ライフサイエンスコーパス: living cell

1 d and fascinating process occurring in every living cell.

2 leavages by major endoribonucleases within a living cell.

3 oring the nonequilibrium metabolic status of living cell.

4 alpha-syn phosphorylation and degradation in living cells.

5 and herpes simplex virus (HSV) particles in living cells.

6 fective inhibitors of aerobic respiration in living cells.

7 that require the culture and manipulation of living cells.

8 nly nanodevices to evaluate complex logic in living cells.

9 ngle proteins in vitro and inside individual living cells.

10 clear volume regulation and thermosensing in living cells.

11 in about where, when, and how this occurs in living cells.

12 le elastic properties of soft materials like living cells.

13 is an essential process for the survival of living cells.

14 soft samples in aqueous environments such as living cells.

15 e shape of intracellular energy gradients in living cells.

16 in the nucleoplasm, and in the cytoplasm of living cells.

17 induced in Mn(2+) -limited cultures of free-living cells.

18 e structure during chromosome segregation in living cells.

19 onitor the real-time glutathione dynamics in living cells.

20 t the maximum quantum efficiency possible in living cells.

21 ay to study protein-receptor interactions on living cells.

22 RNAs (tRNAs) perform essential tasks for all living cells.

23 al data within the genomes of populations of living cells.

24 zyme complexes that regulate glucose flux in living cells.

25 ith purified receptors on glass surfaces and living cells.

26 protein interactions and their interfaces in living cells.

27 lipids on micron scales within unperturbed, living cells.

28 abel differentially necrotic HeLa cells from living cells.

29 it is unclear how IL-1 can be secreted from living cells.

30 hly specific and sensitive quantification in living cells.

31 quantifying their relative colocalization on living cells.

32 s dominate all major biological processes in living cells.

33 lar monoamine neurotransmitters detection in living cells.

34 for CaM preassociation to NMDA receptors in living cells.

35 allow nanoscale interactions to be probed in living cells.

36 the behavior of individual molecules inside living cells.

37 mical function within the plasma membrane of living cells.

38 le replication of the strand in vitro and in living cells.

39 al-time monitoring of signal transduction in living cells.

40 and unbinding) in the in vivo environment of living cells.

41 f ER-mitochondrial associations in fixed and living cells.

42 for measurement and control of molecules in living cells.

43 d to available nutrients is critical for all living cells.

44 me monitoring of biological processes within living cells.

45 nsforming RNA secondary structure studies in living cells.

46 omised for forming stable subnuclear foci in living cells.

47 rown from the ability to edit genomes within living cells.

48 geography of IP3Rs and Ca(2+) signals within living cells.

49 isualize dynamic processes and structures in living cells.

50 lved measurement of GPCR agonist response in living cells.

51 ans (GAGs)-specific targeting and imaging of living cells.

52 l receptors and G proteins at the surface of living cells.

53 Transcription is an essential process in all living cells.

54 resented for controlling DNAzyme activity in living cells.

55 naked-eye tracking glycolytic inhibition in living cells.

56 target interrogation are not well defined in living cells.

57 t of equilibrium thermodynamic parameters in living cells.

58 le of supporting, and inhabited by the first living cells.

59 elling strategy for intracellular targets in living cells.

60 vitro and for protein-protein conjugation in living cells.

61 vity in the optical monitoring of voltage in living cells.

62 interactions (PPIs) govern most processes in living cells.

63 f these sensors both in the test tube and in living cells.

64 udies show an indispensable role for Hrd3 in living cells.

65 ajor repressor of Nrf2, both in vitro and in living cells.

66 as an enzyme that regulates FA metabolism in living cells.

67 states of proteins within defined regions of living cells.

68 complex hybrid synthetic genetic programs in living cells.

69 nalyzing protein-protein interactions within living cells.

70 ar highways" for long-range transport within living cells.

71 ssociations as well as proximate proteins in living cells.

72 are capable of behavior approaching that of living cells.

73 e large cytosolic dynein/dynactin complex in living cells.

74 and its ability to selectively detect Cys in living cells.

75 pid stimulation-mediated ceramide release in living cells.

76 nd understanding cytosolic lipid droplets in living cells.

77 profile mucin-type O-glycans synthesized by living cells.

78 r chaperones in assisting protein folding in living cells.

