ライフサイエンスコーパス: linearize

1 rO) bonds and the trans U-O-C(ipso) angle is linearized (157.67 degrees versus 147.85 degrees and 130

2 A (MA) but not mitomycin C (MC) cross-linked linearized (32)P-pBR322 DNA in the presence of dithiothr

3 and architecture in spiking networks rely on linearizing activity around a central operating point an

4 The linearized Ad DNAs for active viral replication polyplex

5 A fully linearized analog, in which all six cysteines were repla

6 Smc2/4 binds both linearized and circular plasmids, and the binding appear

7 This vector is then linearized and introduced into a recombination-proficien

8 This modification results in attachment of a linearized and monoglycosylated derivative of enterobact

9 model library, the cyclic depsipeptides are linearized and released from the solid support to the li

10 inserting an ermAM cassette, and then it was linearized and transformed into S. gordonii DL1 for alle

11 Spectroscopic evidence for such linearized and twisted structures was found in the pigme

12 y diseases were 99.0%, 97.1%, and 84.2% with linearized annual recurrent regurgitation of 0.63%, 2.19

13 lymer dynamics inherently nonlinear, and the linearizing approximations required for the normal-mode

14 tion, indicating the recognition of a longer linearized arabinan epitope.

15 ics with rotational degrees of freedom, once linearized around a non-equilibrium steady state charact

16 In this study, the linearized arterial stress-strain relationship is implem

17 A is terminated when the transferred strand, linearized at the 38 base-pair origin of transfer (oriT)

18 Each of these was linearized at unique sites in the vectors and YAC arms w

19 ed utilizing the all electron full-potential linearized augmented plane wave (FLAPW) approach across

20 lculations performed with the full-potential linearized augmented plane wave (FLAPW) method show that

21 me), a nonlinear model had to be used, and a linearized biexponential (LinBiExp) model proved very ad

22 e chromosome of A. actinomycetemcomitans was linearized by digestion with a restriction endonuclease

23 The vector is then linearized by digestion with nicking and restriction end

24 ith either one or two restriction sites were linearized by EcoR124I at similar rates, although the tw

25 53mer uracil-containing scaffold to M13mp7L2 linearized by EcoRI.

26 sented on a 21-in. computer display suitably linearized by gamma correction.

27 This plasmid is linearized by in vivo expression of the meganuclease I-S

28 or in the absence of substrates but could be linearized by preincubating the enzyme with inhibitor, a

29 ids but does bind to plasmids that have been linearized by treatment with ionizing radiation.

30 ithmic encoding mechanisms, which are later "linearized" by education.

31 Phosphodiesterases (PDE-As) end signaling by linearizing c-di-GMP to 5'-phosphoguanylyl-(3',5')-guano

32 Linearized, centromere-containing plasmid DNA with the 5

33 of the serial assembly was first achieved by linearizing circular plasmid dsDNA using BamH I endonucl

34 or mismatch site, the nicked templates were linearized close to either site and assayed.

35 o cleave an internal phosphodiester bond and linearize concatemers during rolling circle replication.

36 Recently, a novel linearized constitutive model with a new strain measure

37 AR cloning vector was constructed that, when linearized, contained a small amount (381 bp) of 3' hypo

38 This phosphodiesterase linearizes cyclic bis(3'-->5')diguanylic acid, an allost

39 blished by lipofection and microinjection of linearized cytomegalovirus (CMV)-CTCF construct into fer

40 production (Delta VE/Delta VCO(2)), and the linearized Delta tidal volume/Delta VE (Delta VT/Delta l

41 sible for attachment of a C-glycosylated and linearized derivative of enterobactin, an iron scavenger

42 dition strongly inhibited the effects of its linearized, disulfide-free variant, [A6, A8, A13, A15]pr

43 Linearized DNA and plasmids containing Histoplasma telom

44 Transcription of genes introduced as linearized DNA can be serially assayed for weeks in each

45 gene copies were made based on circular and linearized DNA conformations using two genomes from each

46 Thus, the end of a linearized DNA fragment can initiate new DNA synthesis b

47 r, we had found that in wild-type (WT) cells linearized DNA is degraded to completion by RecBCD nucle

48 rejoined molecules revealed that >95% of the linearized DNA were precisely end-joined.

49 In the absence of ions, cyclophilins bind linearized DNA.

50 at cells compared to PC12 cells in rejoining linearized DNA.

