ライフサイエンスコーパス: locked nucleic acid

1 l elements of 2'-O-methoxyethyl residues and locked nucleic acid.

2 time that it is possible to obtain LNA-like (Locked Nucleic Acid 1) binding affinity and biological a

3 loops, CNG repeats, dangling ends, inosines, locked nucleic acids, 2-hydroxyadenines and azobenzenes.

4 Here, we show that antigene locked nucleic acids (agLNAs) reduce RNA levels of targe

5 nse oligonucleotides composed exclusively of locked nucleic acids (all-LNAs) complementary to CUG rep

6 on duplexes containing targeted insertion of locked nucleic acid analogs and abasic lesions in either

7 Locked nucleic acid analogs, which constrain sugar ring

8 2'-O-methyl oligonucleotide probes with LNA (locked nucleic acid) and 2,6-diaminopurine substitutions

9 (that express high levels of mir-29) with a locked-nucleic acid antagonist of mir-29b increased Mcl-

10 In the present study, we show that an 8-mer locked nucleic acid anti-miR-155 oligonucleotide targeti

11 , we observed that in vivo administration of locked nucleic acid anti-miR-181b retarded both the deve

12 d-type mice pretreated intracolonically with locked nucleic acid anti-miR-210.

13 Treatment with locked nucleic acid anti-miR-29a significantly improved

14 In vivo administration of locked nucleic acid anti-miR-29b greatly increased colla

15 ssion, but transfection with let-7f-specific locked nucleic acid anti-miRNA increased beta(2)AR expre

16 Moreover, locked nucleic acid anti-miRNA inhibitors of these six m

17 nous administration of an miR-26a inhibitor, locked nucleic acid-anti-miR-26a, increased SMAD1 expres

18 s9, or primary FLT3-ITD(+) AML samples using locked nucleic acid antisense inhibitors, results in an

19 PGDS transcript levels, the responses of the locked nucleic acid antisense oligo-treated animals from

20 We report that peptide nucleic acid and locked nucleic acid antisense oligomers that target CAG

21 ock-down of survivin using EZN-3042, a novel locked nucleic acid antisense oligonucleotide, in combin

22 Finally, miR-29b blockade by locked nucleic acid antisense oligonucleotides prevented

23 ver, in the medial amygdala of female mice, "locked nucleic acid" antisense (AS) oligonucleotides wit

24 njection of mice on a western-type diet with locked nucleic acid-antisense oligonucleotides results i

25 Conversely, locked nucleic acid-based anti-miR-34a treatment diminis

26 d a selective inhibitory PCR (siPCR) using a locked nucleic acid-based PCR blocker to selectively inh

27 Expression of miRNA was determined by locked nucleic acid-based quantitative real-time polymer

28 Oligonucleotides containing locked nucleic acid bases (LNAs) have increased affinity

29 Essentially, this new design adds locked nucleic acid bases (LNAs) to the beacon structure

30 ese results demonstrate the potential of the locked nucleic acid bases for nucleic acid design for su

31 Bis-locked nucleic acids (bisLNAs) are clamp-forming oligonu

32 Addition of an OMe G-clamp to a 12mer OMe-locked nucleic acid chimera maintained, but did not enha

33 real-time polymerase chain reaction based on locked nucleic acid-containing primers in 174 patients,

34 Pyrene-functionalized 2'-amino-alpha-L-LNAs (locked nucleic acids) display extraordinary affinity tow

35 Here, we report a double-stranded locked nucleic acid (dsLNA) biosensor for investigating

36 LNA (locked nucleic acids, i.e. oligonucleotides with a methy

37 of a subset of these genes was tested using locked nucleic acid in situ analyses.

38 Moreover, blockage of miR-30b or 378 by locked nucleic acid inhibitors significantly attenuated

39 ) and miR-221 (novel in T lymphocytes) using locked nucleic acid inhibitors.