79 ing protein 2 (CRABP2), both in vitro and in living cells.

80 on that occurs on proteins and lipids in all living cells.

81 , as well as modulated the morphodynamics of living cells.

82 ghts into native protein-RNA interactions in living cells.

83 chemistry of dynamic signalling complexes in living cells.

84 nge of fluorinated small-molecule targets in living cells.

85 zes information on RNA structure obtained in living cells.

86 al nanoagent for molecular investigations in living cells.

87 ies to avoid hydraulic failure and damage to living cells.

88 ization of individual tagged proteins inside living cells.

89 o both visualize and perturb biochemistry in living cells.

90 r understanding their biological function in living cells.

91 ic processes are ubiquitous and essential in living cells.

92 uires studies of capsid assembly dynamics in living cells.

93 3D dynamics of the endoplasmic reticulum, in living cells.

94 erform nanoscale pulldowns (NanoSPDs) within living cells.

95 o activate IDR-mediated phase transitions in living cells.

96 cal and pathological roles of glutathione in living cells.

97 sted for the real-time detection of Cyt c in living cells.

98 unit interactions with temporal precision in living cells.

99 alternatives to the metabolic engineering of living cells.

100 , and to detect individual mRNA in fixed and living cells.

101 f hIRE1alpha LD abolished IRE1's activity in living cells.

102 mic protein-protein interaction complexes in living cells.

103 shown that they also play a key role within living cells.

104 that all these modes are operational inside living cells.

105 pping the uptake of molecules of interest at living cells.

106 dium transfer hydrogenation catalysts inside living cells.

107 asuring the instantaneous lipogenesis of the living cells.

108 e (EGFP-K85AcK) that responds to sirtuins in living cells.

109 method to study virus particle transport in living cells.

110 d receptor (GR) in the interphase nucleus of living cells.

111 ach for engineering evolving DNA barcodes in living cells.

112 activity, and lipid domains is difficult in living cells.

113 at CENP-F tracks growing microtubule ends in living cells.

114 ue for applying local mechanical stresses to living cells.

115 fluorescence imaging of mt-Keima is 20 h for living cells.

116 the interaction of Nef with AP-2 and CD4 in living cells.

117 o a lack of assays to assess BAF function in living cells.

118 targeted APEX2 to biotinylate LD proteins in living cells.

119 terns and the aggregate kinetics observed in living cells.

120 e desmosomal cadherin desmoglein 3 (Dsg3) in living cells.

121 ar transport in artificial systems (1) or in living cells (2) .

122 varied and subtle roles that water plays in living cells-a consideration that must be free of both a

123 unique feature of rapid internalization into living cells, after which they retain their fluorescence

124 Analysis was performed on single living cells along with dehydrated and fixed cells to pr

125 replicate exclusively within the interior of living cells, an osmotically protected niche.

126 In this work the interactions between a living cell and a nano-object, and in particular the eff

127 fluorescence imaging of SoNar is 30 min for living cells and 60 min for living mice.

128 nerating stimulated emission directly inside living cells and animal tissues.

129 roperties of apical membranes separated from living cells and attached to a porous mesh in the absenc

130 both exogenous and endogenous H2O2 levels in living cells and can further be coupled with glucose oxi

131 lytic activity of TEM1-beta-lactamase inside living cells and compared the values to those obtained i

132 Xist at single-nucleotide resolution both in living cells and ex vivo.

133 ssembly, we investigated its biosynthesis in living cells and faithfully reconstituted the underlying

134 n of spatiotemporal changes in GTP levels in living cells and for high-throughput screening of molecu

135 stic tool for analyzing glycan expression on living cells and further helps cancer diagnosis and trea

136 viscoelastic properties of soft samples like living cells and hydrogels directly from conventional AF

137 omparison with the existed AFM techniques on living cells and hydrogels.

138 oncentration and enzymatic activities inside living cells and in in vitro applications.

139 lesterol (Chol)- driven nanodomains exist in living cells and in model membranes.

140 svirus procapsids and their morphogenesis in living cells and indicate that the encapsidation machine

141 timulated Raman scattering imaging of single living cells and mass spectrometry analysis of extracted

142 Living cells and the extracellular matrix (ECM) can exhi

143 ng TF assembly at cis-regulatory elements in living cells and the long-range molecular "dialog" betwe

144 d muscle relies on the compatibility between living cells and their muscle scaffolds during cell cult

145 a universally defining characteristic of all living cells and tissues and is intrinsically linked wit

146 eful tools for monitoring cellular events in living cells and tissues.

