ライフサイエンスコーパス: long-term culture

1 differentiated state for more than 30 days (long-term culture).

2 the absence of a specific gene when grown in long term culture.

3 sc composition, structure, and function with long-term culture.

4 unable control over cell-contact time during long-term culture.

5 as generally been problematic, especially in long-term culture.

6 r also exhibited homogeneous inducibility in long-term culture.

7 mortalizes human CD34(+) cord blood cells in long-term culture.

8 tinued to express CD34 throughout the 5-week long-term culture.

9 erminal differentiation in vitro, even after long-term culture.

10 rvived longer than wild-type spleen cells in long-term culture.

11 fferentiation of cord blood CD34(+) cells in long-term culture.

12 and assess adenoviral-associated toxicity in long-term culture.

13 tained from a single mouse and maintained in long-term culture.

14 CD34(+) and CD34(+)38(-) clonogenic cells in long-term culture.

15 was statistically associated with successful long-term culture.

16 ary cell population that can be amplified in long-term culture.

17 ting clones, 17A and 17B, were maintained in long-term culture.

18 and its deficiency promoted HSC expansion in long-term culture.

19 ibution is not altered by immortalization or long-term culture.

20 oblast differentiation and mineralization in long-term culture.

21 llagen matrix persisted and increased during long-term culture.

22 r/tibia HSCs, including clonogenic assay and long-term culture.

23 aSCs (cMaSCs) lose their growth potential in long term cultures.

24 on and increased survival in both short- and long-term cultures.

25 e survival of HIV-specific CD8(+) T cells in long-term cultures.

26 are fully functional and equally expanded in long-term cultures.

27 ansfer using embryonic fibroblast cells from long-term cultures.

28 Bovine CECs were grown and aged as long-term cultures.

29 regulatory T cells that can be maintained in long-term cultures.

30 the glucocorticoid induction of apoptosis in long-term cultures.

31 virus serotype 16 was necessary to establish long-term cultures.

32 cursors for colony-forming cells detected in long-term cultures.

33 e emergence of SCH772984-resistant clones in long-term cultures.

34 ys, which require large numbers of cells and long-term cultures.

35 ormed, cultured human corneal fibroblasts in long-term cultures.

36 o be examined in hPSC cardiomyocytes include long-term culturing, 3-dimensional tissue engineering, m

37 fferentiated into cardiomyocytes, even after long-term culture (50 passages or approximately 260 popu

38 Our method allows long-term culture (~50 days, 10 passages tested, accumul

39 Long-term cultures (72 h postinfection) of infected phag

40 ector was seen for over 15 weeks of extended long-term culture (9.2 +/- 5.2%, n = 7).

41 These data show that under long-term culture adult S. neumayeri appear to acclimate

42 topoietic progenitors detected in short- and long-term culture analyses.

43 utant iPSC-MNs display decreased survival in long-term culture and exhibit hnRNP A2/B1 localization t

44 o establish a cell line that is suitable for long-term culture and large-scale in vitro experimentati

45 ented a microfluidic bioreactor that enables long-term culture and monitoring of extremely small popu

46 itive hematopoietic potential as measured by long-term culture and phenotypic analysis.

47 functional islet tissue can be maintained in long-term culture and successfully transplanted.

48 nephron stem cell niche and hold promise for long-term culture and utilization of these progenitors i

49 e previously defined using NK cell clones in long-term culture and with the frequencies of cells expr

50 cells derived from marrow and cord blood in long-term cultures and long-term culture-initiating cell

51 te and maintain leukemic cell growth in both long-term cultures and nonobese diabetic/severe combined

52 when the primary lymphomas were subjected to long-term culture, and completely missed in the standard

53 ndifferentiated, pluripotent ES cells during long-term culture, and maintain their potential to diffe

54 e is designed to maintain cell viability for long-term culture as well as to introduce exogenous reag

55 ome, produced very low levels of virus after long-term culture, as previously reported in other astro

56 NOD/SCID) mice, and in primary and secondary long-term culture assays.

57 phases do not separate from each other) over long-term culture (at least 3 weeks) and support spatial

58 In these long-term cultures, bone marrow microvascular endothelia

59 creases in parallel with the loss of CD28 in long-term cultures, but this effect is blunted in the pr

60 ted beta-catenin expands the pool of HSCs in long-term cultures by both phenotype and function.