51 ) DNA and can separate single-strand nicked, linearized (double-strand nicked), fully relaxed, partia

52 CeP3W cleaves linearized, duplex DNA as well as supercoiled plasmid.

53 ed this approach to generate accelerated and linearized dynamics, sinusoidal oscillations with desire

54 V. cholerae, a bacterium that utilizes linearized Ent, can also be weakly targeted.

55 d in detail with full mathematical rigor for linearized equatorial primitive equations with source te

56 tide (Ku70) to enhance cellular uptake and a linearized expression vector to induce enhanced expressi

57 Fragments are then combined with linearized expression vectors, assembled in vitro as par

58 l (1D) continuous wavelet transform (CWT) of linearized fluorescence resonance energy transfer (FRET)

59 Minute virus of mice (MVM) replicates via a linearized form of rolling-circle replication in which t

60 ed using known concentrations of D-Lac and a linearized form of the equilibrium expression.

61 larized DNA molecule than from the identical linearized form.

62 t models for the ion distribution, exact and linearized forms of the planar Poisson-Boltzmann equatio

63 ating in vacuum is derived directly from the linearized general relativistic wave equation alone, for

64 Linearized generalized estimating equation analyses rela

65 tion, since in vitro production of mRNA from linearized GFP plasmid was also blocked by caging and su

66 The log TOF-eta relationship can often be linearized, giving rise to a Tafel law, which allows the

67 Numerical simulation with the full linearized Hamiltonian shows that significant amount of

68 force microscopy-based analysis that uses a linearized-Hertz method to measure individual mechanical

69 We have artificially introduced linearized HPV18 genomic DNA into primary keratinocytes

70 rectification in symmetrical solutions, are linearized in nonsymmetrical solutions that bring the K+

71 h in which their path integral expression is linearized in the difference between forward and backwar

72 0 CTG.CAG repeats or 43 CGG.CCG repeats were linearized in vitro near the center of the repeats and w

73 A linearized Langmuir adsorption isotherm was used to obta

74 ternative conformations, branched (BMH) and 'linearized' (LDI) and switch between them to achieve dif

75 Eigendecomposition of the linearized mean-field contact network model reveals func

76 ghted nonlinear regression (WNLR) and a fast linearizing method known as Patlak analysis.

77 lternative to Patlak analysis and other fast linearizing methods for parametric imaging.

78 To test this hypothesis, we injected a linearized, methylated, and partially single-stranded re

79 The PCR method specifically amplified whole linearized minicircle kinetoplast DNA (kDNA) of the Leis

80 lysis of the characteristic equation for the linearized model demonstrates the existence of a Hopf bi

81 A linearized model for small strains is also obtained as v

82 the variable selection method in the locally linearized model in CONTIN, were examined using the five

83 A linearized model is also re-analysed to calculate the ho

84 The linearized mortality rate was 3.1%/patient-year (porcine

85 inds competitively to a segment of mRNA of a linearized mRNP, passing through the NPC on its way to t

86 y higher relaxivities than the corresponding linearized multimers (Gd(2)C2: r(1) = 10.5 mM(-1) s(-1)

87 ted to evaluate the influence of selectively linearizing one or the other supercoiled domains created

88 n of performance for these stellarators than linearized or local theories that suffer from a failure

89 e determined for fast nonlinear diffusion by linearizing Otto's gradient descent model at the Barenbl

90 Here we show the efficient linking of linearized PACs containing alpha satellite DNA from chro

91 cins 6 and 13 efficiently cross-linked EcoRI-linearized pBR322 DNA upon addition of Et3P.

92 ross-linking was detected in the reaction of linearized pBR322 DNA with 1,12-diaziridinyl-4,9-diazado

93 e continuous presence of ZD 7288, reversibly linearized peak V-I and instantaneous I-V relationships

94 calculations combined with exact solution of linearized phonon Boltzmann equation.

95 OHC somatic electromotility is modeled by linearized piezoelectric relations whereas the OHC hair-

96 LacI with a high affinity (Osym) operator on linearized plasmid (2500 bp) DNA over a wide range of ma

97 recombination events were observed when the linearized plasmid carried short terminal regions homolo

98 mpared it with expression from a transfected linearized plasmid containing an integrated provirus.

99 nal control fragment recovered from the 125I linearized plasmid did not exhibit endo IV sensitivity i

100 of linear DNA in Escherichia coli cells, we linearized plasmid DNA at a specific site in vivo and mo

101 odel end joining of restriction endonuclease linearized plasmid DNA in whole cell extracts.