147 vity microdomains on the plasma membranes of living cells and to uncover the role of clustered anchor

148 ly of supramolecular structures is vital for living cells, and a central challenge for engineering at

149 ined by fluorescence recovery experiments in living cells, and chromatin immunoprecipitation (ChIP) a

150 RecBCD's biologically important reaction in living cells, and enable more precise analysis of Chi's

151 ectivity for hypoxic activation in vitro, in living cells, and in multiple disease models in vivo.

152 endogenous polymerase clustering dynamics in living cells, and our approach may in principle be used

153 ed the accumulation of misfolded proteins in living cells, and reduced the cytotoxicity of SOD1(G93A)

154 or measuring dynamic protein interactions in living cells, and show that preferential DRD3 signaling

155 wever, few tools exist to examine the INM in living cells, and the INM-specific proteome remains poor

156 h LDs having different biofunctionalities in living cells, and thus could be inaccurate in measuring

157 for quantifying and visualizing analytes in living cells, and when targeted to organelles have the p

158 Our intermolecular FRET measurements in living cells are consistent with beta-arrestin binding t

159 Living cells are treated with the icSHAPE chemical NAI-N

160 uely, the pool of RNAs to which tau binds in living cells are tRNAs.

161 Tubular protrusions are a common feature of living cells, arising from polymerization of stiff prote

162 ctronic measurements of reporter proteins in living cells as an alternative to traditional optical fl

163 d that SERS provides a deep understanding of living cells as well as their microenvironment which is

164 he biological interactions between Si3N4 and living cells, as a consequence of the off-stoichiometric

165 d to visualize dynamic protein activities in living cells at a resolution commensurate with cellular

166 ensors that detect biochemical activities in living cells at a resolution up to threefold better than

167 g the fundamental structure and processes of living cells at the nanoscale poses a unique analytical

168 e CRISPRainbow, a system for labeling DNA in living cells based on nuclease-dead (d) Cas9 combined wi

169 as been problematic to test this proposal in living cells because these surface structures are sub-li

170 able dye pair for two-colour STED imaging in living cells below 50 nm resolution.

171 Label-free imaging of living cells below the optical diffraction limit poses g

172 to interpret the viscoelastic properties of living cells best.

173 ation of protein-protein interactions inside living cells, but existing methods are mostly limited to

174 ty to write information into the genome of a living cell by the addition of nucleotides over time.

175 tase (DHFR) transgene can be visualized in a living cell by using three-dimensional magnetic twisting

176 p the intracellular mechanical properties of living cells by combining micropatterning and optical tw

177 Computation can be performed in living cells by DNA-encoded circuits that process sensor

178 o single-molecule assessment of RNA decay in living cells by exploiting the ability of flavivirus RNA

179 ally influenced corrosion (EMIC), from other living cells by interspecies electron transfer (IET), or

180 sed framework for building state machines in living cells by leveraging chemically controlled DNA exc

181 maging cellular structures and organelles in living cells by long time-lapse super-resolution microsc

182 (Young's) modulus of soft samples, including living cells, by atomic force microscopy (AFM).

183 Imaging molecules of interest in living cells can elucidate how molecules enter cells, tr

184 escently labeled drugs at high resolution in living cells can reveal important details about how drug

185 Inspired by nature, where active living cells coexist with lifeless objects and structure

186 Living cells contain diverse biopolymers, creating a het

187 Dense monolayers of living cells display intriguing relaxation dynamics, rem

188 o represents a significant challenge because living cells do not take up immunoglobulins to cellular

189 opens a way to quantify lipid metabolism in living cells during cellular development and transition.

190 Polyphosphates have occurred in living cells early in evolution and microalgae contain t

191 down to 1%, and increased the consistency of living cells elasticity measurements by a factor of two.

192 (1)O2 generation by BADs in living cells enables visualization of the dyads distribu

193 amics of molecular processes taking place in living cells, especially upon external stimulation, in a

194 gh affinity in vitro and high specificity in living cells, exhibited substantial cytotoxicity towards

195 antibodies that target membrane antigens of living cells frequently develop in cGVHD patients and fu

196 because it could discriminate proliferative living cells from a more composite mass consisting of tu

197 However, how living cell functions can be modulated via opsins by mod

198 Detection of specific mRNA in living cells has attracted significant attention in the

199 sualize and quantify transcription in single living cells has been essential in revealing the transcr

200 or normal caveolar structure and dynamics in living cells has been lacking.