61 analysis at the individual cell level during long-term culture, by FISH techniques, reveals chromosom

62 However, reprogramming and long-term culture can also induce abnormalities in these

63 whether adult self-renewing spermatogonia in long-term culture can generate such stem cells as well.

64 d for in vitro stem-cell-like multipotential long-term culture capability.

65 Long-term cultured cells of this kind retained some pote

66 In addition to experiments on long-term cultured cells, we also found that wild-type p

67 were seen in cultured chondrocytes, a stable long-term culture chondrosarcoma cell line, as well as C

68 s) and more primitive stem/progenitor cells (long-term culture colony-forming cells) could be shown a

69 32D/EGFRvIII P5 cells subjected to long-term culture conditions in the absence of IL-3 reve

70 uman hepatocyte spheroids after 3-4 weeks of long-term culture confirmed the presence of the liver-sp

71 onsmoker and smoker control subjects, during long-term culture, COPD fibroblasts displayed increased

72 ls was sustainable yet reversible even after long-term culture, demonstrating the impact of mechanica

73 Long-term culture did not affect the chondrogenic potent

74 CLiPs in long-term culture did not lose their proliferative capac

75 Long-term cultures displayed a myelomonocytic morphology

76 o test somatic CAG-repeat alterations during long-term culture, DNA was extracted from transgenic tis

77 )KDR(+) cells were grown in 3-month extended long-term culture (ELTC) through 3 serial culture rounds

78 these iTreg displayed a stable phenotype in long-term cultures, even in the presence of proinflammat

79 ary mouse hepatocytes that also show GJIC in long-term culture for 30 days.

80 cells capable of sustaining hematopoiesis in long-term cultures for 5 weeks.

81 ststimulation, and virus was recovered after long-term culture from a macrophage expressing dendritic

82 Long-term cultures (> 50 population doublings [PDs]) wer

83 ator to maintain homogeneous inducibility in long-term culture has not been examined.

84 In the first stage (human B-progenitor long-term culture [HB-LTC]), CD34(+) hematopoietic proge

85 ells using retroviral vectors from short- or long-term cultured human and mouse amniocytes using four

86 Interestingly, long-term-cultured human epithelial cancer cells in clon

87 uccessful in maintaining trabecular cells in long-term culture if low perfusion rates occur.

88 h were investigated in airway epithelia in a long term culture in the absence of luminal infection, w

89 ability effect of the agonist was blocked by long-term culture in 25 mM glucose.

90 bited synaptic transmission between SCGNs in long-term culture in a time-dependent manner, significan

91 Many minichromosomes were lost after long-term culture in the absence of telomerase, which ma

92 can be stably maintained during continuous, long-term culture in the presence of drug selection.

93 However, resistant clones occur upon long-term culture in the presence of inhibitors.

94 ered tissues, allowing for ease of handling, long-term culture in vitro and anchoring of the central

95 ic progenitor cells remain pluripotent after long-term culture in vitro and that E2A proteins play a

96 they may undergo progressive adaptation upon long-term culture in vitro.

97 ype 1 (HIV-1) is frequently attenuated after long-term culture in vitro.

98 21 patients with MDS was then propagated in long-term cultures in the presence or absence of TRAIL.

99 6-infected primary cell lines established as long-term cultures in vitro was also evaluated at RNA an

100 Our data demonstrates that extensive long-term culture-induced MSC aging impaired their osteo

101 ells to Pseudomonas aeruginosa obtained from long-term cultures inhibits Duox1-dependent hydrogen per

102 We have recently reported that long-term cultures initiated with CD34+CD38- cells from

103 effect on CFU-GM and BFU-E formulation or on long term culture initiating cells.

104 e LTC-IC period (35 to 60 days) and extended long-term culture initiating cell (ELTC-IC) period ( > 6

105 following chemotherapy and a higher leukemic long-term culture initiating cell potential, targeting m

106 We have demonstrated that long-term culture initiating cells (LTC-IC) are maintain

107 in -/34(+)/DRdim cells could generate 1 to 3 long-term culture initiating cells (LTC-IC) as well as 1

108 We have recently shown that more than 90% of long-term culture initiating cells (LTC-IC) mobilized in

109 -forming unit-granulocyte-macrophage and for long-term culture initiating cells as compared with bone

110 lineage(-) and Sca(+) lineage(-) cells, and long-term culture initiating cells.

111 ells from patients with AML were enriched in long-term culture, initiating cells and repopulating cel

112 ormation from purified CD34+ marrow cells in long term culture-initiating cell assays.