40 cing anti-GAA duplex RNAs or single-stranded locked nucleic acids into patient-derived cells increase

41 Locked nucleic acid (LNA) and its diastereomer alpha-L-L

42 formationally restricted nucleotides such as locked nucleic acid (LNA) are very popular as affinity-,

43 To take full advantage of locked nucleic acid (LNA) based molecular beacons (LNA-M

44 Oligonucleotides that contain locked nucleic acid (LNA) bases have remarkably high aff

45 The introduction of locked nucleic acid (LNA) bases into oligonucleotides co

46 stilbene donor-acceptor capped hairpin with locked nucleic acid (LNA) bases on the dynamics and effi

47 First, locked nucleic acid (LNA) bases were incorporated into t

48 Locked Nucleic Acid (LNA) can enhance oligonucleotide af

49 ntaining bicylic modifications (BNA) such as locked nucleic acid (LNA) designed to induce target RNA

50 he (-) DNA template by inserting overlapping locked nucleic acid (LNA) doublets and triplets.

51 Oligonucleotide probes containing locked nucleic acid (LNA) hybridize to complementary sin

52 s study we show by in vivo administration of locked nucleic acid (LNA) inhibitors that suppression of

53 Locked nucleic acid (LNA) is one of the most useful modi

54 Locked nucleic acid (LNA) microarrays were used to inves

55 ning either 2'-O-methoxyethylribose (MOE) or locked nucleic acid (LNA) modifications were designed to

56 The DNA-BCA system contains a locked nucleic acid (LNA) modified DNA probe for improvi

57 that the 2'-N-(pyren-1-yl)carbonyl-2'-amino locked nucleic acid (LNA) monomer X is a highly versatil

58 entary road controlled by affinity-enhancing locked nucleic acid (LNA) monomers and additional regula

59 formationally restricted nucleotides such as locked nucleic acid (LNA) monomers are used extensively

60 nd TC-motif TFOs modified with six different locked nucleic acid (LNA) monomers, i.e. conventional an

61 tural elements of 2'O-methoxyethyl (MOE) and locked nucleic acid (LNA) nucleosides yielded a series o

62 introduction of 2'-deoxy-2'-fluorouridine or locked nucleic acid (LNA) nucleotides.

63 is, we investigate the use of functionalized locked nucleic acid (LNA) oligomers as biomolecular hand

64 hat antisense peptide nucleic acid (PNA) and locked nucleic acid (LNA) oligomers can bind key IRES se

65 In WTB-PCR, a nonextendable locked nucleic acid (LNA) oligonucleotide binds tightly

66 ocalization of small RNAs in plants by using locked nucleic acid (LNA) oligonucleotide probes.

67 wild-type BRAF is competitively blocked by a locked nucleic acid (LNA) oligonucleotide.

68 Conjugates containing DNA and locked nucleic acid (LNA) oligonucleotides are active, a

69 Transfection of locked nucleic acid (LNA) oligonucleotides designed to b

70 Optimum results were obtained with locked nucleic acid (LNA) phosphorothioate gap-mers.

71 We also demonstrated that locked nucleic acid (LNA) probes improved sensitivity ap

72 ntaining mutations at the targeted loci, and locked nucleic acid (LNA) probes were used to enhance th

73 re based on robust digoxigenin (DIG) RNA and locked nucleic acid (LNA) probes, respectively.

74 Use of locked nucleic acid (LNA) residues in the DNA probes res

75 The influence of locked nucleic acid (LNA) residues on the thermodynamic

76 nucleotide containing 7xOMe and 5x5-methyl C locked nucleic acid (LNA) residues, and a peptide nuclei

77 Locked nucleic acid (LNA), a conformationally restricted

78 e of primer chemistries comprising DNA, RNA, locked nucleic acid (LNA), or 2'O-methyl-DNA.

