ライフサイエンスコーパス: lipidate

1 from lipA, lipB, and lipA lipB mutants were lipidated.

2 protein was found to be surface exposed and lipidated.

3 n activity, the substrate is intracellularly lipidated.

4 ane-associated form of apoB-100 is partially lipidated.

5 Combining one recombinant lipidated 2086 (rLP2086) variant from each subfamily wit

6 the lysine resonances in the spectrum of the lipidated 22-kDa fragment were made.

7 results were observed in mice immunized with lipidated (56%-98%; mean, 69%; SD, 13.6%) and combinatio

8 munization, we have developed an approach of lipidating a multiple Ag peptide (MAP) containing part o

9 etermined hydrodynamic properties of the non-lipidated AcrA and found that AcrA exists in solution as

10 However, lipidated Ag delivered in microparticles induced higher

11 , expression of a palmitoylation-independent lipidated AKAP mutant in DHHC2-deficient neurons largely

12 Treatment of mice with lipidated albumin to induce proteinuria caused a decreas

13 cture-activity studies revealed that certain lipidated alpha-peptide/beta-peptoid hybrid oligomers po

14 pecific interaction of a fluorophore-labeled lipidated alpha-peptide/beta-peptoid hybrid with monocyt

15 combinant forms of the antigen having either lipidated amino termini, approximating the natural secre

16 luble membrane anchored ligand, we generated lipidated analogs of the 9-amino acid fragment.

17 Purified non-lipidated and intact forms of AcrA were able to restore,

18 Finally, we demonstrate that lipidated and non-lipidated apoA-I significantly decreas

19 amino-terminal domain (HhN), which is dually lipidated and secreted as part of a multivalent lipoprot

20 tion, all simulated double belts of the four lipidated apoA-I particles have helical domains that imp

21 nally, we demonstrate that lipidated and non-lipidated apoA-I significantly decreased Abeta toxicity

22 us formation of more than one type of poorly lipidated apoA-I-containing particle: there were 9- and

23 idation as they secrete both unlipidated and lipidated apoB forms despite their lack of microsomal tr

24 h amyloid plaques, demonstrating that poorly lipidated apoE co-deposits with insoluble Abeta.

25 timal receptor-binding activity by the fully lipidated apoE during lipoprotein transport in circulati

26 urity in preventing lipid-free and partially-lipidated apoE from premature binding to apoE receptors

27 CX, whereas levels of lipoprotein-associated/lipidated apoE have the opposite pattern (6 months).

28 onate-insoluble apoE, suggesting that poorly lipidated apoE is less soluble in vivo.

29 pite substantially lower apoE levels, poorly lipidated apoE produced in the absence of ABCA1 is stron

30 Together, these data show that lipidated apoE, especially apoE4, increases Abeta oligom

31 The enhanced expression of lipidated ApoE, through the activation of liver X recept

32 ding cassette family of active transporters, lipidates apoE in the CNS.

33 With lipidated apoE3, it is confirmed that the Lys-233 site i

34 s display a marked elevation in secretion of lipidated apolipoprotein B-100.

35 In mouse primary neuronal culture, lipidated apolipoprotein E4 enhances oligomeric amyloid-

36 Both peptides were lipidated at the N-terminus by transfer from PC, phospha

37 biquitylated proteasome subunits/targets and lipidated ATG8 lining the enveloping autophagic membrane

38 autophagosomal membrane by interacting with lipidated ATG8 proteins (LC3/GABARAP) that are intimatel

39 sed by host autophagy proteins engaging only lipidated Atg8 proteins.

40 GABARAP, unlipidated and lipidated, but not LC3B, GABARAPL1, and GATE-16, specifi

41 ng translocation, bacterial lipoproteins are lipidated by lipoprotein diacylglycerol transferase (Lgt

42 a and mycobacteria, lipoproteins are further lipidated by lipoprotein N-acyl transferase (Lnt), to gi

43 tilization and suggest a broader role of the lipidated C terminus in Ha-Ras biological functions.

44 :1 complex with Rab3A that requires both the lipidated C terminus of Rab3A and the presence of bound

45 Arl3 is involved in targeting and releasing lipidated cargo proteins from their carriers PDE6delta a

46 into liposomal membranes, a small library of lipidated coiled coil forming peptides was designed in w

47 hat existing membranes can be activated with lipidated coiled coil forming peptides, which might lead

48 t apoE with apoE4 lipoprotein particles less lipidated compared to apoE3 particles.

