ライフサイエンスコーパス: low-affinity binding

1 h affinity binding while having no effect on low affinity binding.

2 Transfection of TSLPR cDNA resulted in only low affinity binding.

3 a second estimate of 1.4 microM was made for low-affinity binding.

4 there a phenotype-specific downregulation of low-affinity binding.

5 stroyed norepinephrine neurons and abolished low-affinity binding.

6 ous dopamine neurons and selectivity ablated low-affinity binding.

7 eural agrin to intact myotubes revealed only low-affinity binding.

8 unts for matrix:cell volume ratio, high- and low-affinity binding, activity coefficients, background

9 Since alpha(1)-antagonists display low affinity binding at beta(2)-adrenergic receptors, tw

10 g a possible mechanism whereby specific, yet low-affinity, binding between Nup98 and Nup96 is respons

11 Finally, destabilization of low affinity binding by salt is in quantitative agreemen

12 In addition, stabilization of the low affinity binding conformation of the C2 domain of fV

13 r between a QD and a molecular probe to even low-affinity binding events at the QD/solvent interface.

14 These technologies must be able to identify low-affinity binding events that are often observed betw

15 One subunit, IFNAR1, has low affinity binding for interferon yet is required for

16 he drug binds are unknown, although high and low affinity binding has been reported in cardiomyocytes

17 inished nicotine-induced increased nicotinic low-affinity binding in the cerebellum and diminished di

18 potentiating effect of increasing nicotinic low-affinity binding in the frontal cortex.

19 shed dizocilpine-induced increased nicotinic low-affinity binding in the hippocampus and hypothalamus

20 veloped DIO after 3 months on HE diet, their low affinity binding increased slightly.

21 The low affinity binding is competed by nonlytic human HDL a

22 g is destabilized by osmotic stress, whereas low affinity binding is insensitive.

23 mediated by Lys(415), whereas the source of low affinity binding is unknown.

24 domain 3 alone construct is capable of only low affinity binding (K(d) approximately 500 nm) toward

25 the theory predicts that both catalytic and low-affinity binding (K(d) greater, >or= 0(-7) M) protei

26 alone resulted in a protein capable of only low affinity binding (Kd approximately 1 microm) toward

27 Addition of low-affinity binding ligands had no detectable effect on

28 cell interactions could be characterized by low-affinity binding (< or = 10(5) M(-1)) to a high numb

29 inity ADP binding mode (K(app1)) but not the low affinity binding mode (K(app2)).

30 e substrate, whereas MDDMA and MDTMA adopt a low-affinity binding mode consistent with an inhibitor,

31 ional selection mechanism contrasts with the low-affinity binding mode of 53BP1, and it ensures 53BP1

32 RovA contacts mainly the DNA backbone in a low-affinity binding mode, which allows the immediate re

33 The number of low-affinity binding motifs is significantly depressed i

34 is is achieved using multiple, minimalistic, low-affinity binding motifs that are in rapid exchange w

35 25I-EGF, the EGFR/RET chimera exhibited only low affinity binding of 125I-EGF.

36 HV-cyclin complexes in vitro indicating that low affinity binding of cyclins to the Cdk subunit might

37 These effects were mediated by direct low affinity binding of estradiol to the maxi-K channel

38 tch regions of CDC42 or RAC1 and (ii) a very low affinity binding of GRD and a C terminus adjacent to

39 the mutation minimally affected the initial low affinity binding of heparin to antithrombin, but gre

40 bdominance of the JHA 210-219 was not due to low affinity binding of JHA 210-219 to H-2Kd or to ineff

41 By contrast with TPA, low affinity binding of SAPD was inhibited by sn-1,2-dio

42 f ATP (K(d)(ATP)=5-6 microM) was followed by low affinity binding of Tide (K(d)(Tide)=180 microM), an

43 T) proteins, GSTA1 and GSTM1, exhibited only low affinity binding of xanthophylls.

44 h DnaB protein, and in unwinding of oriC, or low-affinity binding of ATP.

45 higher concentrations, which may rely on the low-affinity binding of CNTF to the IL-6R.

46 al structural changes necessary to transform low-affinity binding of dofetilide by the related bovine

47 charge is followed by the voltage-dependent low-affinity binding of extracellular chloride ions.

48 ith the allosteric binding site manifests as low-affinity binding of methoctramine at the M(3) recept

49 litate plasma membrane targeting through the low-affinity binding of NB to phosphorylated inositol po

50 c18/SNARE interaction modes involve the same low-affinity binding of the extreme syntaxin-1 N terminu

51 the context of the holenzyme, and we detect low-affinity binding of the kinase and dsRBD constructs

52 (human numbering), which is responsible for low-affinity binding of the substrate in endo-mode.