113 e IGF-II-dependent enhancement of CFU-GM and long term culture-initiating cell numbers.

114 ophages (CFU-GM), and primitive progenitors, long term culture-initiating cells (LTC-IC) derived from

115 Long-term culture-initiating assays with CD34(+)/CD38(-)

116 not yet routine, in vitro assays such as the long-term culture-initiating cell (LTC-IC) assay have be

117 that IFN-gamma is a potent inhibitor in the long-term culture-initiating cell (LTC-IC) assay, the be

118 row and cord blood in long-term cultures and long-term culture-initiating cell (LTC-IC) assays.

119 ration on days 3, 5, and 7, then assayed for long-term culture-initiating cell (LTC-IC) function on d

120 Long-term culture-initiating cell (LTC-IC) maintenance w

121 omposed mostly of progenitors and cells with long-term culture-initiating cell (LTC-IC) potential.

122 with acquired aplastic anemia (AA) using the long-term culture-initiating cell assay (LTC-IC), in par

123 mitive leukemic cells capable of growth in a long-term culture-initiating cell assay and expansion on

124 The inclusion of BCCs in stromal support of long-term culture-initiating cell assay frequencies show

125 Long-term culture-initiating cell assays demonstrated a

126 Long-term culture-initiating cell assays on murine strom

127 Of relevance is the novel finding by long-term culture-initiating cell assays that showed an

128 The clone detected in the long-term culture-initiating cell compartment that recon

129 ut not CD34(+)ACE(-) cells, are endowed with long-term culture-initiating cell potential and sustain

130 y of FL and SCF to maintain the viability of long-term culture-initiating cells (25 and 32%, respecti

131 a 5 to 7 times higher frequency of leukemic long-term culture-initiating cells (L-LTC-IC) compared w

132 Long-term culture-initiating cells (LTC-IC) are arguably

133 Long-term culture-initiating cells (LTC-IC) are hematopo

134 We now show that long-term culture-initiating cells (LTC-IC) are maintain

135 tly demonstrated that 50% of primitive human long-term culture-initiating cells (LTC-IC) are maintain

136 phenotype of primitive progenitors, such as long-term culture-initiating cells (LTC-IC) in mobilized

137 elphia chromosome (Ph)- and BCR/ABL-negative long-term culture-initiating cells (LTC-IC) in selected

138 ain-specific differences in the frequency of long-term culture-initiating cells (LTC-IC) in the bone

139 progenitors as well the ability to maintain long-term culture-initiating cells (LTC-IC) in vitro.

140 Efficient gene transfer to progenitors and long-term culture-initiating cells (LTC-IC) was obtained

141 y 12 colony-forming units-spleen (CFU-S), or long-term culture-initiating cells (LTC-IC), suggesting

142 orming unit (CFU-S) assay and in vitro using long-term culture-initiating cells (LTC-ICs) and methylc

143 f cobblestone area-forming cells (CAFCs) and long-term culture-initiating cells (LTC-ICs) in a transw

144 ced survival, serial replating capacity, and long-term culture-initiating cells (LTC-ICs) in LSCs fro

145 was not significantly altered, the number of long-term culture-initiating cells (LTC-ICs) was 1.5-fol

146 Furthermore, the number of long-term culture-initiating cells (LTC-ICs) within an a

147 that rhesus SP cells are highly enriched for long-term culture-initiating cells (LTC-ICs), an indicat

148 s (primary colony-forming cells [CFCs]), and long-term culture-initiating cells (LTC-ICs; a stem cell

149 Quantitative analysis of long-term culture-initiating cells (LTCIC) failed to dem

150 m immunophenotypic data, the effect of FL on long-term culture-initiating cells (LTCIC) was determine

151 etermine the effect of ANK on more primitive long-term culture-initiating cells (LTCIC), the IL-2-sup

152 R/ABL-positive primitive progenitors (6-week long-term culture-initiating cells [LTCICs]) as well as

153 Ph-negative long-term culture-initiating cells are detectable in man

154 ntly suppressed CML colony-forming cells and long-term culture-initiating cells but did not significa

155 hB4 depleted primitive cells, as measured by long-term culture-initiating cells or CD34(+)CD38(-) cel

156 er numbers of human colony-forming units and long-term culture-initiating cells per engrafted human C

157 Analysis of bcr/abl expression in long-term culture-initiating cells suggested that purgin

158 In contrast, even the frequency of long-term culture-initiating cells within the CD34(+) DC

159 le to generate CFU beyond 60 days ("extended long-term culture-initiating cells" or ELTC-IC) are func