79 hereas inhibition of the let-7 family with a locked nucleic acid (LNA)-anti-miR has the opposite effe

80 With the use of locked nucleic acid (LNA)-antimiR-21 oligonucleotides, b

81 Locked nucleic acid (LNA)-based miRNA array was employed

82 molecular beacons with DNA, RNA and combined locked nucleic acid (LNA)-DNA backbones can all detect m

83 s two spectrally distinguishable fluorescent locked nucleic acid (LNA)-DNA oligonucleotide probes to

84 amage in porcine cardiac tissues and whether locked nucleic acid (LNA)-modified anti-miR chemistries

85 herapeutic potential of 8-mer seed-targeting locked nucleic acid (LNA)-modified anti-miR oligonucleot

86 using an s.c.-delivered seed-targeting 8-mer locked nucleic acid (LNA)-modified antimiR (LNA-antimiR-

87 gets the liver-expressed miRNA-122 using the locked nucleic acid (LNA)-modified antisense oligonucleo

88 In the present study, the locked nucleic acid (LNA)-modified cap analogue 3, m(7(L

89 We evaluated the efficacy of a locked nucleic acid (LNA)-modified inhibitor of miR-192,

90 )-ribose, or 2'-O, 4'-C-methylene bridged or locked nucleic acid (LNA)-modified nucleotides show subs

91 t of chronically infected chimpanzees with a locked nucleic acid (LNA)-modified oligonucleotide (SPC3

92 essenger RNA (mRNA) in suspended cells using locked nucleic acid (LNA)-modified oligonucleotide probe

93 ded (FFPE) tissues and cultured cells, using locked nucleic acid (LNA)-modified oligonucleotides.

94 the IL-6-deficient (IL-6((-)/(-))) mice with locked nucleic acid (LNA)-modified phosphorothioate olig

95 emonstrate efficient delivery using a simple locked nucleic acid (LNA)-polymer conjugate that assembl

96 estricted nucleotide building blocks such as locked nucleic acid (LNA).

97 DNA/locked nucleic acids (LNA) AMOs synthesized with a phosp

98 G)-labeled oligonucleotide probes containing locked nucleic acids (LNA) and 1-ethyl-3-(3-dimethylamin

99 combination of site-directed mutagenesis and locked nucleic acids (LNA) complementary to defined doma

100 ation of TD05 coupled with modification with locked nucleic acids (LNA) increased conformational stab

101 Locked nucleic acids (LNA) show remarkable affinity and

102 We have used locked nucleic acids (LNA) to engineer novel molecular b

103 that Hsp90 protein binds PS-ASOs containing locked-nucleic-acid (LNA) or constrained-ethyl-bicyclic-

104 Inhibition of miR-30 using complementary locked nucleic acids (LNA30bcd) in both human IECs and h

105 However, the recent additions to the field - locked nucleic acids (LNAs) and peptide nucleic acids (P

106 Locked nucleic acids (LNAs) are synthetic nucleic acid a

107 e the potential of novel antigens containing locked nucleic acids (LNAs) as targets for antibodies.

108 linkages and also 2'-OMe ribonucleotides and locked nucleic acids (LNAs) at selected sites.

109 Here, we describe a unique use of locked nucleic acids (LNAs) for studying nuclear long no

110 ioate antisense oligonucleotides (ASOs) with locked nucleic acids (LNAs) improve target affinity, RNa

111 The presence of locked nucleic acids (LNAs) in the ssODNs at mismatching

112 Therapeutic delivery of locked nucleic acids (LNAs) targeting BCAR4 attenuated Y

113 breast cancers, and therapeutic delivery of locked nucleic acids (LNAs) targeting BCAR4 strongly sup

114 Treatment of cells with locked nucleic acids (LNAs) targeting these miRNAs inhib

115 bility of oligonucleotides (ODNs) containing locked nucleic acids (LNAs) to bind supercoiled, double-

116 In 2005, we introduced Invader locked nucleic acids (LNAs), i.e., double-stranded probe

117 d gain-of-function studies, using anti-sense locked-nucleic acids (LNAs) and synthetic RNA mimetics,

118 led miRNAs by next generation sequencing and locked nucleic acid miRNA microarrays and verified conco

119 The strong northern-biased locked-nucleic acid modification exacerbated the negativ

120 of miR-21 through intravenous delivery of a locked nucleic acid-modified (LNA-modified) antimiR olig