49 is lipoprotein-associated, and perhaps less lipidated, compared with apoE2 and apoE3.

50 nsity lipoprotein receptor when present on a lipidated complex.

51 s a higher membrane affinity than its doubly lipidated counterpart, or whether the affinity contribut

52 Echinocandins are a family of fungal lipidated cyclic hexapeptide natural products.

53 Membrane localization of lipidated cytosolic signaling proteins is mediated by in

54 nce-based assays indicated pore formation by lipidated DeltaCR_PrP, a variant that is known to be hig

55 ucturally diverse bacterial products such as lipidated factors (lipoproteins and peptidoglycans) and

56 tical modeling, and in vitro measurements of lipidated fluorescent peptide dissociation from vesicles

57 mposition which can incorporate a variety of lipidated fluorophores and work well as fluorescence sta

58 nds to LC3 of both cytosolic form (LC3I) and lipidated form (LC3II).

59 esent study we tested whether a cell-derived lipidated form of apoE can alter neurite outgrowth in th

60 Moreover, the lipidated form of LC3, LC3-II, which triggers autophagy

61 gy-cargo protein p62/sequestosome 1, and the lipidated form of microtubule-associated protein light c

62 The lipidated form of MxiM was detected primarily in the out

63 demonstrate that apoA-I in lipidated or non-lipidated form prevents the formation of high molecular

64 ition to free ATG8, its membrane-associated, lipidated form was detected in many maize tissues, sugge

65 c conversion of autophagy protein LC3 to its lipidated form.

66 found that both enzymatically and chemically lipidated forms of GATE-16 and GABARAP proteins promote

67 on (NNR) measurements have been made for two lipidated forms of GlyCys, interacting with analogues of

68 e we report for the first time the design of lipidated gamma-AApeptides as antimicrobial agents.

69 ucture reveals one membrane contact near the lipidated Ggamma C terminus and Galpha N terminus, and a

70 d to evaluate the potential of a recombinant lipidated group B meningococcal outer membrane protein P

71 an 10% of the transforming activity of fully lipidated H-Ras(61Leu) in NIH 3T3 cells.

72 brane rafts in cholesterol efflux applies to lipidated HDL particles but not to lipid-free apoA-I.

73 lular mechanisms that have evolved to handle lipidated Hh proteins in the spatial deployment of the s

74 rst identified as a putative lipoprotein, is lipidated in vivo.

75 ally, we showed that secreted E4 is the most lipidated isoform in astrocytes, suggesting that increas

76 concentration-dependent fashion by purified lipidated K-Ras reconstituted in PtdIns(4,5)P(2)-contain

77 nsive membrane tethering and fusion, whereas lipidated LC3 does so to a much lesser extent.

78 85 results in encapsulation of phagosomes by lipidated LC3 in multi-membrane organelles.

79 However, while lipidated LC3 levels were elevated regardless of T4SS ac

80 ic vacuoles, an increase in the level of the lipidated LC3 protein, and the formation of autolysosome

81 uces autophagy, as judged by accumulation of lipidated LC3-II, and that this induction occurs rapidly

82 Autophagy is detected by the appearance of lipidated light chain 3 (LC3) and by increases in puncta

83 c response were based on the accumulation of lipidated light chain 3 (LC3), an autophagosome marker,

84 Inactive in the membrane: Lipidated light-responsive constructs that sequester bio

85 s for the requirement of arachidonoyl PLs in lipidating lipoprotein particles.

86 d wild-type (+/+) mice were immunized with a lipidated lymphocytic choriomeningitis virus (LCMV) pept

87 In contrast, lipidated MAP delivered by nasal route microparticles wa

88 this work, we compare two delivery systems, lipidated MAP in PBS and encapsulation in poly(DL-lactid

89 However, whether the triply lipidated membrane anchor of H-ras has a higher membrane

90 As model systems, we chose the triply lipidated membrane targeting motif of H-ras (tH) and a l

91 l vaccine delivered by Escherichia coli as a lipidated, membrane-associated protein.