53 PF recognition, thus stabilizing networks of low affinity binding partners at endocytic sites.

54 In contrast, complexes made with low affinity binding peptides were highly sensitive to d

55 Only low-affinity binding (pKI) was observed when guanine nuc

56 own to be regulated by two discrete high and low affinity binding regions for diacylglycerols and pho

57 isotherms exhibit apparent high-affinity and low-affinity binding regions, corresponding respectively

58 dentified to date contain almost exclusively low affinity binding sequences, our analysis indicates t

59 brin sequence is an important constituent of low affinity binding, since thrombin binding at this sit

60 binding site (Ki = 50 microM) and a second, low affinity binding site (Kii = 0.7 mM).

61 and AP2gamma resulted in the activation of a low affinity binding site construct to levels comparable

62 In this study, both a high affinity and a low affinity binding site for omega-conotoxin MVIIC were

63 ereas the phosphorylated Thr(187) provides a low affinity binding site for the Skp2/Cks1 complex.

64 constant of approximately 10(7) M-1 and the low affinity binding site has an apparent binding consta

65 ovide a structural basis for the role of the low affinity binding site in FGF receptor discrimination

66 These results indicate a second low affinity binding site in the Robo-Slit complex as we

67 The low affinity binding site is shown to be present on the

68 gand stacking are consistent with the single low affinity binding site observed for 4-CPI and 4-PP.

69 histamine differs significantly between the low affinity binding site of FS-HBP and monomine, sugges

70 e, mediated by an LXCXE motif and a separate low affinity binding site present within an activation d

71 ted that contained a high affinity G-rich or low affinity binding site.

72 g site and a 27% increase in the Bmax of the low affinity binding site.

73 D(2) and A(2A) receptors are involved in the low affinity binding site.

74 ve a value that allows interactions with the low affinity binding site.

75 Soluble Hb bound saturably to a low-affinity binding site [K(d) = (1.1+/-0.2) x 10(-6) M

76 bition can be explained by the presence of a low-affinity binding site and in vivo hydrolysis of NPA.

77 extends upstream, another overlaps the ToxT low-affinity binding site around the -35 element, and th

78 omers, one of which possessed a promiscuous, low-affinity binding site for aromatic ligands, includin

79 2, PDE5, and PDE6, which likely constitute a low-affinity binding site for cGMP.

80 This sequence contains a low-affinity binding site for POU domain proteins, and c

81 Further evidence for a low-affinity binding site is indicated by the inhibitory

82 The association constant for this low-affinity binding site of DtxR(E175K) obtained from c

83 The single low-affinity binding site of the K324A mutant to isoprot

84 2)PKG-I beta requires binding of cGMP to the low-affinity binding site of the R domain.

85 while binding of calmodulin to the supposed low-affinity binding site on CaMKII is compatible with c

86 lity of the Katp channel suggesting that the low-affinity binding site resides on these neurons.

87 The KD for the low-affinity binding site was determined to be 1.1 micro

88 A high-affinity (0.6 x 10(-9) mmol/L) and a low-affinity binding site were present.

89 of 2 x 10(-7), binding site 2 (primary) is a low-affinity binding site with a binding constant of 6.3

90 n are mediated respectively by the high- and low-affinity binding site(s) of IKP104.

91 Interestingly, an additional low-affinity binding site, mediated by a structurally an

92 pin transition, thus revealing an additional low-affinity binding site.

93 ical interactive high-affinity sites and one low-affinity binding site.

94 giotensin IV) competed with both a high- and low-affinity binding site.

95 caused a 70-80% reduction in its binding to low affinity binding sites establishing its identity as

96 eas the EGF receptor exhibited both high and low affinity binding sites for 125I-EGF, the EGFR/RET ch

97 Relatively low affinity binding sites for monosaccharides are forme

98 >90% of TFPI bound to a single population of low affinity binding sites on hepatoma cells (2 x 10(6)

99 difference being due to the contribution of low affinity binding sites on the RRE.

100 But DIO-prone rats had few or no low affinity binding sites throughout the forebrain.

101 unt of weight as chow-fed controls but their low affinity binding sites were reduced to DIO levels an

102 inding sites, but also numerous moderate and low affinity binding sites, are under negative selection

103 ionic channel activity at the phospholipids low affinity binding sites, thus indicating that SecA is

104 he overall enhancer architecture-clusters of low affinity binding sites-is maintained and required fo

105 one high affinity binding site and multiple low affinity binding sites.

106 eoglycans (HSPGs) may be responsible for the low affinity binding sites.

107 ns with Trk A also resulted in predominantly low affinity binding sites.

108 nding domain and its complexes with high and low affinity binding sites.

109 bind approximately 1.6 thrombin molecules at low-affinity binding sites (Kd = 2.8 muM) and approximat

110 igh-affinity (mAb 247) or both the high- and low-affinity binding sites (mAb 370 and mAb 387).