121 Blocking endogenous miR-140 by locked nucleic acid-modified anti-miR partially sensitiz

122 A locked nucleic acid-modified anti-miR-146a given before

123 Inhibition of miR-23 and miR-27 function by locked nucleic acid-modified anti-miRNAs represses angio

124 n of the miR-15 family in neonatal mice with locked nucleic acid-modified anti-miRNAs was associated

125 iac remodeling, our current study employed a locked nucleic acid-modified antimiR to target miR-378 i

126 Locked nucleic acid-modified antisense miR-92a (LNA-92a)

127 Here, we have shown that a locked nucleic acid-modified antisense oligonucleotide c

128 cardiac mRNA for therapeutic purposes using locked nucleic acid-modified antisense oligonucleotides.

129 ction, and extracellular matrix genes, using locked nucleic acid-modified antisense TFIIB oligonucleo

130 Miravirsen is a locked nucleic acid-modified DNA phosphorothioate antise

131 Targeting miR-192 with locked nucleic acid-modified inhibitors in vivo decrease

132 diac disease models were administered with a locked nucleic acid-modified oligonucleotide (LNA-antimi

133 The simple systemic delivery of locked nucleic acid-modified, anti-miR-29b-reduced endog

134 miR-21 inhibition using locked-nucleic-acid-modified oligonucleotide effectively

135 isense oligonucleotides (oligos), containing locked nucleic acid moieties (an improved technology), t

136 A gold nanorod-locked nucleic acid nano-biosensor for dynamic single-ce

137 In situ hybridization analysis, using locked nucleic acid oligo probes complementary to the n1

138 ibers, we blocked miR-210 with complementary locked nucleic acid oligonucleotides (anti-miR-210).

139 bilateral intraamygdala injections of OTR AS locked nucleic acid oligonucleotides several days before

140 ymine, is weakly hybridized to complementary locked nucleic acid or 2'-OMe RNA ONs that are also subs

141 2', 4'-bridged nucleic acids (also known as locked nucleic acid or LNA, and 2',4'-constrained ethyl

142 Blocking miR-146a with locked nucleic acids or antago-miRs attenuated PPCM in C

143 properties were optimized using 2'-O-methyl, locked nucleic acid, or phosphorodiamidate morpholino ba

144 Using a locked nucleic acid primer specific for > or =6 AT repea

145 paraffin-embedded tissues were hybridized to locked nucleic acid probes against 752 human miRNAs (rep

146 ned optimal hybridization parameters for 130 locked nucleic acid probes by recording nucleic acid mel

147 We used locked nucleic acid probes for in situ hybridization ana

148 We used locked nucleic acid probes in in situ hybridisation reac

149 ombines the unique recognition properties of locked nucleic acid probes with enzyme-labeled fluoresce

150 Using hybridization with locked nucleic acid probes, we directly detected abortiv

151 As is achieved by in situ hybridization with locked nucleic acid probes.

152 cent in situ hybridization (flow-FISH) using locked-nucleic acid probes is combined with rolling circ

153 ilencing of miR-125b by systemic delivery of locked nucleic acid rescued angiotensin II-induced periv

154 T(L)AC(L)GA(L)CG(L)GC(L)C, with L denoting a locked nucleic acid residue, inhibit 50% of group I intr

155 Oligonucleotides composed of 2'-O-methyl and locked nucleic acid residues complementary to HIV-1 tran

156 ice, inhibition of miR-193a by complementary locked nucleic acids resulted in an upregulation of the

157 Control of ground-state conformation by the locked nucleic acids results in a marked increase in bot

158 inhibition of miR-30a-5p in the mPFC using a Locked Nucleic Acid sequence that targets miR-30a-5p res

159 Purification protocols using oligo dT's, locked nucleic acid substituted dT's, and tetramethylamm

160 l agRNAs can contain 2'F-RNA, 2'OMe-RNA, and locked nucleic acid substitutions, or combinations of mu

161 Consistent with this notion, let-7f locked nucleic acid transfection caused depressed agonis

162 nd duplex DNA structures possessing a single locked nucleic acid TT step are reported.