102 The simultaneous delivery of siRNA and linearized plasmid DNA on the surface of a single nanocr

103 One requires NHEJ of linearized plasmid DNA transformed into the test organis

104 ar recombinogenic recircularization (IRR) of linearized plasmid DNA was used to study mechanistic rel

105 Complexes formed in vitro using linearized plasmid DNA were imaged and measured by atomi

106 Dmc1 renatures linearized plasmid DNA with first order reaction kinetic

107 DNA end-joining was measured by incubating linearized plasmid DNA with mitochondrial protein extrac

108 ent HR and random chromosomal integration of linearized plasmid DNA, a subtype of NHR, which we demon

109 Nuclear injection of linearized plasmid DNA, circular DNA with a large gap, o

110 We also report that the degradation of linearized plasmid DNA, even devoid of chi sites, is nev

111 o-transforming Escherichia coli strains with linearized plasmid DNA, sequencing the resulting junctio

112 percoiled plasmid DNA is more sensitive than linearized plasmid DNA.

113 vity and promotes intermolecular ligation of linearized plasmid DNA.

114 Mature phage T4 DNA and linearized plasmid DNAs containing or lacking a cloned T

115 scent protein) knockdown and a dsRed-express linearized plasmid for induction to be optically analyze

116 t a single base mismatch in a 2725 base pair linearized plasmid heteroduplex.

117 First, transfection of a linearized plasmid in which expression of the reporter g

118 , EcoRI and KpnI increased the efficiency of linearized plasmid integration up to 5-fold in CHO cells

119 The o-tRNA can be transcribed either from a linearized plasmid or from a crude PCR product.

120 cat deletion by natural transformation with linearized plasmid pPAN4 (catBCIJF) 10 mutants were unab

121 tant), the predominant reaction product is a linearized plasmid resulting from single end cleavage.

122 of fission yeast with the PCR fragment and a linearized plasmid vector prepared such that the ends of

123 nsformants from a pool of PCR products and a linearized plasmid vector.

124 gonucleotides are ligated onto the ends of a linearized plasmid which is then transformed into Saccha

125 We used recircularization of a linearized plasmid with 3-P-blocked termini, mimicking t

126 ded templates were generated by digestion of linearized plasmid with the double-strand-specific T7 ge

127 and RecD leads to processive unwinding of a linearized plasmid.

128 ecific with both a 272 bp DNA fragment and a linearized plasmid.

129 g hairpin ends that is not seen in repair of linearized plasmids and may be involved in positioning t

130 Small linearized plasmids appeared to be inserted into the nuc

131 ed capacity for correct recircularization of linearized plasmids by illegitimate end-joining after tr

132 Linearized plasmids carrying a Saccharomyces cerevisiae

133 d with various concentrations of circular or linearized plasmids containing different lengths of the

134 pacity of cells to recircularize transformed linearized plasmids correctly by non-homologous DNA end

135 We transformed yeast with a series of linearized plasmids to examine the role of Pol4 (Pol IV,

136 plementation groups A, C, D2, and G rejoined linearized plasmids with a significantly decreased effic

137 ScaI-linearized plasmids with single (pBS+) and double (pGEM-

138 s were prepared by in vitro transcription of linearized plasmids with T7 RNA polymerase.

139 id being supercoiled and was not observed in linearized plasmids, consistent with the presence of the

140 of LigD in vivo only mildly impairs NHEJ of linearized plasmids, indicating that an additional DNA l

141 s and N. gonorrhoeae, using serially diluted linearized plasmids.

142 nd in situ; on duplex DNA in supercoiled and linearized plasmids; and on oligonucleotide DNA/DNA and

143 Linearized plots of the percentages of (4)C1 against lim

144 using the finite difference solution to the linearized Poisson-Boltzmann equation (FDPB) and solvati

145 sed on the finite difference solution to the linearized Poisson-Boltzmann equation reproduce the obse

146 Calculations using the linearized Poisson-Boltzmann equation show that the effe

147 With finite difference solutions of the linearized Poisson-Boltzmann equation, we then calculate

148 y-combining site are computed by solving the linearized Poisson-Boltzmann equation.

149 merical and model dependent solutions of the linearized Poisson-Boltzmann equation.

150 uation approach is applied to discretize the linearized Poisson-Boltzmann equation; the resulting int

151 g: 1), full nonlinear Poisson-Boltzmann; 2), linearized Poisson-Boltzmann; and 3), neglect of solute

152 When linearized pPAC-ResQ vector and P1 or PAC DNA are cotran

153 Starting with a solution of the linearized problem about the flat rest state, a family o

154 Linearized pUC19 plasmid was ligated to an oligomer cont

155 The linearized rate of rejection (episodes of rejection/100

156 ion fraction > or =60% and sinus rhythm, the linearized rate of SUD was not different in functional c

157 The yearly linearized rate of sudden death was 1% in patients in fu

158 /- 2% and 18.8 +/- 4%, respectively, and the linearized rate was 1.8% per year.

159 +/- 3% and 48 +/- 6%, respectively, and the linearized rate was 5.0 +/- 0.7% per year.