92 CD14 physically delivers these lipidated microbial products to various TLR signaling co

93 As a model system we used the lipidated minimal membrane anchor of the GTPase, N-Ras (

94 nstrated a role for both in secretion of the lipidated morphogen Hedgehog.

95 sults demonstrate the feasibility of using a lipidated multimeric peptide for mucosal immunization to

96 t RcsF has a transmembrane topology with the lipidated N terminus on the cell surface and the C-termi

97 t to be tethered to the inner leaflet by its lipidated N terminus, raising the question of how it mon

98 ed to the outer membrane bilayer through its lipidated N terminus.

99 to adopt a transmembrane orientation with a lipidated N-terminal domain on the cell surface and a pe

100 in (MTP)-negative cell line that can neither lipidate nor efficiently secrete apoB28.

101 linked immunosorbent assays with recombinant lipidated OMP G1a to detect antibodies made specifically

102 y the Sec general secretory pathway and then lipidated on a conserved cysteine by the enzyme lipoprot

103 Here we demonstrate that apoA-I in lipidated or non-lipidated form prevents the formation o

104 ptides (15 mers), in various formulations as lipidated or nonlipidated multiantigenic peptides or as

105 TNF-alpha release was not induced by either lipidated or unlipidated recombinant OspA.

106 role of these disulfides in the secretion of lipidated or unlipidated truncated forms of apoB was stu

107 -1 human monocytic cell line and recombinant lipidated OspA (L-OspA) as the model target cell and sti

108 monocytic cell line and purified recombinant lipidated OspA (L-OspA) were used as the model target ce

109 hetes (freeze-thawed, live, or sonicated) or lipidated outer surface protein A (L-OspA) augmented the

110 -killed Borrelia burgdorferi spirochetes and lipidated outer surface protein A (L-OspA) stimulated th

111 Purified lipidated outer surface protein A (OspA), but not its un

112 human monocytes with B. burgdorferi lysate, lipidated outer surface protein A, and triacylated lipop

113 The vaccine, which is derived from a lipidated outer surface protein of the causative spiroch

114 2) ApoB:986 formed both a lipidated particle that was denser than HDL(3) and large

115 oaffinity column and was present in a poorly lipidated particle with a density between 1.19 and 1.26

116 ificantly diminished capacity to form intact lipidated particles and (ii) increased propensity to for

117 At these lipid lengths, the N-lipidated peptide dimers exhibited strong LPS permeabili

118 We systematically designed a series of N-lipidated peptide dimers that are active against Gram-ne

119 Short lipidated peptide sequences derived from various intrace

120 ic liposomes in situ, i.e., by addition of a lipidated peptide solution to plain liposomes.

121 ized by proteolytic processing (shedding) of lipidated peptide termini in vitro.

122 residues remained equally reactive when the lipidated peptide was exposed to acrolein.

123 issociation constants of binding between the lipidated peptides and serum albumin.

124 ociation constants of albumin-binding of our lipidated peptides are all very close and similar to tha

125 The monomers of lipidated peptides bind to albumin.

126 Our findings suggest that the monomeric lipidated peptides bind to HSA mainly by the fatty acid

127 Cell-penetrating lipidated peptides called pepducins target specific intr

128 such molecules, we screened approximately 50 lipidated peptides derived from the intracellular loops

129 These lipidated peptides differ in the N-terminal mutation, th

130 Complementary coiled coil forming lipidated peptides embedded in liposomal membranes are a

131 We found that these lipidated peptides form stable oligomers at concentratio

132 liposomes are prepared by mixing lipids and lipidated peptides in organic solvent (e.g., chloroform)

133 anchor is vital for the correct insertion of lipidated peptides into liposomal membranes, a small lib

134 We designed lipidated peptides that are anchored to the surface of r

135 In favorable cases, lipidated peptides were further characterized by tandem

136 These lipidated peptides--which we have termed pepducins--requ

137 ism of the prolonged circulating duration of lipidated pharmaceutical peptides.

138 Among these, the first synthesis of a fully lipidated PIM (i.e., (16:0,18:0)(19:0/16:0)-PIM(2)) was

139 ne involves electrostatic interaction of the lipidated polybasic domain with anionic phospholipids in

140 translocation and surface expression of the lipidated polymer.

141 n of both the nonlipidated substrate and the lipidated product.