111 helial cells by enhancing binding of VEGF to low-affinity binding sites and neuropilin-1 and stimulat

112 conversion of CVB that include the high- and low-affinity binding sites associated with capsid breath

113 he relative proportions of high-affinity and low-affinity binding sites did not change.

114 nt receptors that had GTP-insensitive single low-affinity binding sites for agonists and reduced pote

115 We conclude that band 4.2 provides low-affinity binding sites for both band 3 oligomers and

116 ibited one high-affinity binding and several low-affinity binding sites for cis-parinaroyl-CoA, a nat

117 r, we proposed that there may be high- and a low-affinity binding sites for the BvgA dimer.

118 r the long-standing observation of high- and low-affinity binding sites for the prototypic inhibitor

119 cription factor Ultrabithorax (Ubx) utilizes low-affinity binding sites in the Drosophila melanogaste

120 ast, ADP occupies one high-affinity and five low-affinity binding sites in vitro, consistent with con

121 endent sites with the apparent population of low-affinity binding sites increasing with the size of t

122 d state are consistent with its occupancy of low-affinity binding sites necessary for promoter functi

123 ation could be due to release of band 3 from low-affinity binding sites on ankyrin.

124 iso-1-cytochrome c (yCc) with the high- and low-affinity binding sites on cytochrome c peroxidase co

125 ing studies suggest coexistence of high- and low-affinity binding sites or negative cooperativity.

126 pM and 8 nM, corresponding to the high- and low-affinity binding sites seen on phytohemagglutinin-ac

127 ochord enhancers, including those containing low-affinity binding sites that would be excluded by sta

128 into the brain parenchyma, where it bypassed low-affinity binding sites that would otherwise sequeste

129 r Irf4 abundance with its recruitment toward low-affinity binding sites within Teff cell cis-regulato

130 ical RNA-binding domain to recognize several low-affinity binding sites, including the well-character

131 nd binding to GPCRs have revealed transient, low-affinity binding sites, termed metastable binding si

132 inhibition by the NTD and to compensate for low-affinity binding sites, thereby allowing for full oc

133 how that there are two high-affinity and two low-affinity binding sites, to which the binding of cAMP

134 uffer from the inability to model and detect low-affinity binding sites, which have recently been sho

135 sotherms of [3H]8-OH-DPAT revealed high- and low-affinity binding sites.

136 UGT1A10-His or UGT1A7-His revealed high- and low-affinity binding sites.

137 es on HPASMCs, yet BMP2 competes only at the low-affinity binding sites.

138 rectly binds the flhDC promoter at high- and low-affinity binding sites.

139 rent K(d) values of 1.86 and 71.2 nm for the low-affinity binding sites.

140 nes and developed a species of specific, but low-affinity, binding sites for fMLP, in association wit

141 and catalytic domain (152-528), whereas the low-affinity binding state only requires residues in the

142 r (e.g., angiotensin IV) were shifted toward low-affinity binding states following peptide metabolism

143 finity binding to mispair-containing DNA and low affinity binding to fully base-paired DNA both invol

144 nduced cellular responses, initiated through low affinity binding to gp130, are mediated by two heter

145 (KD = 0.83 muM) compared to G7-B1 and shows low affinity binding to Grb2-, Grb10- and Grb14-SH2 doma

146 yrosine kinase receptors in conjunction with low affinity binding to heparin sulfate proteoglycans.

147 0 nM [3H]ryanodine reveal saturable high and low affinity binding to membrane preparations from wild-

148 nal residues, including F47, are involved in low affinity binding to MHC class II alpha-chain.

149 Low affinity binding to mono- and disulfated GSPs is lar

150 ow high affinity binding to site I, but only low affinity binding to site II, show that retardation o

151 Surprisingly, despite its low affinity binding to the isolated CNBD, cAMP also had

152 sion of DBP1 may have been selected to allow low-affinity binding to another receptor on Duffy-null e

153 tissue homogenates) had minimal influence on low-affinity binding to AT(1) and AT(2) receptors, yet c

154 altering glucose availability upon high- and low-affinity binding to brain SUR.

155 Domain 4 exhibited low-affinity binding to LRP6 in in vitro binding assays,

156 to the chaperone-adhesin (PapDG) complex and low-affinity binding to the major tip subunit PapE (PapD

157 toire was depleted of TCRs that demonstrated low-affinity binding to their ligands.

158 Yeast GAPDH and aldolase showed low-affinity binding to yeast actin, which suggests that

159 APD binding to its high affinity site, while low affinity binding was unaffected.

160 h avoided the inherent problem of relatively low affinity binding which hindered the use of eukaryoti

161 It may prevent nonspecific low-affinity binding while keeping the site available fo

162 proximately 3 pmol/mg total cell protein and low-affinity binding with a Kd of approximately 1.6 micr

163 t the RNA-binding surface allow for high- or low-affinity binding with functional implications.