160 Linearized rates (events/patient-year) were: reoperation

161 d plasmid concentration and was greater when linearized rather than circular plasmid DNA was used.

162 Linearized, recombinogenic pClasper targeting vector and

163 Therefore, the efficient transcription of linearized, relaxed templates found here demonstrates th

164 andom with DNase I, and incorporation of the linearized, repaired, blunt-ended, rearranged genes into

165 (2) To identify how the model generates this linearized response in a nonlinear network.

166 This interaction produces linearized responses that agree with both extracellular

167 of cAMP without the limitation of requiring linearized signals imposed by the 1D approach.

168 single (18)F-fallypride PET protocol and the linearized simplified reference region kinetic model in

169 PET data were analyzed using the linearized simplified reference region model, which acco

170 st NXS2 neuroblastoma by gene therapy with a linearized, single-chain (sc) version of the heterodimer

171 tissue-compartment graphical analysis with 6 linearized solutions of the fundamental binding equation

172 eir complements, creating a cascade in which linearized species accumulate exponentially.

173 ion and van der Waals forces is studied as a linearized stability problem in a purely irrotational an

174 race/ethnicity, and year, with Taylor series linearized standard errors clustered by state and classr

175 ty delivery, accounting for clustering using linearized standard errors.

176 reactions were performed with the bleomycin-linearized substrate.

177 hat blockade of transient potassium channels linearizes summation.

178 The linearized system depends upon Froude numbers reflecting

179 romyces cerevisiae between genomic DNA and a linearized TAR cloning vector containing targeting seque

180 promoter template was greater than that on a linearized template.

181 emonstrate that the resulting changes to the linearized temporal filters of this nonlinear network ma

182 a large membrane-bound protein (214 kDa when linearized) that mediates internalization of vitellogeni

183 such as A-type potassium currents, which can linearize the frequency-input current curve.

184 e DNA minus strand at specific sites and can linearize the minus strand via a non-homologous recombin

185 t that eukaryotic topoisomerase I (top1) can linearize the open circular DNA of duck hepatitis B viru

186 5-HT(3) receptor agonist-induced responses, linearized the current-voltage relationship, increased a

187 Human auditory efferent activation linearizes the cochlear response for mid-level sounds wh

188 asing the extracellular Ca(2+) concentration linearizes the current-voltage curve of the proctolin-in

189 mation of a calcium-carboxylate network that linearizes the helix and exposes the hydrophobic amino a

190 responding system of differential equations, linearizing the system about the steady state, and deter

191 r reduced activity, but when the plasmid was linearized, the enhancer-blocking activity was observed

192 ation near the membrane by extrapolating the linearized time dependencies of selectivity coefficients

193 by simple crossings-over between ends of the linearized transforming fragment and the target chromoso

194 ydrolyze the trilactone just once to produce linearized trimers.

195 dependence of Hb absorbance was effectively linearized using the current technique (R(2) = 0.990+/-0

196 were constructed by co-transformation with a linearized vector and a PCR-synthesized hoc gene.

197 When mixed together, the linearized vector and PCR products directionally assembl

198 vivo recombination between genomic DNA and a linearized vector containing homologous sequences, or ho

199 d affinity capture (TAC) for purification of linearized vector DNA, and (2) universal primer sequence

200 Electroporation with linearized vector gave approx. 10(6) transformants per m

201 triction enzyme is electroporated along with linearized vector into cells, thus determining the site

202 nction sites reveals that integration of the linearized vector occurred with minimal rearrangement of

203 onstructed in vivo by co-transformation with linearized vector plus insert DNAs, followed by intermol

204 piece of gel, and the gel extraction of the linearized vector.

205 long with two genetically marked (M1 and M2) linearized vectors that contained a human Alu sequence a

206 ing nearly two hundred oligonucleotides to a linearized version of the m13mp18 virus genome.

207 eling, particularly those based on classical linearized viscoelasticity.

208 ds, in its entirety, on the structure of the linearized wave equation.

209 cular, supercoiled DNA vector pUC19 is first linearized with a restriction enzyme to create a sequenc

210 tions were crosslinked with psoralen and UV, linearized with restriction enzymes and spread for EM vi

211 S1nuclease treatment of replication products linearized with single cutting restriction enzymes, foll

212 ing intensity is greater when the plasmid is linearized with the TRS at an end (39-49%) than when lin

213 ed with the TRS at an end (39-49%) than when linearized with the TRS in the interior (18-22%).

214 PCR-amplified with 30-40 bp of homology to a linearized yeast plasmid.

215 used for rapid and efficient cloning into a linearized yeast vector that contains these same element