dig

262 At limiting dilutions, the d-AQuA reactions follow a stocha

263 By limiting dilution, there is a tight correlation between

264 in patients with or without CP by end-point-limiting dilution titer.

265 blood from each flow-through was measured by limiting dilution titration.

266 ions enriched from coelomocytes were used in limiting dilutions to obtain samples of single cells tha

267 imaging and their rarity when measured with limiting dilution transplant.

268 Limiting dilution transplantation and competitive repopu

269 human breast cancer, we have identified, by limiting dilution transplantation and in vitro mammosphe

270 Using a limiting dilution transplantation assay in immunocomprom

271 Two recent studies use fate-mapping and limiting dilution transplantation assays to identify SRY

272 Limiting dilution transplantation assays were performed

273 Leukemia onset was delayed, and limiting dilution transplantation experiments demonstrat

274 y stem/progenitor cell activity as judged by limiting dilution transplantation experiments of primary

275 Using limiting dilution transplantation performed on p53 null

276 Finally, limiting dilution transplantation revealed that p53 null

277 Single cell limiting dilution transplantation with bone marrow from

278 In limiting dilution transplantation, they proliferated to

279 Using in situ genetic lineage tracing and limiting dilution transplantation, we have unravelled th

280 R-1 in PAR-1-deficient cells combined with a limiting-dilution transplantation assay demonstrated the

281 However, limiting-dilution transplantation showed a decrease in t

282 Results from limiting dilution transplantations indicate higher frequ

283 Limiting dilution transplants further demonstrated that

284 n, as assayed by immunophenotypic markers or limiting dilution transplants, we observed increases in

285 s exhibit little to no stem cell activity in limiting dilution transplants.

286 We performed limiting dilution, transwell, and cell viability assays

287 oid-specific B cells by culturing B cells in limiting dilution upon irradiated CD40L-expressing EL4.B

288 n in a standard culture assay, as well as by limiting dilution using multiple short-term T-cell lines

289 Limiting dilution was shown to be essential for correct

290 A T cell clone derived from this line by limiting dilution was shown to be pathogenic when the sa

291 Ten clones obtained by limiting dilution were all CD4+ and of a T-helper-1-like

292 roximately 180 viral genomes were present in limiting dilutions where as few as 16 cells were examine

293 E/nestin:EGFP-sorted cells were multipotent: limiting dilution with clonal expansion as neurospheres,

294 timulation of T cells from IL-7R -/- mice at limiting dilution with immobilized Abs to CD3, CD4 or CD

295 Progenitors were cultured in limiting dilutions with interleukin-7 (IL-7), flt3 ligan

296 precursor cells were defined by competitive limiting dilution without enrichment, tissue culture, or

297 by CD1d1-reactive T cell clones isolated by limiting dilution without preselection for NK1 expressio