ライフサイエンスコーパス: linear plasmid

1 bha128, a 453-nucleotide gene of the 175-kb linear plasmid.

2 rised a two-gene operon located on the 49-kb linear plasmid.

3 ith B. burgdorferi N40 DNA on a single 48-kb linear plasmid.

4 the 9.0-kb circular plasmid and the 27.5-kb linear plasmid.

5 B. hermsii had a 180-kb circular instead of linear plasmid.

6 of Escherichia coli known to lysogenize as a linear plasmid.

7 rmed 'pJAZZ' vector) that is maintained as a linear plasmid.

8 t Borrelia burgdorferi is located in a 54-kb linear plasmid.

9 BHA007 protein, which is encoded by a 174-kb linear plasmid.

10 This new element was demonstrated to be a linear plasmid.

11 d either a 90-kb (pCSL1) or a 140-kb (pCSL2) linear plasmid.

12 cent DNA rearrangement among a number of the linear plasmids.

13 ceeds much faster with supercoiled than with linear plasmids.

14 subfamilies were sequenced and localized to linear plasmids.

15 ns, including a 950-kb linear chromosome and linear plasmids.

16 mosome and a number of variable circular and linear plasmids.

17 n of circular subgenomes with CMS-S-specific linear plasmids.

18 rgdorferi has over 20 different circular and linear plasmids.

19 linear plasmid (lp25), and either the 28-kb linear plasmid 1 or 4 (lp28-1 and lp28-4, respectively)

20 Strains missing linear plasmid 25 (lp25(-)) are able to infect mice if t

21 dification (R-M) systems, BBE02 contained on linear plasmid 25 (lp25) and BBQ67 contained on lp56, ha

22 tick vector and mammalian host both require linear plasmid 25 (lp25).

23 Linear plasmid 25, which is often lost in laboratory str

24 rated a deletion mutant designated ES10 in a linear plasmid 25-negative isolate of B. burgdorferi str

25 nactivation and genetic complementation of a linear plasmid-25-encoded gene (bbe16) to assess its rol

26 B. burgdorferi spirochetes that lack the linear plasmid 28-1 (lp28-1) succumb to the host's immun

27 of putative tick-specific determinants (e.g. linear plasmid 28-4 genes).

28 B. burgdorferi linear plasmid 38 (lp38) contains several genes that are

29 onger infectious and lacked genes encoded on linear plasmids 38 and 28-1, among other differences.

30 revealed significant upregulation of several linear plasmid 54 (lp54)-encoded open reading frames (OR

31 mid-encoded genes of B. burgdorferi, several linear plasmid 54 (lp54)-encoded open reading frames (OR

32 the core Borrelia genome (linear chromosome, linear plasmid 54 and circular plasmid 26) that encode f

33 ates numerous genes encoding lipoproteins on linear plasmid 54 in response to environmental cues.

34 rupt CBP1 in H. capsulatum using a telomeric linear plasmid and a two-step genetic selection.

35 a mutant of 297 (M297) that lacks the 49-kb linear plasmid and expression of outer surface proteins

36 evealed that the gene was located on a 53-kb linear plasmid and that there was only one locus for the

37 Bidirectional replication of Streptomyces linear plasmids and chromosomes from a central origin pr

38 Linear plasmids and chromosomes of the bacterial genus S

39 By modification of palindrome-generating linear plasmids and development of a novel procedure tha

40 locations within telomeres of streptomycete linear plasmids and investigated mechanisms that enable

41 Streptomyces linear plasmids and linear chromosomes can replicate als

42 zed and contains a linear chromosome, twelve linear plasmids and nine circular extra-chromosomal elem

43 d with cells that lost the 24.7- and 27.5-kb linear plasmids and the 9.0-kb circular plasmid.

44 exchange of genetic information between the linear plasmids and the right end of the linear chromoso

45 ent Borrelia hermsii, located it in a 180-kb linear plasmid, and demonstrated its expression.

46 n B31 MI consists of a linear chromosome, 12 linear plasmids, and 9 circular plasmids.

47 Fourteen isolates harbored all B31-like linear plasmids, and seven isolates simultaneously lacke

48 Here we report that long-palindromic linear plasmids are formed in Streptomyces following dou

49 a burgdorferi contains abundant circular and linear plasmids, but the mechanism of replication of the

50 conversion of a unique expression site on a linear plasmid by an archived variable antigen gene.

51 able to repair transformed dephosphorylated linear plasmids by non-homologous end joining with consi

52 ilar but not identical to those reported for linear plasmids carried by these organisms.

53 richia coli accumulate high-molecular-weight linear plasmid concatemers when transformed with plasmid

54 . rochei chromosome and to 100-kb and 206-kb linear plasmids contained in S. rochei, were isolated an

55 e recombinant loci generated by insertion of linear plasmids containing either base-pair substitution

56 s serve as templates for synthesis of duplex linear plasmids containing long palindromes.