142 Insertion of the recombinant, non-lipidated protein into artificial membranes results in b

143 It is unclear how this dually lipidated protein is transported over a long range in th

144 e regulatory role of membrane composition in lipidated protein membrane targeting.

145 le the lipid modification was essential, the lipidated protein was more potent than a lipid-modified

146 iency causes lysosomal accumulation of these lipidated proteins (major constituents of ceroid) leadin

147 the potency of membrane curvature to recruit lipidated proteins and thereby synergistically regulate

148 Wnt proteins are secreted, hydrophobic, lipidated proteins found in all animals that play essent

149 Sorting and trafficking of lipidated proteins has traditionally been discussed in t

150 l needed to biochemically characterize these lipidated proteins in specific cell types and disease st

151 Despite the abundance of lipidated proteins in the human proteome, the key determ

152 ins, as well as the discovery of unannotated lipidated proteins such as isoform-specific S-farnesylat

153 d increases the endomembrane localization of lipidated proteins that are cytoplasmic cargo in the exo

154 re synthesized as dually (N- and C-terminal) lipidated proteins that firmly tether to the surface of

155 Recombinant non-lipidated proteins were purified by affinity or ion exch

156 vert these otherwise hydrophilic proteins to lipidated proteins, thus facilitating membrane associati

157 ally regulate the trafficking and sorting of lipidated proteins.

158 he capacity of membrane curvature to recruit lipidated proteins.

159 ure can in itself mediate the recruitment of lipidated proteins.

160 osition synergize to modulate recruitment of lipidated proteins.

161 1, respectively) are the most important apoE-lipidating proteins.

162 Analyses of the interactions of lipidated PrPs with phospholipid membranes demonstrated

163 PrP to membranes, distinct from that of non-lipidated PrPs, and influences the biochemical and confo

164 Synthetically lipidated recombinant green fluorescent protein (GFP) wa

165 rom HUVEC incubated with OspA lipopeptide or lipidated recombinant OspA induced chemotaxis of neutrop

166 ults were obtained with 25 nM native OspA or lipidated recombinant OspA or OspB.

167 tance resulting from the administration of a lipidated recombinant outer surface protein A (OspA) vac

168 ion to pressure raises the idea of a role of lipidated signaling molecules as mechanosensors, transdu

169 spectroscopically resolved substates of the lipidated signaling protein N-Ras.

170 Lipidated small GTPases and their regulators need to bin

171 structure of Escherichia coli CsgG in a non-lipidated, soluble form as well as in its native membran

172 n A-I in the circulation is transported in a lipidated state and ABCA1-dependent efflux to individual

173 ts of binding among the apoE isoforms in the lipidated state.

174 The structure of the lipidated terminus dictates a unique assembly mechanism

175 factor for Alzheimer's disease (AD), is less lipidated than its corresponding AD-benign form, apoE3,

176 microarrays presenting spots of collagen and lipidated tissue factor (TF) allowed a determination of

177 -mum long patch of type I fibrillar collagen/lipidated tissue factor (TF; approximately 1 TF molecule

178 bbit following intravenous administration of lipidated TN-ApoA1.

179 the ER membrane, where it becomes partially lipidated to form a primordial intermediate.

180 g proteins that must be post-translationally lipidated to function as molecular switches in signal tr

181 he endoplasmic reticulum (ER) and is further lipidated to very low density lipoproteins (VLDL).

182 0-lipoproteins in ER vesicles had not become lipidated to VLDLs.

183 n in eukaryotes that is covalently attached (lipidated) to the elongating autophagosomal membrane.

184 treponemal lipoproteins are hydrophilic, non-lipidated TP0453 can integrate into membranes, a propert

185 e unconjugated hybrid peptide as well as its lipidated variants.

186 Here we show that lipidated versions of the antigens elicited significantl

187 for the reassembly of triacylglycerols that lipidate very low density lipoprotein particles as part

188 This was confirmed using lipidated vs nonlipidated hexapeptides of Borrelia outer

189 Since several of these proteins are lipidated, we hypothesized that their immunogenicity and

190 ion studies using RSPO ligands and a new non-lipidated Wnt analogue reveal that these ligands have qu

191 Furthermore, these easily produced, non-lipidated Wnt surrogate agonists facilitate functional s