160 e-macrophage colony-forming units) and late (long-term culture-initiating cells) hematopoietic progen

161 colony-forming units in methylcellulose, and long-term culture-initiating cells) occurred several day

162 l blood was not associated with detection of long-term culture-initiating cells, consistent with the

163 dition, Tpo promoted viability of CD34+CD38- long-term culture-initiating cells, further supporting t

164 med by a dramatic increase in the numbers of long-term culture-initiating cells, the most primitive h

165 ransduction of both colony-forming units and long-term culture-initiating cells, with transduction ef

166 the notion that SRCs are more primitive than long-term culture-initiating cells.

167 ia species, Plasmodium falciparum, for which long term culture is possible, and Plasmodium vivax, for

168 possible, and Plasmodium vivax, for which no long-term culture is feasible.

169 layers, the use of classic stroma-dependent long-term cultures is not possible.

170 We evaluated the use of long-term cultured islet cells for the treatment of diab

171 CR and CNR cells were cultured in secondary long-term cultures (LTCs) and assayed weekly for transdu

172 Taken together, our results demonstrate that long-term cultures maintained on multi-electrode arrays

173 h stromal ligands, hydrocortisone-containing long-term culture medium, IL-2, IL-7, and stem cell fact

174 ns poorly known, mainly due to the lack of a long-term culture method for this parasite.

175 bstrate-integrated microelectrode arrays and long-term cultured neuronal networks.

176 Long term culture of MCF-7 cells in estrogen (E2)-deplet

177 As expected, telomerase activity declined in long term culture of stable transfectants.

178 vage of fibrocystin occurs constitutively in long term cultures of polarized inner medullary collecti

179 Short term and long term cultures of transfected HEK293 cells suggest t

180 Here, we show that long term culturing of cells in the presence of the LRP-

181 Interestingly, long-term culture of a virus lacking the BHA cytoplasmic

182 e plated under Whitlock-Witte conditions for long-term culture of B lineage cells.

183 Long-term culture of B-CLL clones would permit the colle

184 stem cell properties can be derived from the long-term culture of diverse tissues, it is not clear wh

185 Long-term culture of EBV+ lymphoblastoid cells in IFN-al

186 Long-term culture of EIAV-transduced human cells showed

187 Finally, via long-term culture of gastric tumour epithelium, we revea

188 xpansion within the mutant HTT allele during long-term culture of HD cells.

189 Long-term culture of infected Huh7.5 cells with increasi

190 Our results show that SFM allows for the long-term culture of islet tissue.

191 We show that CDF can support the long-term culture of laboratory strains and demonstrate

192 In the present study, a long-term culture of M. avium-M. intracellulare-infected

193 vaccine is the only option in the absence of long-term culture of P. vivax parasites.

194 We used short-term and long-term culture of pal-mutated viruses in permissive C

195 nize cell-surface glycans can facilitate the long-term culture of pluripotent stem cells.

196 Long-term culture of primary human cells modified with e

197 been limited by the lack of methods for the long-term culture of primary human intestinal epithelial

198 Long-term culture of primary murine small intestinal epi

199 We describe a method for long-term culture of primary small intestinal epithelial

200 g a 3D organoid system, we report success in long-term culture of prostate cancer from biopsy specime

201 the EBV P3HR-1 strain, we have reproduced in long-term culture of SVK epithelial cells an unusual pat

202 Long-term culture of transfectant clones in the absence

203 In long-term culture of transfected MDA-MB-231 cells, expre

204 However, it has been difficult to establish long-term cultures of adenoma cells, especially those of

205 F74 did not detect viral gene transcripts in long-term cultures of bone marrow stromal cells from 23

206 signals in the cortical regions, short- and long-term cultures of E14.5 telencephalic progenitors we

207 Long-term cultures of GPR125+ SPCs (GSPCs) also converte

208 developmental progression, we have generated long-term cultures of hematopoietic progenitors by enfor

209 show that modulation of TNF-alpha levels in long-term cultures of human CD8(+) T lymphocytes, by chr

210 t the establishment, from mouse ES cells, of long-term cultures of immature DCs that share many chara

211 Sequence analysis of viral DNA derived from long-term cultures of Jurkat cells revealed a specific m

212 s observed in conditioned media derived from long-term cultures of mouse Lewis lung carcinoma cells M

213 es containing conditioned media derived from long-term cultures of mouse Lewis lung carcinoma cells w

214 ecruitment to conditioned media derived from long-term cultures of mouse Lewis lung carcinoma cells.