57 e rlrA/rorA locus of pSLA2 were seen also on linear plasmids derived from the unrelated SLP2 replicon

58 r Genomic Research) maps to one of the 28-kb linear plasmids (designated lp28-4) that is not present

59 he maternal (stromal) parent possessed three linear plasmids, designated Callan-a (7.5 kb), Aubonne-a

60 The loss of the 27.5- and 40-kb linear plasmids did not decrease the infectivity of thes

61 in vitro patterns of KMnO(4) modification in linear plasmid differed from that in supercoiled plasmid

62 ed isolated supercoiled, relaxed-circular or linear plasmid DNA and isolated mtDNA.

63 a, or ku80delta hdf1delta strains: repair of linear plasmid DNA and repair of an HO endonuclease-indu

64 The assay employs linear plasmid DNA containing DNA DSBs produced by the r

65 tive within the procapsid and recircularizes linear plasmid DNA containing two terminal loxP recognit

66 The ability of each homologue to cross-link linear plasmid DNA has been determined, with a rank orde

67 products suggests that high-molecular-weight linear plasmid DNA is a substrate for RecBCD-mediated re

68 dimer forms stable complexes at both ends of linear plasmid DNA that protect the DSBs from digestion

69 clease-null AdnAB is a helicase that unwinds linear plasmid DNA without degrading the displaced singl

70 propyl)carbodiimide (EDC) in the presence of linear plasmid DNA.

71 d media was also transformed by pure CCC and linear plasmid DNA.

72 ization of cohesive and blunt-end terminated linear plasmid DNAs following transformation.

73 lution and the mobilities of circular versus linear plasmid DNAs were also affected by the chemical f

74 ent and genomic diversity among isolates are linear plasmid driven.

75 inding protein (SSB) in reactions containing linear plasmid dsDNA allowed us to study the AdnAB helic

76 A DNA library of pRJ28, a large linear plasmid encoding mercury resistance, was construc

77 ccomplish replication of telomere-containing linear plasmids, expression of rlrA, which encodes two L

78 frequent occurrence of ectopic integration, linear plasmid formation, and spontaneous resistance to

79 t least two successive rounds of addition of linear plasmid genetic material to the chromosomal right

80 e near the right telomere are often found on linear plasmids in B. burgdorferi (sensu stricto) isolat

81 and circular forms in B. turicatae but only linear plasmids in B. parkeri, which should be of intere

82 spirochetes and is carried on variably sized linear plasmids in both Borrelia parkeri and B. turicata

83 some approximately 1 Mb in size and multiple linear plasmids in the 16- to 200-kb size range.

84 ently renamed mlp, was found on circular and linear plasmids in the genome sequence of B. burgdorferi

85 d-derived DNA probes used were also found on linear plasmids in the related Eurasian Lyme agents Borr

86 vspB of B. turicatae were near the centre of linear plasmids instead of near the telomeres.

87 lar to protelomerase and parA genes found in linear plasmid-like phages with telomeric ends.

88 protein paralogs from distinct circular- and linear-plasmid loci.

89 Some plasmids, including the linear plasmid lp17, are never or rarely reported to be

90 a noncoding region of the 200-kb B. hermsii linear plasmid lp200.

91 s that possess the gene carry it on a 200-kb linear plasmid (lp200), whereas isolates that lack the g

92 mosomal telomere with a large portion of the linear plasmid lp21, which is present in the strain B31

93 The 25-kb linear plasmid lp25 and one of the 28-kb linear plasmids

94 e of replacing the requirement for the 25 kb linear plasmid lp25 during mammalian infection.

95 n-modification gene product expressed by the linear plasmid lp25.

96 Two vectors derived from linear plasmids lp25 and lp28-1 were constructed and sho

97 tal data suggest that two of these elements, linear plasmids lp25 and lp28-1, play essential roles fo

98 The presence of the linear plasmids lp25 and lp56 of Borrelia burgdorferi B3

99 y the 9-kb circular plasmid (cp9), the 25-kb linear plasmid (lp25), and either the 28-kb linear plasm

100 vlsE, a gene localized near one end of linear plasmid lp28-1 and encoding a surface-exposed lip

101 The loss of linear plasmid lp28-1, which contains the vls antigenic

102 -kb linear plasmid lp25 and one of the 28-kb linear plasmids (lp28-1) are required for experimental i

103 aracterize the role of one of the four 28-kb linear plasmids, lp28-3, we generated strains specifical

104 To investigate the role of the 36 kb linear plasmid, lp36 (also designated the B. burgdorferi

105 The conserved linear plasmid lp54 encodes several proteins important f

106 Linear plasmid lp54 is one of the most highly conserved

107 The absence of the linear plasmid lp56, which carries another putative rest

108 tion, but the genetic elements necessary for linear plasmid maintenance have not been experimentally

109 er chromosomes by transient integration, but linear plasmids may lead the donor chromosome end-first

110 Our observations suggest that Streptomyces linear plasmids may occupy an evolutionarily intermediat

111 d encodes, in its chromosome and each of the linear plasmids, members of the lambda exonuclease famil

112 These vectors identify internal regions of linear plasmids necessary for autonomous replication in

113 hbA is a single genetic locus that maps to a linear plasmid of approximately 220 kb.