201 localization at the single-molecule level in living cells has been predominantly achieved by engineer

202 techniques to image multiple genomic loci in living cells has limited our ability to investigate chro

203 visualization of endogenous genomic loci in living cells has proven to be laborious until the recent

204 aging in this thick polysaccharide matrix on living cells has significant promise in the drive to elu

205 Unchecked amino acid accumulation in living cells has the potential to cause stress by disrup

206 ral environment of a biomolecule is inside a living cell, hence, this is the most relevant environmen

207 t undergo acid-activated endosomal escape in living cells.Hydrogen bonding plays a major role in dete

208 ing and Gli-controlled gene transcription in living cells (IC50 = 230 nM), providing the most potent

209 s as well as their successful transport into living cells illustrate the promising potential of the s

210 e as alkyne-state-dependent Raman probes for living cell imaging due to synergetic enhancement effect

211 s establish RCas9 as a means to track RNA in living cells in a programmable manner without geneticall

212 her words, we interpret its reciprocity with living cells in chemical terms.

213 used to create pores within the membrane of living cells in order to deliver a substance, for exampl

214 with and diffuse on the plasma membranes of living cells in the absence of any receptor binding.

215 due to their potential functional impact on living cells in vivo.

216 t can be used for functional manipulation of living cells, including protein inactivation, targeted-d

217 In living cells intracellular proteolysis is crucial for pr

218 The cytoplasm of a living cell is a dynamic environment through which intra

219 hile the reciprocity between bioceramics and living cells is complex, it is principally governed by t

220 Stochastic motion on the surface of living cells is critical to promote molecular encounters

221 roplet level and how it evolves over time in living cells is essentially unknown due to the lack of s

222 Extracellular matrix stiffness sensing by living cells is known to play a major role in a variety

223 The regulation of size, volume and mass in living cells is physiologically important, and dysregula

224 Measuring BER capacity in living cells is valuable for both basic science applicat

225 It paves to enrich a life without affecting living cells leading to a possible survival of the patie

226 ine precise sequence of events at the single living cell level.

227 otect against undesired protein aggregation, living cells maintain a population of molecular chaperon

228 Subsequent interactions with living cells, may result in pathological responses such

229 de direct evidence of Wnt3A interaction with living cell membranes, and represent, to our knowledge,

230 opment in multicellular organisms, groups of living cells must often move collectively.

231 Living cells of X. autotrophicus may be directly applied

232 dge, angular approach SICM allows imaging of living cells on nontransparent substrates and a seamless

233 abled the label-free, real-time detection of living cells on the modified sensor surface under contro

234 s to rapidly produce small aggregates inside living cells on the order of seconds, as well as monitor

235 or GPI-APs, we detect two molecular pools in living cells; one pool shows high mobility with transien

236 s catalyzing halo-metabolite biosynthesis in living cells or generating and following the fate of tag

237 ular process can be deciphered from a single living cell other than a cell population.

238 e describe how to monitor mitophagic flux in living cells over an 18-h time frame, as well as how to

239 challenge to quantitatively analyse this in living cells over time, as opposed to studying snap-shot

240 In living cells, overexpression of endophilin delayed both

241 et formation or the average concentration of living cells per droplet to be controlled and kept const

242 his tripartite split-GFP system to assess in living cells protein-protein interactions in a dynamic c

243 The surface of a living cell provides a platform for receptor signaling,

244 mic changes of glutathione concentrations in living cells remain largely unknown.

245 details of their kinetics within individual living cells remain largely unknown.

246 s; however, detection of NADPH metabolism in living cells remains technically challenging.

247 relate to distinct steps in CCP formation in living cells remains unknown.

248 escent probes, antibodies, or plasmid DNA to living cells requires overcoming the plasma membrane bar

249 To visualize single-virus fusion in living cells, researchers take advantage of the proteoly

250 id to N-glycans of glycoprotein acceptors of living cells resulting in long-lived display.