215 virus (HCV) infection require examination of long-term cultures of normally differentiating primary h

216 Treatment with NGF causes long-term cultures of oligodendrocytes to die via a yet

217 Long-term cultures of SV40-infected human keratinocytes

218 Long-term cultures of T cells and FLS form heterotypic f

219 ve protein (PARP) fraction was purified from long-term cultures of Trypanosoma brucei procyclic forms

220 Long-term cultures of unstimulated mouse HSCs secreted I

221 We report the derivation of long-term cultures of untransformed DCs, uniformly expre

222 day 14, and expression of IL-10 continued in long-term cultures of up to 120 days.

223 ould make them useful laboratory models, but long-term culturing of tardigrades historically has been

224 lly isolated from the relevant T cells after long-term culture, often after repeated antigen stimulat

225 y acquire the ability to form colonies after long-term culture on bone marrow stroma, coincident with

226 ng cell (CAFC) subsets in stromal-associated long-term cultures on fresh and frozen PBPC.

227 Porcine hematopoiesis can be maintained in long-term cultures on primate stroma with pig cytokines.

228 a limited fashion without measurable loss of long-term culture or in vivo engrafting potential as mea

229 freshly isolated, short term activated, and long term cultured PBT.

230 theca cells from PCOS ovaries maintained in long term culture persistently secrete significantly gre

231 s (apoptotic indices) between the short- and long-term culture-positive animals were not different.

232 t study, we showed that, although a panel of long-term cultured rat uveitogenic T cell lines specific

233 ted to a small number of lineages, and their long-term culturing remains problematic.

234 We conclude that long-term cultured rhesus macaque spleen-derived Valpha2

235 Progenitor cells from long-term cultures showed altered expression of genes de

236 lretinin and bromodeoxyuridine antibodies in long-term cultures showed that only a few mitotic utricu

237 IHK-beta-globin transgenes from silencing in long-term culture studies.

238 n a newly developed, stromal cell-dependent, long term culture system, the ability of selected thymic

239 Furthermore, development of a long-term culture system for rat SSCs has established a

240 We evaluated HCV-IFN interactions within a long-term culture system of Huh7 cell lines harboring di

241 Long-term culture systems have played a pivotal role in

242 early lethality of the Tbx1-/- mice, we used long-term culture techniques that allow the unharmed gro

243 ansgene were observed in cells maintained in long-term culture that had been infected with the LA vec

244 In MS-5 feeder cell-based long-term cultures that supported the growth of primary

245 When maintained in long term-culture, the L2198S RNA evolved into a stable

246 al endothelial cells which remain diploid in long-term culture, the aneuploidy of tumor endothelial c

247 In long-term cultures, the granular layer appeared well pre

248 D34+ HLA-DR+ mobilized PB cells can initiate long-term cultures, they are relatively mature and canno

249 activity in vivo and maintain the ability in long-term culture to give rise to multipotent adult sper

250 e the requirement for in vitro activation or long-term culture to introduce the transgene and obtain

251 d used marrow-derived preadipocyte lines and long-term cultures to explore potential roles in hematop

252 In long-term cultures, typical cellular morphology and pigm

253 This study examined the effects of long-term culture under altered conditions on the Antarc

254 ighly oligotrophic conditions which disabled long-term culturing under laboratory conditions.

255 tilage biopsies of patients and subjected to long-term culture undergo dedifferentiation and that the

256 Normal dog gallbladder epithelial cells in long-term culture were used as a model to study the morp

257 Four long-term cultures were established from 39 attempts.

258 city of T cells from fresh leukocytes and of long-term cultures were monitored by flow cytometry.

259 exhibit enhanced proliferative responses in long-term cultures when stimulated to divide with antibo

260 prediabetic adult non-obese diabetic mice in long-term cultures, where they were induced to produce f

261 is a functional hierarchy of progenitors in long-term culture which correlates with their level of q

262 al respiratory rate and enzyme activities by long-term culture with 2 mmol/L adenosine 5'-diphosphate

263 king the p53-inactivating domain to maintain long-term cultures with a p53-responsive phenotype.

264 ially induce migration in Th1 cells, and, in long-term cultures with IL-2, IL-16 facilitates the expa

265 3 dimensional growth in agarose gels and in long-term cultures within matrigel.