114 A 28 kb linear plasmid of B. burgdorferi B31 (lp28-1) was found

115 y, we demonstrate that a factor encoded on a linear plasmid of B. burgdorferi, lp17, can negatively r

116 lp6.6 gene, which was localized to the 49-kb linear plasmid of B. burgdorferi, subsequently was clone

117 A linear plasmid of Borrelia burgdorferi had 16,927 bp, a

118 pSCL, a 12 kb linear plasmid of Streptomyces clavuligerus, contains, n

119 ied protein attached to the end of the pSLA2 linear plasmid of Streptomyces rochei, determined the N-

120 covalently bound to protein, pSLA2, a 17 kb linear plasmid of Streptomyces rochei, initiates replica

121 Atypically large linear plasmids of 92 to 105 kb have been identified in

122 and little characterized to date, are large linear plasmids of approximately 160 kb, or approximatel

123 enes were most similar to those of the 54-kb linear plasmids of LD species.

124 Multiple circular and linear plasmids of Lyme disease and relapsing fever Borr

125 ethods, we determined the sequences of large linear plasmids of two New World species: Borrelia herms

126 sequence of the 51,601-bp Klebsiella oxytoca linear plasmid pKO2, and we demonstrate experimentally t

127 pVAPN is similar to the linear plasmid pNSL1 from Rhodococcus sp. NS1 and harbor

128 lasmids with sequence similarities to the 12 linear plasmids present in the B. burgdorferi type strai

129 The linear plasmid profile of CA434 was similar to that of C

130 ng an evolutionary heritage from an N15-like linear plasmid prophage ancestor.

131 The linear plasmid prophage form of PhiHAP-1 begins with the

132 only phages N15 and PY54 are known to have a linear plasmid prophage with closed hairpin telomeres.

133 The 17-kb linear plasmid pSLA2 has been a useful model in studies

134 The Streptomyces linear plasmid pSLA2 initiates DNA replication bidirecti

135 all B. burgdorferi clinical isolates contain linear plasmids related to each other, but the structure

136 n analyzed biochemically, and their roles in linear plasmid repair in vivo have been verified genetic

137 NHEJ can religate transformed linear plasmids, repair ionizing radiation (IR)-induced

138 alyses provide insight into the mechanism of linear plasmid replication and the mechanisms by which p

139 parently not required for either circular or linear plasmid replication.

140 gene cluster, which is located on the 356-kb linear plasmid SCP1 of Streptomyces coelicolor A3(2).

141 ermination of the 365,023 bp sequence of the linear plasmid SCP1.

142 The majority of linear plasmids showed variation both in terms of presen

143 ion of infectivity with strains possessing a linear plasmid (size range, 24 to 36 kb) that hybridized

144 ded Orfs encoded by several ORFs of the lp36 linear plasmid, such as BBK07 and BBK19, and proteins of

145 Linear plasmid templates for RNA polymerases (Pols) I an

146 a suggest that Polintons have evolved from a linear plasmid that acquired a retroviral integrase at l

147 Here we identify 11 genes localizing to linear plasmids that are up-regulated at pH 7.0 relative

148 Notably, they harbour large linear plasmids that contribute to their catabolic diver

149 ing; and (iii) formation of long palindromic linear plasmids that duplicate the intact telomere by a

150 relia carry a linear chromosome and numerous linear plasmids that have covalently closed hairpin telo

151 vspA and vspB were each located on different linear plasmids that were the same in both serotypes.

152 Although derived from linear plasmids, the vectors are maintained in circular

153 Rather, the loss of the 27.5-kb linear plasmid was associated with a more disseminated i

154 A 3.0-kb HindIII fragment of the 24.7-kb linear plasmid was used as a probe to determine the pres

155 The central approximately 25 kb of all 4 linear plasmids was syntenic for orthologous genes for p

156 Linear plasmids were transformed into Escherichia coli s

157 This was located on one linear plasmid, which was defined by the upstream sequen

158 Linear plasmids with noncompatible ends introduced to We

159 dorferi (sensu stricto), for the presence of linear plasmids with sequence similarities to the 12 lin

160 p subfamily of alleles, which are carried on linear plasmids within each cell and maintained in sever