251 allows for rapid real-time acquisition of a living cell's mechanical responses to forces via specifi

252 o combine the high-throughput biochemical or living cell screenings using the droplet microarray plat

253 Our measurements suggest that all living cells show fast and subtle mass fluctuations thro

254 measuring the elastic properties of gels and living cells, showing that its application reduces the v

255 rfaces for ultrasensitive detection of NA in living cells such as PC12.

256 ernative when working with valuable and rare living cells, such as primary cells or stem cells.

257 We show here by direct visualization in living cells that both cargos transit primarily the same

258 induction of protein-based hydrogels inside living cells that explores the chemically inducible dime

259 n vivo, allowing one to address questions in living cells that were previously restricted to reconsti

260 Here we visualize within a living cell the pore-size dependent deformation of a spe

261 Due to the thermodynamic openness of a living cell, the inability to instantaneously match fluc

262 y state between disulfides and thiols in all living cells, the collapse of the dendrimers to a multit

263 actions foster potent rhomboid inhibition in living cells, thereby opening avenues for rational desig

264 f stress-induced physiological conditions in living cells, therefore, requires real-time monitoring o

265 subject to complex mechanical constraints in living cells, these results provide important insights i

266 chemical quantification of endogenous H2S in living cells, thus hold great promise in the analytical

267 ing and observation of specific molecules in living cells, tissues and even entire organisms.

268 sticated tools to perturb specific lipids in living cells to assess the consequences for caveolae.

269 ynthetic gene circuits allow the behavior of living cells to be reprogrammed, and non-coding small RN

270 ural models using quantitative microscopy in living cells to investigate the hierarchy and temporal i

271 significantly increases the permeability of living cells to K(+), and forms K(+)- and Ca(2+)-selecti

272 ificial vesicles of defined composition into living cells to probe the capacity of the cellular cytop

273 s of hVDR and hRXRalpha-tagged constructs in living cells together with endogenous and tagged protein

274 Hence, large-scale membrane organization in living cells under physiologically relevant conditions c

275 uces single-nucleotide changes in the DNA of living cells using a fusion protein containing a catalyt

276 o disrupt p53/HDM2 and p53/HDMX complexes in living cells using a new recombinase enhanced bimolecula

277 IE (PKIE) during enzymatic AA oxygenation by living cells using a newly synthesized library of deuter

278 g of the rapid dynamics of miRNA activity in living cells using destabilized fluorescent proteins (ds

279 ogy for multiparametric kinase biosensing in living cells using FRET-FLIM.

280 ategy to visualize single RNA transcripts in living cells using molecular beacons (MBs) - fluorogenic

281 anine, into a neurotransmitter receptor in a living cell, utilizing a glutamate-gated chloride channe

282 Quantification of Cyt c within living cells via QDs is, however, influenced by various

283 lecule based super-resolution imaging in the living cells, we captured endogenous Pol II clusters.

284 this study, using high-resolution imaging in living cells, we discover that spindle movement is regul

285 orescence fluctuation spectroscopy in single living cells, we reveal that NAD(+) metabolism in the nu

286 tunable manipulation of PI(4,5)P2 levels in living cells, we show that depletion of PI(4,5)P2 comple

287 Here, using pH imaging in single living cells, we show that following acid stress, bacter

288 d subunit counting in the plasma membrane of living cells, we show that TREK1, TREK2, and TRAAK readi

289 uch dynamical analysis is also applicable in living cells, where it offers another dimension on the c

290 mation of multienzyme metabolic complexes in living cells, which appears to be controlled by post-tra

291 optimized using FRET standards expressed in living cells, which enables the determination of spectra

292 d the drug-release process to be followed in living cells with a significantly reduced fluorescence e

293 or, and a heterodimeric cytokine receptor in living cells with excellent sensitivity and high signal-

294 y required for specific molecular imaging of living cells with high spatial resolution.

295 urotransmitter such as noradrenaline (NA) in living cells with simple, sensitive and selective assays

296 conditions to image target molecules inside living cells with very high vibrational selectivity and

297 non-natural chemical transformations within living cells, with the highly efficient labeling of subc

298 the respiratory syncytial virus G protein in living cells without disrupting respiratory syncytial vi

299 evaluated in situ using model membranes and living cells without the need for tedious isolation and

300 donor and acceptor anti-PrP(C) antibodies to living cells yielded a measure of PrP(C) surface density